##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve12.3410000000c7e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558060 Sequences flagged as poor quality 0 Sequence length 76 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.79765862675379 34.0 34.0 34.0 34.0 34.0 2 33.820175731358226 34.0 34.0 34.0 34.0 34.0 3 33.84044131804937 34.0 34.0 34.0 34.0 34.0 4 33.852529427621526 34.0 34.0 34.0 34.0 34.0 5 33.85542276934136 34.0 34.0 34.0 34.0 34.0 6 37.685446003363154 38.0 38.0 38.0 38.0 38.0 7 37.704222558823155 38.0 38.0 38.0 38.0 38.0 8 37.63948307510622 38.0 38.0 38.0 37.0 38.0 9 37.61865589258437 38.0 38.0 38.0 37.0 38.0 10-11 37.61480494974519 38.0 38.0 38.0 37.0 38.0 12-13 37.56386852881147 38.0 38.0 38.0 37.0 38.0 14-15 37.60527129892302 38.0 38.0 38.0 37.0 38.0 16-17 37.56484731011643 38.0 38.0 38.0 37.0 38.0 18-19 37.58133383823473 38.0 38.0 38.0 37.0 38.0 20-21 37.54164858862945 38.0 38.0 38.0 37.0 38.0 22-23 37.58254913161239 38.0 38.0 38.0 37.0 38.0 24-25 37.526792292979735 38.0 38.0 38.0 37.0 38.0 26-27 37.502588154499826 38.0 38.0 38.0 37.0 38.0 28-29 37.44632555870763 38.0 38.0 38.0 37.0 38.0 30-31 37.4695047045685 38.0 38.0 38.0 37.0 38.0 32-33 37.42930054041564 38.0 38.0 38.0 37.0 38.0 34-35 37.464487246960964 38.0 38.0 38.0 37.0 38.0 36-37 37.382698997471216 38.0 38.0 38.0 37.0 38.0 38-39 37.43603872764848 38.0 38.0 38.0 37.0 38.0 40-41 36.23253340692913 38.0 37.5 38.0 34.5 38.0 42-43 36.998646393592026 38.0 37.5 38.0 35.0 38.0 44-45 37.20832156656355 38.0 38.0 38.0 37.0 38.0 46-47 36.79948461548336 38.0 38.0 38.0 35.5 38.0 48-49 37.125220145565635 38.0 38.0 38.0 36.5 38.0 50-51 37.18790643492548 38.0 38.0 38.0 37.0 38.0 52-53 37.203367649512856 38.0 38.0 38.0 37.0 38.0 54-55 37.1957928449482 38.0 38.0 38.0 37.0 38.0 56-57 37.19697572622364 38.0 38.0 38.0 37.0 38.0 58-59 37.18038811085581 38.0 38.0 38.0 37.0 38.0 60-61 37.17901974250029 38.0 38.0 38.0 37.0 38.0 62-63 37.186720344531025 38.0 38.0 38.0 37.0 38.0 64-65 37.12322760355827 38.0 38.0 38.0 36.5 38.0 66-67 37.10496867899825 38.0 38.0 38.0 37.0 38.0 68-69 37.09564297908938 38.0 38.0 38.0 36.0 38.0 70-71 37.03017630899966 38.0 38.0 38.0 36.0 38.0 72-73 37.06952203381128 38.0 38.0 38.0 36.0 38.0 74-75 37.06645957151842 38.0 38.0 38.0 36.0 38.0 76 36.46341604302787 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0418469571038429 1101 2 0.05348779449731467 1101 3 0.029076780210765207 1101 4 0.02821299160437718 1101 5 0.022393131132943722 1101 6 0.03971548836027239 1101 7 0.024906507401723843 1101 8 0.024747385707222236 1101 9 0.04828495978340186 1101 10-11 0.037136466258026246 1101 12-13 0.0876063607460793 1101 14-15 0.07502757114945524 1101 16-17 0.06350948319117578 1101 18-19 0.03720802340908591 1101 20-21 0.120920817911923 1101 22-23 0.04993351224098319 1101 24-25 0.030953284011616233 1101 26-27 0.09197881405051334 1101 28-29 0.08748910307840418 1101 30-31 0.09481317640268827 1101 32-33 0.12262097658283722 1101 34-35 0.013641803762830307 1101 36-37 0.0032121417965029764 1101 38-39 0.06511709300220048 1101 40-41 1.2936905086912418 1101 42-43 0.43027245609537346 1101 44-45 0.17940306003132633 1101 46-47 0.635905324930647 1101 48-49 0.3093997935237667 1101 50-51 0.10816476287988053 1101 52-53 0.17027091948687456 1101 54-55 0.15453579436835696 1101 56-57 0.13349839282285814 1101 58-59 0.10607950018285095 1101 60-61 0.17792350305182936 1101 62-63 0.13927298291250878 1101 64-65 0.1013209516827942 1101 66-67 0.13435045403559087 1101 68-69 0.16482349327083767 1101 70-71 0.21558999936124223 1101 72-73 0.19164007018525808 1101 74-75 0.16467820231819985 1101 76 0.07925030359864138 1102 1 -2.641260339260043E-4 1102 2 0.04548640104010815 1102 3 0.021820304241842337 1102 4 0.0364512524012639 1102 5 0.039988756057837804 1102 6 -0.013174106317407563 1102 7 0.02757326104621427 1102 8 -0.026137811833798708 1102 9 0.04168670661066187 1102 10-11 0.05273791507298853 1102 12-13 0.05183545693437708 1102 14-15 0.03532026964434465 1102 16-17 0.03233501190705823 1102 18-19 0.07662786207654904 1102 20-21 0.06241005491595786 1102 22-23 0.025508910744939328 1102 24-25 0.09065750052207022 1102 26-27 0.08242504199334633 1102 28-29 0.09012895928413656 1102 30-31 0.09961060358055818 1102 32-33 0.09726079722732095 1102 34-35 0.04617569991516035 1102 36-37 -0.013737236770730021 1102 38-39 0.06428109229233314 1102 40-41 1.2821440044919044 1102 42-43 0.5281974234532001 1102 44-45 0.24985467199200428 1102 46-47 0.6394352598547002 1102 48-49 0.3014055349661078 1102 50-51 0.22126237725260722 1102 52-53 0.20365947467765722 1102 54-55 0.17485109010937094 1102 56-57 0.15578195819331597 1102 58-59 0.19028348945241902 1102 60-61 0.21085913368691678 1102 62-63 0.1417369864949123 1102 64-65 0.1808208054173548 1102 66-67 0.18995173523704523 1102 68-69 0.07822432954901615 1102 70-71 0.21371640871625175 1102 72-73 0.2285963299667202 1102 74-75 0.2634846129008608 1102 76 0.3248437386756464 1103 1 -0.051991712131311374 1103 2 -0.019275063382885094 1103 3 0.0030768258989226638 1103 4 0.05123168054877425 1103 5 0.010892211828405607 1103 6 0.05633427293314952 1103 7 0.05097182744387396 1103 8 0.05664241286793015 1103 9 0.08316617170475382 1103 10-11 0.07634832744654574 1103 12-13 0.12921136536352407 1103 14-15 0.012229000736432738 1103 16-17 0.0632403019116552 1103 18-19 0.11523946658715545 1103 20-21 0.08682153505766621 1103 22-23 0.07281126339749022 1103 24-25 0.09612113596932659 1103 26-27 0.05773825413988476 1103 28-29 0.11633221220728984 1103 30-31 0.13570899677313264 1103 32-33 0.1216799871356713 1103 34-35 0.07552016492410019 1103 36-37 0.002985764892279974 1103 38-39 0.022815032986507333 1103 40-41 1.25964132448496 1103 42-43 0.46273943339627266 1103 44-45 0.20569738630855738 1103 46-47 0.6556136297936987 1103 48-49 0.2932521115305349 1103 50-51 0.12552456639366483 1103 52-53 0.1800572323553169 1103 54-55 0.15861696820115867 1103 56-57 0.11847761941282897 1103 58-59 0.16265755444182872 1103 60-61 0.1528790419153765 1103 62-63 0.11298145560038364 1103 64-65 0.142515865505203 1103 66-67 0.12086209987909058 1103 68-69 0.1678648657069033 1103 70-71 0.18652486727384598 1103 72-73 0.19196613548268004 1103 74-75 0.13227215198477893 1103 76 0.3361637473842265 2116 1 0.014922080789439462 2116 2 0.014601382551035158 2116 3 -0.005939139671873761 2116 4 -0.024923718963684394 2116 5 0.029559600607768743 2116 6 0.011014751886449403 2116 7 0.06815970682372097 2116 8 0.09001482352491053 2116 9 0.08989232436293548 2116 10-11 0.03728017994205146 2116 12-13 0.11393504207511285 2116 14-15 0.07509870531370666 2116 16-17 0.08864818347534253 2116 18-19 0.08181965864579155 2116 20-21 0.1275078131810119 2116 22-23 0.07522933117319042 2116 24-25 0.14651297271717567 2116 26-27 0.10641083070463964 2116 28-29 0.026846887973000833 2116 30-31 0.08778095495019045 2116 32-33 0.13632425064753306 2116 34-35 0.06228572586145731 2116 36-37 0.0472881736188171 2116 38-39 0.057552980715186663 2116 40-41 1.2613416948633542 2116 42-43 0.41816870881561385 2116 44-45 0.19202577496674422 2116 46-47 0.6152087406515108 2116 48-49 0.28102528229483426 2116 50-51 0.1469260240415693 2116 52-53 0.14675070565134973 2116 54-55 0.10925422267401075 2116 56-57 0.09233228678409944 2116 58-59 0.16433529160909188 2116 60-61 0.16402955541858688 2116 62-63 0.12427231804897332 2116 64-65 0.20838098631011093 2116 66-67 0.1916723757485599 2116 68-69 0.13328915145343956 2116 70-71 0.17334208721981526 2116 72-73 0.1445535895194041 2116 74-75 0.11242236509402659 2116 76 0.2734698677270302 1117 1 0.0023246047097558176 1117 2 0.03290689191577201 1117 3 0.016672775601286105 1117 4 -0.002051713828727486 1117 5 0.05111732799625912 1117 6 0.029589915782246123 1117 7 0.056609043462046316 1117 8 0.08694917417029302 1117 9 0.12019227469580329 1117 10-11 0.1164873380022371 1117 12-13 0.0813418182399559 1117 14-15 0.061325581573392185 1117 16-17 0.15178934762235485 1117 18-19 0.11509110972135517 1117 20-21 0.11242519402885875 1117 22-23 0.13331792997219338 1117 24-25 0.08898592371726011 1117 26-27 0.12970829076638068 1117 28-29 0.16456060935534111 1117 30-31 0.18407942794497956 1117 32-33 0.14927947992877932 1117 34-35 0.10767451814334805 1117 36-37 0.18470694595318093 1117 38-39 0.10097231540601115 1117 40-41 1.3306042473393944 1117 42-43 0.4775833971514416 1117 44-45 0.27076207473039915 1117 46-47 0.6265649416109724 1117 48-49 0.3101159933876545 1117 50-51 0.15972470388673798 1117 52-53 0.1481089463053351 1117 54-55 0.1736554455688406 1117 56-57 0.1410523496574001 1117 58-59 0.10311236221593845 1117 60-61 0.21587225233266594 1117 62-63 0.14238157883831803 1117 64-65 0.22945258650232603 1117 66-67 0.21557252521092352 1117 68-69 0.20240183823817404 1117 70-71 0.2588094365503508 1117 72-73 0.13765769345394574 1117 74-75 0.1707318731819356 1117 76 0.3396682113106877 2117 1 0.002569306907176383 2117 2 -0.011208883890958532 2117 3 0.006181960570238232 2117 4 8.90470239141905E-4 2117 5 0.016915157919591195 2117 6 -0.03798358843958738 2117 7 0.037352419949336024 2117 8 -0.001742417574320143 2117 9 0.060091975583347335 2117 10-11 -0.030895643967518538 2117 12-13 -0.08295094922674195 2117 14-15 -0.05156101191293061 2117 16-17 0.07485454492429255 2117 18-19 0.11616017043916571 2117 20-21 0.04454912273703826 2117 22-23 0.0501449485494021 2117 24-25 0.049509269746323525 2117 26-27 0.07007983506755977 2117 28-29 0.10094048504662823 2117 30-31 0.061211143940724355 2117 32-33 0.06977472838788401 2117 34-35 0.05146080963836397 2117 36-37 0.14313477751746007 2117 38-39 0.0620000358467081 2117 40-41 1.2721752642353579 2117 42-43 0.49566804108095397 2117 44-45 0.2692914145238703 2117 46-47 0.7691873143010568 2117 48-49 0.3289972149410261 2117 50-51 0.1694983975368558 2117 52-53 0.16534289024262705 2117 54-55 0.22175900353879285 2117 56-57 0.23209783259877526 2117 58-59 0.28881311733812964 2117 60-61 0.27482043592264205 2117 62-63 0.23449281182072212 2117 64-65 -0.10890275563453145 2117 66-67 0.09158206571777328 2117 68-69 0.1971126722120573 2117 70-71 0.297447625638938 2117 72-73 0.19073403190299132 2117 74-75 0.21914361197817556 2117 76 0.4316373730761143 1116 1 -0.028655182888741138 1116 2 0.0030148055848329136 1116 3 0.01456931551221885 1116 4 0.017585148110292437 1116 5 0.04319166852383205 1116 6 0.032842742092626054 1116 7 0.08594785812460515 1116 8 0.07323557362216349 1116 9 0.10683667346250303 1116 10-11 0.03829703310330501 1116 12-13 -0.017425739142709062 1116 14-15 0.06597700329315614 1116 16-17 0.0853847570091304 1116 18-19 0.08634924606941752 1116 20-21 0.09462115154856576 1116 22-23 0.09081621705680476 1116 24-25 0.0623243957967361 1116 26-27 0.11818807836487366 1116 28-29 0.18583844423029205 1116 30-31 0.109788574913118 1116 32-33 0.1118565611209732 1116 34-35 0.10369370279562418 1116 36-37 0.18539468147904614 1116 38-39 0.16023833116483388 1116 40-41 1.3148043158560085 1116 42-43 0.41997976577083307 1116 44-45 0.18106012200686195 1116 46-47 0.6002237288668866 1116 48-49 0.29741558228796805 1116 50-51 0.12145217904947003 1116 52-53 0.11294793225943778 1116 54-55 0.19720118040953594 1116 56-57 0.1559983928228661 1116 58-59 0.20319286923889734 1116 60-61 0.10441627425797861 1116 62-63 0.15015307592636162 1116 64-65 -0.09590455635481732 1116 66-67 0.02998242586183153 1116 68-69 0.16518189990313203 1116 70-71 0.21129316385422214 1116 72-73 0.2182820511106769 1116 74-75 0.1372058368681266 1116 76 0.4150707464254566 2118 1 0.031103647472676244 2118 2 -0.03720757605074709 2118 3 -0.012340570393362782 2118 4 0.04548294137394038 2118 5 0.01670434188586256 2118 6 0.07346001339139008 2118 7 0.07260359358951973 2118 8 0.0702971299520101 2118 9 0.06475530432948062 2118 10-11 0.06597090753112411 2118 12-13 0.0339411734759949 2118 14-15 0.08452366586062254 2118 16-17 0.10777698219188636 2118 18-19 0.12614172403621637 2118 20-21 0.10467512435720039 2118 22-23 0.09754488413339146 2118 24-25 0.08088181876593126 2118 26-27 0.13292350912254847 2118 28-29 0.19283577974987054 2118 30-31 0.1584197834941108 2118 32-33 0.16413588748567065 2118 34-35 0.161503363245167 2118 36-37 0.22790527108073633 2118 38-39 0.197831929579813 2118 40-41 1.296806092176297 2118 42-43 0.5161835643634376 2118 44-45 0.23950925775872633 2118 46-47 0.6501533592556257 2118 48-49 0.2797150630558747 2118 50-51 0.12981130318692635 2118 52-53 0.17972766854419575 2118 54-55 0.16541939452444865 2118 56-57 0.2179077663283664 2118 58-59 0.0763393473423335 2118 60-61 0.1624657379458938 2118 62-63 0.17144808173356552 2118 64-65 0.18445019530194173 2118 66-67 0.1853737287244357 2118 68-69 0.1409409352245774 2118 70-71 0.1750152380497525 2118 72-73 0.14278656023140002 2118 74-75 0.17737373329499206 2118 76 0.4174482828058643 1119 1 0.002581937524766431 1119 2 0.03876304013216725 1119 3 -0.015866068353304286 1119 4 0.008094437281805256 1119 5 0.013698293930481498 1119 6 -0.026836982689459887 1119 7 0.01887131172408374 1119 8 0.026411914856105057 1119 9 0.0033558128964230605 1119 10-11 0.025287800607635802 1119 12-13 0.09348943411535515 1119 14-15 0.11461539437917878 1119 16-17 0.08106509389036631 1119 18-19 0.09595129660160495 1119 20-21 0.041980963838156526 1119 22-23 0.08387158369286851 1119 24-25 0.0358808619442641 1119 26-27 0.08792909807674221 1119 28-29 0.10713677455981241 1119 30-31 0.13405031531506495 1119 32-33 0.11101506646550519 1119 34-35 0.08963276492368522 1119 36-37 0.10539680682335018 1119 38-39 0.17128442563213753 1119 40-41 1.2491026121800104 1119 42-43 0.44618616787463594 1119 44-45 0.19560779206274503 1119 46-47 0.5797814305478894 1119 48-49 0.2098375396450578 1119 50-51 0.06210840035731735 1119 52-53 0.11775138041803501 1119 54-55 0.11724550092279173 1119 56-57 0.04891535703772831 1119 58-59 0.06777539494138551 1119 60-61 0.09744355801127824 1119 62-63 0.12138934620214314 1119 64-65 0.125227402022432 1119 66-67 0.13135582335223006 1119 68-69 0.12013145839827644 1119 70-71 0.19624005858450033 1119 72-73 0.04170666089746078 1119 74-75 0.1381119419904948 1119 76 0.23395060496253706 2119 1 -0.03089621200180659 2119 2 0.05243818631153374 2119 3 0.023768141962229095 2119 4 -0.006445544735562692 2119 5 0.01522168432162374 2119 6 0.0017312548436976272 2119 7 0.021784012491565363 2119 8 0.07723467089140001 2119 9 0.07497366285541318 2119 10-11 0.028572930191991475 2119 12-13 0.057596264919581586 2119 14-15 0.06517075188263988 2119 16-17 0.023551085151758855 2119 18-19 0.06390002467469458 2119 20-21 0.03257646637591449 2119 22-23 0.05231192908568971 2119 24-25 0.06391545876221016 2119 26-27 0.07924032390764779 2119 28-29 0.12078766562501642 2119 30-31 0.10480550111488185 2119 32-33 0.14332519122704213 2119 34-35 0.0917505748335401 2119 36-37 0.15354126238629107 2119 38-39 0.09153995608020438 2119 40-41 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-0.16864937704405492 2104 46-47 0.15577543308409503 2104 48-49 -0.06978607653847746 2104 50-51 -0.18473313909859002 2104 52-53 -0.15441156266505374 2104 54-55 -0.31829688016651403 2104 56-57 -0.11169431164194066 2104 58-59 -0.19490113092616212 2104 60-61 -0.19440191506280513 2104 62-63 -0.08971694140466013 2104 64-65 -0.0754867549188134 2104 66-67 -0.2677428774310613 2104 68-69 -0.3261868382333688 2104 70-71 -0.10541064071786366 2104 72-73 -0.1072422123208625 2104 74-75 -0.1597375071939311 2104 76 -0.3584870649139873 2111 1 -0.1053223207978391 2111 2 -0.04700416192299883 2111 3 0.004853710319125071 2111 4 -0.009496683137790285 2111 5 -0.0027105251096770644 2111 6 0.020941396695484116 2111 7 0.005194341477967157 2111 8 -0.01667583280087115 2111 9 -0.10364104855158729 2111 10-11 -0.03629917103891245 2111 12-13 0.039146855753742216 2111 14-15 0.012879232269860097 2111 16-17 -0.04123301825536174 2111 18-19 -0.026945622100512878 2111 20-21 0.03941227334117059 2111 22-23 0.006589584790837932 2111 24-25 -0.03858922546571364 2111 26-27 0.058846227324615086 2111 28-29 0.03810535594302422 2111 30-31 -0.017975968806396736 2111 32-33 -0.0199967764535387 2111 34-35 0.009200877461630341 2111 36-37 0.05952364875125937 2111 38-39 0.06735521106870124 2111 40-41 1.1348269354926828 2111 42-43 -0.10938912280389701 2111 44-45 -0.05573660757067245 2111 46-47 0.39939222159797083 2111 48-49 -0.06549227803577651 2111 50-51 -0.3300797711873287 2111 52-53 -0.21432251377152767 2111 54-55 -0.15306554280621754 2111 56-57 -0.2696023823709339 2111 58-59 -0.14541378470422472 2111 60-61 -0.21344711256137572 2111 62-63 -0.2958747038149241 2111 64-65 -0.2313836331909016 2111 66-67 -0.0965399817157433 2111 68-69 -0.12289554879035336 2111 70-71 -0.06230430086149852 2111 72-73 -0.3578367373250302 2111 74-75 -0.5307641096458582 2111 76 -0.020371396785115792 2110 1 0.027149628921435465 2110 2 0.016834368506273734 2110 3 -0.009723246260236351 2110 4 3.8704683712609267E-4 2110 5 -0.04809235129906142 2110 6 0.0670393810211678 2110 7 0.05584584217808697 2110 8 -0.017307815071362143 2110 9 -0.09103311983624707 2110 10-11 -0.07217744550120386 2110 12-13 -0.17574900434934904 2110 14-15 -0.0729453589737048 2110 16-17 -0.12830040947764587 2110 18-19 -0.11808818647122621 2110 20-21 -0.22728511912446692 2110 22-23 -0.1503689494299678 2110 24-25 -0.13225013155258125 2110 26-27 -0.035371831341144855 2110 28-29 -0.08903499332410547 2110 30-31 -0.030495711845702544 2110 32-33 -0.09947501391703639 2110 34-35 -0.044907320845616994 2110 36-37 -0.05287148265173158 2110 38-39 -0.05759017411004663 2110 40-41 0.9408699124577993 2110 42-43 0.009906590509991986 2110 44-45 -0.2276973492975216 2110 46-47 0.06285478860250038 2110 48-49 -0.1705859671009904 2110 50-51 -0.3451791186073052 2110 52-53 -0.38373515676949665 2110 54-55 -0.3517387357957915 2110 56-57 -0.38335919105884386 2110 58-59 -0.47808557974732935 2110 60-61 -0.2038067449033818 2110 62-63 -0.4097704991580713 2110 64-65 -0.30797124971508083 2110 66-67 -0.6004965790474657 2110 68-69 -0.6008625159134056 2110 70-71 -0.3761805777750169 2110 72-73 -0.6950882180303708 2110 74-75 -0.3156908531264193 2110 76 -0.37900327606679696 2109 1 -0.02476464346074181 2109 2 -0.057689696210871944 2109 3 -0.02638759945121194 2109 4 -0.07639392384923127 2109 5 -0.01232115198898498 2109 6 0.04294935101030717 2109 7 -0.08673782752525483 2109 8 -0.032353549523968184 2109 9 -0.10335453055606791 2109 10-11 -0.15094796457002957 2109 12-13 -0.14120934153380205 2109 14-15 -0.06981480804425644 2109 16-17 -0.17515067390089456 2109 18-19 -0.17278049227041947 2109 20-21 -0.19476532516690526 2109 22-23 -0.2530949342240518 2109 24-25 -0.19358622452756435 2109 26-27 -0.2197831008359401 2109 28-29 -0.3277485550895989 2109 30-31 -0.2420095780739615 2109 32-33 -0.39419708935450615 2109 34-35 -0.12102942424020569 2109 36-37 -0.4709937102595134 2109 38-39 -0.18856406350889188 2109 40-41 -10.764452225648093 2109 42-43 -0.9254115909723168 2109 44-45 -0.32983570312349286 2109 46-47 0.07504755867442015 2109 48-49 -0.20614000707381308 2109 50-51 -0.22936922267584237 2109 52-53 -0.11317853085400031 2109 54-55 -0.3539623900048454 2109 56-57 -0.2415538079622337 2109 58-59 -0.21530846113540036 2109 60-61 -0.3648626986691994 2109 62-63 -0.1451830374716394 2109 64-65 -0.31962032755430414 2109 66-67 -0.26562152230726355 2109 68-69 -0.47683937415397537 2109 70-71 -0.39149763926231174 2109 72-73 -0.2042231391388114 2109 74-75 -0.36218490539720705 2109 76 -0.18475027945419953 2108 1 0.030241835585741228 2108 2 -0.06986825032639388 2108 3 -0.018101863074456048 2108 4 -0.006796292045791574 2108 5 -0.10175284705448462 2108 6 -0.0499269060995573 2108 7 -0.13617030540201114 2108 8 -0.12847346850941932 2108 9 -0.10255818050981702 2108 10-11 -0.19223387964591865 2108 12-13 -0.2796842904505539 2108 14-15 -0.2119525436856904 2108 16-17 -0.420674934473098 2108 18-19 -0.49400240931744577 2108 20-21 -0.49405453895811036 2108 22-23 -0.19498419145486423 2108 24-25 -0.5963595259153465 2108 26-27 -0.5378162811253944 2108 28-29 -0.9700935111382805 2108 30-31 -0.510686091265768 2108 32-33 -0.8619308603777114 2108 34-35 -0.483207705655083 2108 36-37 -1.0141018431231075 2108 38-39 -0.568537505674378 2108 40-41 -7.744019536348127 2108 42-43 -0.6541465070174084 2108 44-45 -0.31454929205325044 2108 46-47 0.298953074638483 2108 48-49 -0.0073141598009272 2108 50-51 -0.1340028236942743 2108 52-53 -0.35654996421029495 2108 54-55 -0.3949438107495382 2108 56-57 -0.3510513297550304 2108 58-59 -0.255142902970114 2108 60-61 -0.4439185833726569 2108 62-63 -0.37295923429862654 2108 64-65 -0.2533259026141934 2108 66-67 -0.2697729244216873 2108 68-69 -0.22296485944081468 2108 70-71 -0.20657160048941847 2108 72-73 -0.3042536174461219 2108 74-75 -0.37173950183073146 2108 76 -0.765137777445041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 5.0 17 13.0 18 30.0 19 81.0 20 133.0 21 255.0 22 365.0 23 576.0 24 748.0 25 1065.0 26 1583.0 27 2385.0 28 3658.0 29 5242.0 30 7605.0 31 11323.0 32 16690.0 33 24975.0 34 37996.0 35 62184.0 36 138293.0 37 1242853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83520531943571 26.46740176886641 8.628294160687009 24.069098751010873 2 15.093963005275793 23.607178157452218 41.121651284289435 20.177207552982555 3 18.159955329063067 26.976368047443618 32.677560556076145 22.18611606741717 4 13.423295636881763 16.884587243110023 41.89748790162125 27.79462921838697 5 14.377238360525268 33.277922544703024 35.57070972876526 16.774129366006445 6 25.912545088122407 38.9711564381346 19.47460303197566 15.641695441767325 7 22.37551827272377 35.455053078828804 24.381153485745095 17.788275162702334 8 19.596998831880672 41.336405529953915 23.03653261106761 16.030063027097803 9 20.828016892802587 18.439405414425632 25.284969770098716 35.44760792267307 10-11 22.814975033053926 26.911447569413244 31.14790187797646 19.12567551955637 12-13 20.519748918526886 25.47257486874703 31.62705544074041 22.380620771985672 14-15 19.84827285213663 28.996925663966728 26.046429534164282 25.108371949732362 16-17 19.153530672759715 33.766414643851974 27.872674993260848 19.207379690127464 18-19 18.820648755503637 32.15043066377418 30.485122524164666 18.543798056557513 20-21 19.0316162407096 30.173131971811095 30.8742923892533 19.920959398226 22-23 19.218130238886822 30.434322169877927 30.79409650462755 19.553451086607705 24-25 19.124584419085274 31.069599373579965 30.584797761318562 19.2210184460162 26-27 18.851937666071912 31.273314249772156 30.481432037277127 19.393316046878812 28-29 18.67315764476336 31.296837092281425 30.836617331810075 19.193387931145143 30-31 18.85697598295316 31.043380871083272 30.93796131086094 19.161681835102627 32-33 18.939033156617846 30.91469519787428 30.84303556987536 19.30323607563252 34-35 18.93117081498787 30.776382167567363 31.19684094322427 19.095606074220505 36-37 19.14338985661656 31.014915985263723 31.086607704452973 18.75508645366674 38-39 18.96184999293994 30.800097557218592 30.872270644262738 19.365781805578735 40-41 19.048817118724568 30.667785579502716 30.89951606484988 19.38388123692284 42-43 19.271529979590003 30.687938847027713 30.76989974712142 19.270631426260863 44-45 18.888393258282736 30.34215627126041 31.117094335263083 19.652356135193767 46-47 18.864838324583136 30.33455065915305 31.42083745170276 19.379773564561056 48-49 18.86531969243803 30.475174255163473 31.252422884869645 19.40708316752885 50-51 18.61353221313685 30.37338099944803 31.45514293416171 19.557943853253406 52-53 18.287774540133242 30.417025018291977 31.68372848285689 19.611471958717893 54-55 18.267717546179224 30.292928385299668 31.773327086248283 19.66602698227283 56-57 18.079021347059804 30.151502509531085 31.94899426209517 19.820481881313942 58-59 17.84417801625098 30.2881147067507 32.04732166925536 19.820385607742963 60-61 18.243681244624728 29.990083822189135 31.816521828427657 19.94971310475848 62-63 18.101677727430264 29.491900183561608 31.82775374504191 20.578668343966214 64-65 17.99192585651387 29.304327176103616 31.859042655610182 20.844704311772333 66-67 18.004280964789547 29.478582339576135 31.34526269848401 21.171873997150303 68-69 18.00514742692836 29.445817234252853 31.063758776940553 21.485276561878234 70-71 18.04715479506566 29.341264136169336 30.99347907012567 21.618101998639332 72-73 17.87825886037765 29.453807940644133 30.81826117094335 21.849672028034863 74-75 18.10049035338819 29.26366763796003 30.382815809404 22.253026199247785 76 18.038843176771113 29.05446516822202 30.470200120662877 22.43649153434399 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 1.5 2 2.0 3 2.5 4 1.0 5 3.0 6 4.5 7 7.0 8 10.0 9 23.0 10 56.0 11 143.0 12 210.0 13 333.0 14 632.5 15 1213.0 16 2124.0 17 2631.0 18 3678.5 19 6150.0 20 9336.0 21 11098.0 22 13407.5 23 18826.0 24 25327.5 25 28720.0 26 32195.5 27 39574.5 28 47189.0 29 50900.0 30 54298.0 31 61041.5 32 67156.5 33 69926.0 34 71962.0 35 76732.0 36 81181.0 37 82896.0 38 83732.0 39 85387.0 40 86313.0 41 85816.5 42 85213.0 43 84287.5 44 81035.5 45 75297.0 46 71885.0 47 67666.0 48 58700.5 49 50060.0 50 46166.0 51 42943.0 52 35488.0 53 27787.0 54 24318.0 55 21516.0 56 16427.5 57 12263.5 58 10386.0 59 9190.5 60 6847.0 61 4785.5 62 3872.0 63 3358.5 64 2370.5 65 1576.5 66 1014.5 67 772.0 68 653.0 69 413.0 70 225.5 71 159.0 72 133.0 73 82.0 74 40.5 75 24.0 76 18.0 77 10.5 78 7.0 79 5.0 80 3.5 81 2.0 82 3.5 83 5.0 84 2.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1558060.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.60200056548024 #Duplication Level Percentage of deduplicated Percentage of total 1 95.77008421275119 87.72731308212522 2 3.1292103027443265 5.732838478429848 3 0.48756422958827433 1.339855765033591 4 0.17474139049774168 0.6402664380474774 5 0.09343505446858841 0.4279418956133656 6 0.08265450835998159 0.4542790992918315 7 0.04735238988509528 0.3036301551521937 8 0.031154706346504607 0.2283066742695908 9 0.026221590884459956 0.2161755164723483 >10 0.1515670359568904 2.3565869250675484 >50 0.004534229172467366 0.28146591178853286 >100 0.0014060420187472853 0.25390321037928576 >500 7.430732575920782E-5 0.03743684832917853 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.567295226114527E-4 0.0 0.0 2 3.8509428391717904E-4 0.0 0.0 3 3.8509428391717904E-4 0.0 0.0 4 3.8509428391717904E-4 0.0 0.0 5 3.8509428391717904E-4 0.0 0.0 6 3.8509428391717904E-4 0.0 0.0 7 3.8509428391717904E-4 0.0 0.0 8 3.8509428391717904E-4 0.0 0.0 9 3.8509428391717904E-4 0.0 0.0 10 4.4927666457004225E-4 0.0 6.418238065286317E-5 11 4.4927666457004225E-4 6.418238065286317E-5 6.418238065286317E-5 12 4.4927666457004225E-4 1.2836476130572634E-4 6.418238065286317E-5 13 4.4927666457004225E-4 1.2836476130572634E-4 6.418238065286317E-5 14 4.4927666457004225E-4 1.2836476130572634E-4 6.418238065286317E-5 15 4.4927666457004225E-4 1.2836476130572634E-4 6.418238065286317E-5 16 4.4927666457004225E-4 1.2836476130572634E-4 6.418238065286317E-5 17 5.134590452229054E-4 1.2836476130572634E-4 6.418238065286317E-5 18 5.776414258757686E-4 1.2836476130572634E-4 6.418238065286317E-5 19 5.776414258757686E-4 1.2836476130572634E-4 6.418238065286317E-5 20 5.776414258757686E-4 1.2836476130572634E-4 1.2836476130572634E-4 21 5.776414258757686E-4 1.2836476130572634E-4 2.567295226114527E-4 22 6.418238065286318E-4 1.9254714195858952E-4 5.134590452229054E-4 23 6.418238065286318E-4 1.9254714195858952E-4 5.134590452229054E-4 24 6.418238065286318E-4 1.9254714195858952E-4 7.060061871814949E-4 25 6.418238065286318E-4 1.9254714195858952E-4 8.343709484872212E-4 26 6.418238065286318E-4 1.9254714195858952E-4 0.0011552828517515372 27 6.418238065286318E-4 1.9254714195858952E-4 0.003594213316560338 28 6.418238065286318E-4 1.9254714195858952E-4 0.010397545665763834 29 6.418238065286318E-4 1.9254714195858952E-4 0.016623236589091563 30 7.060061871814949E-4 1.9254714195858952E-4 0.02201455656393207 31 7.060061871814949E-4 1.9254714195858952E-4 0.027405876538772578 32 7.060061871814949E-4 1.9254714195858952E-4 0.03369574984275317 33 7.701885678343581E-4 1.9254714195858952E-4 0.04114090599848529 34 7.701885678343581E-4 2.567295226114527E-4 0.05500430021950374 35 7.701885678343581E-4 2.567295226114527E-4 0.07368137298948693 36 8.343709484872212E-4 2.567295226114527E-4 0.11283262518773346 37 8.343709484872212E-4 2.567295226114527E-4 0.18362579104784155 38 8.343709484872212E-4 2.567295226114527E-4 0.2858041410471997 39 8.343709484872212E-4 2.567295226114527E-4 0.4087775823780856 40 8.985533291400845E-4 2.567295226114527E-4 0.5640347611773616 41 8.985533291400845E-4 3.209119032643159E-4 0.7258385428032297 42 9.627357097929477E-4 3.209119032643159E-4 0.9183856847618191 43 9.627357097929477E-4 3.8509428391717904E-4 1.121137825244214 44 0.0010269180904458107 3.8509428391717904E-4 1.3399997432704773 45 0.0010269180904458107 3.8509428391717904E-4 1.6099508362964199 46 0.001091100471098674 5.134590452229054E-4 1.934007676212726 47 0.0011552828517515372 5.134590452229054E-4 2.2765490417570566 48 0.0011552828517515372 5.134590452229054E-4 2.6450200890851443 49 0.0011552828517515372 5.134590452229054E-4 3.030820379189505 50 0.0011552828517515372 5.134590452229054E-4 3.454231544356443 51 0.0011552828517515372 5.134590452229054E-4 3.865191327676726 52 0.0011552828517515372 5.134590452229054E-4 4.2852650090497155 53 0.0012194652324044003 5.134590452229054E-4 4.704119225190301 54 0.0012836476130572635 5.134590452229054E-4 5.150250953108353 55 0.0012836476130572635 5.134590452229054E-4 5.617241954738585 56 0.0012836476130572635 5.134590452229054E-4 6.113307574804565 57 0.0013478299937101266 5.134590452229054E-4 6.624776966227231 58 0.0013478299937101266 5.134590452229054E-4 7.169043554163511 59 0.0013478299937101266 5.134590452229054E-4 7.72807208964995 60 0.0013478299937101266 5.134590452229054E-4 8.330487914457724 61 0.0013478299937101266 5.134590452229054E-4 8.911081729843524 62 0.0014761947550158531 5.776414258757686E-4 9.479994351950502 63 0.0014761947550158531 5.776414258757686E-4 10.04460675455374 64 0.0014761947550158531 5.776414258757686E-4 10.640861070818838 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACTC 535 0.0 18.971962 70 GCTACAC 300 1.8189894E-12 18.666666 3 TAGGGCG 100 0.009691875 17.5 5 GACGGAC 975 0.0 17.23077 70 ACAGCGC 185 4.7519497E-6 17.027027 8 ACAGACC 235 1.543849E-7 16.382978 8 GCCCTAT 130 0.0022912887 16.153847 1 ACGGACT 720 0.0 16.041666 70 TAGGGCC 175 5.6229175E-5 16.0 5 GGTATAT 430 0.0 15.4651165 1 CGCGTAA 160 5.163585E-4 15.3125 10 TATACAG 530 0.0 15.18868 5 CTAGGGC 300 9.746145E-9 15.166667 4 GTATAGG 280 6.8765075E-8 15.000001 1 TGGACAG 445 0.0 14.94382 5 TACACAG 445 0.0 14.94382 5 CATACCG 235 2.7725546E-6 14.893618 5 TTAGTAC 400 9.094947E-12 14.874999 3 ACTGCTC 660 0.0 14.848485 8 ACACCAC 710 0.0 14.788733 6 >>END_MODULE