FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve19.3410000000c8cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve19.3410000000c8cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440329
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA400.002417817735.00000457
ACAGGCC703.4860044E-530.03
GTGTAGC755.5803568E-528.01
GGTTGGG1101.0145195E-625.45454867
GTTATAG750.001831243723.3333343
TGGGGTC1403.2774005E-722.566
AGGACTG1102.9181752E-522.2727285
GTGGGTT1154.1120933E-521.30434864
GGGTTGG1356.010765E-620.7407466
GGTAGGA1709.287942E-820.58823611
GGGGTCT1558.7240915E-720.3225867
CTTATAG1055.459208E-420.03
CTCCGTG900.005271798519.4444437
CGGTAGG1801.6968988E-719.44444310
GTGTAAG1107.467107E-419.090911
AATTACG1107.467107E-419.090915
GATAGTG1501.4930549E-518.66666660
CGGTAGT950.00720371418.42105351
ACGGTAG1902.996585E-718.4210539
GTACAGA1351.4099371E-418.1481481