##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve10.3410000000c7a4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456613 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.77514686467854 34.0 34.0 34.0 34.0 34.0 2 33.777841471962695 34.0 34.0 34.0 34.0 34.0 3 33.80024824713222 34.0 34.0 34.0 34.0 34.0 4 33.80791672187465 34.0 34.0 34.0 34.0 34.0 5 33.821092493339 34.0 34.0 34.0 34.0 34.0 6 37.61926400492101 38.0 38.0 38.0 37.0 38.0 7 37.640855875925865 38.0 38.0 38.0 37.0 38.0 8 37.57151487732157 38.0 38.0 38.0 37.0 38.0 9 37.53480368498702 38.0 38.0 38.0 37.0 38.0 10-11 37.537711458019395 38.0 38.0 38.0 37.0 38.0 12-13 37.47374765981081 38.0 38.0 38.0 37.0 38.0 14-15 37.529310118748086 38.0 38.0 38.0 37.0 38.0 16-17 37.48557235175026 38.0 38.0 38.0 37.0 38.0 18-19 37.504924094457486 38.0 38.0 38.0 37.0 38.0 20-21 37.45680321403145 38.0 38.0 38.0 37.0 38.0 22-23 37.512198847600565 38.0 38.0 38.0 37.0 38.0 24-25 37.44824260115762 38.0 38.0 38.0 37.0 38.0 26-27 37.41645653306678 38.0 38.0 38.0 37.0 38.0 28-29 37.3537552527679 38.0 38.0 38.0 37.0 38.0 30-31 37.36734225219739 38.0 38.0 38.0 37.0 38.0 32-33 37.32453266584879 38.0 38.0 38.0 37.0 38.0 34-35 37.345028157787965 38.0 38.0 38.0 37.0 38.0 36-37 37.259133345645 38.0 38.0 38.0 37.0 38.0 38-39 37.335137747637845 38.0 38.0 38.0 37.0 38.0 40-41 36.06549955272952 38.0 37.5 38.0 33.0 38.0 42-43 36.85983648367824 38.0 37.5 38.0 35.0 38.0 44-45 37.10788692672659 38.0 38.0 38.0 36.0 38.0 46-47 36.65854623019292 38.0 38.0 38.0 35.0 38.0 48-49 37.01267975776682 38.0 38.0 38.0 35.5 38.0 50-51 37.09705632175465 38.0 38.0 38.0 36.0 38.0 52-53 37.11461108750231 38.0 38.0 38.0 36.0 38.0 54-55 37.1161849441135 38.0 38.0 38.0 36.0 38.0 56-57 37.12222326726454 38.0 38.0 38.0 36.0 38.0 58-59 37.09463323477135 38.0 38.0 38.0 36.0 38.0 60-61 37.07894924732925 38.0 38.0 38.0 36.0 38.0 62-63 37.08944654482694 38.0 38.0 38.0 36.0 38.0 64-65 37.02067639105239 38.0 38.0 38.0 36.0 38.0 66-67 37.00077474250195 38.0 38.0 38.0 36.0 38.0 68-69 36.98679814061799 38.0 38.0 38.0 36.0 38.0 70-71 36.904780130343475 38.0 38.0 38.0 35.5 38.0 72-73 36.94006747159335 38.0 38.0 38.0 36.0 38.0 74-75 36.94208036039771 38.0 38.0 38.0 36.0 38.0 76 36.21307718659658 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03350472441555752 1101 2 0.057489362745620554 1101 3 0.01921413367033864 1101 4 -0.012374285858598455 1101 5 0.028542100079022248 1101 6 0.05526076857566409 1101 7 -0.006629243570969834 1101 8 0.03470171832275781 1101 9 0.04929695226745423 1101 10-11 0.03309895596071044 1101 12-13 0.03410546566993844 1101 14-15 0.05773112416330406 1101 16-17 0.06299899936224307 1101 18-19 0.03223970848567603 1101 20-21 0.03320187964912691 1101 22-23 -0.03199132721577769 1101 24-25 0.0037459694180839165 1101 26-27 0.017382165638771596 1101 28-29 0.04041276438277208 1101 30-31 0.01694775148121863 1101 32-33 0.06407459963953954 1101 34-35 -0.009613904786306193 1101 36-37 -0.010501655775193797 1101 38-39 0.007790512561172136 1101 40-41 1.2591152316609282 1101 42-43 0.4650016669823529 1101 44-45 0.14162913861122917 1101 46-47 0.7104568101443505 1101 48-49 0.24473304561964682 1101 50-51 0.0855638211184413 1101 52-53 0.154724099120628 1101 54-55 0.11249496652024504 1101 56-57 0.07837672603102419 1101 58-59 0.06007588198233549 1101 60-61 0.07182463686478968 1101 62-63 0.05499156656689763 1101 64-65 0.003199435822779151 1101 66-67 0.06981434451018487 1101 68-69 0.09910457851886889 1101 70-71 0.17907994262831295 1101 72-73 0.1613352973814699 1101 74-75 0.17816110659384776 1101 76 0.03549494507514339 1102 1 -0.02980242758658136 1102 2 0.03946739873308758 1102 3 0.05423583230389539 1102 4 0.05146991066754225 1102 5 0.070180190807406 1102 6 0.033244460777687834 1102 7 0.06648149230930755 1102 8 0.019347203237785493 1102 9 0.035991449036075096 1102 10-11 0.07793512347207354 1102 12-13 0.106640368208204 1102 14-15 0.07553480096293441 1102 16-17 0.12939785875302334 1102 18-19 0.1247812585070136 1102 20-21 0.12729264446091548 1102 22-23 0.0798223299145917 1102 24-25 0.09415863371888378 1102 26-27 0.14528565875453125 1102 28-29 0.09107705738197325 1102 30-31 0.13442113553141866 1102 32-33 0.1795100943371608 1102 34-35 0.11132995975594895 1102 36-37 -0.039187821725512606 1102 38-39 0.04891924477818321 1102 40-41 1.3432408860022136 1102 42-43 0.622033689378263 1102 44-45 0.2536936699311454 1102 46-47 0.7946750219063574 1102 48-49 0.40919255711419567 1102 50-51 0.2206062037012586 1102 52-53 0.248800359150394 1102 54-55 0.24253892316460224 1102 56-57 0.23470321761694635 1102 58-59 0.2759568621153434 1102 60-61 0.2804425587913357 1102 62-63 0.19299097336684667 1102 64-65 0.21634258956565589 1102 66-67 0.30754238830292024 1102 68-69 0.1362902215164965 1102 70-71 0.33508368655321163 1102 72-73 0.26914036611982795 1102 74-75 0.3180856166834545 1102 76 0.4099217785316256 1103 1 -0.049023913223159354 1103 2 -0.043707851989331914 1103 3 0.013368927663407248 1103 4 0.013264913217263086 1103 5 -0.04180349098759706 1103 6 0.020778032084841414 1103 7 0.064907465452265 1103 8 0.02072405178444825 1103 9 0.09519253691640728 1103 10-11 0.09065992374404885 1103 12-13 0.10643299101970172 1103 14-15 0.013508623842419354 1103 16-17 0.10795471749855778 1103 18-19 0.027728734284679035 1103 20-21 0.10016022902661348 1103 22-23 0.03289219222973827 1103 24-25 0.09750896518242769 1103 26-27 0.08638373796581789 1103 28-29 0.08485234210065329 1103 30-31 0.08646771040818635 1103 32-33 0.12584699727784 1103 34-35 0.09375729274931643 1103 36-37 -0.013884405101343589 1103 38-39 0.022492251747415537 1103 40-41 1.3526051559325225 1103 42-43 0.4906466419534823 1103 44-45 0.18482914502889258 1103 46-47 0.6974644150731137 1103 48-49 0.3075080424108094 1103 50-51 0.11864461947551774 1103 52-53 0.10072284767665707 1103 54-55 0.0910209901372383 1103 56-57 0.05984685181718419 1103 58-59 0.12645150770432423 1103 60-61 0.10395947193089228 1103 62-63 0.20145094277020803 1103 64-65 0.16208115832309034 1103 66-67 0.2007346371555485 1103 68-69 0.1284523836786633 1103 70-71 0.21575511269633552 1103 72-73 0.2030499360671314 1103 74-75 0.1250939457672544 1103 76 0.16035183122583163 2116 1 0.011997202590983136 2116 2 -0.05709283895635764 2116 3 -0.0037418167706775307 2116 4 0.016766838271664142 2116 5 0.03787223897868586 2116 6 0.0023204669797394217 2116 7 0.06390404579040165 2116 8 0.09648749727312378 2116 9 0.07849789119816108 2116 10-11 0.08914283739160567 2116 12-13 0.06611398200422514 2116 14-15 -0.005938012692965344 2116 16-17 0.09594046492185981 2116 18-19 0.06452094797766961 2116 20-21 0.1050142357702839 2116 22-23 0.07977408237011474 2116 24-25 0.06882350233238554 2116 26-27 0.06762772964384567 2116 28-29 0.01913696318776914 2116 30-31 0.07864170662980996 2116 32-33 0.09030403628111117 2116 34-35 0.01669102783313292 2116 36-37 0.037701313625483124 2116 38-39 0.06389598707036726 2116 40-41 1.304618451960451 2116 42-43 0.44110937242194836 2116 44-45 0.2658277819468893 2116 46-47 0.6950696952223367 2116 48-49 0.352433312037995 2116 50-51 0.12015261861462534 2116 52-53 0.14036127224083117 2116 54-55 0.09985997906518662 2116 56-57 0.06460137748856454 2116 58-59 0.17517475423085926 2116 60-61 0.21130337512815345 2116 62-63 0.19419838998067007 2116 64-65 0.22052341864784353 2116 66-67 0.22136500279631122 2116 68-69 0.14980829156301212 2116 70-71 0.22422152587758148 2116 72-73 0.18494581573329327 2116 74-75 0.14566404883538553 2116 76 0.2765026789671765 1117 1 -0.018707136616285425 1117 2 -0.008097545312345744 1117 3 0.018058886774191762 1117 4 -0.007209705553783863 1117 5 0.043410381899676054 1117 6 -0.00205635157720252 1117 7 0.05896806633210616 1117 8 0.0680918979536358 1117 9 0.1247107787595425 1117 10-11 0.050581311349375824 1117 12-13 0.055882466805144304 1117 14-15 0.044992523815032825 1117 16-17 0.125617336402577 1117 18-19 0.1416333451248306 1117 20-21 0.09653826504647611 1117 22-23 0.08577329058704919 1117 24-25 0.08356208791179398 1117 26-27 0.11394490160655835 1117 28-29 0.1716591686832274 1117 30-31 0.17088966552344687 1117 32-33 0.1343759513657261 1117 34-35 0.16973637798978558 1117 36-37 0.20119245806464647 1117 38-39 0.09201717390278219 1117 40-41 1.3762009685481758 1117 42-43 0.5514751589716482 1117 44-45 0.30868248209812776 1117 46-47 0.6749801937489863 1117 48-49 0.37650259209920023 1117 50-51 0.17966398446593246 1117 52-53 0.17382249672281347 1117 54-55 0.21559301482589888 1117 56-57 0.11216259313396648 1117 58-59 0.1814345298965776 1117 60-61 0.3012574281036109 1117 62-63 0.16056477244124068 1117 64-65 0.24762616573950424 1117 66-67 0.21543195727389275 1117 68-69 0.21949607956931771 1117 70-71 0.26325741993687757 1117 72-73 0.20576122468669666 1117 74-75 0.2538656389731244 1117 76 0.5275171151311824 2117 1 -0.0038738911905511486 2117 2 0.04546252016145047 2117 3 0.048994453441558505 2117 4 0.06482121654618567 2117 5 0.05319470634445622 2117 6 0.01210920221663514 2117 7 0.08148560109301428 2117 8 -0.018814465220721388 2117 9 0.11554906457734404 2117 10-11 -0.02763515296948782 2117 12-13 -0.0860334593268135 2117 14-15 -0.06492746156398965 2117 16-17 0.09313678696135952 2117 18-19 0.126091911787384 2117 20-21 0.06582861824265507 2117 22-23 0.07911259843091756 2117 24-25 0.048012510429394695 2117 26-27 0.10447611468335793 2117 28-29 0.12181745046102321 2117 30-31 0.06537489303848076 2117 32-33 0.1455000838459597 2117 34-35 0.10589044982178564 2117 36-37 0.17936640609923415 2117 38-39 0.025056046163150825 2117 40-41 1.3085679809134945 2117 42-43 0.5415491777063934 2117 44-45 0.29877378309322467 2117 46-47 0.8049178461511133 2117 48-49 0.3442936851620857 2117 50-51 0.19386714881895273 2117 52-53 0.14049931685103445 2117 54-55 0.20671942381908082 2117 56-57 0.1986394648845291 2117 58-59 0.29618110751204085 2117 60-61 0.2927797259067759 2117 62-63 0.151764606993261 2117 64-65 -0.2493181125978694 2117 66-67 0.0638712793860492 2117 68-69 0.1437173758671051 2117 70-71 0.30713515515918033 2117 72-73 0.17739356463741274 2117 74-75 0.15636743052468915 2117 76 0.4611650347670704 1116 1 0.04430623806707956 1116 2 0.0633696164408235 1116 3 0.0695200237977005 1116 4 0.04594837788942385 1116 5 0.060423143667719614 1116 6 0.11313040517303108 1116 7 0.13129513058041198 1116 8 0.0827318891565838 1116 9 0.14200147272982377 1116 10-11 0.04979081231367388 1116 12-13 0.0192309774228292 1116 14-15 0.11493561106810546 1116 16-17 0.13449391151886658 1116 18-19 0.17204272556905664 1116 20-21 0.16699789868917492 1116 22-23 0.11461813240414642 1116 24-25 0.06929738477299452 1116 26-27 0.1492487859271563 1116 28-29 0.24200355437951515 1116 30-31 0.193346714810545 1116 32-33 0.18485050866239305 1116 34-35 0.15649798646653323 1116 36-37 0.24604581269360182 1116 38-39 0.2597671500413625 1116 40-41 1.3908565596112794 1116 42-43 0.5222400341227882 1116 44-45 0.2778628890048864 1116 46-47 0.7500503183039982 1116 48-49 0.4192633687531995 1116 50-51 0.21116404982616643 1116 52-53 0.2143514746091526 1116 54-55 0.2465158535241727 1116 56-57 0.22019464912740006 1116 58-59 0.24811177050926858 1116 60-61 0.1603755049732314 1116 62-63 0.22741899187549564 1116 64-65 -0.07025543183134175 1116 66-67 0.15047331186641344 1116 68-69 0.25731214626642895 1116 70-71 0.2716603323607316 1116 72-73 0.2576898162950272 1116 74-75 0.2136872440662927 1116 76 0.5181116092465032 2118 1 0.029491678334018445 2118 2 -0.04286902668345505 2118 3 0.01651085340535019 2118 4 0.017807891724480385 2118 5 0.04296716329802308 2118 6 0.09182717524304707 2118 7 0.06983147160515557 2118 8 0.0487808578319644 2118 9 0.10941154784390505 2118 10-11 0.08409843952377116 2118 12-13 0.019689083933997154 2118 14-15 0.09327934868871779 2118 16-17 0.09234152171149645 2118 18-19 0.0798917012199496 2118 20-21 0.053793021865175206 2118 22-23 0.12595156198178614 2118 24-25 0.09212302180971932 2118 26-27 0.11717180662606097 2118 28-29 0.16203922910478497 2118 30-31 0.1731414331423622 2118 32-33 0.0764978019047362 2118 34-35 0.13437850893311065 2118 36-37 0.1759668059550421 2118 38-39 0.1488933280329121 2118 40-41 1.285307488668444 2118 42-43 0.5803772412958565 2118 44-45 0.240115221526068 2118 46-47 0.685356861788037 2118 48-49 0.22895500665536872 2118 50-51 0.08006599371523038 2118 52-53 0.10193244581008543 2118 54-55 0.10120703511731222 2118 56-57 0.08445220596122027 2118 58-59 -0.007818212828361482 2118 60-61 0.08686497237071933 2118 62-63 0.07004570340487248 2118 64-65 0.10207974710959178 2118 66-67 0.08590936890738021 2118 68-69 0.11464064896798476 2118 70-71 0.16188326029045896 2118 72-73 0.09524761885455746 2118 74-75 0.05142681340013411 2118 76 0.33101797103945074 1119 1 0.019820120460877888 1119 2 0.016317507360227523 1119 3 0.01197981639333534 1119 4 0.01587165223544673 1119 5 0.040742612973105 1119 6 -0.025196352761447827 1119 7 -0.0037872544027166555 1119 8 0.004462487305218588 1119 9 0.07156614992894106 1119 10-11 0.08009735989169542 1119 12-13 0.11857181372057823 1119 14-15 0.11535594034636887 1119 16-17 0.07815448159028904 1119 18-19 0.13752735957423567 1119 20-21 0.056991826207713814 1119 22-23 0.08715118919324993 1119 24-25 0.0025161892810743325 1119 26-27 0.09454291130786885 1119 28-29 0.13329972145030666 1119 30-31 0.18472688422394157 1119 32-33 0.1259619512409884 1119 34-35 0.12816361395405806 1119 36-37 0.13449583355444616 1119 38-39 0.20052638107377874 1119 40-41 1.3377960537261373 1119 42-43 0.5118149089751114 1119 44-45 0.20559810493238473 1119 46-47 0.6713080618928515 1119 48-49 0.2924537156099589 1119 50-51 0.08281239541635443 1119 52-53 0.10607067026226247 1119 54-55 0.18411699406588156 1119 56-57 0.15527698965140502 1119 58-59 0.1576364967379078 1119 60-61 0.12179549515538213 1119 62-63 0.15494404316386579 1119 64-65 0.17463921560111828 1119 66-67 0.10970530968360492 1119 68-69 0.10752182503082253 1119 70-71 0.17836955741974947 1119 72-73 0.1032287463251933 1119 74-75 0.15523711713866106 1119 76 0.3888251699594747 2119 1 -0.04584623358979911 2119 2 0.05503896839852018 2119 3 -0.01957259261148181 2119 4 0.0059801880818639574 2119 5 0.05618151082708067 2119 6 -0.002680851739711443 2119 7 0.05425576869024695 2119 8 0.10198951777928755 2119 9 0.12595355420375398 2119 10-11 0.04563183946135041 2119 12-13 0.06739258578902962 2119 14-15 0.08915251985123263 2119 16-17 0.035526491498245605 2119 18-19 0.08428575048479559 2119 20-21 0.07616844299192849 2119 22-23 0.07764551403084141 2119 24-25 0.09644806274604178 2119 26-27 0.07526531577617135 2119 28-29 0.08888199281858533 2119 30-31 0.09802954217900606 2119 32-33 0.14198552231488293 2119 34-35 0.13145185201854304 2119 36-37 0.21057093264062843 2119 38-39 0.11814170962255588 2119 40-41 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52-53 -0.19412698625733782 2104 54-55 -0.29693473232558887 2104 56-57 -0.12433528573998132 2104 58-59 -0.20593630941107222 2104 60-61 -0.1774138787661812 2104 62-63 -0.09288544940802979 2104 64-65 -0.08206483842624834 2104 66-67 -0.2586003921710045 2104 68-69 -0.2951250515354076 2104 70-71 -0.14048886804202 2104 72-73 -0.05264133259636594 2104 74-75 -0.024711418820807296 2104 76 -0.24638994371183287 2111 1 -0.215229252266127 2111 2 -0.11442916225628608 2111 3 -0.06109313041803688 2111 4 -0.028076448449795066 2111 5 -0.06450028736077229 2111 6 0.00994393646189451 2111 7 0.020955890109568998 2111 8 -0.04433560784904955 2111 9 -0.1687938189371394 2111 10-11 -0.08007053399830255 2111 12-13 -0.02316589385949186 2111 14-15 -0.02406421251159685 2111 16-17 -0.06727673373333687 2111 18-19 -0.07622609125989754 2111 20-21 0.002836854043792414 2111 22-23 -0.08653382881950478 2111 24-25 -0.09552759456264681 2111 26-27 0.009946763401160297 2111 28-29 0.024284729524715942 2111 30-31 2.613326409033334E-4 2111 32-33 -0.10607852953967267 2111 34-35 -0.01850374202113869 2111 36-37 0.012032403133396485 2111 38-39 -0.06266012240778451 2111 40-41 1.2209991541164271 2111 42-43 -0.02247998524166661 2111 44-45 -0.16935078212637933 2111 46-47 0.4372235650615508 2111 48-49 -0.11271153050595473 2111 50-51 -0.305034642561111 2111 52-53 -0.265565890876438 2111 54-55 -0.18643658132532437 2111 56-57 -0.3051708232497461 2111 58-59 -0.24156821465471268 2111 60-61 -0.3402844875521538 2111 62-63 -0.38890691450907156 2111 64-65 -0.2917228511527554 2111 66-67 -0.1503932164044386 2111 68-69 -0.15992409022087628 2111 70-71 -0.14491439538392825 2111 72-73 -0.4921950185808299 2111 74-75 -0.718633265123934 2111 76 -0.13889753967581697 2110 1 0.030735469803389037 2110 2 0.0018666861656910783 2110 3 -0.013272020642688176 2110 4 -0.01119645413110959 2110 5 -0.017781206195166988 2110 6 0.027855791715033718 2110 7 0.04435228286860138 2110 8 0.0020334837426787544 2110 9 -0.07200359397334921 2110 10-11 -0.06959715966026181 2110 12-13 -0.07359643479284728 2110 14-15 0.03247791879193329 2110 16-17 -0.051992275958291145 2110 18-19 -0.04011633430317829 2110 20-21 -0.21197773494763794 2110 22-23 -0.0613470286041391 2110 24-25 -0.08456580510584644 2110 26-27 0.05134724795422585 2110 28-29 -0.07119437847437382 2110 30-31 -0.008877299728858645 2110 32-33 -0.07316034080503897 2110 34-35 0.01778348539653507 2110 36-37 -0.029474040268034685 2110 38-39 0.033379902592280075 2110 40-41 1.0817538781976097 2110 42-43 0.12160587000185075 2110 44-45 -0.19900178848079264 2110 46-47 0.17052196073990444 2110 48-49 -0.18139167936327993 2110 50-51 -0.2717089578816285 2110 52-53 -0.31273901065859633 2110 54-55 -0.25433844900209834 2110 56-57 -0.4061327004161299 2110 58-59 -0.3895419391237951 2110 60-61 -0.19685850553715056 2110 62-63 -0.31728481610512915 2110 64-65 -0.19806279769517232 2110 66-67 -0.6787100466589209 2110 68-69 -0.6412940065657224 2110 70-71 -0.36332437039801135 2110 72-73 -0.6221176841829106 2110 74-75 -0.2931247404587296 2110 76 -0.36749420597561056 2109 1 0.01578730509982762 2109 2 -0.03792517114611371 2109 3 -0.10535210976514975 2109 4 -0.017759404075263774 2109 5 0.00792813788176261 2109 6 0.05663405404620647 2109 7 -0.05259996084810581 2109 8 -0.05894427427623583 2109 9 -0.04496643712739257 2109 10-11 -0.14917546846704255 2109 12-13 -0.09981493473777903 2109 14-15 -0.0711967825042521 2109 16-17 -0.20603643240003322 2109 18-19 -0.22857983105652124 2109 20-21 -0.2577399205237114 2109 22-23 -0.2719243525431736 2109 24-25 -0.21086298491282918 2109 26-27 -0.14269519592474467 2109 28-29 -0.192277828140881 2109 30-31 -0.16307728785047715 2109 32-33 -0.3159702440308294 2109 34-35 -0.07688771984979326 2109 36-37 -0.38893736230537 2109 38-39 -0.17318471955122305 2109 40-41 -11.079108526897873 2109 42-43 -0.8031285818598448 2109 44-45 -0.20066641967723342 2109 46-47 0.3281681210530749 2109 48-49 -0.012244175236126864 2109 50-51 -0.03524901541682368 2109 52-53 -0.05431955905925889 2109 54-55 -0.2977447075252684 2109 56-57 -0.09121475062478623 2109 58-59 -0.18622001422630774 2109 60-61 -0.3049505520381359 2109 62-63 -0.11237365313662195 2109 64-65 -0.22190886482647443 2109 66-67 -0.1945960124664765 2109 68-69 -0.5266736073483216 2109 70-71 -0.30555867666305403 2109 72-73 -0.19052534101069796 2109 74-75 -0.24488546545516243 2109 76 -0.20208399529579424 2108 1 -0.0016427407624277635 2108 2 -0.023825630730321734 2108 3 -5.777742359214244E-4 2108 4 -0.023426071643491753 2108 5 -0.023147589082000763 2108 6 -0.015618906294562862 2108 7 -0.13273967528863295 2108 8 -0.11245226643748651 2108 9 -0.08555713190704495 2108 10-11 -0.20439991433194393 2108 12-13 -0.21893072874139818 2108 14-15 -0.18646874648521106 2108 16-17 -0.4882481975513926 2108 18-19 -0.39884574257821725 2108 20-21 -0.43529545424152616 2108 22-23 -0.22050241529340298 2108 24-25 -0.5515031451737826 2108 26-27 -0.5107402395646616 2108 28-29 -0.9889319135659065 2108 30-31 -0.4849617363659604 2108 32-33 -0.910471363916102 2108 34-35 -0.5233066639270447 2108 36-37 -1.0418201089798984 2108 38-39 -0.41183508124125723 2108 40-41 -8.068082472773739 2108 42-43 -0.5767274630864989 2108 44-45 -0.20818364769898068 2108 46-47 0.39669058472254193 2108 48-49 0.08356052236180034 2108 50-51 -0.18503137931988078 2108 52-53 -0.32526444141044664 2108 54-55 -0.31684992324856154 2108 56-57 -0.24919558230000405 2108 58-59 -0.2606212745442562 2108 60-61 -0.3376333436703476 2108 62-63 -0.31744653959169966 2108 64-65 -0.11621197252689797 2108 66-67 -0.2030755033747198 2108 68-69 -0.12322066281486599 2108 70-71 -0.14570996710806838 2108 72-73 -0.24125593354766295 2108 74-75 -0.23834402368964902 2108 76 -0.7642734649182472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 15.0 16 23.0 17 70.0 18 119.0 19 168.0 20 176.0 21 207.0 22 230.0 23 341.0 24 567.0 25 906.0 26 1541.0 27 2541.0 28 3984.0 29 5954.0 30 8606.0 31 12954.0 32 18974.0 33 28414.0 34 42269.0 35 65921.0 36 143404.0 37 1119224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39781808895019 25.841592790947214 8.49120528239141 24.269383837711185 2 17.0160502480755 23.022244068946247 38.13023775017798 21.83146793280027 3 19.797022270156862 24.92933950198165 31.285591986340915 23.988046241520568 4 14.819722191137934 16.278586007402104 38.99361051974684 29.908081281713123 5 13.676934092995188 35.80408797669662 36.36683182149273 14.15214610881545 6 28.856875505024327 37.541062725651905 18.13618304930685 15.465878720016915 7 24.669421459234538 33.602748293472594 22.58534010063071 19.14249014666215 8 21.954149798196227 39.1655848190288 22.01353413707004 16.866731245704933 9 21.28760350209699 18.328821725468604 24.779402627877136 35.604172144557275 10-11 24.832333639751944 26.22831184398327 28.605882276212007 20.33347224005278 12-13 22.28804081797979 24.46274336422921 29.604809238967384 23.644406578823613 14-15 21.247991058709488 27.94983979958987 24.611856409355127 26.190312732345518 16-17 20.63794570005897 32.18126571711223 26.83114869907107 20.34963988375773 18-19 20.164381342195902 30.61660852951333 29.36102451371778 19.857985614572986 20-21 20.44142816245633 28.755098299960252 29.69278730863997 21.110686228943447 22-23 20.805285961336335 29.180365683953113 29.260002485217417 20.754345869493132 24-25 20.78108598509007 29.574567850211412 29.06952636012448 20.574819804574034 26-27 20.49628144194786 29.62993602281457 29.19128141791952 20.682501117318054 28-29 20.340474786370848 29.39696405290904 29.639856296765167 20.62270486395494 30-31 20.662523264587094 29.11521454222913 29.396277528760212 20.825984664423565 32-33 20.589202485492027 29.484598860507216 29.15324798007432 20.77295067392643 34-35 20.451039500539952 29.317704839926595 29.423154949186912 20.808100710346537 36-37 20.248823812502017 29.47172653271665 29.678679237381516 20.600770417399815 38-39 20.23303375707892 29.020439883483125 29.81110974569086 20.935416613747098 40-41 20.21686611337397 28.655620950794752 29.9748800813943 21.15263285443697 42-43 20.297601353276402 28.393952271468127 29.949066773398286 21.359379601857185 44-45 19.714879655749332 28.1132668732189 30.664116000612378 21.507737470419393 46-47 19.564015974043894 28.034282269895982 31.196515478030197 21.205186278029924 48-49 19.25943953541538 28.056868914392496 31.263623213578352 21.420068336613774 50-51 18.831632012071843 28.24350050425199 31.032710816119312 21.892156667556858 52-53 18.706032419043357 28.056182390243666 31.179043438442466 22.058741752270507 54-55 18.116891720724723 27.916028485259986 31.395882090850485 22.57119770316481 56-57 17.931324243295922 27.863886976156333 31.415585333921914 22.789203446625837 58-59 17.81646875319663 27.948878665781507 31.12243952237142 23.112213058650443 60-61 17.882512376314093 27.617424806726287 30.777976030695868 23.72208678626375 62-63 17.557511844257878 27.247388290506812 30.793628781289193 24.401471083946113 64-65 17.737346845043948 27.159753482908638 30.1644980513012 24.938401620746212 66-67 17.992150282882275 27.57197690807373 29.34708807349653 25.088784735547463 68-69 17.972584344640616 27.77704167132931 28.897723691879722 25.352650292150354 70-71 17.95662265818031 27.747795742589144 28.58755208143824 25.7080295177923 72-73 18.177717760311076 27.59991844093112 28.247379365692876 25.974984433064925 74-75 18.672941955069742 27.8290458756032 27.437727110769984 26.060285058557074 76 19.135144338269672 28.12332445199926 26.547682878019074 26.193848331711994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 36.0 1 20.5 2 10.5 3 10.0 4 4.0 5 6.5 6 12.5 7 18.5 8 21.0 9 44.0 10 133.0 11 360.5 12 522.0 13 691.0 14 997.5 15 1515.5 16 2276.5 17 2657.0 18 3726.5 19 5755.5 20 7987.5 21 9260.0 22 11045.5 23 14917.5 24 19448.0 25 21892.0 26 24142.0 27 28775.0 28 33409.5 29 35661.0 30 38254.0 31 43264.5 32 47131.0 33 48580.0 34 50183.5 35 54086.5 36 58176.0 37 59966.0 38 61143.5 39 63652.5 40 66218.5 41 68020.5 42 68588.0 43 69180.0 44 69445.0 45 68292.0 46 67466.0 47 66028.5 48 62848.5 49 59653.0 50 58200.0 51 55662.0 52 50712.0 53 45160.0 54 42020.0 55 39600.0 56 34593.0 57 29244.0 58 26482.0 59 24000.0 60 19301.0 61 15014.0 62 12944.0 63 11462.0 64 8741.0 65 6538.5 66 4785.0 67 3995.0 68 3408.0 69 2406.5 70 1711.5 71 1431.0 72 1223.5 73 833.5 74 536.5 75 422.0 76 356.5 77 218.0 78 104.5 79 64.0 80 56.5 81 33.0 82 10.0 83 3.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1456613.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.639991952015464 #Duplication Level Percentage of deduplicated Percentage of total 1 64.07804762490451 40.779264351497865 2 24.193876563547644 30.793962195844554 3 7.962561240244985 15.202119997398633 4 2.3687891179036136 6.029988815976311 5 0.6874170403418308 2.18736074575162 6 0.2310928248524803 0.8824047308268218 7 0.11350085464696186 0.505623543339567 8 0.06224200833183687 0.3168864727452302 9 0.051040413507510464 0.2923390354360957 >10 0.24279366972943575 2.497213023293292 >50 0.0062231425115572584 0.25108632334104286 >100 0.002415499477662752 0.26175076454898005 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.3730482976603945E-4 0.0 4.119144892981183E-4 2 1.3730482976603945E-4 0.0 4.119144892981183E-4 3 1.3730482976603945E-4 0.0 4.119144892981183E-4 4 1.3730482976603945E-4 0.0 4.119144892981183E-4 5 1.3730482976603945E-4 0.0 4.119144892981183E-4 6 1.3730482976603945E-4 0.0 4.119144892981183E-4 7 1.3730482976603945E-4 0.0 4.119144892981183E-4 8 1.3730482976603945E-4 0.0 4.8056690418113804E-4 9 1.3730482976603945E-4 0.0 4.8056690418113804E-4 10 1.3730482976603945E-4 0.0 6.178717339471774E-4 11 1.3730482976603945E-4 0.0 6.178717339471774E-4 12 1.3730482976603945E-4 0.0 6.865241488301972E-4 13 1.3730482976603945E-4 6.865241488301972E-5 7.551765637132169E-4 14 1.3730482976603945E-4 6.865241488301972E-5 8.924813934792563E-4 15 1.3730482976603945E-4 6.865241488301972E-5 8.924813934792563E-4 16 1.3730482976603945E-4 6.865241488301972E-5 9.611338083622761E-4 17 1.3730482976603945E-4 6.865241488301972E-5 9.611338083622761E-4 18 1.3730482976603945E-4 6.865241488301972E-5 0.0011670910530113352 19 1.3730482976603945E-4 6.865241488301972E-5 0.0023341821060226704 20 1.3730482976603945E-4 6.865241488301972E-5 0.0024714869357887097 21 1.3730482976603945E-4 6.865241488301972E-5 0.0032266634995019266 22 1.3730482976603945E-4 6.865241488301972E-5 0.004599711797162321 23 1.3730482976603945E-4 6.865241488301972E-5 0.005148931116226479 24 2.0595724464905914E-4 6.865241488301972E-5 0.005972760094822715 25 2.0595724464905914E-4 6.865241488301972E-5 0.006316022169237814 26 2.0595724464905914E-4 6.865241488301972E-5 0.008375594615728406 27 2.0595724464905914E-4 6.865241488301972E-5 0.05320562153434028 28 2.0595724464905914E-4 6.865241488301972E-5 0.12206399366200905 29 2.0595724464905914E-4 6.865241488301972E-5 0.19861143625657604 30 2.0595724464905914E-4 6.865241488301972E-5 0.261153786215007 31 2.746096595320789E-4 6.865241488301972E-5 0.316899547100019 32 2.746096595320789E-4 6.865241488301972E-5 0.38459082817467644 33 2.746096595320789E-4 6.865241488301972E-5 0.46683642120453406 34 3.432620744150986E-4 1.3730482976603945E-4 0.5828590023568374 35 3.432620744150986E-4 2.746096595320789E-4 0.7384940268966431 36 3.432620744150986E-4 2.746096595320789E-4 0.9938810102614765 37 3.432620744150986E-4 2.746096595320789E-4 1.4116309548246515 38 4.119144892981183E-4 2.746096595320789E-4 1.87970311949708 39 4.119144892981183E-4 2.746096595320789E-4 2.390820348301162 40 4.119144892981183E-4 2.746096595320789E-4 2.952946321363327 41 4.119144892981183E-4 2.746096595320789E-4 3.5461718383675005 42 4.119144892981183E-4 2.746096595320789E-4 4.189582270651162 43 5.492193190641578E-4 2.746096595320789E-4 4.879401735395743 44 5.492193190641578E-4 2.746096595320789E-4 5.66526592856167 45 5.492193190641578E-4 2.746096595320789E-4 6.542918400426194 46 5.492193190641578E-4 2.746096595320789E-4 7.466979904751639 47 6.178717339471774E-4 2.746096595320789E-4 8.419051594349357 48 6.865241488301972E-4 2.746096595320789E-4 9.338101472388342 49 6.865241488301972E-4 2.746096595320789E-4 10.225914501655552 50 8.924813934792563E-4 2.746096595320789E-4 11.142561545173633 51 8.924813934792563E-4 2.746096595320789E-4 12.067446878477673 52 8.924813934792563E-4 2.746096595320789E-4 13.005582127854138 53 8.924813934792563E-4 2.746096595320789E-4 13.979691242629306 54 8.924813934792563E-4 2.746096595320789E-4 15.064330745366133 55 9.611338083622761E-4 2.746096595320789E-4 16.196065804712713 56 9.611338083622761E-4 2.746096595320789E-4 17.294847704915444 57 9.611338083622761E-4 2.746096595320789E-4 18.395002653415837 58 9.611338083622761E-4 2.746096595320789E-4 19.462616357261673 59 9.611338083622761E-4 2.746096595320789E-4 20.45732119650175 60 0.0010297862232452957 2.746096595320789E-4 21.473308284355557 61 0.0010984386381283156 2.746096595320789E-4 22.476869285115537 62 0.0010984386381283156 2.746096595320789E-4 23.512765573285424 63 0.0011670910530113352 2.746096595320789E-4 24.54941703801902 64 0.0012357434678943549 2.746096595320789E-4 25.66543069435739 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCACG 340 0.0 28.823528 40 GTCCTAA 165 6.8030204E-8 21.212122 1 CTGCTTG 970 0.0 20.927835 24 CTCGTAT 975 0.0 20.820515 9 TATGCCG 975 0.0 20.820515 13 AACCCAC 510 0.0 20.588234 39 TCGTATG 980 0.0 20.357143 10 GCCGTCT 1020 0.0 20.245096 16 GTATGCC 1015 0.0 20.000002 12 CGTATGC 1005 0.0 19.850746 11 TACACTA 300 0.0 19.833334 5 ATGCCGT 1025 0.0 19.804878 14 GGGTACA 160 1.1868578E-6 19.687498 1 CCGTCTT 1060 0.0 19.150942 17 TGCCGTC 1060 0.0 19.150942 15 TCTCGTA 1160 0.0 18.405172 8 GTCCTAT 195 3.9514998E-7 17.948717 1 TAGCGTA 120 0.0013413018 17.5 9 ACTAGAC 240 1.0300937E-8 17.5 3 TCTGCTT 1220 0.0 16.92623 23 >>END_MODULE