##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve11.3410000000c7cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1493717 Sequences flagged as poor quality 0 Sequence length 76 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.749121821603424 34.0 34.0 34.0 34.0 34.0 2 33.77714051590763 34.0 34.0 34.0 34.0 34.0 3 33.79484935901513 34.0 34.0 34.0 34.0 34.0 4 33.81361663554743 34.0 34.0 34.0 34.0 34.0 5 33.82159271133688 34.0 34.0 34.0 34.0 34.0 6 37.61105082154116 38.0 38.0 38.0 37.0 38.0 7 37.64098487196705 38.0 38.0 38.0 37.0 38.0 8 37.555530264434296 38.0 38.0 38.0 37.0 38.0 9 37.52935663181178 38.0 38.0 38.0 37.0 38.0 10-11 37.52384420877583 38.0 38.0 38.0 37.0 38.0 12-13 37.461236298442074 38.0 38.0 38.0 37.0 38.0 14-15 37.51623366407425 38.0 38.0 38.0 37.0 38.0 16-17 37.46302211195294 38.0 38.0 38.0 37.0 38.0 18-19 37.48006081473264 38.0 38.0 38.0 37.0 38.0 20-21 37.42814970975091 38.0 38.0 38.0 37.0 38.0 22-23 37.48217768158226 38.0 38.0 38.0 37.0 38.0 24-25 37.41607345969819 38.0 38.0 38.0 37.0 38.0 26-27 37.3826608386997 38.0 38.0 38.0 37.0 38.0 28-29 37.30725398452317 38.0 38.0 38.0 37.0 38.0 30-31 37.33099609899332 38.0 38.0 38.0 37.0 38.0 32-33 37.287901590461914 38.0 38.0 38.0 37.0 38.0 34-35 37.33280065768817 38.0 38.0 38.0 37.0 38.0 36-37 37.232266553838514 38.0 38.0 38.0 37.0 38.0 38-39 37.29641993764548 38.0 38.0 38.0 37.0 38.0 40-41 36.08253973142168 38.0 37.5 38.0 33.0 38.0 42-43 36.797428495491445 38.0 37.0 38.0 35.0 38.0 44-45 37.00698191156692 38.0 38.0 38.0 36.0 38.0 46-47 36.56531156838946 38.0 37.5 38.0 35.0 38.0 48-49 36.911416620417384 38.0 37.5 38.0 35.0 38.0 50-51 36.97485969564516 38.0 38.0 38.0 36.0 38.0 52-53 36.994033675723045 38.0 38.0 38.0 36.0 38.0 54-55 36.97862212186111 38.0 38.0 38.0 36.0 38.0 56-57 36.97072705204533 38.0 38.0 38.0 36.0 38.0 58-59 36.942173785261865 38.0 38.0 38.0 36.0 38.0 60-61 36.93814993067629 38.0 38.0 38.0 36.0 38.0 62-63 36.94403056268356 38.0 38.0 38.0 36.0 38.0 64-65 36.86552606685202 38.0 38.0 38.0 35.5 38.0 66-67 36.84199014940581 38.0 38.0 38.0 36.0 38.0 68-69 36.82687617533978 38.0 38.0 38.0 35.0 38.0 70-71 36.73709477765868 38.0 38.0 38.0 34.5 38.0 72-73 36.794918314513396 38.0 38.0 38.0 35.0 38.0 74-75 36.78980958240416 38.0 38.0 38.0 35.0 38.0 76 36.043619373683235 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.008184788318459368 1101 2 0.034569210418403884 1101 3 0.02373342738732731 1101 4 -0.0403368730583864 1101 5 -0.0033900180945636293 1101 6 0.056637972368662304 1101 7 0.020232183361748923 1101 8 0.09441588544038382 1101 9 0.060214683340689135 1101 10-11 0.04257669459430247 1101 12-13 0.08110047576617063 1101 14-15 0.08791265291758776 1101 16-17 0.10486947875623542 1101 18-19 0.05893705742562361 1101 20-21 0.10572848039246452 1101 22-23 0.033992115055617944 1101 24-25 0.06283670859309609 1101 26-27 0.06997440161511292 1101 28-29 0.1417133643950521 1101 30-31 0.10906546025546504 1101 32-33 0.1608792699610504 1101 34-35 0.08288470211954291 1101 36-37 0.08700823494129395 1101 38-39 0.11026547338094161 1101 40-41 1.369738983921664 1101 42-43 0.5072945047997948 1101 44-45 0.23947931188866534 1101 46-47 0.7427787943993991 1101 48-49 0.31355457123309094 1101 50-51 0.21030075226058642 1101 52-53 0.28163975610777925 1101 54-55 0.20579185418746704 1101 56-57 0.18014912242461634 1101 58-59 0.2253216204280193 1101 60-61 0.272289474820127 1101 62-63 0.19972163298998424 1101 64-65 0.17691423394843042 1101 66-67 0.15365999367129746 1101 68-69 0.22411469928837136 1101 70-71 0.253101195517047 1101 72-73 0.21045648100297143 1101 74-75 0.27012356525252557 1101 76 0.45320970933344285 1102 1 -0.023094298702481808 1102 2 0.045306511158919704 1102 3 0.09500608538025546 1102 4 0.0663901951511292 1102 5 0.043491880969042995 1102 6 -0.02030981605178539 1102 7 0.03231237330227543 1102 8 0.030570282583973096 1102 9 0.048365702856955295 1102 10-11 0.0558719501382825 1102 12-13 0.12090960755509172 1102 14-15 0.0801360551107706 1102 16-17 0.13318201984662892 1102 18-19 0.14690816300542764 1102 20-21 0.09021360220034325 1102 22-23 0.05990014897329132 1102 24-25 0.11797850321978132 1102 26-27 0.14601568051065783 1102 28-29 0.18942584991925315 1102 30-31 0.15893807558911988 1102 32-33 0.17443966308118775 1102 34-35 0.14609403410808852 1102 36-37 0.0018318099069034588 1102 38-39 0.10366141239858706 1102 40-41 1.339641005207163 1102 42-43 0.6471154283896468 1102 44-45 0.35837714388183883 1102 46-47 0.8335305228947263 1102 48-49 0.4783436842452744 1102 50-51 0.3306495451906315 1102 52-53 0.322775880705116 1102 54-55 0.32956406553924467 1102 56-57 0.32516312429849137 1102 58-59 0.3651247495496648 1102 60-61 0.357640107698181 1102 62-63 0.3156738280325371 1102 64-65 0.3341561198488776 1102 66-67 0.378945494050555 1102 68-69 0.23108396207303628 1102 70-71 0.44713106839574124 1102 72-73 0.39051728114191064 1102 74-75 0.4476666854010105 1102 76 0.5988060648365447 1103 1 0.0014580838071083235 1103 2 0.01603172032902478 1103 3 0.03248474013711444 1103 4 0.07380904960567847 1103 5 0.008245730721888833 1103 6 0.04761326922902498 1103 7 0.08958185971759036 1103 8 0.11571307319160695 1103 9 0.13911868694406593 1103 10-11 0.1491963613811862 1103 12-13 0.1920705339660671 1103 14-15 0.0920593088531092 1103 16-17 0.1817432664052845 1103 18-19 0.17285371524955906 1103 20-21 0.20094148693585367 1103 22-23 0.16131964966890422 1103 24-25 0.18508962872326862 1103 26-27 0.15717988413534556 1103 28-29 0.20006493844331175 1103 30-31 0.21254936336068653 1103 32-33 0.23480631109675443 1103 34-35 0.16267935438494163 1103 36-37 0.1540589172774105 1103 38-39 0.15903207907091854 1103 40-41 1.4480921819781614 1103 42-43 0.6139058576515524 1103 44-45 0.3575888076482059 1103 46-47 0.8490572960673006 1103 48-49 0.43810084483335743 1103 50-51 0.2713677221538049 1103 52-53 0.27381790017657437 1103 54-55 0.2940423072822398 1103 56-57 0.28402648324434665 1103 58-59 0.2754862304680401 1103 60-61 0.32022368814971003 1103 62-63 0.30991685006242164 1103 64-65 0.39795791424725024 1103 66-67 0.3520894178804639 1103 68-69 0.42116448031165277 1103 70-71 0.34653255220202084 1103 72-73 0.4025423727074795 1103 74-75 0.41628701028401593 1103 76 0.4517587954527613 2116 1 -0.013780623562524852 2116 2 -0.011705637662807078 2116 3 0.022461322560658914 2116 4 0.020718692314076748 2116 5 0.040575493307621 2116 6 0.017365226558027302 2116 7 0.060344720373706195 2116 8 0.07825688624048865 2116 9 0.15177521550712214 2116 10-11 0.1416757995815061 2116 12-13 0.1634561025632948 2116 14-15 0.06673085980948912 2116 16-17 0.1492285940082141 2116 18-19 0.14674609838930053 2116 20-21 0.20340723666965488 2116 22-23 0.1145948990460326 2116 24-25 0.16433748332335796 2116 26-27 0.17452852501129712 2116 28-29 0.09822755118614879 2116 30-31 0.13714834777774598 2116 32-33 0.17424124278470288 2116 34-35 0.12453454899757332 2116 36-37 0.13360107952098588 2116 38-39 0.1241380112104622 2116 40-41 1.4193241268305528 2116 42-43 0.5166928996304776 2116 44-45 0.33392590588592697 2116 46-47 0.7768354647978128 2116 48-49 0.4368134483085484 2116 50-51 0.32380990541428645 2116 52-53 0.27891646546670046 2116 54-55 0.27189008824929317 2116 56-57 0.2551330770295479 2116 58-59 0.2688241890578098 2116 60-61 0.27637066754941486 2116 62-63 0.21674006050680106 2116 64-65 0.3441208569266223 2116 66-67 0.3272355184932607 2116 68-69 0.3103540187473399 2116 70-71 0.36125921981288656 2116 72-73 0.31369301632892643 2116 74-75 0.2750910127970627 2116 76 0.5372973237857366 1117 1 -0.022165271563849842 1117 2 0.014873176669752297 1117 3 0.021651980146494054 1117 4 0.010520717979815686 1117 5 -0.00379674442226019 1117 6 0.026429672566955276 1117 7 0.09931460782661361 1117 8 0.0916268377744558 1117 9 0.1396819822854809 1117 10-11 0.11798125335967313 1117 12-13 0.09174408787190913 1117 14-15 0.11390413351654871 1117 16-17 0.1454598146473316 1117 18-19 0.1666561521576142 1117 20-21 0.11222194492773241 1117 22-23 0.17469857119578336 1117 24-25 0.09142380101207692 1117 26-27 0.19607982902667942 1117 28-29 0.19837391332756482 1117 30-31 0.1698878463221547 1117 32-33 0.18369083677847442 1117 34-35 0.12546619952563276 1117 36-37 0.2639244951361803 1117 38-39 0.14949823385907735 1117 40-41 1.377656401794546 1117 42-43 0.559800735513484 1117 44-45 0.3368415706460226 1117 46-47 0.7396128023595878 1117 48-49 0.35343889272029116 1117 50-51 0.27191650138070145 1117 52-53 0.23702550818611456 1117 54-55 0.2942215827569754 1117 56-57 0.23623876246359288 1117 58-59 0.2468006357226713 1117 60-61 0.3206460866370904 1117 62-63 0.25753745151907736 1117 64-65 0.34992876448732346 1117 66-67 0.26879291517170145 1117 68-69 0.2501320849004074 1117 70-71 0.31485768933403335 1117 72-73 0.23889202028425416 1117 74-75 0.24828751896138357 1117 76 0.5874579458540765 2117 1 -0.0010012195361497334 2117 2 0.014588895693485426 2117 3 0.038050992543908535 2117 4 0.07708687044870288 2117 5 0.039045160593609296 2117 6 0.013781907172564445 2117 7 0.08635363336666302 2117 8 0.05992510423192243 2117 9 0.10968998215215464 2117 10-11 0.042163112233460254 2117 12-13 -0.05740872386694207 2117 14-15 -0.04028776090425623 2117 16-17 0.1542435372995712 2117 18-19 0.14347064572558565 2117 20-21 0.10065892335904891 2117 22-23 0.07403584414599607 2117 24-25 0.07114086525148622 2117 26-27 0.14436366715318627 2117 28-29 0.174154733647228 2117 30-31 0.2039455525032423 2117 32-33 0.17944156236161746 2117 34-35 0.12230464507534577 2117 36-37 0.24099743677763286 2117 38-39 0.1691874890381584 2117 40-41 1.309710629462181 2117 42-43 0.6392257212875379 2117 44-45 0.3967782502727957 2117 46-47 0.8502546488049916 2117 48-49 0.4612533458127359 2117 50-51 0.24751172955546252 2117 52-53 0.1993136367172994 2117 54-55 0.3277561428952538 2117 56-57 0.27205858213326195 2117 58-59 0.3594705436051555 2117 60-61 0.3399066584847006 2117 62-63 0.25115779594191423 2117 64-65 -0.17634359525906262 2117 66-67 0.228673524304412 2117 68-69 0.34046694836696645 2117 70-71 0.40710763993010346 2117 72-73 0.28277813408711694 2117 74-75 0.3428894541672207 2117 76 0.5346953728493418 1116 1 0.028940123599227263 1116 2 0.017936658550773643 1116 3 0.03796796006169956 1116 4 0.03760456098032705 1116 5 0.05721300760018977 1116 6 -0.001681921498892791 1116 7 0.06502423962517412 1116 8 0.03829526400875238 1116 9 0.11863017691430855 1116 10-11 0.03159686469469136 1116 12-13 -0.06324837163440833 1116 14-15 0.06344499832339778 1116 16-17 0.1154872157842064 1116 18-19 0.10779599466837908 1116 20-21 0.1376333848011413 1116 22-23 0.10548026943740041 1116 24-25 0.07470185406637597 1116 26-27 0.1357481973762802 1116 28-29 0.25755618841515826 1116 30-31 0.15097598803532009 1116 32-33 0.16562217743323515 1116 34-35 0.11747352634618125 1116 36-37 0.2810236454222377 1116 38-39 0.18777359396499804 1116 40-41 1.3171403371247195 1116 42-43 0.5096852162461047 1116 44-45 0.23519188071026775 1116 46-47 0.6714140930214967 1116 48-49 0.40117661360220325 1116 50-51 0.2293773261875529 1116 52-53 0.26457850305200026 1116 54-55 0.2779985637534068 1116 56-57 0.21722054403058166 1116 58-59 0.25968492279168487 1116 60-61 0.18811848600049785 1116 62-63 0.21711994089914555 1116 64-65 -0.18500780413188522 1116 66-67 0.11744016418379033 1116 68-69 0.12775199256598313 1116 70-71 0.2283896298933641 1116 72-73 0.2184929660677355 1116 74-75 0.21541705275615897 1116 76 0.47410387566686296 2118 1 0.04203833411081348 2118 2 -0.014359080438161698 2118 3 0.008564219569663578 2118 4 0.007678936745961096 2118 5 0.0870981093785943 2118 6 0.13120677414849524 2118 7 0.08460682121806684 2118 8 0.07014028794094429 2118 9 0.11176422436065536 2118 10-11 0.10726348303768418 2118 12-13 0.08337893298300258 2118 14-15 0.1379962315767571 2118 16-17 0.1725905988047316 2118 18-19 0.18376222180505408 2118 20-21 0.14007903772326102 2118 22-23 0.13818641052282743 2118 24-25 0.11471894652210324 2118 26-27 0.17360945926112947 2118 28-29 0.22762122665770335 2118 30-31 0.22692078675363803 2118 32-33 0.13286719391935264 2118 34-35 0.1711225645274368 2118 36-37 0.2502514789504815 2118 38-39 0.2240116830863883 2118 40-41 1.2973186012145348 2118 42-43 0.5042968967931571 2118 44-45 0.31132729059700637 2118 46-47 0.7678394916589113 2118 48-49 0.34935252248138937 2118 50-51 0.2126072245055184 2118 52-53 0.30902274864178736 2118 54-55 0.23918759795783018 2118 56-57 0.23809783904112436 2118 58-59 0.1393482224831999 2118 60-61 0.2935154785758556 2118 62-63 0.26848353052074714 2118 64-65 0.22693348726911466 2118 66-67 0.24026492533174348 2118 68-69 0.2556655840850155 2118 70-71 0.24109452717283375 2118 72-73 0.29632502185632603 2118 74-75 0.2664540984213133 2118 76 0.5468616161442981 1119 1 0.02011571988916927 1119 2 0.049090042537969225 1119 3 0.05205435081425236 1119 4 0.015278243468337394 1119 5 0.03895902628214998 1119 6 -0.025384767559110344 1119 7 0.03977408494272794 1119 8 -0.0027852715653082782 1119 9 0.10559473115289819 1119 10-11 0.04296541947164201 1119 12-13 0.103613806546214 1119 14-15 0.12027578976498177 1119 16-17 0.08256841850197105 1119 18-19 0.12102624590752953 1119 20-21 0.03478117601324726 1119 22-23 0.0632036076540814 1119 24-25 0.06617943213138489 1119 26-27 0.11557424134966965 1119 28-29 0.13089377571754568 1119 30-31 0.1660533510457327 1119 32-33 0.1499657235198839 1119 34-35 0.1505777489107487 1119 36-37 0.1787287412769416 1119 38-39 0.23966035673004882 1119 40-41 1.2564447841361783 1119 42-43 0.5144841242219087 1119 44-45 0.22015863487701637 1119 46-47 0.7050949857933944 1119 48-49 0.31249309921697943 1119 50-51 0.15616508250290906 1119 52-53 0.22373186718949256 1119 54-55 0.1790571097287028 1119 56-57 0.24435636006159456 1119 58-59 0.249347826406364 1119 60-61 0.24627660535241347 1119 62-63 0.22310123763671896 1119 64-65 0.23143051025063244 1119 66-67 0.23059146443621614 1119 68-69 0.1633264139471109 1119 70-71 0.312418570277444 1119 72-73 0.2749724540615759 1119 74-75 0.21499113136679426 1119 76 0.3758745902852496 2119 1 0.010907716696578973 2119 2 0.08240338213675358 2119 3 0.026127907747657275 2119 4 0.016563229102146693 2119 5 0.043508761457211165 2119 6 0.05696434657757976 2119 7 0.046656135675583243 2119 8 0.07654581315322417 2119 9 0.13442004562200793 2119 10-11 0.06638858374186896 2119 12-13 0.09256878894804288 2119 14-15 0.0996079069862148 2119 16-17 -0.01662327595023072 2119 18-19 0.07989202847974752 2119 20-21 0.04549963461636963 2119 22-23 0.09381758298638943 2119 24-25 0.10359438872545468 2119 26-27 0.05218679927187253 2119 28-29 0.14283767096126354 2119 30-31 0.15095915198209298 2119 32-33 0.1353754398025515 2119 34-35 0.12184571986044546 2119 36-37 0.16574480966031757 2119 38-39 0.09761637835786274 2119 40-41 1.3395147892602708 2119 42-43 0.49444894949603935 2119 44-45 0.2610830801709412 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64-65 -0.24037716470616743 2104 66-67 -0.2693022965605607 2104 68-69 -0.3684263569021553 2104 70-71 -0.279723943326907 2104 72-73 -0.16927019006498512 2104 74-75 -0.2553523555543009 2104 76 -0.34161928396335384 2111 1 -0.19433500656703018 2111 2 -0.1369486154962658 2111 3 -0.12450691933057811 2111 4 -0.07101178782804851 2111 5 -0.13488729780145547 2111 6 -0.03040727423491063 2111 7 -0.03641273508111453 2111 8 -0.1121088916743318 2111 9 -0.26880684807247945 2111 10-11 -0.23996207687989113 2111 12-13 -0.1068771124903023 2111 14-15 -0.1207246716427477 2111 16-17 -0.14701220153816053 2111 18-19 -0.11360284804218423 2111 20-21 -0.020151397911163826 2111 22-23 -0.18180402853949573 2111 24-25 -0.22583763995469752 2111 26-27 -0.09695230003400468 2111 28-29 -0.09203035924734593 2111 30-31 -0.22157859903871469 2111 32-33 -0.14247700241853067 2111 34-35 -0.1205201718315152 2111 36-37 -0.18418840471789366 2111 38-39 -0.25920856798114755 2111 40-41 0.9023417524159569 2111 42-43 -0.38427445931898774 2111 44-45 -0.36416441108212183 2111 46-47 0.04306114331089361 2111 48-49 -0.27828547935490633 2111 50-51 -0.551832721112774 2111 52-53 -0.5255709321566044 2111 54-55 -0.44219760612973147 2111 56-57 -0.651519599551662 2111 58-59 -0.521147758902913 2111 60-61 -0.7338307972143525 2111 62-63 -0.7758324094260303 2111 64-65 -0.5455213906030778 2111 66-67 -0.4456028717316727 2111 68-69 -0.4952660523711643 2111 70-71 -0.4201180082902738 2111 72-73 -0.7897891745615766 2111 74-75 -1.2071596236925117 2111 76 -0.6048592881077113 2110 1 -0.010917410859157428 2110 2 -0.029163494256351896 2110 3 -0.03494803229040144 2110 4 -0.05719655477778929 2110 5 -0.04994671390772254 2110 6 -0.05652390055439582 2110 7 -0.020942511354810733 2110 8 -0.11665312271308181 2110 9 -0.20146666756489395 2110 10-11 -0.176454878184245 2110 12-13 -0.17377817670239892 2110 14-15 -0.09101030310960567 2110 16-17 -0.22831790012376985 2110 18-19 -0.15369915748148344 2110 20-21 -0.3355112556242119 2110 22-23 -0.21051988650715003 2110 24-25 -0.19620858279414932 2110 26-27 -0.06730047890201973 2110 28-29 -0.29215927896773763 2110 30-31 -0.11506008943231905 2110 32-33 -0.11664285629011317 2110 34-35 -0.08267481954942468 2110 36-37 -0.19599134550901454 2110 38-39 -0.08371964925409259 2110 40-41 0.784457741344248 2110 42-43 -0.17585613573491088 2110 44-45 -0.3999304993583692 2110 46-47 -0.17703338497251764 2110 48-49 -0.4056812267881469 2110 50-51 -0.6208243956362267 2110 52-53 -0.6216362831656213 2110 54-55 -0.5299229230627276 2110 56-57 -0.6900069101004718 2110 58-59 -0.70860984868402 2110 60-61 -0.4308169687082639 2110 62-63 -0.571409020109023 2110 64-65 -0.5412649204258813 2110 66-67 -0.9995344664970176 2110 68-69 -0.9082554456449614 2110 70-71 -0.6812082012859406 2110 72-73 -1.0782109048917192 2110 74-75 -0.5685895493378155 2110 76 -0.8199459924355494 2109 1 -0.02987239392312091 2109 2 -0.0414433603819333 2109 3 -0.12810753359735116 2109 4 -0.07060902628800392 2109 5 -0.07247330032296162 2109 6 0.004076651515077856 2109 7 -0.11280159728346462 2109 8 -0.08497887433711071 2109 9 -0.0896313933214472 2109 10-11 -0.16801659822921522 2109 12-13 -0.2714198709856177 2109 14-15 -0.19416749945738587 2109 16-17 -0.390334489105463 2109 18-19 -0.32787793639106866 2109 20-21 -0.3922896847926651 2109 22-23 -0.39394924735355374 2109 24-25 -0.3550094735469287 2109 26-27 -0.34021672296171346 2109 28-29 -0.43300199744480494 2109 30-31 -0.36197643136871704 2109 32-33 -0.5788011049993145 2109 34-35 -0.282796343571988 2109 36-37 -0.6803162035410892 2109 38-39 -0.3184449986123141 2109 40-41 -10.492495842306901 2109 42-43 -1.1060519974926493 2109 44-45 -0.48891462502609073 2109 46-47 -9.642033226811009E-4 2109 48-49 -0.2966731325336269 2109 50-51 -0.4195362708830217 2109 52-53 -0.2917371522136847 2109 54-55 -0.4865079896917379 2109 56-57 -0.32025473617280653 2109 58-59 -0.4367704725845414 2109 60-61 -0.5932265588327255 2109 62-63 -0.34618731254894186 2109 64-65 -0.5276095503006175 2109 66-67 -0.5345411553615236 2109 68-69 -0.8915553253163822 2109 70-71 -0.6956772556213338 2109 72-73 -0.4305992647358252 2109 74-75 -0.6537861078133602 2109 76 -0.5227924489063795 2108 1 -0.009048588103681254 2108 2 -0.03960386264889593 2108 3 -0.040651242295091095 2108 4 -0.002855240820117899 2108 5 -0.049787602201746495 2108 6 -0.0019176280477211094 2108 7 -0.17867934651143713 2108 8 -0.25764885697149964 2108 9 -0.22262055749327914 2108 10-11 -0.2982991626823548 2108 12-13 -0.33291594604278174 2108 14-15 -0.2982370247711046 2108 16-17 -0.6048015769471746 2108 18-19 -0.5729448450762362 2108 20-21 -0.6032917042327242 2108 22-23 -0.3316465749350215 2108 24-25 -0.673031993839551 2108 26-27 -0.6480119973148248 2108 28-29 -1.2595438831460726 2108 30-31 -0.5884635093096762 2108 32-33 -1.06534845183225 2108 34-35 -0.562926074915751 2108 36-37 -1.3185075867396634 2108 38-39 -0.6092510459316429 2108 40-41 -7.973217065286086 2108 42-43 -0.7818466117953164 2108 44-45 -0.4391106757242582 2108 46-47 0.29373103409903933 2108 48-49 -0.13119259223518753 2108 50-51 -0.2671058708452989 2108 52-53 -0.5102470490415172 2108 54-55 -0.5554999590779701 2108 56-57 -0.44574289848235793 2108 58-59 -0.39782121003375437 2108 60-61 -0.6519478682470847 2108 62-63 -0.5212038138101107 2108 64-65 -0.25118356631469396 2108 66-67 -0.3631608248648348 2108 68-69 -0.3151250195715889 2108 70-71 -0.27645112548138684 2108 72-73 -0.46171199978056876 2108 74-75 -0.5680092127220959 2108 76 -1.301993002091514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 1.0 16 4.0 17 6.0 18 39.0 19 85.0 20 183.0 21 289.0 22 407.0 23 563.0 24 863.0 25 1297.0 26 1868.0 27 2858.0 28 4351.0 29 6463.0 30 9702.0 31 14385.0 32 20891.0 33 31269.0 34 46538.0 35 76132.0 36 165589.0 37 1109933.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26369519795249 25.32300295169701 7.83481743864467 24.57848441170583 2 15.304907154434208 22.812821973640254 41.30748997300024 20.574780898925297 3 17.824996301173517 27.800179016507144 32.42736073834602 21.94746394397332 4 13.238116724921788 17.434962579926452 41.6274300955268 27.699490599624966 5 15.488676904661325 30.24689415732699 33.45205283196215 20.81237610604954 6 24.979363560835154 37.38720252899311 20.820744491761157 16.81268941841058 7 22.172807834415757 34.69144422939553 25.126647149359616 18.009100786829098 8 19.662225173844845 39.82548233701564 23.611366811785633 16.900925677353875 9 20.68959515088869 18.715325593803914 25.327957036038285 35.26712221926911 10-11 22.70979040875882 26.356632481253143 30.991178382518243 19.942398727469797 12-13 20.87162427688779 25.123433689246355 31.229409586956564 22.77553244690929 14-15 20.515767042886974 27.944282618461198 25.808837952570666 25.73111238608117 16-17 20.26404600068152 32.16850983151427 27.294293363468448 20.27315080433576 18-19 19.733691187822057 31.17551718297375 29.57668688245498 19.51410474674922 20-21 19.942298306841256 29.290655458831893 29.458592223292634 21.308454011034218 22-23 19.96726956980472 29.470207542660358 29.942920914738202 20.619601972796723 24-25 19.889677897486603 30.55930273271309 29.413536834621283 20.137482535179018 26-27 19.43149204300413 30.531620112779063 29.61849533747022 20.418392506746592 28-29 19.348444183202037 30.57547045390794 29.746598585943655 20.32948677694637 30-31 19.473233550933678 30.501159188788773 30.08484873640723 19.94075852387032 32-33 19.564951058333005 30.22573887824802 30.15269960775703 20.05661045566195 34-35 19.760871704613393 29.691768922761142 30.453459390232556 20.093899982392916 36-37 20.229200042578345 30.08066454355142 30.160498943240256 19.52963647062998 38-39 19.829458993905806 29.81103515592311 29.855789282708837 20.503716567462245 40-41 20.132193715409276 29.755201286455197 29.634428743865136 20.478176254270387 42-43 20.42953919651447 29.718915966009625 29.55918021954627 20.292364617929636 44-45 19.824906592078687 29.437001788156657 29.886283680241977 20.851807939522683 46-47 19.842178940187466 29.57126416851385 30.284585366572113 20.30197152472657 48-49 19.968675458604274 29.659065271400138 29.765377243480522 20.606882026515063 50-51 19.8223960763652 29.67402794505251 30.033667689394978 20.46990828918731 52-53 19.056086259980972 30.026805613111453 30.508824630100616 20.40828349680696 54-55 19.146029669609437 29.729560552634805 30.497242784275734 20.627166993480024 56-57 19.01059571525262 29.421470064275894 30.962625450470206 20.60530877000128 58-59 18.65212754490978 29.820240380205888 31.132570627501728 20.395061447382602 60-61 19.608131928604948 29.113346102374145 30.869301213014243 20.40922075600666 62-63 19.375490805821986 28.425665638136273 30.97109425681036 21.22774929923138 64-65 19.19764587267869 28.38158098220747 31.11466228207887 21.306110863034966 66-67 18.974979865663975 28.800000267788345 30.525460980895307 21.69955888565237 68-69 19.139970958354226 28.532546660445053 29.98191759215434 22.345564789046385 70-71 19.099200183167227 28.549718587925287 30.1045646531438 22.246516575763682 72-73 18.604662061153483 28.750760686261184 30.241973546528563 22.40260370605677 74-75 18.843261474563118 28.464997050980873 29.68132517739304 23.010416297062964 76 18.818758841199504 28.036033599403364 30.08709146377794 23.058116095619184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 1.0 3 1.0 4 0.0 5 1.0 6 2.0 7 3.0 8 4.0 9 4.5 10 15.5 11 47.0 12 68.0 13 118.0 14 234.0 15 487.0 16 889.0 17 1104.0 18 1643.5 19 2868.0 20 4571.0 21 5589.0 22 7057.5 23 10572.0 24 14846.5 25 17075.0 26 19876.5 27 25864.5 28 32327.5 29 35604.0 30 39777.0 31 48276.5 32 56019.0 33 59435.0 34 62204.5 35 68948.0 36 75573.5 37 78225.0 38 80009.5 39 83544.5 40 87176.5 41 89806.0 42 90554.0 43 91428.5 44 90663.0 45 84703.5 46 80384.0 47 74948.5 48 64700.0 49 56026.0 50 52165.0 51 48421.5 52 40082.0 53 31850.0 54 28214.0 55 25125.5 56 19468.5 57 14774.5 58 12649.0 59 11376.5 60 8708.5 61 6132.0 62 4951.0 63 4345.0 64 3081.0 65 1951.0 66 1232.0 67 985.0 68 797.0 69 494.0 70 299.0 71 219.0 72 179.5 73 100.0 74 39.5 75 19.0 76 13.0 77 6.0 78 4.5 79 4.0 80 2.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1493717.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.45697459371655 #Duplication Level Percentage of deduplicated Percentage of total 1 93.66010452495279 80.9756927735868 2 4.813182719259048 8.322664321477902 3 0.6367951829239544 1.6516615485437227 4 0.2376449407537386 0.8218425042028502 5 0.1415288193305252 0.6118076768568957 6 0.1037789150582319 0.5383446613531815 7 0.07052528414285394 0.42681818895473556 8 0.04913700989558189 0.33985897729228176 9 0.038929031061850874 0.30291176245251994 >10 0.22562847210807002 3.6195625973435104 >50 0.01503568597011777 0.890932880026855 >100 0.007081413198470431 1.032571892816696 >500 4.7292573705567665E-4 0.31449811225107555 >1k 1.5507560772004032E-4 0.1508321028409859 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.6778834277175665E-4 0.0 0.0 2 3.347354284646958E-4 0.0 0.0 3 3.347354284646958E-4 0.0 0.0 4 3.347354284646958E-4 0.0 0.0 5 4.0168251415763497E-4 6.694708569293916E-5 0.0 6 4.0168251415763497E-4 6.694708569293916E-5 0.0 7 4.0168251415763497E-4 6.694708569293916E-5 0.0 8 4.0168251415763497E-4 6.694708569293916E-5 6.694708569293916E-5 9 4.686295998505741E-4 6.694708569293916E-5 6.694708569293916E-5 10 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 11 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 12 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 13 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 14 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 15 6.694708569293916E-4 6.694708569293916E-5 6.694708569293916E-5 16 7.364179426223307E-4 6.694708569293916E-5 6.694708569293916E-5 17 7.364179426223307E-4 6.694708569293916E-5 6.694708569293916E-5 18 7.364179426223307E-4 6.694708569293916E-5 1.3389417138587832E-4 19 7.364179426223307E-4 6.694708569293916E-5 1.3389417138587832E-4 20 7.364179426223307E-4 6.694708569293916E-5 2.0084125707881749E-4 21 7.364179426223307E-4 6.694708569293916E-5 3.347354284646958E-4 22 7.364179426223307E-4 2.6778834277175665E-4 4.0168251415763497E-4 23 7.364179426223307E-4 2.6778834277175665E-4 4.0168251415763497E-4 24 7.364179426223307E-4 2.6778834277175665E-4 4.0168251415763497E-4 25 7.364179426223307E-4 2.6778834277175665E-4 4.686295998505741E-4 26 7.364179426223307E-4 3.347354284646958E-4 9.372591997011482E-4 27 8.033650283152699E-4 3.347354284646958E-4 0.002075359656481114 28 8.70312114008209E-4 3.347354284646958E-4 0.004552401827119862 29 8.70312114008209E-4 3.347354284646958E-4 0.007498073597609185 30 8.70312114008209E-4 3.347354284646958E-4 0.01024290411101969 31 0.0010042062853940874 4.686295998505741E-4 0.012853840453044319 32 0.0010042062853940874 4.686295998505741E-4 0.017071506851699485 33 0.0010042062853940874 4.686295998505741E-4 0.02242727370713462 34 0.0010042062853940874 4.686295998505741E-4 0.030260082733208498 35 0.0010042062853940874 4.686295998505741E-4 0.04157414021531522 36 0.0010711533710870266 4.686295998505741E-4 0.06594287940754508 37 0.0012050475424729048 4.686295998505741E-4 0.1092576438508767 38 0.0012050475424729048 5.355766855435133E-4 0.17071506851699486 39 0.0012050475424729048 5.355766855435133E-4 0.2517210422054512 40 0.0012050475424729048 5.355766855435133E-4 0.3453800150898731 41 0.0012050475424729048 6.025237712364524E-4 0.45162504008456755 42 0.0012050475424729048 6.025237712364524E-4 0.5825735396999565 43 0.001271994628165844 6.025237712364524E-4 0.7336731121089203 44 0.001271994628165844 6.025237712364524E-4 0.9023797680551269 45 0.0013389417138587832 6.025237712364524E-4 1.1085098449036865 46 0.0013389417138587832 6.025237712364524E-4 1.3549420673393957 47 0.0013389417138587832 6.025237712364524E-4 1.6157009661133936 48 0.0013389417138587832 6.025237712364524E-4 1.8988201915088334 49 0.0013389417138587832 6.694708569293916E-4 2.2079818332388266 50 0.0013389417138587832 7.364179426223307E-4 2.5513534357579113 51 0.0013389417138587832 7.364179426223307E-4 2.8755781717688156 52 0.0013389417138587832 7.364179426223307E-4 3.230866355541244 53 0.0013389417138587832 7.364179426223307E-4 3.5897696819410907 54 0.0014728358852446615 7.364179426223307E-4 3.9759874193036566 55 0.0014728358852446615 7.364179426223307E-4 4.38811367882939 56 0.0015397829709376007 7.364179426223307E-4 4.818516492749296 57 0.0015397829709376007 7.364179426223307E-4 5.261036729179624 58 0.0015397829709376007 7.364179426223307E-4 5.729867170287276 59 0.0016067300566305399 7.364179426223307E-4 6.215434382818164 60 0.0016067300566305399 7.364179426223307E-4 6.791112372691749 61 0.001740624228016418 7.364179426223307E-4 7.317383413323943 62 0.001740624228016418 7.364179426223307E-4 7.856976924009032 63 0.0018075713137093573 7.364179426223307E-4 8.399984736064463 64 0.0019414654850952355 7.364179426223307E-4 8.977938926851605 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGCG 80 0.002667517 21.875 7 ATGCCGT 340 0.0 20.588236 14 TGGGGTC 600 0.0 19.833334 66 TACCGGC 645 0.0 19.534885 52 ATACCGG 665 0.0 18.947369 51 CCGGCAG 675 0.0 18.666668 54 ACCGGCA 710 0.0 18.239437 53 CGTATGC 365 0.0 18.219177 11 GGCAGTA 755 0.0 17.615894 56 CGGCAGT 760 0.0 17.5 55 GGGGTCT 630 0.0 17.222221 67 CAAGGGC 225 9.404903E-8 17.11111 4 GTATGCC 415 0.0 16.86747 12 GAGTCCT 770 0.0 16.818182 38 CTCGTAT 470 0.0 16.382978 9 TGTGCGG 365 1.8189894E-12 16.301369 10 TGAGTCC 775 0.0 16.258066 37 AAGGGCC 195 7.838769E-6 16.153847 5 CCTATAC 375 3.6379788E-12 15.866666 3 TACACCG 355 2.1827873E-11 15.774649 5 >>END_MODULE