FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve16.3420000000c86c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve16.3420000000c86c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319454
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC952.781709E-422.1052636
CCCGGAC1055.4671505E-420.03
CCTACGA1257.8274825E-519.62
GTCTTAC2353.783498E-1019.3617021
GTATAGG2405.020411E-1018.9583321
GCCGTAG1551.984964E-518.0645181
TACACTG5250.018.05
AGGGCTG2357.936251E-917.8723415
TACGCCT2401.0299118E-817.54
CGCACCG1200.001341210917.55
GTGTTAC3450.017.2463761
GTACAGG2056.672235E-717.0731721
TAGGGCT2056.672235E-717.0731724
GTATAGA4150.016.867471
GTATTAG2702.413799E-916.8518521
CTTACAC3551.8189894E-1216.7605613
ACAGTCC2108.584757E-716.6666668
GCCCTGA2108.584757E-716.6666661
TACACAG3401.0913936E-1116.4705895
ACAGGGC1503.1595823E-416.3333328