##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve10.3420000000c7a1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456613 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.267267970284486 34.0 34.0 34.0 34.0 34.0 2 33.37327279105706 34.0 34.0 34.0 34.0 34.0 3 33.430810379970524 34.0 34.0 34.0 34.0 34.0 4 33.431998066747994 34.0 34.0 34.0 34.0 34.0 5 33.46344842453006 34.0 34.0 34.0 34.0 34.0 6 37.176104428561324 38.0 38.0 38.0 37.0 38.0 7 37.1887941409283 38.0 38.0 38.0 37.0 38.0 8 37.063242604590236 38.0 38.0 38.0 37.0 38.0 9 37.10036227879334 38.0 38.0 38.0 37.0 38.0 10-11 37.07839625212736 38.0 38.0 38.0 36.5 38.0 12-13 37.11630062343258 38.0 38.0 38.0 37.0 38.0 14-15 37.08591540786743 38.0 38.0 38.0 36.5 38.0 16-17 37.08965284533366 38.0 38.0 38.0 36.5 38.0 18-19 37.064135429245795 38.0 38.0 38.0 36.5 38.0 20-21 37.074875756292165 38.0 38.0 38.0 37.0 38.0 22-23 37.01533660622279 38.0 38.0 38.0 36.0 38.0 24-25 37.05562939504179 38.0 38.0 38.0 36.0 38.0 26-27 37.04135861756005 38.0 38.0 38.0 36.0 38.0 28-29 37.09063697770101 38.0 38.0 38.0 37.0 38.0 30-31 37.03969379649914 38.0 38.0 38.0 36.0 38.0 32-33 37.02874579589775 38.0 38.0 38.0 36.0 38.0 34-35 37.01614533167012 38.0 38.0 38.0 36.0 38.0 36-37 36.964435302994005 38.0 38.0 38.0 36.0 38.0 38-39 36.95284814841004 38.0 38.0 38.0 36.0 38.0 40-41 36.96196724867896 38.0 38.0 38.0 36.0 38.0 42-43 36.9587251383861 38.0 38.0 38.0 36.0 38.0 44-45 36.99459156275552 38.0 38.0 38.0 36.0 38.0 46-47 36.947494976359536 38.0 38.0 38.0 36.0 38.0 48-49 36.93917499019987 38.0 38.0 38.0 36.0 38.0 50-51 36.94189980454657 38.0 38.0 38.0 36.0 38.0 52-53 36.92769047097616 38.0 38.0 38.0 36.0 38.0 54-55 36.917745138894134 38.0 38.0 38.0 36.0 38.0 56-57 36.913650708870506 38.0 38.0 38.0 36.0 38.0 58-59 36.88795067735906 38.0 38.0 38.0 36.0 38.0 60-61 36.896388745672326 38.0 38.0 38.0 36.0 38.0 62-63 36.8199185370445 38.0 38.0 38.0 36.0 38.0 64-65 36.797375486831434 38.0 38.0 38.0 36.0 38.0 66-67 36.794734428430885 38.0 38.0 38.0 36.0 38.0 68-69 36.74747822517031 38.0 38.0 38.0 35.0 38.0 70-71 36.73801277346831 38.0 38.0 38.0 35.0 38.0 72-73 36.475010177720506 38.0 38.0 38.0 34.0 38.0 74-75 36.61558286243498 38.0 38.0 38.0 34.0 38.0 76 35.42364375438088 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03084604643616018 1101 2 0.03132911544244621 1101 3 -0.026168011093815835 1101 4 -0.04153515284480136 1101 5 0.03643987280180738 1101 6 0.0014462581269469865 1101 7 -0.07695858407974043 1101 8 0.14054511674167003 1101 9 -0.03346831870249645 1101 10-11 0.008733068648972164 1101 12-13 0.007869561915818224 1101 14-15 -0.06096312642072377 1101 16-17 0.03820976659861941 1101 18-19 -0.029911361487741317 1101 20-21 -0.04260394005849122 1101 22-23 -0.0075487573644537065 1101 24-25 -0.06081389622814726 1101 26-27 0.06344801502827835 1101 28-29 -0.050439408237544114 1101 30-31 0.007197423258780589 1101 32-33 -0.04185868513150126 1101 34-35 -0.040088721329254895 1101 36-37 -0.05022989166727854 1101 38-39 0.026355841800921098 1101 40-41 -0.11548513978958397 1101 42-43 -0.08521271535960295 1101 44-45 -0.03761589231562823 1101 46-47 -0.04872143935999418 1101 48-49 0.00648784333954211 1101 50-51 -0.039245451464474 1101 52-53 -0.046450665719518724 1101 54-55 0.06372686610425404 1101 56-57 -0.09981459152388084 1101 58-59 -0.042222622141153465 1101 60-61 -0.1656210075583715 1101 62-63 0.008321972858446713 1101 64-65 -0.02791350691019545 1101 66-67 -0.02938839984548025 1101 68-69 0.03499423127141199 1101 70-71 -0.02849536954002474 1101 72-73 0.15224000664519366 1101 74-75 0.028791966425551152 1101 76 -0.017980257745563222 1102 1 0.07994616182909198 1102 2 0.11500128395289266 1102 3 0.06949330289634048 1102 4 0.09518718269828952 1102 5 0.08845532202075646 1102 6 0.16683641725025922 1102 7 0.11778405035291684 1102 8 0.1705298184244839 1102 9 0.18398932473223084 1102 10-11 0.22924413130963472 1102 12-13 0.13669830401949667 1102 14-15 0.1022081913159596 1102 16-17 0.06733940162447283 1102 18-19 0.049241364110407915 1102 20-21 0.02316275800268386 1102 22-23 0.09099569101152838 1102 24-25 0.02282308355102458 1102 26-27 0.1338993674203337 1102 28-29 0.019148291707303144 1102 30-31 0.08123822137303449 1102 32-33 -0.04592880502035257 1102 34-35 0.05923365167910788 1102 36-37 0.057796586389521565 1102 38-39 0.21976156462039143 1102 40-41 0.23823758081319113 1102 42-43 0.2220911253505733 1102 44-45 0.23955819889147278 1102 46-47 0.2839356545841625 1102 48-49 0.2506513271930544 1102 50-51 0.22367629295997205 1102 52-53 0.300624766952275 1102 54-55 0.3092713613118221 1102 56-57 0.20879337869123304 1102 58-59 0.30729338645504356 1102 60-61 0.2613359672254205 1102 62-63 0.2628708155979851 1102 64-65 0.2719253143908986 1102 66-67 0.2675140811877341 1102 68-69 0.29453603626470226 1102 70-71 0.1543338955163307 1102 72-73 0.5612655584773449 1102 74-75 0.3442420309490686 1102 76 0.6539141578483125 1103 1 -0.025816204310629587 1103 2 0.02251445750379588 1103 3 0.11007123932333229 1103 4 0.08593340061431576 1103 5 0.11710057104336613 1103 6 0.18953572032692279 1103 7 0.10894502264596184 1103 8 0.24192876454041112 1103 9 0.1628572492605329 1103 10-11 0.20010933613710336 1103 12-13 0.1780768523791707 1103 14-15 0.20629284200438036 1103 16-17 0.1301661906776843 1103 18-19 0.21707410250918002 1103 20-21 0.0505450459510044 1103 22-23 0.1669824324699718 1103 24-25 0.1183474758678642 1103 26-27 0.16314125081316178 1103 28-29 0.05292697605202079 1103 30-31 0.1325861730983391 1103 32-33 0.027453822890578294 1103 34-35 0.15253128893900936 1103 36-37 0.11176904941859078 1103 38-39 0.1435946429817747 1103 40-41 0.06873222640217591 1103 42-43 0.1532164015701909 1103 44-45 0.1768775937580358 1103 46-47 0.192405822865652 1103 48-49 0.18095389391071848 1103 50-51 0.15847146549099023 1103 52-53 0.22407028794496142 1103 54-55 0.13270838323941092 1103 56-57 0.15613827075310382 1103 58-59 0.14514822242308156 1103 60-61 0.0858115749085826 1103 62-63 0.13118120145554002 1103 64-65 0.1368326745812709 1103 66-67 0.08631401319521359 1103 68-69 0.1936436344289021 1103 70-71 0.13459906634753338 1103 72-73 0.450026394785354 1103 74-75 0.1710854781698714 1103 76 0.564704570902876 2116 1 -0.022965688969975417 2116 2 0.038053592305438144 2116 3 0.021435517009443572 2116 4 0.030026951084217046 2116 5 0.11693604465457952 2116 6 0.04548999729561842 2116 7 0.11017897056188275 2116 8 0.12609644754014937 2116 9 0.07080569128171987 2116 10-11 0.0449953872646347 2116 12-13 0.04489847994691587 2116 14-15 0.05157777396984642 2116 16-17 0.04995383685029253 2116 18-19 0.08522485459565843 2116 20-21 0.06720908210854759 2116 22-23 0.0639066498935037 2116 24-25 -0.08405776601940573 2116 26-27 0.08591380742889498 2116 28-29 -0.11658972768091047 2116 30-31 -0.014450199668715413 2116 32-33 0.16345497074910043 2116 34-35 0.10567283424354912 2116 36-37 0.04945276656712139 2116 38-39 0.08109996378431816 2116 40-41 0.0367978456479392 2116 42-43 0.031662009815633496 2116 44-45 -6.172050080124336E-4 2116 46-47 -0.08249481563066041 2116 48-49 -0.05742475592069951 2116 50-51 -0.09748663767279453 2116 52-53 -0.11289601762454282 2116 54-55 -0.16648381132261392 2116 56-57 -0.05557992997864858 2116 58-59 -0.2650940235534236 2116 60-61 -0.08094022459879824 2116 62-63 -0.1316635745924728 2116 64-65 -0.0712979899809838 2116 66-67 -0.11819850563684042 2116 68-69 -0.03857053684115641 2116 70-71 -0.1491659096191711 2116 72-73 0.2104726247875064 2116 74-75 -0.12730704161872808 2116 76 0.1786290513773281 1117 1 0.032196133687840245 1117 2 0.11408760771426074 1117 3 0.1065698059056217 1117 4 0.12218558715016314 1117 5 -0.04143033014975117 1117 6 0.023846310521427938 1117 7 0.07385597028466151 1117 8 0.16581569313230204 1117 9 0.09603715703423177 1117 10-11 -0.14042971123770798 1117 12-13 0.161483368374725 1117 14-15 0.1649076428535068 1117 16-17 0.16425566570328698 1117 18-19 0.020889099018639 1117 20-21 -0.005521073309978419 1117 22-23 -0.40730457084010396 1117 24-25 -0.17353923743434763 1117 26-27 -0.03723902042018068 1117 28-29 -0.06743955463388573 1117 30-31 -0.11524600226143633 1117 32-33 0.2981816444914216 1117 34-35 0.24259997584607618 1117 36-37 0.11155363309425326 1117 38-39 0.164283601628739 1117 40-41 0.24632294488399964 1117 42-43 0.21204630214117515 1117 44-45 0.2410236298158992 1117 46-47 0.19650880098684098 1117 48-49 0.2523708957802526 1117 50-51 0.1561347809204534 1117 52-53 0.18878491038859835 1117 54-55 0.2790996374126209 1117 56-57 0.28101907783613456 1117 58-59 0.2487407107127524 1117 60-61 0.2777264025106163 1117 62-63 0.1530551398478721 1117 64-65 0.32909585988711854 1117 66-67 0.25363842150476756 1117 68-69 0.36728932776458123 1117 70-71 0.37818572915165305 1117 72-73 0.5544556317575626 1117 74-75 0.48069771928798133 1117 76 0.6271247752064539 2117 1 0.1568781833831565 2117 2 0.07206939881694296 2117 3 0.051273819141464116 2117 4 0.043066757983218906 2117 5 0.042430058685077654 2117 6 0.1255610377967784 2117 7 0.11857046830554197 2117 8 0.21825212311109254 2117 9 0.16695771795420455 2117 10-11 0.17029000352483337 2117 12-13 0.22682900556588947 2117 14-15 0.20703554119918977 2117 16-17 0.23893997587978077 2117 18-19 0.18393385494278647 2117 20-21 0.22283363305992765 2117 22-23 0.10785973087160272 2117 24-25 0.2732142787361127 2117 26-27 0.13644814501657976 2117 28-29 0.2059446421979132 2117 30-31 0.22916430873909377 2117 32-33 0.2930693338467947 2117 34-35 0.29051149965086154 2117 36-37 0.1644069535166537 2117 38-39 0.1931311570225489 2117 40-41 0.23782728709199574 2117 42-43 0.25634503194314107 2117 44-45 0.16077696482722104 2117 46-47 0.2575085749387185 2117 48-49 0.38630261001053867 2117 50-51 0.1469832629512311 2117 52-53 0.2736957336045407 2117 54-55 0.192915089164174 2117 56-57 0.14856786655489174 2117 58-59 0.2457402986959707 2117 60-61 0.26414030142382217 2117 62-63 0.3675197825555685 2117 64-65 0.3942953192954519 2117 66-67 0.3279692629648707 2117 68-69 0.37934006514636565 2117 70-71 0.2778462722122157 2117 72-73 0.5986719902666664 2117 74-75 0.4822283696492562 2117 76 0.7042935626106015 1116 1 0.11390380771749165 1116 2 0.12649153406534452 1116 3 0.10399404613990271 1116 4 0.08652265135453518 1116 5 -0.004143035038794096 1116 6 0.10627635358532217 1116 7 0.1543543686247375 1116 8 0.3286048182342043 1116 9 0.21668004446264888 1116 10-11 0.2051490559900273 1116 12-13 0.27099741108229836 1116 14-15 0.16451258435434823 1116 16-17 0.2726686766488555 1116 18-19 0.2618822969065633 1116 20-21 0.1945476743185992 1116 22-23 0.24209882365125424 1116 24-25 0.3272204757549062 1116 26-27 0.16346531008189658 1116 28-29 0.20069361137556285 1116 30-31 0.21391438970638177 1116 32-33 0.3153662912491342 1116 34-35 0.1708140675626808 1116 36-37 0.150621986523916 1116 38-39 0.259372421820224 1116 40-41 0.21564524684521302 1116 42-43 0.3167739893905974 1116 44-45 0.1344728919711713 1116 46-47 0.2801398104270447 1116 48-49 0.36818820524499074 1116 50-51 0.17969076076391133 1116 52-53 0.299689601037322 1116 54-55 0.2262416443403623 1116 56-57 0.32238312578718364 1116 58-59 0.36379292662831375 1116 60-61 0.37059622218705357 1116 62-63 0.3866724054929236 1116 64-65 0.4306545788113212 1116 66-67 0.39268350885729575 1116 68-69 0.4216572281169988 1116 70-71 0.3224229784467809 1116 72-73 0.6297306557001576 1116 74-75 0.4248127166178577 1116 76 0.8219649272598843 2118 1 0.07996930064508234 2118 2 0.02556550109618172 2118 3 0.0595605211567829 2118 4 0.0885415113287209 2118 5 -0.06261050651222178 2118 6 0.11277492571964132 2118 7 0.133716523659686 2118 8 0.24221552312411632 2118 9 0.05454811823877037 2118 10-11 0.004599347097531847 2118 12-13 0.10880894290381349 2118 14-15 -0.053711862040628944 2118 16-17 0.06163596954052508 2118 18-19 0.052769525002986484 2118 20-21 -0.0763528715682682 2118 22-23 -0.003408802953366319 2118 24-25 0.06150040907512988 2118 26-27 0.07587989877654877 2118 28-29 0.0799506797348144 2118 30-31 0.045318705803218506 2118 32-33 0.0670126775496982 2118 34-35 0.13640761018765346 2118 36-37 0.1739493742799354 2118 38-39 0.126753409204845 2118 40-41 0.048357596257716295 2118 42-43 0.15903502300275818 2118 44-45 0.15535188380005138 2118 46-47 0.11588615760549459 2118 48-49 0.1604314626720864 2118 50-51 0.11374587595654617 2118 52-53 0.18789439429401256 2118 54-55 0.04444506961602457 2118 56-57 0.16331203347166934 2118 58-59 0.2020384723489954 2118 60-61 0.18214740608981117 2118 62-63 0.19829842155310473 2118 64-65 0.25587794160638566 2118 66-67 0.13093188009896295 2118 68-69 0.16370144714205992 2118 70-71 0.08053359544911132 2118 72-73 0.524034758577379 2118 74-75 0.3019481529839467 2118 76 0.6704341918038921 1119 1 0.16199899140323026 1119 2 0.08822359555435355 1119 3 0.03265340851093157 1119 4 0.05125608491567846 1119 5 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1119 66-67 0.20591745360311364 1119 68-69 0.2814976744764621 1119 70-71 0.20010766657776458 1119 72-73 0.46180409150585433 1119 74-75 0.38515584325923413 1119 76 0.0738730653059747 2119 1 0.14886222083277545 2119 2 0.1403056738399755 2119 3 0.13873892095205065 2119 4 0.02837179194660422 2119 5 0.1030625461029544 2119 6 0.1882807496052763 2119 7 0.08314519013041632 2119 8 0.19760232383634957 2119 9 0.15403066165406898 2119 10-11 0.166250453569468 2119 12-13 0.28573679924292605 2119 14-15 0.13845061047047835 2119 16-17 0.2677817840141259 2119 18-19 0.23511114076822537 2119 20-21 0.30398238932711763 2119 22-23 0.3334804834600291 2119 24-25 0.2663171904058643 2119 26-27 0.26944938135047636 2119 28-29 0.19245412314453603 2119 30-31 0.18352828098283425 2119 32-33 0.3066387109540898 2119 34-35 0.22552641680694308 2119 36-37 0.34646350027855277 2119 38-39 0.25819337946399656 2119 40-41 0.27384065922849743 2119 42-43 0.29242781062345813 2119 44-45 0.23635028483721499 2119 46-47 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-0.2739137347616136 2104 62-63 -0.28260661767889417 2104 64-65 -0.31873078887915796 2104 66-67 -0.26175578500178887 2104 68-69 -0.4963030629271685 2104 70-71 -0.28040290221602504 2104 72-73 -0.12907018597117315 2104 74-75 -0.03641908990709197 2104 76 -0.45363052909005575 2111 1 -0.07393192490049216 2111 2 0.0032347290464400658 2111 3 -0.08030890026780924 2111 4 -0.002023964778743448 2111 5 -0.04890405965663547 2111 6 -0.08640588326237264 2111 7 -0.118991300202957 2111 8 0.12248438152590069 2111 9 -0.05294536631429736 2111 10-11 0.026691464047623015 2111 12-13 -0.10696930383826242 2111 14-15 -0.053639305842949625 2111 16-17 -0.11081888855017752 2111 18-19 -0.04756457221262167 2111 20-21 0.05761711666467306 2111 22-23 -0.20448005785481627 2111 24-25 -0.05655483378590276 2111 26-27 -0.15265399316977124 2111 28-29 -0.10347766403000236 2111 30-31 -0.10100844966044775 2111 32-33 -0.11899511375729332 2111 34-35 -0.05900582200009552 2111 36-37 -0.18553284742603182 2111 38-39 -0.06490450090834798 2111 40-41 -0.13385017626767848 2111 42-43 -0.15763947375609177 2111 44-45 -0.2552166419921633 2111 46-47 -0.04060560645250888 2111 48-49 -0.2813550562070688 2111 50-51 -0.0053785829269656915 2111 52-53 -0.09721712707230523 2111 54-55 -0.055910146932184546 2111 56-57 -0.2693452554143647 2111 58-59 -0.13094084651135063 2111 60-61 -0.3318693222811717 2111 62-63 -0.35176502278158495 2111 64-65 -0.3302129589232834 2111 66-67 -0.16896672718066696 2111 68-69 -0.5585341683676148 2111 70-71 -0.31490435641660497 2111 72-73 -0.20962781054028312 2111 74-75 -0.0724860581033866 2111 76 -0.5382523756497335 2110 1 0.08238227463204595 2110 2 -0.08510552532835902 2110 3 -0.13640243943764574 2110 4 -0.18952373489472762 2110 5 -0.09881067723233627 2110 6 -0.05321006536899375 2110 7 -0.17656218016502123 2110 8 -0.37968855293438253 2110 9 -0.20444643113706462 2110 10-11 -0.19613094015156918 2110 12-13 -0.24133573358528082 2110 14-15 -0.14162136555281535 2110 16-17 -0.055251465477859085 2110 18-19 -0.17479936315680789 2110 20-21 -0.2582566788491789 2110 22-23 -0.22988867846300565 2110 24-25 -0.21797429756340136 2110 26-27 -0.1642168055467934 2110 28-29 -0.21548663530681011 2110 30-31 -0.22882933874713984 2110 32-33 -0.24415507766620692 2110 34-35 -0.20797526256284016 2110 36-37 -0.2531901185089467 2110 38-39 -0.39076254830604995 2110 40-41 -0.2547945624434078 2110 42-43 -0.39201464616910187 2110 44-45 -0.36366806969216015 2110 46-47 -0.17058719309643067 2110 48-49 -0.23684915688806285 2110 50-51 -0.12150495377840542 2110 52-53 -0.11660420111137171 2110 54-55 -0.2316699171443659 2110 56-57 -0.37245276389320026 2110 58-59 -0.2989240294698874 2110 60-61 -0.348160742783314 2110 62-63 -0.4208397940788089 2110 64-65 -0.46193501514173363 2110 66-67 -0.41647492590571034 2110 68-69 -0.357084328777141 2110 70-71 -0.451182855315011 2110 72-73 9.488489181634918E-4 2110 74-75 -0.2789687307901403 2110 76 -0.39320121973630506 2109 1 -0.003210545981907842 2109 2 -0.12886222493735744 2109 3 -0.04779490289288191 2109 4 -0.1502620800977894 2109 5 -0.05708396996005405 2109 6 -0.24852770539983027 2109 7 -0.01808568638646335 2109 8 -1.0670553175688653 2109 9 -0.22335093298599418 2109 10-11 -0.3991490581431094 2109 12-13 -0.5827728188149806 2109 14-15 -0.2972642022256409 2109 16-17 -0.42584522021694937 2109 18-19 -0.47577585549380785 2109 20-21 -0.632469519840896 2109 22-23 -0.20081057349155884 2109 24-25 -0.3058898174761211 2109 26-27 -0.11288316891108963 2109 28-29 -0.2740846806787687 2109 30-31 -0.27341745298488007 2109 32-33 -0.4287655244144446 2109 34-35 -0.17947442675148295 2109 36-37 -0.18302629198635856 2109 38-39 -0.012567854713516624 2109 40-41 -0.34253731245017605 2109 42-43 -0.2969359144112076 2109 44-45 -0.3769374759979627 2109 46-47 -0.17436542659658727 2109 48-49 -0.803605444790044 2109 50-51 -0.3818436145081918 2109 52-53 -0.5278898456260066 2109 54-55 -0.38768741296242837 2109 56-57 -0.6588629584276475 2109 58-59 -0.3757217302534528 2109 60-61 -0.6213702209244971 2109 62-63 -0.5979989349835222 2109 64-65 -0.7510967704436169 2109 66-67 -0.30715186679577045 2109 68-69 -0.9496445265118325 2109 70-71 -0.3506051825205958 2109 72-73 0.0825089301755213 2109 74-75 -0.25026016745731994 2109 76 -1.3651702575239781 2108 1 -0.01241282343757888 2108 2 -0.06620882860315191 2108 3 -0.1417766126855753 2108 4 -0.09357985254035128 2108 5 -0.027552611820055972 2108 6 -0.06964184774922444 2108 7 -0.02888883029945788 2108 8 -0.5634681686167582 2108 9 -0.17459001925097084 2108 10-11 -0.1346695110377567 2108 12-13 -0.3255461929828769 2108 14-15 -0.0998612451755676 2108 16-17 -0.1399183224449203 2108 18-19 -0.25931294844413344 2108 20-21 -0.2995449679227278 2108 22-23 0.046237951063723415 2108 24-25 -0.03527280171118008 2108 26-27 0.02556638576434267 2108 28-29 -0.05159474582234225 2108 30-31 -0.009910123468735321 2108 32-33 -0.21364886882191314 2108 34-35 -0.043963688144096125 2108 36-37 -0.17840772313753206 2108 38-39 -0.21588525751730003 2108 40-41 -0.1188419945713548 2108 42-43 0.005927801876907779 2108 44-45 -0.05790003489599371 2108 46-47 -0.09170574490064354 2108 48-49 -0.4551118505097662 2108 50-51 -0.10470048346343219 2108 52-53 -0.23681366187286557 2108 54-55 -0.04917538031881463 2108 56-57 -0.32727943644330537 2108 58-59 0.003909648231704921 2108 60-61 -0.34846813132346455 2108 62-63 -0.07810928868575928 2108 64-65 -0.13511419094798782 2108 66-67 -0.03404102895689931 2108 68-69 -0.3660365560781287 2108 70-71 -0.11205583288949583 2108 72-73 0.18471277363740057 2108 74-75 -0.0421202934410303 2108 76 -0.5047168580042651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 63.0 13 458.0 14 954.0 15 936.0 16 1059.0 17 1381.0 18 1597.0 19 1690.0 20 1792.0 21 1914.0 22 2015.0 23 2362.0 24 2769.0 25 3527.0 26 4242.0 27 5353.0 28 6748.0 29 8688.0 30 11106.0 31 14411.0 32 19259.0 33 26616.0 34 37946.0 35 61034.0 36 128311.0 37 1110380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.372582403550155 25.458804403643526 8.615952635293407 24.552660557512915 2 16.944594717055743 22.890035232443505 38.226926594041515 21.938443456459236 3 19.500443837862218 24.67072585511732 31.678215147056903 24.15061515996356 4 14.528498647204167 16.4172638854658 39.22263497579659 29.83160249153344 5 13.630868322608682 35.42505799412747 36.63073170430307 14.31334197896078 6 28.726229959501943 37.313479970314695 18.458231527523097 15.502058542660269 7 24.768143631836324 33.44024802744449 22.7733790649953 19.01822927572389 8 22.127359840946085 38.817173813497476 22.26157531204239 16.79389103351405 9 21.32522502545288 18.2823440405928 25.28118312825713 35.11124780569719 10-11 24.769070439437243 26.077757098144804 28.85498756361504 20.29818489880291 12-13 22.17916570124175 24.382600039475154 29.822825219473266 23.615409039809833 14-15 21.279029254520335 27.634769027452393 24.87432312986467 26.2118785881626 16-17 20.57643313632379 31.842260092419885 27.243784038725455 20.33752273253088 18-19 20.068068892721982 30.3121798007363 29.85639626187473 19.76335504466699 20-21 20.406827345355286 28.576052801945334 30.07559317402769 20.941526678671686 22-23 20.80525163512889 28.915504667334424 29.574979764700714 20.70426393283597 24-25 20.837140681842055 29.339707938896602 29.349902822506735 20.47324855675461 26-27 20.38101403735927 29.498809910388 29.513638832002737 20.60653722024999 28-29 20.343838754700116 29.165639740960707 29.879178615047376 20.6113428892918 30-31 20.54323969372785 28.851383311833683 29.816636265088942 20.788740729349524 32-33 20.58203537316891 29.088930652455613 29.50269889898652 20.82633507538896 34-35 20.459106159288705 28.85285933875367 29.87179848044745 20.816236021510175 36-37 20.27137613079109 29.080304789261113 30.044699587330335 20.60361949261746 38-39 20.184393521134304 28.614463828072385 30.24808236642128 20.953060284372032 40-41 20.304130302328176 28.294313003629995 30.222622694779837 21.178933999261986 42-43 20.330211250345837 28.108873118666384 30.292603457472918 21.268312173514857 44-45 19.72740872146548 27.93504520418258 30.88833478762032 21.449211286731618 46-47 19.645581786508078 27.79112495601953 31.395927194089023 21.167366063383362 48-49 19.26609881965903 27.8813933419515 31.51949076384736 21.333017074542106 50-51 18.821849042951012 28.117763606393737 31.321050958627993 21.73933639202726 52-53 18.69298846021558 27.874734057707844 31.543896697338276 21.888380784738292 54-55 18.46579597037509 27.69327041106348 31.71822695405503 22.122706664506403 56-57 18.362873323250582 27.817100355413555 31.685938543731247 22.134087777604623 58-59 18.22731913006406 27.81857638233354 31.503494751179623 22.450609736422784 60-61 18.322951943996106 27.35671726120802 31.445346155773702 22.87498463902217 62-63 18.366483879549296 26.844716765783627 31.638613564005524 23.150185790661553 64-65 18.72131400767191 26.72069613572587 31.026302465480697 23.531687391121523 66-67 18.932379430912672 26.92382945916314 30.353189213607184 23.790601896317003 68-69 18.8861763556964 26.709977186802536 30.3135081178048 24.090338339696267 70-71 19.273684911503604 26.392734377628102 30.34783432524631 23.985746385621987 72-73 19.738255803016997 25.937500214538794 30.371519408380948 23.952724574063254 74-75 20.187929120500776 26.350169880400625 29.712833813785817 23.74906718531278 76 20.5121058235784 26.294218162270965 29.25526546859049 23.938410545560146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 28.0 1 15.5 2 4.5 3 8.5 4 11.0 5 14.0 6 13.0 7 21.0 8 33.0 9 53.5 10 166.0 11 425.5 12 593.0 13 740.5 14 1031.0 15 1602.5 16 2389.5 17 2748.0 18 3827.0 19 6018.0 20 8322.0 21 9514.0 22 11298.0 23 15177.0 24 19567.5 25 21863.0 26 24138.0 27 29064.0 28 33982.5 29 36250.0 30 38830.5 31 43842.5 32 47701.0 33 49128.0 34 50855.0 35 54809.0 36 58866.5 37 60697.0 38 61751.0 39 64626.0 40 67294.0 41 68546.0 42 68951.0 43 69697.5 44 70164.5 45 69127.0 46 68369.0 47 66469.0 48 62700.0 49 58708.5 50 56586.0 51 54596.5 52 49622.0 53 43672.5 54 40708.0 55 38515.5 56 33405.0 57 27725.5 58 24964.0 59 22793.5 60 18540.0 61 14478.0 62 12499.0 63 10997.5 64 8381.0 65 6290.0 66 4589.5 67 3865.0 68 3341.5 69 2449.5 70 1733.5 71 1386.0 72 1163.5 73 822.0 74 578.0 75 453.0 76 369.5 77 216.0 78 115.5 79 85.0 80 65.0 81 36.5 82 18.0 83 8.0 84 12.5 85 15.5 86 12.0 87 10.0 88 10.0 89 11.5 90 10.5 91 7.5 92 7.0 93 6.5 94 8.0 95 9.5 96 9.0 97 8.0 98 12.0 99 51.0 100 85.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.865241488301971E-5 2 6.865241488301971E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.4326207441509855E-5 14-15 3.4326207441509855E-5 16-17 0.0 18-19 3.4326207441509855E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 3.4326207441509855E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.4326207441509855E-5 42-43 0.0 44-45 0.0 46-47 3.4326207441509855E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 6.865241488301971E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.4326207441509855E-5 64-65 3.4326207441509855E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1456613.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.31984686959187 #Duplication Level Percentage of deduplicated Percentage of total 1 65.55930201247989 42.167642663200326 2 22.67653016487565 29.171018954769657 3 7.903924329544506 15.251376076354328 4 2.4284367683095494 6.2478672428062705 5 0.735429949929828 2.3651370881399076 6 0.20920742951442273 0.8073713898209163 7 0.11519350758654892 0.5186460137836597 8 0.06409412844419278 0.3298019621416346 9 0.05273454591427485 0.30526901261492584 >10 0.25162717389729505 2.61597608737928 >50 0.002220828092758252 0.08851381465751577 >100 0.001299161411082329 0.13137969433160102 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.3730482976603945E-4 0.0 4.119144892981183E-4 2 1.3730482976603945E-4 0.0 4.119144892981183E-4 3 1.3730482976603945E-4 0.0 4.119144892981183E-4 4 1.3730482976603945E-4 0.0 4.119144892981183E-4 5 1.3730482976603945E-4 0.0 4.119144892981183E-4 6 1.3730482976603945E-4 0.0 4.119144892981183E-4 7 1.3730482976603945E-4 0.0 4.119144892981183E-4 8 1.3730482976603945E-4 0.0 4.119144892981183E-4 9 1.3730482976603945E-4 0.0 4.119144892981183E-4 10 1.3730482976603945E-4 0.0 5.492193190641578E-4 11 1.3730482976603945E-4 0.0 5.492193190641578E-4 12 1.3730482976603945E-4 0.0 6.178717339471774E-4 13 1.3730482976603945E-4 0.0 6.178717339471774E-4 14 1.3730482976603945E-4 2.0595724464905914E-4 7.551765637132169E-4 15 1.3730482976603945E-4 2.0595724464905914E-4 7.551765637132169E-4 16 1.3730482976603945E-4 2.0595724464905914E-4 8.238289785962366E-4 17 1.3730482976603945E-4 2.0595724464905914E-4 8.238289785962366E-4 18 1.3730482976603945E-4 2.0595724464905914E-4 0.0010297862232452957 19 1.3730482976603945E-4 2.0595724464905914E-4 0.002196877276256631 20 1.3730482976603945E-4 2.0595724464905914E-4 0.0024714869357887097 21 1.3730482976603945E-4 2.0595724464905914E-4 0.0032266634995019266 22 1.3730482976603945E-4 2.0595724464905914E-4 0.004599711797162321 23 1.3730482976603945E-4 2.0595724464905914E-4 0.005148931116226479 24 2.0595724464905914E-4 2.0595724464905914E-4 0.005972760094822715 25 2.746096595320789E-4 2.0595724464905914E-4 0.006316022169237814 26 2.746096595320789E-4 2.746096595320789E-4 0.008238289785962366 27 2.746096595320789E-4 2.746096595320789E-4 0.052931011874808205 28 2.746096595320789E-4 2.746096595320789E-4 0.12103420743876377 29 2.746096595320789E-4 2.746096595320789E-4 0.19689512588450056 30 2.746096595320789E-4 2.746096595320789E-4 0.26204626760848626 31 2.746096595320789E-4 2.746096595320789E-4 0.32012621059952096 32 2.746096595320789E-4 2.746096595320789E-4 0.3906322406843822 33 2.746096595320789E-4 2.746096595320789E-4 0.47679102136257195 34 3.432620744150986E-4 2.746096595320789E-4 0.5994042343436452 35 3.432620744150986E-4 2.746096595320789E-4 0.7647879017968396 36 3.432620744150986E-4 2.746096595320789E-4 1.040427347552164 37 3.432620744150986E-4 2.746096595320789E-4 1.4977897355028411 38 3.432620744150986E-4 2.746096595320789E-4 2.0078771780836777 39 3.432620744150986E-4 2.746096595320789E-4 2.5648542200296167 40 3.432620744150986E-4 2.746096595320789E-4 3.171055043446681 41 3.432620744150986E-4 2.746096595320789E-4 3.8070510149229753 42 3.432620744150986E-4 2.746096595320789E-4 4.468791641980403 43 4.119144892981183E-4 2.746096595320789E-4 5.17577421044574 44 4.119144892981183E-4 2.746096595320789E-4 5.979556683896134 45 4.119144892981183E-4 2.746096595320789E-4 6.8853566458626965 46 4.119144892981183E-4 2.746096595320789E-4 7.823560547654044 47 4.8056690418113804E-4 2.746096595320789E-4 8.791147683015325 48 5.492193190641578E-4 2.746096595320789E-4 9.709373732075713 49 5.492193190641578E-4 2.746096595320789E-4 10.595951017875029 50 5.492193190641578E-4 2.746096595320789E-4 11.511019055850799 51 5.492193190641578E-4 2.746096595320789E-4 12.433707511878584 52 5.492193190641578E-4 2.746096595320789E-4 13.372872547478293 53 5.492193190641578E-4 2.746096595320789E-4 14.348217405721355 54 5.492193190641578E-4 2.746096595320789E-4 15.431277902915873 55 6.178717339471774E-4 2.746096595320789E-4 16.560610127741548 56 6.178717339471774E-4 2.746096595320789E-4 17.655204230636414 57 6.178717339471774E-4 3.432620744150986E-4 18.756320312945167 58 6.178717339471774E-4 3.432620744150986E-4 19.820020829142674 59 6.178717339471774E-4 3.432620744150986E-4 20.81548084494646 60 6.865241488301972E-4 3.432620744150986E-4 21.830163536917492 61 7.551765637132169E-4 3.432620744150986E-4 22.826927948604055 62 7.551765637132169E-4 3.432620744150986E-4 23.836118447384447 63 8.238289785962366E-4 3.432620744150986E-4 24.872083387969212 64 8.924813934792563E-4 3.432620744150986E-4 25.955006580333965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAC 285 1.2732926E-11 18.421053 3 GTGTAGA 2625 0.0 18.0 70 GCTACAC 235 7.939889E-9 17.872341 3 TACACCG 275 1.6007107E-10 17.818182 5 GGGCTAA 120 0.0013413018 17.5 57 GTGCTAC 375 0.0 16.8 1 GGACGGT 190 6.1240225E-6 16.578947 6 TGCTAGG 130 0.0022911928 16.153847 2 TATACGG 265 3.4966433E-8 15.849055 5 TAGGACT 155 4.0560748E-4 15.806452 4 GAGTGTA 4025 0.0 15.652174 70 CTATACT 315 1.1077645E-9 15.555556 4 AGTGTAG 3200 0.0 15.421876 70 CTAGCAC 160 5.1633007E-4 15.312499 3 GTACACG 210 1.5821273E-5 15.000001 1 ACAGGGC 190 1.1342433E-4 14.736841 3 TGGACCG 215 1.9761588E-5 14.651163 5 GTGTAGC 335 2.6429916E-9 14.626866 1 GTTCTAC 460 0.0 14.456521 1 TCTATAC 315 1.8540959E-8 14.444445 3 >>END_MODULE