FastQCFastQC Report
Thu 2 Jul 2015
000000000-AFJV4 l01n03.3330000000da8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJV4 l01n03.3330000000da8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9761139
Sequences flagged as poor quality0
Sequence length301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTC474450.01238.853350-54
AATTTCA518400.01171.766155-59
AATGCCC149700.01064.911480-84
GAATTTA152600.0936.038450-54
ATCCTTG497300.0885.22598
ATTCTTG252400.0860.5318
TCCTTGG687550.0796.136969
ATCTTTG129100.0777.001838
GAATGCC102850.0729.746380-84
TCCTAGG67400.0720.52659
GCATTTC131200.0684.933650-54
TTCTAGG39450.0680.81129
TTCTTGG361700.0664.59279
GAATTTG18200.0643.2461550-54
TCTTTGG163000.0639.926649
GGATTTC13850.0604.443650-54
TCATTGG103350.0600.223149
GACTTTC172550.0599.635250-54
TAATGCC119950.0595.6518680-84
TCTTAGG24200.0588.44729
Produced by FastQC (version 0.11.2)