FastQCFastQC Report
Thu 2 Jul 2015
000000000-AFJV4 l01n02.3320000000da82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJV4 l01n02.3320000000da82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9761139
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGTCAATCT3932514.028740908207537No Hit
TCTAGCGTAGTG3766803.858975883859456No Hit
AAGAGATGTCGA3682743.7728588846035285No Hit
ACGTGTACCCAA3540483.627117695998387No Hit
ACGCGCAGATAC3264113.343984754238209No Hit
CTGCTAACGCAA3192043.270151157564706No Hit
TCACGGGAGTTG3189903.267958790464924No Hit
GACTTTCCCTCG3142673.219573043678612No Hit
TCCAAAGTGTTC3141583.2184563707165728No Hit
TACAGATGGCTC2959463.0318797837014717No Hit
TCGAGGACTGCA2871672.9419415090800367No Hit
TTAGGGCTCGTA2860712.930713311223209No Hit
GGATGGTGTTGC2812902.8817333714846187No Hit
ATGTGCACGACT2700862.7669516846343445No Hit
CGGAGCTATGGT2559972.622614020761307No Hit
ATCCCGAATTTG2464152.524449247162652No Hit
TAGCTCGTAACT2333332.3904280023058786No Hit
TAGGATTGCTCG2080112.1310115551064275No Hit
GCGATATATCGC2003012.0520248712778293No Hit
CAGTGCATATGC1964692.012767157603226No Hit
ACGTGTACGCAA281000.2878762406723232No Hit
GTTGGTCAGTCT274310.28102253230898566No Hit
GACTTTCCGTCG259620.26597305908664964No Hit
TCTAGCGTGGTG218950.2243078394847159No Hit
TTAGGGCTGGTA209620.21474952871790884No Hit
AAGAGATGGCGA207090.21215761808125055No Hit
ACGTGTACTCAA196210.20101137787301254No Hit
GTGGGTCAATCT184180.18868699646629353No Hit
TACAGATGTCTC183260.1877444835075087No Hit
ACGCGCAGGTAC176640.1809624880866874No Hit
GTTGGTCATTCT175610.17990728336109138No Hit
ATCCCGAAGTTG174550.17882134451727405No Hit
GACTTTCCTTCG167090.17117879378625792No Hit
TCGAGCGTAGTG162020.16598472780686763No Hit
GGATGGTGGTGC159050.16294205010296442No Hit
TAGCTCGTGACT156150.15997108534157745No Hit
TCGAGGACGGCA153350.15710256764092798No Hit
ACGTGTACACAA145360.1489170474880032No Hit
CGGAGCTAGGGT143760.1472778945162035No Hit
TCACGGGATTTG137970.1413462096995033No Hit
TTAGGGCTTGTA136990.140342228504276No Hit
TCTAGCGTTGTG136790.14013733438280102No Hit
GACTTTCCATCG134730.1380269249316089No Hit
ATGTGCACTACT134230.1375146896279215No Hit
GAGTTTCCCTCG131560.13477935310623074No Hit
GCGATATAGCGC122210.12520055292727622No Hit
TCGCGGGAGTTG122130.12511859527868624No Hit
TCGAAAGTGTTC121310.1242785293806389No Hit
TTAGGGCTAGTA121260.12422730585027013No Hit
GGGTGGTGTTGC121100.12406339055309017No Hit
TCCAAAGTTTTC120780.12373555995873022No Hit
ACGCGCAGTTAC118600.12150221403465314No Hit
AAGAGATGACGA113940.11672818100428649No Hit
TTGGGGCTCGTA111460.11418749389799696No Hit
TAGAGATGGCTC107800.11043793147500512No Hit
ATGCCGAATTTG102060.10455747018867367No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT550.0091590646.00005151
ACACGGG2050.06.00005151
ACGCCCA550.0091590646.00005151
TGAAGGT806.537357E-56.00005151
GCGCGCC650.00126168326.00005151
GTGGGAC550.0091590646.00005151
TCGGGGA1156.823393E-86.00005151
AGGCGCA650.00126168326.00005151
TGACGGT1351.373337E-96.00005151
TCAGGGC1750.06.00005151
CTGCGCT550.0091590646.00005151
CTGCGCA1750.06.00005151
TCCAGGT806.537357E-56.00005151
TACGATT1950.06.00005151
CCGAGCT1351.373337E-96.00005151
TTTGGGC1750.06.00005151
TTGCGGA550.0091590646.00005151
GGACGGG909.1490165E-66.00005151
ACTCGCA5550.06.00005151
TTCGGAC550.0091590646.00005151
Produced by FastQC (version 0.11.2)