##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFJV4 l01n03.3330000000da8f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9761139
Sequences flagged as poor quality	0
Sequence length	301
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.27811006481928	23.0	12.0	32.0	12.0	34.0
2	25.806850614462103	31.0	21.0	34.0	11.0	34.0
3	15.182996574477631	11.0	2.0	31.0	2.0	34.0
4	18.385332592845977	23.0	2.0	31.0	2.0	34.0
5	20.452218434754386	23.0	11.0	32.0	2.0	34.0
6	17.456459333280677	12.0	2.0	34.0	2.0	37.0
7	2.0	2.0	2.0	2.0	2.0	2.0
8	14.086895392023411	12.0	2.0	25.0	2.0	28.0
9	23.690806984717664	25.0	21.0	28.0	11.0	32.0
10-14	20.62204947598841	19.0	13.6	29.0	7.4	34.0
15-19	13.43991298556449	11.2	5.4	20.4	3.8	28.2
20-24	17.325246654104607	20.2	11.0	22.8	7.4	24.0
25-29	10.471231687203716	8.4	4.0	15.8	2.0	22.2
30-34	22.995779693332917	25.8	11.4	30.4	9.2	33.6
35-39	17.000089046985188	13.2	7.6	26.2	3.8	33.4
40-44	21.46396735053153	24.2	11.0	29.6	8.8	33.4
45-49	20.344302770404152	20.4	9.0	30.2	7.4	35.4
50-54	9.730647396784331	10.2	5.6	11.2	5.6	13.0
55-59	16.88920749924778	16.4	9.2	22.0	5.6	30.2
60-64	9.139495462568457	5.8	3.8	12.8	3.8	20.6
65-69	18.144031634013203	18.2	10.0	26.4	7.4	31.6
70-74	22.85306136917013	23.8	11.0	32.2	11.0	36.4
75-79	23.102223090973297	25.2	13.2	33.8	7.4	36.8
80-84	23.915169346528106	25.8	13.0	33.2	11.0	37.0
85-89	9.275822073633005	6.6	3.8	12.6	3.8	17.8
90-94	20.915204731742882	23.2	11.0	28.2	10.8	31.0
95-99	22.92388505070976	23.6	10.8	33.8	9.2	36.8
100-104	23.55370398884802	24.6	12.4	32.2	10.6	36.4
105-109	24.204560389929902	25.2	12.6	33.4	11.0	36.8
110-114	23.8251088320738	25.8	12.6	34.2	11.0	37.0
115-119	20.581308636215507	19.6	11.0	30.0	10.2	37.0
120-124	23.139741847749534	24.2	11.0	32.8	11.0	37.0
125-129	24.564141110991248	26.8	11.0	34.0	10.0	37.0
130-134	22.151634640178777	23.2	10.6	32.8	6.8	36.8
135-139	21.1951253844454	21.2	10.8	31.2	10.2	36.6
140-144	20.69176170936609	19.8	11.0	30.0	10.0	36.2
145-149	20.41759831511466	19.2	10.8	29.2	10.4	35.4
150-154	19.93005877695216	18.6	10.6	28.6	10.0	35.4
155-159	19.833077225926193	18.6	10.8	28.6	10.6	35.2
160-164	19.19108731060996	16.2	10.6	27.4	10.2	35.0
165-169	19.54593505942288	18.2	11.0	28.2	10.4	35.2
170-174	18.827532442679075	17.4	10.8	26.8	9.8	34.2
175-179	20.153083630916434	19.6	11.0	29.2	10.2	35.2
180-184	20.559093544308716	20.0	11.0	30.0	10.0	35.6
185-189	18.48732175620079	15.2	11.0	25.4	10.0	33.6
190-194	17.428630080977232	13.0	10.8	22.2	9.6	32.8
195-199	16.552582214022358	12.0	10.0	21.4	8.8	30.6
200-204	17.864691733208595	16.0	10.4	24.8	9.6	32.2
205-209	18.257280466961898	15.0	10.8	25.2	10.4	32.8
210-214	16.14793980497563	11.8	10.0	20.4	9.0	29.2
215-219	16.42307456127815	11.0	10.6	20.6	9.4	30.0
220-224	15.657320790125004	10.8	9.4	19.4	8.8	28.6
225-229	15.195219779167166	11.2	9.4	19.2	8.4	27.6
230-234	15.732607700802129	11.6	9.8	20.2	9.0	28.6
235-239	16.283409938122997	11.6	9.8	20.8	9.0	29.4
240-244	15.53819092218644	11.6	9.6	20.0	8.8	28.2
245-249	12.710184989682043	9.6	8.4	15.6	7.6	22.8
250-254	13.399260864946191	10.2	9.0	16.8	8.0	23.8
255-259	12.917482949479563	10.0	8.6	16.2	7.6	23.2
260-264	12.612506983047776	10.0	8.4	15.8	7.4	22.2
265-269	12.824448950066175	9.8	8.6	16.2	7.8	23.0
270-274	12.803172519108681	10.0	8.8	16.4	7.6	22.8
275-279	13.426057737729172	10.0	9.0	16.8	7.4	24.4
280-284	13.896507405539456	10.2	9.0	17.0	8.8	25.8
285-289	13.805825754555897	10.8	9.0	17.0	7.6	25.6
290-294	13.434586066236738	10.2	9.0	17.0	8.6	25.2
295-299	13.195884599123115	10.2	9.0	17.0	7.4	25.0
300-301	13.157939508903624	11.0	9.0	17.0	9.0	23.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2103	1	2.0133337594462795
2103	2	-0.8507927215984239
2103	3	9.367232560041318
2103	4	8.265713461788316
2103	5	4.391573908540501
2103	6	11.01862073102058
2103	7	0.0
2103	8	7.998986217239768
2103	9	0.7642748978437517
2103	10-14	6.192691328385621
2103	15-19	3.9466207424663047
2103	20-24	2.518877254184826
2103	25-29	4.8625782519311915
2103	30-34	2.703775861535309
2103	35-39	6.170372604379473
2103	40-44	2.382154362171377
2103	45-49	6.587392001601746
2103	50-54	3.004095028589237
2103	55-59	-4.752693428390868
2103	60-64	-0.6712749858859013
2103	65-69	5.44066455764214
2103	70-74	2.107138025681028
2103	75-79	4.219048635105953
2103	80-84	1.5620293386070685
2103	85-89	4.249219983912219
2103	90-94	3.107630914935026
2103	95-99	3.3497174890228365
2103	100-104	1.800340387082347
2103	105-109	1.6330017416071136
2103	110-114	1.0951785411235448
2103	115-119	0.6572217563968721
2103	120-124	0.3493025935976881
2103	125-129	0.5228198733954876
2103	130-134	1.3337417994702498
2103	135-139	0.2689358657421437
2103	140-144	0.30235642511757277
2103	145-149	0.17923341181507624
2103	150-154	0.05386494101257
2103	155-159	1.0966209713350032
2103	160-164	-0.4608392046255254
2103	165-169	-0.8216139215705383
2103	170-174	-0.4217708263406621
2103	175-179	-0.3598839654048156
2103	180-184	-0.2935695050402458
2103	185-189	0.1168756442123211
2103	190-194	-0.5577503047442498
2103	195-199	-0.28071038746177024
2103	200-204	0.17909323883779749
2103	205-209	0.649231165806814
2103	210-214	0.4026612756858583
2103	215-219	-0.17647787647293356
2103	220-224	-0.578017117072152
2103	225-229	-0.3525589625075334
2103	230-234	0.12170034820663567
2103	235-239	0.2365531443115465
2103	240-244	0.10699380292223815
2103	245-249	-0.5806203531923746
2103	250-254	0.13562164351630024
2103	255-259	-0.7206691599213695
2103	260-264	0.31323141329487214
2103	265-269	-0.17506520726433017
2103	270-274	-0.2675123164170916
2103	275-279	-0.24381199776975215
2103	280-284	-0.6002286669494321
2103	285-289	-0.3592945192409527
2103	290-294	0.4489633894497391
2103	295-299	-1.044405717269255
2103	300-301	-0.1397887447983397
2102	1	-21.118137364383063
2102	2	-5.0044762495026305
2102	3	-13.224598865557018
2102	4	-16.298753889928644
2102	5	-18.50030304255211
2102	6	3.9565608031856847
2102	7	0.0
2102	8	6.967220873081789
2102	9	0.46805159903433946
2102	10-14	-4.3689797900469785
2102	15-19	-5.700374300705043
2102	20-24	-6.695364348934806
2102	25-29	-0.9170301834668741
2102	30-34	-3.2185027968590205
2102	35-39	-10.627828229871248
2102	40-44	0.8429046705308494
2102	45-49	-0.24362685390728345
2102	50-54	-0.6451822681441399
2102	55-59	-3.3148984504128336
2102	60-64	-0.22903904281726284
2102	65-69	-4.764343180765568
2102	70-74	-1.3303225641377274
2102	75-79	-2.861893588513933
2102	80-84	-2.6648534864033344
2102	85-89	-2.578657878115842
2102	90-94	0.12817767410836112
2102	95-99	1.4958838029794315
2102	100-104	-1.9075543010324445
2102	105-109	-0.31122248712650347
2102	110-114	0.13724469823805308
2102	115-119	0.0706436810983746
2102	120-124	-0.49608396287218426
2102	125-129	-0.006626636607798275
2102	130-134	0.9798707994420361
2102	135-139	-0.05112610959593056
2102	140-144	-0.17854891788558902
2102	145-149	0.10125234938458405
2102	150-154	0.07908483538163225
2102	155-159	0.26820008200729717
2102	160-164	0.26281691499593407
2102	165-169	-0.32450280022661815
2102	170-174	-0.2664771362814946
2102	175-179	-0.10122851423411916
2102	180-184	-0.055629417412905724
2102	185-189	0.0694307564610952
2102	190-194	0.6208172319447058
2102	195-199	-0.9576896555242751
2102	200-204	-0.984640104096119
2102	205-209	-0.35117745553864665
2102	210-214	-0.5373525638209795
2102	215-219	-0.1049434062594159
2102	220-224	-0.24055255906287876
2102	225-229	-0.568093390138916
2102	230-234	0.122552060115245
2102	235-239	-0.44338186230696763
2102	240-244	-0.5361395991273348
2102	245-249	-0.07321500112181845
2102	250-254	-0.008710468335641153
2102	255-259	0.6956695114863152
2102	260-264	0.11393337347984556
2102	265-269	-0.2552258772251097
2102	270-274	0.2297455983990897
2102	275-279	0.7377789794284855
2102	280-284	-0.9832931457705101
2102	285-289	0.27857748087028433
2102	290-294	-0.8012498331786979
2102	295-299	0.47259802852896193
2102	300-301	0.2728805631742013
2101	1	1.346192818711792
2101	2	1.4721397771551459
2101	3	-13.224598865557018
2101	4	2.5108029674533228
2101	5	2.29688419592026
2101	6	9.377351280576168
2101	7	0.0
2101	8	-12.380566332187911
2101	9	-0.41929809998447354
2101	10-14	0.7308880020494364
2101	15-19	4.948878367813023
2101	20-24	1.3680316721951122
2101	25-29	4.326258059604568
2101	30-34	-2.6115817103528016
2101	35-39	0.08118624528404084
2101	40-44	-0.3787402713191419
2101	45-49	5.334896061602276
2101	50-54	-0.6308626561065527
2101	55-59	8.36364779403332
2101	60-64	5.863770601324674
2101	65-69	5.70123255274375
2101	70-74	1.1373356092626565
2101	75-79	3.2874364242204592
2101	80-84	-0.6181062704074023
2101	85-89	1.1095785233350686
2101	90-94	0.4848349487234529
2101	95-99	1.5603565213895507
2101	100-104	0.753475649856302
2101	105-109	0.3477413607026669
2101	110-114	0.20325381021244482
2101	115-119	-0.019898016191483237
2101	120-124	0.022460662369308437
2101	125-129	0.2389384381763655
2101	130-134	1.346114531939893
2101	135-139	0.12586463132473824
2101	140-144	-0.08912607121088456
2101	145-149	0.07040113470970866
2101	150-154	-0.19014847036259397
2101	155-159	0.05059375235780905
2101	160-164	0.22967857302974437
2101	165-169	-0.0490052261590499
2101	170-174	0.4152634651119449
2101	175-179	-0.0790004501672179
2101	180-184	0.012806136015402814
2101	185-189	0.1804526724772586
2101	190-194	-4.631478052520597
2101	195-199	-2.539729105934155
2101	200-204	-0.595262091013609
2101	205-209	-1.3709907471388902
2101	210-214	-0.8041376553453716
2101	215-219	0.292835662895115
2101	220-224	-0.10582250184933706
2101	225-229	-0.14118559274177223
2101	230-234	0.42734648860764857
2101	235-239	0.14674086426397892
2101	240-244	0.29445243853224135
2101	245-249	0.41732775391710497
2101	250-254	0.5217485581579293
2101	255-259	0.03838780015471599
2101	260-264	-0.64343722505863
2101	265-269	0.9497946942155888
2101	270-274	1.067183939020234
2101	275-279	0.8191898285521209
2101	280-284	-0.18034736617599556
2101	285-289	0.33377656211366613
2101	290-294	0.5641588991699162
2101	295-299	0.5690372406007942
2101	300-301	0.24686232428300592
2107	1	0.3702027696578263
2107	2	0.18864101272834688
2107	3	9.689331661515437
2107	4	6.570461104045858
2107	5	1.7963839910003934
2107	6	9.371195596040614
2107	7	0.0
2107	8	6.533089347636185
2107	9	0.763136833018411
2107	10-14	-0.33809908107066633
2107	15-19	-0.7605587279303485
2107	20-24	1.2192746296834542
2107	25-29	-4.4915374110559
2107	30-34	0.2500845984195941
2107	35-39	-5.13869579122793
2107	40-44	0.8290611416594658
2107	45-49	-0.45978466411217056
2107	50-54	-0.7282093946062052
2107	55-59	-2.141121480606081
2107	60-64	0.11038125950301492
2107	65-69	0.49571869876055175
2107	70-74	0.7302390369370677
2107	75-79	2.859275961987219
2107	80-84	1.4360012623875456
2107	85-89	0.670920218830938
2107	90-94	1.41378729687856
2107	95-99	1.2059884286491993
2107	100-104	1.005680725094411
2107	105-109	-6.986980423833131
2107	110-114	-0.38764539586902913
2107	115-119	-0.22659996439638164
2107	120-124	0.07841650572057901
2107	125-129	0.20601566769578383
2107	130-134	1.5296361020471743
2107	135-139	0.028950285996391045
2107	140-144	-0.35037792412684254
2107	145-149	-0.029739821328846006
2107	150-154	0.007697347831861379
2107	155-159	-0.10270529155488362
2107	160-164	0.0954122055808746
2107	165-169	0.17349971550434873
2107	170-174	0.32268384449120546
2107	175-179	0.21720711796788095
2107	180-184	0.007647768051107562
2107	185-189	0.16375346111886557
2107	190-194	0.38217508128435185
2107	195-199	-0.35581358181797995
2107	200-204	-0.15560771271767848
2107	205-209	0.16094955155046975
2107	210-214	-0.23640588843845478
2107	215-219	0.25954725882823126
2107	220-224	-0.1548457950780051
2107	225-229	0.03318028623888303
2107	230-234	-0.06785686470597696
2107	235-239	-0.20126551572330698
2107	240-244	0.18438583171183254
2107	245-249	0.16500056349282843
2107	250-254	0.4345334698300629
2107	255-259	0.8778412786942926
2107	260-264	-0.11058777032310907
2107	265-269	0.1262241688170178
2107	270-274	0.49067471049963096
2107	275-279	-0.9788879910786648
2107	280-284	0.14230690800964751
2107	285-289	0.11647367454193258
2107	290-294	-3.8586809455333313
2107	295-299	-0.13653703007967444
2107	300-301	0.17735147055876865
2106	1	0.5411936582920092
2106	2	-0.44144101705921557
2106	3	10.981522986867086
2106	4	-16.298753889928644
2106	5	-1.3712680903660335
2106	6	-15.887921932871201
2106	7	0.0
2106	8	-12.380566332187911
2106	9	-0.6936904228749086
2106	10-14	4.088134476646779
2106	15-19	-6.961383346308564
2106	20-24	0.338345763776573
2106	25-29	5.9881761409207375
2106	30-34	0.8010884502919495
2106	35-39	10.431101193788045
2106	40-44	1.5312495438892242
2106	45-49	-1.688147352029393
2106	50-54	-0.6251991927383695
2106	55-59	2.1114135694942924
2106	60-64	5.614486620091043
2106	65-69	-0.39291653592743714
2106	70-74	0.8128283459618864
2106	75-79	3.1373174485641933
2106	80-84	0.9760493777439656
2106	85-89	-2.6127797504397883
2106	90-94	0.17002876468164985
2106	95-99	1.19792442904631
2106	100-104	0.43727769575897923
2106	105-109	0.5086545806278728
2106	110-114	0.08678099314026255
2106	115-119	0.007410072778167631
2106	120-124	0.155042392697478
2106	125-129	0.24705456742823273
2106	130-134	1.6987203413351857
2106	135-139	-0.18102316500655746
2106	140-144	-0.2464653589366783
2106	145-149	-0.14268227770383746
2106	150-154	-0.40259557254044864
2106	155-159	0.07064387645234049
2106	160-164	0.07304763969426631
2106	165-169	0.27059283983701476
2106	170-174	0.5413555728100903
2106	175-179	0.2758205905111737
2106	180-184	0.04601882943729052
2106	185-189	0.10765216831708813
2106	190-194	0.5104773278981121
2106	195-199	0.012575413763677545
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2115	4	2.800525080583512
2115	5	2.9880246863666677
2115	6	-15.887921932871201
2115	7	0.0
2115	8	8.077061060465994
2115	9	1.0390283633798987
2115	10-14	-4.455739317219788
2115	15-19	10.458719027335114
2115	20-24	2.809700633771964
2115	25-29	4.0531820049061125
2115	30-34	3.3977641142014967
2115	35-39	1.2084528870459934
2115	40-44	0.44198746182668813
2115	45-49	-0.1284895087020672
2115	50-54	3.664993162713513
2115	55-59	3.2931824483391097
2115	60-64	-3.556337682946592
2115	65-69	-0.027134766678909017
2115	70-74	-0.8455557757862344
2115	75-79	-6.004998534231795
2115	80-84	-1.7033984980844288
2115	85-89	-2.4236175517450986
2115	90-94	0.9180540813184415
2115	95-99	-3.6455499270778233
2115	100-104	-0.28744202676003994
2115	105-109	1.0336668710635735
2115	110-114	0.012756820681772751
2115	115-119	0.23985134945527165
2115	120-124	0.32200790479544494
2115	125-129	0.08803913932977636
2115	130-134	1.3273407285798093
2115	135-139	0.3006486353690825
2115	140-144	0.4475542071636731
2115	145-149	0.15249917007581715
2115	150-154	1.0359220453448508
2115	155-159	0.6017487347604309
2115	160-164	-0.4632981970259884
2115	165-169	-0.5019311536177966
2115	170-174	-0.55777787039397
2115	175-179	-0.523293684625127
2115	180-184	-0.3039771121413999
2115	185-189	-0.3245555601074166
2115	190-194	0.49046880426823236
2115	195-199	0.5438421902279842
2115	200-204	-0.06905950981060727
2115	205-209	0.5392904902597522
2115	210-214	0.16667408237648473
2115	215-219	-0.09886207665348579
2115	220-224	0.21638794401969186
2115	225-229	-0.1960867939907338
2115	230-234	0.3774515747134455
2115	235-239	0.06990341021731084
2115	240-244	0.43054440590036336
2115	245-249	0.2968189802425236
2115	250-254	0.32211343600752507
2115	255-259	1.1264004412953526
2115	260-264	0.7601111404635557
2115	265-269	0.5571156445869434
2115	270-274	0.24902464358210885
2115	275-279	0.6154548392275085
2115	280-284	0.39031720428424244
2115	285-289	0.3971022231779475
2115	290-294	0.5622327470877391
2115	295-299	0.8733807949106236
2115	300-301	0.5956934809128285
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	228.0
5	4204.0
6	16878.0
7	31685.0
8	41262.0
9	44423.0
10	43717.0
11	61784.0
12	107073.0
13	235382.0
14	706955.0
15	1652900.0
16	2171241.0
17	1687625.0
18	907547.0
19	430581.0
20	248203.0
21	187755.0
22	167093.0
23	162030.0
24	167544.0
25	175342.0
26	170432.0
27	144926.0
28	106847.0
29	60153.0
30	22340.0
31	4434.0
32	458.0
33	80.0
34	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.619726278202023	11.685693667948845	70.38224670650547	10.312333347343667
2	80.85395370908597	8.25938532986625	6.465680538478198	4.420980422569581
3	8.318289943677904	72.26518265732462	4.38233658802275	15.034190810974735
4	5.446800700948517	76.47266108397334	4.70920200463193	13.371336210446213
5	18.40369396934971	66.47270366489158	3.9403996714108955	11.18320269434781
6	5.891276294523723	79.6148188982297	5.133392635654058	9.360512171592518
7	NaN	NaN	NaN	NaN
8	8.830922079286434	68.13262099821372	4.011172177973674	19.025284744526168
9	7.731126459729751	8.542537914888825	78.69464823726001	5.031687388121407
10-14	25.3922216975943	11.402282708476438	44.12166600325292	19.08382959067634
15-19	37.49160338141977	28.67242536838433	11.087813149154636	22.748158101041263
20-24	29.303930920539916	6.640630942361046	54.02294751498904	10.032490622109998
25-29	38.81811467575234	22.3422584666085	26.124240910644335	12.715385946994818
30-34	29.405424482371945	17.788908107220884	43.76720130337071	9.03846610703646
35-39	31.42601457180934	14.475311549910039	36.30155380217921	17.797120076101415
40-44	13.136246132324795	24.793706743306267	42.900375949113474	19.169671175255466
45-49	11.989973050972923	46.159276963950205	27.038662661607997	14.812087323468875
50-54	34.72180618690533	32.25587365368735	19.82527976050677	13.197040398900548
55-59	6.570331098027352	23.61333630011042	62.46783708512328	7.348495516738948
60-64	4.854032182994339	28.480243849773384	39.80573476095881	26.859989206273465
65-69	17.982828064864634	20.486561519877892	28.165326827249544	33.365283588007934
70-74	15.05813266612894	32.24386653338643	30.144917879431212	22.55308292105342
75-79	10.832572804477678	42.537982494607554	23.304521595578404	23.324923105336364
80-84	22.046307352111707	31.4909008798492	30.912448366617074	15.55034340142202
85-89	4.539098648763167	17.22703913767143	6.334787209248987	71.89907500431642
90-94	19.319191339282987	36.28841155520085	21.459926765287406	22.93247034022875
95-99	4.103762986862037	21.906156169955572	37.55548467525952	36.43459616792287
100-104	4.049775976574503	39.88561874752408	35.328671356807526	20.7359339190939
105-109	4.269592544527584	20.864201698757732	46.00660503738524	28.859600719329443
110-114	4.900889741815846	34.3539590706796	23.17787579002469	37.56727539747987
115-119	15.258569857726373	30.320433702158223	22.834012441105784	31.58698399900962
120-124	6.930512236202614	52.38484147047272	11.335557966800227	29.349088326524438
125-129	3.6987164893645903	46.51084366193551	8.572304358137222	41.21813549056267
130-134	6.022992124226286	49.58836545957127	6.965402380922775	37.42324003527966
135-139	9.022351218757034	36.10276544055467	18.935615726388857	35.93926761429944
140-144	9.924650202885369	32.39641368298956	17.945510444126697	39.733425669998375
145-149	5.348676620077068	26.728865175357292	36.26960922025795	31.652848984307692
150-154	3.7536600343500703	28.211539062281894	43.15165268589674	24.883148217471295
155-159	4.0023758723158895	29.68543292506562	40.85132998737207	25.46086121524642
160-164	7.615562573687611	28.17265340345174	30.275314816788452	33.93646920607219
165-169	6.960195432918097	44.15706900818953	16.458068321182566	32.42466723770981
170-174	5.874666948494985	42.15971399319308	22.737522799149108	29.22809625916283
175-179	8.062014028858467	50.56905626944995	10.299691270976554	31.069238430715025
180-184	6.380083687122013	45.966464460833066	13.34485614828347	34.30859570376145
185-189	11.57805924499202	27.810741051331416	22.833833718536937	37.777365985139625
190-194	6.657401116380483	33.232026194465746	29.44683425116198	30.66373843799179
195-199	6.097170732132824	33.41506349343709	30.404797985987912	30.082967788442172
200-204	4.139993761511677	23.862875245797216	46.58619404597179	25.410936946719314
205-209	5.400491450420775	26.924073162999	44.560787722545356	23.11464766403487
210-214	7.062427375937879	37.594627701805614	28.600464739267395	26.742480182989116
215-219	10.060503928989666	37.124896600686846	29.7537275601093	23.060871910214182
220-224	8.701497378100893	35.73221101717883	23.932226673457947	31.63406493126233
225-229	10.60552050414927	36.194150023771165	18.95334677464036	34.24698269743921
230-234	9.684649500115793	31.195005195253326	19.679788697826574	39.44055660680431
235-239	7.391497655935557	35.343612849052455	17.30078627256765	39.96410322244434
240-244	7.216848465578321	36.9565958041888	22.566852472931487	33.259703257301396
245-249	10.509273918850646	31.23431187404597	29.210551668694574	29.045862538408805
250-254	8.541543894723867	29.25342111208443	34.22839258465752	27.97664240853419
255-259	10.661002922628207	34.073847329874525	27.483406753054545	27.781742994442727
260-264	10.34123754032214	34.255065504743264	24.12671632007014	31.276980634864454
265-269	10.63754339988441	33.06102776519737	25.268363344379413	31.033065490538803
270-274	11.584981526531758	34.15295405521847	21.933962682896947	32.32810173535282
275-279	9.62488719991215	33.876938282737335	20.860211401819676	35.63796311553084
280-284	8.0891056185236	35.246299684607195	17.724789207390753	38.93980548947845
285-289	9.002217783182614	39.59024488867238	12.175985169305552	39.231552158839456
290-294	8.723637250336111	39.974136302963764	13.883311947608213	37.41891449909191
295-299	9.562108972691865	37.413091378655054	18.1945846074843	34.83021504116878
300-301	9.07183824617399	35.42056759336543	18.985452811840258	36.522141348620316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	19.5
2	10.0
3	6.25
4	16.25
5	84.75
6	307.75
7	749.5
8	1462.5
9	2438.25
10	3419.25
11	4083.25
12	4177.75
13	4089.0
14	4286.0
15	4910.0
16	5965.25
17	7223.5
18	8527.75
19	9869.5
20	11244.25
21	13230.5
22	16470.75
23	21591.25
24	29567.25
25	41135.0
26	56526.5
27	75232.0
28	97292.75
29	120759.5
30	144794.25
31	167328.25
32	185218.75
33	197102.0
34	201074.75
35	198279.75
36	190252.5
37	180146.25
38	169612.0
39	158887.75
40	148701.0
41	137283.75
42	123060.5
43	106252.0
44	89622.0
45	73717.0
46	58632.0
47	45644.25
48	34738.75
49	25938.75
50	18768.75
51	13395.0
52	9291.5
53	6546.0
54	4653.75
55	3458.0
56	2632.25
57	2084.75
58	1644.25
59	1295.75
60	1041.0
61	873.5
62	727.5
63	634.75
64	584.0
65	508.25
66	454.5
67	407.75
68	361.5
69	311.5
70	266.5
71	219.0
72	188.25
73	144.5
74	118.0
75	108.0
76	95.0
77	77.25
78	83.0
79	73.0
80	67.75
81	72.0
82	68.5
83	69.0
84	71.75
85	67.75
86	66.0
87	92.5
88	77.25
89	105.5
90	97.75
91	77.25
92	40.5
93	47.25
94	9.25
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	4.697013330104202
2	1.0244706073748155E-5
3	42.19923515073394
4	25.981312221862634
5	10.968494557858463
6	38.672372148373256
7	100.0
8	38.46671991864884
9	0.0
10-14	19.277383510264528
15-19	54.86853737048515
20-24	26.056016618552402
25-29	58.69197232003356
30-34	6.076712973762591
35-39	36.93363038883064
40-44	3.9630457060390185
45-49	17.4828511303855
50-54	56.476007564281176
55-59	30.32017472551103
60-64	62.471193167108886
65-69	20.467084835079184
70-74	5.347736570496537E-4
75-79	11.379807213072162
80-84	0.02294199478155162
85-89	65.73954945216947
90-94	3.025821064529457
95-99	5.249069806300269
100-104	0.18766867268256296
105-109	1.2549314173274246
110-114	0.366539191788991
115-119	0.5465796563290412
120-124	0.67245431091597
125-129	0.7565592498990128
130-134	4.923118091034253
135-139	0.9464735621529413
140-144	0.9620455153850386
145-149	1.0948189550420295
150-154	1.2871653605178657
155-159	1.4627083990915404
160-164	1.567552721050279
165-169	1.708485044624403
170-174	1.746007305090113
175-179	1.7941287384597229
180-184	1.891783325695905
185-189	2.0015614980997607
190-194	3.1456410978268003
195-199	2.2846063353877044
200-204	2.4480790612652887
205-209	2.6006432241155464
210-214	2.663455565994911
215-219	2.68703068361182
220-224	2.9204460667960985
225-229	2.9235727510898064
230-234	4.031799977441157
235-239	3.2002412833174487
240-244	3.4163000854715824
245-249	4.466009550729684
250-254	4.799673480728018
255-259	4.020516458171531
260-264	4.265227654272723
265-269	4.319737686349923
270-274	5.3704408880971775
275-279	6.128866723442828
280-284	5.159272908622651
285-289	6.399132314374379
290-294	6.544877600861948
295-299	7.113073587006598
300-301	5.8033749954795235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	9761139.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.44651912397335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.44336493882044	96.90409763263025
2	0.27517222034158456	0.5362915006380485
3	0.10669985815476571	0.31192589304610857
4	0.0480347230625019	0.1872326623809942
5	0.04100651979861411	0.1997971307881621
6	0.019229725259657604	0.11243218741583869
7	0.012825097691490848	0.08748327892224826
8	0.011226582493151914	0.08751931084926338
9	0.004278554580617924	0.03752372256868334
>10	0.03431434082971119	0.6416425074818786
>50	0.002193226596965305	0.14104709990141034
>100	0.0013048974796591175	0.27673370921308027
>500	1.6508722698712366E-4	0.11600565714605013
>1k	1.7361974468213363E-4	0.29616767287138235
>5k	1.0607919104829053E-5	0.06410003414660491
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	4.097882429499262E-6	0.0	0.0
30-34	4.507670672449189E-5	0.0	0.0
35-39	1.1064282559648009E-4	0.0	2.048941214749631E-5
40-44	1.1269176681122972E-4	0.0	2.048941214749631E-5
45-49	1.1269176681122972E-4	0.0	2.048941214749631E-5
50-54	1.1269176681122972E-4	0.0	2.048941214749631E-5
55-59	1.1269176681122972E-4	0.0	2.048941214749631E-5
60-64	1.454748262472238E-4	0.0	2.048941214749631E-5
65-69	6.105844819953901E-4	0.0	1.2293647288497787E-4
70-74	0.001376888496311752	0.0	1.2293647288497787E-4
75-79	0.0017067680318864427	0.0	1.2293647288497787E-4
80-84	0.001710865914315942	0.0	1.2293647288497787E-4
85-89	0.001749795797396185	0.0	1.2293647288497787E-4
90-94	0.0035713045373086073	0.0	1.6596423839472014E-4
95-99	0.0069438617767865005	0.0	1.9464941540121497E-4
100-104	0.012441171055959762	0.0	6.126334232101398E-4
105-109	0.01962475895487197	0.0	0.0011986306106285343
110-114	0.028806064538165067	0.0	0.0012150221403465313
115-119	0.03998303886462431	0.0	0.0012211689639907801
120-124	0.05073178447720087	0.0	0.0013236160247282619
125-129	0.06193539503945185	0.0	0.0014813844982639833
130-134	0.07508754869692973	0.0	0.0014875313219082323
135-139	0.09177207700863597	0.0	0.0015018739104114796
140-144	0.1094052651027713	0.0	0.0015797336765719656
145-149	0.12788261697738348	0.0	0.0016289082657259565
150-154	0.14638045826414314	0.0	0.001637104030584955
155-159	0.16604824498452486	0.0	0.0016452997954439538
160-164	0.18716258420251983	0.0	0.001659642383947201
165-169	0.20756594081899662	0.0	0.0016739849724504486
170-174	0.22849997321009358	0.0	0.0016801317960946978
175-179	0.24843822017082226	0.0	0.0016801317960946978
180-184	0.2681039579499892	0.0	0.003614332302818349
185-189	0.2878905832608264	8.195764858998525E-6	0.006044376583511412
190-194	0.3077489215141799	1.0244706073748155E-5	0.01477696404077434
195-199	0.3279719713037587	1.0244706073748155E-5	0.014846628042075826
200-204	0.34822165732912935	1.0244706073748155E-5	0.014873264277867573
205-209	0.3677992906360621	1.0244706073748155E-5	0.01494702616159856
210-214	0.3884628627868121	1.0244706073748155E-5	0.01889738482363585
215-219	0.40914897329092437	1.0244706073748155E-5	0.020356230968537586
220-224	0.4288536409531716	1.0244706073748155E-5	0.020356230968537586
225-229	0.4477469278943779	1.0244706073748155E-5	0.02049555897114056
230-234	0.4663513141243046	1.2293647288497788E-5	0.02050375473599956
235-239	0.4850417558852508	2.048941214749631E-5	0.020513999442073307
240-244	0.5020233806730956	2.048941214749631E-5	0.020587761325804293
245-249	0.5184067146262337	2.048941214749631E-5	0.020839781095218498
250-254	0.5339848146819751	2.048941214749631E-5	0.020903298272875737
255-259	0.5490998540231832	2.048941214749631E-5	0.020921738743808482
260-264	0.5628011239262138	7.376188373098673E-5	0.020983206980250975
265-269	0.5760434309971407	1.0244706073748156E-4	0.020993451686324723
270-274	0.5876363403901943	1.0244706073748156E-4	0.021024185804545965
275-279	0.5976208309296692	1.0244706073748156E-4	0.02106721357005571
280-284	0.6060788602641557	1.0244706073748156E-4	0.021081556158558955
285-289	0.612860855684977	1.0244706073748156E-4	0.021083605099773705
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTTC	47445	0.0	1238.8533	50-54
AATTTCA	51840	0.0	1171.7661	55-59
AATGCCC	14970	0.0	1064.9114	80-84
GAATTTA	15260	0.0	936.0384	50-54
ATCCTTG	49730	0.0	885.2259	8
ATTCTTG	25240	0.0	860.531	8
TCCTTGG	68755	0.0	796.13696	9
ATCTTTG	12910	0.0	777.00183	8
GAATGCC	10285	0.0	729.7463	80-84
TCCTAGG	6740	0.0	720.5265	9
GCATTTC	13120	0.0	684.9336	50-54
TTCTAGG	3945	0.0	680.8112	9
TTCTTGG	36170	0.0	664.5927	9
GAATTTG	1820	0.0	643.24615	50-54
TCTTTGG	16300	0.0	639.92664	9
GGATTTC	1385	0.0	604.4436	50-54
TCATTGG	10335	0.0	600.22314	9
GACTTTC	17255	0.0	599.6352	50-54
TAATGCC	11995	0.0	595.65186	80-84
TCTTAGG	2420	0.0	588.4472	9
>>END_MODULE