##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve12.3420000000c7e4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558060 Sequences flagged as poor quality 0 Sequence length 76 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.43566871622402 34.0 34.0 34.0 34.0 34.0 2 33.57776337239901 34.0 34.0 34.0 34.0 34.0 3 33.616413360204355 34.0 34.0 34.0 34.0 34.0 4 33.64066659820546 34.0 34.0 34.0 34.0 34.0 5 33.644265946112476 34.0 34.0 34.0 34.0 34.0 6 37.41663286394619 38.0 38.0 38.0 37.0 38.0 7 37.43094232571274 38.0 38.0 38.0 37.0 38.0 8 37.33789199388984 38.0 38.0 38.0 37.0 38.0 9 37.37350422961889 38.0 38.0 38.0 37.0 38.0 10-11 37.35482619411319 38.0 38.0 38.0 37.0 38.0 12-13 37.38363317202162 38.0 38.0 38.0 37.0 38.0 14-15 37.35533612312748 38.0 38.0 38.0 37.0 38.0 16-17 37.3543550954392 38.0 38.0 38.0 37.0 38.0 18-19 37.335410703053796 38.0 38.0 38.0 37.0 38.0 20-21 37.340041140905996 38.0 38.0 38.0 37.0 38.0 22-23 37.29433301670025 38.0 38.0 38.0 37.0 38.0 24-25 37.32081402513382 38.0 38.0 38.0 37.0 38.0 26-27 37.309157862983454 38.0 38.0 38.0 37.0 38.0 28-29 37.34517765682965 38.0 38.0 38.0 37.0 38.0 30-31 37.305592531738185 38.0 38.0 38.0 37.0 38.0 32-33 37.29661084938962 38.0 38.0 38.0 37.0 38.0 34-35 37.28906107595343 38.0 38.0 38.0 37.0 38.0 36-37 37.2468739971503 38.0 38.0 38.0 37.0 38.0 38-39 37.23712693991246 38.0 38.0 38.0 37.0 38.0 40-41 37.24186648781176 38.0 38.0 38.0 37.0 38.0 42-43 37.23508433564818 38.0 38.0 38.0 37.0 38.0 44-45 37.25342926459828 38.0 38.0 38.0 37.0 38.0 46-47 37.21656033785605 38.0 38.0 38.0 37.0 38.0 48-49 37.2083854280323 38.0 38.0 38.0 37.0 38.0 50-51 37.20320013349935 38.0 38.0 38.0 37.0 38.0 52-53 37.194741216641205 38.0 38.0 38.0 37.0 38.0 54-55 37.173884510224255 38.0 38.0 38.0 37.0 38.0 56-57 37.16434700845923 38.0 38.0 38.0 37.0 38.0 58-59 37.14372873958641 38.0 38.0 38.0 37.0 38.0 60-61 37.156774129366006 38.0 38.0 38.0 37.0 38.0 62-63 37.095639449058446 38.0 38.0 38.0 37.0 38.0 64-65 37.070324313569444 38.0 38.0 38.0 37.0 38.0 66-67 37.06709722347021 38.0 38.0 38.0 37.0 38.0 68-69 37.02661547052103 38.0 38.0 38.0 36.0 38.0 70-71 37.022812343555444 38.0 38.0 38.0 36.0 38.0 72-73 36.75955804012682 38.0 38.0 38.0 35.5 38.0 74-75 36.90805585150764 38.0 38.0 38.0 36.0 38.0 76 36.04263507182009 38.0 37.0 38.0 32.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.004490955395873186 1101 2 0.03153049381759132 1101 3 0.019982772710974928 1101 4 0.033655430053094904 1101 5 0.009426432686289843 1101 6 0.0555134911596582 1101 7 -0.001593531408353499 1101 8 0.042909539526704066 1101 9 0.06735726263914898 1101 10-11 0.07398376178043264 1101 12-13 0.059115585734105025 1101 14-15 0.046296674045628095 1101 16-17 0.03676250210941845 1101 18-19 0.0023043686947303854 1101 20-21 0.05862749198274031 1101 22-23 0.027144704216745197 1101 24-25 0.013617749674772028 1101 26-27 0.042634590497478086 1101 28-29 0.01729911820618213 1101 30-31 0.02446287726347407 1101 32-33 0.00950290271823917 1101 34-35 0.06746494887585897 1101 36-37 0.08742082762317693 1101 38-39 0.06984699775654235 1101 40-41 -0.004583951924843177 1101 42-43 0.014010491031719141 1101 44-45 0.014676437785603014 1101 46-47 0.08999260105781559 1101 48-49 0.052120725045483596 1101 50-51 0.04593426879792872 1101 52-53 0.07197213366094246 1101 54-55 0.07918464603768172 1101 56-57 0.005664992699124127 1101 58-59 0.008961101144002726 1101 60-61 -0.07458589024976447 1101 62-63 -0.00809625975991679 1101 64-65 0.0016829893041290234 1101 66-67 0.07247889837931609 1101 68-69 0.06605067322683311 1101 70-71 -0.004318718929276599 1101 72-73 0.20316506378054555 1101 74-75 0.01686291821923902 1101 76 0.010181136422680481 1102 1 0.06002584239322317 1102 2 0.04938422090224748 1102 3 0.03887462767953309 1102 4 0.094246978911805 1102 5 0.033913294674796646 1102 6 0.05972919751981465 1102 7 0.05876307540103909 1102 8 0.19302482478055083 1102 9 0.0708286177728823 1102 10-11 0.11089528687427475 1102 12-13 0.0820031826399159 1102 14-15 0.07201583511281484 1102 16-17 0.025576112145479613 1102 18-19 0.009515119017756035 1102 20-21 0.0024356845915107783 1102 22-23 0.053989282434891095 1102 24-25 0.0022770533516123237 1102 26-27 0.04381295313316258 1102 28-29 -0.005615517703859041 1102 30-31 0.05123262397567885 1102 32-33 -0.03716820230895479 1102 34-35 -0.015399066620688018 1102 36-37 -0.03640458953911008 1102 38-39 0.11554299984582173 1102 40-41 0.10942313563521111 1102 42-43 0.1455216389724967 1102 44-45 0.1351639960497053 1102 46-47 0.16186946582892858 1102 48-49 0.1373165786042776 1102 50-51 0.11073940053478992 1102 52-53 0.15252016642757127 1102 54-55 0.16476756789683122 1102 56-57 0.10882615524380412 1102 58-59 0.1355513768195351 1102 60-61 0.05837701155691377 1102 62-63 0.11189216698000592 1102 64-65 0.16678637682985453 1102 66-67 0.09937897026059517 1102 68-69 0.1907197073211151 1102 70-71 0.12278325928391354 1102 72-73 0.4125970725480883 1102 74-75 0.2127936868166671 1102 76 0.432957910218164 1103 1 -0.029138299228975484 1103 2 0.01636536166233782 1103 3 0.025463316001975045 1103 4 0.05651307955118767 1103 5 0.09836843971276465 1103 6 0.10362515663549487 1103 7 0.07507498540251589 1103 8 0.13376468160443267 1103 9 0.13202494801601716 1103 10-11 0.09284833416528926 1103 12-13 0.07474138796273877 1103 14-15 0.0855366945193552 1103 16-17 0.02359458704777495 1103 18-19 0.08871927778095312 1103 20-21 -0.009088690468615823 1103 22-23 0.08620908765166035 1103 24-25 0.04601921988349034 1103 26-27 0.05408702267940413 1103 28-29 -0.01569310327191431 1103 30-31 0.043520210699043105 1103 32-33 -0.002790180253036567 1103 34-35 0.05923601976782322 1103 36-37 -0.011304523672386324 1103 38-39 0.08230861235340825 1103 40-41 0.011240350646559705 1103 42-43 0.08673489848232663 1103 44-45 0.097574913723669 1103 46-47 0.16587819926846237 1103 48-49 0.09295734306331127 1103 50-51 0.12490345010007076 1103 52-53 0.14327011499077003 1103 54-55 0.13779316570293076 1103 56-57 0.07834472979771334 1103 58-59 0.07415315425445357 1103 60-61 0.0029298082690090155 1103 62-63 0.08918276166307493 1103 64-65 0.06756081713532325 1103 66-67 0.07313526983036667 1103 68-69 0.1540915265820928 1103 70-71 0.061808556063901676 1103 72-73 0.3474983921379362 1103 74-75 0.11757703316838075 1103 76 0.37138334112507465 2116 1 -0.0012439319383830139 2116 2 0.012743932699287086 2116 3 0.044793575672287034 2116 4 0.0531490817682041 2116 5 0.07469387050397813 2116 6 0.06595473949792563 2116 7 0.146693030786885 2116 8 0.1353976130763641 2116 9 0.030894116767861135 2116 10-11 0.04827185049783367 2116 12-13 0.038121428257916534 2116 14-15 0.0605375353460218 2116 16-17 0.06594403184877251 2116 18-19 0.08540976095912356 2116 20-21 0.04938101420080443 2116 22-23 0.03185713004591406 2116 24-25 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0.0024364623368597904 1117 7 0.0719610431909885 1117 8 0.14382685238554416 1117 9 0.04282079839228459 1117 10-11 -0.049263288138014616 1117 12-13 0.03599259792477483 1117 14-15 0.0737598839448026 1117 16-17 0.10099307094522203 1117 18-19 -0.04189339411413329 1117 20-21 -0.05593037340223361 1117 22-23 -0.29802174436537143 1117 24-25 -0.18461466227252998 1117 26-27 -0.12259577298244295 1117 28-29 -0.0909542366470859 1117 30-31 -0.19323998804512144 1117 32-33 0.03847929697438701 1117 34-35 0.10028879595531492 1117 36-37 0.016627780532189718 1117 38-39 0.03977899271259133 1117 40-41 0.0882211246084097 1117 42-43 0.0678224732930488 1117 44-45 0.1220829966149708 1117 46-47 0.12913156589443986 1117 48-49 0.11204611806440568 1117 50-51 0.10623778819048368 1117 52-53 0.08444003619666063 1117 54-55 0.0577272472009156 1117 56-57 0.11467451544186957 1117 58-59 0.17773308992240544 1117 60-61 0.1255511551033308 1117 62-63 0.035194542842162946 1117 64-65 0.18645559584734173 1117 66-67 0.12643912722383988 1117 68-69 0.1972244603118085 1117 70-71 0.24961995603309362 1117 72-73 0.4471947232801199 1117 74-75 0.3408522193473402 1117 76 0.49100921281507226 2117 1 0.0793532853243093 2117 2 0.08410829776295259 2117 3 0.10361860496319508 2117 4 0.03680103365901033 2117 5 0.011027865899350786 2117 6 0.09969982811482225 2117 7 0.09777654924953083 2117 8 0.1542586510042696 2117 9 0.14200464459263884 2117 10-11 0.08099500222849088 2117 12-13 0.18787738061483594 2117 14-15 0.1159370425780395 2117 16-17 0.17644713746391716 2117 18-19 0.1749036844295162 2117 20-21 0.18463175072799487 2117 22-23 0.09044702443711827 2117 24-25 0.14437419945921448 2117 26-27 0.08723578554025124 2117 28-29 0.15449027209165678 2117 30-31 0.160443355796005 2117 32-33 0.22579998064353646 2117 34-35 0.20351339514750322 2117 36-37 0.08585561555108256 2117 38-39 0.16580926093499215 2117 40-41 0.1455223184672434 2117 42-43 0.19219063782504264 2117 44-45 0.050382949689726786 2117 46-47 0.1714443183335419 2117 48-49 0.2069070840335243 2117 50-51 0.12270048837847725 2117 52-53 0.21610925064502595 2117 54-55 0.08729806833512299 2117 56-57 0.20661200986410222 2117 58-59 0.2388588351845371 2117 60-61 0.18532081836432468 2117 62-63 0.29885669835184814 2117 64-65 0.2799303958392798 2117 66-67 0.21334410504230306 2117 68-69 0.24095231116438498 2117 70-71 0.1814021653196889 2117 72-73 0.49295288265898307 2117 74-75 0.37344770358420476 2117 76 0.6201083344781253 1116 1 0.11027365347156604 1116 2 0.038366000844185066 1116 3 0.01568543169388903 1116 4 0.0380375282568437 1116 5 -0.0927996651130556 1116 6 0.006338483577010834 1116 7 0.09502544880935204 1116 8 0.161995745842205 1116 9 0.0912483420716299 1116 10-11 0.06903321751460822 1116 12-13 0.11846861710942846 1116 14-15 0.046139123410370075 1116 16-17 0.08156518468280893 1116 18-19 0.11149732188450656 1116 20-21 0.06248689278481834 1116 22-23 0.07011289078365479 1116 24-25 0.09016351518550181 1116 26-27 -0.010379260478153185 1116 28-29 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2118 10-11 0.03044453814042214 2118 12-13 0.150760582676142 2118 14-15 0.001975360344168564 2118 16-17 0.1591138626905959 2118 18-19 0.05638408146638341 2118 20-21 0.022630364807774583 2118 22-23 0.0807602228872284 2118 24-25 0.10130770456857618 2118 26-27 0.07473175953960975 2118 28-29 0.10498949617153386 2118 30-31 0.11536386058328674 2118 32-33 0.12335314825204335 2118 34-35 0.10049609066474119 2118 36-37 0.19412624087438957 2118 38-39 0.06754117321209918 2118 40-41 0.071725970659827 2118 42-43 0.16895012210656546 2118 44-45 0.1782794741531717 2118 46-47 0.15061452300077605 2118 48-49 0.1746166068670476 2118 50-51 0.07676357702856507 2118 52-53 0.16816421897382128 2118 54-55 0.07822224288298685 2118 56-57 0.1924107195918907 2118 58-59 0.21233066294003322 2118 60-61 0.20482959748572682 2118 62-63 0.2120078124560223 2118 64-65 0.24368321403974136 2118 66-67 0.1713214423186784 2118 68-69 0.1696769717603317 2118 70-71 0.13010896511277537 2118 72-73 0.5460472911540819 2118 74-75 0.31538008629338066 2118 76 0.7175814700738243 1119 1 0.0782457916246102 1119 2 0.018700501699989047 1119 3 0.008869084112248515 1119 4 0.051443520027511624 1119 5 -0.05436964278277401 1119 6 0.015282952580989217 1119 7 0.006587605212025949 1119 8 0.007387829359736031 1119 9 -0.1373802157547388 1119 10-11 -0.03758983708690522 1119 12-13 0.059910535700240075 1119 14-15 -0.0939241835120157 1119 16-17 0.03623459446051669 1119 18-19 0.029744082859778587 1119 20-21 0.07964788619908347 1119 22-23 0.07966713523791924 1119 24-25 0.027458802021968154 1119 26-27 -0.028304017622147626 1119 28-29 0.015231540748608552 1119 30-31 0.04345008860100563 1119 32-33 0.04455356158079127 1119 34-35 0.0524504658100966 1119 36-37 0.045247065537417086 1119 38-39 0.07279979340125209 1119 40-41 0.1102899822573562 1119 42-43 0.1421478451783429 1119 44-45 0.16443251143301296 1119 46-47 0.13561997193228592 1119 48-49 0.10707236795237662 1119 50-51 0.09690279486641629 1119 52-53 0.05285371753438284 1119 54-55 0.03684952424241317 1119 56-57 0.05176046677125612 1119 58-59 0.17753072860269725 1119 60-61 0.04332501504418218 1119 62-63 0.1852664681631282 1119 64-65 0.13154015662053098 1119 66-67 0.16822745328757804 1119 68-69 0.20198897053177234 1119 70-71 0.10679396450543521 1119 72-73 0.40013141701011534 1119 74-75 0.29352701357527167 1119 76 0.13354036761035104 2119 1 0.07473314342868775 2119 2 0.028343432575169913 2119 3 0.05414289050679599 2119 4 -0.023011896252292274 2119 5 0.008596182325447899 2119 6 0.05811912000219621 2119 7 -0.011739389827525315 2119 8 0.038498792558520734 2119 9 0.11912241113053312 2119 10-11 0.04510690291293429 2119 12-13 0.06004444649331475 2119 14-15 -0.011116575077558366 2119 16-17 0.09822959452257152 2119 18-19 0.04730131646811486 2119 20-21 0.09124676640251295 2119 22-23 0.07585877207456093 2119 24-25 0.07749763840825352 2119 26-27 0.07456431884930481 2119 28-29 0.04547614511385234 2119 30-31 0.032018004612879736 2119 32-33 0.12748250199330613 2119 34-35 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2104 46-47 -0.13407851471649224 2104 48-49 -0.24415264661784164 2104 50-51 -0.06346777442058027 2104 52-53 -0.003022284117925267 2104 54-55 -0.07251372466630812 2104 56-57 -0.25937965543286623 2104 58-59 -0.0843058553017002 2104 60-61 -0.19105073545650697 2104 62-63 -0.14898337355213442 2104 64-65 -0.20754029434319676 2104 66-67 -0.16902215168568802 2104 68-69 -0.4737301649456427 2104 70-71 -0.25174918880949093 2104 72-73 -0.08737630274134744 2104 74-75 -0.013686768922312353 2104 76 -0.3865140718500939 2111 1 0.027331922598115455 2111 2 0.048047440961049404 2111 3 -0.014149986887197485 2111 4 0.029048343270630994 2111 5 0.03232780596444229 2111 6 -0.01765034797738707 2111 7 -0.017461886550321992 2111 8 0.07868090877428102 2111 9 -0.03590794548050269 2111 10-11 0.05527900578537981 2111 12-13 0.014286281631946451 2111 14-15 0.059038795896888985 2111 16-17 0.05126877319391099 2111 18-19 0.04491306373790138 2111 20-21 0.07813591142245002 2111 22-23 -0.09260511774583335 2111 24-25 -0.004033664428554573 2111 26-27 -0.0025363727045473183 2111 28-29 -0.021638229779149754 2111 30-31 7.562647299934611E-4 2111 32-33 0.051661032634847004 2111 34-35 0.050169959802069286 2111 36-37 0.006521071243511756 2111 38-39 -0.023864916504415135 2111 40-41 -0.011610024821379739 2111 42-43 0.023718360245034376 2111 44-45 -0.04569181866392569 2111 46-47 0.03669092167552179 2111 48-49 -0.11646560002278505 2111 50-51 0.0643325296165429 2111 52-53 -0.0029181227981567304 2111 54-55 0.024763178080156933 2111 56-57 -0.07450326652849526 2111 58-59 0.11110059680754603 2111 60-61 -0.10901574852368867 2111 62-63 -0.04169470281227916 2111 64-65 -0.0384684332618761 2111 66-67 -0.06825252173309337 2111 68-69 -0.28797139092407775 2111 70-71 -0.10400540691257021 2111 72-73 0.11684291368375455 2111 74-75 0.0958648149116641 2111 76 -0.19526868223653082 2110 1 0.020158522847047777 2110 2 -0.025091979959640298 2110 3 -0.02486022015202849 2110 4 -0.0340807823869369 2110 5 0.01392253583166081 2110 6 0.06170180658108393 2110 7 0.006835332402076233 2110 8 -0.22229327612777894 2110 9 -0.1289682242039163 2110 10-11 -0.049520308941062297 2110 12-13 -0.11191342042408792 2110 14-15 -0.051624127637062145 2110 16-17 -0.07634865304336813 2110 18-19 -0.05053054970770887 2110 20-21 -0.18661214044290375 2110 22-23 -0.15431458968940603 2110 24-25 -0.12480540999119683 2110 26-27 -0.06770688370390587 2110 28-29 -0.042915368204923254 2110 30-31 -0.02045882715857772 2110 32-33 -0.10491464008166673 2110 34-35 -0.09752546056051159 2110 36-37 -0.1661958362750724 2110 38-39 -0.25464574534243667 2110 40-41 -0.16007599202438882 2110 42-43 -0.2621771292671724 2110 44-45 -0.09689124636451396 2110 46-47 0.005738201619251981 2110 48-49 -0.12439420079920183 2110 50-51 0.002289399253569968 2110 52-53 0.00932195858953122 2110 54-55 -0.10104021604028901 2110 56-57 -0.1537818576512251 2110 58-59 -0.08468734175564663 2110 60-61 -0.1680621336466075 2110 62-63 -0.246368079348656 2110 64-65 -0.26523290955110923 2110 66-67 -0.2366235788384543 2110 68-69 -0.2447460286151042 2110 70-71 -0.10453629975680911 2110 72-73 0.20798161898899536 2110 74-75 -0.08228047118558379 2110 76 -0.09998491851890634 2109 1 0.09242122359959382 2109 2 0.029467288264690694 2109 3 -0.046250292733688525 2109 4 -0.13023644532657386 2109 5 -0.04141040833292209 2109 6 -0.1849229260231624 2109 7 -0.05124801171492521 2109 8 -0.6109578640658029 2109 9 -0.10621308900543625 2109 10-11 -0.19468476103236299 2109 12-13 -0.37889482563674903 2109 14-15 -0.14094580577553728 2109 16-17 -0.11909475255693991 2109 18-19 -0.27247169769526636 2109 20-21 -0.31198232963228634 2109 22-23 -0.023275387604620335 2109 24-25 -0.08152215256694006 2109 26-27 -5.826943949429619E-4 2109 28-29 -0.10882084538966552 2109 30-31 -0.1336904139734827 2109 32-33 -0.2679538827158936 2109 34-35 -0.05077351630963989 2109 36-37 -0.04031163410999028 2109 38-39 0.026798587760687553 2109 40-41 -0.18667010247507676 2109 42-43 -0.23599513911665326 2109 44-45 -0.19878367762268567 2109 46-47 -0.021074503276778955 2109 48-49 -0.4612101954515637 2109 50-51 -0.2763470685044709 2109 52-53 -0.40060091625811367 2109 54-55 -0.2551977272770358 2109 56-57 -0.4199473886384766 2109 58-59 -0.21292677006231742 2109 60-61 -0.43005516415096423 2109 62-63 -0.34995378550092227 2109 64-65 -0.5335777285329968 2109 66-67 -0.24493633567319506 2109 68-69 -0.6626280435533047 2109 70-71 -0.27326907426108704 2109 72-73 0.10762115036696684 2109 74-75 -0.052754679147895445 2109 76 -1.0039587817193976 2108 1 0.020380964001411428 2108 2 0.024884505813389524 2108 3 -0.053849657629932324 2108 4 -0.03084302780681014 2108 5 0.012159483719329955 2108 6 -0.025629689095389097 2108 7 0.010922345340048878 2108 8 -0.33817893862825343 2108 9 -0.032380704937700955 2108 10-11 -0.09795309429565435 2108 12-13 -0.25483804102117347 2108 14-15 0.01048123669614398 2108 16-17 -0.07337109969009958 2108 18-19 -0.06625418716982523 2108 20-21 -0.13713752036072435 2108 22-23 0.062039297710057895 2108 24-25 0.04960973797680168 2108 26-27 0.07964939631106205 2108 28-29 0.06286089306731668 2108 30-31 0.009661352853989058 2108 32-33 -0.07954776665000196 2108 34-35 0.03494030650103497 2108 36-37 -0.1330012998142891 2108 38-39 -0.15713482066691853 2108 40-41 -0.09092047888216825 2108 42-43 0.012675348995045965 2108 44-45 0.04617342977420691 2108 46-47 -0.00894051165479226 2108 48-49 -0.16603738184502248 2108 50-51 -0.08798532243752533 2108 52-53 -0.14262251350749722 2108 54-55 0.005642973518703798 2108 56-57 -0.12213926017015808 2108 58-59 0.049359885796853575 2108 60-61 -0.12390996741989824 2108 62-63 0.05276240916369801 2108 64-65 -0.06115138898731942 2108 66-67 -0.00970957225447222 2108 68-69 -0.11655161230000743 2108 70-71 -0.11061979070984052 2108 72-73 0.2390986649155451 2108 74-75 0.07024790894163147 2108 76 -0.21373198782237068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 49.0 14 154.0 15 354.0 16 695.0 17 1089.0 18 1373.0 19 1474.0 20 1552.0 21 1452.0 22 1642.0 23 1787.0 24 2087.0 25 2475.0 26 2981.0 27 3939.0 28 4840.0 29 6300.0 30 8232.0 31 10972.0 32 15053.0 33 20933.0 34 31743.0 35 53407.0 36 121180.0 37 1262292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.42617100753501 26.173767377379566 8.711538708393771 24.688522906691652 2 15.160841045916076 23.577076620926025 41.192444450149544 20.069637883008358 3 17.967664916627086 26.71623685865756 33.14538592865486 22.170712296060486 4 13.434591735876666 16.80570709728765 42.08528554741153 27.674415619424153 5 14.334492895010461 33.10411665789508 35.85439585125091 16.70699459584355 6 25.804141047199725 38.727905215460254 19.795194023336713 15.672759714003313 7 22.44457851430625 35.080163793435425 24.729342900786875 17.745914791471446 8 19.61214587371475 40.95323671745633 23.397751049381924 16.036866359447004 9 20.949963416043026 18.306612068854857 25.691886063437863 35.05153845166425 10-11 22.754194318575667 26.705037033233637 31.532771523561355 19.007997124629348 12-13 20.501039754566577 25.353548643826297 31.868605830327457 22.27680577127967 14-15 19.830590606266767 28.78008549093103 26.411595188888747 24.977728713913454 16-17 19.128499544305097 33.40940015147042 28.299263186270107 19.16283711795438 18-19 18.816284353619245 31.852303505641633 30.850127722937497 18.481284417801625 20-21 19.029209401435118 29.96813344800585 31.133236203997278 19.86942094656175 22-23 19.21796978293519 30.20371487619219 31.061608667188683 19.516706673683938 24-25 19.13989191687098 30.81120110907154 30.855711590054298 19.193195384003182 26-27 18.78762692065774 30.99492317369036 30.891653723219903 19.325796182431997 28-29 18.683715646380755 31.065010333363286 31.10384067365827 19.14743334659769 30-31 18.84420368920324 30.736364453230298 31.327227449520556 19.092204408045905 32-33 18.88396467401769 30.60613840288564 31.190551069920286 19.319345853176387 34-35 18.923501020499852 30.46262659974584 31.524652452408763 19.089219927345543 36-37 19.1218245767172 30.775611978999525 31.356943891762835 18.74561955252044 38-39 18.980045697855026 30.515609154974776 31.19555729561121 19.30878785155899 40-41 19.116080253648768 30.433487798929438 31.08304558232674 19.367386365095054 42-43 19.295052822099276 30.399727866706034 31.028298011629847 19.276921299564844 44-45 18.89375248706725 30.113313993042627 31.374080587397145 19.618852932492974 46-47 18.961432807465695 30.134847181751667 31.566948641259003 19.33677136952364 48-49 18.831752307356584 30.29010436055094 31.49140597923058 19.386737352861893 50-51 18.7283224009345 30.203329781908273 31.561428956522857 19.50691886063438 52-53 18.401569901030772 30.170179582301067 31.848067468518543 19.58018304814962 54-55 18.35137927936023 30.046885229066916 31.95149737494063 19.65023811663222 56-57 18.187232840840533 30.027405876538772 32.09953403591646 19.685827246704235 58-59 18.03059574085722 30.137286112216476 32.178638820071114 19.65347932685519 60-61 18.410074066467274 29.756331591851403 32.07379690127466 19.75979744040666 62-63 18.256260991232686 29.29466772781536 32.16814500083437 20.28092628011758 64-65 18.36774578642671 29.048111112537384 32.20479314018716 20.379349960848746 66-67 18.356995237667356 29.25728149108507 31.762448172727627 20.623275098519954 68-69 18.39836078199813 29.13154820738611 31.522855345750482 20.947235664865282 70-71 18.483723348266434 29.067173279591284 31.534921633313225 20.914181738829058 72-73 18.435073103731565 28.643344928950103 31.689183985212377 21.23239798210595 74-75 18.748283121317534 28.787498555896434 31.210415516732347 21.25380280605368 76 18.734002541622274 28.57008074143486 31.31747172766132 21.37844498928154 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 2.0 2 1.5 3 1.5 4 1.0 5 1.5 6 2.5 7 6.5 8 10.0 9 23.5 10 60.5 11 169.0 12 254.0 13 347.5 14 661.0 15 1265.5 16 2231.0 17 2812.0 18 3828.5 19 6267.0 20 9538.0 21 11387.0 22 13739.0 23 19098.0 24 25569.5 25 29034.0 26 32659.5 27 40018.5 28 47167.0 29 50582.0 30 54295.5 31 61466.5 32 67343.0 33 69762.0 34 72097.5 35 77041.5 36 81614.0 37 83578.0 38 84070.0 39 85338.5 40 86397.0 41 86000.5 42 85322.0 43 84517.5 44 81315.5 45 75244.5 46 71571.0 47 67410.0 48 58563.0 49 49723.5 50 45570.0 51 42119.0 52 34451.5 53 26965.5 54 23696.0 55 20932.5 56 15923.5 57 11842.5 58 10007.0 59 8958.0 60 6787.0 61 4808.5 62 3952.0 63 3389.5 64 2335.5 65 1540.5 66 998.5 67 760.0 68 637.5 69 424.0 70 269.0 71 205.0 72 156.5 73 90.5 74 57.5 75 42.0 76 36.0 77 22.5 78 15.0 79 15.0 80 12.0 81 6.5 82 4.5 83 5.0 84 5.0 85 5.0 86 5.5 87 6.0 88 6.5 89 5.5 90 4.5 91 4.0 92 3.0 93 5.0 94 5.0 95 4.0 96 5.0 97 7.5 98 13.0 99 58.0 100 100.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1558060.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.68884384134999 #Duplication Level Percentage of deduplicated Percentage of total 1 95.78984752433911 87.82860371245856 2 3.151767791798847 5.77963889772882 3 0.4458884948129042 1.226490017146649 4 0.19500851456574886 0.7152042095901029 5 0.1152482386745934 0.5283488879412714 6 0.05960633865686587 0.32791417662383987 7 0.03940185588434894 0.25288974278676135 8 0.036293382110307165 0.26621585958291194 9 0.021562350791507215 0.1779324313117423 >10 0.13836029121676302 2.188506416108036 >50 0.005163632948183818 0.3084866284392102 >100 0.00170261460725854 0.32582383260078174 >500 1.489695935998523E-4 0.0739451876813489 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.567295226114527E-4 0.0 0.0 2 3.209119032643159E-4 0.0 0.0 3 3.209119032643159E-4 0.0 0.0 4 3.209119032643159E-4 0.0 0.0 5 3.8509428391717904E-4 6.418238065286317E-5 0.0 6 3.8509428391717904E-4 6.418238065286317E-5 0.0 7 3.8509428391717904E-4 6.418238065286317E-5 0.0 8 3.8509428391717904E-4 6.418238065286317E-5 0.0 9 3.8509428391717904E-4 6.418238065286317E-5 0.0 10 6.418238065286318E-4 6.418238065286317E-5 0.0 11 6.418238065286318E-4 1.2836476130572634E-4 0.0 12 6.418238065286318E-4 1.2836476130572634E-4 6.418238065286317E-5 13 6.418238065286318E-4 1.2836476130572634E-4 6.418238065286317E-5 14 6.418238065286318E-4 1.2836476130572634E-4 6.418238065286317E-5 15 6.418238065286318E-4 1.2836476130572634E-4 6.418238065286317E-5 16 6.418238065286318E-4 1.2836476130572634E-4 6.418238065286317E-5 17 7.060061871814949E-4 1.2836476130572634E-4 6.418238065286317E-5 18 7.701885678343581E-4 1.9254714195858952E-4 6.418238065286317E-5 19 7.701885678343581E-4 1.9254714195858952E-4 6.418238065286317E-5 20 7.701885678343581E-4 3.209119032643159E-4 1.2836476130572634E-4 21 7.701885678343581E-4 3.209119032643159E-4 2.567295226114527E-4 22 7.701885678343581E-4 3.209119032643159E-4 5.776414258757686E-4 23 8.343709484872212E-4 3.209119032643159E-4 5.776414258757686E-4 24 8.343709484872212E-4 3.209119032643159E-4 7.701885678343581E-4 25 8.343709484872212E-4 3.209119032643159E-4 8.985533291400845E-4 26 8.343709484872212E-4 3.209119032643159E-4 0.0012194652324044003 27 9.627357097929477E-4 3.209119032643159E-4 0.003658395697213201 28 9.627357097929477E-4 3.209119032643159E-4 0.010461728046416697 29 9.627357097929477E-4 3.209119032643159E-4 0.016623236589091563 30 0.0010269180904458107 3.8509428391717904E-4 0.022207103705890657 31 0.0010269180904458107 3.8509428391717904E-4 0.02766260606138403 32 0.0010269180904458107 3.8509428391717904E-4 0.03420920888797607 33 0.0011552828517515372 4.4927666457004225E-4 0.04223200646958397 34 0.0011552828517515372 4.4927666457004225E-4 0.057314865923006816 35 0.0012194652324044003 4.4927666457004225E-4 0.077403951067353 36 0.0012194652324044003 4.4927666457004225E-4 0.11989268705954842 37 0.0012836476130572635 4.4927666457004225E-4 0.19813100907538864 38 0.0012836476130572635 4.4927666457004225E-4 0.3107710871211635 39 0.0012836476130572635 5.134590452229054E-4 0.4439495269758546 40 0.0013478299937101266 5.776414258757686E-4 0.6131984647574548 41 0.0013478299937101266 6.418238065286318E-4 0.7873252634686726 42 0.0013478299937101266 6.418238065286318E-4 0.985071178260144 43 0.0013478299937101266 6.418238065286318E-4 1.1930862739560735 44 0.0013478299937101266 6.418238065286318E-4 1.4176604238604418 45 0.0013478299937101266 6.418238065286318E-4 1.698073244932801 46 0.0014120123743629899 6.418238065286318E-4 2.031115618140508 47 0.0015403771356687162 6.418238065286318E-4 2.3832843407827684 48 0.0015403771356687162 6.418238065286318E-4 2.754001771433706 49 0.0016045595163215794 6.418238065286318E-4 3.141470803435041 50 0.0016687418969744425 6.418238065286318E-4 3.5675776285893996 51 0.0016687418969744425 7.701885678343581E-4 3.9800777890453514 52 0.0016687418969744425 8.343709484872212E-4 4.403809866115554 53 0.0017329242776273057 8.343709484872212E-4 4.823562635585279 54 0.001797106658280169 8.343709484872212E-4 5.273545306342503 55 0.001797106658280169 8.343709484872212E-4 5.742461779392321 56 0.001797106658280169 8.343709484872212E-4 6.240581235639192 57 0.001861289038933032 8.343709484872212E-4 6.755003016571891 58 0.001861289038933032 8.343709484872212E-4 7.302735453063425 59 0.001861289038933032 8.985533291400845E-4 7.863946189492061 60 0.001861289038933032 9.627357097929477E-4 8.467709844293545 61 0.001861289038933032 9.627357097929477E-4 9.047148376827593 62 0.0019896538002387586 9.627357097929477E-4 9.603224522804 63 0.0019896538002387586 9.627357097929477E-4 10.169377302542905 64 0.0019896538002387586 0.0010269180904458107 10.751704042206333 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTTA 175 1.2662167E-7 20.0 1 ATAGCCC 190 3.0087358E-7 18.421053 3 GCCCTAC 155 1.9853394E-5 18.064516 1 CCTTAGG 120 0.0013413582 17.500002 2 GTATAGG 320 3.6379788E-12 17.5 1 GTGTAGC 305 7.021299E-10 16.065575 1 GTACAGG 245 2.4795736E-7 15.714286 1 GTGCTAC 290 6.2264007E-9 15.689655 1 GGGGTGC 135 0.0029472343 15.555555 8 TAGGGTG 135 0.0029472343 15.555555 5 GCACTGC 315 1.1077645E-9 15.555555 6 CTAGGGG 140 0.0037547187 15.000001 3 CTGTGCG 235 2.7725546E-6 14.893618 9 GCGTTAT 165 6.521973E-4 14.848485 1 GAGTGTA 1105 0.0 14.570136 70 ACGAGTG 1640 0.0 14.512196 70 GAGCGTC 170 8.1774057E-4 14.411765 7 GTATAGA 415 1.8189894E-11 14.337348 1 ACTGTGC 440 3.6379788E-12 14.318183 8 GTATACG 440 3.6379788E-12 14.318183 1 >>END_MODULE