##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve09.3410000000c78a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1432747 Sequences flagged as poor quality 0 Sequence length 76 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.770024295985266 34.0 34.0 34.0 34.0 34.0 2 33.78636632985447 34.0 34.0 34.0 34.0 34.0 3 33.81027913511597 34.0 34.0 34.0 34.0 34.0 4 33.826427136123826 34.0 34.0 34.0 34.0 34.0 5 33.82918477581876 34.0 34.0 34.0 34.0 34.0 6 37.63230005018332 38.0 38.0 38.0 37.0 38.0 7 37.65440653513844 38.0 38.0 38.0 37.0 38.0 8 37.57584695692959 38.0 38.0 38.0 37.0 38.0 9 37.54808839243774 38.0 38.0 38.0 37.0 38.0 10-11 37.54741311620265 38.0 38.0 38.0 37.0 38.0 12-13 37.48410954620739 38.0 38.0 38.0 37.0 38.0 14-15 37.536231798077395 38.0 38.0 38.0 37.0 38.0 16-17 37.48966949503297 38.0 38.0 38.0 37.0 38.0 18-19 37.508145890377016 38.0 38.0 38.0 37.0 38.0 20-21 37.46279419883622 38.0 38.0 38.0 37.0 38.0 22-23 37.51513246930547 38.0 38.0 38.0 37.0 38.0 24-25 37.4516177664305 38.0 38.0 38.0 37.0 38.0 26-27 37.419767411831955 38.0 38.0 38.0 37.0 38.0 28-29 37.34796897149322 38.0 38.0 38.0 37.0 38.0 30-31 37.37752233995255 38.0 38.0 38.0 37.0 38.0 32-33 37.32990297658972 38.0 38.0 38.0 37.0 38.0 34-35 37.37095139616415 38.0 38.0 38.0 37.0 38.0 36-37 37.27774826958284 38.0 38.0 38.0 37.0 38.0 38-39 37.33992428530648 38.0 38.0 38.0 37.0 38.0 40-41 36.12448534179447 38.0 37.5 38.0 33.5 38.0 42-43 36.858101604819275 38.0 37.5 38.0 35.0 38.0 44-45 37.07458155557122 38.0 38.0 38.0 36.0 38.0 46-47 36.6439908790596 38.0 38.0 38.0 35.0 38.0 48-49 36.97958048420272 38.0 38.0 38.0 35.5 38.0 50-51 37.051914259810005 38.0 38.0 38.0 36.0 38.0 52-53 37.06625314867175 38.0 38.0 38.0 36.0 38.0 54-55 37.05986018466624 38.0 38.0 38.0 36.0 38.0 56-57 37.06486595330509 38.0 38.0 38.0 36.0 38.0 58-59 37.03560782189737 38.0 38.0 38.0 36.0 38.0 60-61 37.03482750269238 38.0 38.0 38.0 36.0 38.0 62-63 37.04178825710331 38.0 38.0 38.0 36.0 38.0 64-65 36.96780764503433 38.0 38.0 38.0 36.0 38.0 66-67 36.94533263723463 38.0 38.0 38.0 36.0 38.0 68-69 36.93648320324523 38.0 38.0 38.0 36.0 38.0 70-71 36.85663868079989 38.0 38.0 38.0 35.5 38.0 72-73 36.90416067875208 38.0 38.0 38.0 36.0 38.0 74-75 36.90200607643918 38.0 38.0 38.0 36.0 38.0 76 36.20013721892281 38.0 37.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.032978691363211965 1101 2 0.07079376336304222 1101 3 0.07004470252958583 1101 4 -0.004093068034499936 1101 5 0.026972546796876884 1101 6 0.07664137916226821 1101 7 0.004340284594064769 1101 8 0.05703258093944896 1101 9 0.036167596632282084 1101 10-11 0.015250790262925307 1101 12-13 0.08978842081273086 1101 14-15 0.05727553832790733 1101 16-17 0.026858149825102373 1101 18-19 0.015159016348796683 1101 20-21 0.10180894664018325 1101 22-23 0.019242327539437554 1101 24-25 0.0535582109225885 1101 26-27 0.03313618858188505 1101 28-29 0.10944851666322108 1101 30-31 0.12291790865327101 1101 32-33 0.1872032466509026 1101 34-35 0.08525973219536098 1101 36-37 0.019875931515066725 1101 38-39 0.03584999414747614 1101 40-41 1.290105184508569 1101 42-43 0.5061735775037164 1101 44-45 0.24662922663193143 1101 46-47 0.7252415742796856 1101 48-49 0.3392824329365425 1101 50-51 0.1978049897980796 1101 52-53 0.2443626014407343 1101 54-55 0.15027878008855566 1101 56-57 0.15557889120686497 1101 58-59 0.16893221846375184 1101 60-61 0.2371338604920865 1101 62-63 0.18008649420300316 1101 64-65 0.12707430014872045 1101 66-67 0.13037240245287762 1101 68-69 0.2515016157518346 1101 70-71 0.24962689876475253 1101 72-73 0.20634345921882868 1101 74-75 0.17503939865508045 1101 76 0.28029790774567687 1102 1 -0.026151837638828113 1102 2 0.03591062116617394 1102 3 0.03532395734950455 1102 4 0.06316993538063542 1102 5 0.042274224897198565 1102 6 -0.01566672887369691 1102 7 -0.0049164111105497454 1102 8 -0.04423037474280278 1102 9 -0.030312350722155657 1102 10-11 0.023197625818980327 1102 12-13 0.026673280473097805 1102 14-15 0.007268532901939295 1102 16-17 0.10596130934123948 1102 18-19 0.10655000860048602 1102 20-21 0.10992334041376495 1102 22-23 0.019797304551055106 1102 24-25 0.09199659857925724 1102 26-27 0.102696550928421 1102 28-29 0.08020663261849847 1102 30-31 0.12466910077558424 1102 32-33 0.17283393781983847 1102 34-35 0.04877556580587594 1102 36-37 -0.0396446790661642 1102 38-39 0.04879987415448994 1102 40-41 1.2762669204971715 1102 42-43 0.6044899478002179 1102 44-45 0.32507319890503794 1102 46-47 0.7527575462235347 1102 48-49 0.4140628731560696 1102 50-51 0.20288147451938698 1102 52-53 0.25456925394576757 1102 54-55 0.1961507761300183 1102 56-57 0.22936479763107087 1102 58-59 0.276378950105439 1102 60-61 0.2394445662228435 1102 62-63 0.16014976197681108 1102 64-65 0.1609361820776769 1102 66-67 0.2080625764274231 1102 68-69 0.14498850258721063 1102 70-71 0.3112729378324772 1102 72-73 0.2611699412937796 1102 74-75 0.26535659816257606 1102 76 0.35915014085916397 1103 1 -0.02997111874405789 1103 2 0.0026765586734782687 1103 3 0.030193825740454372 1103 4 0.014233836699723668 1103 5 -0.016417180814151777 1103 6 0.08389927146092901 1103 7 0.07918755496900332 1103 8 0.11178342362826044 1103 9 0.13762182820595115 1103 10-11 0.11201222902059982 1103 12-13 0.14130841449727427 1103 14-15 0.040850877269711816 1103 16-17 0.14368646135997665 1103 18-19 0.09018690005605379 1103 20-21 0.1785602489419702 1103 22-23 0.1667571531140979 1103 24-25 0.18211998386858141 1103 26-27 0.16113515022591685 1103 28-29 0.18660487403142412 1103 30-31 0.13897978239157993 1103 32-33 0.16349513756421885 1103 34-35 0.11716846689672167 1103 36-37 0.06920125374485764 1103 38-39 0.12023426622795341 1103 40-41 1.3538796058371076 1103 42-43 0.5820606344434509 1103 44-45 0.28929711867307617 1103 46-47 0.7162755122889024 1103 48-49 0.35035723280201125 1103 50-51 0.18105912637987132 1103 52-53 0.17900528551807326 1103 54-55 0.21743202004789453 1103 56-57 0.1710986648390147 1103 58-59 0.264263219137689 1103 60-61 0.30369564462277765 1103 62-63 0.17828497781832908 1103 64-65 0.2362869066536888 1103 66-67 0.27119890987028583 1103 68-69 0.24732949654585923 1103 70-71 0.2630246039252384 1103 72-73 0.22800564680490965 1103 74-75 0.17444517916607793 1103 76 0.41017258192637485 2116 1 0.005326325733804538 2116 2 -0.01018069011914946 2116 3 0.011418811536145768 2116 4 -1.8536376484235007E-4 2116 5 0.03921226847646864 2116 6 -0.046422121898139324 2116 7 0.05195758304339648 2116 8 0.049981628036604775 2116 9 0.08116779794242035 2116 10-11 0.061209894500791506 2116 12-13 0.09901143165942017 2116 14-15 0.012667728383497945 2116 16-17 0.091329747317765 2116 18-19 0.07836899406350284 2116 20-21 0.11256296029371526 2116 22-23 0.04689485656992787 2116 24-25 0.11172184024631804 2116 26-27 0.11233604091958682 2116 28-29 0.03176290218102196 2116 30-31 0.08421904142802106 2116 32-33 0.1327954239481386 2116 34-35 0.09034947991454345 2116 36-37 0.11794004696355387 2116 38-39 0.05535008356055471 2116 40-41 1.3070743218344987 2116 42-43 0.4790622982274755 2116 44-45 0.29906610544636436 2116 46-47 0.676970181286741 2116 48-49 0.33124643293236034 2116 50-51 0.18259810049460867 2116 52-53 0.18465110631819215 2116 54-55 0.19522551656365295 2116 56-57 0.14246445083858106 2116 58-59 0.16861548180041552 2116 60-61 0.1835822921554069 2116 62-63 0.1692533617440688 2116 64-65 0.24447931967318937 2116 66-67 0.26233714494323124 2116 68-69 0.1801937743821327 2116 70-71 0.20179959724086416 2116 72-73 0.23234057185629098 2116 74-75 0.16889061582322284 2116 76 0.3236163850880871 1117 1 0.01663775199642714 1117 2 0.07052816020842556 1117 3 0.09131492207703928 1117 4 0.050358864075455756 1117 5 0.061611565826446224 1117 6 0.008333311197489479 1117 7 0.05540965778028095 1117 8 0.031401522209712596 1117 9 0.09743843718734979 1117 10-11 0.11633936379360676 1117 12-13 0.08932930034632847 1117 14-15 0.04899906573621848 1117 16-17 0.12429517923386868 1117 18-19 0.13576974384312024 1117 20-21 0.12206790295184788 1117 22-23 0.12407057158434753 1117 24-25 0.05843115592045223 1117 26-27 0.10237389979775458 1117 28-29 0.15973808723322946 1117 30-31 0.1716997964120779 1117 32-33 0.14055916473432717 1117 34-35 0.11562149683360445 1117 36-37 0.20375412924530423 1117 38-39 0.09662773275081094 1117 40-41 1.3761458222421652 1117 42-43 0.544310112728283 1117 44-45 0.2537743895462583 1117 46-47 0.7322762248942993 1117 48-49 0.37400677609375776 1117 50-51 0.22073595378549982 1117 52-53 0.1917794899748273 1117 54-55 0.23527707199551173 1117 56-57 0.11777444045041108 1117 58-59 0.20055612955040658 1117 60-61 0.27966930704648973 1117 62-63 0.22010647338378675 1117 64-65 0.25613823616843945 1117 66-67 0.2673749288072145 1117 68-69 0.23879782266435257 1117 70-71 0.22271697662841206 1117 72-73 0.22939833484047512 1117 74-75 0.20753462079381535 1117 76 0.5249525556817076 2117 1 0.03706648460853046 2117 2 0.058604938633465053 2117 3 0.06261775068477249 2117 4 0.06337822828805173 2117 5 0.03402501724526985 2117 6 -0.025512892997106462 2117 7 0.03789371270676156 2117 8 0.041234663859313514 2117 9 0.10585388544157581 2117 10-11 -4.61578885158076E-4 2117 12-13 -0.05859625571830662 2117 14-15 -0.08745380993532592 2117 16-17 0.06901539630919729 2117 18-19 0.09374996873125241 2117 20-21 0.08304169266907735 2117 22-23 0.11709245911326605 2117 24-25 0.05899708395541836 2117 26-27 0.11797128682123059 2117 28-29 0.14812609198740745 2117 30-31 0.14100789995868723 2117 32-33 0.10083232184329916 2117 34-35 0.097142133223592 2117 36-37 0.2537941672495947 2117 38-39 0.08462363648506255 2117 40-41 1.3081979978784872 2117 42-43 0.5537967691510417 2117 44-45 0.31613575649541303 2117 46-47 0.7905536959261568 2117 48-49 0.3934396883462412 2117 50-51 0.23317195531482326 2117 52-53 0.17262007768026422 2117 54-55 0.2898472614870826 2117 56-57 0.24285320480202444 2117 58-59 0.31777767906143595 2117 60-61 0.3224300709451171 2117 62-63 0.2921309211112657 2117 64-65 -0.15551614767485233 2117 66-67 0.1523528721914289 2117 68-69 0.22485620776268433 2117 70-71 0.36759241077388793 2117 72-73 0.2283560545521084 2117 74-75 0.2502854498649114 2117 76 0.4659216286285357 1116 1 0.0019422414694219015 1116 2 0.007825535734454547 1116 3 0.0032294646634341007 1116 4 -0.010947149814754198 1116 5 0.03535126033195013 1116 6 0.027412325214733357 1116 7 0.0708475015982728 1116 8 -0.005588879429197391 1116 9 0.057193758577277265 1116 10-11 -0.007136403922508805 1116 12-13 -0.05044155466197964 1116 14-15 0.060449947502227985 1116 16-17 0.09817564112584876 1116 18-19 0.14201030266745107 1116 20-21 0.11037112046913222 1116 22-23 0.06887743853713602 1116 24-25 0.06552551702401388 1116 26-27 0.06442829319065879 1116 28-29 0.1770444034340244 1116 30-31 0.08318087707593236 1116 32-33 0.11381808892672751 1116 34-35 0.08532389779070826 1116 36-37 0.23930601490759074 1116 38-39 0.1952764044334785 1116 40-41 1.2597614890058466 1116 42-43 0.5123995580687648 1116 44-45 0.20793230180221656 1116 46-47 0.6373385442918575 1116 48-49 0.35167708710380907 1116 50-51 0.2022755830047842 1116 52-53 0.19767354941102155 1116 54-55 0.22220519443773412 1116 56-57 0.1535864096983559 1116 58-59 0.14775887221495054 1116 60-61 0.15049083606268 1116 62-63 0.17517363296678923 1116 64-65 -0.17077020392501652 1116 66-67 0.13127020070182027 1116 68-69 0.174165138065959 1116 70-71 0.20401852018124345 1116 72-73 0.23638241690171213 1116 74-75 0.14946027490226754 1116 76 0.3768976187729578 2118 1 0.01630792536199266 2118 2 0.00983420340797636 2118 3 0.03837556749355997 2118 4 0.0538617673826991 2118 5 0.04404421456401053 2118 6 0.10510948195516079 2118 7 0.09731312639306822 2118 8 0.089153716933005 2118 9 0.14971657688445106 2118 10-11 0.1005351437825226 2118 12-13 0.07615884588960142 2118 14-15 0.10225308625066276 2118 16-17 0.12080467126571648 2118 18-19 0.16650091268780898 2118 20-21 0.08751965287441266 2118 22-23 0.14287228389796525 2118 24-25 0.12976545398638706 2118 26-27 0.18221097754287996 2118 28-29 0.21472169675597286 2118 30-31 0.21964239785864947 2118 32-33 0.1465431529493202 2118 34-35 0.1763326639605154 2118 36-37 0.1909558277646326 2118 38-39 0.16487930022440622 2118 40-41 1.296585476135668 2118 42-43 0.5280575132857876 2118 44-45 0.2550713128994673 2118 46-47 0.6824905568993884 2118 48-49 0.3422544395235434 2118 50-51 0.13503282248209558 2118 52-53 0.20812663234489293 2118 54-55 0.12791469604650274 2118 56-57 0.17415703153826456 2118 58-59 0.08263969170408103 2118 60-61 0.14054369573830172 2118 62-63 0.17115334273105276 2118 64-65 0.14112273060778335 2118 66-67 0.1628710937348501 2118 68-69 0.14861421548403797 2118 70-71 0.23618199119049876 2118 72-73 0.22254992625887127 2118 74-75 0.15934027358898817 2118 76 0.36831333878083683 1119 1 0.007176425962597932 1119 2 0.02009398138228846 1119 3 0.018636137473890813 1119 4 0.0016530605965243694 1119 5 0.01415093737510631 1119 6 -0.004829533581357737 1119 7 0.012339790595717659 1119 8 0.020116202247891124 1119 9 0.06308233846424116 1119 10-11 0.06463945097525681 1119 12-13 0.08055398429265637 1119 14-15 0.05503764741332873 1119 16-17 0.12191166467886205 1119 18-19 0.09960296926343659 1119 20-21 0.0641439252188789 1119 22-23 0.0699273745166451 1119 24-25 0.08110394415891164 1119 26-27 0.1403704545549047 1119 28-29 0.12168095464633666 1119 30-31 0.18359011886132492 1119 32-33 0.1457399268448043 1119 34-35 0.10533971941413967 1119 36-37 0.11335936803153857 1119 38-39 0.2156843413427154 1119 40-41 1.2680559343111497 1119 42-43 0.49344982026731543 1119 44-45 0.2551821937421579 1119 46-47 0.6770243953565611 1119 48-49 0.2991592470000768 1119 50-51 0.16102910005380977 1119 52-53 0.21237640464316598 1119 54-55 0.2056626269307742 1119 56-57 0.17608622620014103 1119 58-59 0.1913596779759814 1119 60-61 0.1714929809528627 1119 62-63 0.19775451489996243 1119 64-65 0.19999600304466014 1119 66-67 0.1923726579467342 1119 68-69 0.11473846802835652 1119 70-71 0.2720844490491814 1119 72-73 0.16931227165269291 1119 74-75 0.21689056597120526 1119 76 0.16266435584856964 2119 1 -0.06616630144213076 2119 2 -0.002536930612642152 2119 3 0.0404374231636595 2119 4 -0.012117614060599635 2119 5 0.021621534151641697 2119 6 0.04505793556341331 2119 7 0.008395600619870436 2119 8 0.044050624149498674 2119 9 0.10925265935868111 2119 10-11 0.04434957677221263 2119 12-13 0.09149389743599556 2119 14-15 0.06681494192076798 2119 16-17 -0.03138605038377307 2119 18-19 0.012377201347604228 2119 20-21 -0.03425869764105016 2119 22-23 0.056918675650543094 2119 24-25 0.08015205192452868 2119 26-27 0.029504791975867306 2119 28-29 0.11085571737452682 2119 30-31 0.10624033600297622 2119 32-33 0.0879091323307648 2119 34-35 0.10648730313577204 2119 36-37 0.1445640751873114 2119 38-39 0.1373019806367708 2119 40-41 1.3025133111872123 2119 42-43 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-0.18044014861762747 2104 54-55 -0.3518958522055087 2104 56-57 -0.20375022176532553 2104 58-59 -0.2037082301594637 2104 60-61 -0.21152785761880466 2104 62-63 -0.18713548978069383 2104 64-65 -0.2658108678428519 2104 66-67 -0.3296492289362192 2104 68-69 -0.3053647384115976 2104 70-71 -0.22681811215711178 2104 72-73 -0.17990613684830237 2104 74-75 -0.253704495747634 2104 76 -0.29457962990058917 2111 1 -0.1387565606589618 2111 2 -0.10939917659287346 2111 3 -0.0422347870381401 2111 4 -0.045006545374505436 2111 5 -0.08262450264498966 2111 6 0.008300550075595936 2111 7 -0.014369661778836473 2111 8 -0.010171371805981266 2111 9 -0.15078191702755817 2111 10-11 -0.07984258025690139 2111 12-13 -0.0633287660063715 2111 14-15 0.011645984303356727 2111 16-17 -0.048422875295742074 2111 18-19 -0.06317732893678141 2111 20-21 -0.015620858771249857 2111 22-23 -0.11729540096903435 2111 24-25 -0.10589489135323049 2111 26-27 -0.008340441573835733 2111 28-29 -0.025896541757454372 2111 30-31 -0.07899381471894884 2111 32-33 -0.12084120353392791 2111 34-35 -0.06576938941014987 2111 36-37 -0.04176481766123885 2111 38-39 -0.0879329478770714 2111 40-41 1.084303964956682 2111 42-43 -0.11599061082152673 2111 44-45 -0.19710155489005388 2111 46-47 0.3205005409192978 2111 48-49 -0.15714330205156557 2111 50-51 -0.37631021203772264 2111 52-53 -0.3211727892607499 2111 54-55 -0.28105465895187365 2111 56-57 -0.3602503617274735 2111 58-59 -0.3100858325983751 2111 60-61 -0.3340942774773765 2111 62-63 -0.4272963765567752 2111 64-65 -0.29730284177635724 2111 66-67 -0.1838268260253102 2111 68-69 -0.17408069210014077 2111 70-71 -0.19703378950046613 2111 72-73 -0.4816490894808183 2111 74-75 -0.7364343718086062 2111 76 -0.06593708193388181 2110 1 0.02562825199708385 2110 2 -0.05372415401620856 2110 3 -0.05155894126550464 2110 4 -0.0070761617576451386 2110 5 -0.032371647737328146 2110 6 0.04030897116312104 2110 7 0.031651928183670464 2110 8 0.03521652679888376 2110 9 -0.06054102765944691 2110 10-11 -0.09745693950716117 2110 12-13 -0.06644440262318341 2110 14-15 -0.03128788612296063 2110 16-17 -0.09339638797654004 2110 18-19 -0.051708193511068146 2110 20-21 -0.2706901824206298 2110 22-23 -0.12949663451971816 2110 24-25 -0.13888553404510873 2110 26-27 0.002953091270555319 2110 28-29 -0.1120680927829838 2110 30-31 -0.043242395035569814 2110 32-33 -0.06651757863852481 2110 34-35 -0.04620663644692513 2110 36-37 -0.13146232234139887 2110 38-39 -0.0049871194461843515 2110 40-41 0.9416442922533648 2110 42-43 -1.1950560276119404E-4 2110 44-45 -0.25725480120196664 2110 46-47 0.01638829089678495 2110 48-49 -0.19374086169936078 2110 50-51 -0.27656504487619316 2110 52-53 -0.32861372730479843 2110 54-55 -0.32264564621814174 2110 56-57 -0.2989163849073151 2110 58-59 -0.41752311617977966 2110 60-61 -0.2027390633315207 2110 62-63 -0.3954573793714573 2110 64-65 -0.3188182833880262 2110 66-67 -0.6788664769450108 2110 68-69 -0.6681771525835316 2110 70-71 -0.4812811717561303 2110 72-73 -0.65244115470054 2110 74-75 -0.34433893683524985 2110 76 -0.36461533437737614 2109 1 0.03578752891341708 2109 2 -0.043604145722923704 2109 3 -0.06765423537827076 2109 4 -0.039381097754400685 2109 5 -0.0326164042234538 2109 6 0.04107397926967593 2109 7 -0.0360479464180159 2109 8 0.04105072222321837 2109 9 -0.0803242679340812 2109 10-11 -0.1410838802109211 2109 12-13 -0.136246109140707 2109 14-15 -0.0847010902474139 2109 16-17 -0.23348497214979602 2109 18-19 -0.1592253376078574 2109 20-21 -0.17837449097088154 2109 22-23 -0.13815110091445604 2109 24-25 -0.23529780356096808 2109 26-27 -0.17768833824872132 2109 28-29 -0.21247277477091586 2109 30-31 -0.15879688874159825 2109 32-33 -0.33738746854959345 2109 34-35 -0.07855710406629868 2109 36-37 -0.38586747801191024 2109 38-39 -0.14267489992674598 2109 40-41 -10.773075347452789 2109 42-43 -1.0214917802950083 2109 44-45 -0.2897691442394361 2109 46-47 0.27507791462038966 2109 48-49 -0.0646259365026296 2109 50-51 -0.11261946580847138 2109 52-53 -0.07967617025578733 2109 54-55 -0.2511321428068385 2109 56-57 -0.1551574908715807 2109 58-59 -0.1621926887757894 2109 60-61 -0.2638461126718141 2109 62-63 -0.12146040835785499 2109 64-65 -0.1806952820725698 2109 66-67 -0.24557103411866876 2109 68-69 -0.535256552202398 2109 70-71 -0.33207681092558516 2109 72-73 -0.1351974646918208 2109 74-75 -0.3170175425977959 2109 76 -0.15404660329797792 2108 1 0.04664861869251524 2108 2 -0.01872995152759671 2108 3 0.008910928054184808 2108 4 0.02457767970834368 2108 5 -0.03251596984676297 2108 6 0.018521961669684117 2108 7 -0.1528176837641979 2108 8 -0.09941356336022267 2108 9 -0.09962519051263996 2108 10-11 -0.11554372087628195 2108 12-13 -0.19038629131799922 2108 14-15 -0.139829487752813 2108 16-17 -0.35064390266146717 2108 18-19 -0.40830940157938045 2108 20-21 -0.45323669835122615 2108 22-23 -0.21749887800142886 2108 24-25 -0.6424518772222925 2108 26-27 -0.5543262508958264 2108 28-29 -0.929759442517593 2108 30-31 -0.46067889037562537 2108 32-33 -0.8683087520515471 2108 34-35 -0.5317462021655928 2108 36-37 -0.959904379242623 2108 38-39 -0.3779442990137696 2108 40-41 -7.547204049367544 2108 42-43 -0.5680939682511337 2108 44-45 -0.2388302779515996 2108 46-47 0.3724506755749388 2108 48-49 0.0572584672731935 2108 50-51 -0.16433154139756567 2108 52-53 -0.3553461535666358 2108 54-55 -0.3454673811278184 2108 56-57 -0.25280336005584303 2108 58-59 -0.22181709765854407 2108 60-61 -0.49329637105338264 2108 62-63 -0.3377572280208838 2108 64-65 -0.1672558323733071 2108 66-67 -0.20560285134689593 2108 68-69 -0.14424067401328955 2108 70-71 -0.12293066401358743 2108 72-73 -0.25696335431020856 2108 74-75 -0.3040566893850638 2108 76 -0.8413677867000686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 2.0 16 2.0 17 11.0 18 42.0 19 66.0 20 161.0 21 245.0 22 378.0 23 525.0 24 763.0 25 1095.0 26 1746.0 27 2609.0 28 3790.0 29 5791.0 30 8486.0 31 12592.0 32 18656.0 33 27444.0 34 41836.0 35 67188.0 36 147078.0 37 1092240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.71968044602431 27.042318008692394 8.454528259350743 23.783473285932548 2 16.86717892272676 24.315528142791436 37.75244338323514 21.064849551246663 3 19.737329758847864 25.98009278679348 31.172286523719823 23.110290930638836 4 14.580034018567131 16.970546788791044 39.19512656456444 29.254292628077394 5 13.942796599818392 34.825269220595125 36.35415045363906 14.877783725947427 6 27.5067405480521 37.098036150136764 19.448164958642383 15.947058343168752 7 23.429956579912574 34.144130122066215 23.29196990117585 19.13394339684536 8 21.12257083769849 39.34211692643572 22.647403903131536 16.88790833273425 9 20.823634598432243 19.181474468276676 25.220572787798545 34.774318145492536 10-11 23.87588318105011 26.679169455598235 29.212659318079186 20.232288045272472 12-13 21.54923374468765 25.441930780521616 29.662599188830967 23.34623628595977 14-15 20.871863629796465 29.21049564228716 24.861856280278374 25.055784447637997 16-17 20.01072066456953 32.779583555226424 27.390041647269197 19.819654132934843 18-19 19.55331262253559 31.155186505363474 29.964711145791966 19.32678972630897 20-21 19.87922501320889 29.398491150217033 30.112713549565974 20.609570287008104 22-23 20.313425887473503 29.825119159209546 29.568688679857647 20.292766273459307 24-25 20.228833143604557 30.36062891773635 29.459074072393797 19.951463866265293 26-27 19.799692478853558 30.499767230362373 29.76212827526423 19.938412015519837 28-29 19.668964583419125 30.164467278591406 30.15246236774532 20.014105770244154 30-31 19.93656242169762 29.845359997263998 29.998248120568388 20.21982946046999 32-33 20.045548865221843 30.13672337125815 29.64574345645114 20.17198430706887 34-35 19.906201164615943 30.032134075311273 29.93225600891155 20.129408751161233 36-37 19.676991122647614 30.227632652519947 30.24857145050731 19.846804774325125 38-39 19.717158716786702 29.90852537119254 30.244313894916548 20.130002017104207 40-41 19.773553879366002 29.814230984256117 30.132465815667388 20.279749320710494 42-43 19.977986343715955 29.729010076447555 30.085388418192466 20.20761516164403 44-45 19.48082948350267 29.502556976214223 30.663787814596716 20.35282572568639 46-47 19.481492545438936 29.263889577154934 30.995353680726605 20.259264196679524 48-49 19.213824911167148 29.280954697514634 31.054959458997295 20.45026093232092 50-51 19.04198717568419 29.482316138159774 30.94011015203661 20.53558653411942 52-53 18.983916909265908 29.27491734409495 30.996121436652807 20.74504430998634 54-55 18.788243842074 29.088178164044315 31.242989864923814 20.880588128957868 56-57 18.488016376931864 29.120703096918017 31.262079069088962 21.129201457061157 58-59 18.32950967616753 29.041589338522435 31.36443489325052 21.26446609205952 60-61 18.33760601138931 28.84033259186723 31.238453125359882 21.58360827138357 62-63 18.058736120194286 28.539791044755287 31.42166760774931 21.97980522730112 64-65 18.177179920809465 28.386484145491142 31.001740014112748 22.434595919586638 66-67 18.265646342306074 28.637016863409936 30.44159226995415 22.655744524329833 68-69 17.970269698697678 28.64099523502754 30.43262348481623 22.956111581458554 70-71 17.918655561658827 28.487897723743274 30.395875894348407 23.197570820249496 72-73 17.852872837981863 28.36421922363125 30.30981743462035 23.473090503766542 74-75 18.19232565135366 28.415274992723766 29.68367757880491 23.708721777117663 76 18.420209569449455 28.287059753047817 29.22511790288167 24.06761277462106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 43.0 1 23.5 2 9.0 3 12.0 4 10.0 5 11.5 6 13.0 7 21.0 8 29.0 9 63.0 10 171.0 11 454.5 12 664.0 13 882.5 14 1347.0 15 2058.0 16 3194.5 17 3866.0 18 5138.5 19 7670.5 20 10514.5 21 12099.0 22 13964.5 23 18656.5 24 23711.5 25 25940.0 26 28802.0 27 34079.0 28 38910.0 29 41326.0 30 43884.5 31 48332.5 32 51508.0 33 52794.0 34 54552.5 35 58007.0 36 61280.5 37 62858.0 38 63621.0 39 65108.0 40 66328.5 41 66684.0 42 66543.0 43 66695.5 44 66213.5 45 64254.5 46 62930.0 47 61187.0 48 57366.0 49 53279.5 50 51271.0 51 49573.0 52 45252.0 53 40104.0 54 37579.0 55 34872.5 56 29522.5 57 24095.0 58 21311.0 59 19215.0 60 14994.5 61 11121.0 62 9372.0 63 8068.5 64 5818.5 65 4086.0 66 2750.0 67 2200.0 68 1850.5 69 1225.5 70 801.0 71 652.0 72 514.0 73 328.0 74 223.5 75 167.0 76 132.0 77 80.5 78 45.0 79 26.0 80 22.5 81 14.0 82 4.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1432747.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.62204111951701 #Duplication Level Percentage of deduplicated Percentage of total 1 92.75683703914669 81.27543389160448 2 5.587323499512943 9.791453788447333 3 0.7527856437941091 1.978818439041285 4 0.2830865258849831 0.9921847684590082 5 0.15811032949346532 0.6926974896148399 6 0.07206956153030425 0.37889292503243277 7 0.06194671261891659 0.37995281802195285 8 0.06884948409086906 0.48261858608541286 9 0.041990283568518826 0.3311346918114842 >10 0.21025155267017215 3.2243160265644324 >50 0.005486839630369334 0.29600562109036394 >100 0.0012625280586398327 0.17649095422708286 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.1877595974725473E-4 0.0 0.0 2 4.1877595974725473E-4 0.0 0.0 3 4.1877595974725473E-4 0.0 0.0 4 4.1877595974725473E-4 0.0 6.979599329120912E-5 5 4.1877595974725473E-4 0.0 6.979599329120912E-5 6 4.1877595974725473E-4 0.0 6.979599329120912E-5 7 4.1877595974725473E-4 0.0 6.979599329120912E-5 8 4.1877595974725473E-4 0.0 1.3959198658241824E-4 9 4.885719530384638E-4 0.0 1.3959198658241824E-4 10 5.58367946329673E-4 0.0 2.0938797987362737E-4 11 5.58367946329673E-4 0.0 2.0938797987362737E-4 12 5.58367946329673E-4 0.0 2.0938797987362737E-4 13 5.58367946329673E-4 0.0 2.0938797987362737E-4 14 6.281639396208821E-4 0.0 2.0938797987362737E-4 15 6.281639396208821E-4 0.0 2.0938797987362737E-4 16 6.281639396208821E-4 0.0 2.0938797987362737E-4 17 6.281639396208821E-4 6.979599329120912E-5 2.0938797987362737E-4 18 6.281639396208821E-4 6.979599329120912E-5 2.0938797987362737E-4 19 6.281639396208821E-4 6.979599329120912E-5 2.791839731648365E-4 20 6.281639396208821E-4 2.0938797987362737E-4 4.1877595974725473E-4 21 6.281639396208821E-4 2.0938797987362737E-4 4.1877595974725473E-4 22 6.281639396208821E-4 2.0938797987362737E-4 4.885719530384638E-4 23 6.281639396208821E-4 2.0938797987362737E-4 6.979599329120913E-4 24 6.281639396208821E-4 2.0938797987362737E-4 8.375519194945095E-4 25 6.281639396208821E-4 2.0938797987362737E-4 0.0010469398993681368 26 6.281639396208821E-4 2.0938797987362737E-4 0.0018844918188626464 27 6.281639396208821E-4 2.0938797987362737E-4 0.013889402664950616 28 6.281639396208821E-4 2.0938797987362737E-4 0.036014732538263906 29 6.281639396208821E-4 2.0938797987362737E-4 0.06142047409626403 30 6.979599329120913E-4 2.0938797987362737E-4 0.08382498794274217 31 6.979599329120913E-4 2.0938797987362737E-4 0.10134378225883565 32 6.979599329120913E-4 2.791839731648365E-4 0.1247254400113907 33 6.979599329120913E-4 4.885719530384638E-4 0.1583671087777535 34 6.979599329120913E-4 4.885719530384638E-4 0.19745286502083062 35 7.677559262033004E-4 4.885719530384638E-4 0.25119577985506164 36 7.677559262033004E-4 4.885719530384638E-4 0.35079466228161704 37 7.677559262033004E-4 4.885719530384638E-4 0.5072074832472168 38 7.677559262033004E-4 4.885719530384638E-4 0.6840007342538494 39 7.677559262033004E-4 4.885719530384638E-4 0.8704956283279602 40 7.677559262033004E-4 4.885719530384638E-4 1.1009619981755328 41 7.677559262033004E-4 4.885719530384638E-4 1.3691880003936494 42 7.677559262033004E-4 4.885719530384638E-4 1.6732891431634476 43 7.677559262033004E-4 4.885719530384638E-4 2.011869506619103 44 7.677559262033004E-4 5.58367946329673E-4 2.413615244003303 45 7.677559262033004E-4 5.58367946329673E-4 2.8774794154166785 46 8.375519194945095E-4 5.58367946329673E-4 3.3597697290589337 47 8.375519194945095E-4 5.58367946329673E-4 3.8583225091380404 48 8.375519194945095E-4 5.58367946329673E-4 4.34898834197524 49 8.375519194945095E-4 5.58367946329673E-4 4.816761089012924 50 9.073479127857186E-4 5.58367946329673E-4 5.321525712494949 51 9.073479127857186E-4 5.58367946329673E-4 5.837318102916984 52 9.073479127857186E-4 5.58367946329673E-4 6.434702009496442 53 9.073479127857186E-4 5.58367946329673E-4 7.055397777835165 54 9.073479127857186E-4 5.58367946329673E-4 7.74198096384079 55 9.073479127857186E-4 5.58367946329673E-4 8.458716018948216 56 9.073479127857186E-4 5.58367946329673E-4 9.185222513116413 57 9.073479127857186E-4 5.58367946329673E-4 9.890860005290536 58 0.0010469398993681368 6.281639396208821E-4 10.549175814013221 59 0.0010469398993681368 6.281639396208821E-4 11.175385465821948 60 0.0010469398993681368 6.281639396208821E-4 11.837889034142107 61 0.0010469398993681368 6.281639396208821E-4 12.500671786435428 62 0.0010469398993681368 6.281639396208821E-4 13.228783588449321 63 0.0010469398993681368 6.281639396208821E-4 13.965201113664868 64 0.0010469398993681368 6.281639396208821E-4 14.784955054870121 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTG 230 6.0827006E-9 18.260868 5 CCGCTCC 260 1.4624675E-9 17.5 9 GTGTAGC 360 0.0 17.499998 1 CGTACTA 825 0.0 16.121212 70 CCCTGCG 155 4.0560175E-4 15.806452 9 AGGGGTG 290 6.2245817E-9 15.689655 5 TAGACCC 135 0.002947079 15.555555 4 TACTAGG 180 7.1528186E-5 15.555554 2 GTAGCAC 390 5.456968E-12 15.256411 3 ACCGCTC 325 1.722583E-9 15.076923 8 TACACTG 475 0.0 14.736843 5 GTATTAG 285 8.540337E-8 14.736841 1 TGTAGCA 550 0.0 14.636364 2 ACTGTGC 360 4.656613E-10 14.583332 8 TATACTG 650 0.0 14.538462 5 AGCACCG 420 2.1827873E-11 14.166666 5 GTCCAGA 420 2.1827873E-11 14.166666 1 TAGCGTG 225 3.0341129E-5 14.0 5 CGTACGT 150 0.005936178 14.0 10 GGCTAGA 175 0.0010181608 14.0 1 >>END_MODULE