##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve17.3410000000c889.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1143958 Sequences flagged as poor quality 0 Sequence length 76 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.77343748634128 34.0 34.0 34.0 34.0 34.0 2 33.80258191297233 34.0 34.0 34.0 34.0 34.0 3 33.825344112283844 34.0 34.0 34.0 34.0 34.0 4 33.8388795742501 34.0 34.0 34.0 34.0 34.0 5 33.84531337688971 34.0 34.0 34.0 34.0 34.0 6 37.65903730731373 38.0 38.0 38.0 37.0 38.0 7 37.67746980221302 38.0 38.0 38.0 38.0 38.0 8 37.59920119444945 38.0 38.0 38.0 37.0 38.0 9 37.576780790903165 38.0 38.0 38.0 37.0 38.0 10-11 37.577347245266 38.0 38.0 38.0 37.0 38.0 12-13 37.518913718860304 38.0 38.0 38.0 37.0 38.0 14-15 37.56256130032746 38.0 38.0 38.0 37.0 38.0 16-17 37.515386054383114 38.0 38.0 38.0 37.0 38.0 18-19 37.535465899971854 38.0 38.0 38.0 37.0 38.0 20-21 37.493210415067686 38.0 38.0 38.0 37.0 38.0 22-23 37.53732567104736 38.0 38.0 38.0 37.0 38.0 24-25 37.480067887107744 38.0 38.0 38.0 37.0 38.0 26-27 37.445484886682905 38.0 38.0 38.0 37.0 38.0 28-29 37.38066213969394 38.0 38.0 38.0 37.0 38.0 30-31 37.4063296030099 38.0 38.0 38.0 37.0 38.0 32-33 37.36034801976996 38.0 38.0 38.0 37.0 38.0 34-35 37.39839661945631 38.0 38.0 38.0 37.0 38.0 36-37 37.31030335029783 38.0 38.0 38.0 37.0 38.0 38-39 37.36328169390835 38.0 38.0 38.0 37.0 38.0 40-41 36.17094945793465 38.0 37.5 38.0 33.5 38.0 42-43 36.89513557315915 38.0 37.5 38.0 35.0 38.0 44-45 37.10007010746898 38.0 38.0 38.0 36.0 38.0 46-47 36.68725162986753 38.0 38.0 38.0 35.0 38.0 48-49 37.00402593451857 38.0 38.0 38.0 35.5 38.0 50-51 37.06376195629559 38.0 38.0 38.0 36.0 38.0 52-53 37.0754844146376 38.0 38.0 38.0 36.0 38.0 54-55 37.06745439954963 38.0 38.0 38.0 36.0 38.0 56-57 37.068013423569745 38.0 38.0 38.0 36.0 38.0 58-59 37.04256100311375 38.0 38.0 38.0 36.0 38.0 60-61 37.03207591537452 38.0 38.0 38.0 36.0 38.0 62-63 37.04286870671825 38.0 38.0 38.0 36.0 38.0 64-65 36.96750754835405 38.0 38.0 38.0 36.0 38.0 66-67 36.93831067224496 38.0 38.0 38.0 36.0 38.0 68-69 36.924759912514276 38.0 38.0 38.0 36.0 38.0 70-71 36.85576786910009 38.0 38.0 38.0 35.5 38.0 72-73 36.893842256446476 38.0 38.0 38.0 36.0 38.0 74-75 36.89011572103172 38.0 38.0 38.0 36.0 38.0 76 36.22026595381998 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03319050355884201 1101 2 0.048942641073622895 1101 3 0.05707688344101314 1101 4 0.016331530227120084 1101 5 -5.220137810937331E-4 1101 6 0.06558999520991904 1101 7 -0.029147346264203122 1101 8 0.09004660571330447 1101 9 0.0730868276147163 1101 10-11 0.04156446654880597 1101 12-13 0.053676584958260776 1101 14-15 0.02543748319408934 1101 16-17 0.10088702875147959 1101 18-19 0.0663328493704256 1101 20-21 0.10245091478100932 1101 22-23 0.06656310173893587 1101 24-25 0.04019910632042212 1101 26-27 0.002606220681464322 1101 28-29 0.10498253755761056 1101 30-31 0.12883293166145648 1101 32-33 0.21081224453435965 1101 34-35 0.0812946167577735 1101 36-37 0.13690531965869468 1101 38-39 0.06342375845208892 1101 40-41 1.2688717120872894 1101 42-43 0.4964559018842678 1101 44-45 0.14909737387791466 1101 46-47 0.6814920539498956 1101 48-49 0.296256316847888 1101 50-51 0.1950284466086245 1101 52-53 0.23820767305272028 1101 54-55 0.18963841712131568 1101 56-57 0.16005468242416754 1101 58-59 0.20551769110328166 1101 60-61 0.279043510431336 1101 62-63 0.18428304681876284 1101 64-65 0.14685795158263915 1101 66-67 0.2035019039677266 1101 68-69 0.20485372568444404 1101 70-71 0.30852047176036734 1101 72-73 0.20971659393794795 1101 74-75 0.2616124214843367 1101 76 0.18645234954924916 1102 1 0.025571554285399145 1102 2 0.04191752266206805 1102 3 0.04232060509126967 1102 4 0.04520393982435422 1102 5 -0.003695389008385064 1102 6 -0.047405993480047925 1102 7 0.025592227692314395 1102 8 -0.009096307283918748 1102 9 0.004285947407225876 1102 10-11 0.059529051480765816 1102 12-13 0.04004474118004708 1102 14-15 0.04644005171907395 1102 16-17 0.08749495295806042 1102 18-19 0.07764862422411056 1102 20-21 0.10098369344549951 1102 22-23 0.05411333710003419 1102 24-25 0.09310883075014686 1102 26-27 0.10844431003973654 1102 28-29 0.11643453947408489 1102 30-31 0.13082955360624027 1102 32-33 0.09950115852323194 1102 34-35 0.08465862633012478 1102 36-37 -0.017629375896532906 1102 38-39 0.05085609396352453 1102 40-41 1.2845692050916213 1102 42-43 0.5808532926739218 1102 44-45 0.2946919922144886 1102 46-47 0.7341190380404115 1102 48-49 0.3371646406817064 1102 50-51 0.214840372851981 1102 52-53 0.24744688589525765 1102 54-55 0.2071989679360513 1102 56-57 0.2028066403553339 1102 58-59 0.2317859133656981 1102 60-61 0.32839222080122 1102 62-63 0.24139330583920326 1102 64-65 0.1734034838988947 1102 66-67 0.32291069417951945 1102 68-69 0.21847778953457464 1102 70-71 0.2921120813011271 1102 72-73 0.31175478889438324 1102 74-75 0.24875533322842358 1102 76 0.35044801200438513 1103 1 0.014942222793713711 1103 2 0.0014641324109874176 1103 3 -0.013965536721009641 1103 4 -0.005677976695722009 1103 5 0.02907568970142904 1103 6 0.0518516319054001 1103 7 0.025896250552833067 1103 8 0.07507235909415755 1103 9 0.08557306926576302 1103 10-11 0.018661018254277906 1103 12-13 0.16429865490094642 1103 14-15 0.0744574890357299 1103 16-17 0.09908441700203952 1103 18-19 0.09132047804624221 1103 20-21 0.07780122050937166 1103 22-23 0.09525255121135956 1103 24-25 0.10544834296236871 1103 26-27 0.06022989176600646 1103 28-29 0.06842238606640194 1103 30-31 0.0999246289363569 1103 32-33 0.19265424258284725 1103 34-35 0.143544288712107 1103 36-37 0.05464267576983417 1103 38-39 0.06128295206867307 1103 40-41 1.2390404263460297 1103 42-43 0.48051240339245993 1103 44-45 0.25542278977196275 1103 46-47 0.6528903019164076 1103 48-49 0.36927742023468113 1103 50-51 0.10790450207462499 1103 52-53 0.18515556276607725 1103 54-55 0.15311448961675467 1103 56-57 0.17079322081129078 1103 58-59 0.20464040443810205 1103 60-61 0.23646252640048715 1103 62-63 0.1671699195166454 1103 64-65 0.15714847836476054 1103 66-67 0.2106869525380617 1103 68-69 0.2112606871852165 1103 70-71 0.17368318720200904 1103 72-73 0.21890353823025066 1103 74-75 0.20012116322750728 1103 76 0.24864622823678673 2116 1 0.005045633899456448 2116 2 -0.01668507072408687 2116 3 0.01927347742309138 2116 4 -0.009590523036791865 2116 5 0.055447914274282084 2116 6 -0.08802914058009037 2116 7 0.03506938985812269 2116 8 0.07385212819473708 2116 9 0.08686885625802887 2116 10-11 0.04473259254103823 2116 12-13 0.12260617323589429 2116 14-15 0.010203013596495225 2116 16-17 0.046023261900494106 2116 18-19 0.05817590901819614 2116 20-21 0.09653189074229118 2116 22-23 0.0766629190184176 2116 24-25 0.11343619606116562 2116 26-27 0.1226442615274479 2116 28-29 0.013689552410696137 2116 30-31 0.0812638480893142 2116 32-33 0.12784694839764654 2116 34-35 0.007729378472745907 2116 36-37 0.05337459267828848 2116 38-39 0.062188051097528785 2116 40-41 1.253957402792679 2116 42-43 0.41370318756396074 2116 44-45 0.2608788841264129 2116 46-47 0.6502788944575073 2116 48-49 0.2959121392667612 2116 50-51 0.06800773688281225 2116 52-53 0.18249095122514092 2116 54-55 0.14357269954180651 2116 56-57 0.10990665821055501 2116 58-59 0.09747951277546463 2116 60-61 0.15727627811310185 2116 62-63 0.12696683733176428 2116 64-65 0.1919080588773383 2116 66-67 0.2407935369996963 2116 68-69 0.13295529701355235 2116 70-71 0.19729855883723957 2116 72-73 0.1618684474621901 2116 74-75 0.16826262186307162 2116 76 0.23605291646212123 1117 1 -0.019883123183078055 1117 2 -0.010701041498620611 1117 3 -0.027069706308466834 1117 4 0.006726443635223234 1117 5 -0.003918190801087462 1117 6 0.0036307609326300394 1117 7 0.055948868222991166 1117 8 0.054610276446823036 1117 9 0.07574101322295945 1117 10-11 0.08604962185336262 1117 12-13 0.09457734699845588 1117 14-15 0.08064987707067672 1117 16-17 0.08084689183153415 1117 18-19 0.11169809037109246 1117 20-21 0.12123023586870829 1117 22-23 0.07967330812334694 1117 24-25 0.045681195693681786 1117 26-27 0.11984298667177029 1117 28-29 0.16956872175001791 1117 30-31 0.12026047135877604 1117 32-33 0.14446531938198603 1117 34-35 0.07501290607457634 1117 36-37 0.15738298815080753 1117 38-39 0.07256702917508306 1117 40-41 1.257468914412037 1117 42-43 0.4521267563580622 1117 44-45 0.24276320082915248 1117 46-47 0.6618528409309477 1117 48-49 0.3365860613522358 1117 50-51 0.14410298189831394 1117 52-53 0.17163162633739404 1117 54-55 0.17939199726871635 1117 56-57 0.1188442826559779 1117 58-59 0.15528368161366757 1117 60-61 0.20362269826090085 1117 62-63 0.09366192182102395 1117 64-65 0.2557640128809666 1117 66-67 0.19292130319598044 1117 68-69 0.2161983238937637 1117 70-71 0.23757304451850558 1117 72-73 0.13302999516238145 1117 74-75 0.16455254044736733 1117 76 0.4649278880651835 2117 1 0.010395866359871775 2117 2 -0.001584250335341153 2117 3 0.035970345306957086 2117 4 0.06371541317552953 2117 5 0.059746187086581415 2117 6 0.06774856829542131 2117 7 0.053797321367014206 2117 8 0.07048594203664038 2117 9 0.12579708156165026 2117 10-11 -0.034065369363915465 2117 12-13 -0.05494034426302363 2117 14-15 -0.0648280559586425 2117 16-17 0.09819406439724077 2117 18-19 0.10772729598686226 2117 20-21 0.12624122626468193 2117 22-23 0.10782026100633146 2117 24-25 0.08295954630379754 2117 26-27 0.10730898989439908 2117 28-29 0.12761887902097158 2117 30-31 0.06419348899557775 2117 32-33 0.09321307779609356 2117 34-35 0.09529818895990161 2117 36-37 0.27191576799275197 2117 38-39 0.0758985777254253 2117 40-41 1.2141770414624347 2117 42-43 0.5242701402314935 2117 44-45 0.27024941536285496 2117 46-47 0.6977328217170538 2117 48-49 0.33703291787362843 2117 50-51 0.23139441581633946 2117 52-53 0.21339022741251767 2117 54-55 0.2426531296613632 2117 56-57 0.1826537871888121 2117 58-59 0.3146973198875287 2117 60-61 0.2784893716326735 2117 62-63 0.2094914515355697 2117 64-65 -0.1699402217288295 2117 66-67 0.06907610856768542 2117 68-69 0.3053344624942014 2117 70-71 0.3623656834780462 2117 72-73 0.2797587717207577 2117 74-75 0.27687952048186304 2117 76 0.4742022214529058 1116 1 0.04729622453519511 1116 2 0.017373804920239877 1116 3 0.04125116750041258 1116 4 -0.003959348208610436 1116 5 0.0629885875990226 1116 6 0.07459032892162298 1116 7 0.09309599531344759 1116 8 0.08881266298855195 1116 9 0.061303725244279406 1116 10-11 0.06982957761405828 1116 12-13 -0.021223696824243632 1116 14-15 0.12301611765577292 1116 16-17 0.0965930911530748 1116 18-19 0.10606103036700176 1116 20-21 0.10385275360741275 1116 22-23 0.06488544103302729 1116 24-25 0.12842861176134335 1116 26-27 0.14221578214600328 1116 28-29 0.21745888230760357 1116 30-31 0.14421857049052278 1116 32-33 0.1625014936081257 1116 34-35 0.20617795289284402 1116 36-37 0.25979507163017246 1116 38-39 0.18310897302621498 1116 40-41 1.2616709041887262 1116 42-43 0.47060557158457783 1116 44-45 0.2012397759863731 1116 46-47 0.6910243707246977 1116 48-49 0.37403210543944 1116 50-51 0.21314640204604984 1116 52-53 0.22316427596741306 1116 54-55 0.2908574530856214 1116 56-57 0.24215369160831557 1116 58-59 0.23412275536567506 1116 60-61 0.12900462594384976 1116 62-63 0.2069895902312453 1116 64-65 -0.0545951090779937 1116 66-67 0.16742046998938775 1116 68-69 0.22277387787696057 1116 70-71 0.2928724822693738 1116 72-73 0.31967054421907193 1116 74-75 0.24903373709073406 1116 76 0.44067128523359855 2118 1 0.026747789129522914 2118 2 -0.01025543338460011 2118 3 0.03243967174492468 2118 4 0.04510052999959413 2118 5 0.04375518504073739 2118 6 0.09158708969010831 2118 7 0.1102693004052071 2118 8 0.08670137077098161 2118 9 0.09852386060526896 2118 10-11 0.03595695842854241 2118 12-13 0.02486078382310808 2118 14-15 0.11674284731439855 2118 16-17 0.09410368174399508 2118 18-19 0.09578448563001984 2118 20-21 0.05055105414852079 2118 22-23 0.09021665555868452 2118 24-25 0.02660401014959035 2118 26-27 0.09583095895923321 2118 28-29 0.12001479318115571 2118 30-31 0.13425033192091718 2118 32-33 0.04992170219312442 2118 34-35 0.10147263815372298 2118 36-37 0.17114499964724672 2118 38-39 0.15693682007491816 2118 40-41 1.1907555213554062 2118 42-43 0.4360885707902824 2118 44-45 0.19235810722365443 2118 46-47 0.6523744021655133 2118 48-49 0.25023425127518806 2118 50-51 0.1259977842008979 2118 52-53 0.16593045764568615 2118 54-55 0.13805896183113475 2118 56-57 0.11550232476017896 2118 58-59 0.050013088969279806 2118 60-61 0.23852653936577184 2118 62-63 0.1555813725946038 2118 64-65 0.13752131295705539 2118 66-67 0.18646719269792555 2118 68-69 0.12210824268971976 2118 70-71 0.16301327300592305 2118 72-73 0.12438511538287145 2118 74-75 0.1175722355435127 2118 76 0.3246647033468193 1119 1 0.007759898521477737 1119 2 0.03850232490560046 1119 3 0.0236758046265777 1119 4 0.009466605710358067 1119 5 -0.017318242759770897 1119 6 -0.001124942347772162 1119 7 0.018824850207579402 1119 8 0.007387965397406049 1119 9 0.09501546855030085 1119 10-11 0.041747060557895566 1119 12-13 0.11655110389327206 1119 14-15 0.0974360737850759 1119 16-17 0.09251737388729708 1119 18-19 0.09736409888053288 1119 20-21 0.02379166718615977 1119 22-23 0.06455262123209593 1119 24-25 0.08452705042130759 1119 26-27 0.12173547609245361 1119 28-29 0.13332848202946934 1119 30-31 0.16955365772804498 1119 32-33 0.16888532485373986 1119 34-35 0.06596340090472097 1119 36-37 0.13045398030454436 1119 38-39 0.16198627909320606 1119 40-41 1.2204537358307235 1119 42-43 0.476240099017204 1119 44-45 0.17275326681417624 1119 46-47 0.5977992403051005 1119 48-49 0.2320125269642972 1119 50-51 0.1528477575532321 1119 52-53 0.11904143591070948 1119 54-55 0.12211298521100389 1119 56-57 0.09493858661760868 1119 58-59 0.16017377520826415 1119 60-61 0.10940610078538526 1119 62-63 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2104 48-49 -0.0899541763729843 2104 50-51 -0.29115763911460135 2104 52-53 -0.2565420477633964 2104 54-55 -0.4094892815564748 2104 56-57 -0.14501840834233093 2104 58-59 -0.24475230459220398 2104 60-61 -0.2539778090062015 2104 62-63 -0.13721900060232173 2104 64-65 -0.18518379111704775 2104 66-67 -0.383094907032671 2104 68-69 -0.39110350218992096 2104 70-71 -0.18235959380239564 2104 72-73 -0.2472439628055625 2104 74-75 -0.22438146335974096 2104 76 -0.4058085359007677 2111 1 -0.15340957868702532 2111 2 -0.08641110158067278 2111 3 -0.10209107844200105 2111 4 -0.019040263240370336 2111 5 -0.07560252007839097 2111 6 -0.03230781565381591 2111 7 -0.02613594766456373 2111 8 -0.12430399952727811 2111 9 -0.170445449557441 2111 10-11 -0.13136193941782892 2111 12-13 -0.038507588708363016 2111 14-15 -0.027334832769184914 2111 16-17 -0.08737504268865592 2111 18-19 -0.04801465767823743 2111 20-21 0.022369904752864045 2111 22-23 -0.08179744386364973 2111 24-25 -0.10506764196125573 2111 26-27 -0.056378684202393003 2111 28-29 -0.041106934489612 2111 30-31 -0.03432312833052009 2111 32-33 -0.1087503764612947 2111 34-35 -0.04923006643836203 2111 36-37 -0.07563377716122943 2111 38-39 -0.07058485254388103 2111 40-41 1.107373953826901 2111 42-43 -0.15297154236117905 2111 44-45 -0.18120460205440025 2111 46-47 0.3189317476991178 2111 48-49 -0.0774974162261799 2111 50-51 -0.3378848436788857 2111 52-53 -0.2883191670123537 2111 54-55 -0.18804370245504742 2111 56-57 -0.4154560001305825 2111 58-59 -0.29802281715180357 2111 60-61 -0.3435749605274552 2111 62-63 -0.4547834723505275 2111 64-65 -0.3219104610982768 2111 66-67 -0.1808129830751568 2111 68-69 -0.29420407240419166 2111 70-71 -0.16317666613652904 2111 72-73 -0.5217001048550642 2111 74-75 -0.7584386481444483 2111 76 -0.16551873223499314 2110 1 0.07085377968296314 2110 2 -0.05065990363748085 2110 3 -0.01750392638199827 2110 4 -0.015339574611211049 2110 5 -0.004147087540715688 2110 6 0.07958306012312732 2110 7 0.04555778899403862 2110 8 -0.010293728270930558 2110 9 -0.07993638196939656 2110 10-11 -0.06124356736743408 2110 12-13 -0.05431401043481543 2110 14-15 -0.012328822274277229 2110 16-17 -0.12165555546797435 2110 18-19 -0.05423265154580292 2110 20-21 -0.18273734779474893 2110 22-23 -0.08284545888913186 2110 24-25 -0.10336307002113898 2110 26-27 0.022266848647369386 2110 28-29 -0.06654938677618816 2110 30-31 -0.026479377251611425 2110 32-33 -0.0768241921508448 2110 34-35 0.0068674211038484145 2110 36-37 -0.00861004567440915 2110 38-39 -0.006174271050433333 2110 40-41 0.9454163122100283 2110 42-43 0.0594995981379256 2110 44-45 -0.1932083874863011 2110 46-47 0.08004674594438654 2110 48-49 -0.10018704905532871 2110 50-51 -0.30521474365043844 2110 52-53 -0.37016268304203237 2110 54-55 -0.3130586836470357 2110 56-57 -0.3577356034018777 2110 58-59 -0.3616071226981461 2110 60-61 -0.2191009296899722 2110 62-63 -0.3863055044964767 2110 64-65 -0.31874549175756073 2110 66-67 -0.6941040757437307 2110 68-69 -0.7152793168722553 2110 70-71 -0.4244762047681192 2110 72-73 -0.5803171063080228 2110 74-75 -0.39798442654044663 2110 76 -0.528431148030819 2109 1 0.014410617873814147 2109 2 0.008866526116570128 2109 3 -0.019220255406580122 2109 4 0.010877940791047536 2109 5 -0.03178809190703902 2109 6 0.058312148339318526 2109 7 -0.01560812273345391 2109 8 0.0037327944035254745 2109 9 -0.07804500592146013 2109 10-11 -0.16732276746354557 2109 12-13 -0.21814259622382082 2109 14-15 -0.12316215638976757 2109 16-17 -0.2540604434566518 2109 18-19 -0.21935743003857766 2109 20-21 -0.2520669388135701 2109 22-23 -0.2582318411693052 2109 24-25 -0.24188052497820678 2109 26-27 -0.1502781285824426 2109 28-29 -0.29056868934754476 2109 30-31 -0.2501431888841239 2109 32-33 -0.35355364970217096 2109 34-35 -0.1816325291508818 2109 36-37 -0.4125065314059597 2109 38-39 -0.16710202731364632 2109 40-41 -10.454907137614832 2109 42-43 -0.8911130899085649 2109 44-45 -0.319787682380003 2109 46-47 0.1994307517148357 2109 48-49 -0.009864603521698712 2109 50-51 -0.14131646755858185 2109 52-53 -0.07171577954408548 2109 54-55 -0.3436729649499526 2109 56-57 -0.1982918164918317 2109 58-59 -0.24307787905145517 2109 60-61 -0.31756024603983235 2109 62-63 -0.04935635119845472 2109 64-65 -0.1835283536684571 2109 66-67 -0.1761879190442599 2109 68-69 -0.48723164495073235 2109 70-71 -0.33148141769664363 2109 72-73 -0.1439655297584963 2109 74-75 -0.3652427724821692 2109 76 -0.23945432369355757 2108 1 0.05630236376836706 2108 2 -0.067960088280266 2108 3 -0.07080069743646078 2108 4 -0.031835665827053106 2108 5 -0.03438486311335964 2108 6 -0.00249396969994109 2108 7 -0.08143415063813109 2108 8 -0.10840296309739728 2108 9 -0.11380559364834397 2108 10-11 -0.2104665969486632 2108 12-13 -0.23847689675474015 2108 14-15 -0.16718572411417654 2108 16-17 -0.43072577250961785 2108 18-19 -0.45707967969902796 2108 20-21 -0.5502615461725071 2108 22-23 -0.25097014219412017 2108 24-25 -0.5525593124828347 2108 26-27 -0.47452919352873124 2108 28-29 -0.9517247734320051 2108 30-31 -0.41693847225244696 2108 32-33 -0.9109430650620922 2108 34-35 -0.4467617117660154 2108 36-37 -1.1027364208984949 2108 38-39 -0.5116777465382469 2108 40-41 -7.264160208981668 2108 42-43 -0.5495605062562632 2108 44-45 -0.23549100995130345 2108 46-47 0.3682150923741787 2108 48-49 -0.012862658837164531 2108 50-51 -0.08370325913778487 2108 52-53 -0.29056031281979955 2108 54-55 -0.31225704940482046 2108 56-57 -0.30089514714993726 2108 58-59 -0.2480206188958789 2108 60-61 -0.525031847472107 2108 62-63 -0.3664441089463253 2108 64-65 -0.19467170469944506 2108 66-67 -0.2930021090677215 2108 68-69 -0.1785187637308283 2108 70-71 -0.16458002554946205 2108 72-73 -0.32223406599754156 2108 74-75 -0.30224116734573414 2108 76 -0.7849962115809461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 12.0 18 29.0 19 50.0 20 104.0 21 174.0 22 278.0 23 468.0 24 645.0 25 918.0 26 1301.0 27 2117.0 28 3138.0 29 4500.0 30 6672.0 31 9953.0 32 14147.0 33 21352.0 34 31731.0 35 52128.0 36 114495.0 37 879745.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.648920677157726 25.33965407820917 11.266934625222255 23.74449061941085 2 15.77837647885674 23.126286104909447 39.381428339152315 21.7139090770815 3 18.35049888195196 26.213374966563457 31.22501000910873 24.211116142375857 4 13.550759730689412 16.78278398332806 40.798962898987554 28.867493386994976 5 13.067787453735189 36.00289521118783 36.95293009009072 13.976387244986267 6 27.419712961489846 39.27373207757627 18.873332762216794 14.433222198717086 7 24.20936782644118 33.18146295580782 24.2955598020207 18.313609415730298 8 21.160217420569634 39.01428199287037 22.92418078286091 16.901319803699085 9 22.07747137569736 17.69348175370075 24.87302855524416 35.35601831535773 10-11 23.121871607174388 26.57125523839162 30.663844301976123 19.64302885245787 12-13 22.075417104474116 25.16075765019345 30.23734262971193 22.526482615620502 14-15 20.938137588967425 27.9154479447672 25.294634942891257 25.85177952337411 16-17 20.32386678531904 32.5552161880069 27.321370190164323 19.799546836509734 18-19 19.601069270025647 31.503910108587903 29.752534621026292 19.142486000360154 20-21 19.970313595429204 28.95399131786307 29.89113236674773 21.18456271996 22-23 19.82424179908703 29.89043304037386 30.101148818400674 20.184176342138436 24-25 20.347949837319202 30.43057524839199 29.398282104762586 19.823192809526226 26-27 19.875598579668136 30.169988758328543 29.542911540458654 20.411501121544674 28-29 19.566802277706 30.505927665176518 30.06727519716633 19.85999485995115 30-31 19.84565866928681 30.042929897775966 30.16194650502903 19.949464927908192 32-33 19.751074777220843 30.28301738350534 29.72648471359963 20.239423125674193 34-35 19.49608289814836 30.06504609434962 30.440278401829442 19.998592605672584 36-37 19.80479178431376 30.552301745343797 30.179866743359458 19.463039726982984 38-39 19.6923750697141 29.786495658057376 29.941833528853333 20.579295743375194 40-41 19.58441656074786 29.905118894225136 30.517248010853542 19.99321653417346 42-43 20.345545902909024 30.232097681907906 29.718617291893583 19.70373912328949 44-45 19.451631965509222 29.696020308437898 30.3266378660755 20.525709859977376 46-47 19.35018593339965 29.733259437846492 30.98623376033036 19.93032086842349 48-49 19.347038964717235 29.715208075820964 30.63866855251679 20.299084406945013 50-51 19.32684591567173 29.772509130579973 30.493864285227257 20.406780668521048 52-53 19.185931651336848 29.770848230442027 30.867086029382197 20.176134088838925 54-55 19.22129134111567 29.940172628715388 30.769311460735448 20.069224569433494 56-57 18.927574264090115 29.42463796747783 30.911493254122966 20.73629451430909 58-59 18.339834154750438 29.611052154012647 31.61798772332551 20.43112596791141 60-61 18.986754758478895 29.66197185561008 30.841079829853896 20.51019355605713 62-63 18.637616066324114 28.813732672003695 31.065388764272818 21.48326249739938 64-65 18.656454170520245 29.03944025917035 30.964642058537112 21.339463511772287 66-67 18.977051605041446 29.237830409857708 30.30771234608264 21.477405639018215 68-69 18.5722727582656 29.154960234554068 30.331795398082797 21.940971609097538 70-71 18.609118516588897 28.983057070277056 30.610826621257075 21.796997791876972 72-73 18.479306058439207 29.182321378931743 30.40308298031921 21.93528958230984 74-75 18.905414359618096 28.734315420671035 29.687803223544922 22.672466996165944 76 18.9499090001556 28.53811066490203 29.758435187305825 22.75354514763654 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 1.5 2 0.5 3 0.5 4 0.0 5 1.0 6 2.0 7 6.0 8 10.0 9 16.0 10 39.5 11 93.0 12 129.0 13 215.0 14 411.0 15 724.0 16 1290.0 17 1653.0 18 2181.0 19 3563.0 20 5569.5 21 6722.0 22 8095.0 23 11266.0 24 15144.5 25 17225.0 26 19464.0 27 24132.0 28 28932.5 29 31304.0 30 34000.0 31 38885.5 32 42863.5 33 44652.0 34 46576.0 35 50834.5 36 55102.0 37 57035.0 38 58506.5 39 61387.5 40 63091.5 41 63239.5 42 63093.0 43 63068.0 44 62274.0 45 58627.0 46 55749.0 47 52976.5 48 47390.5 49 42068.5 50 39560.0 51 37743.0 52 31935.5 53 25279.5 54 22614.0 55 20365.5 56 16110.5 57 12526.5 58 10949.0 59 9850.0 60 7600.5 61 5569.5 62 4689.0 63 4118.5 64 3053.0 65 2216.5 66 1650.5 67 1426.0 68 1239.5 69 920.0 70 659.0 71 531.0 72 448.5 73 287.5 74 166.5 75 124.0 76 92.0 77 47.5 78 22.0 79 9.0 80 8.0 81 4.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1143958.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.7420966576018 #Duplication Level Percentage of deduplicated Percentage of total 1 96.31101739181818 87.39463649360331 2 2.535542605645404 4.601609044018855 3 0.38511542830747947 1.0483854425943306 4 0.1932381222141077 0.7013932945554411 5 0.11912236189412853 0.5404706438539416 6 0.09035172690269233 0.49192230814712085 7 0.07173878272723226 0.45568092894332424 8 0.048993922767360025 0.3556649020312688 9 0.04682477784937631 0.38240806658209525 >10 0.19072119695716575 2.972490839104951 >50 0.004208297740343098 0.2484382213801065 >100 0.0027376754892953423 0.3986290271541265 >500 1.9487561916786136E-4 0.11526407012284155 >1k 1.9283406809187596E-4 0.29300671790828703 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC 1777 0.1553378707959558 No Hit GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG 1572 0.13741763246552757 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.3707898366898083E-4 0.0 0.0 2 4.3707898366898083E-4 0.0 8.741579673379617E-5 3 5.24494780402777E-4 8.741579673379617E-5 8.741579673379617E-5 4 5.24494780402777E-4 8.741579673379617E-5 8.741579673379617E-5 5 5.24494780402777E-4 8.741579673379617E-5 8.741579673379617E-5 6 6.119105771365732E-4 8.741579673379617E-5 8.741579673379617E-5 7 6.119105771365732E-4 8.741579673379617E-5 8.741579673379617E-5 8 6.119105771365732E-4 8.741579673379617E-5 1.7483159346759234E-4 9 6.119105771365732E-4 8.741579673379617E-5 1.7483159346759234E-4 10 6.119105771365732E-4 8.741579673379617E-5 1.7483159346759234E-4 11 6.119105771365732E-4 1.7483159346759234E-4 1.7483159346759234E-4 12 6.119105771365732E-4 1.7483159346759234E-4 1.7483159346759234E-4 13 6.119105771365732E-4 1.7483159346759234E-4 1.7483159346759234E-4 14 6.993263738703693E-4 3.496631869351847E-4 1.7483159346759234E-4 15 6.993263738703693E-4 3.496631869351847E-4 1.7483159346759234E-4 16 6.993263738703693E-4 3.496631869351847E-4 1.7483159346759234E-4 17 6.993263738703693E-4 3.496631869351847E-4 1.7483159346759234E-4 18 6.993263738703693E-4 3.496631869351847E-4 2.622473902013885E-4 19 6.993263738703693E-4 3.496631869351847E-4 2.622473902013885E-4 20 6.993263738703693E-4 3.496631869351847E-4 3.496631869351847E-4 21 6.993263738703693E-4 3.496631869351847E-4 6.119105771365732E-4 22 6.993263738703693E-4 3.496631869351847E-4 0.0023602265118124965 23 6.993263738703693E-4 3.496631869351847E-4 0.002447642308546293 24 6.993263738703693E-4 4.3707898366898083E-4 0.002622473902013885 25 8.741579673379617E-4 4.3707898366898083E-4 0.0028847212922152735 26 8.741579673379617E-4 5.24494780402777E-4 0.0034092160726180505 27 8.741579673379617E-4 5.24494780402777E-4 0.010140232421120356 28 8.741579673379617E-4 5.24494780402777E-4 0.02395192830506015 29 0.001048989560805554 5.24494780402777E-4 0.032081597401303195 30 0.001048989560805554 5.24494780402777E-4 0.039774187513877256 31 0.001048989560805554 5.24494780402777E-4 0.04668003545584715 32 0.0011364053575393502 5.24494780402777E-4 0.055071951942291586 33 0.0011364053575393502 5.24494780402777E-4 0.06800948985889342 34 0.0011364053575393502 5.24494780402777E-4 0.09510838684637023 35 0.0011364053575393502 6.119105771365732E-4 0.1231688575979188 36 0.0012238211542731465 6.119105771365732E-4 0.16993630885049976 37 0.0012238211542731465 6.119105771365732E-4 0.24336557810688855 38 0.0012238211542731465 6.119105771365732E-4 0.323788111101981 39 0.0012238211542731465 6.993263738703693E-4 0.4219560508340341 40 0.0012238211542731465 6.993263738703693E-4 0.5522055879673904 41 0.0012238211542731465 6.993263738703693E-4 0.7085924483241518 42 0.0012238211542731465 6.993263738703693E-4 0.8883193264088367 43 0.0012238211542731465 6.993263738703693E-4 1.1002152176915585 44 0.0012238211542731465 6.993263738703693E-4 1.3489131593992087 45 0.0012238211542731465 6.993263738703693E-4 1.6319655092232408 46 0.0013112369510069426 6.993263738703693E-4 1.949634514553856 47 0.0013112369510069426 6.993263738703693E-4 2.248072044603036 48 0.0013112369510069426 6.993263738703693E-4 2.5202848356320775 49 0.0013112369510069426 7.867421706041656E-4 2.792410210864385 50 0.0013112369510069426 7.867421706041656E-4 3.075462560688417 51 0.0013112369510069426 7.867421706041656E-4 3.398201682229592 52 0.0013112369510069426 7.867421706041656E-4 3.784055009012569 53 0.0013986527477407387 7.867421706041656E-4 4.175677778379976 54 0.0013986527477407387 7.867421706041656E-4 4.617040136088912 55 0.0013986527477407387 7.867421706041656E-4 5.089435101638347 56 0.0013986527477407387 8.741579673379617E-4 5.55072826100259 57 0.0013986527477407387 8.741579673379617E-4 5.990954213353986 58 0.0013986527477407387 0.001048989560805554 6.419903527926724 59 0.0013986527477407387 0.0011364053575393502 6.818257313642634 60 0.0013986527477407387 0.0011364053575393502 7.221856047162571 61 0.0013986527477407387 0.0011364053575393502 7.621171406642552 62 0.0014860685444745348 0.0011364053575393502 8.073285907349746 63 0.0015734843412083311 0.0011364053575393502 8.561153468921061 64 0.0016609001379421272 0.0011364053575393502 9.14876245456564 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACG 110 2.9234445E-5 22.272726 4 CTCTTAG 195 7.8034645E-10 21.538462 1 CCGTGCA 140 8.251196E-6 20.0 9 GGACCGC 105 5.4665393E-4 20.0 6 GCTACAC 225 4.629328E-9 18.666666 3 CTAGCAC 150 1.496462E-5 18.666666 3 GGATACG 190 3.0070078E-7 18.421051 1 GTCTTAG 240 1.0293661E-8 17.500002 1 CCGAGGC 385 0.0 17.272726 70 GGAGTGC 185 4.749725E-6 17.027027 8 ACTGTGC 310 4.9112714E-11 16.935484 8 GTCTTAT 310 4.9112714E-11 16.935484 1 AGGACCG 145 2.4393082E-4 16.896551 5 ACTGTCC 250 1.704575E-8 16.800001 8 TATACCG 210 8.582301E-7 16.666666 5 TCTTAGG 275 3.07773E-9 16.545454 2 TACACTG 430 0.0 16.27907 5 ATGCCGT 265 3.4942786E-8 15.849057 14 TTAGGAC 155 4.055125E-4 15.806452 3 GTCTAGC 225 1.7628536E-6 15.555555 1 >>END_MODULE