FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve13.3410000000c802.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve13.3410000000c802.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454398
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCG755.589074E-528.09
ATAGGGC1752.0190782E-1024.03
CCTACAC3100.022.5806453
CGTATGC2800.020.011
AAGGCCG1951.8271749E-819.743595
GTGTAGC3250.019.3846151
GTGTAGA5300.019.1509421
CATAGGG1651.5937476E-619.0909082
GTGTATG4800.018.9583341
CGTACAC2607.2759576E-1118.8461533
GAGTTAG2456.6211214E-1018.5714281
CTCTTAG1903.0083902E-718.4210511
GACTAGG8200.017.9268370
GTCCAGG2357.93807E-917.8723411
GTATAGA3950.017.721521
CAGCGCT1200.001341317.5000029
GTGCTAC3203.6379788E-1217.51
GCCCTAT1401.866107E-417.51
CTACACT4250.017.2941174
GTCTTAG2056.673381E-717.0731721
Produced by FastQC (version 0.11.2)