##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFJV4 l01n02.3320000000da82.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9761139
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.310269938784806	34.0	23.0	34.0	12.0	34.0
2	28.827088416628428	34.0	23.0	34.0	12.0	34.0
3	28.349357385444463	34.0	23.0	34.0	12.0	34.0
4	28.465655596134837	34.0	23.0	34.0	12.0	34.0
5	29.67270438419123	34.0	31.0	34.0	12.0	34.0
6	31.10796055665225	37.0	26.0	38.0	12.0	38.0
7	30.785965961554282	37.0	26.0	38.0	12.0	38.0
8	31.07687658171859	37.0	26.0	38.0	12.0	38.0
9	28.879985522181375	34.0	21.0	38.0	11.0	38.0
10	30.996985802578983	36.0	26.0	38.0	11.0	38.0
11	30.30308563375647	37.0	24.0	38.0	11.0	38.0
12	30.417017317343806	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2103	1	-1.2001197612321555
2103	2	-0.5355971868953802
2103	3	0.12822226247547164
2103	4	-0.06090480988904545
2103	5	-0.05789410865660116
2103	6	-0.06616758750288199
2103	7	-0.059791836892138406
2103	8	-0.33686887460326176
2103	9	1.4078259535255064
2103	10	0.13126321170202715
2103	11	0.6894158147868019
2103	12	0.06179042791878331
2102	1	0.3863139716416768
2102	2	0.4078388340995076
2102	3	0.7688268909838989
2102	4	0.5284309620577865
2102	5	0.20101284877955194
2102	6	0.661672655906024
2102	7	0.25762827108497177
2102	8	0.29046504444890076
2102	9	2.060270925382767
2102	10	0.6826347555752648
2102	11	0.8072995073107307
2102	12	0.6783662882737254
2101	1	-0.10415136861386642
2101	2	0.38999003718085845
2101	3	0.8325927903736208
2101	4	0.763640969370865
2101	5	0.3259030797660145
2101	6	0.635187976745673
2101	7	0.4477698327840294
2101	8	0.40208493943053725
2101	9	2.2822654114933343
2101	10	0.7084026728383712
2101	11	1.4226429517032209
2101	12	0.8252350039665508
2107	1	0.48766224945498493
2107	2	0.25233217509853034
2107	3	0.8239786772261084
2107	4	0.5284393535058953
2107	5	0.2560066823932914
2107	6	0.5601436449214852
2107	7	0.16675004093606915
2107	8	0.3044158262368484
2107	9	2.282730364122589
2107	10	0.618045887856443
2107	11	1.2479940859118095
2107	12	-0.012788708038403485
2106	1	0.37373548791281763
2106	2	0.5500298267975374
2106	3	0.6829197496682937
2106	4	0.4192046107989924
2106	5	0.21830539655276482
2106	6	0.23489039199741413
2106	7	-0.3605836517119556
2106	8	0.23427021726571695
2106	9	2.1177356668581524
2106	10	0.7697081235101741
2106	11	1.1655520830968875
2106	12	0.42468272718836886
2105	1	0.22016392071943613
2105	2	-0.5323104601157596
2105	3	0.5789711104017421
2105	4	0.6294207416889428
2105	5	0.19723994415383572
2105	6	0.570812067116993
2105	7	0.6032850642455863
2105	8	0.6522655489795923
2105	9	2.5848248511473777
2105	10	0.7311059791335168
2105	11	-12.081623467211116
2105	12	-2.3024941520107625
2104	1	0.3707362425696168
2104	2	0.32538095886130947
2104	3	0.7785437320316362
2104	4	0.6341409642227624
2104	5	0.243849106799658
2104	6	0.3071468558463408
2104	7	0.2483570112664495
2104	8	0.5324311398617567
2104	9	2.43971006932334
2104	10	0.6924867953871967
2104	11	1.0502299619080375
2104	12	0.3406570142949583
2111	1	0.17399263364357154
2111	2	0.2706793612911973
2111	3	0.7636383964173241
2111	4	0.46733661158618034
2111	5	0.238614606600418
2111	6	0.10370541338915729
2111	7	-0.00920846073735504
2111	8	0.4043483986058689
2111	9	2.094595502909545
2111	10	0.5696886151986646
2111	11	1.0057161272156776
2111	12	0.450491569574325
2110	1	-0.2172435870016507
2110	2	-0.298427469887681
2110	3	0.17003386310290125
2110	4	-0.3169786893376454
2110	5	-0.26395041837186284
2110	6	-0.8214993873700038
2110	7	-1.068577836544545
2110	8	-0.7780500558956547
2110	9	-10.53155265881453
2110	10	-2.6578379321689845
2110	11	-0.9996920752722218
2110	12	-0.9166121750230083
2109	1	-0.17594686880102728
2109	2	-0.3967552775195706
2109	3	0.03358454322576421
2109	4	-0.2086643492971092
2109	5	-0.29337784628985375
2109	6	-0.706374161564657
2109	7	-0.6775976656258038
2109	8	-0.7420189569667919
2109	9	-10.573273592846125
2109	10	-2.6819855931959786
2109	11	-1.0158510574338386
2109	12	-1.29389690345824
2108	1	0.31508840297114205
2108	2	0.24774992605734525
2108	3	0.5505587876263007
2108	4	0.45849739136817647
2108	5	0.13520167753588197
2108	6	0.2464751191680854
2108	7	0.2304286168919738
2108	8	0.1758673117543914
2108	9	-10.354083445888747
2108	10	-2.0838827227518593
2108	11	-0.02997363110079121
2108	12	-0.06618280033442758
2116	1	0.4397079070751104
2116	2	0.25824036630433866
2116	3	0.6834852871216626
2116	4	0.5872243416462233
2116	5	0.1680231831487049
2116	6	0.4425641740729134
2116	7	0.575793137159728
2116	8	0.3009753488999074
2116	9	1.7397299637314347
2116	10	0.258072551095335
2116	11	1.063053982831267
2116	12	0.5692082064598871
2117	1	0.38630966501107267
2117	2	0.23666503388365
2117	3	0.589587530772544
2117	4	0.4741364089752693
2117	5	0.10448131223253299
2117	6	0.37132116029525264
2117	7	0.4958921948169248
2117	8	0.0013589510327136622
2117	9	1.9995414538506822
2117	10	0.5850607430502528
2117	11	1.1037572583506936
2117	12	0.48253734169349016
2118	1	0.2929519300497674
2118	2	0.21871254097032633
2118	3	0.628665340635969
2118	4	0.4814072014459825
2118	5	0.19109586301035364
2118	6	0.39693583093137974
2118	7	0.44646425946041646
2118	8	0.19439416745965232
2118	9	2.01133077443345
2118	10	0.5182166195786095
2118	11	0.9090724543048587
2118	12	0.5126325801449099
2119	1	0.5012911671702014
2119	2	0.24155856214319016
2119	3	0.7146001243718239
2119	4	0.4997358592607384
2119	5	0.09753178202835144
2119	6	0.20180056541326508
2119	7	0.30209050863862075
2119	8	-0.08638630173745554
2119	9	2.074357565828649
2119	10	0.6159916846273745
2119	11	0.9631872466530993
2119	12	0.504355409768344
2112	1	-0.8093152794946405
2112	2	-0.8817669793259988
2112	3	-0.11227448421193387
2112	4	-4.0883077379800135
2112	5	-1.0259444842883028
2112	6	-1.2404711057435414
2112	7	-1.047496923546344
2112	8	-1.144534056614809
2112	9	1.008849427459893
2112	10	-0.40971622744023506
2112	11	-0.0835088498573775
2112	12	-0.3250398130686385
2113	1	-1.3101057147827717
2113	2	-0.2847686495718804
2113	3	-7.8873714292037
2113	4	-1.3289925032488341
2113	5	-0.4673504283336918
2113	6	-0.7300113141319251
2113	7	-0.6538030865524078
2113	8	-0.09098029051308743
2113	9	2.021498801938751
2113	10	0.5166785577295165
2113	11	1.2695907187700861
2113	12	0.5068448686117435
2114	1	0.26165280136075353
2114	2	0.2041701676301031
2114	3	-0.7082004806361937
2114	4	0.05529793673870387
2114	5	0.09791550259749471
2114	6	-0.3579403831182226
2114	7	0.2850689998664713
2114	8	0.1375941443932014
2114	9	2.203676109620975
2114	10	0.6995053168526688
2114	11	1.2580668068561494
2114	12	0.15783564141505124
2115	1	-0.39272379965404625
2115	2	-0.6737217670016769
2115	3	-0.02036269238326227
2115	4	-0.523065262913974
2115	5	-0.3666636996586021
2115	6	-0.8101919163727835
2115	7	-0.1824684755407695
2115	8	-0.4516325020380414
2115	9	1.1299668559228309
2115	10	-0.263439038578305
2115	11	0.2550700811759796
2115	12	-0.597622527376533
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	215.0
11	6090.0
12	25428.0
13	65881.0
14	118248.0
15	172993.0
16	220354.0
17	258258.0
18	278738.0
19	287181.0
20	282599.0
21	271860.0
22	258778.0
23	244397.0
24	231158.0
25	221123.0
26	214603.0
27	211866.0
28	216807.0
29	229068.0
30	252966.0
31	296540.0
32	387765.0
33	791649.0
34	680702.0
35	1005951.0
36	2529921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.812309112091796	25.15982028004611	40.68409636589173	13.343774241970364
2	9.077250103702038	26.94283935512034	27.189357717372943	36.79055282380468
3	47.05765382503005	16.045525015062278	16.16188438664791	20.73493677325976
4	15.602144380896533	32.527074965329355	26.917268568760267	24.95351208501385
5	64.59004425610577	9.89518743663009	20.829085622077507	4.685682685186636
6	22.280617046842586	24.17357236691333	25.389455062570054	28.15635552367403
7	19.61692175472555	31.2701109983169	28.591745287102256	20.5212219598553
8	23.23349764817405	23.806084515341908	25.730019826579664	27.230398009904377
9	26.439058642547618	24.91254605764205	28.36394690864938	20.284448391160947
10	18.63674925641362	9.720597155721274	45.39631081987461	26.246342767990498
11	20.719293107085146	17.127335242331863	32.14869699120154	30.004674659381454
12	23.651297251273647	26.906050615609512	21.522928830334248	27.919723302782597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1834.0
1	1834.0
2	1834.0
3	1834.0
4	6956.5
5	12079.0
6	12079.0
7	12079.0
8	12079.0
9	12079.0
10	12079.0
11	12079.0
12	12079.0
13	31546.0
14	51013.0
15	51013.0
16	51013.0
17	51013.0
18	51013.0
19	51013.0
20	51013.0
21	101924.5
22	152836.0
23	152836.0
24	152836.0
25	152836.0
26	152836.0
27	152836.0
28	152836.0
29	297231.0
30	441626.0
31	441626.0
32	441626.0
33	441626.0
34	441626.0
35	441626.0
36	441626.0
37	441626.0
38	1478069.5
39	2514513.0
40	2514513.0
41	2514513.0
42	2514513.0
43	2514513.0
44	2514513.0
45	2514513.0
46	3078023.5
47	3641534.0
48	3641534.0
49	3641534.0
50	3641534.0
51	3641534.0
52	3641534.0
53	3641534.0
54	3094679.0
55	2547824.0
56	2547824.0
57	2547824.0
58	2547824.0
59	2547824.0
60	2547824.0
61	2547824.0
62	2547824.0
63	1445903.0
64	343982.0
65	343982.0
66	343982.0
67	343982.0
68	343982.0
69	343982.0
70	343982.0
71	195087.0
72	46192.0
73	46192.0
74	46192.0
75	46192.0
76	46192.0
77	46192.0
78	46192.0
79	25998.0
80	5804.0
81	5804.0
82	5804.0
83	5804.0
84	5804.0
85	5804.0
86	5804.0
87	5804.0
88	3291.5
89	779.0
90	779.0
91	779.0
92	779.0
93	779.0
94	779.0
95	779.0
96	951.0
97	1123.0
98	1123.0
99	1123.0
100	1123.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0017928235629059272
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0244706073748155E-5
10	0.0
11	0.0
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	9761139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.245085038191159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.32174983580846	3.829595424153086
2	13.354001733773396	1.6679375285513407
3	5.846121496347263	1.0952857766485797
4	3.987504211594101	0.9960921156620222
5	2.3492671483493757	0.7335686559435349
6	1.9782187515621525	0.7412486596589996
7	1.421460275193815	0.6213998207897188
8	1.1867161597030405	0.5928914666832904
9	0.8872338286072436	0.4986765637570941
>10	6.5308141842153775	8.006588886033482
>50	0.6668685094623376	2.87490849130894
>100	0.3830843084184937	4.460285234957576
>500	0.0342482258916707	1.508023913697946
>1k	0.03986304831879124	5.496214169461218
>5k	0.0037406393361737083	1.684493004709534
>10k+	0.009107643418036349	65.19279028798374
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGGTCAATCT	393251	4.028740908207537	No Hit
TCTAGCGTAGTG	376680	3.858975883859456	No Hit
AAGAGATGTCGA	368274	3.7728588846035285	No Hit
ACGTGTACCCAA	354048	3.627117695998387	No Hit
ACGCGCAGATAC	326411	3.343984754238209	No Hit
CTGCTAACGCAA	319204	3.270151157564706	No Hit
TCACGGGAGTTG	318990	3.267958790464924	No Hit
GACTTTCCCTCG	314267	3.219573043678612	No Hit
TCCAAAGTGTTC	314158	3.2184563707165728	No Hit
TACAGATGGCTC	295946	3.0318797837014717	No Hit
TCGAGGACTGCA	287167	2.9419415090800367	No Hit
TTAGGGCTCGTA	286071	2.930713311223209	No Hit
GGATGGTGTTGC	281290	2.8817333714846187	No Hit
ATGTGCACGACT	270086	2.7669516846343445	No Hit
CGGAGCTATGGT	255997	2.622614020761307	No Hit
ATCCCGAATTTG	246415	2.524449247162652	No Hit
TAGCTCGTAACT	233333	2.3904280023058786	No Hit
TAGGATTGCTCG	208011	2.1310115551064275	No Hit
GCGATATATCGC	200301	2.0520248712778293	No Hit
CAGTGCATATGC	196469	2.012767157603226	No Hit
ACGTGTACGCAA	28100	0.2878762406723232	No Hit
GTTGGTCAGTCT	27431	0.28102253230898566	No Hit
GACTTTCCGTCG	25962	0.26597305908664964	No Hit
TCTAGCGTGGTG	21895	0.2243078394847159	No Hit
TTAGGGCTGGTA	20962	0.21474952871790884	No Hit
AAGAGATGGCGA	20709	0.21215761808125055	No Hit
ACGTGTACTCAA	19621	0.20101137787301254	No Hit
GTGGGTCAATCT	18418	0.18868699646629353	No Hit
TACAGATGTCTC	18326	0.1877444835075087	No Hit
ACGCGCAGGTAC	17664	0.1809624880866874	No Hit
GTTGGTCATTCT	17561	0.17990728336109138	No Hit
ATCCCGAAGTTG	17455	0.17882134451727405	No Hit
GACTTTCCTTCG	16709	0.17117879378625792	No Hit
TCGAGCGTAGTG	16202	0.16598472780686763	No Hit
GGATGGTGGTGC	15905	0.16294205010296442	No Hit
TAGCTCGTGACT	15615	0.15997108534157745	No Hit
TCGAGGACGGCA	15335	0.15710256764092798	No Hit
ACGTGTACACAA	14536	0.1489170474880032	No Hit
CGGAGCTAGGGT	14376	0.1472778945162035	No Hit
TCACGGGATTTG	13797	0.1413462096995033	No Hit
TTAGGGCTTGTA	13699	0.140342228504276	No Hit
TCTAGCGTTGTG	13679	0.14013733438280102	No Hit
GACTTTCCATCG	13473	0.1380269249316089	No Hit
ATGTGCACTACT	13423	0.1375146896279215	No Hit
GAGTTTCCCTCG	13156	0.13477935310623074	No Hit
GCGATATAGCGC	12221	0.12520055292727622	No Hit
TCGCGGGAGTTG	12213	0.12511859527868624	No Hit
TCGAAAGTGTTC	12131	0.1242785293806389	No Hit
TTAGGGCTAGTA	12126	0.12422730585027013	No Hit
GGGTGGTGTTGC	12110	0.12406339055309017	No Hit
TCCAAAGTTTTC	12078	0.12373555995873022	No Hit
ACGCGCAGTTAC	11860	0.12150221403465314	No Hit
AAGAGATGACGA	11394	0.11672818100428649	No Hit
TTGGGGCTCGTA	11146	0.11418749389799696	No Hit
TAGAGATGGCTC	10780	0.11043793147500512	No Hit
ATGCCGAATTTG	10206	0.10455747018867367	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGT	55	0.009159064	6.0000515	1
ACACGGG	205	0.0	6.0000515	1
ACGCCCA	55	0.009159064	6.0000515	1
TGAAGGT	80	6.537357E-5	6.0000515	1
GCGCGCC	65	0.0012616832	6.0000515	1
GTGGGAC	55	0.009159064	6.0000515	1
TCGGGGA	115	6.823393E-8	6.0000515	1
AGGCGCA	65	0.0012616832	6.0000515	1
TGACGGT	135	1.373337E-9	6.0000515	1
TCAGGGC	175	0.0	6.0000515	1
CTGCGCT	55	0.009159064	6.0000515	1
CTGCGCA	175	0.0	6.0000515	1
TCCAGGT	80	6.537357E-5	6.0000515	1
TACGATT	195	0.0	6.0000515	1
CCGAGCT	135	1.373337E-9	6.0000515	1
TTTGGGC	175	0.0	6.0000515	1
TTGCGGA	55	0.009159064	6.0000515	1
GGACGGG	90	9.1490165E-6	6.0000515	1
ACTCGCA	555	0.0	6.0000515	1
TTCGGAC	55	0.009159064	6.0000515	1
>>END_MODULE