FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve17.3410000000c889.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve17.3410000000c889.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143958
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC17770.1553378707959558No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15720.13741763246552757No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG1102.9234445E-522.2727264
CTCTTAG1957.8034645E-1021.5384621
CCGTGCA1408.251196E-620.09
GGACCGC1055.4665393E-420.06
GCTACAC2254.629328E-918.6666663
CTAGCAC1501.496462E-518.6666663
GGATACG1903.0070078E-718.4210511
GTCTTAG2401.0293661E-817.5000021
CCGAGGC3850.017.27272670
GGAGTGC1854.749725E-617.0270278
ACTGTGC3104.9112714E-1116.9354848
GTCTTAT3104.9112714E-1116.9354841
AGGACCG1452.4393082E-416.8965515
ACTGTCC2501.704575E-816.8000018
TATACCG2108.582301E-716.6666665
TCTTAGG2753.07773E-916.5454542
TACACTG4300.016.279075
ATGCCGT2653.4942786E-815.84905714
TTAGGAC1554.055125E-415.8064523
GTCTAGC2251.7628536E-615.5555551
Produced by FastQC (version 0.11.2)