##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve16.3410000000c86f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1319454 Sequences flagged as poor quality 0 Sequence length 76 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.82788259386079 34.0 34.0 34.0 34.0 34.0 2 33.85145598103458 34.0 34.0 34.0 34.0 34.0 3 33.86749064385723 34.0 34.0 34.0 34.0 34.0 4 33.87860887912728 34.0 34.0 34.0 34.0 34.0 5 33.883851956945826 34.0 34.0 34.0 34.0 34.0 6 37.7399674410779 38.0 38.0 38.0 38.0 38.0 7 37.7528833896445 38.0 38.0 38.0 38.0 38.0 8 37.69480482078193 38.0 38.0 38.0 38.0 38.0 9 37.682373921334126 38.0 38.0 38.0 38.0 38.0 10-11 37.67986682370132 38.0 38.0 38.0 38.0 38.0 12-13 37.632479419517466 38.0 38.0 38.0 37.5 38.0 14-15 37.668790651284546 38.0 38.0 38.0 38.0 38.0 16-17 37.6362514343054 38.0 38.0 38.0 37.5 38.0 18-19 37.651373219528686 38.0 38.0 38.0 38.0 38.0 20-21 37.619130716190185 38.0 38.0 38.0 37.0 38.0 22-23 37.655170244661804 38.0 38.0 38.0 38.0 38.0 24-25 37.6102353700849 38.0 38.0 38.0 37.0 38.0 26-27 37.58633874314679 38.0 38.0 38.0 37.0 38.0 28-29 37.53717522550994 38.0 38.0 38.0 37.0 38.0 30-31 37.55568439672774 38.0 38.0 38.0 37.0 38.0 32-33 37.526675048921746 38.0 38.0 38.0 37.0 38.0 34-35 37.55042237167798 38.0 38.0 38.0 37.0 38.0 36-37 37.48682788486752 38.0 38.0 38.0 37.0 38.0 38-39 37.52760232641684 38.0 38.0 38.0 37.0 38.0 40-41 36.33220748885524 38.0 38.0 38.0 35.0 38.0 42-43 37.12364432560741 38.0 38.0 38.0 36.0 38.0 44-45 37.33323291300796 38.0 38.0 38.0 37.0 38.0 46-47 36.94860904586291 38.0 38.0 38.0 36.0 38.0 48-49 37.24534277057025 38.0 38.0 38.0 36.5 38.0 50-51 37.30797322225709 38.0 38.0 38.0 37.0 38.0 52-53 37.31934913987149 38.0 38.0 38.0 37.0 38.0 54-55 37.316872357808606 38.0 38.0 38.0 37.0 38.0 56-57 37.31937036077044 38.0 38.0 38.0 37.0 38.0 58-59 37.302347789312854 38.0 38.0 38.0 37.0 38.0 60-61 37.30826538856223 38.0 38.0 38.0 37.0 38.0 62-63 37.30994070274522 38.0 38.0 38.0 37.0 38.0 64-65 37.24939861488161 38.0 38.0 38.0 37.0 38.0 66-67 37.236267804713165 38.0 38.0 38.0 37.0 38.0 68-69 37.22643722327569 38.0 38.0 38.0 37.0 38.0 70-71 37.17861175910642 38.0 38.0 38.0 37.0 38.0 72-73 37.21428825862819 38.0 38.0 38.0 37.0 38.0 74-75 37.21161442536079 38.0 38.0 38.0 37.0 38.0 76 36.68055801869561 38.0 37.0 38.0 35.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -7.46582318505773E-4 1101 2 0.029893919154638127 1101 3 0.017550010145114925 1101 4 0.018718034485452506 1101 5 0.014207637039952203 1101 6 0.057088073367921766 1101 7 7.242006196577222E-4 1101 8 0.03380639354273285 1101 9 0.020189486570117765 1101 10-11 0.022886820947960018 1101 12-13 0.028755543663827154 1101 14-15 0.05086123536244003 1101 16-17 0.010184567611794648 1101 18-19 0.040073912166725734 1101 20-21 0.09382811815932968 1101 22-23 0.0294015164353425 1101 24-25 -0.011128515659152072 1101 26-27 0.008005576966489514 1101 28-29 0.0462392592708909 1101 30-31 0.042098955969969154 1101 32-33 0.059676274886442116 1101 34-35 0.014512161147195002 1101 36-37 0.018282043772167356 1101 38-39 0.07978603132798412 1101 40-41 1.243901214026799 1101 42-43 0.37407931157039087 1101 44-45 0.0647617108540075 1101 46-47 0.5161374572965869 1101 48-49 0.196293570053804 1101 50-51 0.05606577542620528 1101 52-53 0.09921095254782841 1101 54-55 0.08906175994527388 1101 56-57 0.018299562231369748 1101 58-59 0.0011798453715456958 1101 60-61 0.02818888268077302 1101 62-63 0.11140105257985766 1101 64-65 0.0037350899506094493 1101 66-67 0.07191183728510708 1101 68-69 0.09291236606977549 1101 70-71 0.1100096169371696 1101 72-73 0.08716005273672067 1101 74-75 0.08635624986128931 1101 76 0.09699060760861755 1102 1 -0.023790502906926747 1102 2 0.018092903601903743 1102 3 -0.021100247711110853 1102 4 0.006625334287583939 1102 5 0.019145435435262925 1102 6 -0.03321037362600521 1102 7 0.004839463320898574 1102 8 -0.041458915274525054 1102 9 -0.010611461625885 1102 10-11 -0.009937538379517719 1102 12-13 0.056422861778976596 1102 14-15 -0.0075141653600780955 1102 16-17 0.05777668127260682 1102 18-19 0.021977084868709085 1102 20-21 0.07081850115235966 1102 22-23 0.01679317880607556 1102 24-25 -7.594141245519381E-4 1102 26-27 0.026889636029672204 1102 28-29 0.06325145984188651 1102 30-31 0.0563639106642313 1102 32-33 0.10223572386838242 1102 34-35 0.01088994469734672 1102 36-37 -0.10137489207021844 1102 38-39 -0.002949163696413848 1102 40-41 1.1943791383591815 1102 42-43 0.47411564085543034 1102 44-45 0.18156986555307952 1102 46-47 0.623839990976613 1102 48-49 0.2758506612168006 1102 50-51 0.147767834941547 1102 52-53 0.166356732848854 1102 54-55 0.13383781216074198 1102 56-57 0.12342886881052095 1102 58-59 0.17950086553921096 1102 60-61 0.1299998283492343 1102 62-63 0.11806841004201374 1102 64-65 0.09460077563210234 1102 66-67 0.11296301239325857 1102 68-69 0.08329773492122428 1102 70-71 0.12595523871646463 1102 72-73 0.16805244102624783 1102 74-75 0.1652292284091743 1102 76 0.1842428298804819 1103 1 -0.02210261852868456 1103 2 -0.047459931650081444 1103 3 -0.03062606828133596 1103 4 -0.00907121109095499 1103 5 -0.024765399642149077 1103 6 -0.03340750539567239 1103 7 0.00478646826339002 1103 8 0.020090660207166877 1103 9 0.03982826609436785 1103 10-11 0.04650133649767696 1103 12-13 0.0232190381937869 1103 14-15 -0.023419450558812116 1103 16-17 0.0038215600468234356 1103 18-19 0.07360760168700864 1103 20-21 0.058449446525116855 1103 22-23 0.04877754181860894 1103 24-25 0.04483618797477362 1103 26-27 0.020283301692877842 1103 28-29 0.0816455438871273 1103 30-31 0.0600509228389825 1103 32-33 0.053240398590304494 1103 34-35 0.05638427373398258 1103 36-37 -0.037566504806619605 1103 38-39 0.016755604611816466 1103 40-41 1.2342421272673008 1103 42-43 0.4380029546590194 1103 44-45 0.1551035923952213 1103 46-47 0.5496891178215222 1103 48-49 0.19623752349594525 1103 50-51 0.07645006443541291 1103 52-53 0.024816819531046974 1103 54-55 0.07338620239075055 1103 56-57 0.05834031456162592 1103 58-59 0.060266169953983706 1103 60-61 0.11349564157561787 1103 62-63 0.018295246936027354 1103 64-65 0.07321197862319195 1103 66-67 0.10111648411096752 1103 68-69 0.09587212488740704 1103 70-71 0.10821805548088292 1103 72-73 0.10757183163887873 1103 74-75 0.12872506788698246 1103 76 0.12896988728643777 2116 1 -0.009068291557909447 2116 2 -0.027072543091122725 2116 3 0.014977700123594673 2116 4 -0.024915224943114822 2116 5 0.01648139138573157 2116 6 -0.054246437873601394 2116 7 0.021248435020119416 2116 8 0.05234727201425926 2116 9 0.0661004158287426 2116 10-11 0.06408012188065726 2116 12-13 0.03896696349999473 2116 14-15 0.040168047815633656 2116 16-17 0.08197815306968437 2116 18-19 0.07667545127833364 2116 20-21 0.11125491899659323 2116 22-23 0.0699391776839633 2116 24-25 0.09571603635234993 2116 26-27 0.005719505159774485 2116 28-29 0.02480712195485779 2116 30-31 0.06946133893369222 2116 32-33 0.09365839580637925 2116 34-35 0.0283125709678842 2116 36-37 0.03498961909221521 2116 38-39 0.047076116506083565 2116 40-41 1.2108717793737895 2116 42-43 0.3681442939262425 2116 44-45 0.2093960290947834 2116 46-47 0.5746915260557515 2116 48-49 0.19815905359164532 2116 50-51 0.06976829814009022 2116 52-53 0.0796232938279644 2116 54-55 0.05644222755197603 2116 56-57 -0.006720300694055936 2116 58-59 0.06748184177908456 2116 60-61 0.08308433925071057 2116 62-63 0.06079961788115895 2116 64-65 0.13478667482443996 2116 66-67 0.13518806164888275 2116 68-69 0.12766057595931102 2116 70-71 0.14324233048196788 2116 72-73 0.12070947185831926 2116 74-75 0.028766369104097578 2116 76 0.17795409408632423 1117 1 -0.02836923575727468 1117 2 -0.03832167241547779 1117 3 -0.03132676547504332 1117 4 0.01487852167092285 1117 5 0.023707031816037727 1117 6 -0.032394414036438945 1117 7 0.016838946590432613 1117 8 0.003132814928896721 1117 9 0.020910415054807174 1117 10-11 0.029922548937392435 1117 12-13 0.018031567632796452 1117 14-15 0.022263086899293683 1117 16-17 0.07517382290948404 1117 18-19 0.08211620663782782 1117 20-21 0.021362979518222858 1117 22-23 0.07715079056221441 1117 24-25 0.013318195627945784 1117 26-27 0.08120466497712897 1117 28-29 0.09836276780270481 1117 30-31 0.07134151461669092 1117 32-33 0.05039065337157922 1117 34-35 0.039785657416295805 1117 36-37 0.08673048667212413 1117 38-39 0.06483644852373516 1117 40-41 1.2419539324579176 1117 42-43 0.38708148918976804 1117 44-45 0.1586719171839448 1117 46-47 0.5046775952454468 1117 48-49 0.26521945913301437 1117 50-51 0.11439719683214378 1117 52-53 0.09230807054803591 1117 54-55 0.08899727360104492 1117 56-57 0.08276435606625654 1117 58-59 0.10002818107171407 1117 60-61 0.16506038599631978 1117 62-63 0.07285218734173071 1117 64-65 0.16543832717332663 1117 66-67 0.07555113339255826 1117 68-69 0.12144878894592637 1117 70-71 0.14615715905561188 1117 72-73 0.0842092977231843 1117 74-75 0.054494350684471726 1117 76 0.2122616167238931 2117 1 -0.039962592691530574 2117 2 0.02677819806408621 2117 3 0.029344696423827088 2117 4 -0.005277853878830285 2117 5 0.03571492531686005 2117 6 -0.005930386688341116 2117 7 0.024200488465908165 2117 8 -0.012511533076086323 2117 9 0.05395656495950618 2117 10-11 -0.01634138162620502 2117 12-13 -0.03290131755463932 2117 14-15 -0.062773679566682 2117 16-17 0.031635231706260925 2117 18-19 0.05498775626382013 2117 20-21 0.06519375187926357 2117 22-23 0.06806159516750654 2117 24-25 0.08723517639138123 2117 26-27 0.08433006528067466 2117 28-29 0.07341334352816631 2117 30-31 0.09900204523643907 2117 32-33 0.07472627886731686 2117 34-35 0.04848053359553717 2117 36-37 0.1366024461442592 2117 38-39 -0.01790488033525861 2117 40-41 1.1971641702657223 2117 42-43 0.4381784179638757 2117 44-45 0.14315433197022998 2117 46-47 0.6281760121459783 2117 48-49 0.22920952642929393 2117 50-51 0.1284068506981555 2117 52-53 0.0781389422166967 2117 54-55 0.13139107751667467 2117 56-57 0.13804526564650388 2117 58-59 0.17940736444599992 2117 60-61 0.1542658570379558 2117 62-63 0.11205787673075918 2117 64-65 -0.13594285755697655 2117 66-67 0.03104582933006128 2117 68-69 0.12546112444137947 2117 70-71 0.22960492259606013 2117 72-73 0.14537523559478416 2117 74-75 0.12057439877912657 2117 76 0.3103519180885783 1116 1 -0.01593983599418891 1116 2 0.03159247666857823 1116 3 0.0036251698322757875 1116 4 0.04434849954651554 1116 5 0.03320385576898843 1116 6 0.014815238474206183 1116 7 0.024424135829903548 1116 8 0.023836864388890433 1116 9 0.06177696711653624 1116 10-11 0.02386435481307103 1116 12-13 -0.0116488779645465 1116 14-15 0.016160245673383145 1116 16-17 0.04540584972087203 1116 18-19 0.03851108487651089 1116 20-21 0.024840073306684474 1116 22-23 0.03321394726005167 1116 24-25 0.06360127110017544 1116 26-27 0.06827812455575355 1116 28-29 0.12689595955011868 1116 30-31 0.11136065505399273 1116 32-33 0.06847192651420642 1116 34-35 0.07041830684597272 1116 36-37 0.11880597624847411 1116 38-39 0.08819370820668837 1116 40-41 1.2199713377610877 1116 42-43 0.3387253055340764 1116 44-45 0.163026695901344 1116 46-47 0.5934935284148324 1116 48-49 0.30597407223857687 1116 50-51 0.1482689292424766 1116 52-53 0.11931828220740925 1116 54-55 0.09679566078070678 1116 56-57 0.09026932506382224 1116 58-59 0.13738278890956224 1116 60-61 0.10901430119380251 1116 62-63 0.14030119007323094 1116 64-65 -0.13947118375139667 1116 66-67 0.1050619842442515 1116 68-69 0.10029489584714213 1116 70-71 0.14164796239508348 1116 72-73 0.1664291034344174 1116 74-75 0.1729223133890443 1116 76 0.31195811279366126 2118 1 0.03735596299546273 2118 2 0.008359063877868778 2118 3 3.6070395625387164E-4 2118 4 0.00590480209039157 2118 5 -0.0029905180663050146 2118 6 0.04988562288349385 2118 7 0.05470097310448807 2118 8 0.020708615684498 2118 9 0.03332371547342916 2118 10-11 0.015538891014173828 2118 12-13 -0.014139745797656644 2118 14-15 0.039766357188888435 2118 16-17 0.06198487173853806 2118 18-19 0.06478110433135953 2118 20-21 0.03372144080685047 2118 22-23 0.044730033305640404 2118 24-25 0.018206700896996608 2118 26-27 0.08207129974321958 2118 28-29 0.0817873900345063 2118 30-31 0.11994474953966261 2118 32-33 0.06812090229871615 2118 34-35 0.09071355796098857 2118 36-37 0.14740410695652173 2118 38-39 0.1429282120795463 2118 40-41 1.2193917423972778 2118 42-43 0.41777607751839696 2118 44-45 0.14083820804010116 2118 46-47 0.6023528654374743 2118 48-49 0.2469153851031578 2118 50-51 0.09701539902654588 2118 52-53 0.15227263331769336 2118 54-55 0.08535049151466723 2118 56-57 0.15473034409166786 2118 58-59 0.03918302302589893 2118 60-61 0.1327075458188105 2118 62-63 0.06009328406656067 2118 64-65 0.13834883998298153 2118 66-67 0.09213096408205246 2118 68-69 0.05237884454405162 2118 70-71 0.12101596534534309 2118 72-73 0.11776334351109341 2118 74-75 0.12566009659933997 2118 76 0.2911766090921475 1119 1 -0.005882975710576943 1119 2 0.01740907615370446 1119 3 0.017672180784181535 1119 4 0.02129006943017231 1119 5 0.008447160744324833 1119 6 0.03532650994959852 1119 7 0.026921799541938185 1119 8 0.05063637905212204 1119 9 0.07344249216383503 1119 10-11 0.0650640291332536 1119 12-13 0.11099255339696867 1119 14-15 0.0717876121994081 1119 16-17 0.06397782712444666 1119 18-19 0.09050351835914228 1119 20-21 0.08294353903391283 1119 22-23 0.08050571525074446 1119 24-25 0.021551191144375537 1119 26-27 0.13788390938299955 1119 28-29 0.09952239920004757 1119 30-31 0.15202758251065518 1119 32-33 0.12913130400485784 1119 34-35 0.15309894901323418 1119 36-37 0.14755560702585058 1119 38-39 0.14301729936775587 1119 40-41 1.285545354801343 1119 42-43 0.4924013305087982 1119 44-45 0.21988379656161072 1119 46-47 0.6341960253782943 1119 48-49 0.27343179306553367 1119 50-51 0.15826690488395911 1119 52-53 0.20760681122448688 1119 54-55 0.17944916826829882 1119 56-57 0.20827637594270243 1119 58-59 0.12558397767908502 1119 60-61 0.1320173479170208 1119 62-63 0.15183883995851488 1119 64-65 0.16589223634500172 1119 66-67 0.12060888251959767 1119 68-69 0.1475585742720682 1119 70-71 0.2373289807874599 1119 72-73 0.1442641284015025 1119 74-75 0.1236209080513575 1119 76 0.3081455709378247 2119 1 -0.06100248803814878 2119 2 0.03535131659783275 2119 3 0.008580100662236134 2119 4 -0.044830282078898165 2119 5 0.019383198570004367 2119 6 0.02910636908833908 2119 7 0.012343625585742757 2119 8 0.029540814653621794 2119 9 0.06251766236803036 2119 10-11 -0.004026292433010781 2119 12-13 0.01584758779141282 2119 14-15 0.06370338653851348 2119 16-17 -0.0048615371813625075 2119 18-19 0.03798371645397225 2119 20-21 -0.002079667902307847 2119 22-23 0.030229374170502865 2119 24-25 0.0361023316482445 2119 26-27 0.06430635044770128 2119 28-29 0.05074150134529276 2119 30-31 0.06488889940049347 2119 32-33 0.08245376660161696 2119 34-35 0.050045810638586374 2119 36-37 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1.039012618408556 2104 42-43 0.18748571024688232 2104 44-45 -0.1231824487816695 2104 46-47 0.1991381295756227 2104 48-49 -0.0211975090946126 2104 50-51 -0.11276030755691124 2104 52-53 -0.09623967958831514 2104 54-55 -0.2992827292254603 2104 56-57 -0.04278564433184329 2104 58-59 -0.06071736617681722 2104 60-61 -0.11081389194932711 2104 62-63 -0.09554633988634009 2104 64-65 -0.1366869310942178 2104 66-67 -0.16607943055628738 2104 68-69 -0.23047856372712516 2104 70-71 -0.09056694352686634 2104 72-73 -0.0871053139499054 2104 74-75 -0.11583844620874828 2104 76 -0.24399089628403203 2111 1 -0.0801018690794919 2111 2 -0.08202210152281708 2111 3 -0.03641017949748715 2111 4 -0.03522743239184223 2111 5 -0.023784793451028463 2111 6 0.033835473210451994 2111 7 0.03119697872850935 2111 8 -0.0027204525832900117 2111 9 -0.0676901976533344 2111 10-11 -0.012095975166175776 2111 12-13 0.037377939532881044 2111 14-15 0.07471299351157512 2111 16-17 -0.003126527096078746 2111 18-19 -0.002347435642207074 2111 20-21 0.06942442361522438 2111 22-23 0.02720803353432899 2111 24-25 -0.013041680454740856 2111 26-27 0.06722974077197819 2111 28-29 0.036756961890439754 2111 30-31 0.03730410264719097 2111 32-33 0.015155636413638263 2111 34-35 0.04826677748699382 2111 36-37 0.0272356600346626 2111 38-39 -0.026082488020236383 2111 40-41 1.1813256552894913 2111 42-43 0.10907806850055124 2111 44-45 -0.05628635596325182 2111 46-47 0.4089829545897743 2111 48-49 0.05986732391392735 2111 50-51 -0.17849832531899779 2111 52-53 -0.12964849490009556 2111 54-55 -0.09348363218052924 2111 56-57 -0.20572914515333451 2111 58-59 -0.11853167040741397 2111 60-61 -0.1477683229249891 2111 62-63 -0.20046250151575862 2111 64-65 -0.10861893299482261 2111 66-67 0.002180094434258706 2111 68-69 -0.013299712353038728 2111 70-71 -0.023997327523545664 2111 72-73 -0.20624190652873153 2111 74-75 -0.3523389919082618 2111 76 0.08318450174323289 2110 1 0.026974882115084142 2110 2 0.02891275190059872 2110 3 0.01733316807666796 2110 4 0.00734125648928341 2110 5 0.0195089781354838 2110 6 0.07418608278072014 2110 7 0.047862267109294976 2110 8 0.029874745512636025 2110 9 0.00782037489760512 2110 10-11 -0.004975947991425755 2110 12-13 -0.012803211710327389 2110 14-15 0.004950243735692084 2110 16-17 0.006314909646697231 2110 18-19 -0.005169228878905585 2110 20-21 -0.11546585933754017 2110 22-23 -0.018498740482684184 2110 24-25 -0.028301487892285593 2110 26-27 0.02673647037634197 2110 28-29 -0.0418374566777473 2110 30-31 0.03053949202063677 2110 32-33 0.030532200040539692 2110 34-35 0.07008301123460825 2110 36-37 0.03205861729144033 2110 38-39 0.01478329021762903 2110 40-41 1.0687799782913885 2110 42-43 0.18439936011805003 2110 44-45 0.006378482360524629 2110 46-47 0.24727340840605194 2110 48-49 0.04193793871619533 2110 50-51 -0.09422696136619635 2110 52-53 -0.12668581174320082 2110 54-55 -0.10339510010111752 2110 56-57 -0.1239900108508536 2110 58-59 -0.14581567681100438 2110 60-61 -0.043790573401153665 2110 62-63 -0.12341008832388667 2110 64-65 -0.05512993682294365 2110 66-67 -0.2814095698713075 2110 68-69 -0.31253956572538755 2110 70-71 -0.1468217087528032 2110 72-73 -0.2844900513614306 2110 74-75 -0.1441914114259859 2110 76 -0.20666259418645438 2109 1 0.05245737674741946 2109 2 0.010627703931156418 2109 3 0.027717690829689445 2109 4 0.021090840554471413 2109 5 0.01311357276367886 2109 6 0.061536084688583514 2109 7 -0.018753277015491676 2109 8 0.0426265783952644 2109 9 -0.03187082197300839 2109 10-11 -0.08386849259338192 2109 12-13 -0.011596785368844564 2109 14-15 -0.018236824148559094 2109 16-17 -0.09956488016826626 2109 18-19 -0.07215547297631986 2109 20-21 -0.11422689673945996 2109 22-23 -0.10302288849844388 2109 24-25 -0.10525921250425796 2109 26-27 -0.0701102206699531 2109 28-29 -0.13316607245901224 2109 30-31 -0.11855685755907075 2109 32-33 -0.16316590042470125 2109 34-35 -0.020147208424099006 2109 36-37 -0.22428166517907044 2109 38-39 -0.0753912936731922 2109 40-41 -10.996095594119012 2109 42-43 -0.6638567088374714 2109 44-45 -0.1482983480911244 2109 46-47 0.29959002680171665 2109 48-49 0.10933008594911797 2109 50-51 -0.031867307309070725 2109 52-53 -0.020215897112926484 2109 54-55 -0.18706854604354106 2109 56-57 -0.11176224556650993 2109 58-59 -0.05930845831629483 2109 60-61 -0.1831109061818168 2109 62-63 -0.05636476199508422 2109 64-65 -0.18861196043860673 2109 66-67 -0.03157949283053796 2109 68-69 -0.2590674794128134 2109 70-71 -0.10500589022388596 2109 72-73 -0.035806046521607016 2109 74-75 -0.20688126950964403 2109 76 -0.03966642099181428 2108 1 0.08758010665852112 2108 2 0.04089674704212598 2108 3 -0.006591922518218496 2108 4 0.018461445123463704 2108 5 0.02141907856414349 2108 6 0.05451444714578457 2108 7 -0.06447801649202489 2108 8 -0.01807679028392073 2108 9 9.13688842025806E-4 2108 10-11 -0.09258562566985518 2108 12-13 -0.13151053767533227 2108 14-15 -0.06996337766030791 2108 16-17 -0.18875271508025548 2108 18-19 -0.19196585103605202 2108 20-21 -0.28640601173458435 2108 22-23 -0.0838299305781618 2108 24-25 -0.3315472668372266 2108 26-27 -0.20459338475289002 2108 28-29 -0.5963456449101088 2108 30-31 -0.2658083199847425 2108 32-33 -0.5007155152849947 2108 34-35 -0.2708243138953321 2108 36-37 -0.6226382722601258 2108 38-39 -0.1987763515241312 2108 40-41 -7.157752889378379 2108 42-43 -0.3654086105621417 2108 44-45 -0.06402191172714566 2108 46-47 0.5019755703620277 2108 48-49 0.15752525386786687 2108 50-51 0.09394638055471205 2108 52-53 -0.06018155259830138 2108 54-55 -0.09606854562805012 2108 56-57 -0.053056850120540844 2108 58-59 -0.021699469742728184 2108 60-61 -0.14623347322031321 2108 62-63 -0.026532358739125073 2108 64-65 0.02535540437817474 2108 66-67 -0.040955221238803574 2108 68-69 -0.014790145587539882 2108 70-71 0.009910664571741279 2108 72-73 -0.018767868268341203 2108 74-75 -0.09024611786153258 2108 76 -0.45601342939180256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 14.0 18 40.0 19 83.0 20 155.0 21 259.0 22 345.0 23 449.0 24 644.0 25 934.0 26 1292.0 27 1797.0 28 2552.0 29 3723.0 30 5311.0 31 7635.0 32 11513.0 33 17067.0 34 25995.0 35 43586.0 36 99208.0 37 1096849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.97694955640742 26.74257685375921 8.350802680502694 23.929670909330678 2 15.099958012935655 23.287587138316304 41.194009037071396 20.418445811676648 3 18.63111559781546 26.04736504645103 32.23636443559229 23.08515492014121 4 13.412517601977788 16.347443715354988 41.45692081724713 28.78311786542009 5 12.439160440606493 35.9309987312934 37.64178213109362 13.988058697006489 6 27.11583730846244 39.01022695751424 18.698188796274824 15.175746937748494 7 23.18231632175127 35.067914455524786 23.674186443786596 18.07558277893735 8 20.188350635944868 41.78258582716791 22.47573617572117 15.553327361166058 9 20.79572307939496 18.130150804802593 25.32479343728542 35.74933267851702 10-11 23.360799239685505 26.943265926663607 30.60133206614251 19.094602767508377 12-13 20.488020044654835 25.20368273543451 31.582040753220653 22.72625646669001 14-15 19.860449852742118 29.166875086209902 25.689792899184056 25.282882161863924 16-17 19.066106131778753 34.17629564956414 27.86076665044784 18.896831568209276 18-19 18.631570331364337 32.38582019532322 30.621188764443474 18.361420708868973 20-21 18.87826328163013 30.323300395466607 31.190742534411964 19.6076937884913 22-23 19.21662293645705 30.540814609679458 30.941283288390505 19.30127916547299 24-25 19.14909500444881 31.01085752136869 30.805772690825144 19.034274783357358 26-27 18.855109765099805 31.29434599463111 30.706110254696263 19.14443398557282 28-29 18.654572270045033 31.30749537308614 31.03063843074484 19.00729392612399 30-31 18.865189692099914 30.932757034349056 31.108435762065216 19.09361751148581 32-33 18.91145883069815 31.05030565673377 30.851549201412098 19.186686311155977 34-35 18.758554674888252 30.93984329881906 31.314657426480952 18.98694459981174 36-37 18.93953862734131 31.19464566403982 31.12939139977597 18.7364243088429 38-39 18.81929949812574 31.01498801777099 30.994600796996334 19.17111168710694 40-41 18.79326600245253 30.95852526878542 31.022225860090614 19.225982868671434 42-43 19.02885587523324 30.975994615954782 30.950302170443226 19.04484733836875 44-45 18.66844164328578 30.67640099616963 31.327844699398387 19.327312661146202 46-47 18.69936352460942 30.61929404132315 31.616600502935306 19.06474193113212 48-49 18.63695134502605 30.86193228411146 31.394160008609624 19.106956362252873 50-51 18.51796273306989 30.79501066350172 31.51629386094551 19.170732742482876 52-53 18.329399888135548 30.69424928796305 31.764237328470717 19.212113495430685 54-55 18.34501240664699 30.47063406530277 31.866476588043234 19.317876940007004 56-57 18.218899635758426 30.505724337491113 31.836236807042912 19.439139219707545 58-59 17.98410554668825 30.675226267835026 31.998614578454422 19.3420536070223 60-61 18.25273939068736 30.37453370864009 31.811908562177994 19.56081833849456 62-63 18.01555795048558 30.050270793828354 31.96962531471351 19.964545940972553 64-65 17.967356194304614 29.860419537172195 31.927941406066452 20.244282862456746 66-67 17.97451824769943 29.921884355195406 31.622739405845145 20.48085799126002 68-69 17.894560932021882 30.007639523621133 31.44823540646358 20.6495641378934 70-71 17.92445966286055 29.990283859838996 31.420345082132457 20.664911395168 72-73 17.773753385870215 29.8398049496231 31.38980214543288 20.9966395190738 74-75 17.928817526037285 29.688606044621483 31.030827903056867 21.351748526284357 76 17.877470529476586 29.525243017187414 31.02472689460944 21.572559558726564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 6.0 2 8.0 3 9.5 4 7.0 5 5.0 6 3.5 7 11.5 8 19.0 9 34.5 10 92.5 11 234.0 12 333.0 13 495.0 14 824.5 15 1431.5 16 2457.0 17 3043.0 18 4099.5 19 6400.5 20 9394.5 21 11144.0 22 13248.0 23 18094.0 24 23817.5 25 26799.0 26 29934.0 27 36410.5 28 42249.5 29 44747.0 30 48127.0 31 53768.5 32 58186.0 33 60342.0 34 61412.5 35 64680.5 36 67976.0 37 69074.0 38 69505.0 39 70782.5 40 71272.5 41 70053.5 42 69191.0 43 68035.5 44 64912.0 45 60379.5 46 57815.0 47 54563.5 48 47821.0 49 41235.0 50 38140.0 51 35312.5 52 28934.5 53 22859.0 54 20334.0 55 18101.5 56 14071.0 57 10600.0 58 8927.0 59 7957.0 60 6041.0 61 4295.5 62 3496.0 63 3019.0 64 2114.5 65 1417.0 66 960.0 67 773.0 68 639.5 69 421.5 70 260.5 71 184.0 72 183.5 73 137.5 74 70.5 75 49.0 76 36.0 77 20.0 78 13.0 79 9.0 80 6.5 81 2.5 82 1.5 83 2.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1319454.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.63196405444329 #Duplication Level Percentage of deduplicated Percentage of total 1 95.49648906943946 87.50530853736413 2 3.411238049009309 6.2515688457594045 3 0.47446533123527396 1.3042857053049048 4 0.16130554747819442 0.5912297651321684 5 0.12668259678227797 0.5804087577338614 6 0.06675629511392674 0.3670206260572286 7 0.05343504838499413 0.34274509030028544 8 0.03647568452287648 0.2673870890449145 9 0.027875101339201416 0.22988252555349017 >10 0.14125923944185068 2.234717996929979 >50 0.002770461571945811 0.14815930510525224 >100 0.0012475756806900034 0.17728575571436647 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.515778496256785E-4 7.578892481283925E-5 0.0 2 1.515778496256785E-4 7.578892481283925E-5 0.0 3 1.515778496256785E-4 7.578892481283925E-5 0.0 4 1.515778496256785E-4 7.578892481283925E-5 0.0 5 1.515778496256785E-4 7.578892481283925E-5 0.0 6 1.515778496256785E-4 7.578892481283925E-5 0.0 7 1.515778496256785E-4 7.578892481283925E-5 0.0 8 1.515778496256785E-4 7.578892481283925E-5 0.0 9 1.515778496256785E-4 7.578892481283925E-5 0.0 10 1.515778496256785E-4 7.578892481283925E-5 7.578892481283925E-5 11 1.515778496256785E-4 1.515778496256785E-4 7.578892481283925E-5 12 1.515778496256785E-4 1.515778496256785E-4 7.578892481283925E-5 13 1.515778496256785E-4 2.2736677443851774E-4 7.578892481283925E-5 14 1.515778496256785E-4 2.2736677443851774E-4 7.578892481283925E-5 15 2.2736677443851774E-4 2.2736677443851774E-4 7.578892481283925E-5 16 2.2736677443851774E-4 2.2736677443851774E-4 7.578892481283925E-5 17 2.2736677443851774E-4 2.2736677443851774E-4 7.578892481283925E-5 18 2.2736677443851774E-4 2.2736677443851774E-4 7.578892481283925E-5 19 3.03155699251357E-4 2.2736677443851774E-4 7.578892481283925E-5 20 3.03155699251357E-4 3.03155699251357E-4 7.578892481283925E-5 21 3.03155699251357E-4 3.03155699251357E-4 2.2736677443851774E-4 22 3.03155699251357E-4 3.03155699251357E-4 6.06311398502714E-4 23 3.03155699251357E-4 3.03155699251357E-4 6.821003233155533E-4 24 3.03155699251357E-4 3.03155699251357E-4 8.336781729412318E-4 25 3.03155699251357E-4 3.03155699251357E-4 9.852560225669103E-4 26 3.7894462406419624E-4 3.03155699251357E-4 0.0014399895714439458 27 3.7894462406419624E-4 3.03155699251357E-4 0.004698913338396033 28 3.7894462406419624E-4 3.03155699251357E-4 0.013263061842246869 29 3.7894462406419624E-4 3.03155699251357E-4 0.022585099594226096 30 3.7894462406419624E-4 3.7894462406419624E-4 0.031603981646953966 31 6.06311398502714E-4 3.7894462406419624E-4 0.03819761810567098 32 7.578892481283925E-4 3.7894462406419624E-4 0.0461554552110191 33 7.578892481283925E-4 3.7894462406419624E-4 0.05578064866224969 34 7.578892481283925E-4 3.7894462406419624E-4 0.07093843362481754 35 7.578892481283925E-4 3.7894462406419624E-4 0.09246248827166388 36 7.578892481283925E-4 3.7894462406419624E-4 0.13543480864054375 37 7.578892481283925E-4 4.547335488770355E-4 0.19940066118258007 38 7.578892481283925E-4 4.547335488770355E-4 0.28292005632632894 39 8.336781729412318E-4 4.547335488770355E-4 0.3803846136356402 40 8.336781729412318E-4 4.547335488770355E-4 0.4999037480654877 41 8.336781729412318E-4 4.547335488770355E-4 0.6218481280893461 42 8.336781729412318E-4 5.305224736898747E-4 0.7631186839404784 43 8.336781729412318E-4 5.305224736898747E-4 0.9056018625886162 44 8.336781729412318E-4 5.305224736898747E-4 1.074535375996435 45 8.336781729412318E-4 5.305224736898747E-4 1.2617340202841478 46 8.336781729412318E-4 5.305224736898747E-4 1.4937997080610617 47 9.09467097754071E-4 5.305224736898747E-4 1.7365516342365857 48 0.0010610449473797494 5.305224736898747E-4 2.003783383126657 49 0.0010610449473797494 5.305224736898747E-4 2.2879918511748043 50 0.0010610449473797494 5.305224736898747E-4 2.5939517406442363 51 0.0010610449473797494 5.305224736898747E-4 2.903852654203936 52 0.0010610449473797494 5.305224736898747E-4 3.2223177162674865 53 0.0010610449473797494 5.305224736898747E-4 3.5366143874663307 54 0.0010610449473797494 6.06311398502714E-4 3.8644014872818606 55 0.0010610449473797494 6.06311398502714E-4 4.208104261308087 56 0.001212622797005428 6.06311398502714E-4 4.5741647681541 57 0.001212622797005428 6.06311398502714E-4 4.962583007819902 58 0.001212622797005428 6.06311398502714E-4 5.37684527084688 59 0.0012884117218182672 6.06311398502714E-4 5.805204273889048 60 0.0012884117218182672 6.06311398502714E-4 6.269032493743624 61 0.0014399895714439458 6.06311398502714E-4 6.729147056282371 62 0.0014399895714439458 6.06311398502714E-4 7.205556237655879 63 0.0014399895714439458 6.06311398502714E-4 7.662260298578048 64 0.0014399895714439458 6.06311398502714E-4 8.132151632417651 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACGC 335 0.0 20.895521 70 TAGACTG 270 5.456968E-12 19.444445 5 GTGTAAG 375 0.0 18.666668 1 ACCGCGC 150 1.4967465E-5 18.666666 8 TACACTG 555 0.0 18.288288 5 ACCGCGT 115 0.0010081254 18.260868 8 GTATACA 445 0.0 18.089888 1 GCACTGC 255 1.1277734E-9 17.843138 6 TAAGGTC 160 2.6078871E-5 17.500002 4 GTCCAGG 220 7.274866E-8 17.500002 1 ACCGGGT 120 0.0013412109 17.5 8 GGCGTAC 120 0.0013412109 17.5 1 GGACCGC 140 1.8659409E-4 17.5 6 ACCCCGC 120 0.0013412109 17.5 8 CCTATAC 245 1.3287718E-8 17.142857 3 GGGCTAT 145 2.4396654E-4 16.896551 1 ACCGTGC 145 2.4396654E-4 16.896551 8 ACACCGT 270 2.413799E-9 16.851852 6 GTCCTAG 250 1.7053026E-8 16.8 1 CACTGTG 380 0.0 16.578947 7 >>END_MODULE