FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve12.3410000000c7e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve12.3410000000c7e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558060
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTC5350.018.97196270
GCTACAC3001.8189894E-1218.6666663
TAGGGCG1000.00969187517.55
GACGGAC9750.017.2307770
ACAGCGC1854.7519497E-617.0270278
ACAGACC2351.543849E-716.3829788
GCCCTAT1300.002291288716.1538471
ACGGACT7200.016.04166670
TAGGGCC1755.6229175E-516.05
GGTATAT4300.015.46511651
CGCGTAA1605.163585E-415.312510
TATACAG5300.015.188685
CTAGGGC3009.746145E-915.1666674
GTATAGG2806.8765075E-815.0000011
TGGACAG4450.014.943825
TACACAG4450.014.943825
CATACCG2352.7725546E-614.8936185
TTAGTAC4009.094947E-1214.8749993
ACTGCTC6600.014.8484858
ACACCAC7100.014.7887336