##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve19.3410000000c8cc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 440329 Sequences flagged as poor quality 0 Sequence length 76 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.63572465134025 34.0 34.0 34.0 34.0 34.0 2 33.72108128240475 34.0 34.0 34.0 34.0 34.0 3 33.745333602828794 34.0 34.0 34.0 34.0 34.0 4 33.77175475610282 34.0 34.0 34.0 34.0 34.0 5 33.773455756945374 34.0 34.0 34.0 34.0 34.0 6 37.500230509459975 38.0 38.0 38.0 37.0 38.0 7 37.54409089567119 38.0 38.0 38.0 37.0 38.0 8 37.39417117655208 38.0 38.0 38.0 37.0 38.0 9 37.38550265823964 38.0 38.0 38.0 37.0 38.0 10-11 37.37815928544339 38.0 38.0 38.0 37.0 38.0 12-13 37.305412543802476 38.0 38.0 38.0 37.0 38.0 14-15 37.36732193428096 38.0 38.0 38.0 37.0 38.0 16-17 37.300890924740365 38.0 38.0 38.0 37.0 38.0 18-19 37.32114282729505 38.0 38.0 38.0 37.0 38.0 20-21 37.25891549273384 38.0 38.0 38.0 36.5 38.0 22-23 37.32433021672431 38.0 38.0 38.0 37.0 38.0 24-25 37.2328440779508 38.0 38.0 38.0 36.0 38.0 26-27 37.186144905286724 38.0 38.0 38.0 36.0 38.0 28-29 37.10114936785904 38.0 38.0 38.0 36.0 38.0 30-31 37.13030597575903 38.0 38.0 38.0 36.0 38.0 32-33 37.06828303382244 38.0 38.0 38.0 36.0 38.0 34-35 37.1151457205862 38.0 38.0 38.0 36.0 38.0 36-37 37.00186451494224 38.0 38.0 38.0 36.0 38.0 38-39 37.06709074351224 38.0 38.0 38.0 36.0 38.0 40-41 35.93782944116785 38.0 37.5 38.0 32.5 38.0 42-43 36.5235687406462 38.0 37.0 38.0 34.0 38.0 44-45 36.71643475673871 38.0 37.0 38.0 34.5 38.0 46-47 36.25029125949006 38.0 37.0 38.0 33.0 38.0 48-49 36.59766788015325 38.0 37.0 38.0 34.0 38.0 50-51 36.6686897751454 38.0 37.0 38.0 34.5 38.0 52-53 36.68631636798848 38.0 37.0 38.0 34.5 38.0 54-55 36.652880005632156 38.0 37.0 38.0 34.0 38.0 56-57 36.624639757999134 38.0 37.0 38.0 34.0 38.0 58-59 36.584902425232045 38.0 37.0 38.0 34.0 38.0 60-61 36.575628677647856 38.0 37.0 38.0 34.0 38.0 62-63 36.58312648042714 38.0 37.0 38.0 34.0 38.0 64-65 36.48240860810894 38.0 37.0 38.0 34.0 38.0 66-67 36.4527058176954 38.0 37.0 38.0 34.0 38.0 68-69 36.43298874250844 38.0 37.0 38.0 34.0 38.0 70-71 36.325901768904615 38.0 37.0 38.0 34.0 38.0 72-73 36.379114253206126 38.0 37.0 38.0 34.0 38.0 74-75 36.3655187371261 38.0 37.0 38.0 34.0 38.0 76 35.51848958392475 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18361803349885975 1101 2 0.0440440607626158 1101 3 0.1184075561577842 1101 4 0.06221193843403228 1101 5 0.06346019978430206 1101 6 0.15440607793505023 1101 7 0.09001538336643478 1101 8 0.19553689431747756 1101 9 0.18454117170721673 1101 10-11 0.15943408120185865 1101 12-13 0.27028469974605684 1101 14-15 0.20376580951973722 1101 16-17 0.2915940442995151 1101 18-19 0.19399082763993647 1101 20-21 0.28688431275313064 1101 22-23 0.16376781391296902 1101 24-25 0.18956608687047094 1101 26-27 0.18103004864819638 1101 28-29 0.21154966022882604 1101 30-31 0.31402165668017545 1101 32-33 0.3830074301423423 1101 34-35 0.2086062167833873 1101 36-37 0.2488129820029954 1101 38-39 0.23625083669209346 1101 40-41 1.477766525052452 1101 42-43 0.8630011078053883 1101 44-45 0.4422298855378983 1101 46-47 0.8809880422389469 1101 48-49 0.5808015126588941 1101 50-51 0.4617882565335094 1101 52-53 0.428260699565179 1101 54-55 0.37707884740624564 1101 56-57 0.34293367391983054 1101 58-59 0.4131876072177647 1101 60-61 0.4506069349041226 1101 62-63 0.3726527893270841 1101 64-65 0.49690609196019864 1101 66-67 0.46414162906859247 1101 68-69 0.5362284537864355 1101 70-71 0.5499021029957589 1101 72-73 0.45953528381355824 1101 74-75 0.5977965603993027 1101 76 0.5153414594252723 1102 1 0.10817502181349425 1102 2 0.13846037263306954 1102 3 0.08730358508716307 1102 4 0.10627304686423145 1102 5 0.12401154393764813 1102 6 0.06418134991155 1102 7 0.19355397928968898 1102 8 0.15837155826386606 1102 9 0.19736270259434008 1102 10-11 0.26294457848667463 1102 12-13 0.23315570867043078 1102 14-15 0.16032042777530364 1102 16-17 0.2221021409832673 1102 18-19 0.2567582991900679 1102 20-21 0.28446966236738547 1102 22-23 0.18318275139349538 1102 24-25 0.24576794504564958 1102 26-27 0.3076431242885107 1102 28-29 0.2485222762731496 1102 30-31 0.3509893860047626 1102 32-33 0.4294043906026701 1102 34-35 0.35364772335907446 1102 36-37 0.18388466407301252 1102 38-39 0.229532207905649 1102 40-41 1.4596059474421779 1102 42-43 0.8751213944114937 1102 44-45 0.5814155747791716 1102 46-47 1.045989050124895 1102 48-49 0.6413537730721899 1102 50-51 0.6259337830470102 1102 52-53 0.6234075760350137 1102 54-55 0.647889554209172 1102 56-57 0.5630419147618113 1102 58-59 0.7107524936931569 1102 60-61 0.5960735860961819 1102 62-63 0.5660358653949871 1102 64-65 0.6464479619856718 1102 66-67 0.7298451029154833 1102 68-69 0.47434547850712505 1102 70-71 0.7123027651462763 1102 72-73 0.6979800241760401 1102 74-75 0.7467740142961574 1102 76 0.786007702576228 1103 1 0.035117826907558936 1103 2 -0.02095164392180493 1103 3 0.03250124457053971 1103 4 0.039348374897542726 1103 5 0.07725158121129994 1103 6 0.1586918813968694 1103 7 0.12128171374011032 1103 8 0.164588030866895 1103 9 0.2648918121402559 1103 10-11 0.1362771282898123 1103 12-13 0.23436248508728852 1103 14-15 0.15875476654577625 1103 16-17 0.18875943327080336 1103 18-19 0.23491879415728079 1103 20-21 0.15325214039356183 1103 22-23 0.14320172401751563 1103 24-25 0.19221015829458565 1103 26-27 0.23312940271596716 1103 28-29 0.229677675495779 1103 30-31 0.19749791948809303 1103 32-33 0.3038954457659955 1103 34-35 0.2611939586364045 1103 36-37 0.23125955808655618 1103 38-39 0.2072855470035222 1103 40-41 1.3343383578888748 1103 42-43 0.7533691970290448 1103 44-45 0.46141582662816205 1103 46-47 0.9458140150759107 1103 48-49 0.5494142000401752 1103 50-51 0.38352493139446864 1103 52-53 0.319446830897796 1103 54-55 0.36254254827392884 1103 56-57 0.3412301131180726 1103 58-59 0.4814670732312649 1103 60-61 0.4761095165522775 1103 62-63 0.48187003122914973 1103 64-65 0.48205143414376295 1103 66-67 0.51896144875451 1103 68-69 0.4998116090152749 1103 70-71 0.49649126572133184 1103 72-73 0.5873784408853737 1103 74-75 0.5291366096839667 1103 76 0.8082165861296531 2116 1 0.012481895064468063 2116 2 0.01977393484725809 2116 3 0.02868983858522256 2116 4 -0.022796641211662916 2116 5 0.0778286895249849 2116 6 -0.024882536512585318 2116 7 0.1289252850026088 2116 8 0.20888005597733894 2116 9 0.22745450520167054 2116 10-11 0.17333038832899916 2116 12-13 0.14824256281008275 2116 14-15 0.06853488412640729 2116 16-17 0.22966561787903572 2116 18-19 0.1474142271130745 2116 20-21 0.24564552067057832 2116 22-23 0.20380847659996704 2116 24-25 0.19286200005895893 2116 26-27 0.22913739089147356 2116 28-29 0.17944849291365017 2116 30-31 0.21250481157299106 2116 32-33 0.2512083630277644 2116 34-35 0.18461915875597157 2116 36-37 0.19320570017976024 2116 38-39 0.17739839451407136 2116 40-41 1.38632170063309 2116 42-43 0.6134370383948351 2116 44-45 0.5521982858569174 2116 46-47 0.869085596435724 2116 48-49 0.47253423856942334 2116 50-51 0.4681501846578229 2116 52-53 0.4649030370956879 2116 54-55 0.3568897931007129 2116 56-57 0.25493581278924893 2116 58-59 0.4435523025799242 2116 60-61 0.5364360186946371 2116 62-63 0.496727456412664 2116 64-65 0.5334991871580215 2116 66-67 0.5607312199040848 2116 68-69 0.498750809201276 2116 70-71 0.54918250031794 2116 72-73 0.591605032707875 2116 74-75 0.40434005074418167 2116 76 0.6885143574120747 1117 1 -0.04928107234864143 1117 2 0.05204712018460356 1117 3 0.0650194284147716 1117 4 -0.016968209316623017 1117 5 0.01860324597307539 1117 6 0.0077007233395463 1117 7 0.08914490496753302 1117 8 0.1306492248807345 1117 9 0.2537514887580343 1117 10-11 0.21879566967317743 1117 12-13 0.14913826634008842 1117 14-15 0.12707113862006025 1117 16-17 0.21145661312132802 1117 18-19 0.2992877554703597 1117 20-21 0.21092141431501688 1117 22-23 0.22377376278905103 1117 24-25 0.18547471699000084 1117 26-27 0.29650410729926335 1117 28-29 0.37055613721942393 1117 30-31 0.2837983310156318 1117 32-33 0.2447525147317151 1117 34-35 0.2436259755503869 1117 36-37 0.37293324642446635 1117 38-39 0.38586233866189446 1117 40-41 1.561058985155526 1117 42-43 0.6979765535158151 1117 44-45 0.3368170153265879 1117 46-47 0.9101068801442196 1117 48-49 0.6630544370171165 1117 50-51 0.41249483732591585 1117 52-53 0.3703860266016008 1117 54-55 0.45937487076469097 1117 56-57 0.34905008978269336 1117 58-59 0.4037416418305142 1117 60-61 0.4934713187401769 1117 62-63 0.252677616779323 1117 64-65 0.48315265466317925 1117 66-67 0.5156959247034507 1117 68-69 0.45843688297963325 1117 70-71 0.3248060407875073 1117 72-73 0.3174870493550017 1117 74-75 0.3908324296939938 1117 76 0.6221693123059211 2117 1 0.05065834748545228 2117 2 0.09342017210747144 2117 3 0.016070161841113872 2117 4 0.07716561128481914 2117 5 0.015283649890101003 2117 6 0.06959345950676976 2117 7 0.15023975602282036 2117 8 0.08494813874731477 2117 9 0.1887761825634655 2117 10-11 -0.01017180788938532 2117 12-13 -0.03544408143685729 2117 14-15 0.012814421547908239 2117 16-17 0.1499862142895978 2117 18-19 0.21722995066408401 2117 20-21 0.168647047728669 2117 22-23 0.2127542059846803 2117 24-25 0.14460095891418234 2117 26-27 0.3035450124555936 2117 28-29 0.29132694595232067 2117 30-31 0.3168015927404113 2117 32-33 0.2488663020140649 2117 34-35 0.16470091641700435 2117 36-37 0.3401571603218301 2117 38-39 0.22386857398628734 2117 40-41 1.4341087532393786 2117 42-43 0.8114076811318753 2117 44-45 0.6025804793300296 2117 46-47 1.0321183509740095 2117 48-49 0.6740844599070641 2117 50-51 0.470339968810741 2117 52-53 0.4182591333175054 2117 54-55 0.5570480752461435 2117 56-57 0.5469963975323964 2117 58-59 0.6664169949377694 2117 60-61 0.643750455398937 2117 62-63 0.5491728941121252 2117 64-65 -0.021477049072721854 2117 66-67 0.3540829408470785 2117 68-69 0.5844741028425418 2117 70-71 0.6537387955720533 2117 72-73 0.6157033821124926 2117 74-75 0.7647514985325259 2117 76 0.9618448882792379 1116 1 0.048084736159296426 1116 2 0.01561705054162843 1116 3 0.08046205568924591 1116 4 0.08911852219091543 1116 5 0.12308139639095828 1116 6 0.1024751363256371 1116 7 0.06168083989775397 1116 8 0.11479107859316429 1116 9 0.11105802982350355 1116 10-11 0.07693760300600871 1116 12-13 -0.008450429552254946 1116 14-15 0.08321833187621053 1116 16-17 0.21482057543101973 1116 18-19 0.17756618804306612 1116 20-21 0.3037855595028347 1116 22-23 0.15719057314567664 1116 24-25 0.15445199282744682 1116 26-27 0.2517823184277077 1116 28-29 0.31461595702847944 1116 30-31 0.18400161187413744 1116 32-33 0.2446430366683856 1116 34-35 0.2518691760918088 1116 36-37 0.42180276101365877 1116 38-39 0.33815416239225726 1116 40-41 1.4654616205910855 1116 42-43 0.6051845643242899 1116 44-45 0.4013425150068599 1116 46-47 0.9211902899877842 1116 48-49 0.5561858082049724 1116 50-51 0.4228411363453475 1116 52-53 0.3729562729521305 1116 54-55 0.4484793153252511 1116 56-57 0.34720762373954983 1116 58-59 0.5307044715398703 1116 60-61 0.4573895883896242 1116 62-63 0.4949140047994405 1116 64-65 -0.11941211778418648 1116 66-67 0.2869408893345735 1116 68-69 0.44303530997891727 1116 70-71 0.482579480911717 1116 72-73 0.5179401392489353 1116 74-75 0.49022918068364874 1116 76 0.8315436202925994 2118 1 0.11756821579611199 2118 2 -0.04784600939884598 2118 3 0.010361966525749722 2118 4 0.053908987975994194 2118 5 0.08036329117803831 2118 6 0.054893225212850894 2118 7 0.13129115774677302 2118 8 0.14756054700291088 2118 9 0.19370505540463512 2118 10-11 0.12125123246261893 2118 12-13 0.1273330161773245 2118 14-15 0.10682656505758814 2118 16-17 0.20365630110885036 2118 18-19 0.15222177834307615 2118 20-21 0.1290715733922454 2118 22-23 0.2032466582403032 2118 24-25 0.12521515700129981 2118 26-27 0.23984006120985413 2118 28-29 0.340472114960896 2118 30-31 0.3614771547040121 2118 32-33 0.22181529803044953 2118 34-35 0.19559570788298686 2118 36-37 0.3831522693586109 2118 38-39 0.30909314190977 2118 40-41 1.2619434299821393 2118 42-43 0.56433386922577 2118 44-45 0.26333500518003916 2118 46-47 0.7740209170206569 2118 48-49 0.37547965843541675 2118 50-51 0.25157501470624055 2118 52-53 0.3306412872057294 2118 54-55 0.19717137475083035 2118 56-57 0.2879779461095424 2118 58-59 0.09976348270414803 2118 60-61 0.2890011871790108 2118 62-63 0.2596284932395818 2118 64-65 0.3404812627681295 2118 66-67 0.4050574046744657 2118 68-69 0.140052100344235 2118 70-71 0.33071646618090256 2118 72-73 0.22683513981404957 2118 74-75 0.3516232707764644 2118 76 0.6789371578228938 1119 1 0.07640090408295919 1119 2 0.06959058221364245 1119 3 -0.005726064843209144 1119 4 -0.07564430938726474 1119 5 -0.042890158073014106 1119 6 -0.011455684563259183 1119 7 -0.019033272038484483 1119 8 0.06060128979142121 1119 9 0.17202323173428624 1119 10-11 0.11186119858972887 1119 12-13 0.1309929400427734 1119 14-15 0.12040876558761227 1119 16-17 0.09510101458746334 1119 18-19 0.22858066568555557 1119 20-21 0.1599851880039722 1119 22-23 0.11283179484399142 1119 24-25 0.07273093363091476 1119 26-27 0.20476859768516675 1119 28-29 0.2070917536077559 1119 30-31 0.2451946561180236 1119 32-33 0.2375490304664396 1119 34-35 0.230582330317894 1119 36-37 0.34287277039768327 1119 38-39 0.3018654436771371 1119 40-41 1.3913858301905861 1119 42-43 0.6046400597655079 1119 44-45 0.2634814911065888 1119 46-47 0.7365694543856804 1119 48-49 0.3696418288420844 1119 50-51 0.21617848588251576 1119 52-53 0.23873010661177574 1119 54-55 0.3751044297291273 1119 56-57 0.29375349535232687 1119 58-59 0.40214443508509845 1119 60-61 0.28697993559816837 1119 62-63 0.30265658930384376 1119 64-65 0.20068125895505062 1119 66-67 0.361036953840987 1119 68-69 0.2638247643544531 1119 70-71 0.3813885220044284 1119 72-73 0.2849674919141094 1119 74-75 0.2672642182004026 1119 76 0.2831251936404513 2119 1 0.087109249444282 2119 2 0.10095564586870154 2119 3 0.10243941669849477 2119 4 7.18191570065585E-4 2119 5 0.10256699381739764 2119 6 0.06547237727139787 2119 7 0.1375878159199786 2119 8 0.2129276498376882 2119 9 0.19341832117342506 2119 10-11 0.18722022174041086 2119 12-13 0.20077785017161176 2119 14-15 0.2111263013131861 2119 16-17 0.07510804482336653 2119 18-19 0.13992605253508827 2119 20-21 0.11454296063672587 2119 22-23 0.27021187335483887 2119 24-25 0.25424651895696826 2119 26-27 0.16682692507814778 2119 28-29 0.35442821716344497 2119 30-31 0.27609038279502585 2119 32-33 0.31511727579417936 2119 34-35 0.21713749508659674 2119 36-37 0.3844856793247047 2119 38-39 0.34160395032149893 2119 40-41 1.5532216643634271 2119 42-43 0.7115556124932567 2119 44-45 0.39255603320847854 2119 46-47 0.9545696075584189 2119 48-49 0.5599334265319271 2119 50-51 0.2600860402441114 2119 52-53 0.3648226897129021 2119 54-55 0.40754170254745503 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-0.09868222697431861 2111 8 -0.2135456061020733 2111 9 -0.4986562805717938 2111 10-11 -0.3207570759271263 2111 12-13 -0.21289870260105914 2111 14-15 -0.19790574210010448 2111 16-17 -0.3319505193525316 2111 18-19 -0.32095049438059675 2111 20-21 -0.12325193255868072 2111 22-23 -0.2770023565205122 2111 24-25 -0.4207980795587787 2111 26-27 -0.2506416200544095 2111 28-29 -0.3167902232347899 2111 30-31 -0.43737767750916845 2111 32-33 -0.45932852714586403 2111 34-35 -0.4604491984896768 2111 36-37 -0.4400243471049521 2111 38-39 -0.31692191112334456 2111 40-41 0.630193455649696 2111 42-43 -0.717141264837835 2111 44-45 -0.6218350719805699 2111 46-47 0.013599495275094853 2111 48-49 -0.5746328839160455 2111 50-51 -0.8793749217171936 2111 52-53 -0.8372876965960714 2111 54-55 -0.7735368128039539 2111 56-57 -0.9000357011313938 2111 58-59 -0.8215504591213261 2111 60-61 -0.7841920144731915 2111 62-63 -1.1650447390557304 2111 64-65 -1.0428762902057542 2111 66-67 -0.698455891118428 2111 68-69 -0.8097737551750797 2111 70-71 -0.5358778048210837 2111 72-73 -1.0847579070430484 2111 74-75 -1.6253624106773472 2111 76 -0.8010903262039903 2110 1 -0.20337198846053184 2110 2 -0.1954019460948757 2110 3 -0.03769291770283445 2110 4 -0.04635607790067553 2110 5 -0.015192957360206094 2110 6 0.07652727838194551 2110 7 -0.027109091726991608 2110 8 -0.13388484163044012 2110 9 -0.33291847308994704 2110 10-11 -0.30412102917299677 2110 12-13 -0.31789190001508416 2110 14-15 -0.10141776331225572 2110 16-17 -0.47406647116837775 2110 18-19 -0.23655969822976886 2110 20-21 -0.6862905533716059 2110 22-23 -0.4522066823693507 2110 24-25 -0.3569396726548959 2110 26-27 -0.35745860566471066 2110 28-29 -0.5156010120368464 2110 30-31 -0.34447123541058744 2110 32-33 -0.4553656484106483 2110 34-35 -0.2863720164485031 2110 36-37 -0.46798595470001914 2110 38-39 -0.40482671464280884 2110 40-41 0.5362671221938768 2110 42-43 -0.5963740125860326 2110 44-45 -0.7106768448238512 2110 46-47 -0.5063564120508417 2110 48-49 -0.8795059188624705 2110 50-51 -0.9772859816296133 2110 52-53 -0.9995333444306667 2110 54-55 -0.8438904479668636 2110 56-57 -1.049197543092923 2110 58-59 -1.196276121256247 2110 60-61 -0.9824496586929783 2110 62-63 -0.9835343663534601 2110 64-65 -0.8635804819890254 2110 66-67 -1.5327339728884937 2110 68-69 -1.5320614102194199 2110 70-71 -1.2847898876040773 2110 72-73 -1.6340637453500548 2110 74-75 -1.197320363744474 2110 76 -1.3304804768539569 2109 1 -0.1897031797191957 2109 2 -0.0750138607747175 2109 3 -0.20941962525731128 2109 4 -0.16560125823266247 2109 5 -0.17147370171370824 2109 6 -0.3007925466238106 2109 7 -0.1743638171995343 2109 8 -0.26008374059010464 2109 9 -0.260256090572625 2109 10-11 -0.2940797924911891 2109 12-13 -0.43267430848848676 2109 14-15 -0.284025093812744 2109 16-17 -0.44539232468396506 2109 18-19 -0.5100844925696677 2109 20-21 -0.5798611895049959 2109 22-23 -0.6006911819820999 2109 24-25 -0.5992292504498806 2109 26-27 -0.5982881537357869 2109 28-29 -0.743913029285082 2109 30-31 -0.5822069857298047 2109 32-33 -0.7448325020727609 2109 34-35 -0.5549536420529222 2109 36-37 -1.12718466526875 2109 38-39 -0.6631996449590929 2109 40-41 -10.344912623687687 2109 42-43 -1.7479366107063115 2109 44-45 -0.8788492434590722 2109 46-47 -0.2513274207796172 2109 48-49 -0.678551404806413 2109 50-51 -0.6930113913798905 2109 52-53 -0.3662132682369972 2109 54-55 -0.8244055925045828 2109 56-57 -0.6268753108696927 2109 58-59 -0.7087988851605402 2109 60-61 -0.8294228443836431 2109 62-63 -0.5441795006343355 2109 64-65 -1.0019368757679317 2109 66-67 -0.8884327985850149 2109 68-69 -1.4387754537167936 2109 70-71 -1.2764891738117683 2109 72-73 -0.9045868441715683 2109 74-75 -1.2622095270794134 2109 76 -0.788333238874344 2108 1 -0.09083073433512112 2108 2 -0.12259666556682447 2108 3 -0.15151029944100713 2108 4 -0.005916033896276929 2108 5 0.02122068400393573 2108 6 -0.06350572491838591 2108 7 -0.36355573639145433 2108 8 -0.25802125527179953 2108 9 -0.24244542578521333 2108 10-11 -0.5205291699870784 2108 12-13 -0.544806091434225 2108 14-15 -0.4092670482407499 2108 16-17 -0.6741214766689296 2108 18-19 -0.9017852207820312 2108 20-21 -1.0283037546917484 2108 22-23 -0.6215521169127953 2108 24-25 -0.9020551842060485 2108 26-27 -0.9487732371278526 2108 28-29 -1.485551507086349 2108 30-31 -0.6460993982268732 2108 32-33 -1.3688364954953585 2108 34-35 -0.9037262518720368 2108 36-37 -1.8369133474392854 2108 38-39 -0.97828656062741 2108 40-41 -8.211433647883133 2108 42-43 -0.9237282480095814 2108 44-45 -0.596336910830459 2108 46-47 0.12809353294365877 2108 48-49 -0.16028149642022527 2108 50-51 -0.4907128243138814 2108 52-53 -0.8032957206682809 2108 54-55 -0.8240884677688527 2108 56-57 -0.6508147048090933 2108 58-59 -0.688569850629186 2108 60-61 -1.1351064243695461 2108 62-63 -0.7896234752643565 2108 64-65 -0.5941250364444741 2108 66-67 -0.8445051500061993 2108 68-69 -0.4869513992183201 2108 70-71 -0.5440473133466526 2108 72-73 -0.7590747464501604 2108 74-75 -0.8723093626443656 2108 76 -1.5446498937956505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 8.0 16 18.0 17 22.0 18 24.0 19 35.0 20 33.0 21 44.0 22 63.0 23 108.0 24 180.0 25 356.0 26 560.0 27 990.0 28 1656.0 29 2465.0 30 3800.0 31 5527.0 32 8701.0 33 12575.0 34 18875.0 35 31020.0 36 66576.0 37 286692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.898671220837144 25.857938041782397 7.9247562617951575 24.318634475585302 2 14.907262524158071 23.095685271694574 41.766497323592134 20.23055488055522 3 17.805095735234335 28.19868779935003 32.44619364157255 21.550022823843083 4 12.995510175346162 17.593208714393104 42.2157068918922 27.19557421836854 5 15.22361688646444 30.652534809199484 33.55173063777312 20.572117666562956 6 24.81122070088502 38.02134313206716 20.75743364620545 16.41000252084237 7 22.024667918760745 34.98656686250508 25.2724667237452 17.716298494988976 8 19.50541526903747 40.54899858969089 23.566015411203896 16.379570730067744 9 20.716555121284312 19.064154302805402 25.378296682707703 34.84099389320258 10-11 22.470811597691725 26.508701448235293 31.27547810841439 19.74500884565859 12-13 20.74778177226574 25.26429101876097 31.477372600941568 22.510554608031722 14-15 20.29675538063584 28.33245141700865 26.160212023282593 25.21058117907292 16-17 20.025253844284617 32.84498636246988 27.356817288890806 19.7729425043547 18-19 19.37948670198874 31.516093648158538 30.056048999725206 19.04837065012752 20-21 19.55742183685381 29.697112840625987 29.935457351207845 20.81000797131236 22-23 19.795198590145098 29.923420896647734 30.16278737035262 20.118593142854547 24-25 19.46135730328913 31.025551349104873 29.82315495913283 19.689936388473164 26-27 19.24345205516784 31.056891551544414 29.930915292883277 19.768741100404473 28-29 18.950035087400558 31.144099071376175 30.126337352297938 19.779528488925326 30-31 19.03508512952815 30.88304426917146 30.65684976460783 19.425020836692564 32-33 19.231302049149612 30.629938069034747 30.512185206970244 19.626574674845397 34-35 19.473053103474903 30.042763479125835 30.91551998619214 19.56866343120712 36-37 19.888992094547486 30.45450106624819 30.593828705354404 19.062678133849918 38-39 19.54856482312089 30.180160743444105 30.314832772767637 19.956441660667366 40-41 19.91170238617034 30.17595933949388 29.93886389495127 19.973474379384506 42-43 20.061703862339296 30.25044909601684 29.951808761176302 19.73603828046756 44-45 19.521539576089697 30.023119076872067 30.251811713514215 20.203529633524024 46-47 19.68266909515385 30.248178066854557 30.438944516486536 19.63020832150506 48-49 19.65746067145248 30.487430989101334 30.013580754390468 19.841527585055722 50-51 19.43262878438622 30.604502542417144 30.21740562170559 19.745463051491043 52-53 18.926757038487132 30.87872931376312 30.551246908561552 19.6432667391882 54-55 18.98353276754427 30.6566226616916 30.683420805806566 19.676423764957566 56-57 18.9352733978457 30.29802715696672 31.15488645989703 19.611812985290545 58-59 18.636405960088933 30.71237642762571 31.272185116129076 19.379032496156285 60-61 19.53811808897438 29.976562979045212 31.185204699213543 19.300114232766862 62-63 19.472031140351874 29.42027438574339 31.233123414537765 19.874571059366975 64-65 19.431152615430737 29.354755194411453 31.281609887152563 19.93248230300525 66-67 19.122973958108595 29.831103561200827 30.75404981275365 20.29187266793693 68-69 19.279107213015724 29.493515076227094 30.56441887770281 20.662958833054375 70-71 19.175094077383047 29.65475814674936 30.535803910258014 20.634343865609576 72-73 18.808663522048285 29.68984554730667 30.798221329960096 20.703269600684944 74-75 19.011352874782265 29.807598409371174 30.289851451982496 20.891197263864065 76 19.080959918606315 29.15728920875073 30.69046099620965 21.0712898764333 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 2.5 8 5.0 9 7.0 10 15.5 11 36.5 12 51.0 13 82.0 14 143.5 15 225.5 16 433.5 17 590.0 18 818.5 19 1353.5 20 2054.5 21 2449.0 22 2986.5 23 4307.5 24 5835.0 25 6579.0 26 7552.5 27 9542.5 28 11477.5 29 12396.0 30 13717.5 31 15894.0 32 17577.5 33 18406.0 34 19245.5 35 21338.0 36 23386.0 37 24181.0 38 24352.5 39 24942.5 40 25599.0 41 25716.5 42 25596.0 43 25573.0 44 24928.5 45 22879.0 46 21451.0 47 20061.0 48 17197.0 49 14562.5 50 13402.0 51 12458.0 52 10239.0 53 8135.0 54 7306.0 55 6479.5 56 5002.0 57 3816.0 58 3281.0 59 2957.0 60 2294.0 61 1642.5 62 1330.0 63 1160.0 64 833.5 65 535.0 66 335.0 67 277.0 68 225.5 69 145.0 70 93.0 71 70.0 72 56.5 73 32.5 74 17.5 75 13.0 76 9.0 77 6.5 78 5.5 79 3.0 80 2.0 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 440329.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.60468154889396 #Duplication Level Percentage of deduplicated Percentage of total 1 95.603864415112 86.62157690174858 2 3.0270404781433906 5.485280771155872 3 0.6295418458701358 1.7111831540029963 4 0.19621574359010996 0.7111225985144538 5 0.14427540996771263 0.6536013787730363 6 0.0785106606935132 0.42680600461974183 7 0.07202731297810286 0.4568208228642469 8 0.04361111619760903 0.31610970360609425 9 0.030968719799287947 0.25253198958522705 >10 0.16397484996819317 2.3692574509335227 >50 0.007617531785360525 0.4947337153028333 >100 0.0023519158946106373 0.5009755088933918 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.813087486856418E-4 0.0 0.0 2 6.813087486856418E-4 0.0 0.0 3 6.813087486856418E-4 0.0 0.0 4 6.813087486856418E-4 0.0 0.0 5 6.813087486856418E-4 0.0 0.0 6 6.813087486856418E-4 0.0 0.0 7 6.813087486856418E-4 0.0 0.0 8 6.813087486856418E-4 0.0 0.0 9 6.813087486856418E-4 0.0 0.0 10 6.813087486856418E-4 0.0 0.0 11 6.813087486856418E-4 0.0 0.0 12 6.813087486856418E-4 0.0 0.0 13 6.813087486856418E-4 0.0 0.0 14 6.813087486856418E-4 0.0 0.0 15 6.813087486856418E-4 0.0 0.0 16 6.813087486856418E-4 2.271029162285473E-4 0.0 17 6.813087486856418E-4 2.271029162285473E-4 0.0 18 6.813087486856418E-4 2.271029162285473E-4 0.0 19 6.813087486856418E-4 4.542058324570946E-4 0.0 20 6.813087486856418E-4 4.542058324570946E-4 0.0 21 6.813087486856418E-4 4.542058324570946E-4 0.0 22 6.813087486856418E-4 4.542058324570946E-4 0.0 23 6.813087486856418E-4 4.542058324570946E-4 0.0 24 6.813087486856418E-4 4.542058324570946E-4 2.271029162285473E-4 25 6.813087486856418E-4 4.542058324570946E-4 2.271029162285473E-4 26 6.813087486856418E-4 4.542058324570946E-4 2.271029162285473E-4 27 9.084116649141892E-4 4.542058324570946E-4 6.813087486856418E-4 28 0.0011355145811427364 4.542058324570946E-4 0.0011355145811427364 29 0.0011355145811427364 4.542058324570946E-4 0.0020439262460569254 30 0.0011355145811427364 4.542058324570946E-4 0.0029523379109711147 31 0.0011355145811427364 4.542058324570946E-4 0.0034065437434282093 32 0.0011355145811427364 4.542058324570946E-4 0.0045420583245709455 33 0.0011355145811427364 4.542058324570946E-4 0.005904675821942229 34 0.0011355145811427364 4.542058324570946E-4 0.007948602067999156 35 0.0011355145811427364 4.542058324570946E-4 0.0124906603925701 36 0.0011355145811427364 4.542058324570946E-4 0.01998505662811216 37 0.0011355145811427364 4.542058324570946E-4 0.03406543743428209 38 0.0013626174973712837 4.542058324570946E-4 0.04950843573782331 39 0.0013626174973712837 4.542058324570946E-4 0.07721499151770608 40 0.0013626174973712837 4.542058324570946E-4 0.1046944443813603 41 0.0013626174973712837 4.542058324570946E-4 0.14012249931301368 42 0.0013626174973712837 4.542058324570946E-4 0.17986550965300946 43 0.0013626174973712837 4.542058324570946E-4 0.23073656288820404 44 0.0013626174973712837 4.542058324570946E-4 0.28683098319665523 45 0.0013626174973712837 4.542058324570946E-4 0.36427307763058986 46 0.0013626174973712837 4.542058324570946E-4 0.4571581703680657 47 0.0013626174973712837 4.542058324570946E-4 0.5625339234981116 48 0.0013626174973712837 4.542058324570946E-4 0.6726788378689571 49 0.0013626174973712837 6.813087486856418E-4 0.8078050730249428 50 0.0013626174973712837 6.813087486856418E-4 0.950652807332699 51 0.0013626174973712837 6.813087486856418E-4 1.0994052174623974 52 0.0013626174973712837 6.813087486856418E-4 1.2658716550579225 53 0.001589720413599831 6.813087486856418E-4 1.431656783904762 54 0.001589720413599831 6.813087486856418E-4 1.6003942506625728 55 0.001589720413599831 6.813087486856418E-4 1.7970653761164948 56 0.001589720413599831 6.813087486856418E-4 1.9944178103191024 57 0.001589720413599831 6.813087486856418E-4 2.213345021563422 58 0.001589720413599831 6.813087486856418E-4 2.4368142911323125 59 0.001589720413599831 6.813087486856418E-4 2.6730013240100017 60 0.0018168233298283783 6.813087486856418E-4 2.967326703442199 61 0.0018168233298283783 6.813087486856418E-4 3.2319016008484565 62 0.0018168233298283783 6.813087486856418E-4 3.5132821140556265 63 0.0018168233298283783 6.813087486856418E-4 3.8089701109851952 64 0.0018168233298283783 6.813087486856418E-4 4.107610445825735 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCTA 40 0.0024178177 35.000004 57 ACAGGCC 70 3.4860044E-5 30.0 3 GTGTAGC 75 5.5803568E-5 28.0 1 GGTTGGG 110 1.0145195E-6 25.454548 67 GTTATAG 75 0.0018312437 23.333334 3 TGGGGTC 140 3.2774005E-7 22.5 66 AGGACTG 110 2.9181752E-5 22.272728 5 GTGGGTT 115 4.1120933E-5 21.304348 64 GGGTTGG 135 6.010765E-6 20.74074 66 GGTAGGA 170 9.287942E-8 20.588236 11 GGGGTCT 155 8.7240915E-7 20.32258 67 CTTATAG 105 5.459208E-4 20.0 3 CTCCGTG 90 0.0052717985 19.444443 7 CGGTAGG 180 1.6968988E-7 19.444443 10 GTGTAAG 110 7.467107E-4 19.09091 1 AATTACG 110 7.467107E-4 19.09091 5 GATAGTG 150 1.4930549E-5 18.666666 60 CGGTAGT 95 0.007203714 18.421053 51 ACGGTAG 190 2.996585E-7 18.421053 9 GTACAGA 135 1.4099371E-4 18.148148 1 >>END_MODULE