FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve11.3420000000c7ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve11.3420000000c7ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493717
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC7100.022.183152
ACCGGCA7400.021.75675853
ATACCGG7500.021.46666551
CCGGCAG7550.020.86092854
GGGGTCT6500.020.46153867
TGGGGTC7100.020.21126766
CGGCAGT8400.019.16666655
TGAGTCC8900.018.87640437
GTCCTGA8750.018.840
GAGTCCT9050.018.56353638
GGCCCTA1702.1206433E-618.52941338
GGCAGTA8600.018.31395356
GTTTCCT10650.018.0751171
AATACCG9250.017.78378550
TCCTGAC9100.017.69230741
ATGAGTC10500.017.036
CTGCGCG1452.439936E-416.8965519
GCAGTAT9350.016.8449257
GAATACC10400.016.82692349
CTGACAG9800.016.78571543