##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve15.3420000000c843.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1210567 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.19966594166205 34.0 34.0 34.0 34.0 34.0 2 33.37684489995184 34.0 34.0 34.0 34.0 34.0 3 33.4335084303471 34.0 34.0 34.0 34.0 34.0 4 33.469191709339505 34.0 34.0 34.0 34.0 34.0 5 33.45953507736457 34.0 34.0 34.0 34.0 34.0 6 37.126154107951066 38.0 38.0 38.0 37.0 38.0 7 37.151548819685324 38.0 38.0 38.0 37.0 38.0 8 37.10700770795834 38.0 38.0 38.0 37.0 38.0 9 37.159678068211015 38.0 38.0 38.0 37.0 38.0 10-11 37.12175079941879 38.0 38.0 38.0 37.0 38.0 12-13 37.15388202387807 38.0 38.0 38.0 37.0 38.0 14-15 37.12776120611251 38.0 38.0 38.0 37.0 38.0 16-17 37.13048265812631 38.0 38.0 38.0 37.0 38.0 18-19 37.11042759302046 38.0 38.0 38.0 37.0 38.0 20-21 37.11110661367772 38.0 38.0 38.0 37.0 38.0 22-23 37.069939953757206 38.0 38.0 38.0 37.0 38.0 24-25 37.10980391832918 38.0 38.0 38.0 37.0 38.0 26-27 37.08912889579842 38.0 38.0 38.0 37.0 38.0 28-29 37.133370561067665 38.0 38.0 38.0 37.0 38.0 30-31 37.08959933650925 38.0 38.0 38.0 37.0 38.0 32-33 37.074783964869354 38.0 38.0 38.0 37.0 38.0 34-35 37.0741437689942 38.0 38.0 38.0 37.0 38.0 36-37 37.029405642149506 38.0 38.0 38.0 36.5 38.0 38-39 37.01513051322232 38.0 38.0 38.0 36.0 38.0 40-41 37.01031706630034 38.0 38.0 38.0 36.0 38.0 42-43 37.01516272953087 38.0 38.0 38.0 36.0 38.0 44-45 37.02724095403229 38.0 38.0 38.0 36.0 38.0 46-47 36.97826968684922 38.0 38.0 38.0 36.0 38.0 48-49 36.965504594128205 38.0 38.0 38.0 36.0 38.0 50-51 36.95937110461462 38.0 38.0 38.0 36.0 38.0 52-53 36.94702399784563 38.0 38.0 38.0 36.0 38.0 54-55 36.92683304600241 38.0 38.0 38.0 36.0 38.0 56-57 36.916872424244175 38.0 38.0 38.0 36.0 38.0 58-59 36.89601732080917 38.0 38.0 38.0 36.0 38.0 60-61 36.90276374624453 38.0 38.0 38.0 36.0 38.0 62-63 36.83243678375506 38.0 38.0 38.0 36.0 38.0 64-65 36.802456204406695 38.0 38.0 38.0 36.0 38.0 66-67 36.80064713477238 38.0 38.0 38.0 36.0 38.0 68-69 36.75840122851523 38.0 38.0 38.0 36.0 38.0 70-71 36.75621588891816 38.0 38.0 38.0 36.0 38.0 72-73 36.48678718319597 38.0 38.0 38.0 34.0 38.0 74-75 36.62830681820998 38.0 38.0 38.0 35.0 38.0 76 35.706246742229055 38.0 37.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.00918938063297503 1101 2 0.0834802815213962 1101 3 0.025555083483538965 1101 4 0.07148524835476167 1101 5 0.07371616394705427 1101 6 0.07398248603536928 1101 7 0.015403045291343176 1101 8 0.2549854221830756 1101 9 0.0905054931343372 1101 10-11 0.1566921768088747 1101 12-13 0.1095089594863623 1101 14-15 0.05906720985301206 1101 16-17 0.0977735697603066 1101 18-19 0.09703236320481778 1101 20-21 0.07085641332587045 1101 22-23 0.032139712484848815 1101 24-25 0.02637369339064577 1101 26-27 0.056734675426568515 1101 28-29 0.0674372454713108 1101 30-31 0.11465890057116468 1101 32-33 0.1521205257090159 1101 34-35 0.09396058636001925 1101 36-37 0.06872738093761654 1101 38-39 0.1596369915891529 1101 40-41 0.09256210821527588 1101 42-43 0.055396237653788205 1101 44-45 0.03629849780939054 1101 46-47 0.12520518661588653 1101 48-49 0.12273184448065422 1101 50-51 0.12765609155523805 1101 52-53 0.06943677899692347 1101 54-55 0.15105154340957228 1101 56-57 0.08822910266042783 1101 58-59 0.14443212147472195 1101 60-61 0.02591719311562457 1101 62-63 0.17460418908063247 1101 64-65 0.006204087743263642 1101 66-67 0.12413132970340257 1101 68-69 0.20612683089859019 1101 70-71 0.09372081300075763 1101 72-73 0.43960134417108776 1101 74-75 0.2538132053062796 1101 76 0.14990118120713447 1102 1 0.10091003789184327 1102 2 0.07949594463779164 1102 3 0.08563108134499942 1102 4 0.12489049594671542 1102 5 0.08512665270367137 1102 6 0.1464090128337716 1102 7 0.12223233543586787 1102 8 0.23416966246252713 1102 9 0.1756010893679445 1102 10-11 0.22203261392317586 1102 12-13 0.12147083200978415 1102 14-15 0.13658911864195034 1102 16-17 0.10571633959216342 1102 18-19 0.09116127521032524 1102 20-21 0.08733014761186553 1102 22-23 0.17645345783358835 1102 24-25 0.06359492176305537 1102 26-27 0.13020670842779225 1102 28-29 0.0630753538607749 1102 30-31 0.11825312029301926 1102 32-33 0.09864170509766979 1102 34-35 0.12032826058589308 1102 36-37 0.07466834138932654 1102 38-39 0.2291883763297946 1102 40-41 0.19703375070980655 1102 42-43 0.25950050196741614 1102 44-45 0.2189121367182736 1102 46-47 0.25111214084869715 1102 48-49 0.33304263327496386 1102 50-51 0.24321164819283325 1102 52-53 0.2527981749268875 1102 54-55 0.3262606008276876 1102 56-57 0.23625339198206774 1102 58-59 0.2728148491980704 1102 60-61 0.21376274528333283 1102 62-63 0.22057296217871425 1102 64-65 0.2614716432590001 1102 66-67 0.28121849060288184 1102 68-69 0.3968639828875027 1102 70-71 0.23278156162449193 1102 72-73 0.5445696410652516 1102 74-75 0.3117392252250468 1102 76 0.5716224100664604 1103 1 0.061430585666563786 1103 2 0.03371360797432743 1103 3 0.016379195252042678 1103 4 0.04298392055349609 1103 5 0.1027604072217514 1103 6 0.1839037893100368 1103 7 0.160591883319249 1103 8 0.2295160951727695 1103 9 0.17354978227836426 1103 10-11 0.14483245136568001 1103 12-13 0.15890376387972083 1103 14-15 0.123494528465379 1103 16-17 0.07961384699603968 1103 18-19 0.12890070167340184 1103 20-21 0.0115849607928169 1103 22-23 0.11397116276684471 1103 24-25 -0.02115530883787642 1103 26-27 0.18381688732241486 1103 28-29 0.11426789689471661 1103 30-31 0.13015887715468466 1103 32-33 0.02010345885178566 1103 34-35 0.14250979556710774 1103 36-37 0.08972300350093576 1103 38-39 0.17388934186195115 1103 40-41 0.07653810352657331 1103 42-43 0.16751478749707616 1103 44-45 0.19783767763120608 1103 46-47 0.22453241084922837 1103 48-49 0.1477095699134381 1103 50-51 0.13513255028161808 1103 52-53 0.16506882453938232 1103 54-55 0.04887859023656205 1103 56-57 0.11738671832676317 1103 58-59 0.1395189002591195 1103 60-61 0.10001017061864559 1103 62-63 0.0662474537198463 1103 64-65 0.17322522983929645 1103 66-67 0.13952708848704276 1103 68-69 0.18423873200278962 1103 70-71 0.15729959732108512 1103 72-73 0.45962243635050726 1103 74-75 0.15158055455858488 1103 76 0.37502328900659876 2116 1 -0.009715226319322312 2116 2 0.06897287439737454 2116 3 0.023506939910099334 2116 4 0.05635596136559684 2116 5 0.11095322158473664 2116 6 0.06127493899813885 2116 7 0.15120025389787628 2116 8 0.1560821246972992 2116 9 0.14860656169025077 2116 10-11 0.09950587284430412 2116 12-13 0.05926112493997948 2116 14-15 0.057245293135729014 2116 16-17 0.04593150928845091 2116 18-19 0.09394101908547015 2116 20-21 0.015933939454200186 2116 22-23 0.057642349911319 2116 24-25 -0.05027504949853068 2116 26-27 0.06961135582950817 2116 28-29 -0.01797910563589511 2116 30-31 0.11217665127892928 2116 32-33 0.15888810503872008 2116 34-35 0.08648387660029755 2116 36-37 0.023496746659191103 2116 38-39 0.11238173430344034 2116 40-41 0.03663449612464831 2116 42-43 0.014341626217451164 2116 44-45 0.05829618049685337 2116 46-47 0.014864550260618614 2116 48-49 -0.021823603155731064 2116 50-51 -0.0759991215391338 2116 52-53 -0.06408215535161332 2116 54-55 -0.035555455842455785 2116 56-57 -0.038506539449073784 2116 58-59 -0.22480094801186112 2116 60-61 -0.08834435089535475 2116 62-63 -0.085102875443404 2116 64-65 -0.07501262235999917 2116 66-67 -0.06710199899738711 2116 68-69 0.07987370198012655 2116 70-71 -0.1274283335442732 2116 72-73 0.22113149265711485 2116 74-75 -0.18182017914118376 2116 76 0.21657486175217144 1117 1 -0.018196229012218623 1117 2 0.06420338370134004 1117 3 0.05670186578019809 1117 4 0.09812923631810833 1117 5 -0.028090225199107977 1117 6 -0.022058876725630228 1117 7 0.0342071632725407 1117 8 0.21414389151207303 1117 9 0.03834040566280095 1117 10-11 -0.10002369035694869 1117 12-13 0.1696248685521624 1117 14-15 0.18069575858460496 1117 16-17 0.1498484424275901 1117 18-19 -0.04014166919537132 1117 20-21 -0.0617999504419231 1117 22-23 -0.36571232244523344 1117 24-25 -0.1775856996759373 1117 26-27 -0.1077011370793528 1117 28-29 -0.09782736104195067 1117 30-31 -0.07857258596531835 1117 32-33 0.18130840642082546 1117 34-35 0.2008723000788919 1117 36-37 -0.005933170114943209 1117 38-39 0.06048568905598728 1117 40-41 0.09418956002787127 1117 42-43 0.08690974167624432 1117 44-45 0.13364865353894118 1117 46-47 0.14396337353669253 1117 48-49 0.08845113942143001 1117 50-51 0.12455976636953636 1117 52-53 0.1398890177678922 1117 54-55 0.16343151459897598 1117 56-57 0.19061859340124698 1117 58-59 0.21753004746447147 1117 60-61 0.14863708903622808 1117 62-63 0.0443439925305853 1117 64-65 0.23430466203656408 1117 66-67 0.1548354199127857 1117 68-69 0.25340893307600965 1117 70-71 0.21177318293688074 1117 72-73 0.505901085619918 1117 74-75 0.3644392786056656 1117 76 0.5587507011694157 2117 1 0.1635978020758273 2117 2 0.06804749291476497 2117 3 0.057014251934475624 2117 4 0.06078163994640562 2117 5 -0.003472580948383097 2117 6 0.15849974356020624 2117 7 0.17159476699382736 2117 8 0.25391933115304255 2117 9 0.17455346051959708 2117 10-11 0.15310096441019994 2117 12-13 0.23513856205548223 2117 14-15 0.11822227751541448 2117 16-17 0.18742578540306454 2117 18-19 0.22433618874132577 2117 20-21 0.1866358833363364 2117 22-23 0.0945772823069575 2117 24-25 0.26872426411553363 2117 26-27 0.17636388077684728 2117 28-29 0.16719335076581388 2117 30-31 0.24317981671110545 2117 32-33 0.2703639877922299 2117 34-35 0.21715474331359985 2117 36-37 0.15637667599088445 2117 38-39 0.17388561284877113 2117 40-41 0.14225823190789555 2117 42-43 0.1960770205678486 2117 44-45 0.111737057955267 2117 46-47 0.22115736706917488 2117 48-49 0.3067320463969949 2117 50-51 0.16761641441129171 2117 52-53 0.2410468944996822 2117 54-55 0.16393528414510428 2117 56-57 0.2011146816516387 2117 58-59 0.24418598973851147 2117 60-61 0.3507679491250215 2117 62-63 0.323019700223675 2117 64-65 0.3730531502231429 2117 66-67 0.35070096175853394 2117 68-69 0.3843986779837323 2117 70-71 0.30688236377270073 2117 72-73 0.5561463649978506 2117 74-75 0.4567201100085754 2117 76 0.6383676344027123 1116 1 0.15213741801686353 1116 2 0.05860880919850331 1116 3 0.09827641827762079 1116 4 0.001721028832442073 1116 5 -0.08290767590974468 1116 6 0.017169868290245915 1116 7 0.048681777222306266 1116 8 0.22665302190760883 1116 9 0.1475818290961115 1116 10-11 0.13914753900535004 1116 12-13 0.18311893306825766 1116 14-15 0.09055188652290269 1116 16-17 0.14888900621443923 1116 18-19 0.14075178129297683 1116 20-21 0.1008260842846056 1116 22-23 0.184468464867237 1116 24-25 0.2440663798047069 1116 26-27 0.15134606855492905 1116 28-29 0.11827912851387623 1116 30-31 0.18342475138591396 1116 32-33 0.19540555602166876 1116 34-35 0.05980334010660471 1116 36-37 0.1192180896675552 1116 38-39 0.17216442647968222 1116 40-41 0.10230656863815568 1116 42-43 0.19366080593983526 1116 44-45 0.1741099721648922 1116 46-47 0.18000178224994556 1116 48-49 0.27158583214114884 1116 50-51 0.1661471393101266 1116 52-53 0.3078893441411097 1116 54-55 0.1366389330697686 1116 56-57 0.2265853696755542 1116 58-59 0.24147811103900096 1116 60-61 0.274482797433393 1116 62-63 0.31333809865353857 1116 64-65 0.34168321312059646 1116 66-67 0.17028920116666768 1116 68-69 0.2725934156310146 1116 70-71 0.18173254928100135 1116 72-73 0.5182757681709731 1116 74-75 0.3520988633357831 1116 76 0.6559534901734239 2118 1 0.11840046007444727 2118 2 -0.0022431404435678814 2118 3 0.0348961498121696 2118 4 0.06987399554843421 2118 5 -0.05230175074054699 2118 6 0.08770505273817975 2118 7 0.07317352194726823 2118 8 0.12436188261956715 2118 9 0.06148518829446914 2118 10-11 0.04486693664820507 2118 12-13 0.17758878140855927 2118 14-15 0.02767250255121212 2118 16-17 0.19416724616576175 2118 18-19 0.08013635391911578 2118 20-21 0.0011819474829053433 2118 22-23 0.009771934486465739 2118 24-25 0.10240878968399869 2118 26-27 0.08235579047460106 2118 28-29 0.07855775343593763 2118 30-31 0.08593889201854665 2118 32-33 0.09748406939611698 2118 34-35 0.14951950399522218 2118 36-37 0.19865792906902868 2118 38-39 0.1305394649767564 2118 40-41 0.10591670219483262 2118 42-43 0.1677770164005281 2118 44-45 0.14888335585114731 2118 46-47 0.24516038080620461 2118 48-49 0.16539052615512873 2118 50-51 0.016331408842226836 2118 52-53 0.09946134869315415 2118 54-55 0.05227907368738016 2118 56-57 0.18612015600740506 2118 58-59 0.23313044302274477 2118 60-61 0.17281639054372988 2118 62-63 0.18760369654127373 2118 64-65 0.18900644582320325 2118 66-67 0.09022495800036268 2118 68-69 0.18415986158111508 2118 70-71 0.11021916356661166 2118 72-73 0.5179504209461427 2118 74-75 0.25233186453012735 2118 76 0.44886608491378155 1119 1 0.027863096137274113 1119 2 0.022431768598337953 1119 3 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62-63 0.18284814173434683 1119 64-65 0.18092203072490776 1119 66-67 0.1389191666275309 1119 68-69 0.2206124398499938 1119 70-71 0.19267342250145703 1119 72-73 0.4794925536326602 1119 74-75 0.3739184094701642 1119 76 0.16667103782518922 2119 1 0.1425319395581255 2119 2 0.06980322968762209 2119 3 0.09938639145136818 2119 4 0.019524324096259704 2119 5 0.10495214015592325 2119 6 0.14088481856769874 2119 7 0.0789411205681958 2119 8 0.10289339454309498 2119 9 0.0787327232427586 2119 10-11 0.07827777042790274 2119 12-13 0.14034924326155362 2119 14-15 -0.04452838124620939 2119 16-17 0.14425706872730615 2119 18-19 0.1972099018584359 2119 20-21 0.23448240088644923 2119 22-23 0.21580782201824888 2119 24-25 0.19007221487996162 2119 26-27 0.1631048587718169 2119 28-29 0.06406553145680505 2119 30-31 0.08179338041186668 2119 32-33 0.15098724359813076 2119 34-35 0.1467217080192711 2119 36-37 0.20870362777067442 2119 38-39 0.13992509751619053 2119 40-41 0.1247477075285559 2119 42-43 0.15055499011669582 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-0.05511061375824511 2104 60-61 -0.24882416824709708 2104 62-63 -0.1741139904674398 2104 64-65 -0.27623220847599583 2104 66-67 -0.15277878567270875 2104 68-69 -0.4997877794772094 2104 70-71 -0.2844624298463927 2104 72-73 -0.2518685959800777 2104 74-75 -0.09287429989603169 2104 76 -0.4049893541028311 2111 1 -0.08618730179813383 2111 2 -0.021554899367203006 2111 3 -1.2258828452615944E-4 2111 4 -0.059595501287596164 2111 5 0.004666670958783925 2111 6 -0.04548979274931497 2111 7 -0.038426435047263396 2111 8 0.030738211181336794 2111 9 -0.10670996437494296 2111 10-11 -0.0247992334077054 2111 12-13 -0.11081510137081807 2111 14-15 -0.06415146685347395 2111 16-17 -0.1176688569244817 2111 18-19 -0.09259280378807944 2111 20-21 -0.05069263014196679 2111 22-23 -0.1586129030143013 2111 24-25 -0.10510334400157006 2111 26-27 -0.11822823913773561 2111 28-29 -0.12529661161904926 2111 30-31 -0.10769322692229366 2111 32-33 -0.1212643946943146 2111 34-35 -0.15204213205564798 2111 36-37 -0.21447256180767482 2111 38-39 -0.1785757743179559 2111 40-41 -0.14427029479477937 2111 42-43 -0.14295169589289713 2111 44-45 -0.14925013004920373 2111 46-47 -0.10373261237435116 2111 48-49 -0.2826250786860527 2111 50-51 -0.010346188380850663 2111 52-53 -0.08644399320031226 2111 54-55 -0.09234908260678054 2111 56-57 -0.2752509504618459 2111 58-59 -0.12535929710135463 2111 60-61 -0.31656394674200783 2111 62-63 -0.32234784551193485 2111 64-65 -0.3099667986321606 2111 66-67 -0.23459534058045506 2111 68-69 -0.4754276699050237 2111 70-71 -0.2769152899567757 2111 72-73 -0.13213482203475024 2111 74-75 -0.1028975235634988 2111 76 -0.5275008745996388 2110 1 -0.02554640841433553 2110 2 -0.10380889605087162 2110 3 -0.10519454757721292 2110 4 -0.15264101854643286 2110 5 -0.09136291019636644 2110 6 -0.050397043106897854 2110 7 -0.14644132055794046 2110 8 -0.32862867994937517 2110 9 -0.33393551277183775 2110 10-11 -0.168897005011317 2110 12-13 -0.16415959461791374 2110 14-15 -0.1331338156250368 2110 16-17 -0.23785337523391092 2110 18-19 -0.13409716121753945 2110 20-21 -0.20586395274996505 2110 22-23 -0.23852442536154683 2110 24-25 -0.26748641806204887 2110 26-27 -0.21538673311897583 2110 28-29 -0.17136487801399625 2110 30-31 -0.22559974629497503 2110 32-33 -0.2571603416836723 2110 34-35 -0.2869203170612806 2110 36-37 -0.23807451363228438 2110 38-39 -0.4635246053750066 2110 40-41 -0.2741114875342987 2110 42-43 -0.35639414428093374 2110 44-45 -0.384032749063671 2110 46-47 -0.15477220897194854 2110 48-49 -0.29313456883199507 2110 50-51 -0.22144972242386274 2110 52-53 -0.1076251508778725 2110 54-55 -0.28446639144315355 2110 56-57 -0.4357820378889059 2110 58-59 -0.2902861827892451 2110 60-61 -0.3990758450243561 2110 62-63 -0.48930906850346645 2110 64-65 -0.4188131399103412 2110 66-67 -0.3925869674806961 2110 68-69 -0.4277748117857172 2110 70-71 -0.4106657488001062 2110 72-73 0.06979500974379249 2110 74-75 -0.2506035442209438 2110 76 -0.3916658430185649 2109 1 -0.0021177080119798575 2109 2 -0.0859404552931693 2109 3 -0.024083040655618504 2109 4 -0.15643158407448254 2109 5 -0.1309349846522636 2109 6 -0.2980488710931013 2109 7 -0.09016319146545015 2109 8 -0.7966007115945573 2109 9 -0.24198309902121906 2109 10-11 -0.38019205500191333 2109 12-13 -0.49441083905133354 2109 14-15 -0.1966254920202175 2109 16-17 -0.29563323650189943 2109 18-19 -0.4431409557166006 2109 20-21 -0.4110678052409682 2109 22-23 -0.18648116125731917 2109 24-25 -0.31557719416829855 2109 26-27 -0.1270015503677442 2109 28-29 -0.19795091848039448 2109 30-31 -0.1680347778659126 2109 32-33 -0.469364971402527 2109 34-35 -0.24718755430060924 2109 36-37 -0.15400584823677832 2109 38-39 -0.027439551055941536 2109 40-41 -0.3230887397968658 2109 42-43 -0.2760664309737493 2109 44-45 -0.4076295427618106 2109 46-47 -0.07225864105377156 2109 48-49 -0.7341855582177814 2109 50-51 -0.4730257019758213 2109 52-53 -0.5797913694273902 2109 54-55 -0.3957720453808733 2109 56-57 -0.6082301595092687 2109 58-59 -0.3371887540390759 2109 60-61 -0.5468213115901293 2109 62-63 -0.48238095892065047 2109 64-65 -0.6950789391991492 2109 66-67 -0.37962546869472646 2109 68-69 -1.023500858318279 2109 70-71 -0.36129548898168196 2109 72-73 -0.024750236923374302 2109 74-75 -0.2261873635849767 2109 76 -1.3118260198852951 2108 1 0.14058710665133844 2108 2 0.020151074328694563 2108 3 -0.004894947905754066 2108 4 -0.019478423072278872 2108 5 0.04132837764567654 2108 6 0.13543357394230782 2108 7 0.1014753865296214 2108 8 -0.3446139311733205 2108 9 -0.022368831230281216 2108 10-11 -0.028543734475881877 2108 12-13 -0.29100642529640197 2108 14-15 0.025757891947577605 2108 16-17 -0.10562773609872522 2108 18-19 -0.12001431940228713 2108 20-21 -0.19650772825555407 2108 22-23 0.11752140869936056 2108 24-25 0.10041001390473525 2108 26-27 0.08181921295631867 2108 28-29 0.09559835675702999 2108 30-31 0.014981174719366663 2108 32-33 -0.029440743046983187 2108 34-35 0.09179416105646254 2108 36-37 -0.08399855145330548 2108 38-39 -0.12191388122658964 2108 40-41 -0.04009653665376334 2108 42-43 0.1343508855658584 2108 44-45 0.07626685869196592 2108 46-47 0.09047126224513846 2108 48-49 -0.24178568315522142 2108 50-51 -0.06116470661120843 2108 52-53 -0.14902544384852234 2108 54-55 0.054196234750882866 2108 56-57 -0.16211924192711535 2108 58-59 0.07688691726702501 2108 60-61 -0.15662121343655144 2108 62-63 0.057442546715492426 2108 64-65 -0.025678709715606374 2108 66-67 0.05952801541317854 2108 68-69 -0.15952578835452869 2108 70-71 -0.07137397138018287 2108 72-73 0.34420720909317737 2108 74-75 0.032725302393082245 2108 76 -0.1569214970933146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 9.0 13 58.0 14 250.0 15 483.0 16 830.0 17 1170.0 18 1543.0 19 1638.0 20 1698.0 21 1820.0 22 1974.0 23 2165.0 24 2624.0 25 3068.0 26 3773.0 27 4538.0 28 5640.0 29 6679.0 30 8826.0 31 11066.0 32 14825.0 33 20214.0 34 29572.0 35 47535.0 36 105049.0 37 933515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25579170752218 25.850283379606413 8.049368601655257 24.84455631121615 2 15.24062691284332 22.730175198894404 41.46338038291148 20.565817505350797 3 18.690663135538966 25.170106239472904 32.84799602169892 23.2912346032892 4 13.538779761880177 15.96152877122869 41.696081257790766 28.803610209100363 5 12.20915488362065 36.412193624970776 38.19681190714764 13.18183958426093 6 27.68050012927826 38.99123303377674 18.293246057425982 15.035020779519018 7 23.808099840818393 34.70638138987764 23.3681407142273 18.11737805507667 8 20.86129887895507 41.358966500821516 22.303763443080804 15.475971177142611 9 21.139433009490595 17.515924356107508 25.592552911156506 35.75208972324539 10-11 23.704470714962493 26.736025350104537 30.617512289695654 18.941991645237312 12-13 20.700589062811062 24.76277645103493 31.73673989130713 22.79989459484688 14-15 20.013349116570993 28.719517383176647 25.800719827981432 25.466413672270928 16-17 19.055987813974774 34.081880639402854 28.05189634278813 18.810235203834235 18-19 18.561632689475264 32.23836433671164 31.039752446580813 18.16025052723228 20-21 18.76616494584769 29.9883030018165 31.756524008997438 19.48900804333837 22-23 19.223016982950963 30.301172921449204 31.321025602052593 19.15478449354724 24-25 19.153751919555052 30.82931386697308 31.100096070684234 18.916838142787636 26-27 18.81114386894736 31.113684744421416 31.06465813127237 19.010513255358852 28-29 18.6255283681118 31.041734988645814 31.416063712293496 18.916672930948884 30-31 18.711892856818334 30.708544012846872 31.5450941583572 19.034468971977596 32-33 18.853727220385156 30.771613632289665 31.282902970261045 19.091756177064138 34-35 18.821676123667668 30.7018529333775 31.576195287001873 18.900275655952957 36-37 18.798422557363615 30.97056998910428 31.6124179826478 18.618589470884306 38-39 18.7060691395024 30.813949165969333 31.48037242052691 18.999609274001354 40-41 18.80197461189674 30.766822488965918 31.437458645411613 18.993744253725733 42-43 18.9836250286023 30.757818443754044 31.408711785469123 18.849844742174536 44-45 18.58562970905369 30.628416271053148 31.67234857715434 19.113605442738816 46-47 18.740639716760825 30.53891275741037 31.888280450400515 18.83216707542829 48-49 18.555726366239952 30.798212738328402 31.716831864737767 18.929229030693882 50-51 18.492202414240598 30.851452253365576 31.71344502204339 18.94290031035044 52-53 18.399848996379383 30.630770539755336 31.927889988740816 19.04149047512447 54-55 18.45672317186905 30.378698576782615 32.11239030966481 19.052187941683524 56-57 18.261360172547246 30.58190913844504 32.025364973603274 19.13136571540443 58-59 18.1426967693651 30.699126938038123 32.11239030966481 19.045785982931964 60-61 18.34714641981815 30.39141988836636 32.12853976690261 19.13289392491287 62-63 18.105648014525425 30.00238731106994 32.36648611766222 19.525478556742417 64-65 18.249630131995996 29.818795655259066 32.265252563468195 19.666321649276743 66-67 18.25223221845631 29.946752224370893 32.03759064967077 19.76342490750202 68-69 18.129149398587604 30.064837386117414 31.94792192418924 19.858091291105737 70-71 18.256238605545995 29.917303214113716 32.04192746043796 19.784530719902328 72-73 18.14034250066291 29.402750942327028 32.309942365850056 20.14696419116001 74-75 18.423432986360936 29.557843555953532 31.808524435243978 20.21019902244155 76 18.391960131079074 29.4028335482464 31.745124392123692 20.460081928550835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 6.0 2 3.0 3 3.5 4 2.0 5 3.5 6 6.5 7 20.5 8 33.0 9 44.5 10 109.5 11 254.5 12 346.0 13 489.5 14 866.5 15 1457.5 16 2420.5 17 3026.0 18 4025.0 19 6255.0 20 9095.0 21 10704.0 22 12757.0 23 17434.0 24 22760.0 25 25462.0 26 28405.5 27 34160.5 28 39658.0 29 42344.0 30 45334.5 31 50479.5 32 53769.0 33 54904.0 34 56594.0 35 59804.0 36 62254.0 37 63184.0 38 63639.5 39 64740.0 40 64818.5 41 63302.0 42 62352.0 43 61620.5 44 58977.0 45 54707.0 46 52349.0 47 49152.5 48 42846.0 49 36700.0 50 33664.0 51 31036.5 52 25424.5 53 20236.0 54 18032.0 55 16153.0 56 12705.0 57 9645.0 58 8154.0 59 7267.5 60 5573.5 61 4060.5 62 3355.0 63 2942.5 64 2082.0 65 1359.5 66 930.0 67 775.0 68 635.5 69 422.0 70 307.5 71 267.0 72 202.0 73 118.0 74 79.5 75 60.0 76 52.5 77 41.5 78 31.5 79 25.0 80 19.5 81 15.5 82 13.0 83 9.0 84 12.5 85 14.0 86 12.5 87 13.0 88 14.5 89 16.5 90 12.5 91 9.0 92 10.0 93 9.0 94 11.0 95 9.5 96 5.0 97 9.0 98 13.0 99 49.0 100 85.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1210567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.1167023070216 #Duplication Level Percentage of deduplicated Percentage of total 1 95.0519311305224 86.6081851252733 2 3.841627004774205 7.000727683372525 3 0.5093318005863824 1.3922590214858608 4 0.15883170916563724 0.5788888624384321 5 0.11640117161301265 0.5303045451025703 6 0.06719886006936374 0.3673763116986849 7 0.04184772973776266 0.266911899291829 8 0.033239015108520384 0.2422903555697316 9 0.026523035503770737 0.21750223772480787 >10 0.14794609933625455 2.4294325071780865 >50 0.004291966238873263 0.24378014767050205 >100 8.304773437971792E-4 0.12234130319364793 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.478177581249117E-4 0.0 0.0 2 2.478177581249117E-4 0.0 0.0 3 2.478177581249117E-4 0.0 0.0 4 2.478177581249117E-4 0.0 0.0 5 3.304236774998823E-4 8.260591937497058E-5 0.0 6 3.304236774998823E-4 8.260591937497058E-5 0.0 7 3.304236774998823E-4 8.260591937497058E-5 0.0 8 3.304236774998823E-4 8.260591937497058E-5 0.0 9 3.304236774998823E-4 8.260591937497058E-5 0.0 10 6.608473549997646E-4 8.260591937497058E-5 0.0 11 6.608473549997646E-4 1.6521183874994115E-4 0.0 12 6.608473549997646E-4 1.6521183874994115E-4 0.0 13 6.608473549997646E-4 1.6521183874994115E-4 0.0 14 6.608473549997646E-4 1.6521183874994115E-4 0.0 15 6.608473549997646E-4 1.6521183874994115E-4 0.0 16 6.608473549997646E-4 1.6521183874994115E-4 0.0 17 6.608473549997646E-4 1.6521183874994115E-4 0.0 18 6.608473549997646E-4 1.6521183874994115E-4 0.0 19 6.608473549997646E-4 2.478177581249117E-4 0.0 20 6.608473549997646E-4 2.478177581249117E-4 0.0 21 6.608473549997646E-4 2.478177581249117E-4 1.6521183874994115E-4 22 7.434532743747351E-4 2.478177581249117E-4 4.956355162498234E-4 23 7.434532743747351E-4 2.478177581249117E-4 6.608473549997646E-4 24 8.260591937497057E-4 2.478177581249117E-4 8.260591937497057E-4 25 8.260591937497057E-4 2.478177581249117E-4 0.0010738769518746174 26 8.260591937497057E-4 2.478177581249117E-4 0.0014043006293744998 27 8.260591937497057E-4 2.478177581249117E-4 0.003717266371873676 28 9.086651131246763E-4 2.478177581249117E-4 0.010738769518746175 29 9.086651131246763E-4 2.478177581249117E-4 0.01619076019749423 30 9.086651131246763E-4 2.478177581249117E-4 0.02296444558624182 31 9.086651131246763E-4 2.478177581249117E-4 0.028416436264989877 32 0.0010738769518746174 2.478177581249117E-4 0.035272727573112436 33 0.0010738769518746174 2.478177581249117E-4 0.04254204847810984 34 0.0010738769518746174 3.304236774998823E-4 0.055098148223105374 35 0.0010738769518746174 4.1302959687485285E-4 0.07228017945309925 36 0.0010738769518746174 4.1302959687485285E-4 0.10730508926808677 37 0.001156482871249588 4.1302959687485285E-4 0.16892910512181483 38 0.001156482871249588 4.956355162498234E-4 0.24013540762303945 39 0.001156482871249588 4.956355162498234E-4 0.3241456276273845 40 0.001156482871249588 4.956355162498234E-4 0.41418607974610244 41 0.001156482871249588 4.956355162498234E-4 0.5145522717866917 42 0.001156482871249588 4.956355162498234E-4 0.61756185324728 43 0.001156482871249588 4.956355162498234E-4 0.7344492291628634 44 0.0012390887906245586 4.956355162498234E-4 0.8600102266128187 45 0.0012390887906245586 4.956355162498234E-4 1.0158049905540132 46 0.0012390887906245586 4.956355162498234E-4 1.2008422499539473 47 0.0013216947099995292 4.956355162498234E-4 1.406200565520124 48 0.0013216947099995292 4.956355162498234E-4 1.611228457408801 49 0.0013216947099995292 4.956355162498234E-4 1.8432684849330934 50 0.0013216947099995292 4.956355162498234E-4 2.088442853638006 51 0.0013216947099995292 4.956355162498234E-4 2.3232088765016723 52 0.0013216947099995292 4.956355162498234E-4 2.585234852759079 53 0.0013216947099995292 4.956355162498234E-4 2.847013011258361 54 0.0013216947099995292 4.956355162498234E-4 3.116143096582015 55 0.0014043006293744998 4.956355162498234E-4 3.3974988579731646 56 0.0014869065487494702 4.956355162498234E-4 3.713053469985552 57 0.0015695124681244408 5.78241435624794E-4 4.050994286148557 58 0.0015695124681244408 5.78241435624794E-4 4.4194992924803005 59 0.0015695124681244408 5.78241435624794E-4 4.7981648268951655 60 0.0015695124681244408 5.78241435624794E-4 5.20838582251127 61 0.0016521183874994114 5.78241435624794E-4 5.601755210574879 62 0.001734724306874382 5.78241435624794E-4 6.011810994352233 63 0.001734724306874382 6.608473549997646E-4 6.433762030519583 64 0.0018173302262493526 6.608473549997646E-4 6.850013258250059 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGGAC 190 0.0 25.789473 3 GTATATG 390 0.0 23.333334 1 GTCCTAT 155 3.5091944E-8 22.580645 1 TAGACTG 265 0.0 22.452831 5 CCCAGGG 95 2.7815253E-4 22.105263 2 ACTGTCC 255 0.0 21.960785 8 GTAGGGC 130 4.3452255E-6 21.538462 3 CTAAGAC 215 1.1459633E-10 21.16279 3 AACCGCG 150 6.385926E-7 21.0 7 GGACCGC 85 0.0037908799 20.588236 6 CTACACT 460 0.0 20.543478 4 CCAGGGG 155 8.75003E-7 20.32258 3 ACCGCGC 140 8.251842E-6 20.000002 8 GTGTAAG 295 1.8189894E-12 18.983051 1 TACACTC 370 0.0 18.918919 5 CTATAGA 300 1.8189894E-12 18.666666 1 TACACTG 545 0.0 18.623852 5 GTGTTAC 290 1.8189894E-11 18.103449 1 GTACAGG 155 1.9847454E-5 18.064516 1 TAGCCAG 155 1.9847454E-5 18.064516 5 >>END_MODULE