##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve16.3420000000c86c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1319454 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.28502471476838 34.0 34.0 34.0 34.0 34.0 2 33.44768745253719 34.0 34.0 34.0 34.0 34.0 3 33.45839794339174 34.0 34.0 34.0 34.0 34.0 4 33.51963236308352 34.0 34.0 34.0 34.0 34.0 5 33.50202811162799 34.0 34.0 34.0 34.0 34.0 6 37.23395283200475 38.0 38.0 38.0 37.0 38.0 7 37.26210993335122 38.0 38.0 38.0 37.0 38.0 8 37.12388306072057 38.0 38.0 38.0 37.0 38.0 9 37.17897857750251 38.0 38.0 38.0 37.0 38.0 10-11 37.158013845120784 38.0 38.0 38.0 37.0 38.0 12-13 37.18151940120686 38.0 38.0 38.0 37.0 38.0 14-15 37.150212512145174 38.0 38.0 38.0 37.0 38.0 16-17 37.14426914466135 38.0 38.0 38.0 37.0 38.0 18-19 37.12751979227772 38.0 38.0 38.0 37.0 38.0 20-21 37.13511876882407 38.0 38.0 38.0 37.0 38.0 22-23 37.08769498595632 38.0 38.0 38.0 37.0 38.0 24-25 37.12115541731656 38.0 38.0 38.0 37.0 38.0 26-27 37.106362934971585 38.0 38.0 38.0 37.0 38.0 28-29 37.14584479640821 38.0 38.0 38.0 37.0 38.0 30-31 37.09844602388564 38.0 38.0 38.0 37.0 38.0 32-33 37.09398205621416 38.0 38.0 38.0 37.0 38.0 34-35 37.083894171376954 38.0 38.0 38.0 37.0 38.0 36-37 37.03395230148228 38.0 38.0 38.0 36.5 38.0 38-39 37.01775241880354 38.0 38.0 38.0 36.5 38.0 40-41 37.02466474769109 38.0 38.0 38.0 36.5 38.0 42-43 37.020375473491306 38.0 38.0 38.0 36.0 38.0 44-45 37.04192264376022 38.0 38.0 38.0 37.0 38.0 46-47 37.00017696713944 38.0 38.0 38.0 36.0 38.0 48-49 36.98662666527215 38.0 38.0 38.0 36.0 38.0 50-51 36.984349208081525 38.0 38.0 38.0 36.0 38.0 52-53 36.97017781597539 38.0 38.0 38.0 36.0 38.0 54-55 36.95498251549505 38.0 38.0 38.0 36.0 38.0 56-57 36.946909479223976 38.0 38.0 38.0 36.0 38.0 58-59 36.92464534572633 38.0 38.0 38.0 36.0 38.0 60-61 36.92900624046007 38.0 38.0 38.0 36.0 38.0 62-63 36.864327972024796 38.0 38.0 38.0 36.0 38.0 64-65 36.83802201516688 38.0 38.0 38.0 36.0 38.0 66-67 36.840080063420174 38.0 38.0 38.0 36.0 38.0 68-69 36.78991461619731 38.0 38.0 38.0 36.0 38.0 70-71 36.789964257943055 38.0 38.0 38.0 36.0 38.0 72-73 36.52267566735938 38.0 38.0 38.0 34.5 38.0 74-75 36.671477747613785 38.0 38.0 38.0 35.0 38.0 76 35.78465410692605 38.0 37.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17551334517695238 1101 2 0.17378171897038897 1101 3 0.17653133798602738 1101 4 0.09800117623718307 1101 5 0.17280902103667728 1101 6 0.1879978867805363 1101 7 0.08694788020994082 1101 8 0.26007480940753425 1101 9 0.24188170769462403 1101 10-11 0.24936467599260226 1101 12-13 0.19676537493610624 1101 14-15 0.1823332893138243 1101 16-17 0.19752304606210203 1101 18-19 0.14561095045388583 1101 20-21 0.15529958531244858 1101 22-23 0.1928801686233541 1101 24-25 0.15107028821157087 1101 26-27 0.16282516865921082 1101 28-29 0.17800880942175468 1101 30-31 0.19969900542258046 1101 32-33 0.20113527346761373 1101 34-35 0.23759288565254622 1101 36-37 0.19588949592062477 1101 38-39 0.18442350795933748 1101 40-41 0.13601121769256963 1101 42-43 0.1723953435305745 1101 44-45 0.17979999475233654 1101 46-47 0.16806114110625714 1101 48-49 0.07600889515695997 1101 50-51 0.15673013333540808 1101 52-53 0.15739687749759668 1101 54-55 0.18444689467115438 1101 56-57 0.15183099557369673 1101 58-59 0.17192723569537804 1101 60-61 0.08622960643254629 1101 62-63 0.23652168349460823 1101 64-65 0.19552418580762065 1101 66-67 0.19565585845167988 1101 68-69 0.24379981268697293 1101 70-71 0.23357188217548952 1101 72-73 0.4083118047877008 1101 74-75 0.26801086635062177 1101 76 0.28243820448743406 1102 1 0.1271760560251849 1102 2 0.1222222273069562 1102 3 0.10966803079733012 1102 4 0.17490588780700023 1102 5 0.10910556136835226 1102 6 0.1442880382264704 1102 7 0.16046183072208464 1102 8 0.24870611067951387 1102 9 0.23598907765445887 1102 10-11 0.23900324378848126 1102 12-13 0.17702993682726742 1102 14-15 0.18931429738395167 1102 16-17 0.11832995632892818 1102 18-19 0.18508574469908723 1102 20-21 0.13818868379266291 1102 22-23 0.18558450959778838 1102 24-25 0.11462217023192522 1102 26-27 0.17040121673991138 1102 28-29 0.08308850710341176 1102 30-31 0.1682964732764134 1102 32-33 0.025823340122762772 1102 34-35 0.1306944229198237 1102 36-37 0.11023178580931159 1102 38-39 0.2738636151565714 1102 40-41 0.31813089059229327 1102 42-43 0.2720858026833852 1102 44-45 0.27789569165773287 1102 46-47 0.2964817458629838 1102 48-49 0.2727328489435763 1102 50-51 0.2902091617179323 1102 52-53 0.2927581012959948 1102 54-55 0.27852423753490996 1102 56-57 0.18154144000139638 1102 58-59 0.2901191210102141 1102 60-61 0.2425529027241211 1102 62-63 0.27316099288140094 1102 64-65 0.24632778992612003 1102 66-67 0.25872726525363277 1102 68-69 0.2964804020783518 1102 70-71 0.2093223344992623 1102 72-73 0.5958358548077385 1102 74-75 0.34323599454515374 1102 76 0.5788999255252065 1103 1 0.021872907889068927 1103 2 0.08775829446334171 1103 3 0.07979596381945697 1103 4 0.08358900492706312 1103 5 0.06838960634490832 1103 6 0.09620724685111526 1103 7 0.07715253384672849 1103 8 0.16136902536173636 1103 9 0.12534975381232982 1103 10-11 0.12874743680993816 1103 12-13 0.14579656956397002 1103 14-15 0.17045601437131808 1103 16-17 0.1532797045449925 1103 18-19 0.165923109266366 1103 20-21 0.08830864458616361 1103 22-23 0.1753350931997062 1103 24-25 0.09271515375193928 1103 26-27 0.16911448689920405 1103 28-29 0.1015609762530687 1103 30-31 0.1306200637227306 1103 32-33 0.027588789248490286 1103 34-35 0.13739641087435928 1103 36-37 0.12492466550495607 1103 38-39 0.23584429640720828 1103 40-41 0.12856666747358503 1103 42-43 0.19920808246185118 1103 44-45 0.20906844274765746 1103 46-47 0.19038071857922034 1103 48-49 0.20937048974143835 1103 50-51 0.16619186728132718 1103 52-53 0.17328537914826114 1103 54-55 0.14309971595066173 1103 56-57 0.1843262646451862 1103 58-59 0.09486974234854273 1103 60-61 0.06952551658473993 1103 62-63 0.05747644112114614 1103 64-65 0.17452002022722013 1103 66-67 0.14389222590603623 1103 68-69 0.19114037889834634 1103 70-71 0.15896993377393187 1103 72-73 0.4266878545425925 1103 74-75 0.24811068666519986 1103 76 0.4142456764372824 2116 1 0.10039796074980956 2116 2 0.13469694710445168 2116 3 0.1241821289954288 2116 4 0.09563956821305197 2116 5 0.18735806329290483 2116 6 0.07567006612276828 2116 7 0.18814251077584743 2116 8 0.17325269801526844 2116 9 0.06399203951560395 2116 10-11 0.10637482035976831 2116 12-13 0.08617386762148271 2116 14-15 0.056070667994994494 2116 16-17 0.06672800698067505 2116 18-19 0.10847335707367023 2116 20-21 0.16534011057467524 2116 22-23 0.13018058089122064 2116 24-25 -0.018769715312366486 2116 26-27 0.06684894054309609 2116 28-29 0.009533556098077156 2116 30-31 0.07316380929761124 2116 32-33 0.2088750030707942 2116 34-35 0.1565722167899466 2116 36-37 0.04537828050536774 2116 38-39 0.1432316753332401 2116 40-41 0.0943208541888012 2116 42-43 0.10282946469196474 2116 44-45 0.06337882079226631 2116 46-47 0.05248799578143348 2116 48-49 0.07693735436495786 2116 50-51 0.04898840520380077 2116 52-53 0.00305464622625351 2116 54-55 -0.027400137147054693 2116 56-57 -0.04220645603336237 2116 58-59 -0.22663627101233885 2116 60-61 0.008772028257446607 2116 62-63 -0.02160551590632309 2116 64-65 -0.1823730686221765 2116 66-67 -0.014539002148929114 2116 68-69 0.026960334490510718 2116 70-71 0.011811048112079447 2116 72-73 0.28766627194738703 2116 74-75 -0.07940495481615528 2116 76 0.21595287368611338 1117 1 -0.004867692063442064 1117 2 0.06542405800917805 1117 3 0.104843455518143 1117 4 0.09894716824400973 1117 5 -0.019662484652300805 1117 6 0.012527700980747625 1117 7 0.10228615290880327 1117 8 0.24391383105451325 1117 9 0.1054961800620049 1117 10-11 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0.37011520616209737 1117 76 0.6094015231714494 2117 1 0.1735115330394592 2117 2 0.1452361757395053 2117 3 0.18855833070107053 2117 4 0.11139385913268285 2117 5 0.04213750978388475 2117 6 0.20808277886769133 2117 7 0.21674482605537548 2117 8 0.3407896357050859 2117 9 0.26081153192154716 2117 10-11 0.25676845149997973 2117 12-13 0.2449391345862466 2117 14-15 0.18319075290884967 2117 16-17 0.24245335290380154 2117 18-19 0.2664342316992432 2117 20-21 0.25944729031061087 2117 22-23 0.17659715670453124 2117 24-25 0.3012786971154071 2117 26-27 0.22759320957787565 2117 28-29 0.2601682888131265 2117 30-31 0.2201375122559881 2117 32-33 0.2517117968360978 2117 34-35 0.24692863407818066 2117 36-37 0.12337367940359911 2117 38-39 0.23108122058620495 2117 40-41 0.1977647164247074 2117 42-43 0.20728668196996125 2117 44-45 0.10074944482269643 2117 46-47 0.1803967437982621 2117 48-49 0.36201547223576824 2117 50-51 0.18152735207340243 2117 52-53 0.25652536423186945 2117 54-55 0.1963025010420978 2117 56-57 0.2520068660293546 2117 58-59 0.2508452261227845 2117 60-61 0.3105870140170879 2117 62-63 0.3761151856896561 2117 64-65 0.3469617567873087 2117 66-67 0.2502427977239563 2117 68-69 0.3590015891152305 2117 70-71 0.3326003355816809 2117 72-73 0.619419317335641 2117 74-75 0.4636101036261593 2117 76 0.8030572213590688 1116 1 0.11949937906099706 1116 2 0.10209089170097485 1116 3 0.05218717781445292 1116 4 0.09468401244554059 1116 5 -0.008637059573345596 1116 6 0.0407290908867779 1116 7 0.21929496412672478 1116 8 0.27366887585579036 1116 9 0.194752160203997 1116 10-11 0.11594540049705415 1116 12-13 0.21390557121638665 1116 14-15 0.15238992631456938 1116 16-17 0.21523196051118987 1116 18-19 0.30455933006820857 1116 20-21 0.23246142530240377 1116 22-23 0.21053159780857555 1116 24-25 0.2528368774024585 1116 26-27 0.14274981136900067 1116 28-29 0.19991162934410056 1116 30-31 0.18328600788660765 1116 32-33 0.32268032785801637 1116 34-35 0.23704110837069692 1116 36-37 0.08323726966057876 1116 38-39 0.15422326911313888 1116 40-41 0.17540113613418384 1116 42-43 0.17459717132634012 1116 44-45 0.10583206039814996 1116 46-47 0.23481879834653796 1116 48-49 0.33391874558802215 1116 50-51 0.17566464173524565 1116 52-53 0.29333002623891247 1116 54-55 0.1705010102829192 1116 56-57 0.3099472404461352 1116 58-59 0.318359103023127 1116 60-61 0.37839470216033533 1116 62-63 0.31856717087295294 1116 64-65 0.3545042940375467 1116 66-67 0.28291560458050213 1116 68-69 0.3358611830609135 1116 70-71 0.21794458428839647 1116 72-73 0.6286497742635504 1116 74-75 0.3223906665732841 1116 76 0.6439603070229438 2118 1 0.1776514546958623 2118 2 0.17923703595606355 2118 3 0.13330315874657828 2118 4 0.16811772459761443 2118 5 0.058424505050943765 2118 6 0.175733043716086 2118 7 0.19696151809649365 2118 8 0.22947874389599576 2118 9 0.19191414425377218 2118 10-11 0.09492326227981351 2118 12-13 0.20276076002271992 2118 14-15 0.03730318635220442 2118 16-17 0.22084688453412582 2118 18-19 0.13327600610342927 2118 20-21 0.07501189645402917 2118 22-23 0.06269087729093314 2118 24-25 0.21697122044095352 2118 26-27 0.1363653631616586 2118 28-29 0.20104250082233222 2118 30-31 0.16465067612659823 2118 32-33 0.19020738816647054 2118 34-35 0.2036176702524486 2118 36-37 0.2291982011202478 2118 38-39 0.17602596652955071 2118 40-41 0.1477006781692083 2118 42-43 0.19778430824864301 2118 44-45 0.192599669631754 2118 46-47 0.22057832931648846 2118 48-49 0.25381798601513594 2118 50-51 0.17357222099472835 2118 52-53 0.18924400944575126 2118 54-55 0.11531225141119705 2118 56-57 0.2890803834762252 2118 58-59 0.3138077329330926 2118 60-61 0.2939514071856095 2118 62-63 0.2523623821986263 2118 64-65 0.31837106763179435 2118 66-67 0.21225951243757635 2118 68-69 0.2743431906077589 2118 70-71 0.18871927713132663 2118 72-73 0.5545954908505095 2118 74-75 0.40661231200394354 2118 76 0.6885893873482019 1119 1 0.2702115967648453 1119 2 0.21621617148259986 1119 3 0.11011215941697827 1119 4 0.09744920341002228 1119 5 0.0710496168374064 1119 6 0.1345485588497155 1119 7 0.1598041284868117 1119 8 0.10316712141175088 1119 9 -0.039815448277423116 1119 10-11 0.06395288744058547 1119 12-13 0.1930776754288388 1119 14-15 0.024330312139476007 1119 16-17 0.14090116059674784 1119 18-19 0.1833853199031239 1119 20-21 0.17315368906994877 1119 22-23 0.15728404613651037 1119 24-25 0.0975610999341967 1119 26-27 0.04582019984212593 1119 28-29 0.15151370539378206 1119 30-31 0.17967041128650862 1119 32-33 0.18544545198047757 1119 34-35 0.13871616954705956 1119 36-37 0.21117895540374576 1119 38-39 0.1531448844239094 1119 40-41 0.22706492965470915 1119 42-43 0.25792299819403297 1119 44-45 0.233152672314624 1119 46-47 0.3059217649866355 1119 48-49 0.24328254954863837 1119 50-51 0.24160254994905017 1119 52-53 0.19460371696276724 1119 54-55 0.21485578738703737 1119 56-57 0.29349940714633505 1119 58-59 0.32065982147941696 1119 60-61 0.22516755322102 1119 62-63 0.2996029740085504 1119 64-65 0.2947348703813262 1119 66-67 0.2570225951105769 1119 68-69 0.29723476760143086 1119 70-71 0.28460931996319516 1119 72-73 0.5661403049764999 1119 74-75 0.49081307698587295 1119 76 0.06492358974306711 2119 1 0.1269941281504856 2119 2 0.07295852760622523 2119 3 0.09343350594653543 2119 4 0.055788088138434944 2119 5 0.04798230740863829 2119 6 0.11534652006172053 2119 7 0.03316025779527365 2119 8 0.1219947962159722 2119 9 0.09439400043904556 2119 10-11 0.03068196915248933 2119 12-13 0.1141176180058352 2119 14-15 0.061168185935166264 2119 16-17 0.17603726870151348 2119 18-19 0.16260298836770204 2119 20-21 0.2015849484862713 2119 22-23 0.1352023724335112 2119 24-25 0.1235085573215855 2119 26-27 0.14934810089069117 2119 28-29 0.11703441151860261 2119 30-31 0.16232681381832492 2119 32-33 0.24420966565667612 2119 34-35 0.13263810230393602 2119 36-37 0.25290960781843097 2119 38-39 0.1715749898867358 2119 40-41 0.1862500206386528 2119 42-43 0.06377967527521378 2119 44-45 0.10442162177809422 2119 46-47 0.10034609373614956 2119 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-0.06619133403010125 2104 56-57 -0.3492365862182183 2104 58-59 -0.09199813369513521 2104 60-61 -0.2373931089957111 2104 62-63 -0.2959422774902123 2104 64-65 -0.2859175644575416 2104 66-67 -0.16588025592320577 2104 68-69 -0.5461398433253564 2104 70-71 -0.29563197446216094 2104 72-73 -0.19088401152362167 2104 74-75 -0.08457750024294342 2104 76 -0.25396250369235673 2111 1 -0.20691884896010748 2111 2 -0.1084828029462841 2111 3 -0.09989987374105169 2111 4 -0.06838849052306273 2111 5 -0.04348396065456228 2111 6 -0.11399514065166017 2111 7 -0.18801607411067067 2111 8 0.021138800233913457 2111 9 -0.10819570393615408 2111 10-11 -0.12540009437073962 2111 12-13 -0.11870416918350202 2111 14-15 -0.06433656338156624 2111 16-17 -0.15558428557387316 2111 18-19 -0.04127999969650631 2111 20-21 -0.08412863267415105 2111 22-23 -0.23701734252662732 2111 24-25 -0.07870402016143885 2111 26-27 -0.19238275024779483 2111 28-29 -0.15648041435056115 2111 30-31 -0.1493560105399041 2111 32-33 -0.09432072068878483 2111 34-35 -0.13139349210884887 2111 36-37 -0.19941717091557365 2111 38-39 -0.06877053741823858 2111 40-41 -0.1523688565168584 2111 42-43 -0.17308705958610204 2111 44-45 -0.26424358429837724 2111 46-47 -0.08594775913997665 2111 48-49 -0.30173430735902684 2111 50-51 -0.10169538737366679 2111 52-53 -0.11818367627245863 2111 54-55 -0.15107543223513176 2111 56-57 -0.2870701557800075 2111 58-59 -0.12343301866047085 2111 60-61 -0.3177515296897937 2111 62-63 -0.23874026461500364 2111 64-65 -0.2963125845664223 2111 66-67 -0.23373953111437373 2111 68-69 -0.4751576549170906 2111 70-71 -0.2736689160125465 2111 72-73 -0.11918425603606408 2111 74-75 -0.1057951257479317 2111 76 -0.3882968529932569 2110 1 -0.053596680291526866 2110 2 -0.10700084361022277 2110 3 -0.08738188052090123 2110 4 -0.08411775256463727 2110 5 0.017246225552412398 2110 6 0.028433078384438204 2110 7 -0.11600192729653713 2110 8 -0.26440153670262134 2110 9 -0.2896087116381949 2110 10-11 -0.24371694538177024 2110 12-13 -0.23247321217976946 2110 14-15 -0.20298389989077492 2110 16-17 -0.19757009955272764 2110 18-19 -0.219189415673398 2110 20-21 -0.2665647293553519 2110 22-23 -0.2795954625073449 2110 24-25 -0.20202586100664632 2110 26-27 -0.17222025363722793 2110 28-29 -0.2154833914480605 2110 30-31 -0.24913012540856272 2110 32-33 -0.23706461386647248 2110 34-35 -0.23923362698098316 2110 36-37 -0.33077772687766327 2110 38-39 -0.37633603372800195 2110 40-41 -0.25293460717802674 2110 42-43 -0.2817705571550988 2110 44-45 -0.3051619954850864 2110 46-47 -0.08447094778404107 2110 48-49 -0.15120296167866343 2110 50-51 -0.17620898150065045 2110 52-53 -0.09889283565754425 2110 54-55 -0.21895616284306385 2110 56-57 -0.29840588447536476 2110 58-59 -0.18164077697128533 2110 60-61 -0.318601069962817 2110 62-63 -0.36168626172685947 2110 64-65 -0.48556225946118303 2110 66-67 -0.3072201521403386 2110 68-69 -0.3721455017303299 2110 70-71 -0.47158120003212645 2110 72-73 0.04692388910633838 2110 74-75 -0.1676412293117835 2110 76 -0.3502192619777915 2109 1 0.0015217898397423824 2109 2 -0.13969711457774991 2109 3 -0.09972522542039997 2109 4 -0.31591055774385524 2109 5 -0.1248352989004573 2109 6 -0.3701036516848504 2109 7 -0.16808685308063787 2109 8 -0.8812707446437642 2109 9 -0.4042435854996782 2109 10-11 -0.3841949206336963 2109 12-13 -0.6308614981426928 2109 14-15 -0.343391263670334 2109 16-17 -0.4169501792398833 2109 18-19 -0.5050437755112611 2109 20-21 -0.5344678968604626 2109 22-23 -0.25677187343411845 2109 24-25 -0.3295971176651946 2109 26-27 -0.09764038369198857 2109 28-29 -0.32476402014865613 2109 30-31 -0.35154392176605853 2109 32-33 -0.4157654694510384 2109 34-35 -0.21048577230194354 2109 36-37 -0.21707922432502613 2109 38-39 -0.1598359256157238 2109 40-41 -0.4976900539238045 2109 42-43 -0.3836925349656184 2109 44-45 -0.4175717258362468 2109 46-47 -0.28123237988809535 2109 48-49 -0.7231531389458752 2109 50-51 -0.40770198538490376 2109 52-53 -0.6547870883103926 2109 54-55 -0.5173517109969339 2109 56-57 -0.670294845986156 2109 58-59 -0.4679476902898614 2109 60-61 -0.6468128764799914 2109 62-63 -0.6278831836246681 2109 64-65 -0.793085520012589 2109 66-67 -0.4821338366929169 2109 68-69 -0.9775649424923927 2109 70-71 -0.37003587144671 2109 72-73 -0.005060022473820425 2109 74-75 -0.316070031735471 2109 76 -1.3493404958445439 2108 1 -0.01377651840414984 2108 2 -0.04102415238621404 2108 3 -0.12728235651913877 2108 4 -0.12227546966592229 2108 5 -0.07328595244756286 2108 6 -0.12761085318859955 2108 7 -0.03469817630008265 2108 8 -0.5095225772722145 2108 9 -0.13909171718051994 2108 10-11 -0.19028980473031254 2108 12-13 -0.3385457612833278 2108 14-15 -0.03482129762844011 2108 16-17 -0.14072879049330567 2108 18-19 -0.19043659557151926 2108 20-21 -0.2775312896215141 2108 22-23 0.05273573192721415 2108 24-25 -0.09239726341955645 2108 26-27 0.007752984816839614 2108 28-29 -0.05407851344241976 2108 30-31 -0.0703222193235078 2108 32-33 -0.14159590161604285 2108 34-35 -0.044717148629487724 2108 36-37 -0.29624499249165837 2108 38-39 -0.20725362612873255 2108 40-41 -0.16038225502659742 2108 42-43 0.005040752414373628 2108 44-45 -0.05888847607899805 2108 46-47 -0.049477082685065454 2108 48-49 -0.3101075001300089 2108 50-51 -0.1514433353744522 2108 52-53 -0.22745403169430034 2108 54-55 -0.061640304637663235 2108 56-57 -0.23812875248980703 2108 58-59 -0.013311201590603616 2108 60-61 -0.2263131567289136 2108 62-63 -0.08922387447874769 2108 64-65 -0.18589336482158814 2108 66-67 -0.06988012438161917 2108 68-69 -0.2565296959460639 2108 70-71 -0.1647881326544791 2108 72-73 0.23550244478026627 2108 74-75 -0.050359867695334515 2108 76 -0.43007719523946264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 15.0 13 63.0 14 257.0 15 506.0 16 882.0 17 1260.0 18 1558.0 19 1703.0 20 1831.0 21 2015.0 22 2214.0 23 2471.0 24 2974.0 25 3553.0 26 4197.0 27 5025.0 28 6074.0 29 7502.0 30 9538.0 31 11999.0 32 15440.0 33 21026.0 34 30091.0 35 48405.0 36 105950.0 37 1032903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58981972846344 26.43866326525972 8.520569872083453 24.450947134193385 2 15.145204001048917 23.252421077203145 41.3722645882312 20.230110333516745 3 18.372599575278866 25.94755103247252 32.66927077412324 23.010578618125376 4 13.434799545872764 16.243764466211026 41.757044959505976 28.564391028410235 5 12.496229500990562 35.58578017877092 37.843608037870204 14.074382282368314 6 26.932807055039433 38.77861600328621 19.095398551218913 15.193178390455445 7 23.274096709699617 34.82986144268766 23.943540282571426 17.9525015650413 8 20.23064085599043 41.27275372995193 22.893560518214354 15.60304489584328 9 20.954273510103423 17.99774755315456 25.70934644178577 35.338632494956244 10-11 23.21047190731924 26.73859793520653 31.015253279007833 19.035676878466397 12-13 20.476462233620875 25.152979944734717 31.789929773982266 22.580628047662138 14-15 19.837940542072705 28.97452279503492 26.02504520809365 25.16249145479873 16-17 18.97925202394324 33.90777548895225 28.228153463478073 18.884819023626438 18-19 18.631873487063586 32.0604583411017 31.008129120075424 18.29953905175929 20-21 18.852760308430607 29.99107964354953 31.5805628691868 19.575597178833064 22-23 19.13757508787726 30.345506550436774 31.230380142089075 19.286538219596892 24-25 19.082666011850353 30.818239968956856 31.1520143938326 18.947079625360185 26-27 18.820436331997932 30.965005221856917 31.11415782588859 19.10040062025656 28-29 18.682197333139314 31.01972482557179 31.28555447935282 19.012523361936072 30-31 18.83419202185146 30.6880345961284 31.43978494134695 19.037988440673185 32-33 18.84120249739665 30.742451044144016 31.27437561294293 19.141970845516404 34-35 18.80330803499023 30.678219930365135 31.54850415399097 18.969967880653666 36-37 18.875648563724084 30.916727676751144 31.56263878846856 18.64498497105621 38-39 18.754083128324293 30.71649333739562 31.397418932376574 19.132004601903514 40-41 18.800503844772155 30.701297657970645 31.28354607284528 19.214652424411916 42-43 19.03613161201527 30.70285133092931 31.219693903690466 19.041323153364953 44-45 18.654989109131503 30.511484295776892 31.592916463931292 19.240610131160313 46-47 18.788301827877287 30.414815522178113 31.7564689636774 19.0404136862672 48-49 18.645856543691558 30.630586591120267 31.657602311259055 19.065954553929128 50-51 18.552105643698074 30.622590859552513 31.68291581214654 19.142387684602873 52-53 18.401588839019777 30.418150234869877 31.96394114535255 19.2163197807578 54-55 18.34785449132747 30.300298456785914 32.07220562444768 19.279641427438925 56-57 18.244023664333884 30.364605359489605 32.07644980423721 19.314921171939304 58-59 18.069254403715476 30.50083595184069 32.1661459967532 19.263763647690634 60-61 18.372296419579616 30.22022745772115 32.0404121704887 19.367063952210536 62-63 18.136024446475588 29.804449416197915 32.283959880374766 19.77556625695174 64-65 18.21177547682602 29.621116007075653 32.28297462435219 19.88413389174613 66-67 18.280553926093674 29.690955501290684 31.973301077566934 20.055189495048708 68-69 18.15281169332163 29.72434052267074 31.90656892926923 20.2162788547384 70-71 18.340313493308596 29.641541122312713 31.892964817265323 20.125180567113365 72-73 18.22871430152169 29.159182510341395 32.10521170120368 20.50689148693323 74-75 18.483706139054487 29.260739669590603 31.73953013898173 20.516024052373176 76 18.434215971151705 29.08960827736321 31.6702969561652 20.80587879531988 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 4.0 2 3.5 3 5.0 4 4.0 5 6.0 6 12.0 7 21.0 8 26.0 9 47.0 10 119.5 11 273.0 12 375.0 13 528.0 14 880.0 15 1477.0 16 2512.5 17 3150.0 18 4222.5 19 6620.5 20 9691.0 21 11436.0 22 13645.0 23 18621.0 24 24283.0 25 27178.0 26 30116.0 27 36396.0 28 42569.5 29 45401.0 30 48448.5 31 54069.0 32 58264.0 33 59886.0 34 61389.0 35 64986.5 36 68175.0 37 69269.0 38 69779.0 39 70763.0 40 71052.5 41 69844.0 42 68820.0 43 67985.5 44 65276.5 45 60745.5 46 58089.0 47 54645.5 48 47489.5 49 40544.5 50 37312.0 51 34574.0 52 28355.0 53 22210.5 54 19547.0 55 17511.5 56 13621.0 57 10168.0 58 8570.0 59 7778.0 60 5981.5 61 4160.5 62 3344.0 63 2952.0 64 2104.5 65 1381.0 66 960.5 67 808.0 68 687.5 69 472.0 70 304.0 71 231.0 72 188.5 73 128.5 74 89.5 75 68.0 76 53.0 77 34.0 78 25.0 79 20.0 80 16.0 81 10.5 82 6.0 83 3.0 84 6.0 85 7.5 86 4.5 87 3.0 88 3.0 89 5.5 90 9.0 91 9.0 92 8.0 93 5.5 94 4.5 95 7.5 96 9.0 97 7.5 98 7.5 99 47.5 100 86.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1319454.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.07524160247138 #Duplication Level Percentage of deduplicated Percentage of total 1 95.83068540590877 88.23633511679478 2 3.0963563664207405 5.7019552105108025 3 0.46768428012856667 1.2918642925954704 4 0.18787061915514547 0.6919293059486363 5 0.09867489794578789 0.45427575342288135 6 0.054985839098317224 0.30377006518153055 7 0.049243415237143505 0.3173869548703567 8 0.04321855127636791 0.3183486840384298 9 0.030826222658033454 0.2554498709037007 >10 0.1359455662389199 2.0731605796723134 >50 0.0036005426234228264 0.21191767221429061 >100 9.082933087445541E-4 0.14360649384676016 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.515778496256785E-4 0.0 0.0 2 1.515778496256785E-4 0.0 0.0 3 1.515778496256785E-4 7.578892481283925E-5 0.0 4 1.515778496256785E-4 7.578892481283925E-5 0.0 5 1.515778496256785E-4 7.578892481283925E-5 0.0 6 1.515778496256785E-4 7.578892481283925E-5 0.0 7 1.515778496256785E-4 7.578892481283925E-5 7.578892481283925E-5 8 1.515778496256785E-4 7.578892481283925E-5 7.578892481283925E-5 9 1.515778496256785E-4 7.578892481283925E-5 7.578892481283925E-5 10 2.2736677443851774E-4 1.515778496256785E-4 7.578892481283925E-5 11 2.2736677443851774E-4 1.515778496256785E-4 7.578892481283925E-5 12 2.2736677443851774E-4 1.515778496256785E-4 7.578892481283925E-5 13 3.7894462406419624E-4 1.515778496256785E-4 7.578892481283925E-5 14 3.7894462406419624E-4 1.515778496256785E-4 7.578892481283925E-5 15 4.547335488770355E-4 1.515778496256785E-4 7.578892481283925E-5 16 4.547335488770355E-4 1.515778496256785E-4 7.578892481283925E-5 17 4.547335488770355E-4 2.2736677443851774E-4 7.578892481283925E-5 18 4.547335488770355E-4 2.2736677443851774E-4 7.578892481283925E-5 19 4.547335488770355E-4 2.2736677443851774E-4 7.578892481283925E-5 20 4.547335488770355E-4 2.2736677443851774E-4 7.578892481283925E-5 21 4.547335488770355E-4 2.2736677443851774E-4 3.03155699251357E-4 22 5.305224736898747E-4 2.2736677443851774E-4 6.821003233155533E-4 23 5.305224736898747E-4 2.2736677443851774E-4 7.578892481283925E-4 24 5.305224736898747E-4 2.2736677443851774E-4 9.09467097754071E-4 25 5.305224736898747E-4 2.2736677443851774E-4 0.0010610449473797494 26 5.305224736898747E-4 3.03155699251357E-4 0.0015915674210696242 27 5.305224736898747E-4 3.03155699251357E-4 0.004774702263208873 28 5.305224736898747E-4 3.7894462406419624E-4 0.012808328293369833 29 5.305224736898747E-4 3.7894462406419624E-4 0.022054577120536222 30 5.305224736898747E-4 3.7894462406419624E-4 0.03137661487251545 31 6.06311398502714E-4 3.7894462406419624E-4 0.038121829180858144 32 8.336781729412318E-4 4.547335488770355E-4 0.046079666286206265 33 8.336781729412318E-4 4.547335488770355E-4 0.05593222651187537 34 9.09467097754071E-4 4.547335488770355E-4 0.07162053394813309 35 9.09467097754071E-4 4.547335488770355E-4 0.09534246741455178 36 9.09467097754071E-4 4.547335488770355E-4 0.14051266660300396 37 9.852560225669103E-4 4.547335488770355E-4 0.20857112108493361 38 9.852560225669103E-4 4.547335488770355E-4 0.2971683741911427 39 9.852560225669103E-4 4.547335488770355E-4 0.40403075817724604 40 9.852560225669103E-4 4.547335488770355E-4 0.5319624632613187 41 0.0010610449473797494 4.547335488770355E-4 0.6627741474882792 42 0.0010610449473797494 5.305224736898747E-4 0.8064699489334225 43 0.0011368338721925888 5.305224736898747E-4 0.9506962728522556 44 0.001212622797005428 6.06311398502714E-4 1.1218276650796466 45 0.001212622797005428 6.06311398502714E-4 1.3125125999087501 46 0.001212622797005428 6.06311398502714E-4 1.5467003775804233 47 0.0012884117218182672 6.06311398502714E-4 1.7940754281695308 48 0.0014399895714439458 6.06311398502714E-4 2.060776654585912 49 0.0014399895714439458 6.821003233155533E-4 2.342256721340797 50 0.0014399895714439458 7.578892481283925E-4 2.6474587215621006 51 0.0014399895714439458 8.336781729412318E-4 2.955161756302228 52 0.0014399895714439458 8.336781729412318E-4 3.2721868287943345 53 0.0014399895714439458 8.336781729412318E-4 3.5852708771961734 54 0.0014399895714439458 8.336781729412318E-4 3.912224298838762 55 0.0014399895714439458 8.336781729412318E-4 4.255244972541672 56 0.0016673563458824636 8.336781729412318E-4 4.619789700891429 57 0.0016673563458824636 8.336781729412318E-4 5.004797438940653 58 0.0016673563458824636 8.336781729412318E-4 5.416331300674369 59 0.0017431452706953027 8.336781729412318E-4 5.841734535648836 60 0.0017431452706953027 8.336781729412318E-4 6.303289087759028 61 0.0018947231203209813 8.336781729412318E-4 6.758401581260127 62 0.0018947231203209813 8.336781729412318E-4 7.221168756167324 63 0.0018947231203209813 8.336781729412318E-4 7.677493872465429 64 0.0018947231203209813 8.336781729412318E-4 8.133212677365032 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 95 2.781709E-4 22.105263 6 CCCGGAC 105 5.4671505E-4 20.0 3 CCTACGA 125 7.8274825E-5 19.6 2 GTCTTAC 235 3.783498E-10 19.361702 1 GTATAGG 240 5.020411E-10 18.958332 1 GCCGTAG 155 1.984964E-5 18.064518 1 TACACTG 525 0.0 18.0 5 AGGGCTG 235 7.936251E-9 17.872341 5 TACGCCT 240 1.0299118E-8 17.5 4 CGCACCG 120 0.0013412109 17.5 5 GTGTTAC 345 0.0 17.246376 1 GTACAGG 205 6.672235E-7 17.073172 1 TAGGGCT 205 6.672235E-7 17.073172 4 GTATAGA 415 0.0 16.86747 1 GTATTAG 270 2.413799E-9 16.851852 1 CTTACAC 355 1.8189894E-12 16.760561 3 ACAGTCC 210 8.584757E-7 16.666666 8 GCCCTGA 210 8.584757E-7 16.666666 1 TACACAG 340 1.0913936E-11 16.470589 5 ACAGGGC 150 3.1595823E-4 16.333332 8 >>END_MODULE