FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve10.3420000000c7a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve10.3420000000c7a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456613
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC2851.2732926E-1118.4210533
GTGTAGA26250.018.070
GCTACAC2357.939889E-917.8723413
TACACCG2751.6007107E-1017.8181825
GGGCTAA1200.001341301817.557
GTGCTAC3750.016.81
GGACGGT1906.1240225E-616.5789476
TGCTAGG1300.002291192816.1538472
TATACGG2653.4966433E-815.8490555
TAGGACT1554.0560748E-415.8064524
GAGTGTA40250.015.65217470
CTATACT3151.1077645E-915.5555564
AGTGTAG32000.015.42187670
CTAGCAC1605.1633007E-415.3124993
GTACACG2101.5821273E-515.0000011
ACAGGGC1901.1342433E-414.7368413
TGGACCG2151.9761588E-514.6511635
GTGTAGC3352.6429916E-914.6268661
GTTCTAC4600.014.4565211
TCTATAC3151.8540959E-814.4444453
Produced by FastQC (version 0.11.2)