FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve13.3420000000c80f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve13.3420000000c80f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454398
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1755.2568794E-922.08
ACCGCGT1003.9343224E-421.0000028
TACACCG2757.2759576E-1219.090915
GCCCTGG1107.4784266E-419.0909081
ACAGCTC5200.018.8461538
TATACAG4900.018.5714285
GTGTAGG3051.8189894E-1218.3606571
GTACTGG2306.0827006E-918.2608681
GCTACAC2508.6765795E-1018.1999993
AACCGTG2357.93807E-917.8723417
GCCCCGG1000.00969157117.5000021
GTACGGG1200.001341317.5000021
CGGGGCG1401.866107E-417.569
GTAACAC2802.0736479E-1017.53
CTGTACC2259.404357E-817.111119
ACTATAC3501.8189894E-1217.03
ACCGCTC1653.3966935E-516.9696968
GCTGGCC1452.43988E-416.8965518
ACTGTGC2602.7659553E-816.1538478
GTCCCAT4550.016.15384711
Produced by FastQC (version 0.11.2)