FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve14.3410000000c82c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve14.3410000000c82c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362122
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3700.021.75675813
ATGCCGT4100.020.48780414
TCTTAGC1301.05781925E-418.8461532
GTGCAGG2053.2428943E-818.7804871
CGTATGC4750.018.42105111
TGCCGTC5100.017.84313815
GCCGTCT4650.017.31182916
GTGTAGC3104.9112714E-1116.9354841
GTACCGG1250.0017628216.86
CTCGTAT5050.016.6336639
GTATGCC4850.016.59793912
CCTCTAG2351.5434307E-716.3829781
GTCCTAT2151.0977456E-616.279071
GTATTAG3451.4551915E-1116.2318841
GGCCCTA1300.002291089916.15384738
GTCCTAC1807.152403E-515.5555561
GTGCTAG1807.152403E-515.5555561
CTCTTAG1350.0029469815.5555551
ACTACGC1350.0029469815.5555558
TAGGACA2503.1177842E-715.44