FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve18.3410000000c8a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve18.3410000000c8a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521018
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3400.019.5588235
ACCGTGG1301.05792235E-418.8461558
TACACTG7000.018.55
GTATAAG3600.018.4722211
CACCGTG2104.274807E-818.3333327
ATGCCGT3450.018.26086814
TATGCCG3650.018.21917713
GTCCTAC1351.412822E-418.148151
TGTGCGG4250.018.11764710
ACTGTGC4300.017.9069778
CTACACT5550.017.6576584
GTATAGG3203.6379788E-1217.51
TGTACAC5450.017.3394493
CTTACAC3650.017.2602733
CGTATGC3450.017.24637611
GTATAGA4150.016.867471
GGGGACT1250.001762947316.80000151
ACAGTGC4800.016.7708348
ACTGTCC3551.8189894E-1216.7605618
GTAGCAC4600.016.739133
Produced by FastQC (version 0.11.2)