FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve12.3420000000c7e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve12.3420000000c7e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558060
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTA1751.2662167E-720.01
ATAGCCC1903.0087358E-718.4210533
GCCCTAC1551.9853394E-518.0645161
CCTTAGG1200.001341358217.5000022
GTATAGG3203.6379788E-1217.51
GTGTAGC3057.021299E-1016.0655751
GTACAGG2452.4795736E-715.7142861
GTGCTAC2906.2264007E-915.6896551
GGGGTGC1350.002947234315.5555558
TAGGGTG1350.002947234315.5555555
GCACTGC3151.1077645E-915.5555556
CTAGGGG1400.003754718715.0000013
CTGTGCG2352.7725546E-614.8936189
GCGTTAT1656.521973E-414.8484851
GAGTGTA11050.014.57013670
ACGAGTG16400.014.51219670
GAGCGTC1708.1774057E-414.4117657
GTATAGA4151.8189894E-1114.3373481
ACTGTGC4403.6379788E-1214.3181838
GTATACG4403.6379788E-1214.3181831
Produced by FastQC (version 0.11.2)