##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve14.3410000000c82c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1362122 Sequences flagged as poor quality 0 Sequence length 76 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74216333045057 34.0 34.0 34.0 34.0 34.0 2 33.765901292248415 34.0 34.0 34.0 34.0 34.0 3 33.78922519421902 34.0 34.0 34.0 34.0 34.0 4 33.81035472593498 34.0 34.0 34.0 34.0 34.0 5 33.81709127376256 34.0 34.0 34.0 34.0 34.0 6 37.6004579619153 38.0 38.0 38.0 37.0 38.0 7 37.62568330883724 38.0 38.0 38.0 37.0 38.0 8 37.53184149437422 38.0 38.0 38.0 37.0 38.0 9 37.50479545885023 38.0 38.0 38.0 37.0 38.0 10-11 37.504167027623076 38.0 38.0 38.0 37.0 38.0 12-13 37.43728204962551 38.0 38.0 38.0 37.0 38.0 14-15 37.49334677804191 38.0 38.0 38.0 37.0 38.0 16-17 37.43767885695995 38.0 38.0 38.0 37.0 38.0 18-19 37.456112961981376 38.0 38.0 38.0 37.0 38.0 20-21 37.402502859508914 38.0 38.0 38.0 37.0 38.0 22-23 37.46041690832392 38.0 38.0 38.0 37.0 38.0 24-25 37.38988871775069 38.0 38.0 38.0 37.0 38.0 26-27 37.348890921664875 38.0 38.0 38.0 37.0 38.0 28-29 37.27195104403277 38.0 38.0 38.0 37.0 38.0 30-31 37.297330195092655 38.0 38.0 38.0 37.0 38.0 32-33 37.24388307361602 38.0 38.0 38.0 37.0 38.0 34-35 37.2949339339648 38.0 38.0 38.0 37.0 38.0 36-37 37.19026966747472 38.0 38.0 38.0 37.0 38.0 38-39 37.25682024077139 38.0 38.0 38.0 37.0 38.0 40-41 36.06822002728096 38.0 37.5 38.0 32.5 38.0 42-43 36.75732680332599 38.0 37.0 38.0 35.0 38.0 44-45 36.96449069907101 38.0 38.0 38.0 36.0 38.0 46-47 36.527367959698175 38.0 37.5 38.0 35.0 38.0 48-49 36.86792078829943 38.0 37.5 38.0 35.0 38.0 50-51 36.93181411063033 38.0 38.0 38.0 36.0 38.0 52-53 36.950955567856624 38.0 38.0 38.0 36.0 38.0 54-55 36.93301921560624 38.0 38.0 38.0 36.0 38.0 56-57 36.93529544343311 38.0 38.0 38.0 36.0 38.0 58-59 36.90183148058691 38.0 38.0 38.0 36.0 38.0 60-61 36.90432978837431 38.0 38.0 38.0 36.0 38.0 62-63 36.91163346601846 38.0 38.0 38.0 36.0 38.0 64-65 36.82925905315383 38.0 38.0 38.0 35.5 38.0 66-67 36.80208013672784 38.0 38.0 38.0 35.0 38.0 68-69 36.79059841923117 38.0 38.0 38.0 35.0 38.0 70-71 36.70806065829639 38.0 37.5 38.0 34.5 38.0 72-73 36.75753640275981 38.0 38.0 38.0 35.0 38.0 74-75 36.75470479149445 38.0 38.0 38.0 35.0 38.0 76 36.02029553887244 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.06377698993583891 1101 2 0.09264634812281258 1101 3 0.09444932505103054 1101 4 0.07530244011235965 1101 5 0.07088071259582307 1101 6 0.14487464481226198 1101 7 0.0899751699567588 1101 8 0.17177304516318515 1101 9 0.120172616307201 1101 10-11 0.1310805282934453 1101 12-13 0.1822825607369083 1101 14-15 0.09900511728431383 1101 16-17 0.14105460178591755 1101 18-19 0.15740240062063293 1101 20-21 0.20986898653723784 1101 22-23 0.13797398278209982 1101 24-25 0.1717389109024836 1101 26-27 0.129269364928831 1101 28-29 0.22414421712348087 1101 30-31 0.21919463034937792 1101 32-33 0.2899780519294737 1101 34-35 0.18085432085654674 1101 36-37 0.2007895021131958 1101 38-39 0.16171679176400033 1101 40-41 1.4189348771733066 1101 42-43 0.6408430088149757 1101 44-45 0.38181662101357716 1101 46-47 0.8647507074862517 1101 48-49 0.5312920484315455 1101 50-51 0.2666799356195071 1101 52-53 0.29767443865982557 1101 54-55 0.29244927736808535 1101 56-57 0.2757778514907159 1101 58-59 0.3022472178115194 1101 60-61 0.31080772659327494 1101 62-63 0.27035220245059577 1101 64-65 0.2481063791710767 1101 66-67 0.29239702720650484 1101 68-69 0.38934632861364094 1101 70-71 0.38122568833745873 1101 72-73 0.33223276691574455 1101 74-75 0.35505374381696697 1101 76 0.4811691544162713 1102 1 -0.02281553583816276 1102 2 0.05219929705965143 1102 3 0.04561892208290175 1102 4 0.05950666362540602 1102 5 0.003918495041659753 1102 6 -0.01507061480744909 1102 7 0.07610057828135552 1102 8 0.11141379927920525 1102 9 0.024090486315444082 1102 10-11 0.08706092524443676 1102 12-13 0.10174685134367678 1102 14-15 0.016502652337194945 1102 16-17 0.11177057918643385 1102 18-19 0.11288148472501547 1102 20-21 0.10711445365294736 1102 22-23 0.04404141152752317 1102 24-25 0.08128983100490217 1102 26-27 0.10211582655490758 1102 28-29 0.15511921251958682 1102 30-31 0.10138625011875035 1102 32-33 0.2135389233780174 1102 34-35 0.15618362495940374 1102 36-37 0.014368230772717538 1102 38-39 0.10710240729329001 1102 40-41 1.2704413300805868 1102 42-43 0.6732434944867265 1102 44-45 0.37020853217213556 1102 46-47 0.8336890791264153 1102 48-49 0.42410338521146684 1102 50-51 0.23860395386306266 1102 52-53 0.2989460496479879 1102 54-55 0.3020753857272709 1102 56-57 0.24416112016145775 1102 58-59 0.3361862189105551 1102 60-61 0.31047017709925484 1102 62-63 0.2617592736841843 1102 64-65 0.2481803882762037 1102 66-67 0.3418638252445305 1102 68-69 0.2012487891909629 1102 70-71 0.2959919960911819 1102 72-73 0.3232142337734345 1102 74-75 0.3251832666871337 1102 76 0.42921261239634134 1103 1 -0.013757543062048683 1103 2 -0.008700904854279656 1103 3 -0.023212423828063322 1103 4 0.040553813195352006 1103 5 0.04342276025666081 1103 6 0.09720422530274675 1103 7 0.07301682990505753 1103 8 0.06527755728370721 1103 9 0.15872316951021048 1103 10-11 0.1212666268140481 1103 12-13 0.16220020548729508 1103 14-15 0.07523468367934072 1103 16-17 0.1901971397448463 1103 18-19 0.1693616098339632 1103 20-21 0.195093693671744 1103 22-23 0.16772337393175007 1103 24-25 0.2024456739592253 1103 26-27 0.18232918108628837 1103 28-29 0.20708080819815677 1103 30-31 0.23699397608227457 1103 32-33 0.26285971968447797 1103 34-35 0.18845831333383245 1103 36-37 0.1475420729603627 1103 38-39 0.13688269320702062 1103 40-41 1.4216162320301322 1103 42-43 0.6398572011574757 1103 44-45 0.40006278926997396 1103 46-47 0.8258692782697636 1103 48-49 0.4096754567832619 1103 50-51 0.2771564635339345 1103 52-53 0.277832650558274 1103 54-55 0.25210324961157227 1103 56-57 0.26422943918842634 1103 58-59 0.3419822791617406 1103 60-61 0.35237362355932333 1103 62-63 0.33831179220631213 1103 64-65 0.35266886559589494 1103 66-67 0.3101742679034203 1103 68-69 0.3410086308181661 1103 70-71 0.33468552869852175 1103 72-73 0.4144756026281087 1103 74-75 0.3832577061446685 1103 76 0.5848183391006998 2116 1 0.0275144852371767 2116 2 -0.040368351341932396 2116 3 0.04250968629073526 2116 4 0.04407550962751827 2116 5 0.057174773522469025 2116 6 -0.010868397430662924 2116 7 0.12293955332024353 2116 8 0.10491953317234248 2116 9 0.17737301730601018 2116 10-11 0.16100664673135867 2116 12-13 0.18572436288091865 2116 14-15 0.11707127513360405 2116 16-17 0.19270705279205202 2116 18-19 0.20250471756060762 2116 20-21 0.19206070305347112 2116 22-23 0.1251618412669302 2116 24-25 0.20290620620166777 2116 26-27 0.20055138928432825 2116 28-29 0.20759008071920704 2116 30-31 0.20694312912485202 2116 32-33 0.24951886447713179 2116 34-35 0.1420145329561251 2116 36-37 0.186895199307628 2116 38-39 0.1789620704133199 2116 40-41 1.4183610928009145 2116 42-43 0.5801606432001805 2116 44-45 0.4226912031760861 2116 46-47 0.8533057938807715 2116 48-49 0.4451154149229666 2116 50-51 0.34026293927863804 2116 52-53 0.35171108004789886 2116 54-55 0.33469435607435827 2116 56-57 0.3218765106900605 2116 58-59 0.3796563663010488 2116 60-61 0.42717030017666247 2116 62-63 0.3398890543873847 2116 64-65 0.45082685636383246 2116 66-67 0.4389498874525799 2116 68-69 0.4311688842056327 2116 70-71 0.46154270411626896 2116 72-73 0.4090147897064682 2116 74-75 0.3660245663895978 2116 76 0.5341519742668979 1117 1 0.050191018778633634 1117 2 -6.635871721840658E-4 1117 3 0.05762444439766057 1117 4 0.06277520246702295 1117 5 0.05047001326062173 1117 6 0.008725784496334654 1117 7 0.09163292374507392 1117 8 0.08189442941775127 1117 9 0.2130207591572173 1117 10-11 0.15093691035790613 1117 12-13 0.13784777306578633 1117 14-15 0.10602938027383146 1117 16-17 0.1774067394905785 1117 18-19 0.168075087292074 1117 20-21 0.1820080288255852 1117 22-23 0.16336729992531218 1117 24-25 0.19841101123682137 1117 26-27 0.24363840106272505 1117 28-29 0.24853001861718838 1117 30-31 0.23583513362898145 1117 32-33 0.2029006166206102 1117 34-35 0.20751893603643623 1117 36-37 0.28689095645098206 1117 38-39 0.20320807710363198 1117 40-41 1.3860144748601115 1117 42-43 0.550017763606867 1117 44-45 0.3331569488078969 1117 46-47 0.7500684384309935 1117 48-49 0.4076552475311104 1117 50-51 0.2645794834607358 1117 52-53 0.23309169443586342 1117 54-55 0.3406618502097274 1117 56-57 0.2297871913000904 1117 58-59 0.226697266880862 1117 60-61 0.33259493196928247 1117 62-63 0.24790755100928408 1117 64-65 0.3500421232785911 1117 66-67 0.28228669741614 1117 68-69 0.3120187228095972 1117 70-71 0.3124648764477129 1117 72-73 0.28267570786064766 1117 74-75 0.3476643369738781 1117 76 0.6218893648877284 2117 1 0.06685366401416815 2117 2 0.0802211726724451 2117 3 0.07770390000275285 2117 4 0.09549854375323008 2117 5 0.07226581848611602 2117 6 0.07513058006702522 2117 7 0.13091717368348554 2117 8 0.13528504424113663 2117 9 0.17684116654225335 2117 10-11 0.05868858445437297 2117 12-13 -0.00506896812390778 2117 14-15 0.05840762090051754 2117 16-17 0.20024403913613043 2117 18-19 0.2162159417140188 2117 20-21 0.2048337633413908 2117 22-23 0.20392026920383444 2117 24-25 0.20670584569446504 2117 26-27 0.2531567137602053 2117 28-29 0.3086228546204097 2117 30-31 0.30541661372936346 2117 32-33 0.25618865682817216 2117 34-35 0.24494102432344533 2117 36-37 0.34828499258424017 2117 38-39 0.20028266171510722 2117 40-41 1.430387311583253 2117 42-43 0.7245929050318765 2117 44-45 0.4752902842784934 2117 46-47 0.9554699234920747 2117 48-49 0.5458141154335578 2117 50-51 0.3793246583058121 2117 52-53 0.3694916080454007 2117 54-55 0.46406283920057234 2117 56-57 0.4488821415074611 2117 58-59 0.4841840889603759 2117 60-61 0.5135192593216473 2117 62-63 0.4156273826828638 2117 64-65 -0.014271275005064865 2117 66-67 0.4224490746500109 2117 68-69 0.41999007109703257 2117 70-71 0.5968294467395125 2117 72-73 0.48725399471973674 2117 74-75 0.4919892469556828 2117 76 0.7201617524479502 1116 1 0.04327834278837628 1116 2 0.018792950231087957 1116 3 0.07233330850855424 1116 4 0.01222649493341521 1116 5 0.0507563482730049 1116 6 0.01803931777403278 1116 7 0.03622908874107367 1116 8 -0.012030785825743351 1116 9 0.12080894581664126 1116 10-11 0.07769610072733002 1116 12-13 -0.007090527708719208 1116 14-15 0.1363222421325787 1116 16-17 0.12604929788030006 1116 18-19 0.18935049088410238 1116 20-21 0.13385902653657666 1116 22-23 0.09952590312992271 1116 24-25 0.09360276143706159 1116 26-27 0.16086234227250884 1116 28-29 0.29230345745250474 1116 30-31 0.17861138512454033 1116 32-33 0.1379132513886745 1116 34-35 0.19016456263045 1116 36-37 0.3004088177232376 1116 38-39 0.25543950030803586 1116 40-41 1.3853437764624132 1116 42-43 0.5698778301991325 1116 44-45 0.38622611662241724 1116 46-47 0.7821555546540964 1116 48-49 0.41165576867339837 1116 50-51 0.28334929859765623 1116 52-53 0.28421977478897276 1116 54-55 0.3137832244167029 1116 56-57 0.2943395032615683 1116 58-59 0.28660412316959594 1116 60-61 0.2785431763397739 1116 62-63 0.2489456463431381 1116 64-65 0.01451907069181857 1116 66-67 0.20409995839281692 1116 68-69 0.2598512903486352 1116 70-71 0.3168125814107938 1116 72-73 0.381167202014133 1116 74-75 0.326641335495907 1116 76 0.7314310445410612 2118 1 0.014481295335194488 2118 2 -0.012095808417434739 2118 3 0.01850214235874148 2118 4 0.01924906480257249 2118 5 0.015330228046799732 2118 6 0.04487998372102453 2118 7 0.06922989273822111 2118 8 0.04200587325204452 2118 9 0.14396933821722513 2118 10-11 0.09389020652946556 2118 12-13 0.06887328860238995 2118 14-15 0.11909060871364119 2118 16-17 0.16093618134512866 2118 18-19 0.16352785119870106 2118 20-21 0.12165419707107361 2118 22-23 0.16654713358215645 2118 24-25 0.12835941610102708 2118 26-27 0.15580684148499557 2118 28-29 0.19400590494198866 2118 30-31 0.17828308917863467 2118 32-33 0.15204028293888427 2118 34-35 0.1843401116092096 2118 36-37 0.22455264056488033 2118 38-39 0.21057555696051367 2118 40-41 1.3374214515399885 2118 42-43 0.5198951975092356 2118 44-45 0.27344228428687956 2118 46-47 0.755329427973713 2118 48-49 0.26170453941136884 2118 50-51 0.16918319493719736 2118 52-53 0.2307317561509521 2118 54-55 0.21337435525587267 2118 56-57 0.20801831594133802 2118 58-59 0.1253312815133114 2118 60-61 0.23282263075986265 2118 62-63 0.2667248445043242 2118 64-65 0.229941172667246 2118 66-67 0.2054000256065578 2118 68-69 0.2002902304603893 2118 70-71 0.24571626171903915 2118 72-73 0.20042923186925066 2118 74-75 0.21714077491868977 2118 76 0.544580579336575 1119 1 0.029283354894410252 1119 2 -0.007628624758616809 1119 3 0.007748784208494897 1119 4 -0.010276528249427486 1119 5 0.023955204048874634 1119 6 -0.008694061230819727 1119 7 0.02292848432851713 1119 8 0.07400521066670507 1119 9 0.10174206728566304 1119 10-11 0.09688604873712592 1119 12-13 0.11476792782951861 1119 14-15 0.09345510550571845 1119 16-17 0.12056255529139293 1119 18-19 0.11671023613168074 1119 20-21 0.03453600667759105 1119 22-23 0.12760510078972231 1119 24-25 0.09430194028781358 1119 26-27 0.17222201219428257 1119 28-29 0.17825543347370143 1119 30-31 0.1928007429888794 1119 32-33 0.1591041635127297 1119 34-35 0.16375065323114057 1119 36-37 0.20352359562343736 1119 38-39 0.23666093898324192 1119 40-41 1.3252383021387786 1119 42-43 0.5809557984242488 1119 44-45 0.2595084818301885 1119 46-47 0.6806066083542532 1119 48-49 0.3644876057814912 1119 50-51 0.15046765405339357 1119 52-53 0.25867088701362917 1119 54-55 0.2258159891802478 1119 56-57 0.2198830986292819 1119 58-59 0.3147931942918518 1119 60-61 0.264173729604984 1119 62-63 0.2265204341532936 1119 64-65 0.23233181662951097 1119 66-67 0.228027696307187 1119 68-69 0.19078015548982563 1119 70-71 0.2847760489570632 1119 72-73 0.22723808639365473 1119 74-75 0.268886992256391 1119 76 0.3861655721943791 2119 1 -0.0034466534366757173 2119 2 0.05939093849183763 2119 3 -0.006028249502449512 2119 4 -0.034080331650514495 2119 5 0.07041478963644465 2119 6 0.002054754872304443 2119 7 0.07857181464966345 2119 8 0.07074027743085054 2119 9 0.11555210802313098 2119 10-11 0.04628110643780303 2119 12-13 0.05592999898785678 2119 14-15 0.09252017793772183 2119 16-17 -0.020426037735234104 2119 18-19 0.022831311288108225 2119 20-21 -0.007788705116531958 2119 22-23 0.0963593816098296 2119 24-25 0.08616376040858853 2119 26-27 0.011071827815342772 2119 28-29 0.1391828345896755 2119 30-31 0.1312112960010694 2119 32-33 0.17430021604234724 2119 34-35 0.16608129637376834 2119 36-37 0.2112835050623545 2119 38-39 0.10440295238070263 2119 40-41 1.3882921703143865 2119 42-43 0.49275687994975925 2119 44-45 0.22372025916224203 2119 46-47 0.7051963827207715 2119 48-49 0.3190310513155765 2119 50-51 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66-67 -0.39858150205362364 2104 68-69 -0.5827527569708408 2104 70-71 -0.4375969677702116 2104 72-73 -0.3163025789845051 2104 74-75 -0.3174459557709852 2104 76 -0.587254244387502 2111 1 -0.29104126399520425 2111 2 -0.11387652238042278 2111 3 -0.17515433711258765 2111 4 -0.07535336944148696 2111 5 -0.16842949673543472 2111 6 -0.06519413507234617 2111 7 -0.0546046503994404 2111 8 -0.18258941463706435 2111 9 -0.2445215904726652 2111 10-11 -0.19222333200615083 2111 12-13 -0.19817339614981933 2111 14-15 -0.13317494813582442 2111 16-17 -0.20545573651005355 2111 18-19 -0.1972598788692892 2111 20-21 -0.07971164953622889 2111 22-23 -0.18269013119976307 2111 24-25 -0.2154917619266925 2111 26-27 -0.11754738079717697 2111 28-29 -0.18205597035586152 2111 30-31 -0.2269245899256731 2111 32-33 -0.26822626180042874 2111 34-35 -0.1694403642707769 2111 36-37 -0.16101346167243946 2111 38-39 -0.17095859422914117 2111 40-41 0.9463639417412892 2111 42-43 -0.4686768185397199 2111 44-45 -0.4904183784109577 2111 46-47 0.10854495917901374 2111 48-49 -0.34584721368728566 2111 50-51 -0.6688161657944889 2111 52-53 -0.5143886052404909 2111 54-55 -0.47343917048664963 2111 56-57 -0.6430045713329307 2111 58-59 -0.454438156746086 2111 60-61 -0.5674452899136639 2111 62-63 -0.7256346623701404 2111 64-65 -0.5929048622265967 2111 66-67 -0.4169826750353778 2111 68-69 -0.39142359222884693 2111 70-71 -0.44239732860737035 2111 72-73 -0.7456551337690343 2111 74-75 -1.119846810367008 2111 76 -0.4989145563881223 2110 1 -0.03855187788091996 2110 2 -0.05300656200606113 2110 3 -0.025244102461833506 2110 4 -0.09647116693674462 2110 5 -0.09389755140859535 2110 6 0.02695894444301672 2110 7 6.519110868481448E-4 2110 8 -0.10417738573003277 2110 9 -0.19051099164708063 2110 10-11 -0.1655782935468011 2110 12-13 -0.23665048304533798 2110 14-15 -0.13846897606079978 2110 16-17 -0.2640871669207101 2110 18-19 -0.20440209933410358 2110 20-21 -0.3323154750881443 2110 22-23 -0.2161195808187415 2110 24-25 -0.2262616337615313 2110 26-27 -0.12548380395446657 2110 28-29 -0.3429508587393144 2110 30-31 -0.24491224552653534 2110 32-33 -0.19987820515193988 2110 34-35 -0.10434025997066243 2110 36-37 -0.22855536384327024 2110 38-39 -0.1576607228201965 2110 40-41 0.8220380574492836 2110 42-43 -0.19463061477693344 2110 44-45 -0.5917042416468163 2110 46-47 -0.23715745030087731 2110 48-49 -0.5514673458521386 2110 50-51 -0.7527031192433 2110 52-53 -0.7047653517771906 2110 54-55 -0.6133211568961201 2110 56-57 -0.7855300912399699 2110 58-59 -0.8478333113084986 2110 60-61 -0.5184754549936414 2110 62-63 -0.7918993433651735 2110 64-65 -0.623190685885838 2110 66-67 -1.107232242319462 2110 68-69 -1.17911212397874 2110 70-71 -0.7843557209845642 2110 72-73 -1.199450057596323 2110 74-75 -0.6779837958623531 2110 76 -0.7978591743547838 2109 1 -0.014047528072488547 2109 2 -0.039860984985409686 2109 3 -0.10850303924069493 2109 4 -0.06788376998271417 2109 5 -0.03958579920427496 2109 6 0.022917630455239646 2109 7 -0.20824901770571813 2109 8 -0.1133504073577356 2109 9 -0.22397859421504762 2109 10-11 -0.24789409772820648 2109 12-13 -0.2657076997562484 2109 14-15 -0.18218795196219872 2109 16-17 -0.3637512793465376 2109 18-19 -0.3196700923920659 2109 20-21 -0.41605461895171203 2109 22-23 -0.349294683588937 2109 24-25 -0.3916250431582924 2109 26-27 -0.4421941648083987 2109 28-29 -0.5711450999312291 2109 30-31 -0.4049669950172827 2109 32-33 -0.6952003226225045 2109 34-35 -0.29225768347212266 2109 36-37 -0.6581861816660961 2109 38-39 -0.3810172269420278 2109 40-41 -10.391749695431589 2109 42-43 -1.1840287380717527 2109 44-45 -0.6120640476519768 2109 46-47 -0.026990646362150983 2109 48-49 -0.29885038270233366 2109 50-51 -0.389626944280252 2109 52-53 -0.3969960901783125 2109 54-55 -0.703735675622724 2109 56-57 -0.4822248986791422 2109 58-59 -0.4572815511620121 2109 60-61 -0.6166673245655758 2109 62-63 -0.3702959995648385 2109 64-65 -0.5090096853195902 2109 66-67 -0.5033058324005779 2109 68-69 -0.8443786462646159 2109 70-71 -0.6753728885716939 2109 72-73 -0.4405991350545335 2109 74-75 -0.7189606091404812 2109 76 -0.6385177493237109 2108 1 -9.431986214067933E-4 2108 2 -0.08077208626802701 2108 3 -0.07686118951835397 2108 4 -0.013187224697936983 2108 5 -0.09813707936545768 2108 6 -0.10816792657459473 2108 7 -0.19291951748458303 2108 8 -0.2747204992541441 2108 9 -0.3397906734326952 2108 10-11 -0.3276621452981061 2108 12-13 -0.4104976157226403 2108 14-15 -0.34330053734722554 2108 16-17 -0.6076148598664659 2108 18-19 -0.6451032832122436 2108 20-21 -0.6621684328983406 2108 22-23 -0.34771819334372367 2108 24-25 -0.8427764510898825 2108 26-27 -0.6833499955617484 2108 28-29 -1.2536218801169952 2108 30-31 -0.7087170564453658 2108 32-33 -1.2851855798833114 2108 34-35 -0.7083641260491547 2108 36-37 -1.4073827023796355 2108 38-39 -0.6583468254481062 2108 40-41 -7.951135414631551 2108 42-43 -0.9097946233732443 2108 44-45 -0.48235791958671825 2108 46-47 0.23481828773780933 2108 48-49 -0.10983470625631497 2108 50-51 -0.23086631722241435 2108 52-53 -0.6408002083167972 2108 54-55 -0.6401890639956136 2108 56-57 -0.6598817859954664 2108 58-59 -0.5891189878910836 2108 60-61 -0.8381253814725511 2108 62-63 -0.6455483461174936 2108 64-65 -0.42271355283151024 2108 66-67 -0.5093400355555247 2108 68-69 -0.3526979739957028 2108 70-71 -0.38133485230455477 2108 72-73 -0.6160539485588856 2108 74-75 -0.5860454781758193 2108 76 -1.1746738257893838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 1.0 15 1.0 16 4.0 17 10.0 18 42.0 19 100.0 20 174.0 21 325.0 22 425.0 23 597.0 24 806.0 25 1227.0 26 1804.0 27 2850.0 28 4112.0 29 6081.0 30 9099.0 31 13602.0 32 19778.0 33 29639.0 34 44373.0 35 72100.0 36 155132.0 37 999838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.49349324069356 26.109775776325467 8.250876206389735 24.14585477659123 2 15.245697521954716 23.465739485890396 40.602824122949336 20.685738869205547 3 18.455395331695694 27.35268940667576 31.294186570659605 22.897728690968943 4 13.153300511995255 16.62442864882881 41.5446634001947 28.677607438981234 5 12.68645539826829 34.604536157554165 36.88627009915411 15.822738345023426 6 26.06279026401453 38.3454639158607 19.78457142605435 15.807174394070428 7 22.39703932540551 34.7899821014564 24.398034830947594 18.41494374219049 8 19.890509073342916 40.88414987791108 23.070547278437616 16.154793770308387 9 20.797182631218057 18.350632322214896 24.642726569279404 36.20945847728764 10-11 22.97976980035562 26.65847846228165 30.852082265758867 19.509669471603868 12-13 20.6367344481625 25.11625243553808 31.25505644868815 22.991956667611273 14-15 20.154802580091946 28.67393669583194 25.59260477402171 25.5786559500544 16-17 19.61670834183722 33.1468473455388 27.433335633665706 19.803108678958274 18-19 19.348450432486956 31.64977145953152 29.880253016983794 19.121525090997725 20-21 19.587232274348406 29.70049672496296 30.09150428522555 20.62076671546308 22-23 19.672393515411983 30.027302987544434 30.2131160057616 20.087187491281984 24-25 19.710642659027606 30.593772070343185 29.927385359020704 19.768199911608505 26-27 19.426894213587328 30.664837657713477 29.886199620885645 20.022068507813547 28-29 19.21865295472799 30.745043395525514 30.186025921319825 19.850277728426676 30-31 19.423810789341925 30.57666640726748 30.255402966841444 19.74411983654915 32-33 19.43078520132558 30.498993482228464 30.192082647516155 19.8781386689298 34-35 19.448955379914572 30.214988084767736 30.577730922780777 19.758325612536908 36-37 19.746836186479623 30.59270755482989 30.315860106510286 19.344596152180202 38-39 19.568107702540594 30.212565394289204 30.124357436411714 20.094969466758485 40-41 19.6380720669661 30.104865790288976 30.245235008317906 20.01182713442702 42-43 19.949571330615026 30.169030380538604 30.045252921544474 19.836145367301903 44-45 19.427041043313302 29.786281992361918 30.422715439586174 20.363961524738606 46-47 19.464923112613995 29.834148483028688 30.763580648429432 19.937347755927885 48-49 19.457508211452428 29.872581163801776 30.525863322081282 20.144047302664518 50-51 19.266372615668786 29.890824757253753 30.701655211500878 20.14114741557658 52-53 18.931674255316338 29.89262342139691 31.022074381002586 20.15362794228417 54-55 18.87767762359025 29.723512284509024 31.14287853804578 20.255931553854943 56-57 18.721267257998917 29.505323311715102 31.32443349420977 20.44897593607621 58-59 18.461048276145604 29.67307627363775 31.449826080189585 20.41604937002706 60-61 18.95457969256792 29.408892889183203 31.18395415388636 20.452573264362517 62-63 18.71139295892732 28.908093401325285 31.244631538144162 21.135882101603233 64-65 18.580420843360578 28.72363855807336 31.319991894999127 21.375948703566934 66-67 18.532664474988287 28.92501552724352 30.810749697897837 21.73157029987035 68-69 18.535601069507724 28.861144596445843 30.551044620085428 22.05220971396101 70-71 18.469858059703903 28.880672950000076 30.510079126539324 22.139389863756698 72-73 18.242308691879288 28.92490540494904 30.456045787381747 22.37674011578992 74-75 18.279603442276095 28.66409910419184 30.07263666543819 22.98366078809387 76 18.21055676363791 28.327712201990717 30.290091489602254 23.171639544769118 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.0 3 2.0 4 3.0 5 2.5 6 1.5 7 2.5 8 4.0 9 9.0 10 22.5 11 73.0 12 115.0 13 183.0 14 364.0 15 686.5 16 1203.0 17 1510.0 18 2156.5 19 3691.5 20 5868.0 21 7156.0 22 8843.0 23 12690.5 24 17381.0 25 19911.0 26 22687.0 27 28437.5 28 34407.5 29 37403.0 30 40922.0 31 47425.5 32 53009.5 33 55609.0 34 58163.5 35 63469.0 36 68756.5 37 71293.0 38 72691.5 39 75621.0 40 77830.5 41 78499.5 42 78490.0 43 78351.0 44 77036.5 45 72553.0 46 69245.0 47 65429.5 48 57353.0 49 49448.0 50 45804.0 51 42175.5 52 34585.5 53 27261.0 54 23898.0 55 21196.5 56 16391.5 57 12415.0 58 10542.0 59 9456.0 60 7231.0 61 5142.0 62 4192.0 63 3616.5 64 2538.5 65 1709.5 66 1165.5 67 948.0 68 762.0 69 475.5 70 320.0 71 265.0 72 194.0 73 101.0 74 55.5 75 32.0 76 27.0 77 15.5 78 7.0 79 5.0 80 3.5 81 1.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1362122.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.57295178756492 #Duplication Level Percentage of deduplicated Percentage of total 1 94.97940022170168 85.07585236870318 2 3.5792324490006355 6.412048311816437 3 0.6239108189457145 1.676566011154939 4 0.25567176322860513 0.9160509808447024 5 0.10678571193368012 0.47825557133181634 6 0.07046086159947261 0.3786832415375906 7 0.07672565847575659 0.4810780595262682 8 0.05492911853521396 0.3936130629030527 9 0.03791178590679104 0.30562835140885297 >10 0.20799909010841383 3.205146301357343 >50 0.005450764720259008 0.3337194689789846 >100 0.0015217558438340145 0.3433582704368513 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.873189038867297E-4 0.0 0.0 2 5.873189038867297E-4 0.0 0.0 3 5.873189038867297E-4 0.0 0.0 4 5.873189038867297E-4 0.0 0.0 5 6.607337668725709E-4 0.0 0.0 6 6.607337668725709E-4 0.0 0.0 7 6.607337668725709E-4 0.0 0.0 8 6.607337668725709E-4 0.0 0.0 9 6.607337668725709E-4 0.0 7.341486298584121E-5 10 9.543932188159357E-4 0.0 7.341486298584121E-5 11 9.543932188159357E-4 0.0 7.341486298584121E-5 12 9.543932188159357E-4 0.0 7.341486298584121E-5 13 9.543932188159357E-4 0.0 1.4682972597168241E-4 14 9.543932188159357E-4 0.0 1.4682972597168241E-4 15 9.543932188159357E-4 0.0 1.4682972597168241E-4 16 9.543932188159357E-4 0.0 1.4682972597168241E-4 17 9.543932188159357E-4 0.0 2.2024458895752362E-4 18 9.543932188159357E-4 0.0 2.2024458895752362E-4 19 0.001027808081801777 0.0 2.2024458895752362E-4 20 0.001027808081801777 0.0 4.4048917791504724E-4 21 0.001027808081801777 0.0 5.139040409008885E-4 22 0.001027808081801777 0.0 8.075634928442533E-4 23 0.001027808081801777 7.341486298584121E-5 8.809783558300945E-4 24 0.001027808081801777 7.341486298584121E-5 0.0013214675337451418 25 0.0011012229447876182 7.341486298584121E-5 0.0014682972597168241 26 0.0011012229447876182 7.341486298584121E-5 0.0016885418486743478 27 0.0011012229447876182 7.341486298584121E-5 0.0033770836973486956 28 0.0011746378077734593 7.341486298584121E-5 0.008662953832329262 29 0.0011746378077734593 7.341486298584121E-5 0.013875409104323989 30 0.0011746378077734593 7.341486298584121E-5 0.020849821087978903 31 0.0011746378077734593 1.4682972597168241E-4 0.027163499304761248 32 0.0011746378077734593 1.4682972597168241E-4 0.03406449642543032 33 0.0012480526707593005 2.2024458895752362E-4 0.040818663820127714 34 0.0012480526707593005 2.2024458895752362E-4 0.05395992429459329 35 0.0013214675337451418 2.2024458895752362E-4 0.07385535216375626 36 0.0014682972597168241 2.2024458895752362E-4 0.10894765667098835 37 0.0014682972597168241 2.2024458895752362E-4 0.17076297130506665 38 0.0014682972597168241 2.9365945194336483E-4 0.25966837038092033 39 0.0015417121227026655 3.6707431492920603E-4 0.3716260364343282 40 0.0016151269856885067 3.6707431492920603E-4 0.5128028179561008 41 0.0016885418486743478 3.6707431492920603E-4 0.6635235316660328 42 0.0016885418486743478 3.6707431492920603E-4 0.8338460137931845 43 0.0016885418486743478 3.6707431492920603E-4 1.0275878372128193 44 0.0016885418486743478 3.6707431492920603E-4 1.2485665748002015 45 0.001761956711660189 3.6707431492920603E-4 1.500746629156566 46 0.001761956711660189 3.6707431492920603E-4 1.7853026380896866 47 0.0018353715746460303 5.139040409008885E-4 2.0946728707120212 48 0.0019087864376318715 5.139040409008885E-4 2.4328951444877918 49 0.001982201300617713 5.139040409008885E-4 2.7965189608566634 50 0.002129031026589395 5.139040409008885E-4 3.21035854350785 51 0.0022024458895752363 5.139040409008885E-4 3.610469546780685 52 0.0022758607525610775 5.139040409008885E-4 4.0221066835422965 53 0.0022758607525610775 5.873189038867297E-4 4.441672625506379 54 0.002496105341518601 5.873189038867297E-4 4.889723534308968 55 0.0026429350674902837 5.873189038867297E-4 5.358991338514465 56 0.0026429350674902837 5.873189038867297E-4 5.82899329134982 57 0.002863179656447807 5.873189038867297E-4 6.306189900757788 58 0.0030100093824194894 6.607337668725709E-4 6.833895935900014 59 0.003156839108391172 6.607337668725709E-4 7.373421763982962 60 0.0033770836973486956 6.607337668725709E-4 7.972780705399369 61 0.0034504985603345368 6.607337668725709E-4 8.5440217542922 62 0.0036707431492920607 6.607337668725709E-4 9.116804515307733 63 0.003890987738249584 6.607337668725709E-4 9.679602854957192 64 0.003964402601235426 6.607337668725709E-4 10.256570263162917 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 370 0.0 21.756758 13 ATGCCGT 410 0.0 20.487804 14 TCTTAGC 130 1.05781925E-4 18.846153 2 GTGCAGG 205 3.2428943E-8 18.780487 1 CGTATGC 475 0.0 18.421051 11 TGCCGTC 510 0.0 17.843138 15 GCCGTCT 465 0.0 17.311829 16 GTGTAGC 310 4.9112714E-11 16.935484 1 GTACCGG 125 0.00176282 16.8 6 CTCGTAT 505 0.0 16.633663 9 GTATGCC 485 0.0 16.597939 12 CCTCTAG 235 1.5434307E-7 16.382978 1 GTCCTAT 215 1.0977456E-6 16.27907 1 GTATTAG 345 1.4551915E-11 16.231884 1 GGCCCTA 130 0.0022910899 16.153847 38 GTCCTAC 180 7.152403E-5 15.555556 1 GTGCTAG 180 7.152403E-5 15.555556 1 CTCTTAG 135 0.00294698 15.555555 1 ACTACGC 135 0.00294698 15.555555 8 TAGGACA 250 3.1177842E-7 15.4 4 >>END_MODULE