FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve15.3410000000c846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve15.3410000000c846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210567
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG2200.023.8636361
GTGTTAC2900.022.9310341
CTAATAC2700.020.740743
GTGTAAG3000.019.8333341
GTATAGG2653.6379788E-1219.8113211
TAGCGTG1257.8267985E-519.65
GCGCTAG900.00527702319.4444451
GTGTATG4900.019.2857151
GTGTACA4900.019.2857151
TACTCCG1903.0073534E-718.4210515
TACGCCT2104.2726242E-818.3333324
CTACGCC2104.2726242E-818.3333323
CTGTGCG2104.2726242E-818.3333329
GGTTAAG2508.6583896E-1018.21
GTAATAC3850.018.1818183
GTATACG2005.151069E-717.5000021
CGGGGAT1200.001341124217.537
GTCCATG2401.029548E-817.51
TAAGGCA2259.400537E-817.111114
ACCGATC2056.671107E-717.0731728
Produced by FastQC (version 0.11.2)