FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n01 ve08.3410000000c760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n01 ve08.3410000000c760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259515
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG1558.7506123E-720.322585
CTAAGGC9150.019.50819670
GTGTTAG2951.8189894E-1218.9830511
ACTAAGG11250.018.35555670
GTGTAGC2901.8189894E-1118.1034491
TAGACGG1551.9848483E-518.0645165
GCCCTTA1602.6077361E-517.51
TTAGCCC1452.4395547E-416.8965514
TAAGGCG7500.016.80000170
GTCCTGG3701.8189894E-1216.0810811
GTAGCAC3057.003109E-1016.0655733
TAGGCCG1755.621546E-516.05
GACCCGT1554.0555312E-415.8064526
GCACCGC4050.015.5555556
GACTAAG15550.015.30546670
GTACTAG1656.5207423E-414.8484861
GTCTTAC2454.270538E-614.2857141
CTACACC4950.014.1414154
CTAGACG1750.00101804114.04
CTTACCC1750.00101804114.03