FastQCFastQC Report
Thu 2 Jul 2015
000000000-AFJV4 l01n01.3310000000da85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJV4 l01n01.3310000000da85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9761139
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG9636059.871849996194092No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1680251.721366738041534No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1449231.484693538325804No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG1236241.266491543661042No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1050851.076564937759825No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG782660.8018121655679732No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG604340.619128566860896No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG425520.4359327328501315No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG384340.3937450332384366No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG375130.3843096589445146No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG323230.33113963442176164No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG313390.32105884364519344No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG288840.2959080902341418No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG264520.2709929650627862No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG239260.24511483752049837No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG232250.23793329856280093No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG189470.19410644597930632No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG185780.19032614943809323No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG183160.18764203644677122No Hit
AGCTCCAATAGCGTATATTATTGATGTTGCAGTTGAAACGTTCGTAGTCG154460.15823973001511402No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG153490.15724599352596044No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG136270.1396046096669661No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG134110.13739175315503652No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG127720.13084538597391146No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG127290.13040486361274028No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG127170.13028192713985529No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG124980.12803833650970445No Hit
TTCCCACGCCAATGAGGCCGCAACCAAAGACGGTAAGGTGATCGCTGATA121550.12452440232640884No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG110980.11369574800645704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA2134550.0936.359561
GCTCCAA2032350.0930.199652
CTCCAAT1742250.0928.33313
AGCTCTC346950.0897.316831
CTCCAGT130150.0837.97373
CTCTCTA159800.0774.10013
GCTCCAG129400.0768.25992
CTCTCAT150850.0745.13943
AGCTCTA37400.0649.421451
AGCTCCG19900.0641.00991
GCTCTCA276700.0628.91052
CTCCAAA262700.0615.30263
TCCCACG22300.0599.462462
GCTCTCT214050.0580.326842
CCCACGC27350.0488.77563
AACTCCA63800.0467.75361
TTCCCAC30500.0432.122341
CTCTCAA144600.0425.782653
CTCCGCA27150.0343.276373
AATCGTC70150.0322.078772
Produced by FastQC (version 0.11.2)