##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve08.3420000000c76d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1259515 Sequences flagged as poor quality 0 Sequence length 76 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.41874610465139 34.0 34.0 34.0 34.0 34.0 2 33.580693362127484 34.0 34.0 34.0 34.0 34.0 3 33.64541510025685 34.0 34.0 34.0 34.0 34.0 4 33.65491875841098 34.0 34.0 34.0 34.0 34.0 5 33.64662588377272 34.0 34.0 34.0 34.0 34.0 6 37.42675315498426 38.0 38.0 38.0 37.0 38.0 7 37.42989087069229 38.0 38.0 38.0 37.0 38.0 8 37.382366228270406 38.0 38.0 38.0 37.0 38.0 9 37.40508449681028 38.0 38.0 38.0 37.0 38.0 10-11 37.38256670226238 38.0 38.0 38.0 37.0 38.0 12-13 37.41038177393679 38.0 38.0 38.0 37.0 38.0 14-15 37.37600584351914 38.0 38.0 38.0 37.0 38.0 16-17 37.386753234379896 38.0 38.0 38.0 37.0 38.0 18-19 37.36931080614363 38.0 38.0 38.0 37.0 38.0 20-21 37.36867246519493 38.0 38.0 38.0 37.0 38.0 22-23 37.32261465722917 38.0 38.0 38.0 37.0 38.0 24-25 37.35131657820669 38.0 38.0 38.0 37.0 38.0 26-27 37.33556884991445 38.0 38.0 38.0 37.0 38.0 28-29 37.36964387085506 38.0 38.0 38.0 37.0 38.0 30-31 37.33755175603308 38.0 38.0 38.0 37.0 38.0 32-33 37.329429978999855 38.0 38.0 38.0 37.0 38.0 34-35 37.31420149819573 38.0 38.0 38.0 37.0 38.0 36-37 37.28220743698964 38.0 38.0 38.0 37.0 38.0 38-39 37.27318293152523 38.0 38.0 38.0 37.0 38.0 40-41 37.27261961945669 38.0 38.0 38.0 37.0 38.0 42-43 37.2690773829609 38.0 38.0 38.0 37.0 38.0 44-45 37.28752892978646 38.0 38.0 38.0 37.0 38.0 46-47 37.24057315712794 38.0 38.0 38.0 37.0 38.0 48-49 37.23856682929541 38.0 38.0 38.0 37.0 38.0 50-51 37.21901446191589 38.0 38.0 38.0 37.0 38.0 52-53 37.211327376013784 38.0 38.0 38.0 37.0 38.0 54-55 37.19393615796557 38.0 38.0 38.0 37.0 38.0 56-57 37.197778509982015 38.0 38.0 38.0 37.0 38.0 58-59 37.16970540247635 38.0 38.0 38.0 37.0 38.0 60-61 37.17730356526123 38.0 38.0 38.0 37.0 38.0 62-63 37.11691127140209 38.0 38.0 38.0 37.0 38.0 64-65 37.09201954720666 38.0 38.0 38.0 37.0 38.0 66-67 37.08196686819927 38.0 38.0 38.0 37.0 38.0 68-69 37.04669456100166 38.0 38.0 38.0 36.0 38.0 70-71 37.03903526357368 38.0 38.0 38.0 36.0 38.0 72-73 36.77994902799887 38.0 38.0 38.0 35.5 38.0 74-75 36.91638130550251 38.0 38.0 38.0 36.0 38.0 76 35.92071630746756 38.0 37.0 38.0 32.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.06287557914928499 1101 2 0.01920442980066639 1101 3 -0.009554378088594717 1101 4 -0.06343473968502877 1101 5 -0.037078189702491215 1101 6 -0.04822039110939613 1101 7 -0.04606320930855645 1101 8 -0.020259975000683994 1101 9 -0.007628304617739445 1101 10-11 0.014850556006983595 1101 12-13 -0.029495036466208546 1101 14-15 -0.012493920509498935 1101 16-17 -5.013137693623548E-4 1101 18-19 0.002177167295862148 1101 20-21 -0.05001953386302915 1101 22-23 -0.03425717724103805 1101 24-25 -0.012632733991040368 1101 26-27 -0.05721260820676832 1101 28-29 -0.07509882054759487 1101 30-31 -0.06111773554278699 1101 32-33 -0.0722556772974201 1101 34-35 -0.07080290164984859 1101 36-37 -0.11212913514665246 1101 38-39 0.007479227895977658 1101 40-41 -0.08209205633966832 1101 42-43 -0.014200914683677013 1101 44-45 -0.056031553251948196 1101 46-47 0.0016466060484603418 1101 48-49 -9.765135735761987E-4 1101 50-51 -0.02299214640575542 1101 52-53 -0.027102370646957752 1101 54-55 0.07279550761152365 1101 56-57 0.0016908492897940164 1101 58-59 0.023043211523194884 1101 60-61 -0.12592165415059497 1101 62-63 -0.07550712678781935 1101 64-65 -0.042216355674462136 1101 66-67 0.00978482175004558 1101 68-69 0.057240020396960745 1101 70-71 -0.0656506297452566 1101 72-73 0.2749473477996034 1101 74-75 0.0514188155270503 1101 76 -0.04298201453670458 1102 1 -0.07882324803448881 1102 2 -0.016726813776941185 1102 3 -0.00911073305832133 1102 4 0.005388492460966177 1102 5 -0.02377080147791588 1102 6 -0.017737636072972407 1102 7 -0.031830062422329775 1102 8 0.10003832780388677 1102 9 0.032509755266247 1102 10-11 0.036335780021026665 1102 12-13 -0.022747840101033034 1102 14-15 -0.04205488150540049 1102 16-17 -0.08558753956857856 1102 18-19 -0.07692691625605619 1102 20-21 -0.036981033309437805 1102 22-23 -0.03570006381182367 1102 24-25 -0.0669794072146388 1102 26-27 0.006036091848912406 1102 28-29 -0.0739979904972472 1102 30-31 -0.07275276021489674 1102 32-33 -0.10816470329521621 1102 34-35 -0.08976851079896875 1102 36-37 -0.09915520688777235 1102 38-39 0.08211616477159112 1102 40-41 0.04991443998645906 1102 42-43 0.08975419921046779 1102 44-45 0.07969756530443561 1102 46-47 0.09320729368176472 1102 48-49 0.11269820268712039 1102 50-51 0.08112221964621824 1102 52-53 0.11456410171401643 1102 54-55 0.1517271497709416 1102 56-57 0.09877841505245755 1102 58-59 0.13942560700380824 1102 60-61 0.08928622428171451 1102 62-63 0.0592924255004732 1102 64-65 0.10939322947621832 1102 66-67 0.08007884982946223 1102 68-69 0.1545495264357868 1102 70-71 0.11343656687584769 1102 72-73 0.3934517969288507 1102 74-75 0.1993512342026591 1102 76 0.3500005746529453 1103 1 -0.027005838246630276 1103 2 -0.07057517018279214 1103 3 -0.018545854275672014 1103 4 -0.02712082831614282 1103 5 0.060118712506081806 1103 6 0.03813568199768014 1103 7 -0.0031229981615723545 1103 8 0.05258977635156015 1103 9 0.04543174171631392 1103 10-11 0.06204261086860896 1103 12-13 0.05714518201052954 1103 14-15 0.04019971651364074 1103 16-17 -0.00828635656383625 1103 18-19 0.013838521458218622 1103 20-21 -0.0074124114110603045 1103 22-23 0.04400112479988394 1103 24-25 -0.04110972264057011 1103 26-27 0.05211507338628252 1103 28-29 -0.021215743254415997 1103 30-31 0.02606696763983507 1103 32-33 -0.050574097403753626 1103 34-35 0.05482791908194429 1103 36-37 -0.038132269712136235 1103 38-39 0.03357173688902293 1103 40-41 0.006920308075571313 1103 42-43 0.06490136605962249 1103 44-45 0.025248705469515187 1103 46-47 0.028461479369035203 1103 48-49 0.03952588926961198 1103 50-51 0.04909968818741106 1103 52-53 0.09832645119244887 1103 54-55 0.06003758803331749 1103 56-57 0.03174103303477693 1103 58-59 0.06523427655501735 1103 60-61 0.010992208415920857 1103 62-63 0.02746747624657786 1103 64-65 0.06347119546152413 1103 66-67 -0.006212362873256438 1103 68-69 0.02416925038454565 1103 70-71 -0.05355583349358284 1103 72-73 0.2776635848926645 1103 74-75 0.004395360632592826 1103 76 0.2862483019457187 2116 1 -0.11115213049821904 2116 2 -0.10145394766744431 2116 3 -0.09069448704186556 2116 4 -0.09388098105490172 2116 5 -0.023800590181977554 2116 6 -0.11244667233807348 2116 7 -0.017996804574785585 2116 8 -0.0817861536751252 2116 9 -0.12094971676729216 2116 10-11 -0.15158341499810746 2116 12-13 -0.1457379322358392 2116 14-15 -0.07523677180648036 2116 16-17 -0.06227582424808986 2116 18-19 -0.08441201953357336 2116 20-21 -0.07382025743142862 2116 22-23 -0.11138190117276281 2116 24-25 -0.18511804937439535 2116 26-27 -0.08956850190111254 2116 28-29 -0.22054158425074633 2116 30-31 -0.11794293541040446 2116 32-33 -0.01573718940514368 2116 34-35 -0.0781843396155324 2116 36-37 -0.09898927511449074 2116 38-39 -0.06170587134717209 2116 40-41 -0.07509464675350586 2116 42-43 -0.11073559187668991 2116 44-45 -0.10644102375714937 2116 46-47 -0.18941240396841152 2116 48-49 -0.15810430816086551 2116 50-51 -0.28813571133943583 2116 52-53 -0.25439578420078135 2116 54-55 -0.2395694047584982 2116 56-57 -0.22675688874965516 2116 58-59 -0.40376761880784073 2116 60-61 -0.2853525975584503 2116 62-63 -0.3076881314044826 2116 64-65 -0.25769996349674784 2116 66-67 -0.2908541228998942 2116 68-69 -0.2368830921477496 2116 70-71 -0.245409619427285 2116 72-73 0.051506946961197286 2116 74-75 -0.3506016569767567 2116 76 -0.04065670796616416 1117 1 -0.06953766406007844 1117 2 -0.06717646834344038 1117 3 -0.01932063765511316 1117 4 0.02555975340584382 1117 5 -0.07191075114003809 1117 6 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66-67 0.07985156878828548 1117 68-69 0.14861726801422037 1117 70-71 0.17194386896959912 1117 72-73 0.4183811549419616 1117 74-75 0.24118837470557963 1117 76 0.5142942570076556 2117 1 0.14183754027317974 2117 2 0.02538553152486145 2117 3 0.033395878658772915 2117 4 0.027058659475464708 2117 5 0.0013584864940980879 2117 6 0.07583230224653192 2117 7 0.12620574134541584 2117 8 0.17687390839543582 2117 9 0.08117126880998882 2117 10-11 0.11711532376629208 2117 12-13 0.19458919887473058 2117 14-15 0.074966508156173 2117 16-17 0.16084333772693782 2117 18-19 0.15830893622815978 2117 20-21 0.10666357393610326 2117 22-23 0.04417722549005987 2117 24-25 0.15283100795308968 2117 26-27 0.08745005969714725 2117 28-29 0.1493222853853311 2117 30-31 0.11282327695258232 2117 32-33 0.16761540374402273 2117 34-35 0.1338764759122526 2117 36-37 0.045852681983262755 2117 38-39 0.0947071430459161 2117 40-41 0.12073942592773079 2117 42-43 0.13667835827082797 2117 44-45 0.06592977106048892 2117 46-47 0.15349088838062386 2117 48-49 0.14326113214956848 2117 50-51 0.026327414925006565 2117 52-53 0.16548532550500283 2117 54-55 0.08686355390535994 2117 56-57 0.0997623884533212 2117 58-59 0.14880742355955334 2117 60-61 0.14417148669286206 2117 62-63 0.24317480184681983 2117 64-65 0.19721745121139378 2117 66-67 0.20307095875405423 2117 68-69 0.21846508792178554 2117 70-71 0.1979205852693795 2117 72-73 0.4746191514492253 2117 74-75 0.31206486176780146 2117 76 0.6495964437045316 1116 1 0.06979642375460315 1116 2 0.019597191732344754 1116 3 0.06744767780664063 1116 4 0.03921736438380208 1116 5 -0.07407351208590995 1116 6 -0.010447343384726082 1116 7 0.0010890973249146896 1116 8 0.06810389742400247 1116 9 0.10332804390309747 1116 10-11 0.050583699402970694 1116 12-13 0.11302575151852778 1116 14-15 0.05902230833316935 1116 16-17 0.10516121676599255 1116 18-19 0.09914635438973818 1116 20-21 0.08382496502402148 1116 22-23 0.016598088254127674 1116 24-25 0.09642514703391925 1116 26-27 0.06894002714623326 1116 28-29 0.09376742321860831 1116 30-31 0.07376963843723416 1116 32-33 0.19002784183372512 1116 34-35 0.0759414556270741 1116 36-37 -0.035196872085649034 1116 38-39 0.07359498017804356 1116 40-41 0.08779736928185145 1116 42-43 0.08883319880907692 1116 44-45 0.02651592143298842 1116 46-47 0.1412809955773895 1116 48-49 0.1623472260021046 1116 50-51 0.014918438890660468 1116 52-53 0.13046897421132542 1116 54-55 0.03802481212593278 1116 56-57 0.1840859534563819 1116 58-59 0.2017654874817012 1116 60-61 0.22112648313301264 1116 62-63 0.20154462696195452 1116 64-65 0.23272743391731154 1116 66-67 0.18181253911554052 1116 68-69 0.16068497421355943 1116 70-71 0.08264204926213381 1116 72-73 0.4408655138896975 1116 74-75 0.24543808953505675 1116 76 0.5626565690216623 2118 1 -0.027235092038139896 2118 2 -0.029808303653851453 2118 3 -0.044118988754433985 2118 4 0.024583459485533865 2118 5 -0.06186949729892177 2118 6 0.015013799597319633 2118 7 0.0037325719497758314 2118 8 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70-71 0.08965913602322217 2118 72-73 0.38385028817275924 2118 74-75 0.28751906950547124 2118 76 0.5226763515249999 1119 1 0.07302909614023889 1119 2 0.07747517344454025 1119 3 0.030337515685836536 1119 4 0.0646835645242092 1119 5 0.04685507856764559 1119 6 0.11476711786449556 1119 7 0.09352790568629388 1119 8 0.03993749418334147 1119 9 0.018500822878422696 1119 10-11 0.03086089171566897 1119 12-13 0.09724930236267681 1119 14-15 -0.020038758314541383 1119 16-17 0.08046508844944356 1119 18-19 0.07492447275333092 1119 20-21 0.09451017081178037 1119 22-23 0.12958664100309392 1119 24-25 0.0024795791798197797 1119 26-27 0.06239793076505862 1119 28-29 0.0716430328987343 1119 30-31 0.07080799267826166 1119 32-33 0.09948354433458917 1119 34-35 0.1067476430367762 1119 36-37 0.08333880143968031 1119 38-39 0.1238533080471882 1119 40-41 0.1490233105273333 1119 42-43 0.13613670949640522 1119 44-45 0.096566700489781 1119 46-47 0.19100014538359744 1119 48-49 0.1395878978063081 1119 50-51 0.14624451662781723 1119 52-53 0.11412790954430108 1119 54-55 0.027972108288913944 1119 56-57 0.16738061814130134 1119 58-59 0.20309338617277461 1119 60-61 0.1142386451084576 1119 62-63 0.1277057446442953 1119 64-65 0.1092404030225893 1119 66-67 0.14593419640721805 1119 68-69 0.24335053126971928 1119 70-71 0.15076856722289023 1119 72-73 0.37256221165367975 1119 74-75 0.32911120115394255 1119 76 0.08879740063853347 2119 1 0.07887918576771114 2119 2 0.05195467273165377 2119 3 0.04926753646772397 2119 4 0.061613752470066174 2119 5 0.05842120205961976 2119 6 0.052022447838858454 2119 7 0.009991040757206804 2119 8 0.07267136346924019 2119 9 0.0733072332107838 2119 10-11 0.07115247922966006 2119 12-13 0.09971103520381774 2119 14-15 -0.02722116909483674 2119 16-17 0.13349574680768228 2119 18-19 0.0853515279977799 2119 20-21 0.12605543126703367 2119 22-23 0.14027876474012402 2119 24-25 0.1323551880907985 2119 26-27 0.12171874194849153 2119 28-29 0.041784180958131856 2119 30-31 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48-49 -0.1467164010279518 2104 50-51 0.06594780189004723 2104 52-53 -0.0076734809975533835 2104 54-55 -0.00249521136995412 2104 56-57 -0.12788500217335041 2104 58-59 0.0016683880818320063 2104 60-61 -0.09080267443493284 2104 62-63 -0.07955688449673914 2104 64-65 -0.09482710884618228 2104 66-67 -0.05858711337667444 2104 68-69 -0.34250071464908416 2104 70-71 -0.19332311988470252 2104 72-73 0.02421080276619847 2104 74-75 0.06846226920406906 2104 76 -0.21548951362876778 2111 1 0.01653159247526048 2111 2 0.06828418611547704 2111 3 0.09158895878365314 2111 4 0.07056073672271168 2111 5 0.1036191600400187 2111 6 0.16184519287821075 2111 7 0.030995020047605237 2111 8 0.15214721086890393 2111 9 0.03299287604672685 2111 10-11 0.11039033091110895 2111 12-13 0.0563541994083252 2111 14-15 0.05506835895420892 2111 16-17 0.09409973148154904 2111 18-19 0.16790245082108157 2111 20-21 0.09195856620028309 2111 22-23 0.017222915219527124 2111 24-25 0.04588692309634723 2111 26-27 0.0692123731652785 2111 28-29 0.06303046466133111 2111 30-31 0.06503300549267976 2111 32-33 0.0602913204016744 2111 34-35 0.08182634673610778 2111 36-37 0.018222310524947716 2111 38-39 0.034400122942692235 2111 40-41 0.06174045957963159 2111 42-43 0.07520716098896685 2111 44-45 -0.05810350859066915 2111 46-47 0.07048488748561255 2111 48-49 -0.08444058939295473 2111 50-51 0.052097334338256474 2111 52-53 0.0434305121706231 2111 54-55 0.07631831583257309 2111 56-57 -0.015103124293943893 2111 58-59 0.09287757520135642 2111 60-61 -0.09852150188692832 2111 62-63 0.009038501525147069 2111 64-65 0.058639706182162854 2111 66-67 0.05641903951428162 2111 68-69 -0.21359337694595837 2111 70-71 -0.04504699189668315 2111 72-73 0.10828189149599865 2111 74-75 0.14965397584754925 2111 76 -0.2914446905246564 2110 1 0.0655152645845618 2110 2 0.013968957858232045 2110 3 0.05460128800724107 2110 4 -0.00907938323729951 2110 5 0.04563848693105399 2110 6 0.12452429984255531 2110 7 0.07298102471271761 2110 8 -0.08077514834469213 2110 9 0.008334428862454502 2110 10-11 0.05140999102440702 2110 12-13 0.005037971484291859 2110 14-15 0.11104969850769209 2110 16-17 -0.0037010016074106034 2110 18-19 0.016786156252599937 2110 20-21 0.006153834444205586 2110 22-23 -0.004103309372275987 2110 24-25 -0.0015972488463376067 2110 26-27 0.034224035944347975 2110 28-29 0.029874849866260433 2110 30-31 0.0570356712707607 2110 32-33 -0.02384730005817204 2110 34-35 -0.017807108778718828 2110 36-37 0.06337392665290054 2110 38-39 -0.011751159963004909 2110 40-41 -0.029395828324403794 2110 42-43 -0.035256017951390106 2110 44-45 0.04286283596115226 2110 46-47 0.08647955592946488 2110 48-49 0.0401845355653947 2110 50-51 0.07525628135591234 2110 52-53 0.14223091203733418 2110 54-55 0.01633830916812684 2110 56-57 -0.05558962879243978 2110 58-59 -0.002257379813634941 2110 60-61 -0.018214937408416176 2110 62-63 -0.03611311460280575 2110 64-65 -0.15728831780983654 2110 66-67 -0.03721641533410036 2110 68-69 -0.03881830197094871 2110 70-71 0.04092435098902314 2110 72-73 0.2590649638052227 2110 74-75 0.08600852433138328 2110 76 0.012787232167781326 2109 1 0.1266998626861806 2109 2 0.0056446112152244154 2109 3 -0.005238001381059121 2109 4 -0.032540151823113206 2109 5 0.01540653424392957 2109 6 -0.09245787346957712 2109 7 0.011373337448603138 2109 8 -0.5278352234907615 2109 9 -0.07530154620633311 2109 10-11 -0.08105405869929427 2109 12-13 -0.21616425552770124 2109 14-15 -0.14575663562097674 2109 16-17 -0.12684765783217955 2109 18-19 -0.12845932216504963 2109 20-21 -0.2061911715102056 2109 22-23 0.018671857930137037 2109 24-25 -0.051768816129822426 2109 26-27 0.04330709592763782 2109 28-29 -0.02253864474477041 2109 30-31 -0.016825432693181597 2109 32-33 -0.16381974712339797 2109 34-35 -0.020415650891493442 2109 36-37 0.006023903617915494 2109 38-39 0.0553018523709099 2109 40-41 -0.07380841512739522 2109 42-43 -0.1545394518372305 2109 44-45 -0.17221385436851477 2109 46-47 -0.02849758952275039 2109 48-49 -0.3921911469895534 2109 50-51 -0.21986932375180857 2109 52-53 -0.29902806117881653 2109 54-55 -0.18261904728736766 2109 56-57 -0.32644794793687737 2109 58-59 -0.14462319224090692 2109 60-61 -0.325820442558836 2109 62-63 -0.2683265563481996 2109 64-65 -0.3176610205798056 2109 66-67 -0.048545210564569174 2109 68-69 -0.487397268382054 2109 70-71 -0.07113709969478776 2109 72-73 0.22983730598277674 2109 74-75 -0.015630474577086773 2109 76 -0.8732492320019247 2108 1 0.23995865260376803 2108 2 0.18021677327496377 2108 3 0.07804893348430397 2108 4 0.07592520977647865 2108 5 0.09459562899685636 2108 6 0.18150162144551274 2108 7 0.16937904316411334 2108 8 -0.0909441385805323 2108 9 0.10699155421187356 2108 10-11 0.14198804259660136 2108 12-13 0.017989177207219598 2108 14-15 0.14296755166709119 2108 16-17 0.09476691294570116 2108 18-19 0.1066330963252895 2108 20-21 0.10489908621124755 2108 22-23 0.21692026392242525 2108 24-25 0.14699580521980238 2108 26-27 0.14996466693932575 2108 28-29 0.15886471968849492 2108 30-31 0.166522520045703 2108 32-33 0.11338764054953288 2108 34-35 0.20161158112105682 2108 36-37 0.07423036435832842 2108 38-39 0.10475263966736748 2108 40-41 0.06701281442682472 2108 42-43 0.12198472487508383 2108 44-45 0.19311886930450584 2108 46-47 0.16185018125118944 2108 48-49 0.07856939388697981 2108 50-51 0.1578272955884188 2108 52-53 0.1007336865763051 2108 54-55 0.1896724097437712 2108 56-57 0.14957149639091227 2108 58-59 0.20676018196973445 2108 60-61 0.05913014955301321 2108 62-63 0.2120139073150611 2108 64-65 0.07912519860862943 2108 66-67 0.22544649110831472 2108 68-69 0.08046237029052605 2108 70-71 0.1725648914993556 2108 72-73 0.525939821712889 2108 74-75 0.27035762875204483 2108 76 0.020845487230317872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 15.0 13 91.0 14 345.0 15 566.0 16 816.0 17 1066.0 18 1244.0 19 1352.0 20 1281.0 21 1246.0 22 1223.0 23 1330.0 24 1452.0 25 1638.0 26 2043.0 27 2450.0 28 2928.0 29 3923.0 30 5430.0 31 7523.0 32 10234.0 33 15413.0 34 24587.0 35 43836.0 36 105249.0 37 1022229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63570898321973 25.905685918786197 8.037300071853053 24.421305026141017 2 17.03965415259048 23.9432638753806 37.57922692464957 21.437855047379347 3 20.0342195210061 25.808346863673716 30.937225836929294 23.22020777839089 4 14.407291695612995 16.559628110820434 39.379364279107435 29.653715914459138 5 14.370452118474175 33.216436485472585 36.469355267702255 15.94375612835099 6 26.937908639436607 36.00036521994577 20.479867250489274 16.58185889012834 7 23.137477521109314 33.61841661274379 23.677844249572257 19.566261616574636 8 21.14679062972652 38.657181534161964 22.880156250620278 17.31587158549124 9 20.957590818688146 19.199691944915305 24.35921763535964 35.483499601036904 10-11 23.989472138084896 25.952410253153 29.264002413627466 20.794115195134637 12-13 21.728522486830247 24.872986824293477 29.423746442082866 23.97474424679341 14-15 21.285097835277863 28.4483313021282 24.800339813340848 25.466231049253086 16-17 20.596777330956755 31.80057403047999 27.084274502487066 20.518374136076186 18-19 20.133662560588796 30.411110625915533 29.533987288757974 19.921239524737697 20-21 20.41186488449919 28.654640873669628 29.822391952457888 21.11110228937329 22-23 20.771765322366146 28.90430046486148 29.3393488763532 20.984585336419176 24-25 20.760014767589112 29.510644970484673 29.1725783337237 20.556761928202523 26-27 20.429609810125328 29.726164436310803 29.265550628614985 20.57867512494889 28-29 20.30158434000389 29.48650869580751 29.686149033556568 20.52575793063203 30-31 20.389197429169165 29.085600409681504 29.909250783039504 20.615951378109827 32-33 20.549298738006296 29.315887464619315 29.562887301858254 20.57192649551613 34-35 20.526432793575307 29.04526742436573 29.742083262208073 20.686216519850895 36-37 20.338781197524444 29.30532784444806 29.89857206940767 20.457318888619827 38-39 20.265379927988153 28.97559774992755 29.996506591823042 20.762515730261253 40-41 20.431396212034 28.87055731769769 29.84906888762738 20.848977582640938 42-43 20.550449974791885 28.830343425842486 29.88312961735271 20.736076982012918 44-45 20.07558464964689 28.611767227861517 30.385346740610476 20.927301381881122 46-47 20.059229147727496 28.428442694211657 30.68764564137783 20.82468251668301 48-49 19.78729907940755 28.389896110804557 30.90649972410015 20.916305085687746 50-51 19.619178810891494 28.69699844781523 30.622580914082008 21.06124182721127 52-53 19.527953220088683 28.52641691444723 30.773353235173857 21.17227663029023 54-55 19.362969079367854 28.362226730130246 30.970095632048846 21.304708558453058 56-57 19.16261418085533 28.364092527679308 31.066799522038245 21.406493769427122 58-59 19.024267277483794 28.335827679702106 31.097446239227004 21.542458803587095 60-61 19.15745346423028 28.165524031075456 31.015629031809866 21.661393472884406 62-63 19.04796687613883 27.804313565142134 31.15353925915928 21.99418029955975 64-65 19.29659432400567 27.626824611060606 30.817298722127166 22.259282342806557 66-67 19.455822280798564 27.72209937952307 30.373596185833435 22.44848215384493 68-69 19.247646911708078 27.67648658412167 30.345728316058164 22.73013818811209 70-71 19.53934649448399 27.51043060225563 30.449458720221674 22.50076418303871 72-73 19.714413881533766 27.187687324089033 30.53540450093885 22.56249429343835 74-75 20.04882831883701 27.359658281163778 30.04362790439177 22.547885495607435 76 20.277805345708465 27.15712000254066 29.621481284462668 22.9435933672882 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29.0 1 16.0 2 6.5 3 8.0 4 6.0 5 7.0 6 8.5 7 15.5 8 22.0 9 43.5 10 118.0 11 287.5 12 404.0 13 578.0 14 889.0 15 1348.0 16 2060.5 17 2451.0 18 3385.0 19 5385.5 20 7507.5 21 8563.0 22 9789.5 23 13246.5 24 17310.0 25 19143.0 26 21302.0 27 25306.5 28 29188.5 29 31225.0 30 33576.5 31 37602.0 32 40847.5 33 42419.0 34 43906.5 35 47461.5 36 51520.0 37 53511.0 38 54608.5 39 57127.5 40 59364.0 41 60104.0 42 60029.0 43 60788.5 44 61049.0 45 60192.0 46 59834.0 47 58337.5 48 55267.5 49 52389.0 50 51084.0 51 49025.5 52 44583.5 53 39452.5 54 36705.0 55 34132.0 56 28967.0 57 23747.0 58 21119.0 59 18807.0 60 14359.5 61 10601.0 62 8978.0 63 7735.0 64 5455.0 65 3662.0 66 2455.5 67 2005.0 68 1619.5 69 1033.5 70 661.5 71 490.0 72 413.5 73 271.0 74 154.0 75 103.0 76 86.0 77 50.5 78 26.0 79 20.0 80 23.0 81 20.5 82 13.0 83 11.0 84 11.0 85 8.5 86 7.5 87 9.0 88 10.0 89 8.5 90 6.5 91 8.0 92 9.0 93 7.5 94 3.5 95 3.5 96 6.0 97 7.5 98 8.0 99 55.5 100 104.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1259515.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.31256293941979 #Duplication Level Percentage of deduplicated Percentage of total 1 95.49868254042558 86.24730777563856 2 3.179662545459885 5.743269475259232 3 0.577869160743423 1.5656653485117027 4 0.19478471535813613 0.7036602746167455 5 0.1004948787543352 0.453797503129513 6 0.09801500897765529 0.5311192000381374 7 0.05217368082223045 0.32983571831272207 8 0.05636588115771051 0.407243775175327 9 0.029843245825006567 0.24256980151987342 >10 0.20445210786259835 3.1661880188646587 >50 0.00619408723351472 0.36594460794765704 >100 0.0014621473799038215 0.24339850098579074 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.969782019269322E-4 0.0 0.0 2 3.969782019269322E-4 0.0 0.0 3 3.969782019269322E-4 7.939564038538644E-5 0.0 4 3.969782019269322E-4 7.939564038538644E-5 0.0 5 3.969782019269322E-4 7.939564038538644E-5 0.0 6 3.969782019269322E-4 7.939564038538644E-5 0.0 7 3.969782019269322E-4 1.5879128077077287E-4 0.0 8 3.969782019269322E-4 1.5879128077077287E-4 0.0 9 3.969782019269322E-4 1.5879128077077287E-4 0.0 10 4.763738423123186E-4 1.5879128077077287E-4 0.0 11 4.763738423123186E-4 1.5879128077077287E-4 0.0 12 4.763738423123186E-4 1.5879128077077287E-4 7.939564038538644E-5 13 4.763738423123186E-4 2.381869211561593E-4 7.939564038538644E-5 14 4.763738423123186E-4 2.381869211561593E-4 7.939564038538644E-5 15 4.763738423123186E-4 2.381869211561593E-4 7.939564038538644E-5 16 4.763738423123186E-4 2.381869211561593E-4 7.939564038538644E-5 17 5.557694826977051E-4 2.381869211561593E-4 7.939564038538644E-5 18 5.557694826977051E-4 2.381869211561593E-4 1.5879128077077287E-4 19 6.351651230830915E-4 2.381869211561593E-4 3.969782019269322E-4 20 6.351651230830915E-4 3.1758256154154575E-4 3.969782019269322E-4 21 7.145607634684779E-4 3.1758256154154575E-4 8.733520442392508E-4 22 8.733520442392508E-4 3.1758256154154575E-4 0.0011909346057807967 23 8.733520442392508E-4 3.1758256154154575E-4 0.0016673084480931153 24 8.733520442392508E-4 3.1758256154154575E-4 0.0020642866500200476 25 8.733520442392508E-4 3.1758256154154575E-4 0.002699451773103139 26 9.527476846246372E-4 3.1758256154154575E-4 0.004128573300040095 27 9.527476846246372E-4 3.1758256154154575E-4 0.017308249604014243 28 9.527476846246372E-4 3.1758256154154575E-4 0.039300841990766285 29 0.0010321433250100238 3.1758256154154575E-4 0.06510442511601688 30 0.0010321433250100238 3.1758256154154575E-4 0.0959893292259322 31 0.0010321433250100238 3.969782019269322E-4 0.12274566003580743 32 0.0010321433250100238 3.969782019269322E-4 0.14894622136298497 33 0.0010321433250100238 3.969782019269322E-4 0.18221299468446187 34 0.0010321433250100238 3.969782019269322E-4 0.23040614839839144 35 0.0010321433250100238 3.969782019269322E-4 0.2980512340067407 36 0.0010321433250100238 3.969782019269322E-4 0.42452848914066127 37 0.0011909346057807967 3.969782019269322E-4 0.6261934157195428 38 0.0011909346057807967 3.969782019269322E-4 0.8611251156199013 39 0.0011909346057807967 3.969782019269322E-4 1.1254332024628528 40 0.0011909346057807967 3.969782019269322E-4 1.4444448855313354 41 0.0011909346057807967 3.969782019269322E-4 1.7956911985962851 42 0.0011909346057807967 3.969782019269322E-4 2.161228726930604 43 0.0011909346057807967 3.969782019269322E-4 2.541851426938147 44 0.0011909346057807967 3.969782019269322E-4 2.986467013096311 45 0.0011909346057807967 3.969782019269322E-4 3.4998392238282197 46 0.0011909346057807967 3.969782019269322E-4 4.032186992612235 47 0.001270330246166183 3.969782019269322E-4 4.568504543415521 48 0.001270330246166183 3.969782019269322E-4 5.097120717101424 49 0.001270330246166183 3.969782019269322E-4 5.603109133277492 50 0.0013497258865515695 3.969782019269322E-4 6.136092067184591 51 0.0013497258865515695 3.969782019269322E-4 6.704564852343958 52 0.0013497258865515695 3.969782019269322E-4 7.296935725259326 53 0.0013497258865515695 3.969782019269322E-4 7.910902212359519 54 0.0014291215269369559 3.969782019269322E-4 8.569012675513987 55 0.0014291215269369559 3.969782019269322E-4 9.264518485289972 56 0.0014291215269369559 3.969782019269322E-4 9.982731448216178 57 0.0014291215269369559 3.969782019269322E-4 10.674743849815206 58 0.0014291215269369559 3.969782019269322E-4 11.3338070606543 59 0.0014291215269369559 3.969782019269322E-4 11.970560096545098 60 0.0014291215269369559 3.969782019269322E-4 12.601358459406994 61 0.0015085171673223424 3.969782019269322E-4 13.225805171038058 62 0.0015085171673223424 3.969782019269322E-4 13.892252176432992 63 0.0015879128077077287 3.969782019269322E-4 14.578548091924272 64 0.0017467040884785016 3.969782019269322E-4 15.328995684846944 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 280 0.0 20.0 3 CTCTAGC 175 2.7966144E-6 18.0 2 CTCCTAG 175 5.621546E-5 16.0 1 GTGTAGA 1145 0.0 15.58952 70 AGTGTAG 1330 0.0 15.526317 70 TAGGTTG 250 3.1172385E-7 15.4 5 CTAGTAC 160 5.162611E-4 15.3125 3 GTATAGA 300 9.740688E-9 15.166668 1 ACCTCGC 190 1.1340501E-4 14.736841 8 ACAGGGC 215 1.975738E-5 14.651163 8 GTATGGG 215 1.975738E-5 14.651163 1 ACACCGT 265 6.0303137E-7 14.528302 6 GTCTAGA 270 7.4482523E-7 14.25926 1 TACACCA 470 0.0 14.148936 5 GTGTACG 225 3.0335388E-5 14.0 1 GTCTTAC 225 3.0335388E-5 14.0 1 TACACAG 325 2.795241E-8 14.0 5 AGCGTCC 200 1.7539393E-4 13.999999 8 GGCCATA 200 1.7539393E-4 13.999999 1 GAGTGTA 1645 0.0 13.829786 70 >>END_MODULE