FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve10.3410000000c7a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve10.3410000000c7a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456613
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACG3400.028.82352840
GTCCTAA1656.8030204E-821.2121221
CTGCTTG9700.020.92783524
CTCGTAT9750.020.8205159
TATGCCG9750.020.82051513
AACCCAC5100.020.58823439
TCGTATG9800.020.35714310
GCCGTCT10200.020.24509616
GTATGCC10150.020.00000212
CGTATGC10050.019.85074611
TACACTA3000.019.8333345
ATGCCGT10250.019.80487814
GGGTACA1601.1868578E-619.6874981
CCGTCTT10600.019.15094217
TGCCGTC10600.019.15094215
TCTCGTA11600.018.4051728
GTCCTAT1953.9514998E-717.9487171
TAGCGTA1200.001341301817.59
ACTAGAC2401.0300937E-817.53
TCTGCTT12200.016.9262323
Produced by FastQC (version 0.11.2)