##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFJV4 l01n01.3310000000da85.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9761139
Sequences flagged as poor quality	0
Sequence length	301
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.237928278656824	34.0	34.0	34.0	32.0	34.0
2	33.665523255021775	34.0	34.0	34.0	34.0	34.0
3	11.679938785832269	2.0	2.0	34.0	2.0	34.0
4	26.133629999531816	23.0	23.0	34.0	23.0	34.0
5	32.11742041579369	32.0	32.0	34.0	32.0	34.0
6	36.511578925369264	37.0	37.0	37.0	34.0	38.0
7	37.017038380459496	38.0	37.0	38.0	35.0	38.0
8	36.98863831362303	38.0	37.0	38.0	35.0	38.0
9	36.656903769119566	38.0	37.0	38.0	34.0	38.0
10-14	36.93644221232789	38.0	37.0	38.0	35.0	38.0
15-19	36.989415968771674	38.0	37.0	38.0	35.0	38.0
20-24	36.84356622726098	38.0	37.0	38.0	34.2	38.0
25-29	37.124594127795945	38.0	38.0	38.0	36.2	38.0
30-34	36.91977274373411	38.0	37.6	38.0	35.8	38.0
35-39	37.135471526427395	38.0	38.0	38.0	36.2	38.0
40-44	36.972550928738954	38.0	37.4	38.0	35.6	38.0
45-49	36.01226598658211	37.8	36.8	38.0	31.6	38.0
50-54	35.45797782410433	37.6	35.8	38.0	30.0	38.0
55-59	35.605779817293865	38.0	36.4	38.0	30.0	38.0
60-64	33.19701293056066	37.2	33.8	38.0	15.4	38.0
65-69	32.53537317724909	37.0	31.4	38.0	13.0	38.0
70-74	32.995141960379826	37.2	33.4	38.0	17.0	38.0
75-79	32.84171304188989	37.0	32.6	38.0	14.8	38.0
80-84	32.72410678712802	37.0	32.6	38.0	13.0	38.0
85-89	32.394379282991466	37.0	31.4	38.0	11.0	38.0
90-94	32.23890191503266	37.0	31.0	38.0	11.0	38.0
95-99	31.905645335037235	37.0	28.6	38.0	11.0	38.0
100-104	32.08320227793089	37.0	29.8	38.0	11.0	38.0
105-109	31.80544344261464	37.0	28.8	38.0	11.0	38.0
110-114	31.710734843546437	37.0	28.6	38.0	11.0	38.0
115-119	30.682722292961916	36.4	26.0	38.0	11.0	38.0
120-124	31.406667746458687	37.0	27.2	38.0	11.0	38.0
125-129	31.754000450152382	37.0	28.6	38.0	11.0	38.0
130-134	31.295277938363547	37.0	27.0	38.0	11.0	38.0
135-139	31.472293018263542	37.0	27.4	38.0	11.0	38.0
140-144	31.436236324469924	37.0	27.4	38.0	11.0	38.0
145-149	31.180692232740462	37.0	27.0	38.0	11.0	38.0
150-154	30.95303531688259	37.0	26.6	38.0	11.0	38.0
155-159	31.01462513749676	37.0	26.6	38.0	11.0	38.0
160-164	31.064915539057484	37.0	27.2	38.0	11.0	38.0
165-169	31.0374792327002	37.0	27.0	38.0	11.0	38.0
170-174	30.9431380087918	37.0	26.8	38.0	11.0	38.0
175-179	30.641963299569852	36.4	25.4	38.0	11.0	38.0
180-184	30.442966911955665	36.0	24.8	38.0	11.0	38.0
185-189	30.453533424736605	36.0	25.0	38.0	11.0	38.0
190-194	30.048692924053228	35.6	24.2	38.0	11.0	38.0
195-199	29.56534779394085	34.8	22.4	38.0	11.0	38.0
200-204	29.415091517496062	34.2	22.8	38.0	11.0	38.0
205-209	29.19339407009776	34.0	22.2	38.0	11.0	38.0
210-214	28.2131378520478	33.0	19.2	37.2	11.0	38.0
215-219	27.06738221840709	30.4	18.6	36.8	11.0	38.0
220-224	27.277271166817723	30.6	18.2	37.0	11.0	38.0
225-229	26.42031162551829	28.8	16.4	37.0	10.8	38.0
230-234	27.395456985091595	30.8	18.8	37.0	11.0	38.0
235-239	25.88161162339764	28.0	15.6	36.8	10.0	38.0
240-244	26.586780866454212	29.2	16.4	37.0	10.4	38.0
245-249	26.124759784693158	28.4	16.6	36.6	10.0	38.0
250-254	24.92858593653876	26.2	15.4	35.6	9.6	38.0
255-259	24.26602186486638	25.0	14.0	34.8	9.2	38.0
260-264	23.735620914731363	24.6	13.2	34.2	9.0	37.4
265-269	23.026785357733353	23.4	12.2	33.4	8.8	37.0
270-274	23.364864940454186	24.0	13.8	33.4	8.8	37.2
275-279	23.59426038293277	24.2	12.4	34.0	9.0	37.2
280-284	22.882319430140274	23.0	11.0	33.6	8.8	37.0
285-289	23.302572537897476	23.6	10.8	34.0	9.0	37.2
290-294	21.827809500510135	21.6	10.2	32.0	8.6	36.8
295-299	20.54352929509558	19.6	9.6	29.6	8.2	36.0
300-301	15.6636469883279	13.5	8.0	21.5	7.5	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2103	1	-2.4614304068667074
2103	2	-0.513683940158927
2103	3	22.38095531301105
2103	4	7.71077018988159
2103	5	1.682254740112569
2103	6	0.7075647510495386
2103	7	0.17559540738605506
2103	8	-0.41363734856245316
2103	9	-0.6083694407282465
2103	10-14	-0.5374248786522102
2103	15-19	-0.42295139742829946
2103	20-24	-0.2193466972649034
2103	25-29	0.023691109887238326
2103	30-34	-0.013958721779971484
2103	35-39	0.0016905199525751868
2103	40-44	0.26766511137783766
2103	45-49	0.7691714281148805
2103	50-54	-0.9104268721778865
2103	55-59	-0.7419928332537182
2103	60-64	-0.5942923382521528
2103	65-69	-0.26754752110210944
2103	70-74	-0.708917410797028
2103	75-79	-0.6443184210477
2103	80-84	-0.8118573431900344
2103	85-89	-0.5303761703541134
2103	90-94	-0.7575566009282468
2103	95-99	-0.4911777469968399
2103	100-104	-0.6593163205560622
2103	105-109	-0.5184079848581398
2103	110-114	-0.8647526527332445
2103	115-119	-0.3564881088159133
2103	120-124	-0.7906735446995157
2103	125-129	-0.6666125714445812
2103	130-134	-0.9325631290592931
2103	135-139	-0.765299505980714
2103	140-144	-0.5741544022798202
2103	145-149	-0.7489637744751967
2103	150-154	-0.7730995185864415
2103	155-159	-0.9730454233882178
2103	160-164	-0.7805746822378268
2103	165-169	-0.8502822635326837
2103	170-174	-0.7064372483776147
2103	175-179	-1.0358306640878148
2103	180-184	-1.0322104138158323
2103	185-189	-0.8979611701300847
2103	190-194	-0.9998005473691336
2103	195-199	-1.0958636205413015
2103	200-204	-1.14944620313684
2103	205-209	-1.1538080900471286
2103	210-214	-0.5891004936324471
2103	215-219	0.07315938820375933
2103	220-224	-0.27078546274114146
2103	225-229	0.09542660499205624
2103	230-234	-1.7543688353231275
2103	235-239	-6.150119314073589
2103	240-244	-0.7648260736652723
2103	245-249	-0.3729823316406282
2103	250-254	0.33672818664717497
2103	255-259	-0.5554814568843831
2103	260-264	-1.5588574281855898
2103	265-269	-0.9766594444248042
2103	270-274	-1.8529061514643033
2103	275-279	-2.6139689127610097
2103	280-284	-1.7355575676836956
2103	285-289	-2.3657947239857826
2103	290-294	-1.8793517678261686
2103	295-299	-0.44156899253022885
2103	300-301	-0.6687044046028294
2102	1	0.2678334847350854
2102	2	0.10159581769093506
2102	3	-8.154343204158566
2102	4	-2.6901606591525002
2102	5	-0.44293932048297435
2102	6	-0.32461732616813777
2102	7	-0.002035664783086588
2102	8	0.02795110124164779
2102	9	0.23305212612044812
2102	10-14	0.16241006357419252
2102	15-19	0.3236138582323633
2102	20-24	0.3308920758519278
2102	25-29	0.36033181446276785
2102	30-34	0.3482961062922527
2102	35-39	0.13767887619343355
2102	40-44	0.44478325312863376
2102	45-49	0.8841092673434972
2102	50-54	0.4387170515919294
2102	55-59	-0.6878317093246267
2102	60-64	-0.05556270618316006
2102	65-69	-0.6502079384468935
2102	70-74	0.06467345685483394
2102	75-79	0.016807816248672225
2102	80-84	-0.054713830199240476
2102	85-89	0.2143218931808306
2102	90-94	-0.0035773789602160377
2102	95-99	0.32653656868378533
2102	100-104	0.14046193428817588
2102	105-109	0.3760575797509951
2102	110-114	0.1543952149130874
2102	115-119	1.0507243691372103
2102	120-124	0.38513707689313037
2102	125-129	0.2176031886241887
2102	130-134	0.35596438332825286
2102	135-139	0.31600327371223713
2102	140-144	0.2652835504132156
2102	145-149	0.3859060708051061
2102	150-154	0.15619252988293653
2102	155-159	0.26799991996095684
2102	160-164	0.09405968057247804
2102	165-169	0.08695837836661724
2102	170-174	0.24222325499959396
2102	175-179	0.06927629170792216
2102	180-184	0.04822948725754017
2102	185-189	0.14199015805243675
2102	190-194	0.24046181468874295
2102	195-199	0.3462722878397315
2102	200-204	0.09350734349873946
2102	205-209	0.22015815883814582
2102	210-214	0.980111864929551
2102	215-219	1.5380242414384035
2102	220-224	1.4067479622367998
2102	225-229	2.0747938828267785
2102	230-234	0.7044028899223882
2102	235-239	1.1153296386191762
2102	240-244	1.8695009832707186
2102	245-249	2.0078466802067254
2102	250-254	0.7711884580943043
2102	255-259	-6.849059142145933
2102	260-264	-1.228598629080068
2102	265-269	1.4154367972295923
2102	270-274	0.7944974707796106
2102	275-279	0.05370352502276887
2102	280-284	0.059601587219436425
2102	285-289	-0.008518113301740016
2102	290-294	0.3735420891723429
2102	295-299	2.1388913711058493
2102	300-301	0.6473954280089966
2101	1	0.2043673876181913
2101	2	0.07483391460122135
2101	3	-8.154343204158566
2101	4	-2.707050498665847
2101	5	-0.5758766639921937
2101	6	-0.16996499491868633
2101	7	0.06008647305642256
2101	8	0.09828399283748723
2101	9	0.24711436124839992
2101	10-14	0.03863611598650607
2101	15-19	0.21543927350148806
2101	20-24	0.2063499754696494
2101	25-29	0.13782528751369938
2101	30-34	0.09790762328243119
2101	35-39	0.15915284006144503
2101	40-44	0.376475584402975
2101	45-49	0.5599574258791051
2101	50-54	-0.22716482320495146
2101	55-59	0.31031577839777214
2101	60-64	0.31890816934919997
2101	65-69	0.2616830171324054
2101	70-74	0.5817791142644495
2101	75-79	0.4152562133231186
2101	80-84	0.5142411183130449
2101	85-89	0.42740031598198414
2101	90-94	0.3323651030194412
2101	95-99	0.8153356953094857
2101	100-104	0.33091596631665254
2101	105-109	0.6202744613230742
2101	110-114	0.5326554149922949
2101	115-119	1.1640717082322816
2101	120-124	0.5380684303762422
2101	125-129	0.4167099603638533
2101	130-134	0.5369966278735951
2101	135-139	0.4356710047850143
2101	140-144	0.4996151838103593
2101	145-149	0.4808555138835473
2101	150-154	0.5807801184170032
2101	155-159	0.25986357941690486
2101	160-164	0.5544439182633738
2101	165-169	0.4618139953184901
2101	170-174	0.6828436155755853
2101	175-179	0.6139056032113572
2101	180-184	0.4537056442839713
2101	185-189	0.6146976384246763
2101	190-194	0.5930526629064232
2101	195-199	0.7685927175576879
2101	200-204	0.5938373111205202
2101	205-209	0.6746346645268559
2101	210-214	1.2931906334425207
2101	215-219	1.7630972011806634
2101	220-224	1.5622744885854765
2101	225-229	2.188233491138675
2101	230-234	0.6171751890220847
2101	235-239	0.9978584372366122
2101	240-244	1.42459269933304
2101	245-249	-5.484928235768976
2101	250-254	-0.2379256288585765
2101	255-259	-6.688021007659504
2101	260-264	-2.8359274551008546
2101	265-269	0.7053074890178763
2101	270-274	0.8124986710937314
2101	275-279	-0.10090686107568558
2101	280-284	0.5826738032044467
2101	285-289	0.800256867260849
2101	290-294	0.8404670436351154
2101	295-299	1.8222406582569945
2101	300-301	3.147996353294028
2107	1	0.1303769874037286
2107	2	0.08121167551320951
2107	3	-8.154343204158566
2107	4	-2.7095934300662208
2107	5	-0.5781969886404354
2107	6	-0.24738716543581774
2107	7	0.05744580709432512
2107	8	-0.011744546040347359
2107	9	0.23075963525226229
2107	10-14	0.07731419664267491
2107	15-19	0.16186850309868817
2107	20-24	0.20682889368088553
2107	25-29	0.1764750887445672
2107	30-34	0.1771080348639842
2107	35-39	0.14800368710698564
2107	40-44	0.33481585385150225
2107	45-49	-8.63103390149898
2107	50-54	-1.4124919527387334
2107	55-59	0.30881244871011404
2107	60-64	0.3313693176405934
2107	65-69	0.7932476408295202
2107	70-74	0.6382858385313739
2107	75-79	0.4747857245295748
2107	80-84	0.5966030639808864
2107	85-89	0.5386354267840616
2107	90-94	0.576762596780803
2107	95-99	-3.7434034927061752
2107	100-104	0.4678987707798896
2107	105-109	0.655063231360625
2107	110-114	0.5750662618867644
2107	115-119	1.2653588746068358
2107	120-124	0.40254687095760744
2107	125-129	0.5616789249678931
2107	130-134	0.5011282284231022
2107	135-139	0.45919298168916
2107	140-144	0.5539363830960333
2107	145-149	0.3836946921345614
2107	150-154	0.47671511702788294
2107	155-159	0.5814684436075161
2107	160-164	0.4839628922855894
2107	165-169	0.4426787222711184
2107	170-174	0.7178555757353919
2107	175-179	0.5408645010178716
2107	180-184	0.6896483197227852
2107	185-189	0.4932049064462305
2107	190-194	0.6139308701364605
2107	195-199	0.6572214179271967
2107	200-204	0.6725806966218393
2107	205-209	0.5515727052549444
2107	210-214	1.473086723728148
2107	215-219	-3.3467011208089232
2107	220-224	-8.155966482258332
2107	225-229	1.5488027826766562
2107	230-234	0.5076577682454584
2107	235-239	2.059364394057667
2107	240-244	1.5910827614351568
2107	245-249	-1.7585350183021795
2107	250-254	-4.886214115681764
2107	255-259	-5.850274479490544
2107	260-264	-0.9888399837288588
2107	265-269	1.5127195103406557
2107	270-274	-0.1780245897584969
2107	275-279	0.15939933412132135
2107	280-284	0.29656229172345405
2107	285-289	0.9666974677937539
2107	290-294	0.7861230705605458
2107	295-299	1.4924303849824447
2107	300-301	0.19418310694474883
2106	1	0.3319501558751057
2106	2	0.10680063538134732
2106	3	-8.154343204158566
2106	4	-2.700649075067968
2106	5	-0.621266420142824
2106	6	-0.17096623813260692
2106	7	0.03128747531533094
2106	8	0.11264073021339982
2106	9	0.3351147593296986
2106	10-14	0.012950693843187366
2106	15-19	0.039682688350005435
2106	20-24	0.10001235684880072
2106	25-29	0.02738231796263335
2106	30-34	-0.03281487410747275
2106	35-39	0.03437321278964589
2106	40-44	-0.044956473893705606
2106	45-49	0.8402063081300071
2106	50-54	0.3738151935635017
2106	55-59	0.2951502834970725
2106	60-64	0.4610481235928461
2106	65-69	0.7154286289260483
2106	70-74	0.3670766721244263
2106	75-79	0.2383072991301063
2106	80-84	0.37575909622784565
2106	85-89	0.14070749186483056
2106	90-94	0.18688964223764515
2106	95-99	0.40348359692573155
2106	100-104	0.18922980706351922
2106	105-109	0.14452749083269723
2106	110-114	0.2487932337733234
2106	115-119	1.2949568665811064
2106	120-124	0.5592402271154171
2106	125-129	0.47096455022938244
2106	130-134	0.5431218028698162
2106	135-139	0.519939429685703
2106	140-144	0.3889259612045457
2106	145-149	0.3491962496754297
2106	150-154	0.3231380693619563
2106	155-159	0.3269065024296225
2106	160-164	0.34474702154138726
2106	165-169	0.3989846274253992
2106	170-174	0.23540068928189228
2106	175-179	0.34698217260948283
2106	180-184	0.579005275486896
2106	185-189	0.1759987827088807
2106	190-194	0.42215183543364176
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2115	2	-0.15413679383458856
2115	3	23.584906019845576
2115	4	7.683152076945799
2115	5	1.6908716873050125
2115	6	0.7866739273822816
2115	7	0.05435386817686805
2115	8	-0.14026979299517706
2115	9	-0.7215225251570274
2115	10-14	-0.279869164006584
2115	15-19	-0.2909611867385635
2115	20-24	-0.46427193027898284
2115	25-29	-0.3018990661881844
2115	30-34	-0.21575000076272488
2115	35-39	-0.1801977824010379
2115	40-44	0.023350956599628603
2115	45-49	0.4224518056073876
2115	50-54	-0.4655883330822874
2115	55-59	-0.5704352802946318
2115	60-64	-1.1432783185128415
2115	65-69	-0.9150964420829588
2115	70-74	-1.3473371580075657
2115	75-79	-1.3187228936827395
2115	80-84	-1.4328264359854614
2115	85-89	-1.4649302964018958
2115	90-94	-1.2511929318674682
2115	95-99	-1.1874387550754797
2115	100-104	-1.8203039596660382
2115	105-109	-1.6746399475974165
2115	110-114	-2.934303838852202
2115	115-119	-1.2770412410479786
2115	120-124	-1.0962136148056167
2115	125-129	-1.0957994432526519
2115	130-134	-1.2233551478355373
2115	135-139	-1.173599990258122
2115	140-144	-1.114488338223417
2115	145-149	-1.0777036041652366
2115	150-154	-1.4059703705211604
2115	155-159	-1.3149834121539108
2115	160-164	-1.3188335273928686
2115	165-169	-1.1817152301947331
2115	170-174	-1.117207666516375
2115	175-179	-1.029906480404236
2115	180-184	-1.6575349482452317
2115	185-189	-1.2132791884686753
2115	190-194	-1.0691136389549492
2115	195-199	-0.9558941930421376
2115	200-204	-0.9944972343527034
2115	205-209	-1.4596286444360196
2115	210-214	-0.9627513613723906
2115	215-219	0.1997972444876055
2115	220-224	0.03392086602593736
2115	225-229	0.3285823537944168
2115	230-234	-1.1182112923499687
2115	235-239	0.5908020508043492
2115	240-244	0.17517108263154668
2115	245-249	0.1505567023912171
2115	250-254	0.9350741082680898
2115	255-259	0.8673805019170437
2115	260-264	0.10953712237472502
2115	265-269	-0.6880351369802504
2115	270-274	-0.803950574804901
2115	275-279	-0.300429876037537
2115	280-284	-0.692295012794709
2115	285-289	-0.6085855562258686
2115	290-294	-0.38258579046705066
2115	295-299	-4.680104064151713
2115	300-301	-1.4728142437485499
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	23.0
12	263.0
13	964.0
14	1853.0
15	2824.0
16	4656.0
17	8937.0
18	31383.0
19	144652.0
20	396776.0
21	506289.0
22	403946.0
23	348154.0
24	326127.0
25	314318.0
26	322115.0
27	344131.0
28	385845.0
29	445881.0
30	528859.0
31	608792.0
32	675229.0
33	799845.0
34	997552.0
35	1166889.0
36	903816.0
37	91017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.8550459121625047	89.85274157042534	4.539275590686702	2.752936926725457
2	87.48031351669103	5.334500410249255	3.945994417249872	3.2391916558098393
3	3.6341140283931166	4.193122086434804	2.3086504184300862	89.864113466742
4	2.6397329246105397	4.259308263103312	89.63625044167489	3.464708370611257
5	3.0835438364313834	3.603206552022259	3.7224549307206876	89.59079468082567
6	3.931641584040551	4.292419153133666	16.44081699891785	75.33512226390793
7	4.32356305959786	76.22521306171339	5.023932145623579	14.427291733065168
8	7.437666854247235	78.09642911549564	8.778391538118656	5.6875124921384685
9	8.264363410868341	16.19839651909475	71.26603770318198	4.271202366854934
10-14	37.274580353788636	22.138182849358053	23.14326432601769	17.44397247083563
15-19	3.6937656558317626	39.143767955768276	48.79625420762884	8.366212180771116
20-24	20.01512118616485	49.91186581811815	24.980369606456787	5.092643389260208
25-29	37.504985842328445	4.034002589247013	52.33104661249062	6.129964955933934
30-34	24.04671012266089	17.170203190426854	39.151105214258294	19.63198147265396
35-39	3.874615452151639	88.7670485995538	3.4441861754043255	3.9141497728902332
40-44	37.92409266992305	3.6453409791623703	21.0959397258865	37.334626625028086
45-49	20.491514874671388	21.180634277854715	53.80600520169596	4.521845645777937
50-54	30.53943255063641	32.04778729301544	27.43356338709168	9.979216769256473
55-59	34.966782869645264	8.273467534759419	37.53612872728305	19.223620868312263
60-64	29.000579379202264	18.27237469272792	28.4885406899405	24.238505238129317
65-69	29.92024514850043	19.7738099475014	29.690487068893184	20.61545783510498
70-74	28.396828661960598	11.40271624128959	33.13684504898434	27.06361004776547
75-79	21.773861185184455	11.802581578086038	29.148787099599865	37.27477013712964
80-84	33.63423069619383	17.438843929464806	28.254995218355738	20.671930155985624
85-89	33.07592574831092	19.859701919635675	27.56436645171587	19.500005880337532
90-94	29.405229064659792	21.102588301843745	27.503301189627276	21.988881443869182
95-99	23.75279923976621	23.497903584598973	24.991347949142853	27.757949226491963
100-104	23.52977183794258	13.58650942572009	33.342359959350986	29.54135877698635
105-109	22.42678032917955	19.554494774249477	30.959062145496535	27.059662751074438
110-114	17.2297113723704	30.607550016528084	27.65210436053705	24.51063425056447
115-119	31.46275763592784	15.368063406143476	28.581207718976486	24.5879712389522
120-124	20.207482568299074	21.07014725133651	33.13750036786595	25.584869812498468
125-129	24.590991826156646	16.884654800219202	39.50119435975191	19.02315901387223
130-134	24.700106178148886	21.406004369596555	25.680419255220226	28.21347019703433
135-139	28.3303679172141	22.864232452179355	32.01242934333322	16.792970287273327
140-144	30.13178017754393	21.774337447896322	34.739143096244696	13.354739278315048
145-149	30.857103919616097	24.02515468640807	27.994386801806787	17.123354592169047
150-154	26.17558614695925	25.674239975364404	29.103135412095693	19.04703846558065
155-159	19.144191684120784	26.513381972882755	32.746985994206376	21.59544034879008
160-164	15.318863759843065	32.46481703678511	35.87693465227158	16.33938455110025
165-169	17.7725563509153	38.947431497360625	31.65545435676688	11.624557794957198
170-174	21.440051901338116	37.05562235195007	30.55243976409332	10.951885982618494
175-179	25.49838919373385	28.900335233501394	29.337524074809075	16.263751497955678
180-184	29.185648828766663	31.71640912599349	23.057639858337005	16.040302186902842
185-189	25.95623174265132	27.652120070426218	29.2198573567959	17.171790830126568
190-194	24.812826142785717	22.871761643221383	30.321177279028138	21.99423493496476
195-199	22.544021195559683	28.734272275689797	26.16218099470115	22.559525534049367
200-204	22.588266958698178	26.046306238215	28.2966933447025	23.068733458384322
205-209	21.70073185106779	30.60843411819051	33.53508233004366	14.155751700698044
210-214	23.328252983591362	35.90011575493393	31.222948469435792	9.548682792038921
215-219	18.644885984000144	42.24327800768493	27.892507788128164	11.219328220186767
220-224	26.703604978886176	38.40560410009529	23.419592734003686	11.471198187014856
225-229	25.658776617334855	34.903597518764926	25.84338212668382	13.594243737216399
230-234	23.201895254270948	32.581608203337915	31.051360523630706	13.165136018760434
235-239	21.449746796967034	22.55448057854724	40.495384811137306	15.500387813348421
240-244	23.262066035531305	16.194456405138784	46.039778759425516	14.503698799904397
245-249	29.48023791076021	17.194972840771964	42.59605359579451	10.72873565267332
250-254	27.356102121307774	18.953516230249864	42.87102582650801	10.819355821934353
255-259	29.014522362902984	22.329759310460783	39.6177421429557	9.037976183680534
260-264	23.359880440182238	29.529988252395544	36.409619819982076	10.700511487440144
265-269	25.15251858015826	33.54166490372342	28.04828731762911	13.257529198489202
270-274	29.896414752417726	35.04626662933496	24.387479780791978	10.669838837455343
275-279	36.15278094083077	29.42222009132336	24.00017866767393	10.424820300171938
280-284	33.02048459713564	28.111885303549105	25.312986527494385	13.55464357182087
285-289	35.47858093199984	25.14867783360118	27.01216733006261	12.36057390433637
290-294	30.54919103190724	25.227371518836073	30.799907674708866	13.423529774547827
295-299	34.50890106164864	22.671587813676254	27.57475331516127	15.244757809513828
300-301	36.02076560942324	20.61886937579723	32.28735908790972	11.073005926869804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.25
2	0.0
3	0.0
4	0.0
5	0.75
6	24.75
7	67.25
8	22.25
9	9.0
10	4.25
11	7.25
12	7.75
13	18.5
14	25.0
15	28.25
16	44.5
17	67.0
18	146.25
19	237.25
20	250.75
21	315.0
22	661.5
23	1111.75
24	1274.5
25	1521.0
26	1639.75
27	1920.5
28	2652.75
29	4388.0
30	8828.5
31	6403.75
32	6882.25
33	8545.5
34	14549.25
35	23603.75
36	36494.5
37	76918.0
38	187838.75
39	349572.25
40	274863.5
41	241787.5
42	333874.75
43	357178.25
44	259626.75
45	213664.25
46	201721.75
47	169414.0
48	136782.5
49	130512.0
50	63343.75
51	35501.25
52	25177.0
53	17194.75
54	12382.25
55	9373.5
56	6885.0
57	5561.0
58	4446.5
59	2782.75
60	1848.25
61	1559.0
62	1245.75
63	1050.0
64	899.75
65	574.25
66	306.25
67	164.25
68	80.25
69	42.5
70	32.0
71	23.25
72	19.0
73	15.25
74	9.75
75	11.75
76	9.75
77	8.75
78	7.25
79	10.5
80	10.25
81	8.5
82	7.25
83	9.5
84	7.0
85	6.25
86	7.5
87	5.75
88	3.5
89	3.75
90	5.75
91	9.5
92	7.25
93	7.5
94	5.75
95	3.5
96	3.0
97	3.5
98	7.25
99	11.25
100	39.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	68.99142610303983
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.9691430477529313
50-54	2.048941214749631E-6
55-59	8.195764858998525E-6
60-64	1.639152971799705E-5
65-69	2.4587294576995575E-5
70-74	0.005011710211277598
75-79	0.046281484158764666
80-84	0.07561822447154988
85-89	0.17211515992139853
90-94	0.20286771861357575
95-99	0.29468282338772145
100-104	0.35200400281155714
105-109	0.48535729283232215
110-114	0.6030669166784737
115-119	0.6598922523283399
120-124	0.43975400821563954
125-129	0.31060719450875557
130-134	0.22188599096888179
135-139	0.1514515877706485
140-144	0.29468692127015095
145-149	0.2000463265608655
150-154	0.14282964313898205
155-159	0.07555880517632214
160-164	0.036192497617337484
165-169	0.01911047470996981
170-174	0.004771984089151891
175-179	2.4587294576995575E-5
180-184	1.2293647288497788E-5
185-189	1.0244706073748155E-5
190-194	2.048941214749631E-6
195-199	2.048941214749631E-6
200-204	6.146823644248894E-6
205-209	0.0
210-214	0.0
215-219	2.048941214749631E-6
220-224	0.0
225-229	2.048941214749631E-6
230-234	2.048941214749631E-6
235-239	0.0
240-244	0.0
245-249	0.0
250-254	1.102172605061766
255-259	2.048941214749631E-6
260-264	0.0
265-269	4.097882429499262E-6
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	9761139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.361042961125623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.72141706923279	14.188528303194106
2	5.6817661543022036	1.8591924029121576
3	1.7037270233831971	0.8362425307080951
4	0.8259688120106787	0.5405484487142643
5	0.653103525509964	0.5342727419465564
6	0.47161684715997987	0.4629686098545029
7	0.3700215060954882	0.4237756430438082
8	0.37795609852938217	0.4947004772366921
9	0.25072557154927017	0.3691918662813347
>10	2.6478507233724495	9.138769395633386
>50	0.16818477388164868	1.8309869744866567
>100	0.09315797986295589	3.0272387486091503
>500	0.014128097807168432	1.6853227395248864
>1k	0.012817704825385457	4.2975679335665244
>5k	0.002793215480848695	3.4209346037211064
>10k+	0.004764896996741892	56.88975858056674
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	963605	9.871849996194092	No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	168025	1.721366738041534	No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	144923	1.484693538325804	No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG	123624	1.266491543661042	No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	105085	1.076564937759825	No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	78266	0.8018121655679732	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	60434	0.619128566860896	No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	42552	0.4359327328501315	No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG	38434	0.3937450332384366	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	37513	0.3843096589445146	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG	32323	0.33113963442176164	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	31339	0.32105884364519344	No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	28884	0.2959080902341418	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	26452	0.2709929650627862	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	23926	0.24511483752049837	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	23225	0.23793329856280093	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG	18947	0.19410644597930632	No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG	18578	0.19032614943809323	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	18316	0.18764203644677122	No Hit
AGCTCCAATAGCGTATATTATTGATGTTGCAGTTGAAACGTTCGTAGTCG	15446	0.15823973001511402	No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	15349	0.15724599352596044	No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	13627	0.1396046096669661	No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	13411	0.13739175315503652	No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	12772	0.13084538597391146	No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	12729	0.13040486361274028	No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG	12717	0.13028192713985529	No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	12498	0.12803833650970445	No Hit
TTCCCACGCCAATGAGGCCGCAACCAAAGACGGTAAGGTGATCGCTGATA	12155	0.12452440232640884	No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	11098	0.11369574800645704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.175858882861928E-4	0.0	0.0
2	3.175858882861928E-4	0.0	0.0
3	3.175858882861928E-4	0.0	0.0
4	3.2783059435994097E-4	0.0	0.0
5	3.380753004336891E-4	0.0	0.0
6	3.380753004336891E-4	0.0	0.0
7	3.483200065074373E-4	0.0	0.0
8	3.5856471258118543E-4	0.0	0.0
9	3.6880941865493364E-4	0.0	0.0
10-14	4.2003294902367446E-4	0.0	0.0
15-19	4.6101177331866707E-4	0.0	0.0
20-24	5.716545989151471E-4	0.0	0.0
25-29	6.863953069411264E-4	0.0	0.0
30-34	9.650513121470764E-4	0.0	0.0
35-39	0.0012805882592185195	0.0	0.0
40-44	0.00144450355639849	0.0	0.0
45-49	0.0017211106203896902	0.0	7.376188373098673E-5
50-54	0.0022497374537950947	0.0	2.0694306268971276E-4
55-59	0.0030426777039032024	0.0	2.2333459240770977E-4
60-64	0.0039687991329700354	0.0	2.4177506334045648E-4
65-69	0.005271925745550801	0.0	2.4587294576995574E-4
70-74	0.006599639652708563	0.0	2.4587294576995574E-4
75-79	0.008572770042512456	0.0	2.4587294576995574E-4
80-84	0.011121652913660999	6.146823644248894E-6	2.4587294576995574E-4
85-89	0.014174575323637948	1.0244706073748155E-5	2.4587294576995574E-4
90-94	0.017899550452052777	1.0244706073748155E-5	2.4587294576995574E-4
95-99	0.02238468277113972	1.0244706073748155E-5	2.4792188698470537E-4
100-104	0.028533555356603364	1.0244706073748155E-5	6.863953069411265E-4
105-109	0.03544463407395387	1.0244706073748155E-5	0.0012805882592185195
110-114	0.04381250999499136	1.0244706073748155E-5	0.001288784024077518
115-119	0.054534619371776186	1.0244706073748155E-5	0.0012949308477217668
120-124	0.06730362102209589	1.0244706073748155E-5	0.0014178673206067449
125-129	0.08212771071080946	1.0244706073748155E-5	0.001594076265075213
130-134	0.09836761877891505	1.0244706073748155E-5	0.0015981741475047124
135-139	0.11578157016307215	1.0244706073748155E-5	0.0016125167360079597
140-144	0.1341032025053634	1.0244706073748155E-5	0.0016493976778734533
145-149	0.15310713227216616	6.146823644248893E-5	0.0016903765021684457
150-154	0.172274977336149	7.27374131236119E-4	0.0016985722670274442
155-159	0.19267218712898154	7.27374131236119E-4	0.0017006212082421941
160-164	0.21463273906866812	7.27374131236119E-4	0.0017006212082421941
165-169	0.23614252394110974	7.27374131236119E-4	0.001710865914315942
170-174	0.25819118035303046	7.27374131236119E-4	0.001710865914315942
175-179	0.28023778782373654	7.27374131236119E-4	0.0017211106203896902
180-184	0.3029748884838132	7.27374131236119E-4	0.0050711295065053365
185-189	0.32650083151156845	7.27374131236119E-4	0.008181422270495278
190-194	0.35090986820288084	7.27374131236119E-4	0.018643316113006896
195-199	0.3747349566479895	7.27374131236119E-4	0.01866380552515439
200-204	0.39888582674624345	7.335209548803679E-4	0.01867200129001339
205-209	0.4201067109074053	7.949891913228569E-4	0.01877854623318037
210-214	0.4409423941201943	8.912894284160896E-4	0.02311205690237584
215-219	0.46169407074317864	9.056320169193371E-4	0.02454631575270058
220-224	0.48236174077635824	9.220235466373339E-4	0.02454631575270058
225-229	0.5037158061164788	9.220235466373339E-4	0.02455656045877433
230-234	0.5237585490791596	9.220235466373339E-4	0.02455860939998908
235-239	0.5433218397975892	9.220235466373339E-4	0.024566805164848077
240-244	0.5623606015650429	9.220235466373339E-4	0.024566805164848077
245-249	0.5820693671097195	9.220235466373339E-4	0.024566805164848077
250-254	0.6040258211669765	9.220235466373339E-4	0.024566805164848077
255-259	0.6269801095958167	9.220235466373339E-4	0.024575000929707075
260-264	0.6509383792198842	9.220235466373339E-4	0.024577049870921824
265-269	0.6775828107764883	9.220235466373339E-4	0.024577049870921824
270-274	0.7055201242395994	9.220235466373339E-4	0.024587294576995575
275-279	0.733969673006398	9.220235466373339E-4	0.024587294576995575
280-284	0.7635994119128925	9.240724878520837E-4	0.024593441400639824
285-289	0.7916350745543117	9.384150763553312E-4	0.024597539283069323
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCCA	213455	0.0	936.35956	1
GCTCCAA	203235	0.0	930.19965	2
CTCCAAT	174225	0.0	928.3331	3
AGCTCTC	34695	0.0	897.31683	1
CTCCAGT	13015	0.0	837.9737	3
CTCTCTA	15980	0.0	774.1001	3
GCTCCAG	12940	0.0	768.2599	2
CTCTCAT	15085	0.0	745.1394	3
AGCTCTA	3740	0.0	649.42145	1
AGCTCCG	1990	0.0	641.0099	1
GCTCTCA	27670	0.0	628.9105	2
CTCCAAA	26270	0.0	615.3026	3
TCCCACG	2230	0.0	599.46246	2
GCTCTCT	21405	0.0	580.32684	2
CCCACGC	2735	0.0	488.7756	3
AACTCCA	6380	0.0	467.7536	1
TTCCCAC	3050	0.0	432.12234	1
CTCTCAA	14460	0.0	425.78265	3
CTCCGCA	2715	0.0	343.27637	3
AATCGTC	7015	0.0	322.07877	2
>>END_MODULE