##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve15.3410000000c846.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1210567 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.79441617027393 34.0 34.0 34.0 34.0 34.0 2 33.81903356030686 34.0 34.0 34.0 34.0 34.0 3 33.83919188281194 34.0 34.0 34.0 34.0 34.0 4 33.854510324500836 34.0 34.0 34.0 34.0 34.0 5 33.856969502720624 34.0 34.0 34.0 34.0 34.0 6 37.69090269270515 38.0 38.0 38.0 38.0 38.0 7 37.707899686675745 38.0 38.0 38.0 38.0 38.0 8 37.636006928984514 38.0 38.0 38.0 38.0 38.0 9 37.622303433019404 38.0 38.0 38.0 37.0 38.0 10-11 37.61419772718074 38.0 38.0 38.0 37.0 38.0 12-13 37.56325383064299 38.0 38.0 38.0 37.0 38.0 14-15 37.60422017120903 38.0 38.0 38.0 37.0 38.0 16-17 37.56350619172669 38.0 38.0 38.0 37.0 38.0 18-19 37.58216769497269 38.0 38.0 38.0 37.0 38.0 20-21 37.54430898909354 38.0 38.0 38.0 37.0 38.0 22-23 37.58424688596335 38.0 38.0 38.0 37.0 38.0 24-25 37.53294571882432 38.0 38.0 38.0 37.0 38.0 26-27 37.50545942521149 38.0 38.0 38.0 37.0 38.0 28-29 37.45138682947743 38.0 38.0 38.0 37.0 38.0 30-31 37.473460783252804 38.0 38.0 38.0 37.0 38.0 32-33 37.434131278979194 38.0 38.0 38.0 37.0 38.0 34-35 37.46419074698055 38.0 38.0 38.0 37.0 38.0 36-37 37.38994950300149 38.0 38.0 38.0 37.0 38.0 38-39 37.4399578875023 38.0 38.0 38.0 37.0 38.0 40-41 36.24198619324663 38.0 37.5 38.0 35.0 38.0 42-43 37.00903502243163 38.0 37.5 38.0 35.5 38.0 44-45 37.2128626503118 38.0 38.0 38.0 37.0 38.0 46-47 36.813451878334696 38.0 38.0 38.0 35.5 38.0 48-49 37.11950061417501 38.0 38.0 38.0 36.5 38.0 50-51 37.181469922771726 38.0 38.0 38.0 37.0 38.0 52-53 37.193655947997925 38.0 38.0 38.0 37.0 38.0 54-55 37.18575716998728 38.0 38.0 38.0 37.0 38.0 56-57 37.192272298848394 38.0 38.0 38.0 37.0 38.0 58-59 37.17194835147497 38.0 38.0 38.0 37.0 38.0 60-61 37.173159354253 38.0 38.0 38.0 37.0 38.0 62-63 37.17692453205812 38.0 38.0 38.0 37.0 38.0 64-65 37.110943880016556 38.0 38.0 38.0 36.5 38.0 66-67 37.092103121925504 38.0 38.0 38.0 37.0 38.0 68-69 37.083642623663124 38.0 38.0 38.0 36.0 38.0 70-71 37.02439600616901 38.0 38.0 38.0 36.5 38.0 72-73 37.06323648339993 38.0 38.0 38.0 36.0 38.0 74-75 37.0629754486947 38.0 38.0 38.0 36.0 38.0 76 36.49399578875023 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.016065335427050798 1101 2 0.06011231720023602 1101 3 0.024607166025504057 1101 4 0.019661739262851086 1101 5 -0.008454316871606693 1101 6 0.06666086187408382 1101 7 0.0038594242159817327 1101 8 0.046844771661092466 1101 9 0.03074469479585673 1101 10-11 0.023730198907259137 1101 12-13 0.060193541825732666 1101 14-15 0.029657719955885398 1101 16-17 0.12647277380410316 1101 18-19 0.0745570020362365 1101 20-21 0.03892103795013213 1101 22-23 -0.0305040117425861 1101 24-25 -0.010733688803220787 1101 26-27 -0.005085821787034206 1101 28-29 0.007606869324561671 1101 30-31 0.08133285873479679 1101 32-33 0.138246047174583 1101 34-35 0.07972292173660378 1101 36-37 0.12202711763584517 1101 38-39 0.11213853018735165 1101 40-41 1.2580900562340886 1101 42-43 0.5209813182068928 1101 44-45 0.238501894475192 1101 46-47 0.6009912651563027 1101 48-49 0.26104145324141115 1101 50-51 0.23725070491505562 1101 52-53 0.18040544026063543 1101 54-55 0.15760227074572697 1101 56-57 0.07009975323321527 1101 58-59 0.09147095771957225 1101 60-61 0.15868435595685781 1101 62-63 0.04870088953892093 1101 64-65 0.10112330176634998 1101 66-67 0.19077531846771478 1101 68-69 0.11317080497276066 1101 70-71 0.1682678878002477 1101 72-73 0.13484645745103307 1101 74-75 0.18214449078394068 1101 76 0.17960382632639948 1102 1 -0.08966108980065712 1102 2 0.013913914479743994 1102 3 0.03485665208558686 1102 4 0.041634703734530376 1102 5 0.03763248468379743 1102 6 0.025878621456350004 1102 7 0.04584074416756323 1102 8 0.006336372845659355 1102 9 -0.015182495669158413 1102 10-11 0.07104393159617928 1102 12-13 0.1220903623611278 1102 14-15 0.030024562529099796 1102 16-17 0.12024862296139815 1102 18-19 0.08908453275820705 1102 20-21 0.12019541418628421 1102 22-23 0.05392056544383905 1102 24-25 0.08480129076090748 1102 26-27 0.12166890202929181 1102 28-29 0.08938310996238386 1102 30-31 0.0774163979524829 1102 32-33 0.11480766007435506 1102 34-35 0.024275742879666495 1102 36-37 -0.06185596018016071 1102 38-39 0.06109205015236796 1102 40-41 1.2335708128237712 1102 42-43 0.5243352935358274 1102 44-45 0.24568634120705468 1102 46-47 0.7110914627468432 1102 48-49 0.3080952214362469 1102 50-51 0.21931222968301967 1102 52-53 0.25664193929507206 1102 54-55 0.2754476476236434 1102 56-57 0.1568016856083645 1102 58-59 0.25325681008077083 1102 60-61 0.2471689854770247 1102 62-63 0.15226136597655682 1102 64-65 0.19012820111593243 1102 66-67 0.2631049976581892 1102 68-69 0.16904043221902754 1102 70-71 0.2695404429702748 1102 72-73 0.2300475586604307 1102 74-75 0.25509401414178967 1102 76 0.43715341142520714 1103 1 -0.010801175014101716 1103 2 -0.04038794751200925 1103 3 0.012213101515889946 1103 4 0.0020939813110274486 1103 5 0.011028581676434612 1103 6 0.01704384181126528 1103 7 0.02867800429832812 1103 8 0.016902992937403383 1103 9 0.03820375979789503 1103 10-11 0.05002767919742723 1103 12-13 0.05340618294858501 1103 14-15 0.023228883801301947 1103 16-17 0.07383994212312928 1103 18-19 0.06297586527492172 1103 20-21 0.06078625036653307 1103 22-23 0.038035404942242224 1103 24-25 0.08516355080790561 1103 26-27 0.06048606170565307 1103 28-29 0.12730060293904444 1103 30-31 0.14354939161915325 1103 32-33 0.1542364207840805 1103 34-35 0.0486372044993999 1103 36-37 0.03385163162820959 1103 38-39 0.05476125905359197 1103 40-41 1.2470886460415045 1103 42-43 0.49602956290546985 1103 44-45 0.1998363679888442 1103 46-47 0.6245291660334615 1103 48-49 0.24696800296442945 1103 50-51 0.13007529734780832 1103 52-53 0.13294789202991097 1103 54-55 0.14662466249171757 1103 56-57 0.08017712784277364 1103 58-59 0.18856725612302938 1103 60-61 0.16918274542325662 1103 62-63 0.0933531817619695 1103 64-65 0.09715565351545052 1103 66-67 0.09563535632339892 1103 68-69 0.1465165500052379 1103 70-71 0.14187910250279145 1103 72-73 0.22170739226397984 1103 74-75 0.1584419518012794 1103 76 0.25236182344048785 2116 1 0.033436395878609915 2116 2 -0.0017180786323436337 2116 3 0.03083162522815286 2116 4 -0.006908692828496044 2116 5 0.022752887409488665 2116 6 -0.0523364163767468 2116 7 0.041571382579313365 2116 8 0.05635547361628568 2116 9 0.032270896511356284 2116 10-11 0.036846086395797784 2116 12-13 0.06855768937796114 2116 14-15 0.0036663232129328094 2116 16-17 0.0740495308782343 2116 18-19 0.0875881764483637 2116 20-21 0.062257928972414334 2116 22-23 0.005120471976105989 2116 24-25 0.06977232933864741 2116 26-27 0.05102731974860575 2116 28-29 0.03429236675818714 2116 30-31 0.00941028570588287 2116 32-33 0.08341345895196639 2116 34-35 -0.022368242025869733 2116 36-37 0.05325444615237984 2116 38-39 0.0325740178614069 2116 40-41 1.2267456323845067 2116 42-43 0.37162201452495935 2116 44-45 0.20709042237272968 2116 46-47 0.6119476523661049 2116 48-49 0.2446299538121508 2116 50-51 0.14458191899007744 2116 52-53 0.14198074042599984 2116 54-55 0.11435058468224213 2116 56-57 0.08381478078644022 2116 58-59 0.1488050215893253 2116 60-61 0.14615902011049542 2116 62-63 0.0915514572336491 2116 64-65 0.18843642550053374 2116 66-67 0.17254795325519723 2116 68-69 0.13106790630209986 2116 70-71 0.1464144515724044 2116 72-73 0.10145525636795583 2116 74-75 0.17098129826430153 2116 76 0.20444212975380793 1117 1 0.013652888846131361 1117 2 0.019058670303117253 1117 3 0.005789869512213386 1117 4 -0.003657091871119178 1117 5 0.01803395123089757 1117 6 -0.07397452167062113 1117 7 0.027542021596126176 1117 8 -0.0460304487643981 1117 9 0.013084395879616295 1117 10-11 0.045368864100254314 1117 12-13 0.060995833417429424 1117 14-15 0.007537747638558301 1117 16-17 0.02694743352181206 1117 18-19 0.04753635999752959 1117 20-21 0.056098285524555536 1117 22-23 0.05386800310186146 1117 24-25 0.06892414223908361 1117 26-27 0.05898999392049831 1117 28-29 0.12834484967963533 1117 30-31 0.09451051871975835 1117 32-33 0.08504692943854764 1117 34-35 0.054546886587132803 1117 36-37 0.1550434103713556 1117 38-39 0.027655854433703553 1117 40-41 1.2052214677110769 1117 42-43 0.3182941379922113 1117 44-45 0.13742933638451404 1117 46-47 0.6051248667890405 1117 48-49 0.24166489277239123 1117 50-51 0.09494814015952358 1117 52-53 0.08169963994291862 1117 54-55 0.08144711911218394 1117 56-57 0.0743536089713146 1117 58-59 0.06456252700560583 1117 60-61 0.12359554524767447 1117 62-63 0.12096270549800181 1117 64-65 0.17519852250192258 1117 66-67 0.08585390224839529 1117 68-69 0.10022289719070443 1117 70-71 0.1277581945053896 1117 72-73 0.020509852159499076 1117 74-75 0.031029703249977558 1117 76 0.25183284130670813 2117 1 0.025033494309916193 2117 2 0.013496293018519623 2117 3 -3.961263001386328E-4 2117 4 -0.016639433544270332 2117 5 0.007629155887094896 2117 6 -0.07319139294588695 2117 7 0.016008251729829226 2117 8 0.047172253505642914 2117 9 0.05365334827260426 2117 10-11 -0.04645809877655438 2117 12-13 -0.11640734057589697 2117 14-15 -0.1131996452148698 2117 16-17 0.0316375525141126 2117 18-19 0.033108907859968895 2117 20-21 0.048542896113431766 2117 22-23 0.026880348844976254 2117 24-25 0.030918169692718322 2117 26-27 0.04012062988736176 2117 28-29 0.06681285375132973 2117 30-31 6.24778210251975E-4 2117 32-33 0.0448591573806425 2117 34-35 0.012339276998872606 2117 36-37 0.1039410160742591 2117 38-39 6.670463960034567E-4 2117 40-41 1.1781889602252065 2117 42-43 0.4533335093201245 2117 44-45 0.25521737704259095 2117 46-47 0.6481931968615058 2117 48-49 0.30090072066636964 2117 50-51 0.09844236534235051 2117 52-53 0.09205366893107936 2117 54-55 0.19302176166367246 2117 56-57 0.17735234298532276 2117 58-59 0.2872214552643797 2117 60-61 0.19367676853223514 2117 62-63 0.21104313267380803 2117 64-65 -0.22605211820698656 2117 66-67 0.054212617317318745 2117 68-69 0.14155911382228226 2117 70-71 0.268096015757628 2117 72-73 0.25594719133327004 2117 74-75 0.1781328625037517 2117 76 0.3844374466805007 1116 1 -0.020792925336365897 1116 2 -0.010032678642943438 1116 3 0.02412996622506114 1116 4 0.011860886917858693 1116 5 0.017723115058721817 1116 6 0.022105596853187137 1116 7 0.04545689900076155 1116 8 -0.012879642320392293 1116 9 0.04538438243007192 1116 10-11 -0.00392135517888903 1116 12-13 -0.04784891883203812 1116 14-15 0.08012933683685475 1116 16-17 0.03450415540407192 1116 18-19 0.08092799060253242 1116 20-21 0.09072339527278928 1116 22-23 0.06213132680699829 1116 24-25 0.06275074140825865 1116 26-27 0.07284503255007024 1116 28-29 0.12873351404395805 1116 30-31 0.08418024408679514 1116 32-33 0.059121229101585016 1116 34-35 0.09391298668553816 1116 36-37 0.18856095774528114 1116 38-39 0.13637240225152425 1116 40-41 1.2606131694242606 1116 42-43 0.4236460582038788 1116 44-45 0.16528696788913777 1116 46-47 0.5530205840651874 1116 48-49 0.2673583000239219 1116 50-51 0.08868680729288059 1116 52-53 0.11513373528716642 1116 54-55 0.16130184681026094 1116 56-57 0.16333711177755816 1116 58-59 0.15814056208824923 1116 60-61 0.12196677705014736 1116 62-63 0.09243631392468643 1116 64-65 -0.15325818114576606 1116 66-67 0.0920558512035825 1116 68-69 0.11000165446940713 1116 70-71 0.13812778834135742 1116 72-73 0.19000054313563197 1116 74-75 0.17658967876054987 1116 76 0.3935619349528494 2118 1 -0.013584770717095296 2118 2 -0.03863037107198153 2118 3 -0.008597241629090036 2118 4 0.014120289593499535 2118 5 0.017148757490382138 2118 6 0.026113799546855887 2118 7 0.09363025977656037 2118 8 0.02449913884345989 2118 9 0.05424340426635865 2118 10-11 0.05152889157796636 2118 12-13 0.0024968506211919816 2118 14-15 0.0759836244719736 2118 16-17 0.08884333904075703 2118 18-19 0.08995649346070422 2118 20-21 0.07536198286208418 2118 22-23 0.10077576970738988 2118 24-25 0.05635351357318541 2118 26-27 0.0943249445889407 2118 28-29 0.1285608875294315 2118 30-31 0.12737870307708477 2118 32-33 0.03076231242803118 2118 34-35 0.072520678964338 2118 36-37 0.14740702202975342 2118 38-39 0.09870442355448716 2118 40-41 1.1552688365691708 2118 42-43 0.3868689581415268 2118 44-45 0.11715938107354162 2118 46-47 0.5421876012743283 2118 48-49 0.16039389761118628 2118 50-51 0.06209377958249007 2118 52-53 0.12130453459909774 2118 54-55 0.10495372621863908 2118 56-57 0.0816810145921707 2118 58-59 -0.07116449060579555 2118 60-61 0.1310147162360451 2118 62-63 0.08066860339263826 2118 64-65 0.09035768941392064 2118 66-67 0.08592533297662897 2118 68-69 0.06869335487578354 2118 70-71 0.037777887494797824 2118 72-73 0.08451475700660183 2118 74-75 0.05018040415810532 2118 76 0.26855395385209846 1119 1 -6.739620509961242E-4 1119 2 -0.008784513221137047 1119 3 0.011100987121153594 1119 4 -0.0484613106700138 1119 5 -0.041094553533888245 1119 6 0.001248926588736765 1119 7 -0.02339836769203174 1119 8 0.035064127090024044 1119 9 0.006920866123849123 1119 10-11 -0.0032177732919933533 1119 12-13 0.10143838665256766 1119 14-15 0.058787211140433726 1119 16-17 -0.00757354847065983 1119 18-19 0.060119057655413144 1119 20-21 0.04208563870361104 1119 22-23 0.025580658985560945 1119 24-25 0.008117744958589412 1119 26-27 0.06675275830078675 1119 28-29 -0.01640319712522853 1119 30-31 0.10517379283737682 1119 32-33 0.08986429778490646 1119 34-35 0.05691004323089999 1119 36-37 0.06077449272103763 1119 38-39 0.12751717715163835 1119 40-41 1.1526707290963856 1119 42-43 0.3946273464988863 1119 44-45 0.07854065269012978 1119 46-47 0.5316340295783846 1119 48-49 0.19810894050747407 1119 50-51 0.06983831835385246 1119 52-53 0.10318777884590702 1119 54-55 0.1365093528936967 1119 56-57 0.08789901081909335 1119 58-59 0.1536776428293507 1119 60-61 0.15564564348454013 1119 62-63 0.07406122792662018 1119 64-65 0.1160849798359962 1119 66-67 0.05644012583173463 1119 68-69 0.15496878945332782 1119 70-71 0.1804187976746192 1119 72-73 0.10492857889343554 1119 74-75 0.103526202118843 1119 76 0.17573646145065425 2119 1 -0.05365478044905103 2119 2 0.030910680431944115 2119 3 0.01764226256922541 2119 4 0.00231208834569685 2119 5 0.04285573884877891 2119 6 -0.006004673818516437 2119 7 0.022135650073273894 2119 8 0.08104350223018031 2119 9 0.11105369901638795 2119 10-11 0.021762873456914633 2119 12-13 0.06525198441672586 2119 14-15 0.06855711394410946 2119 16-17 0.01915809992885187 2119 18-19 0.06817982676889045 2119 20-21 0.018146991500138654 2119 22-23 0.10497498624717849 2119 24-25 0.07025630151461115 2119 26-27 0.07921066011405031 2119 28-29 0.06535235018964869 2119 30-31 0.10730659041271906 2119 32-33 0.046840126585387054 2119 34-35 0.11346359425105135 2119 36-37 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2104 48-49 -0.09169680579505268 2104 50-51 -0.15642515648350042 2104 52-53 -0.14125795713993483 2104 54-55 -0.29686217875503473 2104 56-57 -0.07103543230054044 2104 58-59 -0.1936599130616088 2104 60-61 -0.24693901460825174 2104 62-63 -0.10355976455250016 2104 64-65 -0.10056458369150079 2104 66-67 -0.22644579603431225 2104 68-69 -0.29772204899773413 2104 70-71 -0.1595582441985286 2104 72-73 -0.12888865195178312 2104 74-75 -0.0743162603574703 2104 76 -0.24119932526341614 2111 1 -0.09775875855268623 2111 2 -0.03217955089627367 2111 3 5.107020951840013E-4 2111 4 0.002575625343332888 2111 5 -0.013402787634639424 2111 6 0.03385631966727232 2111 7 0.006341447341839057 2111 8 0.010069268474225623 2111 9 -0.03672851930677723 2111 10-11 -0.02697067836735556 2111 12-13 0.006240246210062139 2111 14-15 0.04395799014132251 2111 16-17 0.007278093602401725 2111 18-19 -0.04341421367205811 2111 20-21 -0.013988404187124104 2111 22-23 -0.03635369210990547 2111 24-25 -0.019453990880954564 2111 26-27 0.07393996885631537 2111 28-29 -0.02475475724540388 2111 30-31 -0.014090471217926392 2111 32-33 -0.009215877656131966 2111 34-35 0.009242356791943962 2111 36-37 0.04320400550336956 2111 38-39 -0.03019294696833441 2111 40-41 1.1181105764475276 2111 42-43 0.030485717986707073 2111 44-45 -0.11585614697217039 2111 46-47 0.4542283643141971 2111 48-49 0.07814440446134086 2111 50-51 -0.1947602168541991 2111 52-53 -0.18097014746761886 2111 54-55 -0.12810019956620522 2111 56-57 -0.231694069882856 2111 58-59 -0.10308414717641767 2111 60-61 -0.21898605171873697 2111 62-63 -0.2646169221956143 2111 64-65 -0.1428225649327004 2111 66-67 -0.03337183480613248 2111 68-69 0.014721492739020903 2111 70-71 -0.04525530351092044 2111 72-73 -0.31340062973542615 2111 74-75 -0.47016732576756226 2111 76 -0.12424339823981256 2110 1 0.016709995798407817 2110 2 0.00980540537121044 2110 3 -0.02388710558300744 2110 4 -0.01873606535195904 2110 5 -0.0563528971241567 2110 6 0.041118702108441596 2110 7 0.04348165087841238 2110 8 -0.018237409753183442 2110 9 -0.06614512373952408 2110 10-11 -0.04304918171533245 2110 12-13 -0.11295617671058977 2110 14-15 -0.05440536314905842 2110 16-17 -0.09214851045060612 2110 18-19 -0.06675312168820824 2110 20-21 -0.1712514195156558 2110 22-23 -0.06441987314099862 2110 24-25 -0.11338159489599064 2110 26-27 0.004019760130063332 2110 28-29 -0.1189426361867092 2110 30-31 -0.01759310719377538 2110 32-33 -0.08595465656326695 2110 34-35 -0.007788642030888582 2110 36-37 -0.07942148023768425 2110 38-39 -0.03279530067642611 2110 40-41 0.9855337280999947 2110 42-43 -0.03363447365158123 2110 44-45 -0.27171257014597217 2110 46-47 -0.006790521044585773 2110 48-49 -0.2062395746280572 2110 50-51 -0.3387136110290996 2110 52-53 -0.36144074668536774 2110 54-55 -0.3047426213729736 2110 56-57 -0.34541483048688804 2110 58-59 -0.37003919951619935 2110 60-61 -0.2083559174200431 2110 62-63 -0.3083442964725407 2110 64-65 -0.26773000515648704 2110 66-67 -0.5825167248735994 2110 68-69 -0.5456397435431413 2110 70-71 -0.303980446859903 2110 72-73 -0.5740509373186882 2110 74-75 -0.346164123357525 2110 76 -0.38143378974393016 2109 1 0.03538893750474159 2109 2 0.02599399923648349 2109 3 -0.048271642224086975 2109 4 -0.047498333941049964 2109 5 0.007590384989022425 2109 6 0.04810565668642397 2109 7 -0.045714822687152434 2109 8 -0.01891699046087325 2109 9 -0.0678237858333901 2109 10-11 -0.06184452988023281 2109 12-13 -0.08455695014709619 2109 14-15 -0.022292582963949314 2109 16-17 -0.16463606611639392 2109 18-19 -0.13275543559979752 2109 20-21 -0.22041433958598589 2109 22-23 -0.19235076858016242 2109 24-25 -0.1375114985797694 2109 26-27 -0.12407676027453363 2109 28-29 -0.17521730961673398 2109 30-31 -0.18802405989819704 2109 32-33 -0.2652942083776608 2109 34-35 -0.09963195558071902 2109 36-37 -0.3560885806722638 2109 38-39 -0.042470118888829234 2109 40-41 -10.873602855873003 2109 42-43 -0.7243395512242969 2109 44-45 -0.16897365156690825 2109 46-47 0.2543925951020043 2109 48-49 -0.02793045199508981 2109 50-51 -0.040690664640443686 2109 52-53 -0.0054196892605560265 2109 54-55 -0.27499376114919016 2109 56-57 -0.07898794401112497 2109 58-59 -0.11009548139715264 2109 60-61 -0.20948700511251417 2109 62-63 -0.029004685418215104 2109 64-65 -0.15832580480863356 2109 66-67 -0.14883814453082778 2109 68-69 -0.4945307424705021 2109 70-71 -0.20616792069559864 2109 72-73 -0.12342707176330237 2109 74-75 -0.3796049639957957 2109 76 -0.020442354257795614 2108 1 0.0924013547812308 2108 2 -0.012964897235775652 2108 3 -0.0011616701691323783 2108 4 0.05993714303230746 2108 5 0.028232271317797597 2108 6 0.059580541550182886 2108 7 -0.04551262875656903 2108 8 -0.017410802456723218 2108 9 -0.04263875664019423 2108 10-11 -0.05563907778512345 2108 12-13 -0.16591805819005856 2108 14-15 -0.13425764903148973 2108 16-17 -0.30920143918695686 2108 18-19 -0.33246047926114386 2108 20-21 -0.3385005512748407 2108 22-23 -0.0932767540707502 2108 24-25 -0.37496986119531783 2108 26-27 -0.33538418527262337 2108 28-29 -0.6985479504025776 2108 30-31 -0.25748000144625394 2108 32-33 -0.5940426754867119 2108 34-35 -0.21656943466845746 2108 36-37 -0.6724536051479504 2108 38-39 -0.26766717452443345 2108 40-41 -7.404634010528802 2108 42-43 -0.38655132625436295 2108 44-45 -0.08083419176759321 2108 46-47 0.48849963630586757 2108 48-49 0.22890631246506388 2108 50-51 0.03937899162379921 2108 52-53 -0.14702346238743047 2108 54-55 -0.22570299757832402 2108 56-57 -0.0705008057298997 2108 58-59 -0.1287850685007612 2108 60-61 -0.26159993674446724 2108 62-63 -0.12918009548045006 2108 64-65 -0.07911762213456086 2108 66-67 -0.06472405334348963 2108 68-69 0.04411869310600025 2108 70-71 0.10166788812260563 2108 72-73 -0.06927581492421808 2108 74-75 -0.079167541348383 2108 76 -0.4673576982180947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 5.0 15 17.0 16 23.0 17 62.0 18 95.0 19 166.0 20 182.0 21 229.0 22 306.0 23 364.0 24 604.0 25 890.0 26 1291.0 27 1946.0 28 2895.0 29 4121.0 30 5755.0 31 8526.0 32 12814.0 33 19073.0 34 29078.0 35 47637.0 36 106595.0 37 967891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75712703220887 26.19466745748067 7.893986867310939 24.15421864299952 2 15.225262211839578 22.781390868906882 41.366566245404016 20.626780673849527 3 19.00828289553573 25.29599766060036 32.26504604866975 23.43067339519415 4 13.648067393213262 16.11459753983051 41.365657580290886 28.871677486665337 5 12.145713537540672 36.650263884609444 38.05249936599957 13.151523211850316 6 27.80044392421072 39.33685620044161 17.91094586255862 14.951754012789047 7 23.846511593327754 34.90579207924881 22.970723636114318 18.276972691309116 8 20.671966111747636 42.1108455789725 21.887099185753453 15.330089123526413 9 21.125803032793726 17.528810879530006 25.190096871961654 36.15528921571462 10-11 23.79137214214496 26.995986178377567 30.263050289657656 18.949591389819812 12-13 20.679731068168884 25.003944432650155 31.471492284194102 22.844832214986862 14-15 19.9737395782307 28.967169929462806 25.43242133644813 25.626669155858373 16-17 18.990357411031358 34.48541055554959 27.699540793694194 18.824691239724856 18-19 18.620985042546177 32.526452480531844 30.636016015635647 18.216546461286324 20-21 18.761497711403003 30.263339410375465 31.423002609520996 19.552160268700536 22-23 19.152884557401613 30.678764578912194 31.049376036188004 19.11897482749819 24-25 19.15478449354724 31.144744570106404 30.84885016690526 18.851620769441098 26-27 18.840097243688287 31.359932990078203 30.803416911249027 18.996552854984483 28-29 18.624041461563053 31.29793724758729 31.129958110538286 18.948063180311376 30-31 18.777275442003624 30.98750420257615 31.19244948854545 19.04277086687478 32-33 18.84872956226297 31.156309398818898 30.91158936267055 19.083371676247577 34-35 18.82072615559486 31.062592983287995 31.199264476893884 18.91741638422326 36-37 18.892923729128583 31.298391580143846 31.221898498802624 18.586786191924944 38-39 18.737541994784262 31.12334963698829 31.06255168032831 19.076556687899142 40-41 18.746752554794572 31.088572544931424 31.111371778678915 19.053303121595086 42-43 18.935589686485756 31.16717207721671 31.032730943433943 18.864507292863593 44-45 18.620530709989616 30.948720723429602 31.36410458900664 19.066643977574145 46-47 18.714784063996458 30.86471050342525 31.556617684110005 18.86388774846828 48-49 18.61999377151368 31.090183360359237 31.388968970738507 18.90085389738858 50-51 18.496126195410913 31.067714550289242 31.503997713468152 18.932161540831693 52-53 18.383575630262513 30.803127790531214 31.775977703010245 19.03731887619603 54-55 18.41137252213219 30.72717164766593 31.746735207551502 19.114720622650378 56-57 18.25268655101287 30.718456723171865 31.833595331774283 19.195261394040976 58-59 18.09705699891043 30.902213590821493 31.847431823269588 19.153297586998487 60-61 18.292956936708173 30.705198473112187 31.730585750313693 19.271258839865947 62-63 18.029154933184202 30.321411371696072 32.00124404514579 19.648189649973936 64-65 18.050302048544197 30.097962359786777 32.014378386326406 19.83735720534262 66-67 18.089663769126368 30.16408839824644 31.67701581159903 20.069232021028164 68-69 17.922469388311427 30.341402004184815 31.564919579007196 20.171209028496566 70-71 17.966539646297974 30.31852016451795 31.51531472442252 20.199625464761553 72-73 17.741645030799617 30.19585037424612 31.542079042300013 20.520425552654252 74-75 17.99636038319234 30.04567281282242 31.249901905470743 20.708064898514497 76 17.952579245923605 29.989665999486192 31.110545719485167 20.94720903510504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 5.5 2 6.5 3 7.0 4 4.0 5 5.5 6 7.0 7 14.5 8 22.0 9 36.0 10 87.0 11 231.0 12 338.0 13 501.0 14 837.0 15 1355.0 16 2286.0 17 2872.0 18 3894.5 19 6036.5 20 8936.0 21 10716.0 22 12598.5 23 17160.5 24 22639.0 25 25438.0 26 28420.5 27 34284.5 28 39718.0 29 42270.0 30 45076.5 31 50048.5 32 53682.5 33 55151.0 34 56667.5 35 59769.0 36 62228.0 37 63102.0 38 63563.5 39 64632.0 40 64733.5 41 63410.5 42 62593.0 43 61340.5 44 58467.0 45 54695.0 46 52544.0 47 49491.0 48 43109.0 49 37032.5 50 34285.0 51 31660.0 52 26033.0 53 20775.5 54 18520.0 55 16488.5 56 12800.0 57 9715.5 58 8288.0 59 7450.5 60 5747.0 61 4112.0 62 3343.0 63 2887.5 64 1995.0 65 1316.0 66 907.0 67 740.0 68 611.5 69 397.0 70 248.5 71 186.0 72 154.5 73 102.0 74 61.5 75 42.0 76 33.5 77 20.0 78 13.0 79 11.0 80 8.0 81 3.0 82 1.0 83 1.0 84 1.5 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1210567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.62488349438634 #Duplication Level Percentage of deduplicated Percentage of total 1 94.75085100766984 85.86784833564037 2 4.028348628071206 7.301372501874481 3 0.5721189759660147 1.555446466255431 4 0.1944258826009045 0.7047929183600082 5 0.09753547454573286 0.4419570508638362 6 0.0629091749609169 0.3420681990936636 7 0.04408201395587325 0.2796449165264248 8 0.04573554469235856 0.3315822727437835 9 0.034513004710437625 0.2814963327832155 >10 0.16561177543026365 2.59210717364286 >50 0.0027555338362006774 0.17098086908537194 >100 0.001112983560176835 0.13070296313052532 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.478177581249117E-4 0.0 0.0 2 2.478177581249117E-4 0.0 0.0 3 2.478177581249117E-4 0.0 0.0 4 2.478177581249117E-4 0.0 8.260591937497058E-5 5 2.478177581249117E-4 0.0 8.260591937497058E-5 6 2.478177581249117E-4 0.0 8.260591937497058E-5 7 2.478177581249117E-4 0.0 8.260591937497058E-5 8 2.478177581249117E-4 0.0 8.260591937497058E-5 9 2.478177581249117E-4 0.0 8.260591937497058E-5 10 4.1302959687485285E-4 0.0 8.260591937497058E-5 11 4.1302959687485285E-4 0.0 8.260591937497058E-5 12 4.1302959687485285E-4 0.0 8.260591937497058E-5 13 4.1302959687485285E-4 1.6521183874994115E-4 8.260591937497058E-5 14 4.1302959687485285E-4 2.478177581249117E-4 8.260591937497058E-5 15 4.1302959687485285E-4 2.478177581249117E-4 8.260591937497058E-5 16 4.1302959687485285E-4 2.478177581249117E-4 8.260591937497058E-5 17 4.956355162498234E-4 2.478177581249117E-4 8.260591937497058E-5 18 4.956355162498234E-4 2.478177581249117E-4 8.260591937497058E-5 19 4.956355162498234E-4 3.304236774998823E-4 8.260591937497058E-5 20 4.956355162498234E-4 3.304236774998823E-4 8.260591937497058E-5 21 4.956355162498234E-4 3.304236774998823E-4 2.478177581249117E-4 22 4.956355162498234E-4 3.304236774998823E-4 6.608473549997646E-4 23 4.956355162498234E-4 3.304236774998823E-4 8.260591937497057E-4 24 5.78241435624794E-4 3.304236774998823E-4 9.912710324996468E-4 25 5.78241435624794E-4 3.304236774998823E-4 0.0012390887906245586 26 6.608473549997646E-4 3.304236774998823E-4 0.0015695124681244408 27 6.608473549997646E-4 3.304236774998823E-4 0.004047690049373558 28 7.434532743747351E-4 3.304236774998823E-4 0.010903981357496116 29 7.434532743747351E-4 3.304236774998823E-4 0.016521183874994114 30 7.434532743747351E-4 3.304236774998823E-4 0.023542687021866612 31 7.434532743747351E-4 3.304236774998823E-4 0.02849904218436485 32 8.260591937497057E-4 3.304236774998823E-4 0.03485969797623758 33 8.260591937497057E-4 3.304236774998823E-4 0.04163338336498517 34 8.260591937497057E-4 3.304236774998823E-4 0.053280817996856016 35 8.260591937497057E-4 3.304236774998823E-4 0.06930636635560031 36 8.260591937497057E-4 3.304236774998823E-4 0.10144006899246386 37 9.912710324996468E-4 3.304236774998823E-4 0.15827294152244362 38 9.912710324996468E-4 4.1302959687485285E-4 0.2242750711030451 39 9.912710324996468E-4 4.1302959687485285E-4 0.29961166957301827 40 9.912710324996468E-4 4.1302959687485285E-4 0.3814741356736141 41 9.912710324996468E-4 4.1302959687485285E-4 0.47457100680920594 42 9.912710324996468E-4 4.956355162498234E-4 0.5730372627041709 43 9.912710324996468E-4 4.956355162498234E-4 0.6870334314416302 44 0.0010738769518746174 4.956355162498234E-4 0.8089597684390868 45 0.0010738769518746174 4.956355162498234E-4 0.9619459311215323 46 0.001156482871249588 4.956355162498234E-4 1.1433485300689676 47 0.001156482871249588 4.956355162498234E-4 1.344411337827646 48 0.001156482871249588 4.956355162498234E-4 1.5486131705225734 49 0.0012390887906245586 4.956355162498234E-4 1.7816444690793654 50 0.0013216947099995292 4.956355162498234E-4 2.026736231864903 51 0.0013216947099995292 4.956355162498234E-4 2.2608414073735696 52 0.0013216947099995292 4.956355162498234E-4 2.522949989550351 53 0.0013216947099995292 4.956355162498234E-4 2.784232512533383 54 0.0013216947099995292 4.956355162498234E-4 3.0545190807282867 55 0.0014043006293744998 4.956355162498234E-4 3.3362052657969365 56 0.0014869065487494702 4.956355162498234E-4 3.651925089648074 57 0.0015695124681244408 4.956355162498234E-4 3.9901137235692037 58 0.0015695124681244408 4.956355162498234E-4 4.359775212772197 59 0.0015695124681244408 4.956355162498234E-4 4.738192929428937 60 0.0015695124681244408 4.956355162498234E-4 5.149818225674415 61 0.0015695124681244408 4.956355162498234E-4 5.547070091948648 62 0.0016521183874994114 4.956355162498234E-4 5.970012399148498 63 0.0016521183874994114 4.956355162498234E-4 6.392376464912723 64 0.0016521183874994114 4.956355162498234E-4 6.819614279920071 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAG 220 0.0 23.863636 1 GTGTTAC 290 0.0 22.931034 1 CTAATAC 270 0.0 20.74074 3 GTGTAAG 300 0.0 19.833334 1 GTATAGG 265 3.6379788E-12 19.811321 1 TAGCGTG 125 7.8267985E-5 19.6 5 GCGCTAG 90 0.005277023 19.444445 1 GTGTATG 490 0.0 19.285715 1 GTGTACA 490 0.0 19.285715 1 TACTCCG 190 3.0073534E-7 18.421051 5 TACGCCT 210 4.2726242E-8 18.333332 4 CTACGCC 210 4.2726242E-8 18.333332 3 CTGTGCG 210 4.2726242E-8 18.333332 9 GGTTAAG 250 8.6583896E-10 18.2 1 GTAATAC 385 0.0 18.181818 3 GTATACG 200 5.151069E-7 17.500002 1 CGGGGAT 120 0.0013411242 17.5 37 GTCCATG 240 1.029548E-8 17.5 1 TAAGGCA 225 9.400537E-8 17.11111 4 ACCGATC 205 6.671107E-7 17.073172 8 >>END_MODULE