FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve09.3420000000c787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve09.3420000000c787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432747
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG1602.6081421E-517.4999985
GGACTAC2056.6732173E-717.0731721
AGGACCG1350.00294707915.5555555
TGGACAC2755.5117198E-815.2727285
ACAGGGC1859.036819E-515.1351368
GTACAGG2352.772178E-614.8936161
GGACCGT1901.1342223E-414.7368436
GAGTGTA17100.014.32748570
GTGCTAG1750.001018160814.01
GCTACAC3051.9563777E-713.7704923
AGCGTAG2052.1629354E-413.65853770
GTATTAA7050.013.4042561
GTCTAAG2102.6526605E-413.3333331
GTGTAAG2102.6526605E-413.3333331
TAGATCG2102.6526605E-413.3333334
ACTGTGC3951.87174E-913.291148
ACCGTGC2659.612382E-613.2075478
GTGTTAC4304.765752E-1013.0232561
TAAACCG4601.0004442E-1012.9347825
GTGTATG4651.200533E-1012.7956991