FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve08.3420000000c76d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve08.3420000000c76d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259515
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC2800.020.03
CTCTAGC1752.7966144E-618.02
CTCCTAG1755.621546E-516.01
GTGTAGA11450.015.5895270
AGTGTAG13300.015.52631770
TAGGTTG2503.1172385E-715.45
CTAGTAC1605.162611E-415.31253
GTATAGA3009.740688E-915.1666681
ACCTCGC1901.1340501E-414.7368418
ACAGGGC2151.975738E-514.6511638
GTATGGG2151.975738E-514.6511631
ACACCGT2656.0303137E-714.5283026
GTCTAGA2707.4482523E-714.259261
TACACCA4700.014.1489365
GTGTACG2253.0335388E-514.01
GTCTTAC2253.0335388E-514.01
TACACAG3252.795241E-814.05
AGCGTCC2001.7539393E-413.9999998
GGCCATA2001.7539393E-413.9999991
GAGTGTA16450.013.82978670
Produced by FastQC (version 0.11.2)