##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n01 ve08.3410000000c760.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1259515 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.70750487290743 34.0 34.0 34.0 34.0 34.0 2 33.73808727962748 34.0 34.0 34.0 34.0 34.0 3 33.76903728816251 34.0 34.0 34.0 34.0 34.0 4 33.78270445369845 34.0 34.0 34.0 34.0 34.0 5 33.79280119728626 34.0 34.0 34.0 34.0 34.0 6 37.54137743496505 38.0 38.0 38.0 37.0 38.0 7 37.57570414008567 38.0 38.0 38.0 37.0 38.0 8 37.47471367947186 38.0 38.0 38.0 37.0 38.0 9 37.44327697566126 38.0 38.0 38.0 37.0 38.0 10-11 37.44386251850911 38.0 38.0 38.0 37.0 38.0 12-13 37.369400920195474 38.0 38.0 38.0 37.0 38.0 14-15 37.430587170458466 38.0 38.0 38.0 37.0 38.0 16-17 37.37013612382544 38.0 38.0 38.0 37.0 38.0 18-19 37.39136056339146 38.0 38.0 38.0 37.0 38.0 20-21 37.33460260497096 38.0 38.0 38.0 37.0 38.0 22-23 37.39856571775644 38.0 38.0 38.0 37.0 38.0 24-25 37.316611949837835 38.0 38.0 38.0 37.0 38.0 26-27 37.27553026363323 38.0 38.0 38.0 37.0 38.0 28-29 37.19015573454861 38.0 38.0 38.0 36.0 38.0 30-31 37.2261632453762 38.0 38.0 38.0 37.0 38.0 32-33 37.16890231557385 38.0 38.0 38.0 36.0 38.0 34-35 37.215248329714214 38.0 38.0 38.0 36.5 38.0 36-37 37.1093333545055 38.0 38.0 38.0 36.0 38.0 38-39 37.181447223732945 38.0 38.0 38.0 36.0 38.0 40-41 36.00001389423707 38.0 37.5 38.0 32.5 38.0 42-43 36.654486449149076 38.0 37.0 38.0 34.5 38.0 44-45 36.862155274053904 38.0 38.0 38.0 35.0 38.0 46-47 36.401437458069175 38.0 37.5 38.0 34.0 38.0 48-49 36.75884249095882 38.0 37.0 38.0 34.5 38.0 50-51 36.83165067506143 38.0 38.0 38.0 35.5 38.0 52-53 36.843151530549456 38.0 38.0 38.0 35.5 38.0 54-55 36.835575995522085 38.0 38.0 38.0 35.0 38.0 56-57 36.83634811812483 38.0 38.0 38.0 35.0 38.0 58-59 36.79250346363481 38.0 38.0 38.0 35.0 38.0 60-61 36.79306320289953 38.0 38.0 38.0 35.0 38.0 62-63 36.79930647908124 38.0 38.0 38.0 35.0 38.0 64-65 36.72020301465247 38.0 37.5 38.0 34.5 38.0 66-67 36.69200843181701 38.0 37.0 38.0 35.0 38.0 68-69 36.6809025696399 38.0 37.0 38.0 34.5 38.0 70-71 36.5943327391893 38.0 37.0 38.0 34.0 38.0 72-73 36.64022778609227 38.0 37.0 38.0 34.0 38.0 74-75 36.63795111610421 38.0 37.0 38.0 34.0 38.0 76 35.83022671425112 38.0 37.0 38.0 32.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0806790691593875 1101 2 0.11359279517837706 1101 3 0.08249644464039108 1101 4 0.043059534794181786 1101 5 0.07293450961442005 1101 6 0.16930499951425304 1101 7 0.06892637238314592 1101 8 0.15031028334116314 1101 9 0.0986161797639511 1101 10-11 0.15248688814848066 1101 12-13 0.20073380364892301 1101 14-15 0.17056343829980847 1101 16-17 0.18610721944656206 1101 18-19 0.20154250405351348 1101 20-21 0.19676985014540804 1101 22-23 0.14369401539681803 1101 24-25 0.16231257472313132 1101 26-27 0.2057135756744657 1101 28-29 0.27055396904420803 1101 30-31 0.2435314253447416 1101 32-33 0.3059523420237866 1101 34-35 0.21396011998810138 1101 36-37 0.16978342092725995 1101 38-39 0.1824216736794071 1101 40-41 1.4368284457306046 1101 42-43 0.7039123995298553 1101 44-45 0.37430081753657163 1101 46-47 0.8849511280489963 1101 48-49 0.49945800497786763 1101 50-51 0.3072483116312128 1101 52-53 0.3958233899802792 1101 54-55 0.36642693967756657 1101 56-57 0.3417752633715665 1101 58-59 0.33171621505915283 1101 60-61 0.33427335179160167 1101 62-63 0.36180247297265566 1101 64-65 0.3194526635776711 1101 66-67 0.3593717667871559 1101 68-69 0.42130582073397704 1101 70-71 0.45822424992079647 1101 72-73 0.4993111154886165 1101 74-75 0.47963069248034174 1101 76 0.4762161358044068 1102 1 0.011183029030824798 1102 2 0.07902129888874754 1102 3 0.10103917395309736 1102 4 0.059211558309336 1102 5 0.05535357383664774 1102 6 0.05300641062890321 1102 7 0.10933638172473792 1102 8 0.06866915629279902 1102 9 0.13643599613369162 1102 10-11 0.17569122684142968 1102 12-13 0.18405495656703152 1102 14-15 0.10424105776228743 1102 16-17 0.21839776321279913 1102 18-19 0.2220728431793546 1102 20-21 0.21464412863343085 1102 22-23 0.14157965546841922 1102 24-25 0.18910744703131854 1102 26-27 0.22775223724663363 1102 28-29 0.24469765209558858 1102 30-31 0.25944820158804305 1102 32-33 0.23747846758169544 1102 34-35 0.20839457698927077 1102 36-37 0.07707919524563778 1102 38-39 0.18946079962254458 1102 40-41 1.4033274756545424 1102 42-43 0.8035423080350341 1102 44-45 0.49490027453264673 1102 46-47 1.0380263669017182 1102 48-49 0.618008263145363 1102 50-51 0.34215648668784127 1102 52-53 0.5132269305518165 1102 54-55 0.42861751768085554 1102 56-57 0.4602885053239163 1102 58-59 0.5187235863496866 1102 60-61 0.4564101789531989 1102 62-63 0.40841676110608915 1102 64-65 0.4386218344325101 1102 66-67 0.5349256616505826 1102 68-69 0.41110284861845514 1102 70-71 0.5422853267542891 1102 72-73 0.5614740981586692 1102 74-75 0.47116147666434216 1102 76 0.6975219155704124 1103 1 0.05562721272730897 1103 2 0.022317017316233034 1103 3 0.08763839124721784 1103 4 0.06830246596843637 1103 5 0.024331986425465857 1103 6 0.10365234457769645 1103 7 0.12471408897106073 1103 8 0.1370932362164936 1103 9 0.1610686460063704 1103 10-11 0.18075800159424915 1103 12-13 0.2014700678808623 1103 14-15 0.12854473208255257 1103 16-17 0.2246462118499295 1103 18-19 0.18833121266390407 1103 20-21 0.23854759858328833 1103 22-23 0.18230423012724373 1103 24-25 0.17832984568715915 1103 26-27 0.21613666370542006 1103 28-29 0.2680140453863942 1103 30-31 0.24900544262541757 1103 32-33 0.261281886438951 1103 34-35 0.22769490918567925 1103 36-37 0.165519439267932 1103 38-39 0.22624979505760479 1103 40-41 1.469402303949387 1103 42-43 0.6896524680285836 1103 44-45 0.3973718397050092 1103 46-47 0.9485475760008626 1103 48-49 0.4758330618304356 1103 50-51 0.33219394146994574 1103 52-53 0.29615594617191476 1103 54-55 0.25098690237727084 1103 56-57 0.30766897005868543 1103 58-59 0.37561548045507465 1103 60-61 0.4091617296860761 1103 62-63 0.3728997650989996 1103 64-65 0.4088144050266109 1103 66-67 0.3607557274094688 1103 68-69 0.4343182064493618 1103 70-71 0.4255365691045725 1103 72-73 0.5180137305187458 1103 74-75 0.4310101581476573 1103 76 0.41094007039043845 2116 1 0.03878174974426685 2116 2 0.0051856105358041304 2116 3 0.027530924299419723 2116 4 -0.0058837133936719965 2116 5 0.0574800290187909 2116 6 0.008789622821502974 2116 7 0.09838140802254713 2116 8 0.13661655813309181 2116 9 0.15744374878219958 2116 10-11 0.14962464900883532 2116 12-13 0.13754791521272836 2116 14-15 0.07923960388389162 2116 16-17 0.16489247030528276 2116 18-19 0.14271308855610698 2116 20-21 0.1948383936520699 2116 22-23 0.10546179997697891 2116 24-25 0.1770109417441006 2116 26-27 0.17811260155063025 2116 28-29 0.12089816847527146 2116 30-31 0.1413154460554864 2116 32-33 0.19858358583415736 2116 34-35 0.1475712187893521 2116 36-37 0.1549705721980672 2116 38-39 0.07390370028694093 2116 40-41 1.4006320325203134 2116 42-43 0.5314068514492689 2116 44-45 0.41292440796581076 2116 46-47 0.7851448826435856 2116 48-49 0.44480626823456504 2116 50-51 0.2655297864007622 2116 52-53 0.2583065398757256 2116 54-55 0.29384867960467176 2116 56-57 0.2189636554763723 2116 58-59 0.27388947703509814 2116 60-61 0.3313241709840469 2116 62-63 0.2780947365235349 2116 64-65 0.40444044224280873 2116 66-67 0.3642693366948535 2116 68-69 0.292914584956975 2116 70-71 0.25934190411594216 2116 72-73 0.26170531223246485 2116 74-75 0.3555127987929083 2116 76 0.6057075195826087 1117 1 3.151334825020058E-4 1117 2 0.008830035802269265 1117 3 0.04435944172721662 1117 4 0.03871216354350793 1117 5 0.07405751495961965 1117 6 0.08437683423329645 1117 7 0.1405383909784348 1117 8 0.174292527128749 1117 9 0.2161962492972762 1117 10-11 0.1799098888277939 1117 12-13 0.17718878182171238 1117 14-15 0.14176961084879736 1117 16-17 0.19799213215755884 1117 18-19 0.24109449993320453 1117 20-21 0.2091629856749435 1117 22-23 0.2087372277731987 1117 24-25 0.2391887441633429 1117 26-27 0.2524729181294063 1117 28-29 0.3278188290408721 1117 30-31 0.2253660719407975 1117 32-33 0.21821311917901198 1117 34-35 0.23200391898814132 1117 36-37 0.3049865811392394 1117 38-39 0.2241498867564573 1117 40-41 1.414607085541114 1117 42-43 0.6725120265864604 1117 44-45 0.44032106092112855 1117 46-47 0.8273492573964347 1117 48-49 0.4728429652434798 1117 50-51 0.3402577127991435 1117 52-53 0.407187850432571 1117 54-55 0.44837996258097235 1117 56-57 0.4134743290968643 1117 58-59 0.3726072956922124 1117 60-61 0.4437313983075626 1117 62-63 0.3772303791374867 1117 64-65 0.4540360949607347 1117 66-67 0.4135065521990384 1117 68-69 0.43477806804473573 1117 70-71 0.4601006510972212 1117 72-73 0.34893608841053236 1117 74-75 0.359685550477451 1117 76 0.7789061530681707 2117 1 0.05597517355592174 2117 2 0.09467433688546834 2117 3 0.07198839947702851 2117 4 0.056016252598951155 2117 5 0.06388848125450153 2117 6 0.0614447196259178 2117 7 0.08949505033869798 2117 8 0.07976380377193948 2117 9 0.17939521120972302 2117 10-11 -0.003637482626494659 2117 12-13 -0.007428148825781022 2117 14-15 -0.03734372789983098 2117 16-17 0.17895143985339956 2117 18-19 0.17668504775607374 2117 20-21 0.20491142441535004 2117 22-23 0.16239322988474214 2117 24-25 0.09918045796000285 2117 26-27 0.16248516386938405 2117 28-29 0.20470602395872817 2117 30-31 0.24145070338479968 2117 32-33 0.2732122861062436 2117 34-35 0.1800839967697172 2117 36-37 0.36970856253542905 2117 38-39 0.21226928494750297 2117 40-41 1.40345355745648 2117 42-43 0.7408391079033407 2117 44-45 0.4929914125840682 2117 46-47 0.9892233016377929 2117 48-49 0.5750982920075813 2117 50-51 0.36899364576213856 2117 52-53 0.38788548032689363 2117 54-55 0.44373658763409907 2117 56-57 0.45907543542374185 2117 58-59 0.5086648329385355 2117 60-61 0.5164231091281337 2117 62-63 0.3766385967421826 2117 64-65 -0.04778983024799999 2117 66-67 0.2811700732902267 2117 68-69 0.43148901241804083 2117 70-71 0.5357912676359007 2117 72-73 0.4749678898403573 2117 74-75 0.4737268063653062 2117 76 0.8729137079796985 1116 1 0.022619229602561575 1116 2 0.040893632936580104 1116 3 0.056330591186281254 1116 4 0.05049570133059689 1116 5 0.07938703831672456 1116 6 0.11391997426792244 1116 7 0.10261984776931854 1116 8 0.09587069104789947 1116 9 0.19446088298803943 1116 10-11 0.05706393530188336 1116 12-13 0.056336840913338904 1116 14-15 0.1635529607884294 1116 16-17 0.19822316881855073 1116 18-19 0.2016857132777048 1116 20-21 0.19541188325005265 1116 22-23 0.18718428571644097 1116 24-25 0.1513119321721632 1116 26-27 0.21321201222833253 1116 28-29 0.32718317365876004 1116 30-31 0.2906519470926554 1116 32-33 0.2658853472047582 1116 34-35 0.21807840923182198 1116 36-37 0.31669773084509956 1116 38-39 0.23959212723752188 1116 40-41 1.395619558219714 1116 42-43 0.6299153741540238 1116 44-45 0.3804303771414297 1116 46-47 0.800787508123129 1116 48-49 0.499140156122202 1116 50-51 0.38897402689551086 1116 52-53 0.38100600104048965 1116 54-55 0.3854960198529156 1116 56-57 0.35580452894062375 1116 58-59 0.375075328416024 1116 60-61 0.30227333729738604 1116 62-63 0.39229032252072926 1116 64-65 -0.0020875319352171573 1116 66-67 0.24301261790367334 1116 68-69 0.3692055093681432 1116 70-71 0.3986182155031628 1116 72-73 0.40009512691132443 1116 74-75 0.37700111747537335 1116 76 0.6301500608959003 2118 1 0.0865443538569508 2118 2 -0.018960001880621746 2118 3 0.03394135895014472 2118 4 0.05841369184640399 2118 5 0.02814742452694219 2118 6 0.1017175433482933 2118 7 0.10099891791246307 2118 8 0.0471690663167621 2118 9 0.10678210395970922 2118 10-11 0.10571863796246106 2118 12-13 0.0806728441190856 2118 14-15 0.1498782459637482 2118 16-17 0.16907798995240597 2118 18-19 0.1553315779584139 2118 20-21 0.09399143140436905 2118 22-23 0.1303376230833564 2118 24-25 0.07674931714380051 2118 26-27 0.1961528500308063 2118 28-29 0.23643276106130884 2118 30-31 0.18993322768109522 2118 32-33 0.08582211921972771 2118 34-35 0.13833726067456809 2118 36-37 0.23026442591579155 2118 38-39 0.20860134692942012 2118 40-41 1.279646295484632 2118 42-43 0.5674668102465574 2118 44-45 0.2866611529513392 2118 46-47 0.7658584416228678 2118 48-49 0.31481596856696825 2118 50-51 0.1651419310905382 2118 52-53 0.2548464913424695 2118 54-55 0.17110234803656255 2118 56-57 0.1947595882881359 2118 58-59 0.06133876357090884 2118 60-61 0.22727214939686036 2118 62-63 0.23434607940343 2118 64-65 0.19419758636383477 2118 66-67 0.2692752769635618 2118 68-69 0.1959134727364571 2118 70-71 0.26458371728817554 2118 72-73 0.24822295609439493 2118 74-75 0.19768270311666924 2118 76 0.5268750983543669 1119 1 0.07540309275627521 1119 2 0.028480078330723302 1119 3 0.056613797438863855 1119 4 0.030988253389516274 1119 5 0.04707109642431817 1119 6 0.019879684864037017 1119 7 0.0503448134467277 1119 8 0.0954876517959633 1119 9 0.12098778878003458 1119 10-11 0.08786405286522836 1119 12-13 0.17928704980390364 1119 14-15 0.14217779716214096 1119 16-17 0.1725828599272674 1119 18-19 0.18223381241745784 1119 20-21 0.14614114334376893 1119 22-23 0.17291886890568264 1119 24-25 0.06442000498796574 1119 26-27 0.18435867808567963 1119 28-29 0.21927763803874 1119 30-31 0.2719767869006233 1119 32-33 0.24399444630378753 1119 34-35 0.186643114193366 1119 36-37 0.25616043762919105 1119 38-39 0.29524830558238335 1119 40-41 1.3286376854964104 1119 42-43 0.5947418524211727 1119 44-45 0.2671685215734172 1119 46-47 0.7713455186830629 1119 48-49 0.4387086858856222 1119 50-51 0.2628314781510497 1119 52-53 0.31824011581329614 1119 54-55 0.30789970297178826 1119 56-57 0.34079994860261564 1119 58-59 0.29561001798730757 1119 60-61 0.3020787827482323 1119 62-63 0.20106752002474337 1119 64-65 0.3013298714324293 1119 66-67 0.2028422948201296 1119 68-69 0.2651116775300437 1119 70-71 0.3720060523297164 1119 72-73 0.2680032502026819 1119 74-75 0.3013168820643699 1119 76 0.5461274139796615 2119 1 0.018496259077117827 2119 2 0.10088130755391234 2119 3 0.06696270758661171 2119 4 0.030895177142461705 2119 5 0.01870637386375762 2119 6 0.023504546203149346 2119 7 0.05214166378548413 2119 8 0.06114746663347148 2119 9 0.13174227441407282 2119 10-11 0.07685545365868762 2119 12-13 0.10635280285728044 2119 14-15 0.10199226950421547 2119 16-17 0.03201297686900517 2119 18-19 0.08922422807959407 2119 20-21 0.05512105364326203 2119 22-23 0.15431846949741157 2119 24-25 0.11410202828867 2119 26-27 0.1500036429203888 2119 28-29 0.22128453882692867 2119 30-31 0.17184719425324602 2119 32-33 0.20796509360972948 2119 34-35 0.16298499047092463 2119 36-37 0.26439496705045684 2119 38-39 0.1950340760337994 2119 40-41 1.4301794079182102 2119 42-43 0.583019966194918 2119 44-45 0.31673543706554597 2119 46-47 0.8455059863526628 2119 48-49 0.42946362594597787 2119 50-51 0.23192549814418584 2119 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2104 76 -0.7827936588943416 2111 1 -0.33329032671768744 2111 2 -0.14327315217883552 2111 3 -0.1353928330233174 2111 4 -0.042439300976212735 2111 5 -0.08744166749856674 2111 6 -0.08484461383526565 2111 7 -0.035546952565162826 2111 8 -0.14609301111809714 2111 9 -0.2728063478297642 2111 10-11 -0.14365287303199636 2111 12-13 -0.11734499824806477 2111 14-15 -0.06249057117211976 2111 16-17 -0.1400683526524844 2111 18-19 -0.1410765949727093 2111 20-21 -0.058208256714564754 2111 22-23 -0.16845950072181637 2111 24-25 -0.18654138370872886 2111 26-27 -0.09295728741401632 2111 28-29 -0.1153354229522634 2111 30-31 -0.21952368555756152 2111 32-33 -0.3105712178327167 2111 34-35 -0.15816399920509383 2111 36-37 -0.18096712985775554 2111 38-39 -0.17102384355015232 2111 40-41 0.9063900494393025 2111 42-43 -0.5592518116360452 2111 44-45 -0.3673622250237898 2111 46-47 0.10471753915216198 2111 48-49 -0.3881798961073528 2111 50-51 -0.6057360814820427 2111 52-53 -0.6048754084736956 2111 54-55 -0.4213544266714919 2111 56-57 -0.6647087911659071 2111 58-59 -0.622628378467887 2111 60-61 -0.7096634788019784 2111 62-63 -0.8610174979060119 2111 64-65 -0.6268962250623176 2111 66-67 -0.4338593523173131 2111 68-69 -0.46963976981565736 2111 70-71 -0.5092997541389508 2111 72-73 -1.0299525303539383 2111 74-75 -1.3082073747225564 2111 76 -0.5682263472311888 2110 1 -0.13235730439179605 2110 2 -0.05730180929313633 2110 3 -0.09807193998766195 2110 4 -0.061766192012534304 2110 5 -0.07444599938793317 2110 6 0.012123063605585571 2110 7 0.005106163062961855 2110 8 -0.08278078186118165 2110 9 -0.21149345212833737 2110 10-11 -0.21929432588104447 2110 12-13 -0.26529568135369885 2110 14-15 -0.14862852518744774 2110 16-17 -0.20821230466848561 2110 18-19 -0.2318796606174942 2110 20-21 -0.4790679701434257 2110 22-23 -0.2301056186825008 2110 24-25 -0.26968033739083097 2110 26-27 -0.10628617778389327 2110 28-29 -0.3327629471108722 2110 30-31 -0.2425160214456028 2110 32-33 -0.20960487328089528 2110 34-35 -0.09951688157764238 2110 36-37 -0.219454300800777 2110 38-39 -0.1736896216241277 2110 40-41 0.7121214722983567 2110 42-43 -0.3294950638852896 2110 44-45 -0.5164791860367899 2110 46-47 -0.281986892703884 2110 48-49 -0.5312988564539083 2110 50-51 -0.7200313164037411 2110 52-53 -0.7258350885803253 2110 54-55 -0.7585753530293715 2110 56-57 -0.729520520589432 2110 58-59 -0.8273934567751127 2110 60-61 -0.5517161279189438 2110 62-63 -0.7318938724478272 2110 64-65 -0.624896891506836 2110 66-67 -1.1509536542166856 2110 68-69 -1.19639751723318 2110 70-71 -0.7495530030559792 2110 72-73 -1.1384163757388137 2110 74-75 -0.6809164716902387 2110 76 -1.0903856274711075 2109 1 -0.09314756605328256 2109 2 -0.14896640210818646 2109 3 -0.22112035188599322 2109 4 -0.19293707223015133 2109 5 -0.08475456507466816 2109 6 -0.07579711396297739 2109 7 -0.25382100654904605 2109 8 -0.2884584720450931 2109 9 -0.20954953844930202 2109 10-11 -0.32405288971654755 2109 12-13 -0.3393877209973013 2109 14-15 -0.2684378620657739 2109 16-17 -0.5059162814488403 2109 18-19 -0.4469790227147925 2109 20-21 -0.5644080771213638 2109 22-23 -0.5504142851326392 2109 24-25 -0.4702161058243206 2109 26-27 -0.4684220879422938 2109 28-29 -0.5482159770666684 2109 30-31 -0.5457857707228015 2109 32-33 -0.7862314056463475 2109 34-35 -0.36056008283592433 2109 36-37 -0.887824513542796 2109 38-39 -0.5107322787599813 2109 40-41 -10.433319498854722 2109 42-43 -1.273788187462003 2109 44-45 -0.7308873228463213 2109 46-47 -0.18657384238920116 2109 48-49 -0.48970389584302865 2109 50-51 -0.4659131977831734 2109 52-53 -0.35823229466868867 2109 54-55 -0.7431079349957201 2109 56-57 -0.49512449647932044 2109 58-59 -0.543221596106072 2109 60-61 -0.7470214551164887 2109 62-63 -0.49312571218619894 2109 64-65 -0.6554424996046535 2109 66-67 -0.5245014894202313 2109 68-69 -1.0380752913084876 2109 70-71 -0.7890519675361674 2109 72-73 -0.5730346886218598 2109 74-75 -0.6985395823176788 2109 76 -0.5896058826978035 2108 1 -0.014332510346612537 2108 2 -0.1398646557113281 2108 3 -0.1368836740785042 2108 4 0.004284281640856591 2108 5 -0.10484095435625562 2108 6 -0.11073891887893694 2108 7 -0.2749466955922131 2108 8 -0.2667119766846824 2108 9 -0.3208414480599018 2108 10-11 -0.3546647325755643 2108 12-13 -0.42040100132751235 2108 14-15 -0.3538341127585056 2108 16-17 -0.6589261163687965 2108 18-19 -0.6619400791068202 2108 20-21 -0.7599347852718878 2108 22-23 -0.4360821742304495 2108 24-25 -0.9051714803480877 2108 26-27 -0.7874447988259234 2108 28-29 -1.3790147616860153 2108 30-31 -0.7479540048282161 2108 32-33 -1.2717276722545279 2108 34-35 -0.8073359854934878 2108 36-37 -1.4942994715897697 2108 38-39 -0.7649198700988649 2108 40-41 -7.822794460012208 2108 42-43 -0.925819572007839 2108 44-45 -0.49744885421349494 2108 46-47 0.19890754560411494 2108 48-49 -0.1840834731555745 2108 50-51 -0.42439052189281057 2108 52-53 -0.6138016012785741 2108 54-55 -0.6574910971731427 2108 56-57 -0.6591839710205534 2108 58-59 -0.5379244592803616 2108 60-61 -0.8204966044599331 2108 62-63 -0.5970692968333324 2108 64-65 -0.488148027969153 2108 66-67 -0.54664360300265 2108 68-69 -0.4507641965794065 2108 70-71 -0.29955052814682404 2108 72-73 -0.5713089377831864 2108 74-75 -0.6084806096056781 2108 76 -1.0760651767256704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 5.0 15 8.0 16 20.0 17 42.0 18 69.0 19 96.0 20 167.0 21 231.0 22 339.0 23 513.0 24 778.0 25 1061.0 26 1779.0 27 2778.0 28 4337.0 29 6352.0 30 9443.0 31 14009.0 32 20893.0 33 30511.0 34 45543.0 35 73811.0 36 158268.0 37 888459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11827568548211 26.307983628618953 7.684227659059241 23.889513026839694 2 17.179708062230304 24.287046998249327 37.05791514987912 21.47532978964125 3 20.378002643874822 26.11163820994589 30.30364862665391 23.206710519525373 4 14.599349749705242 16.73612461939715 38.99358086247484 29.670944768422764 5 14.356557881406731 33.69495401007531 36.032361663021085 15.916126445496879 6 26.838187715112564 36.272295288265724 20.372365553407462 16.517151443214253 7 23.110959377220595 34.1921295101686 23.07459617392409 19.622314938686717 8 21.052230422027527 39.138716093099326 22.483336839974115 17.32571664489903 9 20.802054759173174 19.62652290762714 23.801939635494616 35.769482697705065 10-11 23.98625661464929 26.23938579532598 28.964522058093788 20.809835531930943 12-13 21.73947908520343 25.140907412773966 29.034985688935823 24.084627813086783 14-15 21.257944526266062 28.823277213848186 24.35743123345097 25.561347026434778 16-17 20.644176528266833 32.17873546563558 26.66820164904745 20.508886357050134 18-19 20.088645232490286 30.824444329761853 29.139351258222412 19.947559179525452 20-21 20.4531109196794 28.960274391333172 29.39008269055946 21.196531998427968 22-23 20.85548802515254 29.250822737323496 28.917559536805832 20.976129700718133 24-25 20.78859719812785 29.897142947880734 28.778259885749673 20.535999968241743 26-27 20.455532486711157 30.053314172518785 28.90926269238556 20.581890648384498 28-29 20.254939401277475 29.76840291699583 29.426961965518473 20.549695716208223 30-31 20.454500343386144 29.390717855682546 29.5121137898318 20.64266801109951 32-33 20.5726410562796 29.698812638198035 29.095048490887365 20.633497814635 34-35 20.529132245348407 29.511121344326984 29.310091582871184 20.649654827453425 36-37 20.363989313346803 29.65347772753798 29.60314089153365 20.37939206758157 38-39 20.30257678550871 29.30898004390579 29.665903145258294 20.72254002532721 40-41 20.308452062897224 29.20810788279616 29.72386196273963 20.75957809156699 42-43 20.553546404766916 29.192586035100813 29.59730531196532 20.656562248166953 44-45 20.011274180934723 28.92958797632422 30.111908155123203 20.947229687617853 46-47 19.998729669753835 28.672901870958267 30.570854654370926 20.75751380491697 48-49 19.749308265483144 28.716926753551963 30.559024703953508 20.97474027701139 50-51 19.542522320099405 28.896519692103706 30.466846365466072 21.094111622330818 52-53 19.502586312985553 28.712520295510576 30.498723715080807 21.286169676423068 54-55 19.271028927801577 28.556746049074444 30.718808430229096 21.453416592894882 56-57 18.923037835992425 28.53812777140407 30.848183626237084 21.690650766366417 58-59 18.838282989881026 28.50228063977007 30.79844225753564 21.860994112813266 60-61 18.90735719701631 28.394699547047868 30.612497667753065 22.085445588182754 62-63 18.630703088093433 28.068701047625478 30.72643041170609 22.574165452575 64-65 18.74265094103683 27.89887377284113 30.336836004334998 23.02163928178704 66-67 18.91144607249616 28.112964117140326 29.790593998483544 23.18499581187997 68-69 18.673140057879422 28.273383008539 29.65331893625721 23.400157997324367 70-71 18.577388915574648 28.22951691722608 29.67233419212951 23.52075997506977 72-73 18.46512348006971 28.344719991425272 29.611278944673145 23.578877583831872 74-75 18.913629452606756 28.3304287761559 29.002354080737426 23.753587690499913 76 19.218667502967413 28.191962779323788 28.419193102106767 24.17017661560204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29.0 1 15.5 2 5.5 3 8.0 4 7.0 5 8.5 6 11.0 7 15.5 8 19.0 9 41.5 10 111.5 11 269.0 12 379.0 13 564.5 14 864.5 15 1274.0 16 2017.0 17 2465.0 18 3329.0 19 5184.0 20 7254.5 21 8334.0 22 9618.0 23 13066.0 24 17110.0 25 18990.0 26 21040.5 27 25223.0 28 29142.5 29 30930.0 30 33155.0 31 37271.5 32 40675.0 33 42187.0 34 43875.0 35 47602.5 36 51451.5 37 53261.0 38 54555.5 39 57138.0 40 59314.5 41 60127.0 42 60051.0 43 60807.0 44 61320.0 45 60402.5 46 59728.0 47 58359.5 48 55599.0 49 52507.0 50 50807.0 51 49377.5 52 45265.5 53 39918.5 54 37254.0 55 34545.0 56 29241.5 57 23917.5 58 21188.0 59 18827.5 60 14455.5 61 10729.5 62 9015.0 63 7700.5 64 5317.5 65 3518.0 66 2310.0 67 1833.0 68 1540.0 69 1008.5 70 626.0 71 482.0 72 376.0 73 224.0 74 133.0 75 88.0 76 64.0 77 32.5 78 18.5 79 12.0 80 9.5 81 6.5 82 3.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1259515.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.00915138671122 #Duplication Level Percentage of deduplicated Percentage of total 1 95.26109832849143 85.74370620713569 2 3.4328902839934594 6.179830825318748 3 0.5473086940685191 1.477883732990297 4 0.18495789049150374 0.6659161106166606 5 0.10362692526784066 0.46636858020862404 6 0.07492418792837947 0.40463175442631455 7 0.08455815678765477 0.5327705554696897 8 0.0618531428765081 0.4453879120732398 9 0.03466904350263946 0.2808478066555397 >10 0.20592109090679594 3.1627991640276716 >50 0.006775064379641746 0.3976245913832035 >100 0.0014171913056201541 0.24223275969426292 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.969782019269322E-4 0.0 0.0 2 4.763738423123186E-4 0.0 0.0 3 4.763738423123186E-4 0.0 0.0 4 4.763738423123186E-4 0.0 7.939564038538644E-5 5 4.763738423123186E-4 0.0 7.939564038538644E-5 6 4.763738423123186E-4 0.0 7.939564038538644E-5 7 4.763738423123186E-4 0.0 7.939564038538644E-5 8 4.763738423123186E-4 0.0 7.939564038538644E-5 9 4.763738423123186E-4 0.0 7.939564038538644E-5 10 4.763738423123186E-4 0.0 1.5879128077077287E-4 11 4.763738423123186E-4 0.0 1.5879128077077287E-4 12 4.763738423123186E-4 0.0 2.381869211561593E-4 13 4.763738423123186E-4 0.0 2.381869211561593E-4 14 4.763738423123186E-4 0.0 2.381869211561593E-4 15 4.763738423123186E-4 0.0 2.381869211561593E-4 16 4.763738423123186E-4 7.939564038538644E-5 2.381869211561593E-4 17 4.763738423123186E-4 7.939564038538644E-5 2.381869211561593E-4 18 4.763738423123186E-4 1.5879128077077287E-4 3.1758256154154575E-4 19 5.557694826977051E-4 1.5879128077077287E-4 4.763738423123186E-4 20 5.557694826977051E-4 1.5879128077077287E-4 4.763738423123186E-4 21 6.351651230830915E-4 2.381869211561593E-4 9.527476846246372E-4 22 7.145607634684779E-4 2.381869211561593E-4 0.001270330246166183 23 7.145607634684779E-4 2.381869211561593E-4 0.0016673084480931153 24 7.145607634684779E-4 3.969782019269322E-4 0.0020642866500200476 25 7.145607634684779E-4 3.969782019269322E-4 0.002699451773103139 26 7.939564038538644E-4 3.969782019269322E-4 0.004049177659654709 27 7.939564038538644E-4 3.969782019269322E-4 0.01691127140208731 28 7.939564038538644E-4 3.969782019269322E-4 0.03874507250806858 29 7.939564038538644E-4 3.969782019269322E-4 0.06399288615062147 30 7.939564038538644E-4 3.969782019269322E-4 0.0936074600143706 31 7.939564038538644E-4 3.969782019269322E-4 0.11829950417422579 32 7.939564038538644E-4 3.969782019269322E-4 0.14211819628984174 33 7.939564038538644E-4 3.969782019269322E-4 0.17355886988245475 34 7.939564038538644E-4 3.969782019269322E-4 0.21682949389249037 35 7.939564038538644E-4 3.969782019269322E-4 0.27891688467386255 36 7.939564038538644E-4 3.969782019269322E-4 0.39086473761725743 37 7.939564038538644E-4 3.969782019269322E-4 0.5704576761690016 38 8.733520442392508E-4 3.969782019269322E-4 0.782999805480681 39 8.733520442392508E-4 3.969782019269322E-4 1.0182490879425812 40 8.733520442392508E-4 3.969782019269322E-4 1.3034382282068893 41 8.733520442392508E-4 3.969782019269322E-4 1.6176861728522487 42 8.733520442392508E-4 3.969782019269322E-4 1.962501439045982 43 9.527476846246372E-4 3.969782019269322E-4 2.3225606681937094 44 9.527476846246372E-4 3.969782019269322E-4 2.7437545404381845 45 9.527476846246372E-4 3.969782019269322E-4 3.2298146508775205 46 9.527476846246372E-4 4.763738423123186E-4 3.7399316403536282 47 0.0010321433250100238 4.763738423123186E-4 4.252827477243224 48 0.0010321433250100238 4.763738423123186E-4 4.769454909230934 49 0.0010321433250100238 4.763738423123186E-4 5.267027387526151 50 0.0011115389653954101 4.763738423123186E-4 5.789133118700452 51 0.0011115389653954101 4.763738423123186E-4 6.346808096767407 52 0.0011115389653954101 4.763738423123186E-4 6.9279841843884356 53 0.0011115389653954101 4.763738423123186E-4 7.531470446957758 54 0.0011909346057807967 4.763738423123186E-4 8.17695700329095 55 0.0011909346057807967 4.763738423123186E-4 8.85928313676296 56 0.0011909346057807967 4.763738423123186E-4 9.565666149271744 57 0.0011909346057807967 4.763738423123186E-4 10.244340083286026 58 0.0011909346057807967 4.763738423123186E-4 10.891096969865385 59 0.0011909346057807967 4.763738423123186E-4 11.518243133269552 60 0.0011909346057807967 4.763738423123186E-4 12.136496984950556 61 0.001270330246166183 4.763738423123186E-4 12.751892593577686 62 0.001270330246166183 4.763738423123186E-4 13.428581636582335 63 0.0013497258865515695 4.763738423123186E-4 14.104238536261974 64 0.0015085171673223424 5.557694826977051E-4 14.862228715021258 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCG 155 8.7506123E-7 20.32258 5 CTAAGGC 915 0.0 19.508196 70 GTGTTAG 295 1.8189894E-12 18.983051 1 ACTAAGG 1125 0.0 18.355556 70 GTGTAGC 290 1.8189894E-11 18.103449 1 TAGACGG 155 1.9848483E-5 18.064516 5 GCCCTTA 160 2.6077361E-5 17.5 1 TTAGCCC 145 2.4395547E-4 16.896551 4 TAAGGCG 750 0.0 16.800001 70 GTCCTGG 370 1.8189894E-12 16.081081 1 GTAGCAC 305 7.003109E-10 16.065573 3 TAGGCCG 175 5.621546E-5 16.0 5 GACCCGT 155 4.0555312E-4 15.806452 6 GCACCGC 405 0.0 15.555555 6 GACTAAG 1555 0.0 15.305466 70 GTACTAG 165 6.5207423E-4 14.848486 1 GTCTTAC 245 4.270538E-6 14.285714 1 CTACACC 495 0.0 14.141415 4 CTAGACG 175 0.001018041 14.0 4 CTTACCC 175 0.001018041 14.0 3 >>END_MODULE