FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve15.3420000000c843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve15.3420000000c843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210567
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAC1900.025.7894733
GTATATG3900.023.3333341
GTCCTAT1553.5091944E-822.5806451
TAGACTG2650.022.4528315
CCCAGGG952.7815253E-422.1052632
ACTGTCC2550.021.9607858
GTAGGGC1304.3452255E-621.5384623
CTAAGAC2151.1459633E-1021.162793
AACCGCG1506.385926E-721.07
GGACCGC850.003790879920.5882366
CTACACT4600.020.5434784
CCAGGGG1558.75003E-720.322583
ACCGCGC1408.251842E-620.0000028
GTGTAAG2951.8189894E-1218.9830511
TACACTC3700.018.9189195
CTATAGA3001.8189894E-1218.6666661
TACACTG5450.018.6238525
GTGTTAC2901.8189894E-1118.1034491
GTACAGG1551.9847454E-518.0645161
TAGCCAG1551.9847454E-518.0645165
Produced by FastQC (version 0.11.2)