##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve13.3410000000c802.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454398 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7422383694147 34.0 34.0 34.0 34.0 34.0 2 33.772736211133406 34.0 34.0 34.0 34.0 34.0 3 33.79539025768737 34.0 34.0 34.0 34.0 34.0 4 33.81559655610087 34.0 34.0 34.0 34.0 34.0 5 33.81724672338658 34.0 34.0 34.0 34.0 34.0 6 37.60209241211828 38.0 38.0 38.0 37.0 38.0 7 37.624393047845224 38.0 38.0 38.0 37.0 38.0 8 37.53650651334779 38.0 38.0 38.0 37.0 38.0 9 37.506699679179974 38.0 38.0 38.0 37.0 38.0 10-11 37.500117918203955 38.0 38.0 38.0 37.0 38.0 12-13 37.43830849602378 38.0 38.0 38.0 37.0 38.0 14-15 37.492678414024226 38.0 38.0 38.0 37.0 38.0 16-17 37.4391232661211 38.0 38.0 38.0 37.0 38.0 18-19 37.46020449698088 38.0 38.0 38.0 37.0 38.0 20-21 37.410050756395435 38.0 38.0 38.0 37.0 38.0 22-23 37.462080187128976 38.0 38.0 38.0 37.0 38.0 24-25 37.39361818429343 38.0 38.0 38.0 37.0 38.0 26-27 37.35703535070868 38.0 38.0 38.0 37.0 38.0 28-29 37.28202940323075 38.0 38.0 38.0 37.0 38.0 30-31 37.31641442026185 38.0 38.0 38.0 37.0 38.0 32-33 37.26609016239021 38.0 38.0 38.0 37.0 38.0 34-35 37.31040953026613 38.0 38.0 38.0 37.0 38.0 36-37 37.20913326338457 38.0 38.0 38.0 37.0 38.0 38-39 37.27438603463426 38.0 38.0 38.0 37.0 38.0 40-41 36.09358717489985 38.0 37.5 38.0 33.0 38.0 42-43 36.78610187857794 38.0 37.0 38.0 35.0 38.0 44-45 36.9927292941822 38.0 38.0 38.0 36.0 38.0 46-47 36.556631678536405 38.0 37.5 38.0 35.0 38.0 48-49 36.90029861152175 38.0 37.5 38.0 35.0 38.0 50-51 36.968594566274156 38.0 38.0 38.0 36.0 38.0 52-53 36.983244613922736 38.0 38.0 38.0 36.0 38.0 54-55 36.96839207699681 38.0 38.0 38.0 36.0 38.0 56-57 36.96747348387443 38.0 38.0 38.0 36.0 38.0 58-59 36.93875610390003 38.0 38.0 38.0 36.0 38.0 60-61 36.94130389343219 38.0 38.0 38.0 36.0 38.0 62-63 36.94803726352759 38.0 38.0 38.0 36.0 38.0 64-65 36.87103770769762 38.0 38.0 38.0 35.5 38.0 66-67 36.848012717289215 38.0 38.0 38.0 36.0 38.0 68-69 36.83953016987097 38.0 38.0 38.0 35.0 38.0 70-71 36.75828487112881 38.0 38.0 38.0 35.0 38.0 72-73 36.80853899689081 38.0 38.0 38.0 35.0 38.0 74-75 36.80583650417561 38.0 38.0 38.0 35.0 38.0 76 36.10879002858915 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0889168707291148 1101 2 0.08357186324817434 1101 3 0.05199716388451492 1101 4 0.024216843994494752 1101 5 0.055902706954043424 1101 6 0.07317455892938796 1101 7 0.0013800522532037007 1101 8 0.0958099801320742 1101 9 0.12997522610579182 1101 10-11 0.0773041568488253 1101 12-13 0.15009890557973193 1101 14-15 0.10515306458585627 1101 16-17 0.14780475531586745 1101 18-19 0.1334889997258344 1101 20-21 0.15055451702852451 1101 22-23 0.10753838595064735 1101 24-25 0.08416800975748373 1101 26-27 0.05598491982648568 1101 28-29 0.11312530510991081 1101 30-31 0.19658170192099078 1101 32-33 0.2502219286110261 1101 34-35 0.11336455358264175 1101 36-37 0.12926278517093692 1101 38-39 0.16329388902331488 1101 40-41 1.4133935020629593 1101 42-43 0.6071737473719523 1101 44-45 0.3455812557270619 1101 46-47 0.8610683712348077 1101 48-49 0.4893855410090353 1101 50-51 0.3244644989432146 1101 52-53 0.35148296206629936 1101 54-55 0.2417831883332937 1101 56-57 0.3274772174427554 1101 58-59 0.2709250675129411 1101 60-61 0.3205584313353853 1101 62-63 0.26933495235573446 1101 64-65 0.2348299594954497 1101 66-67 0.3000095611796496 1101 68-69 0.3416359297086444 1101 70-71 0.38013001031645643 1101 72-73 0.3853429279850076 1101 74-75 0.3118486736471837 1101 76 0.3122183342885094 1102 1 -0.012439387558032422 1102 2 0.06694972462656779 1102 3 0.04127571453548029 1102 4 0.08185558276632321 1102 5 0.048429938011324225 1102 6 0.07986701354997905 1102 7 0.07164220704512303 1102 8 0.07690793007562036 1102 9 0.09371744085479605 1102 10-11 0.10759824173004517 1102 12-13 0.1434435449738345 1102 14-15 0.09840573031463862 1102 16-17 0.16026487239773246 1102 18-19 0.15354373562954748 1102 20-21 0.1069049118422356 1102 22-23 0.07292324427130836 1102 24-25 0.14128974689273122 1102 26-27 0.19102860396748156 1102 28-29 0.15744004435268977 1102 30-31 0.19768788320303798 1102 32-33 0.21691792939505206 1102 34-35 0.15570593997174598 1102 36-37 0.047562821905579256 1102 38-39 0.10626704921957497 1102 40-41 1.3396380534607957 1102 42-43 0.6815386890085691 1102 44-45 0.31992626770586696 1102 46-47 0.8298882023163472 1102 48-49 0.4743882907779948 1102 50-51 0.3177388485295154 1102 52-53 0.37370325142031646 1102 54-55 0.3157504504593973 1102 56-57 0.35095849011220537 1102 58-59 0.4367603470638528 1102 60-61 0.3858305884156863 1102 62-63 0.3450487395675452 1102 64-65 0.32794473781790145 1102 66-67 0.4362213108702093 1102 68-69 0.3261001767126288 1102 70-71 0.45123482052088804 1102 72-73 0.4661767855986838 1102 74-75 0.44815023470828663 1102 76 0.6170676671109305 1103 1 0.0014661360503822607 1103 2 -0.024192636871276818 1103 3 0.009009285069318196 1103 4 0.03444491820312834 1103 5 0.015893270851336183 1103 6 0.08456297325844275 1103 7 0.1297164583301722 1103 8 0.10219810146111996 1103 9 0.12115535499574293 1103 10-11 0.12210328656368574 1103 12-13 0.14143043283098677 1103 14-15 0.07221006736109103 1103 16-17 0.17078839690023528 1103 18-19 0.1508296715823576 1103 20-21 0.16158511073669501 1103 22-23 0.13074242364096733 1103 24-25 0.18007733902788203 1103 26-27 0.16145527498756707 1103 28-29 0.2162225405421765 1103 30-31 0.21401919900977617 1103 32-33 0.20717295553847492 1103 34-35 0.17812227263019054 1103 36-37 0.1526107985557772 1103 38-39 0.17088763775551996 1103 40-41 1.4451506155771696 1103 42-43 0.6544216058424368 1103 44-45 0.3509037585781769 1103 46-47 0.808382823347344 1103 48-49 0.38976776327132256 1103 50-51 0.27337662749057756 1103 52-53 0.2661149916247396 1103 54-55 0.25814328684139554 1103 56-57 0.20019182492041665 1103 58-59 0.23648449467539479 1103 60-61 0.29750549149354555 1103 62-63 0.2506037475547487 1103 64-65 0.2982074231371499 1103 66-67 0.3644412238119301 1103 68-69 0.36184667935290804 1103 70-71 0.34027368549815407 1103 72-73 0.3325792768142861 1103 74-75 0.31329948946437725 1103 76 0.49119475755481545 2116 1 0.04595076810483789 2116 2 0.03258177328652323 2116 3 0.07208226373428062 2116 4 0.009689553920246397 2116 5 0.03968932127406788 2116 6 0.0069025026055697936 2116 7 0.11310778391085563 2116 8 0.12158704630743955 2116 9 0.14660204791152864 2116 10-11 0.12466288122839586 2116 12-13 0.17828877913626684 2116 14-15 0.095566902815726 2116 16-17 0.18155709940000975 2116 18-19 0.20381810365512365 2116 20-21 0.2212115185747976 2116 22-23 0.17691203570336 2116 24-25 0.20796535294602592 2116 26-27 0.16877649099342307 2116 28-29 0.13254812122770687 2116 30-31 0.150675030282855 2116 32-33 0.2125587565945466 2116 34-35 0.12161783164494011 2116 36-37 0.18195304438186355 2116 38-39 0.13235499422471264 2116 40-41 1.4302730097413203 2116 42-43 0.5530445951901726 2116 44-45 0.43878459977098316 2116 46-47 0.8202817642257685 2116 48-49 0.4257234144632207 2116 50-51 0.2676848410241348 2116 52-53 0.3220491246406354 2116 54-55 0.3246515431060857 2116 56-57 0.30315065042479716 2116 58-59 0.3685694685788832 2116 60-61 0.3671297627751784 2116 62-63 0.2784528456226951 2116 64-65 0.4181657602857669 2116 66-67 0.44561648248787833 2116 68-69 0.33783010577906225 2116 70-71 0.37740566364423955 2116 72-73 0.34895460874358974 2116 74-75 0.28216772497394516 2116 76 0.6269485041592162 1117 1 0.06379523441976431 1117 2 0.028791355025816756 1117 3 0.029141291846073614 1117 4 0.05790830153073756 1117 5 0.04441026292180794 1117 6 0.024617965943320996 1117 7 0.07114864060902448 1117 8 0.0821308814204258 1117 9 0.1490596409577023 1117 10-11 0.12301766161501604 1117 12-13 0.11951891995424546 1117 14-15 0.06862316340666297 1117 16-17 0.1628102905587383 1117 18-19 0.19485794658228173 1117 20-21 0.17044897038367424 1117 22-23 0.15994499929392703 1117 24-25 0.13621607997522744 1117 26-27 0.20240575419650497 1117 28-29 0.2278049185499711 1117 30-31 0.21526143144511423 1117 32-33 0.21736670742048858 1117 34-35 0.16139372905426796 1117 36-37 0.23330079250293068 1117 38-39 0.20570780133443378 1117 40-41 1.414976968874754 1117 42-43 0.5977760075384495 1117 44-45 0.3049197149733587 1117 46-47 0.7689145916602484 1117 48-49 0.4772951911770775 1117 50-51 0.23465070580040504 1117 52-53 0.22953980707378463 1117 54-55 0.2902990122551401 1117 56-57 0.26517209994467805 1117 58-59 0.24183107329699283 1117 60-61 0.3573985477545918 1117 62-63 0.33012593777225163 1117 64-65 0.3704706093670822 1117 66-67 0.3216968515893228 1117 68-69 0.2980867674385337 1117 70-71 0.35240282705869674 1117 72-73 0.3181923935429509 1117 74-75 0.36789390320524973 1117 76 0.6412688212648092 2117 1 0.011921570520797786 2117 2 0.07278242965294623 2117 3 0.04611167082317991 2117 4 0.09937328783921373 2117 5 0.05615757450875947 2117 6 -0.01027979250790878 2117 7 0.09218570370007484 2117 8 0.04897611039990579 2117 9 0.1304927874267321 2117 10-11 -0.0016654720510302923 2117 12-13 -0.07051371569697551 2117 14-15 -0.021833708692007292 2117 16-17 0.0853290188466147 2117 18-19 0.15833755494956137 2117 20-21 0.08270353828797283 2117 22-23 0.1374367283165796 2117 24-25 0.09606433660094638 2117 26-27 0.10538478590726186 2117 28-29 0.17367697657938663 2117 30-31 0.21239353091814905 2117 32-33 0.1669753964569125 2117 34-35 0.13565320962252514 2117 36-37 0.3122447367744954 2117 38-39 0.13921809635093751 2117 40-41 1.3708145736581727 2117 42-43 0.6547217740715965 2117 44-45 0.3935036968789518 2117 46-47 0.7773457445471053 2117 48-49 0.4074140139798814 2117 50-51 0.3037368080899725 2117 52-53 0.25044177866060835 2117 54-55 0.29444297485001414 2117 56-57 0.2636412368782146 2117 58-59 0.3989940117042252 2117 60-61 0.3876480531661741 2117 62-63 0.31679704239952144 2117 64-65 -0.08263642768446289 2117 66-67 0.24665048143049262 2117 68-69 0.30108926822789783 2117 70-71 0.37176773365090554 2117 72-73 0.2757458753483988 2117 74-75 0.2850328272554492 2117 76 0.5919164560766177 1116 1 0.036173527184530485 1116 2 0.07903354988027189 1116 3 0.053099204161483726 1116 4 -0.0018885496491165554 1116 5 0.07238081576024769 1116 6 0.05352030053389001 1116 7 0.10360044256879064 1116 8 0.0386763963054193 1116 9 0.1243524312498252 1116 10-11 0.04790513264683938 1116 12-13 0.02273451056780118 1116 14-15 0.09979998720897498 1116 16-17 0.11170596703522051 1116 18-19 0.11611765541590557 1116 20-21 0.1428799686378639 1116 22-23 0.13652812745450404 1116 24-25 0.09044826359602354 1116 26-27 0.18283268820397325 1116 28-29 0.19291434259684337 1116 30-31 0.16767640552048846 1116 32-33 0.16306408288254204 1116 34-35 0.17598172908309806 1116 36-37 0.27226092846477457 1116 38-39 0.18875177969866996 1116 40-41 1.3353522881460478 1116 42-43 0.5959899754580888 1116 44-45 0.29558559111875127 1116 46-47 0.6755347858876064 1116 48-49 0.4002435521340715 1116 50-51 0.19855929359498958 1116 52-53 0.2520438296080485 1116 54-55 0.25853662619029905 1116 56-57 0.24345062872987455 1116 58-59 0.2172083878038933 1116 60-61 0.18448496753482146 1116 62-63 0.2204339967774871 1116 64-65 -0.13058282862481718 1116 66-67 0.12710418440695292 1116 68-69 0.24446332916691915 1116 70-71 0.3199566742968116 1116 72-73 0.36784215548880894 1116 74-75 0.32385562692758896 1116 76 0.47309213927263727 2118 1 0.09193227535956083 2118 2 0.027251880578411658 2118 3 0.05227467294717769 2118 4 0.050038514041730764 2118 5 0.05981261034442298 2118 6 0.1206736788785463 2118 7 0.11338951894087757 2118 8 0.0852133865330984 2118 9 0.1298008818398344 2118 10-11 0.12226637993570222 2118 12-13 0.07017802201414014 2118 14-15 0.16396741817117544 2118 16-17 0.17829985728999986 2118 18-19 0.21036661265600287 2118 20-21 0.166074761105655 2118 22-23 0.1382784967446753 2118 24-25 0.11462870327329 2118 26-27 0.2467982976170049 2118 28-29 0.276891362971007 2118 30-31 0.2379591452017067 2118 32-33 0.22112897131368214 2118 34-35 0.20783364227764878 2118 36-37 0.24927658005496767 2118 38-39 0.20181049313382715 2118 40-41 1.3639379462509496 2118 42-43 0.6393714016722285 2118 44-45 0.3340665378421903 2118 46-47 0.7996120174858135 2118 48-49 0.37036198884335647 2118 50-51 0.2379160495622088 2118 52-53 0.3037776942585424 2118 54-55 0.2696924159124663 2118 56-57 0.2599679736528344 2118 58-59 0.17551901468953446 2118 60-61 0.3005971032924748 2118 62-63 0.2548288188360175 2118 64-65 0.3345865317684442 2118 66-67 0.2511090234929654 2118 68-69 0.22174822849714815 2118 70-71 0.2521130382232357 2118 72-73 0.28676281603757303 2118 74-75 0.28873721489755866 2118 76 0.5666444099874823 1119 1 0.01173772326458078 1119 2 0.04292125091079413 1119 3 0.0016251788069041595 1119 4 -0.024887930497030197 1119 5 0.01887729316474207 1119 6 0.051638293161964555 1119 7 -0.00545026866217313 1119 8 0.09997917095934383 1119 9 0.11113202568749614 1119 10-11 0.051376610549077384 1119 12-13 0.10985109645399405 1119 14-15 0.09998161655490634 1119 16-17 0.1237671571923471 1119 18-19 0.14453568579894238 1119 20-21 0.09335427529195783 1119 22-23 0.09536889617047706 1119 24-25 0.055526937684703626 1119 26-27 0.16249499564419523 1119 28-29 0.18529128443990572 1119 30-31 0.21910377155418814 1119 32-33 0.18427300390040813 1119 34-35 0.17044182027886023 1119 36-37 0.1857053537324802 1119 38-39 0.23024919945044076 1119 40-41 1.3567221349630003 1119 42-43 0.5496847829474731 1119 44-45 0.2679836713442043 1119 46-47 0.6926875217544435 1119 48-49 0.3221142293525787 1119 50-51 0.216802416261487 1119 52-53 0.23772027311246546 1119 54-55 0.22646658786246832 1119 56-57 0.16043734535865894 1119 58-59 0.2636456494001109 1119 60-61 0.2288660737833439 1119 62-63 0.22340803990413605 1119 64-65 0.19074824942658353 1119 66-67 0.12957984613566964 1119 68-69 0.15728359440928585 1119 70-71 0.24184258908150724 1119 72-73 0.17416661268028832 1119 74-75 0.21965394286811346 1119 76 0.5592162679678339 2119 1 -0.018822811072752188 2119 2 0.037322081707060306 2119 3 0.045025145836838476 2119 4 0.02241732230125848 2119 5 0.016344340077935726 2119 6 0.06345256673077415 2119 7 0.10057115200191191 2119 8 0.09052194838484695 2119 9 0.15388598935003728 2119 10-11 0.10612852932663941 2119 12-13 0.08951254821118937 2119 14-15 0.11310746897620305 2119 16-17 0.02117857483726482 2119 18-19 0.10251453432865532 2119 20-21 0.10206689301021754 2119 22-23 0.13835528644838035 2119 24-25 0.1351167999451519 2119 26-27 0.09371329514205229 2119 28-29 0.17863725835650968 2119 30-31 0.16880867711717684 2119 32-33 0.17391836577157704 2119 34-35 0.17769717961245135 2119 36-37 0.20609138133966098 2119 38-39 0.15651393179093276 2119 40-41 1.3761549016516241 2119 42-43 0.5404982050982952 2119 44-45 0.35451231239094483 2119 46-47 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66-67 -0.3582840850967415 2104 68-69 -0.5569470647065344 2104 70-71 -0.40034326775510465 2104 72-73 -0.2763060597449467 2104 74-75 -0.27117471774204915 2104 76 -0.6179817740273208 2111 1 -0.18611251193742362 2111 2 -0.07488644426938862 2111 3 -0.08343116412365248 2111 4 -0.05417704969314485 2111 5 -0.14058316288656414 2111 6 -0.028313164473154018 2111 7 -0.05216134017410923 2111 8 -0.09956511395849077 2111 9 -0.2555991473351398 2111 10-11 -0.13233036266884568 2111 12-13 -0.08045115100733824 2111 14-15 -0.07775119264361052 2111 16-17 -0.08540485292840572 2111 18-19 -0.15022173052583554 2111 20-21 -0.06532619308921994 2111 22-23 -0.1847809584697515 2111 24-25 -0.19606404149367052 2111 26-27 -0.15342054218420742 2111 28-29 -0.1597416113213299 2111 30-31 -0.16417311642108956 2111 32-33 -0.24600563379370755 2111 34-35 -0.18627979804983852 2111 36-37 -0.15568962055913715 2111 38-39 -0.22391445065760252 2111 40-41 0.9679364850274723 2111 42-43 -0.3440496307341263 2111 44-45 -0.3566053211082263 2111 46-47 0.16983714186785903 2111 48-49 -0.2627366614772555 2111 50-51 -0.5253232978257998 2111 52-53 -0.5332662938201409 2111 54-55 -0.4121672810610164 2111 56-57 -0.6135548714378132 2111 58-59 -0.4639792940006586 2111 60-61 -0.5478842444538259 2111 62-63 -0.6442936221496183 2111 64-65 -0.6107045490900873 2111 66-67 -0.3101340173752334 2111 68-69 -0.35364546522639273 2111 70-71 -0.4053599828315484 2111 72-73 -0.7024232296906874 2111 74-75 -1.067724835569642 2111 76 -0.4082330090639985 2110 1 -0.05150940999625675 2110 2 -0.09825563630418088 2110 3 -0.08648623829339641 2110 4 -0.0658905288591285 2110 5 -0.06415072220600848 2110 6 -0.06022195911941708 2110 7 -0.04686754950608929 2110 8 -0.10792923781814778 2110 9 -0.19119844542013453 2110 10-11 -0.18945142120899305 2110 12-13 -0.2685714574956961 2110 14-15 -0.14749091732726072 2110 16-17 -0.2800058840023141 2110 18-19 -0.21468183363322169 2110 20-21 -0.4262423883611035 2110 22-23 -0.2419903013508673 2110 24-25 -0.2436661951956154 2110 26-27 -0.16556127396329856 2110 28-29 -0.3170850156091163 2110 30-31 -0.20994309756530072 2110 32-33 -0.2818964881899362 2110 34-35 -0.15911819410865036 2110 36-37 -0.2507113646653423 2110 38-39 -0.19696374819762497 2110 40-41 0.7499989598228396 2110 42-43 -0.19531340472621395 2110 44-45 -0.4544341802783478 2110 46-47 -0.29704860075604245 2110 48-49 -0.45429351768277115 2110 50-51 -0.7004450975184398 2110 52-53 -0.6690040130323425 2110 54-55 -0.5984347063190611 2110 56-57 -0.73421504212088 2110 58-59 -0.8520145859384485 2110 60-61 -0.43376910423430814 2110 62-63 -0.6858161455558758 2110 64-65 -0.5157476558269494 2110 66-67 -1.0151417013826887 2110 68-69 -1.0777945022731217 2110 70-71 -0.7708206418033186 2110 72-73 -1.1628571308776259 2110 74-75 -0.6368945267924389 2110 76 -0.9100597319710602 2109 1 -0.10042226185028369 2109 2 -0.10984159936072757 2109 3 -0.19732246089432692 2109 4 -0.1688086891302305 2109 5 -0.05117889011653176 2109 6 -0.02383628596903975 2109 7 -0.1936947419215187 2109 8 -0.1931043568167965 2109 9 -0.26201831182823554 2109 10-11 -0.367252541657173 2109 12-13 -0.35397820760832843 2109 14-15 -0.28831308535531264 2109 16-17 -0.5006298678615906 2109 18-19 -0.4795730698479943 2109 20-21 -0.49889424630708135 2109 22-23 -0.4577933708304087 2109 24-25 -0.46647059667649415 2109 26-27 -0.4890728114454248 2109 28-29 -0.6049718836430458 2109 30-31 -0.4369545664885308 2109 32-33 -0.6837412120934871 2109 34-35 -0.3268526655195956 2109 36-37 -0.7830079896090254 2109 38-39 -0.4712345754096319 2109 40-41 -10.787070343422933 2109 42-43 -1.3428626691872338 2109 44-45 -0.6919089604001911 2109 46-47 -0.1422656386201382 2109 48-49 -0.4602623037986149 2109 50-51 -0.5969982992186971 2109 52-53 -0.4442187492764873 2109 54-55 -0.6200510801413159 2109 56-57 -0.42466699213164816 2109 58-59 -0.5240491690024385 2109 60-61 -0.7308480057249795 2109 62-63 -0.5147781149590145 2109 64-65 -0.743869778802015 2109 66-67 -0.7717625315329286 2109 68-69 -1.038150567776242 2109 70-71 -0.832331924638126 2109 72-73 -0.6015172452730368 2109 74-75 -0.873984642559023 2109 76 -0.5872888753766716 2108 1 0.024397362969338587 2108 2 -0.11393472845385588 2108 3 -0.027547791119658882 2108 4 -0.019499664821211127 2108 5 -0.08810030203794383 2108 6 -0.07624221337496806 2108 7 -0.16454333777011243 2108 8 -0.24193053064297487 2108 9 -0.1840268588367806 2108 10-11 -0.3011658505704844 2108 12-13 -0.3893733680034899 2108 14-15 -0.38099124053346856 2108 16-17 -0.6483155155472389 2108 18-19 -0.6754535782284989 2108 20-21 -0.7486028710157058 2108 22-23 -0.38494261047964784 2108 24-25 -0.8931203358262252 2108 26-27 -0.7842012940739096 2108 28-29 -1.374403840574999 2108 30-31 -0.7324992995332664 2108 32-33 -1.217998621350894 2108 34-35 -0.7485906497575741 2108 36-37 -1.4914245730256042 2108 38-39 -0.692943422118482 2108 40-41 -7.89078607213866 2108 42-43 -0.9443653331899071 2108 44-45 -0.43928658277781096 2108 46-47 0.15625528490078722 2108 48-49 -0.17363920467551708 2108 50-51 -0.31344157816198503 2108 52-53 -0.5884287059864377 2108 54-55 -0.6377347853250157 2108 56-57 -0.5479118028764631 2108 58-59 -0.5195467270000051 2108 60-61 -0.7468320217089968 2108 62-63 -0.5766676143485157 2108 64-65 -0.46923469166691945 2108 66-67 -0.5394809242513219 2108 68-69 -0.3978117399374099 2108 70-71 -0.45106735587355473 2108 72-73 -0.6111118173676076 2108 74-75 -0.5668126478870263 2108 76 -1.1655578286456247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 0.0 15 2.0 16 3.0 17 8.0 18 28.0 19 67.0 20 136.0 21 215.0 22 356.0 23 511.0 24 733.0 25 1070.0 26 1767.0 27 2684.0 28 4166.0 29 6268.0 30 9385.0 31 13834.0 32 20839.0 33 31195.0 34 47359.0 35 76642.0 36 165616.0 37 1071511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.52116408300891 27.280084268542726 8.344414665036668 23.854336983411695 2 15.19556545044754 23.74370701829898 41.10188545363786 19.958842077615618 3 18.51308926442418 26.42681026789091 32.69957741966092 22.360523048023996 4 13.726297753434755 16.81740486441813 41.53532939401732 27.920967988129796 5 13.604529159143508 34.82348023030835 36.287522397583054 15.284468212965088 6 26.8385270056752 39.247578723293074 18.730980103107953 15.182914167923773 7 23.01667081500387 35.48155319245488 23.74432583103112 17.757450161510125 8 19.917244110621716 41.87113843665902 22.62833144710045 15.583286005618818 9 20.522442962655337 18.65658506131059 26.318861824617475 34.5021101514166 10-11 23.162676241303963 27.28053118884927 30.624973356674033 18.931819213172734 12-13 20.44577894084013 25.623522584602014 31.72955408354522 22.20114439101264 14-15 19.695227853723672 29.595234591906756 26.045896652773177 24.6636409015964 16-17 18.82280503686061 34.54198919415456 28.129129715524908 18.506076053459918 18-19 18.26790190855598 32.766374816246994 31.019982150690527 17.945741124506497 20-21 18.51982744750749 30.671281176129227 31.672313905822204 19.136577470541077 22-23 18.917586520333497 30.960988670226445 31.258740729841488 18.86268407959857 24-25 18.75191660054538 31.37442433226668 31.222815212892208 18.65084385429573 26-27 18.47520417382312 31.70566103638756 31.124836530303256 18.694298259486057 28-29 18.190034639761606 31.759635258024282 31.427401577834953 18.62292852437916 30-31 18.403284382954322 31.438127665192063 31.51156010940609 18.647027842447528 32-33 18.5207556666057 31.474671994873482 31.39285120029043 18.61172113823039 34-35 18.430787171049463 31.381575057171418 31.72089070529525 18.466747066483865 36-37 18.50734805740932 31.550992231837505 31.70789563792029 18.233764072832884 38-39 18.404487629933485 31.4272984423796 31.46982462847171 18.698389299215208 40-41 18.533303813674113 31.371020862239913 31.37494000954347 18.720735314542512 42-43 18.64888428064395 31.337261189853123 31.42265734688854 18.591197182614387 44-45 18.314553512862368 31.008465358175684 31.81821619666694 18.85876493229501 46-47 18.38788282162104 30.92606012934561 32.00241611993416 18.683640929099187 48-49 18.298533138796948 31.175476038883442 31.80412101776818 18.721869804551435 50-51 18.1081107097232 31.157805497532316 31.8728092310358 18.861274561708694 52-53 17.91930406945004 30.94548397343781 32.229795420510754 18.9054165366014 54-55 17.873168142420436 30.831553673753675 32.30673447020692 18.98854371361897 56-57 17.687421187322865 30.8058041883996 32.41602367439999 19.090750949877545 58-59 17.527767502430557 31.01276266881555 32.378998045926906 19.080471782826987 60-61 17.739504592278042 30.596886134331868 32.39780307728696 19.26580619610313 62-63 17.58514519409405 30.2369777736218 32.44483284492966 19.733044187354494 64-65 17.536327745225172 29.969031860604865 32.37827609773941 20.11636429643055 66-67 17.534574442484107 30.053259149146243 32.04415160086854 20.368014807501112 68-69 17.44388399874037 30.171108596133934 31.829217311904994 20.5557900932207 70-71 17.512812861403827 30.10004826739311 31.706898661851845 20.680240209351222 72-73 17.27164778829454 30.02228413405409 31.742480393949933 20.963587683701434 74-75 17.518313419022853 29.98154562918816 31.344136886876907 21.156004064912082 76 17.574006564915518 29.739314823040186 31.21923985043984 21.467438761604456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 6.0 2 5.0 3 8.5 4 9.0 5 10.0 6 13.5 7 17.5 8 19.0 9 47.5 10 134.5 11 314.0 12 435.0 13 664.0 14 1160.5 15 1930.5 16 3264.0 17 4095.0 18 5321.5 19 8187.0 20 11918.0 21 14010.0 22 16758.5 23 22684.0 24 29392.0 25 32923.0 26 36330.5 27 43583.0 28 50271.0 29 53114.0 30 56686.5 31 62532.5 32 66224.0 33 67642.0 34 69431.5 35 73391.0 36 76543.5 37 77526.0 38 77847.0 39 78405.0 40 78140.5 41 76266.5 42 74894.0 43 73614.5 44 70164.5 45 64604.0 46 61214.0 47 57509.5 48 49436.5 49 41865.0 50 38662.0 51 35138.0 52 28305.0 53 22204.5 54 19413.0 55 17243.5 56 13330.0 57 9766.5 58 7947.0 59 7187.0 60 5424.5 61 3712.5 62 3003.0 63 2583.0 64 1783.5 65 1139.0 66 720.5 67 567.0 68 472.5 69 291.5 70 167.0 71 129.0 72 100.0 73 56.0 74 33.0 75 25.0 76 22.0 77 12.5 78 6.5 79 7.0 80 5.0 81 2.5 82 1.5 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1454398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.66593084189356 #Duplication Level Percentage of deduplicated Percentage of total 1 95.6092149202682 87.64107682729043 2 3.2589700945945252 5.97473054613802 3 0.4785741442114272 1.3160683321800921 4 0.21595305416067154 0.7918215091115125 5 0.09074427305312437 0.41590791289928014 6 0.06893514369874419 0.3791402468919048 7 0.06714997280354063 0.4308755334131072 8 0.02945893717969467 0.21603047185516627 9 0.04633711105853483 0.3822780975934315 >10 0.13036709610761743 2.0398594647519803 >50 0.0029395973642800245 0.19770810057532098 >100 0.0013556554995347203 0.21450295729981222 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.188127321407207E-4 6.875697023785786E-5 0.0 2 6.188127321407207E-4 6.875697023785786E-5 0.0 3 6.188127321407207E-4 6.875697023785786E-5 0.0 4 6.188127321407207E-4 6.875697023785786E-5 0.0 5 6.875697023785787E-4 6.875697023785786E-5 0.0 6 6.875697023785787E-4 6.875697023785786E-5 0.0 7 6.875697023785787E-4 6.875697023785786E-5 0.0 8 6.875697023785787E-4 6.875697023785786E-5 6.875697023785786E-5 9 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 10 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 11 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 12 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 13 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 14 7.563266726164365E-4 6.875697023785786E-5 6.875697023785786E-5 15 8.938406130921523E-4 6.875697023785786E-5 6.875697023785786E-5 16 8.938406130921523E-4 6.875697023785786E-5 1.3751394047571572E-4 17 8.938406130921523E-4 6.875697023785786E-5 1.3751394047571572E-4 18 8.938406130921523E-4 1.3751394047571572E-4 1.3751394047571572E-4 19 8.938406130921523E-4 1.3751394047571572E-4 1.3751394047571572E-4 20 9.625975833300101E-4 1.3751394047571572E-4 2.7502788095143144E-4 21 9.625975833300101E-4 2.7502788095143144E-4 2.7502788095143144E-4 22 9.625975833300101E-4 2.7502788095143144E-4 3.4378485118928933E-4 23 0.001031354553567868 2.7502788095143144E-4 4.1254182142714716E-4 24 0.001031354553567868 2.7502788095143144E-4 6.188127321407207E-4 25 0.001031354553567868 2.7502788095143144E-4 7.563266726164365E-4 26 0.001031354553567868 2.7502788095143144E-4 9.625975833300101E-4 27 0.0011001115238057258 2.7502788095143144E-4 0.004262932154747188 28 0.0011001115238057258 2.7502788095143144E-4 0.012101226761862984 29 0.0011688684940435836 2.7502788095143144E-4 0.020145792279692355 30 0.0011688684940435836 3.4378485118928933E-4 0.025921377779672416 31 0.0012376254642814414 4.1254182142714716E-4 0.03265956086298249 32 0.0012376254642814414 4.1254182142714716E-4 0.03987904273795756 33 0.0013063824345192995 4.1254182142714716E-4 0.04888620583911694 34 0.0013063824345192995 4.1254182142714716E-4 0.06243132897597494 35 0.0013063824345192995 4.8129879166500505E-4 0.08470858733304089 36 0.0013063824345192995 4.8129879166500505E-4 0.12355627551743058 37 0.0013063824345192995 4.8129879166500505E-4 0.18612511843388124 38 0.0013751394047571573 4.8129879166500505E-4 0.2726901439633443 39 0.0013751394047571573 4.8129879166500505E-4 0.383113838165344 40 0.0013751394047571573 4.8129879166500505E-4 0.5011007990935081 41 0.0013751394047571573 4.8129879166500505E-4 0.625963457045458 42 0.0013751394047571573 6.188127321407207E-4 0.7660901623902123 43 0.0014438963749950151 6.188127321407207E-4 0.932619544306304 44 0.0015814103154707308 6.188127321407207E-4 1.1211511566985104 45 0.0015814103154707308 6.188127321407207E-4 1.3411734614596555 46 0.0015814103154707308 6.188127321407207E-4 1.6017623786611368 47 0.0017189242559464465 6.188127321407207E-4 1.8874475899994363 48 0.0017189242559464465 6.188127321407207E-4 2.195203788784088 49 0.0017876812261843045 6.188127321407207E-4 2.516573867675836 50 0.0017876812261843045 6.188127321407207E-4 2.869365881966284 51 0.0017876812261843045 6.188127321407207E-4 3.212531920423433 52 0.0018564381964221624 6.188127321407207E-4 3.5846446433507198 53 0.0018564381964221624 6.875697023785787E-4 3.9582012626530014 54 0.0018564381964221624 6.875697023785787E-4 4.349909722098078 55 0.0018564381964221624 6.875697023785787E-4 4.759770021685949 56 0.0018564381964221624 6.875697023785787E-4 5.189569842642798 57 0.0018564381964221624 7.563266726164365E-4 5.654229447510241 58 0.0019251951666600202 7.563266726164365E-4 6.146460597443066 59 0.0019251951666600202 7.563266726164365E-4 6.63999812981041 60 0.0019251951666600202 7.563266726164365E-4 7.17472108735023 61 0.0019251951666600202 7.563266726164365E-4 7.698511686622231 62 0.001993952136897878 7.563266726164365E-4 8.244029488489396 63 0.001993952136897878 7.563266726164365E-4 8.774076972053042 64 0.002131466077373594 7.563266726164365E-4 9.326401713973754 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTGCG 75 5.589074E-5 28.0 9 ATAGGGC 175 2.0190782E-10 24.0 3 CCTACAC 310 0.0 22.580645 3 CGTATGC 280 0.0 20.0 11 AAGGCCG 195 1.8271749E-8 19.74359 5 GTGTAGC 325 0.0 19.384615 1 GTGTAGA 530 0.0 19.150942 1 CATAGGG 165 1.5937476E-6 19.090908 2 GTGTATG 480 0.0 18.958334 1 CGTACAC 260 7.2759576E-11 18.846153 3 GAGTTAG 245 6.6211214E-10 18.571428 1 CTCTTAG 190 3.0083902E-7 18.421051 1 GACTAGG 820 0.0 17.92683 70 GTCCAGG 235 7.93807E-9 17.872341 1 GTATAGA 395 0.0 17.72152 1 CAGCGCT 120 0.0013413 17.500002 9 GTGCTAC 320 3.6379788E-12 17.5 1 GCCCTAT 140 1.866107E-4 17.5 1 CTACACT 425 0.0 17.294117 4 GTCTTAG 205 6.673381E-7 17.073172 1 >>END_MODULE