FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve16.3410000000c86f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve16.3410000000c86f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319454
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGC3350.020.89552170
TAGACTG2705.456968E-1219.4444455
GTGTAAG3750.018.6666681
ACCGCGC1501.4967465E-518.6666668
TACACTG5550.018.2882885
ACCGCGT1150.001008125418.2608688
GTATACA4450.018.0898881
GCACTGC2551.1277734E-917.8431386
TAAGGTC1602.6078871E-517.5000024
GTCCAGG2207.274866E-817.5000021
ACCGGGT1200.001341210917.58
GGCGTAC1200.001341210917.51
GGACCGC1401.8659409E-417.56
ACCCCGC1200.001341210917.58
CCTATAC2451.3287718E-817.1428573
GGGCTAT1452.4396654E-416.8965511
ACCGTGC1452.4396654E-416.8965518
ACACCGT2702.413799E-916.8518526
GTCCTAG2501.7053026E-816.81
CACTGTG3800.016.5789477
Produced by FastQC (version 0.11.2)