##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve13.3420000000c80f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454398 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.42798601208197 34.0 34.0 34.0 34.0 34.0 2 33.60974918832397 34.0 34.0 34.0 34.0 34.0 3 33.63472859561138 34.0 34.0 34.0 34.0 34.0 4 33.68438694222627 34.0 34.0 34.0 34.0 34.0 5 33.66747203997805 34.0 34.0 34.0 34.0 34.0 6 37.43455024003058 38.0 38.0 38.0 37.0 38.0 7 37.4527364586585 38.0 38.0 38.0 37.0 38.0 8 37.39453024550364 38.0 38.0 38.0 37.0 38.0 9 37.42704404158972 38.0 38.0 38.0 37.0 38.0 10-11 37.40020853989073 38.0 38.0 38.0 37.0 38.0 12-13 37.429908800754674 38.0 38.0 38.0 37.0 38.0 14-15 37.400763064855695 38.0 38.0 38.0 37.0 38.0 16-17 37.410300344197395 38.0 38.0 38.0 37.0 38.0 18-19 37.39210003039058 38.0 38.0 38.0 37.0 38.0 20-21 37.39269065276493 38.0 38.0 38.0 37.0 38.0 22-23 37.34827124349731 38.0 38.0 38.0 37.0 38.0 24-25 37.379460092766905 38.0 38.0 38.0 37.0 38.0 26-27 37.3658723403085 38.0 38.0 38.0 37.0 38.0 28-29 37.40096967955127 38.0 38.0 38.0 37.0 38.0 30-31 37.36215946391566 38.0 38.0 38.0 37.0 38.0 32-33 37.35458622742881 38.0 38.0 38.0 37.0 38.0 34-35 37.34647221737104 38.0 38.0 38.0 37.0 38.0 36-37 37.30963738949036 38.0 38.0 38.0 37.0 38.0 38-39 37.29998219194471 38.0 38.0 38.0 37.0 38.0 40-41 37.29914438826236 38.0 38.0 38.0 37.0 38.0 42-43 37.30022215377084 38.0 38.0 38.0 37.0 38.0 44-45 37.31288409362499 38.0 38.0 38.0 37.0 38.0 46-47 37.2710867314174 38.0 38.0 38.0 37.0 38.0 48-49 37.26644872999 38.0 38.0 38.0 37.0 38.0 50-51 37.253879612045665 38.0 38.0 38.0 37.0 38.0 52-53 37.24277948677047 38.0 38.0 38.0 37.0 38.0 54-55 37.221572774440006 38.0 38.0 38.0 37.0 38.0 56-57 37.220002021454924 38.0 38.0 38.0 37.0 38.0 58-59 37.19355705934689 38.0 38.0 38.0 37.0 38.0 60-61 37.20473453621361 38.0 38.0 38.0 37.0 38.0 62-63 37.14591294817512 38.0 38.0 38.0 37.0 38.0 64-65 37.12017721421509 38.0 38.0 38.0 37.0 38.0 66-67 37.11563787903999 38.0 38.0 38.0 37.0 38.0 68-69 37.0824141672362 38.0 38.0 38.0 37.0 38.0 70-71 37.074238275905216 38.0 38.0 38.0 37.0 38.0 72-73 36.81179876484978 38.0 38.0 38.0 36.0 38.0 74-75 36.958149694925325 38.0 38.0 38.0 36.0 38.0 76 36.10463023188976 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0021792938383029536 1101 2 0.047750375926426614 1101 3 0.02706356092554074 1101 4 0.030174522326504416 1101 5 0.03910841041977875 1101 6 0.02860591035435789 1101 7 0.014852259171682647 1101 8 0.09990753598064828 1101 9 0.06960882803878121 1101 10-11 0.059873647654491435 1101 12-13 0.028253575890126115 1101 14-15 0.026894747023490595 1101 16-17 0.05458875799262586 1101 18-19 -0.03159772742817779 1101 20-21 0.024460764970399396 1101 22-23 0.03883855855017515 1101 24-25 -0.015774653628433555 1101 26-27 0.04947463731557633 1101 28-29 -0.012920073539177679 1101 30-31 0.07128138612773682 1101 32-33 0.097155542342513 1101 34-35 0.039280655559032596 1101 36-37 0.016663317181262016 1101 38-39 0.06062765118645075 1101 40-41 -0.006924044390039796 1101 42-43 -0.010314363065063503 1101 44-45 0.0105022080389503 1101 46-47 0.0295177947666474 1101 48-49 -0.001499669241667334 1101 50-51 0.03965782078036284 1101 52-53 0.008121816988030162 1101 54-55 0.0337042321867429 1101 56-57 0.006349277247196028 1101 58-59 0.03964419713881284 1101 60-61 -0.078846337910079 1101 62-63 0.020370582689992034 1101 64-65 -0.019005029021911923 1101 66-67 0.027997472554780245 1101 68-69 0.01668166578256347 1101 70-71 0.029463538681696377 1101 72-73 0.30906316801398503 1101 74-75 0.13784391558367304 1101 76 -0.05660130481597747 1102 1 0.011320257976919379 1102 2 0.029813988446129258 1102 3 -0.0010390838777070144 1102 4 -0.012052962900909847 1102 5 0.03228938541737847 1102 6 0.004784913765483623 1102 7 0.0020563468468282053 1102 8 0.07444818995482905 1102 9 0.022136529319034537 1102 10-11 0.09092130749422012 1102 12-13 0.007372934744758197 1102 14-15 -0.04024386636569943 1102 16-17 0.0010841136575336918 1102 18-19 0.018871237763924853 1102 20-21 0.01946309263504986 1102 22-23 0.0047991963796292225 1102 24-25 -0.08201320420143077 1102 26-27 -0.018160024769215966 1102 28-29 -0.06617093755867387 1102 30-31 -0.013444663000939272 1102 32-33 -0.09286208066450286 1102 34-35 -0.05943434442604456 1102 36-37 -0.11994172652054402 1102 38-39 0.07377330707836904 1102 40-41 0.06260695021743601 1102 42-43 0.05857628773876655 1102 44-45 0.06609447184768413 1102 46-47 0.09369296793143178 1102 48-49 0.09354083053956685 1102 50-51 0.1171907374547203 1102 52-53 0.1390058550680564 1102 54-55 0.11323477423506034 1102 56-57 0.11786427050783743 1102 58-59 0.15141948805425187 1102 60-61 0.11999107232347228 1102 62-63 0.18567122724266483 1102 64-65 0.13767031301505028 1102 66-67 0.08823364509079568 1102 68-69 0.16631209914968537 1102 70-71 0.06831574266612961 1102 72-73 0.3616494157095431 1102 74-75 0.2175556335707256 1102 76 0.41337657216747914 1103 1 -0.09145202801244068 1103 2 -0.028746666276092014 1103 3 -0.02660754882324312 1103 4 0.02470302521668799 1103 5 0.025764438932434075 1103 6 0.04302794155059786 1103 7 0.031216324449083288 1103 8 0.043454038086494506 1103 9 0.04728114584912646 1103 10-11 0.014268031670816583 1103 12-13 -0.023251227056100277 1103 14-15 0.014115348980730857 1103 16-17 -0.06561760416439455 1103 18-19 0.036229249307623945 1103 20-21 0.006614870952255103 1103 22-23 0.0014272060771816086 1103 24-25 0.007012753081120593 1103 26-27 0.04255331608089108 1103 28-29 -0.0638204249889256 1103 30-31 0.0013329691541628108 1103 32-33 -0.10970567632714534 1103 34-35 -0.025003785840212345 1103 36-37 -0.030304072669522952 1103 38-39 0.0034056818958561053 1103 40-41 -0.008821241957697623 1103 42-43 0.04350742381424055 1103 44-45 0.022179595500652738 1103 46-47 0.046792233596512744 1103 48-49 -0.023303497474557844 1103 50-51 -0.02418117045818491 1103 52-53 0.012670709673400893 1103 54-55 -0.0158570641654876 1103 56-57 0.0011452966543146204 1103 58-59 -0.060021001522706285 1103 60-61 -0.074788543283546 1103 62-63 -0.06593303443262499 1103 64-65 -0.01536389542742711 1103 66-67 -0.013060603051243902 1103 68-69 -0.0037524850959158584 1103 70-71 -0.05084598952819164 1103 72-73 0.22379340516368273 1103 74-75 -0.021965439080062765 1103 76 0.23578835814386423 2116 1 -0.026381063815023253 2116 2 0.022262093605483813 2116 3 0.04190449219863979 2116 4 -0.01959755775801142 2116 5 0.042661548476736755 2116 6 0.029771829472949207 2116 7 0.05366023984144164 2116 8 0.0029229511896886606 2116 9 0.03866746578319891 2116 10-11 -0.007589093745046682 2116 12-13 -0.016897871136315246 2116 14-15 -0.008437683403364815 2116 16-17 0.0164830886903502 2116 18-19 0.03402355996293238 2116 20-21 0.027897110063449304 2116 22-23 0.005631796621393903 2116 24-25 -0.04748805575811588 2116 26-27 0.04374154670689734 2116 28-29 -0.08685952397253516 2116 30-31 -0.02057207617134793 2116 32-33 0.021752981304935304 2116 34-35 0.04187943608696543 2116 36-37 -0.015043870612316823 2116 38-39 0.029834427737199576 2116 40-41 0.021401265138727865 2116 42-43 -0.02926964177803626 2116 44-45 -0.015403348674176698 2116 46-47 -0.05435097673220923 2116 48-49 -0.03696570789484355 2116 50-51 -0.14055253449689076 2116 52-53 -0.08092313093948889 2116 54-55 -0.09936864735383466 2116 56-57 -0.12968902865167564 2116 58-59 -0.30346986151720756 2116 60-61 -0.09385761510237245 2116 62-63 -0.06449558975417347 2116 64-65 -0.1464686477394963 2116 66-67 -0.13833133527967334 2116 68-69 -0.03487173546980671 2116 70-71 -0.09321878811196171 2116 72-73 0.16057498804681103 2116 74-75 -0.194091923223759 2116 76 0.139876811582198 1117 1 -0.0657072390864073 1117 2 0.03493595779963243 1117 3 6.388323965111908E-4 1117 4 0.06288023671039866 1117 5 -0.07084627186626591 1117 6 -0.04896228873127484 1117 7 0.027881967100043425 1117 8 0.08211030643132489 1117 9 -0.0013901785681724732 1117 10-11 -0.23303665761138603 1117 12-13 0.03535813934178833 1117 14-15 0.046347328078645944 1117 16-17 0.048989310679068865 1117 18-19 -0.11656762146262167 1117 20-21 -0.11421607216620089 1117 22-23 -0.3749756396725985 1117 24-25 -0.26490469097569047 1117 26-27 -0.14507527646529184 1117 28-29 -0.11173724246978622 1117 30-31 -0.16999433769467487 1117 32-33 0.0895169132564817 1117 34-35 0.13251950544763957 1117 36-37 -0.001811322396534365 1117 38-39 0.025866969539833917 1117 40-41 0.053784218986244525 1117 42-43 0.04180094726978467 1117 44-45 0.07627694593606549 1117 46-47 0.05099999522998644 1117 48-49 0.08009801908256975 1117 50-51 0.03331949109651333 1117 52-53 0.07108042342552068 1117 54-55 0.11252947235993815 1117 56-57 0.1082300151970017 1117 58-59 0.12960922742084335 1117 60-61 0.17049489108261895 1117 62-63 -3.183968885593913E-4 1117 64-65 0.16719487648371256 1117 66-67 0.11842349986537215 1117 68-69 0.1609494807100731 1117 70-71 0.20497776013342417 1117 72-73 0.3951520408097693 1117 74-75 0.35357480017461995 1117 76 0.37894362427135064 2117 1 0.09627171144664715 2117 2 0.07557516658588526 2117 3 0.03261689500995146 2117 4 0.026408355208737078 2117 5 -0.005891820423130412 2117 6 0.072865563182404 2117 7 0.07211768016028941 2117 8 0.13550921481828482 2117 9 0.11694211926830889 2117 10-11 0.09121583504178687 2117 12-13 0.14400861328577008 2117 14-15 0.07759767732088818 2117 16-17 0.10612199472988237 2117 18-19 0.16127961927499257 2117 20-21 0.08721736536361391 2117 22-23 0.016056851185084042 2117 24-25 0.13159651889154844 2117 26-27 0.050205326396515204 2117 28-29 0.13650180906995502 2117 30-31 0.1159670821366916 2117 32-33 0.17056601300055974 2117 34-35 0.13984671512402969 2117 36-37 0.022111755764093743 2117 38-39 0.10749852858544529 2117 40-41 0.10567102360426617 2117 42-43 0.13047378254202613 2117 44-45 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0.13413867351984265 1119 50-51 0.13916976377871038 1119 52-53 0.09797680418903809 1119 54-55 0.08774018133366468 1119 56-57 0.12992064484499366 1119 58-59 0.2083152264292707 1119 60-61 0.11285978944652442 1119 62-63 0.16924035373116908 1119 64-65 0.1426534783677056 1119 66-67 0.21138334121391011 1119 68-69 0.23035949558364877 1119 70-71 0.1969212405498908 1119 72-73 0.4856329530556778 1119 74-75 0.3176303281865174 1119 76 0.15782314831696453 2119 1 0.10438230489560141 2119 2 0.06016225655164931 2119 3 0.06043017016001784 2119 4 0.04972147596502907 2119 5 0.06521007607535978 2119 6 0.030360108501220395 2119 7 0.023646857190563253 2119 8 0.0903414006608898 2119 9 0.10500731076749048 2119 10-11 0.08106554712284009 2119 12-13 0.11673486803210409 2119 14-15 -0.012930010063961106 2119 16-17 0.13879850414112127 2119 18-19 0.11637410487225708 2119 20-21 0.17212274403555483 2119 22-23 0.19024933780712416 2119 24-25 0.12751271870084224 2119 26-27 0.1258165391172028 2119 28-29 0.08588168901606963 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-0.11937860400913536 2104 44-45 -0.08618815702678262 2104 46-47 0.007533614396649568 2104 48-49 -0.0552450856539366 2104 50-51 0.05443975756393371 2104 52-53 0.06747326735075632 2104 54-55 0.0347642094887064 2104 56-57 -0.1922393054367646 2104 58-59 0.03336532872874187 2104 60-61 -0.08180660255945327 2104 62-63 -0.035414586320641206 2104 64-65 -0.1397637917378063 2104 66-67 -0.049559350134977365 2104 68-69 -0.3822783642974983 2104 70-71 -0.23174376884211512 2104 72-73 -0.009714760394082589 2104 74-75 0.08481845446526393 2104 76 -0.26321967780187094 2111 1 -0.008576811037769971 2111 2 -0.054592654333532664 2111 3 -0.019428918568536346 2111 4 0.022332314835665557 2111 5 0.05464858383911775 2111 6 0.05071339734232794 2111 7 -0.03397592618777878 2111 8 0.06227267284268123 2111 9 -0.0014331705570569397 2111 10-11 0.048815445537421454 2111 12-13 0.01987027534639907 2111 14-15 -0.004629113689397002 2111 16-17 -0.011328036340408687 2111 18-19 0.056925102832238395 2111 20-21 0.07691425336307134 2111 22-23 -0.0511198122049592 2111 24-25 0.07144258287749494 2111 26-27 -0.029514601678464203 2111 28-29 -0.025763392622614845 2111 30-31 0.03397584846339896 2111 32-33 0.0015871346073481618 2111 34-35 0.029234697025181333 2111 36-37 -0.052374172963823185 2111 38-39 0.019352305275575077 2111 40-41 -0.05916048973338661 2111 42-43 7.453505873655786E-5 2111 44-45 -0.04126625205609713 2111 46-47 0.043024223420438545 2111 48-49 -0.16205904332994692 2111 50-51 0.03766760715173234 2111 52-53 0.022290027835843773 2111 54-55 0.07957321918893712 2111 56-57 -0.055282780293687495 2111 58-59 -0.011349653773272905 2111 60-61 -0.23844111782223365 2111 62-63 -0.1367761408618975 2111 64-65 -0.07548621969074532 2111 66-67 -0.1045641650164768 2111 68-69 -0.2710318188771197 2111 70-71 -0.10456035708754285 2111 72-73 0.024372799987659732 2111 74-75 0.06310748827904433 2111 76 -0.1473246187314956 2110 1 0.15494758704831213 2110 2 0.05565813859064406 2110 3 0.01704559341524714 2110 4 -0.039644382458043026 2110 5 -0.01786773786466256 2110 6 0.02993278937929489 2110 7 0.02415440765782506 2110 8 -0.1725233467324685 2110 9 -0.04763421906984888 2110 10-11 0.020521404185465997 2110 12-13 -0.022446807128623902 2110 14-15 0.02509110124482561 2110 16-17 0.01163025203329937 2110 18-19 -0.0031278677723420856 2110 20-21 -0.09607128326403114 2110 22-23 -0.06466273910334763 2110 24-25 -0.0421575653530013 2110 26-27 -0.05001237277134152 2110 28-29 -0.03656895998575038 2110 30-31 -0.037294138130462784 2110 32-33 -0.0989125983597674 2110 34-35 -0.1098796257467285 2110 36-37 -0.07682948037862047 2110 38-39 -0.1340886041621303 2110 40-41 -0.005665083788905179 2110 42-43 -0.09896632642204395 2110 44-45 -0.046420531666775844 2110 46-47 -0.01390718727613205 2110 48-49 -0.0785780244632619 2110 50-51 -0.02348346806434165 2110 52-53 0.017115039552592748 2110 54-55 -0.04145861767032244 2110 56-57 -0.16521204382789278 2110 58-59 -0.09745685930139558 2110 60-61 -0.17117062986051224 2110 62-63 -0.19386450149798407 2110 64-65 -0.20886151533429143 2110 66-67 -0.18020913117414494 2110 68-69 -0.25604527951429645 2110 70-71 -0.22043355461557468 2110 72-73 0.20871976834698813 2110 74-75 -0.08657697694674482 2110 76 -0.06464469851437826 2109 1 0.1163797823040369 2109 2 -0.029109544706450663 2109 3 0.03390863233710917 2109 4 0.011857057278696459 2109 5 -0.009377208785899427 2109 6 -0.12880504141735827 2109 7 0.015070844162494268 2109 8 -0.49636470145617295 2109 9 -0.0924844668612721 2109 10-11 -0.1456631465579008 2109 12-13 -0.30976830915600573 2109 14-15 -0.09879751014378968 2109 16-17 -0.16867547165477248 2109 18-19 -0.2281085243706471 2109 20-21 -0.3071983914499583 2109 22-23 -0.07447988274402206 2109 24-25 -0.16244561064105056 2109 26-27 -0.030455214243595208 2109 28-29 -0.127802971072704 2109 30-31 -0.12173322845190171 2109 32-33 -0.2531909180792624 2109 34-35 -0.08398468735179421 2109 36-37 -0.08554625380126879 2109 38-39 0.01124424925895795 2109 40-41 -0.15752621285592028 2109 42-43 -0.12333950226580725 2109 44-45 -0.16566819576738823 2109 46-47 -0.024748551793969398 2109 48-49 -0.3878902808343412 2109 50-51 -0.19920333643126753 2109 52-53 -0.33825361140429067 2109 54-55 -0.22887757126574826 2109 56-57 -0.448933714314272 2109 58-59 -0.19266679642310436 2109 60-61 -0.3768460818594974 2109 62-63 -0.40931302356415245 2109 64-65 -0.46855011105229494 2109 66-67 -0.19003827262093864 2109 68-69 -0.6030818214037765 2109 70-71 -0.2008367298716962 2109 72-73 0.16147216301114753 2109 74-75 -0.11107257938351722 2109 76 -1.0106511430502394 2108 1 0.2269220178462703 2108 2 0.09386470326779772 2108 3 0.06022953365800987 2108 4 0.07149186691350451 2108 5 0.08833063892194559 2108 6 0.1993251617128493 2108 7 0.16490711899876942 2108 8 -0.14042897052043912 2108 9 0.15324202886522187 2108 10-11 0.11372330032853739 2108 12-13 -0.042577166964861135 2108 14-15 0.15277730393102473 2108 16-17 0.10758299210369415 2108 18-19 0.09432598556386296 2108 20-21 0.048168463916894666 2108 22-23 0.2081125248483886 2108 24-25 0.1632148400193998 2108 26-27 0.16270579141741592 2108 28-29 0.16498063421090592 2108 30-31 0.14547178875311317 2108 32-33 0.0567560793677373 2108 34-35 0.1852668943997955 2108 36-37 0.06506702181200552 2108 38-39 0.06047973599444845 2108 40-41 0.08278208995238145 2108 42-43 0.1787611748348681 2108 44-45 0.20061135801216778 2108 46-47 0.16716370261828217 2108 48-49 -0.033453051397117406 2108 50-51 0.1463955091675757 2108 52-53 0.08747343932275697 2108 54-55 0.1082020158138377 2108 56-57 0.0307532653727165 2108 58-59 0.1693947531384481 2108 60-61 0.03164126412785606 2108 62-63 0.21173953498841058 2108 64-65 0.06417410917192257 2108 66-67 0.2031726830900169 2108 68-69 0.048037892215944566 2108 70-71 0.1787350872001241 2108 72-73 0.46045789949688043 2108 74-75 0.2313627630518198 2108 76 -0.03242103982013589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 4.0 13 31.0 14 123.0 15 296.0 16 561.0 17 950.0 18 1197.0 19 1274.0 20 1418.0 21 1342.0 22 1363.0 23 1535.0 24 1711.0 25 1941.0 26 2332.0 27 2938.0 28 3667.0 29 4827.0 30 6437.0 31 8686.0 32 12221.0 33 18027.0 34 28363.0 35 49837.0 36 118896.0 37 1184420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.295847491539455 26.8764808532465 8.453875761655338 24.373795893558707 2 15.15891798531076 23.590791516489983 41.320120077172824 19.930170421026432 3 18.146958397907586 26.259937101123626 33.28951222430174 22.303592276667047 4 13.586171048090002 16.702924508972096 41.87629520942686 27.83460923351105 5 13.60315401973875 34.40619417793479 36.66926109634364 15.321390705982818 6 26.59615868558675 38.87862882099673 19.23125581855861 15.293956674857915 7 23.019146065932432 35.12484203086088 24.19269003395219 17.663321869254496 8 19.924051050675263 41.36226809992863 23.152947130015306 15.560733719380801 9 20.66174458435724 18.582258776483464 26.589833044324866 34.16616359483442 10-11 23.017874061983036 27.08412690336483 31.023557513142897 18.874441521509244 12-13 20.3830725839832 25.48215825379298 32.03394119078822 22.100827971435606 14-15 19.675391467810048 29.3768280759462 26.439392793444433 24.508387662799315 16-17 18.736583796182337 34.158600328108264 28.63765626740411 18.46715960830529 18-19 18.203235978047275 32.4396416936767 31.410315470730843 17.946806857545184 20-21 18.433949991680407 30.358952638823762 32.07072616986547 19.13637119963036 22-23 18.862718458083688 30.62153550816214 31.686580977146555 18.829165056607614 24-25 18.68322838727776 31.13264044642526 31.5177482367275 18.666382929569487 26-27 18.331673998451592 31.47731913822764 31.478041086415136 18.712965776905634 28-29 18.184946623964006 31.469102680284212 31.732166848414256 18.613783847337523 30-31 18.30324299125824 31.13250293248478 31.91086621406245 18.653387862194528 32-33 18.467331500730886 31.191083871127432 31.702979514548286 18.63860511359339 34-35 18.374956511216325 31.026170278011932 32.052333680326846 18.5465395304449 36-37 18.415316852745946 31.262900526540875 32.058556186133366 18.263226434579806 38-39 18.36412728840386 31.079319416005795 31.85699512788109 18.699558167709252 40-41 18.51724906112357 31.039887293574388 31.666744591232938 18.776119054069106 42-43 18.64799044003086 31.035280576568447 31.673860937652552 18.642868045748138 44-45 18.317441305612356 30.735465807846268 32.08684967938625 18.860243207155126 46-47 18.429618302555422 30.768090990224135 32.143436665892004 18.65885404132844 48-49 18.272955545868463 30.935995511544984 32.064709934969656 18.726339007616897 50-51 18.16906376383906 30.91272127711947 32.08079906600532 18.837415893036155 52-53 17.940274945372586 30.72367398745048 32.410695009206556 18.925356057970376 54-55 17.907305978143533 30.643744009548968 32.47137303544147 18.97757697686603 56-57 17.75690010574822 30.66712137942984 32.58781296453928 18.98816555028266 58-59 17.618732974055245 30.82821896069714 32.62336031815225 18.92968774709536 60-61 17.827754163578334 30.471232771222184 32.62239772056893 19.078615344630563 62-63 17.72121523819477 29.987802513479807 32.81581107784802 19.475171170477406 64-65 17.823284960512872 29.740002392742564 32.659492106012245 19.777220540732316 66-67 17.825519562045603 29.913098065316372 32.34736983961749 19.914012533020532 68-69 17.780208718658855 29.84860402723326 32.26919316445705 20.101994089650837 70-71 17.916003734878625 29.732714153897348 32.21270931340665 20.13857279781738 72-73 17.827376000242023 29.26341345353885 32.44111996853681 20.468090577682315 74-75 18.165316508961098 29.472744049428012 31.95947739202062 20.402462049590277 76 18.16346007076467 29.225218956571723 31.838740152282934 20.772580820380664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 5.5 2 5.0 3 4.5 4 4.0 5 7.5 6 11.0 7 23.0 8 35.0 9 60.5 10 142.5 11 328.0 12 457.0 13 658.0 14 1184.0 15 2033.0 16 3302.0 17 4047.0 18 5417.0 19 8405.0 20 12116.5 21 14210.0 22 16998.0 23 23031.5 24 29602.5 25 32928.0 26 36633.5 27 44015.5 28 50668.0 29 53644.0 30 56974.5 31 62872.0 32 66620.5 33 67802.0 34 69663.5 35 73640.0 36 76986.5 37 78218.0 38 78083.5 39 78189.5 40 78154.5 41 76296.5 42 74714.0 43 73234.0 44 69648.0 45 64512.5 46 61483.0 47 57173.0 48 48761.0 49 41312.5 50 37966.0 51 34587.5 52 27776.5 53 21759.5 54 19175.0 55 17051.0 56 13113.5 57 9578.0 58 7856.0 59 7006.0 60 5256.5 61 3673.0 62 2989.0 63 2587.5 64 1801.0 65 1161.5 66 739.5 67 572.0 68 498.0 69 334.5 70 190.5 71 136.0 72 109.5 73 69.0 74 48.5 75 42.0 76 34.5 77 23.0 78 15.0 79 11.0 80 12.0 81 13.0 82 9.5 83 6.0 84 7.5 85 6.5 86 6.5 87 9.0 88 7.5 89 5.5 90 4.5 91 5.5 92 7.0 93 5.5 94 4.0 95 3.0 96 2.0 97 6.5 98 9.5 99 52.5 100 97.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1454398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.92376037203232 #Duplication Level Percentage of deduplicated Percentage of total 1 95.86082965992375 88.11887934723039 2 3.0392036408304106 5.587500544030057 3 0.4408904770473058 1.2158493168722264 4 0.21193798286715046 0.7792854540324732 5 0.1261422807996014 0.5797736396502087 6 0.06416146629326679 0.3538777951596291 7 0.06302501812756318 0.4055447664660778 8 0.03283349320749913 0.24145425294263204 9 0.030805785870700972 0.25486053106054096 >10 0.12580375574609434 2.071624798528484 >50 0.003007254323363567 0.1784271595092172 >100 0.0013591849633174029 0.21292239451808181 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.188127321407207E-4 0.0 0.0 2 6.188127321407207E-4 0.0 0.0 3 6.188127321407207E-4 6.875697023785786E-5 0.0 4 6.188127321407207E-4 2.0627091071357358E-4 0.0 5 6.188127321407207E-4 2.0627091071357358E-4 0.0 6 6.188127321407207E-4 2.0627091071357358E-4 0.0 7 6.188127321407207E-4 2.0627091071357358E-4 0.0 8 6.188127321407207E-4 2.0627091071357358E-4 0.0 9 7.563266726164365E-4 2.0627091071357358E-4 0.0 10 7.563266726164365E-4 2.7502788095143144E-4 0.0 11 7.563266726164365E-4 3.4378485118928933E-4 0.0 12 7.563266726164365E-4 4.1254182142714716E-4 0.0 13 7.563266726164365E-4 4.1254182142714716E-4 0.0 14 7.563266726164365E-4 4.1254182142714716E-4 0.0 15 7.563266726164365E-4 4.1254182142714716E-4 0.0 16 8.250836428542943E-4 4.1254182142714716E-4 0.0 17 8.250836428542943E-4 4.1254182142714716E-4 6.875697023785786E-5 18 8.938406130921523E-4 4.1254182142714716E-4 6.875697023785786E-5 19 8.938406130921523E-4 4.1254182142714716E-4 6.875697023785786E-5 20 9.625975833300101E-4 4.8129879166500505E-4 2.0627091071357358E-4 21 9.625975833300101E-4 4.8129879166500505E-4 2.0627091071357358E-4 22 9.625975833300101E-4 4.8129879166500505E-4 2.7502788095143144E-4 23 0.001031354553567868 5.500557619028629E-4 3.4378485118928933E-4 24 0.001031354553567868 5.500557619028629E-4 5.500557619028629E-4 25 0.001031354553567868 5.500557619028629E-4 6.875697023785787E-4 26 0.001031354553567868 6.188127321407207E-4 8.938406130921523E-4 27 0.001031354553567868 6.188127321407207E-4 0.004125418214271472 28 0.001031354553567868 6.188127321407207E-4 0.011963712821387269 29 0.001031354553567868 6.188127321407207E-4 0.01993952136897878 30 0.001031354553567868 6.188127321407207E-4 0.025921377779672416 31 0.0011688684940435836 6.188127321407207E-4 0.033003345714171774 32 0.0011688684940435836 6.875697023785787E-4 0.04049785547009828 33 0.0011688684940435836 6.875697023785787E-4 0.0502613452438741 34 0.0011688684940435836 6.875697023785787E-4 0.06435652414263496 35 0.0012376254642814414 6.875697023785787E-4 0.08807767887469592 36 0.0013063824345192995 6.875697023785787E-4 0.13036321557097852 37 0.0013751394047571573 6.875697023785787E-4 0.20049532521359353 38 0.001512653345232873 6.875697023785787E-4 0.29620502778469165 39 0.001512653345232873 6.875697023785787E-4 0.42038011603426295 40 0.001512653345232873 6.875697023785787E-4 0.5490931643195329 41 0.001512653345232873 6.875697023785787E-4 0.68495693750954 42 0.001512653345232873 7.563266726164365E-4 0.8324406386697452 43 0.001512653345232873 8.250836428542943E-4 1.0068083151929528 44 0.0015814103154707308 8.250836428542943E-4 1.2034532500732262 45 0.0015814103154707308 8.938406130921523E-4 1.4351642397748072 46 0.0015814103154707308 8.938406130921523E-4 1.7050353479583993 47 0.0017189242559464465 8.938406130921523E-4 2.0019279454454697 48 0.0017189242559464465 8.938406130921523E-4 2.3140845903253444 49 0.0017876812261843045 8.938406130921523E-4 2.6419178244194503 50 0.0017876812261843045 8.938406130921523E-4 2.999660340567025 51 0.0017876812261843045 9.625975833300101E-4 3.347914394821775 52 0.0018564381964221624 9.625975833300101E-4 3.7240150220228574 53 0.0018564381964221624 9.625975833300101E-4 4.103553497735833 54 0.0018564381964221624 9.625975833300101E-4 4.500281216008273 55 0.0019251951666600202 9.625975833300101E-4 4.915573316244934 56 0.0019251951666600202 9.625975833300101E-4 5.350873694820812 57 0.0019251951666600202 9.625975833300101E-4 5.820896343366809 58 0.0019251951666600202 9.625975833300101E-4 6.319178106680565 59 0.0019251951666600202 9.625975833300101E-4 6.819866363952646 60 0.0019251951666600202 9.625975833300101E-4 7.361671289426965 61 0.0019251951666600202 9.625975833300101E-4 7.889999848734665 62 0.001993952136897878 9.625975833300101E-4 8.427060543262574 63 0.001993952136897878 0.001031354553567868 8.96350242505834 64 0.002131466077373594 0.001031354553567868 9.506888760848131 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 175 5.2568794E-9 22.0 8 ACCGCGT 100 3.9343224E-4 21.000002 8 TACACCG 275 7.2759576E-12 19.09091 5 GCCCTGG 110 7.4784266E-4 19.090908 1 ACAGCTC 520 0.0 18.846153 8 TATACAG 490 0.0 18.571428 5 GTGTAGG 305 1.8189894E-12 18.360657 1 GTACTGG 230 6.0827006E-9 18.260868 1 GCTACAC 250 8.6765795E-10 18.199999 3 AACCGTG 235 7.93807E-9 17.872341 7 GCCCCGG 100 0.009691571 17.500002 1 GTACGGG 120 0.0013413 17.500002 1 CGGGGCG 140 1.866107E-4 17.5 69 GTAACAC 280 2.0736479E-10 17.5 3 CTGTACC 225 9.404357E-8 17.11111 9 ACTATAC 350 1.8189894E-12 17.0 3 ACCGCTC 165 3.3966935E-5 16.969696 8 GCTGGCC 145 2.43988E-4 16.896551 8 ACTGTGC 260 2.7659553E-8 16.153847 8 GTCCCAT 455 0.0 16.153847 11 >>END_MODULE