FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve14.3420000000c829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve14.3420000000c829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362122
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG4500.018.6666665
TACACCG2659.458745E-1118.4905665
GTCCTAA2701.2369128E-1018.1481481
TAGGGTG1200.001341241317.55
ACCGCTC2903.4197E-1016.8965518
GTCTTAA2903.4197E-1016.8965511
GTGCAGG2552.1771484E-816.4705891
TACACTG5750.016.4347825
CTACACC5400.016.2037034
GCCCTAT1957.837747E-616.1538471
CTATACT3753.6379788E-1215.8666674
AGACAGC3602.7284841E-1115.5555566
GCCTTAC1350.0029469815.5555551
GCTACAC3401.9645086E-1015.4411763
GTGTAAC2957.8034645E-915.4237291
CTCTTAG1605.162991E-415.3125011
TCTAGCC2302.216675E-615.2173923
GTAGGGC1859.036293E-515.1351363
TGCACCG2553.9025326E-715.098045
CCTTATA2806.874325E-815.02