##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve18.3410000000c8a3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1521018 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.71944644968041 34.0 34.0 34.0 34.0 34.0 2 33.76486931778585 34.0 34.0 34.0 34.0 34.0 3 33.78412089797754 34.0 34.0 34.0 34.0 34.0 4 33.80504635711083 34.0 34.0 34.0 34.0 34.0 5 33.81182865686008 34.0 34.0 34.0 34.0 34.0 6 37.58747956960404 38.0 38.0 38.0 37.0 38.0 7 37.612214977074565 38.0 38.0 38.0 37.0 38.0 8 37.509501531211335 38.0 38.0 38.0 37.0 38.0 9 37.48535323053376 38.0 38.0 38.0 37.0 38.0 10-11 37.48229080786684 38.0 38.0 38.0 37.0 38.0 12-13 37.41544051418195 38.0 38.0 38.0 37.0 38.0 14-15 37.47162393870421 38.0 38.0 38.0 37.0 38.0 16-17 37.41805849766406 38.0 38.0 38.0 37.0 38.0 18-19 37.43698233682967 38.0 38.0 38.0 37.0 38.0 20-21 37.38529327069108 38.0 38.0 38.0 37.0 38.0 22-23 37.440122667844825 38.0 38.0 38.0 37.0 38.0 24-25 37.36819912716352 38.0 38.0 38.0 37.0 38.0 26-27 37.32587089699136 38.0 38.0 38.0 37.0 38.0 28-29 37.249220259063335 38.0 38.0 38.0 37.0 38.0 30-31 37.281838544974484 38.0 38.0 38.0 37.0 38.0 32-33 37.23416652531397 38.0 38.0 38.0 37.0 38.0 34-35 37.278054237359456 38.0 38.0 38.0 37.0 38.0 36-37 37.17827994146026 38.0 38.0 38.0 36.5 38.0 38-39 37.23947809953597 38.0 38.0 38.0 37.0 38.0 40-41 36.081882988892964 38.0 37.5 38.0 32.5 38.0 42-43 36.74684158898843 38.0 37.0 38.0 35.0 38.0 44-45 36.95258701737915 38.0 38.0 38.0 36.0 38.0 46-47 36.5205717486578 38.0 37.5 38.0 35.0 38.0 48-49 36.85615554845505 38.0 37.5 38.0 35.0 38.0 50-51 36.91725344473241 38.0 38.0 38.0 36.0 38.0 52-53 36.92881576680881 38.0 38.0 38.0 36.0 38.0 54-55 36.92065215533281 38.0 38.0 38.0 36.0 38.0 56-57 36.92134642719547 38.0 38.0 38.0 36.0 38.0 58-59 36.88404772330111 38.0 38.0 38.0 36.0 38.0 60-61 36.8828179548171 38.0 38.0 38.0 36.0 38.0 62-63 36.89196873409782 38.0 38.0 38.0 36.0 38.0 64-65 36.81533256016694 38.0 38.0 38.0 35.5 38.0 66-67 36.780805684087895 38.0 38.0 38.0 35.0 38.0 68-69 36.76737454783573 38.0 38.0 38.0 35.0 38.0 70-71 36.689702554473385 38.0 37.5 38.0 34.5 38.0 72-73 36.73517473165998 38.0 37.5 38.0 35.0 38.0 74-75 36.72756042334805 38.0 38.0 38.0 35.0 38.0 76 36.01338642935192 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0552168196602878 1101 2 0.07391428290370072 1101 3 0.07805617256549624 1101 4 0.01677497901224001 1101 5 0.06598906796751436 1101 6 0.10999387720365661 1101 7 0.0837706692055491 1101 8 0.15383778084356692 1101 9 0.1293350650288474 1101 10-11 0.12649105120780746 1101 12-13 0.19232465205798377 1101 14-15 0.13158794691816667 1101 16-17 0.22012980547069105 1101 18-19 0.14931998128352575 1101 20-21 0.18864839064463723 1101 22-23 0.11377298502912936 1101 24-25 0.12034979509386545 1101 26-27 0.12434430253772177 1101 28-29 0.1380887357711984 1101 30-31 0.19755494220630254 1101 32-33 0.2685861124051101 1101 34-35 0.1523754096836214 1101 36-37 0.2406827474463924 1101 38-39 0.21358490319098422 1101 40-41 1.4558118021754183 1101 42-43 0.6478334618228558 1101 44-45 0.32086438061438827 1101 46-47 0.8047574608860941 1101 48-49 0.4824675036916304 1101 50-51 0.33126834804374994 1101 52-53 0.35040470301478166 1101 54-55 0.3325998248674509 1101 56-57 0.27240431006084975 1101 58-59 0.303976007545387 1101 60-61 0.4069476797586944 1101 62-63 0.325566033680488 1101 64-65 0.39387019314495575 1101 66-67 0.3295623485864496 1101 68-69 0.4087149332479143 1101 70-71 0.4085646638193907 1101 72-73 0.3889420693419581 1101 74-75 0.3709809054822557 1101 76 0.5152844146942499 1102 1 0.0476667236326449 1102 2 0.09676268838808255 1102 3 0.07917810569933437 1102 4 0.06425275999191626 1102 5 0.05618269587930769 1102 6 0.08559100788382779 1102 7 0.12605923488828097 1102 8 0.11229155139989899 1102 9 0.12091480091331164 1102 10-11 0.1863741224053186 1102 12-13 0.2082577553671996 1102 14-15 0.14984541541706164 1102 16-17 0.1947814856468284 1102 18-19 0.20671829346382964 1102 20-21 0.22003157627580805 1102 22-23 0.1556341266949346 1102 24-25 0.20273129914318844 1102 26-27 0.23353115140797343 1102 28-29 0.28578297977979616 1102 30-31 0.2593033535331699 1102 32-33 0.3227064272141007 1102 34-35 0.20848919847905023 1102 36-37 0.09992711515755559 1102 38-39 0.2165434856719628 1102 40-41 1.4572822802665328 1102 42-43 0.7959704773577272 1102 44-45 0.4525958421409584 1102 46-47 0.9435844685929027 1102 48-49 0.5712001310797987 1102 50-51 0.40046889490927384 1102 52-53 0.401771444023062 1102 54-55 0.4034571065385606 1102 56-57 0.4436973676493139 1102 58-59 0.438526496293548 1102 60-61 0.4388630588086855 1102 62-63 0.4104648861809892 1102 64-65 0.42969918707755994 1102 66-67 0.49070411322824725 1102 68-69 0.40437296086837904 1102 70-71 0.5324124749515704 1102 72-73 0.5661231618254448 1102 74-75 0.5836653446562536 1102 76 0.6897812579943619 1103 1 -0.013392101175035975 1103 2 -0.009666208999895787 1103 3 0.02798683271920055 1103 4 0.017900789024032804 1103 5 0.013612614606763884 1103 6 0.0671539289147276 1103 7 0.09349083773933842 1103 8 0.15236760859490772 1103 9 0.16440403338756227 1103 10-11 0.1389246461134377 1103 12-13 0.18281654214628418 1103 14-15 0.06039544475341074 1103 16-17 0.16296562011594062 1103 18-19 0.181325892664141 1103 20-21 0.1837619915480886 1103 22-23 0.1705771381252461 1103 24-25 0.1972905205348212 1103 26-27 0.1811307863334406 1103 28-29 0.23296193692699774 1103 30-31 0.1864329821567523 1103 32-33 0.24819949514404982 1103 34-35 0.11789204084507787 1103 36-37 0.08174909242733008 1103 38-39 0.12637485600530596 1103 40-41 1.334073834658291 1103 42-43 0.6035693517112364 1103 44-45 0.3546682648885735 1103 46-47 0.8444810471056599 1103 48-49 0.3832092090492907 1103 50-51 0.25854347985429627 1103 52-53 0.2389055817517729 1103 54-55 0.2473657944432972 1103 56-57 0.25817390400465 1103 58-59 0.3165092421755986 1103 60-61 0.28240115803072285 1103 62-63 0.23986452327951469 1103 64-65 0.25526293387229515 1103 66-67 0.28409675306482995 1103 68-69 0.30072854455301723 1103 70-71 0.31799990812989165 1103 72-73 0.33930843715248926 1103 74-75 0.2758664670431443 1103 76 0.3360203383542242 2116 1 0.029692707138664787 2116 2 0.016771852695185885 2116 3 0.05090396477579873 2116 4 0.034810545499411205 2116 5 0.06693551233512096 2116 6 0.04524187606273955 2116 7 0.0904396190291692 2116 8 0.13914553402924668 2116 9 0.16515898418531094 2116 10-11 0.15589109183877525 2116 12-13 0.19256260909178025 2116 14-15 0.07561601920085081 2116 16-17 0.16921035135835183 2116 18-19 0.2073694939262012 2116 20-21 0.23265475755410847 2116 22-23 0.13593141876149417 2116 24-25 0.2564763259379603 2116 26-27 0.2002930844493207 2116 28-29 0.15732554166759627 2116 30-31 0.19302755075047173 2116 32-33 0.2717177071770607 2116 34-35 0.14771490732096737 2116 36-37 0.2238198534383855 2116 38-39 0.18372342423486998 2116 40-41 1.376627595688376 2116 42-43 0.5908771286827914 2116 44-45 0.37614971315488077 2116 46-47 0.836136558001904 2116 48-49 0.45480199761143325 2116 50-51 0.31681073461078313 2116 52-53 0.3494993785455449 2116 54-55 0.3099277082897629 2116 56-57 0.24616385429965248 2116 58-59 0.3473487105032689 2116 60-61 0.4132670143074435 2116 62-63 0.3761653458986842 2116 64-65 0.4540202815980692 2116 66-67 0.4655569496564169 2116 68-69 0.3930520700482347 2116 70-71 0.42120654669651714 2116 72-73 0.3613932481837949 2116 74-75 0.343565524849339 2116 76 0.5793822880730914 1117 1 0.020213705043772734 1117 2 0.05988504817447904 1117 3 0.05149139776342082 1117 4 0.013520252929204446 1117 5 0.025460351148225868 1117 6 0.03410722997616489 1117 7 0.11783964660661894 1117 8 0.07950065188963862 1117 9 0.14486795898516647 1117 10-11 0.12460463685398793 1117 12-13 0.11291751098416114 1117 14-15 0.05367458308003137 1117 16-17 0.12145101946370573 1117 18-19 0.14699771330318612 1117 20-21 0.12452970875364855 1117 22-23 0.16058483163750026 1117 24-25 0.15378486614373088 1117 26-27 0.22106447234281745 1117 28-29 0.22105537565464317 1117 30-31 0.2260513022477113 1117 32-33 0.19750428758942462 1117 34-35 0.15391061420918817 1117 36-37 0.2873166081037084 1117 38-39 0.20520285494319523 1117 40-41 1.4020225264207937 1117 42-43 0.5551414834949782 1117 44-45 0.3149962944643434 1117 46-47 0.7726872332341941 1117 48-49 0.45580151417535575 1117 50-51 0.28890235156328004 1117 52-53 0.3020047735925644 1117 54-55 0.3400749320209968 1117 56-57 0.30372290078427255 1117 58-59 0.2848076142828688 1117 60-61 0.37381696853883994 1117 62-63 0.26258353306320714 1117 64-65 0.37611630097537585 1117 66-67 0.3267186748088804 1117 68-69 0.33049195203002313 1117 70-71 0.3279047945314346 1117 72-73 0.29054818197298715 1117 74-75 0.3041328395861598 1117 76 0.6105104218935153 2117 1 0.04565540115684996 2117 2 0.049991055241612514 2117 3 0.08352908916506863 2117 4 0.06699492901872617 2117 5 0.06173832523538891 2117 6 -0.004997599234670247 2117 7 0.11477722022263492 2117 8 0.04298710654217075 2117 9 0.133560538490471 2117 10-11 0.030022775841032967 2117 12-13 -0.021358107390398118 2117 14-15 0.01080061370429064 2117 16-17 0.12840037164273355 2117 18-19 0.1467621420428813 2117 20-21 0.1531516168808551 2117 22-23 0.16341168676117945 2117 24-25 0.17027485436516798 2117 26-27 0.1925603498457633 2117 28-29 0.22930036976536172 2117 30-31 0.21262374041025822 2117 32-33 0.2505078211583225 2117 34-35 0.18606609124098128 2117 36-37 0.30145088372208306 2117 38-39 0.09790014587370166 2117 40-41 1.365273409813014 2117 42-43 0.6604418368518665 2117 44-45 0.4323107820968559 2117 46-47 0.887057080112875 2117 48-49 0.4205836107595644 2117 50-51 0.30174098524996396 2117 52-53 0.30954305392236847 2117 54-55 0.3845979002188784 2117 56-57 0.3690939255192518 2117 58-59 0.416256377533756 2117 60-61 0.3821797482797038 2117 62-63 0.3301924847711035 2117 64-65 -0.11363634920129329 2117 66-67 0.2785443520762527 2117 68-69 0.3699501381313226 2117 70-71 0.4721921914690128 2117 72-73 0.37770954828629755 2117 74-75 0.43181246267214135 2117 76 0.7344209048145984 1116 1 0.06220262324082171 1116 2 0.053936873851746725 1116 3 0.09057631954380696 1116 4 0.05427491043845123 1116 5 0.04386228216898047 1116 6 0.12298312373684439 1116 7 0.14578967895619144 1116 8 0.1513822358575183 1116 9 0.1682318170451893 1116 10-11 0.08387403415652273 1116 12-13 0.0332658080693804 1116 14-15 0.12966788091178216 1116 16-17 0.20641510314126776 1116 18-19 0.17924401798640588 1116 20-21 0.19681607714064597 1116 22-23 0.16481972272726608 1116 24-25 0.1763785554882773 1116 26-27 0.22538314053571895 1116 28-29 0.30958952519262795 1116 30-31 0.2321612244428337 1116 32-33 0.24026793662142154 1116 34-35 0.2080355996933534 1116 36-37 0.34580049137980495 1116 38-39 0.2833822374810566 1116 40-41 1.3864395704991566 1116 42-43 0.6181381324108486 1116 44-45 0.3576201767450655 1116 46-47 0.7778959294633978 1116 48-49 0.4953351826170902 1116 50-51 0.31050730519555714 1116 52-53 0.3329288931035066 1116 54-55 0.34600361365141197 1116 56-57 0.3640295581608086 1116 58-59 0.330719125754527 1116 60-61 0.3607576941646755 1116 62-63 0.3376527943316461 1116 64-65 0.035116118490599035 1116 66-67 0.2424534248876853 1116 68-69 0.3247528057269946 1116 70-71 0.3279852537295085 1116 72-73 0.4390907921689049 1116 74-75 0.34041869369787037 1116 76 0.5933668063723516 2118 1 0.05435095711442983 2118 2 0.01204709774402346 2118 3 0.056697644329844366 2118 4 0.08309897428292601 2118 5 0.0062196894906279 2118 6 0.12073549045003773 2118 7 0.09870504180811679 2118 8 0.15850178106047252 2118 9 0.18521382495298155 2118 10-11 0.16492621541378583 2118 12-13 0.12776624579830553 2118 14-15 0.18924697753630682 2118 16-17 0.207998865934087 2118 18-19 0.1905674698924642 2118 20-21 0.17054848253275878 2118 22-23 0.18330329521275246 2118 24-25 0.1126346941678591 2118 26-27 0.23655950670203651 2118 28-29 0.29246638554055693 2118 30-31 0.307668141000903 2118 32-33 0.2114819121923972 2118 34-35 0.2561721093730043 2118 36-37 0.2671324442682632 2118 38-39 0.24733647330779007 2118 40-41 1.3511538228206703 2118 42-43 0.5600538745543346 2118 44-45 0.28284521572168586 2118 46-47 0.7820258522599204 2118 48-49 0.37807135225745014 2118 50-51 0.2955797013700021 2118 52-53 0.3453753347091464 2118 54-55 0.30584650535264757 2118 56-57 0.38670055360269373 2118 58-59 0.3332823208008051 2118 60-61 0.358399360665949 2118 62-63 0.352787630277021 2118 64-65 0.32214589067309163 2118 66-67 0.3791437641992701 2118 68-69 0.36413319326594973 2118 70-71 0.39985494061549787 2118 72-73 0.3024832023683288 2118 74-75 0.3002892604520895 2118 76 0.6255814501139341 1119 1 0.020682649124772468 1119 2 0.03746164054233958 1119 3 0.025646034314846133 1119 4 0.01818517268193176 1119 5 0.0011660714035457431 1119 6 0.0076200912706880786 1119 7 0.03435187289031916 1119 8 0.00786906301813417 1119 9 0.08640130297247595 1119 10-11 0.052734432728868796 1119 12-13 0.1030805929259202 1119 14-15 0.09655125822760624 1119 16-17 0.09642905620638942 1119 18-19 0.11648210311359009 1119 20-21 0.05890738545956964 1119 22-23 0.08782130003028499 1119 24-25 0.10439599007907674 1119 26-27 0.14763802663624404 1119 28-29 0.14427047521436265 1119 30-31 0.1499267792456962 1119 32-33 0.15738420205320836 1119 34-35 0.10731603945082213 1119 36-37 0.17788327282559635 1119 38-39 0.22946838586213403 1119 40-41 1.1976403598247245 1119 42-43 0.5392742455722299 1119 44-45 0.21718771598384734 1119 46-47 0.6261134590967998 1119 48-49 0.3523511477546535 1119 50-51 0.1677099010049119 1119 52-53 0.20146835432645815 1119 54-55 0.24052307548101481 1119 56-57 0.21114381826076567 1119 58-59 0.23147778154185517 1119 60-61 0.21157313960110713 1119 62-63 0.23475433152802339 1119 64-65 0.22780508309902103 1119 66-67 0.07114744832478692 1119 68-69 0.12742293032719232 1119 70-71 0.259752574572488 1119 72-73 0.08892514164937637 1119 74-75 0.17835106177568605 1119 76 0.3816853272126366 2119 1 -0.012348036421848008 2119 2 0.054863687530236405 2119 3 0.033712091670977884 2119 4 6.426020467173998E-4 2119 5 0.024827041643050052 2119 6 0.01605632806347046 2119 7 0.08672905120759822 2119 8 0.0837554460358163 2119 9 0.14297130542963288 2119 10-11 0.07291724194914195 2119 12-13 0.09159418999404068 2119 14-15 0.04260242328908248 2119 16-17 0.04863013831877083 2119 18-19 0.10181625228707247 2119 20-21 0.04813765312901097 2119 22-23 0.08462659528250782 2119 24-25 0.0894802079083874 2119 26-27 0.08741922730240503 2119 28-29 0.10931280896685536 2119 30-31 0.15662714749795015 2119 32-33 0.10912717707533659 2119 34-35 0.1336039989029203 2119 36-37 0.17990500661439057 2119 38-39 0.2249579782793134 2119 40-41 1.3162441248948866 2119 42-43 0.45389990663561264 2119 44-45 0.24862245439306463 2119 46-47 0.751349739572369 2119 48-49 0.33591208924703153 2119 50-51 0.14950144902947216 2119 52-53 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-0.40150274947312425 2104 68-69 -0.5152160177286973 2104 70-71 -0.2718205099439359 2104 72-73 -0.2648711478906094 2104 74-75 -0.24693515438387692 2104 76 -0.6092964274567905 2111 1 -0.26241928777503887 2111 2 -0.13294948362045744 2111 3 -0.11776971544016845 2111 4 -0.028593141376639153 2111 5 -0.0746185240133812 2111 6 -0.004878567702817804 2111 7 -0.06667283684924286 2111 8 -0.0908867564527327 2111 9 -0.24588802541614285 2111 10-11 -0.24242544975535196 2111 12-13 -0.15023283772455898 2111 14-15 -0.13482823601216865 2111 16-17 -0.19694558171645582 2111 18-19 -0.21194896306234767 2111 20-21 -0.17754931783293415 2111 22-23 -0.1757893643965005 2111 24-25 -0.2337028305769593 2111 26-27 -0.13670281430370324 2111 28-29 -0.16337200313110145 2111 30-31 -0.2511799399833734 2111 32-33 -0.26442709006159504 2111 34-35 -0.19336215488402075 2111 36-37 -0.1815898064691268 2111 38-39 -0.20240308743289148 2111 40-41 0.9434823015259539 2111 42-43 -0.4891234872119554 2111 44-45 -0.44201262139991826 2111 46-47 0.11927456471400433 2111 48-49 -0.37600741050605535 2111 50-51 -0.6081088260917653 2111 52-53 -0.5148129826151333 2111 54-55 -0.49454129671122615 2111 56-57 -0.6643857533447886 2111 58-59 -0.49945846815172956 2111 60-61 -0.7755919469420647 2111 62-63 -0.7224926729935959 2111 64-65 -0.5730345211759911 2111 66-67 -0.29663834024065494 2111 68-69 -0.5514190886716719 2111 70-71 -0.49841756240789437 2111 72-73 -0.8496049297623571 2111 74-75 -1.2020488454671465 2111 76 -0.5031367061593031 2110 1 -0.05701572869589455 2110 2 -0.03214858694854428 2110 3 -0.060648647495440855 2110 4 -0.09950084931652725 2110 5 -0.08094600991559275 2110 6 -0.009784967701520486 2110 7 -0.0678347800921415 2110 8 -0.09955698629414655 2110 9 -0.13665313270400503 2110 10-11 -0.1930856963430756 2110 12-13 -0.2613708968241113 2110 14-15 -0.15909575285533606 2110 16-17 -0.31275428581605524 2110 18-19 -0.22441985371018092 2110 20-21 -0.43067000538747635 2110 22-23 -0.2846148441161276 2110 24-25 -0.24941543532606403 2110 26-27 -0.19620526186857035 2110 28-29 -0.26023257598223637 2110 30-31 -0.237979391113619 2110 32-33 -0.314590236498411 2110 34-35 -0.15971029264419911 2110 36-37 -0.3027354645331144 2110 38-39 -0.23441994953201828 2110 40-41 0.7190576490592449 2110 42-43 -0.2260445173533867 2110 44-45 -0.4839674351111114 2110 46-47 -0.313596179081415 2110 48-49 -0.5842176731741731 2110 50-51 -0.740780586922682 2110 52-53 -0.6660884683529318 2110 54-55 -0.6787958166439196 2110 56-57 -0.796734838102708 2110 58-59 -0.8995442032690448 2110 60-61 -0.5618592495763508 2110 62-63 -0.8391929442315416 2110 64-65 -0.6831265440754137 2110 66-67 -1.1252985240684126 2110 68-69 -1.191268799187661 2110 70-71 -0.7894493921686987 2110 72-73 -1.2858165734442863 2110 74-75 -0.8137789254772514 2110 76 -0.9136629513884102 2109 1 -0.025394078145296817 2109 2 -0.05463239757143867 2109 3 -0.14384193023420977 2109 4 -0.08225893969898834 2109 5 -0.054299128727507195 2109 6 -0.04622236969942861 2109 7 -0.22483436732223083 2109 8 -0.15108986363259191 2109 9 -0.24523991557780533 2109 10-11 -0.2481621049126943 2109 12-13 -0.2835908053749705 2109 14-15 -0.23404763225023828 2109 16-17 -0.4457103335103554 2109 18-19 -0.43065009565732737 2109 20-21 -0.46979210346431444 2109 22-23 -0.44265173873307617 2109 24-25 -0.5104491871092449 2109 26-27 -0.39441186581799315 2109 28-29 -0.5393231657510782 2109 30-31 -0.4007447618714224 2109 32-33 -0.6467096100477931 2109 34-35 -0.29200573931844787 2109 36-37 -0.7650574459763106 2109 38-39 -0.43229229290245286 2109 40-41 -10.425650960607449 2109 42-43 -1.3153240031811038 2109 44-45 -0.6012160037960896 2109 46-47 -0.08931332834848149 2109 48-49 -0.36483408136239603 2109 50-51 -0.3377011603960369 2109 52-53 -0.3368363385278812 2109 54-55 -0.6280858647060867 2109 56-57 -0.4751414816682029 2109 58-59 -0.5461985524551594 2109 60-61 -0.5988146132167955 2109 62-63 -0.37846729350378894 2109 64-65 -0.5532507379899201 2109 66-67 -0.530338992128577 2109 68-69 -0.9446030234724319 2109 70-71 -0.8388635172656649 2109 72-73 -0.5329484838799843 2109 74-75 -0.7487914254541863 2109 76 -0.49557347935864016 2108 1 -0.04846103508453581 2108 2 -0.1019472097022458 2108 3 -0.08387184043697005 2108 4 -0.011333242746616179 2108 5 -0.06277782324728065 2108 6 -0.1563834256625256 2108 7 -0.23737852710275575 2108 8 -0.24487619143468464 2108 9 -0.32406231254553575 2108 10-11 -0.32338079224228977 2108 12-13 -0.46303519595417697 2108 14-15 -0.36066896215307764 2108 16-17 -0.6538683687995288 2108 18-19 -0.7280320449815747 2108 20-21 -0.7075126608063087 2108 22-23 -0.4946643218757387 2108 24-25 -0.8901545797751993 2108 26-27 -0.8060083416317809 2108 28-29 -1.453398553322593 2108 30-31 -0.7192544777728074 2108 32-33 -1.2769486017970877 2108 34-35 -0.8012189863696548 2108 36-37 -1.4145579819076985 2108 38-39 -0.7147415893279785 2108 40-41 -7.8998829394354075 2108 42-43 -0.9661373794422587 2108 44-45 -0.4675234031810902 2108 46-47 0.1921158325831982 2108 48-49 -0.23743688373216543 2108 50-51 -0.3174208551167155 2108 52-53 -0.6582637319021885 2108 54-55 -0.7094539774379314 2108 56-57 -0.6194953186670915 2108 58-59 -0.5619659638948704 2108 60-61 -0.8924750296273274 2108 62-63 -0.6411060784105445 2108 64-65 -0.506476271980496 2108 66-67 -0.513556109813905 2108 68-69 -0.40098488017067524 2108 70-71 -0.4611894828334471 2108 72-73 -0.6667162181779602 2108 74-75 -0.5708846515279404 2108 76 -1.2508036000023495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 3.0 14 5.0 15 20.0 16 31.0 17 82.0 18 126.0 19 164.0 20 182.0 21 205.0 22 319.0 23 471.0 24 728.0 25 1144.0 26 1839.0 27 2982.0 28 4499.0 29 6884.0 30 10261.0 31 15210.0 32 22735.0 33 34429.0 34 51386.0 35 84431.0 36 181572.0 37 1101306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.91526464512583 25.901205639906955 12.871313817456468 23.312215897510747 2 14.063344418014779 23.281578521753193 42.57878604986923 20.076291010362795 3 17.988610259707645 26.873777956605377 32.65720721253792 22.48040457114906 4 12.773221618679068 16.520383059240586 42.70166428010714 28.0047310419732 5 11.880464268009977 37.73854089826682 38.439781777730445 11.941213055992762 6 27.522685464603313 41.56453112323457 17.942259723422076 12.970523688740041 7 22.988353852485638 33.748121324008004 25.7399320718098 17.52359275169656 8 20.02270847550785 41.77544249969428 23.24081634799851 14.961032676799354 9 21.968379072437013 17.300386977668904 25.84525626915658 34.885977680737504 10-11 22.683459367344767 27.55444051286704 31.35242317973883 18.409676940049362 12-13 20.681609290619836 25.408706537332233 32.507471969431 21.402212202616933 14-15 19.62646069934741 28.467513204971933 26.75806597949531 25.14796011618534 16-17 18.96019639478297 34.00696112735023 28.379940276840905 18.652902201025892 18-19 18.460432420918096 32.70230858543423 30.83661731813825 18.000641675509428 20-21 18.92272149310528 30.39096184266064 31.223759350645423 19.462557313588665 22-23 19.03991274264999 30.78326489232869 31.083622942003313 19.093199423018007 24-25 19.044251941791615 31.187993830447763 30.899831560178775 18.867922667581844 26-27 18.889454299686133 31.451501560139327 30.683989275603572 18.975054864570964 28-29 18.676636305421766 31.525958272683162 31.01541204640576 18.78199337548931 30-31 18.8728864484181 31.01564215545115 31.21475222515447 18.89671917097628 32-33 18.863813577485605 31.29374537316455 30.801509252355984 19.040931796993853 34-35 18.696096956117547 31.043288113618644 31.298347554072336 18.962267376191473 36-37 18.799777517425827 31.358833360288962 31.276289958435733 18.565099163849474 38-39 18.70572866330313 31.128494205854235 31.058672546938958 19.107104583903674 40-41 18.875187538871995 30.97093525520408 31.129348896594255 19.02452830932967 42-43 19.1222589081786 30.927937736437045 31.018074736788126 18.93172861859623 44-45 18.55563182026774 30.741385046067833 31.40508527841222 19.297897855252206 46-47 18.51769670049927 30.636521066811834 31.815073851854482 19.030708380834415 48-49 18.41339155749636 30.7491758808903 31.63450399666539 19.20292856494795 50-51 18.384496435939614 30.68576440252515 31.61691709105349 19.312822070481744 52-53 18.209975161372185 30.50062523914904 31.936768664144672 19.352630935334098 54-55 18.10849707235549 30.44901506754029 31.99610392513435 19.44638393496987 56-57 17.942489832467466 30.406707875909422 31.96911542138226 19.681686870240853 58-59 17.644597236850583 30.60627816370352 32.038443989485984 19.71068060995991 60-61 17.92470569053095 30.39316431495222 31.701334237990608 19.98079575652622 62-63 17.686871555760682 29.857963548097395 31.982560364177147 20.472604531964777 64-65 17.69518835411547 29.70724212336738 31.8115564707321 20.786013051785055 66-67 17.89087966085871 29.9132225917116 31.28490261127745 20.910995136152234 68-69 17.740059617966388 30.073214123698733 31.118796753227112 21.067929505107763 70-71 17.76625917642 30.074463287087987 30.9585751121946 21.20070242429741 72-73 17.570600742397527 30.13731592920005 30.855880732509412 21.436202595893015 74-75 17.954291139223862 29.953425929213196 30.330213054677852 21.762069876885086 76 18.157510299023414 29.98380032320459 30.045140820161237 21.81354855761076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 1.0 5 2.0 6 6.5 7 12.5 8 15.0 9 23.0 10 63.0 11 160.5 12 226.0 13 360.5 14 720.0 15 1316.0 16 2321.5 17 2956.0 18 3976.5 19 6452.5 20 9943.5 21 11979.0 22 14512.5 23 20260.0 24 26794.5 25 30115.0 26 34152.0 27 42052.0 28 49534.5 29 53154.0 30 56944.0 31 63671.0 32 68978.5 33 71349.0 34 73623.0 35 78049.5 36 81961.5 37 83721.0 38 84542.0 39 85769.5 40 86156.5 41 84303.5 42 82470.0 43 80783.5 44 76505.0 45 70256.5 46 66600.0 47 62261.0 48 53576.5 49 45270.5 50 41310.0 51 37652.0 52 30134.0 53 23196.5 54 20119.0 55 17672.0 56 13368.0 57 9844.0 58 8177.0 59 7239.0 60 5360.5 61 3753.5 62 3087.0 63 2589.5 64 1762.0 65 1172.0 66 749.0 67 586.0 68 467.5 69 288.0 70 183.5 71 140.0 72 112.0 73 64.5 74 36.0 75 27.0 76 19.0 77 13.5 78 11.5 79 7.0 80 5.5 81 2.5 82 1.5 83 2.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1521018.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.8502030521573 #Duplication Level Percentage of deduplicated Percentage of total 1 96.14355340017111 89.26948455361816 2 2.961103484403351 5.498781195706033 3 0.41384862873919587 1.152777876338736 4 0.1589848895167652 0.5904711709542577 5 0.07597103379511638 0.352696295697943 6 0.029470881696783004 0.16418264098034446 7 0.04675781823160639 0.30390310419563615 8 0.03247458812568137 0.24122176812037527 9 0.027418773625202952 0.22912548286871062 >10 0.10538130657193728 1.6787820046688477 >50 0.003262761266358682 0.20881082847505963 >100 0.001772433856943837 0.30976307837592776 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.972363246194325E-4 0.0 0.0 2 1.972363246194325E-4 0.0 0.0 3 1.972363246194325E-4 0.0 0.0 4 1.972363246194325E-4 0.0 0.0 5 1.972363246194325E-4 0.0 0.0 6 1.972363246194325E-4 6.574544153981083E-5 0.0 7 1.972363246194325E-4 6.574544153981083E-5 0.0 8 1.972363246194325E-4 6.574544153981083E-5 0.0 9 1.972363246194325E-4 6.574544153981083E-5 0.0 10 3.287272076990542E-4 6.574544153981083E-5 0.0 11 3.287272076990542E-4 6.574544153981083E-5 6.574544153981083E-5 12 3.287272076990542E-4 6.574544153981083E-5 6.574544153981083E-5 13 3.94472649238865E-4 6.574544153981083E-5 6.574544153981083E-5 14 3.94472649238865E-4 1.3149088307962166E-4 6.574544153981083E-5 15 4.6021809077867584E-4 1.3149088307962166E-4 6.574544153981083E-5 16 4.6021809077867584E-4 1.3149088307962166E-4 1.3149088307962166E-4 17 5.259635323184867E-4 1.3149088307962166E-4 1.3149088307962166E-4 18 5.259635323184867E-4 1.3149088307962166E-4 1.972363246194325E-4 19 5.917089738582975E-4 1.3149088307962166E-4 3.287272076990542E-4 20 7.231998569379192E-4 1.3149088307962166E-4 3.94472649238865E-4 21 7.231998569379192E-4 1.3149088307962166E-4 7.231998569379192E-4 22 7.231998569379192E-4 1.972363246194325E-4 0.001183417947716595 23 7.8894529847773E-4 1.972363246194325E-4 0.0013806542723360277 24 7.8894529847773E-4 1.972363246194325E-4 0.0017751269215748927 25 7.8894529847773E-4 1.972363246194325E-4 0.002038108687734136 26 7.8894529847773E-4 1.972363246194325E-4 0.00236683589543319 27 7.8894529847773E-4 1.972363246194325E-4 0.007034762244759759 28 7.8894529847773E-4 2.6298176615924333E-4 0.015976142294174035 29 7.8894529847773E-4 2.6298176615924333E-4 0.023668358954331902 30 7.8894529847773E-4 2.6298176615924333E-4 0.02919097604367601 31 8.546907400175409E-4 3.287272076990542E-4 0.03326719341914428 32 8.546907400175409E-4 3.287272076990542E-4 0.040499191988523475 33 8.546907400175409E-4 3.287272076990542E-4 0.0509527171933534 34 8.546907400175409E-4 3.287272076990542E-4 0.07061060421375684 35 8.546907400175409E-4 3.287272076990542E-4 0.10703357882681204 36 8.546907400175409E-4 3.287272076990542E-4 0.16811109401729632 37 8.546907400175409E-4 3.287272076990542E-4 0.27310656415637424 38 9.204361815573517E-4 3.287272076990542E-4 0.4029538111975006 39 9.204361815573517E-4 3.287272076990542E-4 0.5569953807252774 40 9.861816230971627E-4 3.287272076990542E-4 0.7339821093504482 41 9.861816230971627E-4 3.287272076990542E-4 0.9108373470925394 42 9.861816230971627E-4 3.287272076990542E-4 1.1135305433597762 43 9.861816230971627E-4 3.287272076990542E-4 1.3421274435936985 44 0.0010519270646369733 3.287272076990542E-4 1.6083307363883925 45 0.0013806542723360277 3.287272076990542E-4 1.9325215086211998 46 0.0013806542723360277 3.287272076990542E-4 2.292806528259363 47 0.0013806542723360277 3.287272076990542E-4 2.664136782076215 48 0.0014463997138758383 3.287272076990542E-4 3.0426990344624456 49 0.0014463997138758383 3.287272076990542E-4 3.4250745224579853 50 0.00157789059695546 3.287272076990542E-4 3.827042152032389 51 0.00157789059695546 3.287272076990542E-4 4.214940257117273 52 0.00157789059695546 3.287272076990542E-4 4.628281848078063 53 0.001643636038495271 3.287272076990542E-4 5.042806856986571 54 0.001643636038495271 3.287272076990542E-4 5.494083567715832 55 0.001643636038495271 3.287272076990542E-4 5.984150088953583 56 0.0017093814800350818 3.94472649238865E-4 6.480265190812995 57 0.0017093814800350818 5.259635323184867E-4 6.99662988866667 58 0.0017093814800350818 5.259635323184867E-4 7.530877346619172 59 0.0017751269215748927 5.259635323184867E-4 8.044480735928174 60 0.0018408723631147034 5.259635323184867E-4 8.564395687624998 61 0.0018408723631147034 5.259635323184867E-4 9.065901915690675 62 0.0019723632461943253 5.259635323184867E-4 9.572733524521077 63 0.0019723632461943253 5.917089738582975E-4 10.067928190198932 64 0.002038108687734136 6.574544153981084E-4 10.606449101851522 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 340 0.0 19.558823 5 ACCGTGG 130 1.05792235E-4 18.846155 8 TACACTG 700 0.0 18.5 5 GTATAAG 360 0.0 18.472221 1 CACCGTG 210 4.274807E-8 18.333332 7 ATGCCGT 345 0.0 18.260868 14 TATGCCG 365 0.0 18.219177 13 GTCCTAC 135 1.412822E-4 18.14815 1 TGTGCGG 425 0.0 18.117647 10 ACTGTGC 430 0.0 17.906977 8 CTACACT 555 0.0 17.657658 4 GTATAGG 320 3.6379788E-12 17.5 1 TGTACAC 545 0.0 17.339449 3 CTTACAC 365 0.0 17.260273 3 CGTATGC 345 0.0 17.246376 11 GTATAGA 415 0.0 16.86747 1 GGGGACT 125 0.0017629473 16.800001 51 ACAGTGC 480 0.0 16.770834 8 ACTGTCC 355 1.8189894E-12 16.760561 8 GTAGCAC 460 0.0 16.73913 3 >>END_MODULE