##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFJR0 l01n03.3330000000edf3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20709823
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.046629128602405	23.0	21.0	32.0	12.0	34.0
2	24.403394804484808	27.0	18.0	33.0	11.0	34.0
3	14.565565432403744	11.0	2.0	28.0	2.0	34.0
4	15.006631490766484	11.0	2.0	27.0	2.0	34.0
5	21.075938360265077	23.0	12.0	27.0	11.0	34.0
6	15.296239180798407	11.0	2.0	26.0	2.0	34.0
7	16.161514803868677	11.0	2.0	31.0	2.0	36.0
8	20.51630344691985	25.0	11.0	31.0	2.0	37.0
9	25.525093237156106	27.0	21.0	34.0	11.0	37.0
10-14	25.030357990022416	26.6	17.2	34.4	9.2	37.0
15-19	26.416843717109508	29.8	17.2	36.4	9.2	37.2
20-24	26.476088569178017	29.8	17.2	35.6	10.8	37.6
25-29	25.80365052854387	28.8	17.2	34.8	11.0	36.8
30-34	25.230221716525534	27.8	14.2	34.6	11.0	37.0
35-39	23.193989924491387	24.6	12.2	32.0	8.2	36.2
40-44	24.80006073446403	26.4	11.0	34.2	11.0	37.0
45-49	25.08828996751928	27.6	15.4	34.8	10.4	36.6
50-54	13.93260205072733	14.2	7.4	20.8	5.0	22.8
55-59	2.0	2.0	2.0	2.0	2.0	2.0
60-64	2.0	2.0	2.0	2.0	2.0	2.0
65-69	2.0	2.0	2.0	2.0	2.0	2.0
70-74	2.0	2.0	2.0	2.0	2.0	2.0
75-79	2.0	2.0	2.0	2.0	2.0	2.0
80-84	2.0	2.0	2.0	2.0	2.0	2.0
85-89	2.0	2.0	2.0	2.0	2.0	2.0
90-94	2.0	2.0	2.0	2.0	2.0	2.0
95-99	2.0	2.0	2.0	2.0	2.0	2.0
100-104	2.0	2.0	2.0	2.0	2.0	2.0
105-109	2.0	2.0	2.0	2.0	2.0	2.0
110-114	2.0	2.0	2.0	2.0	2.0	2.0
115-119	2.0	2.0	2.0	2.0	2.0	2.0
120-124	2.0	2.0	2.0	2.0	2.0	2.0
125-129	2.0	2.0	2.0	2.0	2.0	2.0
130-134	2.0	2.0	2.0	2.0	2.0	2.0
135-139	2.0	2.0	2.0	2.0	2.0	2.0
140-144	2.0	2.0	2.0	2.0	2.0	2.0
145-149	2.0	2.0	2.0	2.0	2.0	2.0
150-154	2.0	2.0	2.0	2.0	2.0	2.0
155-159	2.0	2.0	2.0	2.0	2.0	2.0
160-164	2.0	2.0	2.0	2.0	2.0	2.0
165-169	2.0	2.0	2.0	2.0	2.0	2.0
170-174	2.0	2.0	2.0	2.0	2.0	2.0
175-179	2.0	2.0	2.0	2.0	2.0	2.0
180-184	2.0	2.0	2.0	2.0	2.0	2.0
185-189	2.0	2.0	2.0	2.0	2.0	2.0
190-194	2.0	2.0	2.0	2.0	2.0	2.0
195-199	2.0	2.0	2.0	2.0	2.0	2.0
200-204	2.0	2.0	2.0	2.0	2.0	2.0
205-209	2.0	2.0	2.0	2.0	2.0	2.0
210-214	2.0	2.0	2.0	2.0	2.0	2.0
215-219	2.0	2.0	2.0	2.0	2.0	2.0
220-224	2.0	2.0	2.0	2.0	2.0	2.0
225-229	2.0	2.0	2.0	2.0	2.0	2.0
230-234	2.0	2.0	2.0	2.0	2.0	2.0
235-239	2.0	2.0	2.0	2.0	2.0	2.0
240-244	2.0	2.0	2.0	2.0	2.0	2.0
245-249	2.0	2.0	2.0	2.0	2.0	2.0
250-254	2.0	2.0	2.0	2.0	2.0	2.0
255-259	2.0	2.0	2.0	2.0	2.0	2.0
260-264	2.0	2.0	2.0	2.0	2.0	2.0
265-269	2.0	2.0	2.0	2.0	2.0	2.0
270-274	2.0	2.0	2.0	2.0	2.0	2.0
275-279	2.0	2.0	2.0	2.0	2.0	2.0
280-284	2.0	2.0	2.0	2.0	2.0	2.0
285-289	2.0	2.0	2.0	2.0	2.0	2.0
290-294	2.0	2.0	2.0	2.0	2.0	2.0
295-299	2.0	2.0	2.0	2.0	2.0	2.0
300-301	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	2.1094720653006505
1101	2	3.4417899224378985
1101	3	11.651699397983945
1101	4	10.431734241830743
1101	5	6.526578300741093
1101	6	14.182833757426174
1101	7	14.510044129378542
1101	8	8.557548895157762
1101	9	2.8206142442025666
1101	10-14	4.427189065186479
1101	15-19	4.283723440663984
1101	20-24	4.295790599829559
1101	25-29	3.4394230954545293
1101	30-34	3.197878429742211
1101	35-39	5.694410693618362
1101	40-44	3.6988839724966347
1101	45-49	3.45512102996711
1101	50-54	2.323732479852044
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-154	0.0
1101	155-159	0.0
1101	160-164	0.0
1101	165-169	0.0
1101	170-174	0.0
1101	175-179	0.0
1101	180-184	0.0
1101	185-189	0.0
1101	190-194	0.0
1101	195-199	0.0
1101	200-204	0.0
1101	205-209	0.0
1101	210-214	0.0
1101	215-219	0.0
1101	220-224	0.0
1101	225-229	0.0
1101	230-234	0.0
1101	235-239	0.0
1101	240-244	0.0
1101	245-249	0.0
1101	250-254	0.0
1101	255-259	0.0
1101	260-264	0.0
1101	265-269	0.0
1101	270-274	0.0
1101	275-279	0.0
1101	280-284	0.0
1101	285-289	0.0
1101	290-294	0.0
1101	295-299	0.0
1101	300-301	0.0
1102	1	0.6431376189559472
1102	2	1.0737826735975133
1102	3	9.047031420031102
1102	4	8.866611408628344
1102	5	3.118857435546044
1102	6	10.181429906996588
1102	7	11.653124216489356
1102	8	6.166218269028903
1102	9	2.9306397972587703
1102	10-14	2.4760562264472803
1102	15-19	3.681910135422779
1102	20-24	2.7249621699452646
1102	25-29	1.3191930657501558
1102	30-34	0.9755012862292496
1102	35-39	3.281024471689527
1102	40-44	1.2711540104285888
1102	45-49	2.066782947534307
1102	50-54	1.927676267017695
1102	55-59	0.0
1102	60-64	0.0
1102	65-69	0.0
1102	70-74	0.0
1102	75-79	0.0
1102	80-84	0.0
1102	85-89	0.0
1102	90-94	0.0
1102	95-99	0.0
1102	100-104	0.0
1102	105-109	0.0
1102	110-114	0.0
1102	115-119	0.0
1102	120-124	0.0
1102	125-129	0.0
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-254	0.0
1102	255-259	0.0
1102	260-264	0.0
1102	265-269	0.0
1102	270-274	0.0
1102	275-279	0.0
1102	280-284	0.0
1102	285-289	0.0
1102	290-294	0.0
1102	295-299	0.0
1102	300-301	0.0
1103	1	0.012694997537717967
1103	2	-0.11234452533275174
1103	3	9.524973961056007
1103	4	9.375517639146697
1103	5	2.468850263553069
1103	6	8.809392563644384
1103	7	10.566100211918378
1103	8	5.796286257569285
1103	9	4.8250156106944
1103	10-14	2.9394070040802482
1103	15-19	4.02632079419244
1103	20-24	1.2966930368671008
1103	25-29	0.7988057687710892
1103	30-34	0.01650611974291749
1103	35-39	1.8279533826591496
1103	40-44	0.5850970692258102
1103	45-49	1.289290354671813
1103	50-54	2.8919537043902785
1103	55-59	0.0
1103	60-64	0.0
1103	65-69	0.0
1103	70-74	0.0
1103	75-79	0.0
1103	80-84	0.0
1103	85-89	0.0
1103	90-94	0.0
1103	95-99	0.0
1103	100-104	0.0
1103	105-109	0.0
1103	110-114	0.0
1103	115-119	0.0
1103	120-124	0.0
1103	125-129	0.0
1103	130-134	0.0
1103	135-139	0.0
1103	140-144	0.0
1103	145-149	0.0
1103	150-154	0.0
1103	155-159	0.0
1103	160-164	0.0
1103	165-169	0.0
1103	170-174	0.0
1103	175-179	0.0
1103	180-184	0.0
1103	185-189	0.0
1103	190-194	0.0
1103	195-199	0.0
1103	200-204	0.0
1103	205-209	0.0
1103	210-214	0.0
1103	215-219	0.0
1103	220-224	0.0
1103	225-229	0.0
1103	230-234	0.0
1103	235-239	0.0
1103	240-244	0.0
1103	245-249	0.0
1103	250-254	0.0
1103	255-259	0.0
1103	260-264	0.0
1103	265-269	0.0
1103	270-274	0.0
1103	275-279	0.0
1103	280-284	0.0
1103	285-289	0.0
1103	290-294	0.0
1103	295-299	0.0
1103	300-301	0.0
2116	1	-0.5117003345095448
2116	2	-1.528041526601747
2116	3	7.84166402549325
2116	4	8.216875665602252
2116	5	0.6211545582056957
2116	6	7.362321536145604
2116	7	9.602533544459448
2116	8	6.191964524336896
2116	9	5.546365592072185
2116	10-14	3.2999732391663166
2116	15-19	4.0867640316960845
2116	20-24	1.3858224585504786
2116	25-29	1.012512036698812
2116	30-34	-0.5891477275380304
2116	35-39	0.4875004388527131
2116	40-44	0.44465221658348497
2116	45-49	0.9571445985509008
2116	50-54	2.8530078308908955
2116	55-59	0.0
2116	60-64	0.0
2116	65-69	0.0
2116	70-74	0.0
2116	75-79	0.0
2116	80-84	0.0
2116	85-89	0.0
2116	90-94	0.0
2116	95-99	0.0
2116	100-104	0.0
2116	105-109	0.0
2116	110-114	0.0
2116	115-119	0.0
2116	120-124	0.0
2116	125-129	0.0
2116	130-134	0.0
2116	135-139	0.0
2116	140-144	0.0
2116	145-149	0.0
2116	150-154	0.0
2116	155-159	0.0
2116	160-164	0.0
2116	165-169	0.0
2116	170-174	0.0
2116	175-179	0.0
2116	180-184	0.0
2116	185-189	0.0
2116	190-194	0.0
2116	195-199	0.0
2116	200-204	0.0
2116	205-209	0.0
2116	210-214	0.0
2116	215-219	0.0
2116	220-224	0.0
2116	225-229	0.0
2116	230-234	0.0
2116	235-239	0.0
2116	240-244	0.0
2116	245-249	0.0
2116	250-254	0.0
2116	255-259	0.0
2116	260-264	0.0
2116	265-269	0.0
2116	270-274	0.0
2116	275-279	0.0
2116	280-284	0.0
2116	285-289	0.0
2116	290-294	0.0
2116	295-299	0.0
2116	300-301	0.0
1117	1	-1.0743330291355022
1117	2	-1.9466062461618385
1117	3	8.32088801495228
1117	4	8.067874986650661
1117	5	-0.2697306491723275
1117	6	6.826929050272849
1117	7	9.05973922692699
1117	8	7.143267026228994
1117	9	4.836311330916036
1117	10-14	2.934367492002661
1117	15-19	3.6526178113855003
1117	20-24	1.2273837837048518
1117	25-29	0.5318756953579538
1117	30-34	-0.06670262490328582
1117	35-39	0.14204071933924567
1117	40-44	0.42015474052711
1117	45-49	0.9058609202857966
1117	50-54	2.3858085610318547
1117	55-59	0.0
1117	60-64	0.0
1117	65-69	0.0
1117	70-74	0.0
1117	75-79	0.0
1117	80-84	0.0
1117	85-89	0.0
1117	90-94	0.0
1117	95-99	0.0
1117	100-104	0.0
1117	105-109	0.0
1117	110-114	0.0
1117	115-119	0.0
1117	120-124	0.0
1117	125-129	0.0
1117	130-134	0.0
1117	135-139	0.0
1117	140-144	0.0
1117	145-149	0.0
1117	150-154	0.0
1117	155-159	0.0
1117	160-164	0.0
1117	165-169	0.0
1117	170-174	0.0
1117	175-179	0.0
1117	180-184	0.0
1117	185-189	0.0
1117	190-194	0.0
1117	195-199	0.0
1117	200-204	0.0
1117	205-209	0.0
1117	210-214	0.0
1117	215-219	0.0
1117	220-224	0.0
1117	225-229	0.0
1117	230-234	0.0
1117	235-239	0.0
1117	240-244	0.0
1117	245-249	0.0
1117	250-254	0.0
1117	255-259	0.0
1117	260-264	0.0
1117	265-269	0.0
1117	270-274	0.0
1117	275-279	0.0
1117	280-284	0.0
1117	285-289	0.0
1117	290-294	0.0
1117	295-299	0.0
1117	300-301	0.0
2117	1	-1.447664088332143
2117	2	-4.345836419518758
2117	3	5.663762910863815
2117	4	4.977453699861915
2117	5	-2.6544885718992006
2117	6	3.71774948758366
2117	7	9.025090173504601
2117	8	6.415894721372172
2117	9	5.022003723854045
2117	10-14	3.1874185859001436
2117	15-19	3.5620230646239683
2117	20-24	0.8045254009387754
2117	25-29	0.4848945508724576
2117	30-34	-0.8363778869955354
2117	35-39	0.22233852101081553
2117	40-44	0.25212157290844317
2117	45-49	0.07242717738244409
2117	50-54	2.2246899697641993
2117	55-59	0.0
2117	60-64	0.0
2117	65-69	0.0
2117	70-74	0.0
2117	75-79	0.0
2117	80-84	0.0
2117	85-89	0.0
2117	90-94	0.0
2117	95-99	0.0
2117	100-104	0.0
2117	105-109	0.0
2117	110-114	0.0
2117	115-119	0.0
2117	120-124	0.0
2117	125-129	0.0
2117	130-134	0.0
2117	135-139	0.0
2117	140-144	0.0
2117	145-149	0.0
2117	150-154	0.0
2117	155-159	0.0
2117	160-164	0.0
2117	165-169	0.0
2117	170-174	0.0
2117	175-179	0.0
2117	180-184	0.0
2117	185-189	0.0
2117	190-194	0.0
2117	195-199	0.0
2117	200-204	0.0
2117	205-209	0.0
2117	210-214	0.0
2117	215-219	0.0
2117	220-224	0.0
2117	225-229	0.0
2117	230-234	0.0
2117	235-239	0.0
2117	240-244	0.0
2117	245-249	0.0
2117	250-254	0.0
2117	255-259	0.0
2117	260-264	0.0
2117	265-269	0.0
2117	270-274	0.0
2117	275-279	0.0
2117	280-284	0.0
2117	285-289	0.0
2117	290-294	0.0
2117	295-299	0.0
2117	300-301	0.0
1116	1	-2.014306378838725
1116	2	-4.926338128025584
1116	3	5.528329927286174
1116	4	5.234784878088423
1116	5	-5.223673510619953
1116	6	1.940277217583958
1116	7	8.511177807606146
1116	8	6.151821701450114
1116	9	3.0684661618910276
1116	10-14	2.9297665257385113
1116	15-19	3.2387183051709165
1116	20-24	-0.93802243822919
1116	25-29	-0.9895119537497372
1116	30-34	-1.3286779953240853
1116	35-39	-0.36751205587567526
1116	40-44	0.1185218483995385
1116	45-49	0.5219678437093442
1116	50-54	2.074240518151832
1116	55-59	0.0
1116	60-64	0.0
1116	65-69	0.0
1116	70-74	0.0
1116	75-79	0.0
1116	80-84	0.0
1116	85-89	0.0
1116	90-94	0.0
1116	95-99	0.0
1116	100-104	0.0
1116	105-109	0.0
1116	110-114	0.0
1116	115-119	0.0
1116	120-124	0.0
1116	125-129	0.0
1116	130-134	0.0
1116	135-139	0.0
1116	140-144	0.0
1116	145-149	0.0
1116	150-154	0.0
1116	155-159	0.0
1116	160-164	0.0
1116	165-169	0.0
1116	170-174	0.0
1116	175-179	0.0
1116	180-184	0.0
1116	185-189	0.0
1116	190-194	0.0
1116	195-199	0.0
1116	200-204	0.0
1116	205-209	0.0
1116	210-214	0.0
1116	215-219	0.0
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2104	230-234	0.0
2104	235-239	0.0
2104	240-244	0.0
2104	245-249	0.0
2104	250-254	0.0
2104	255-259	0.0
2104	260-264	0.0
2104	265-269	0.0
2104	270-274	0.0
2104	275-279	0.0
2104	280-284	0.0
2104	285-289	0.0
2104	290-294	0.0
2104	295-299	0.0
2104	300-301	0.0
2111	1	1.2731162283543895
2111	2	2.645441072578116
2111	3	-12.48971567888214
2111	4	-12.903327806696762
2111	5	-1.3448197047337302
2111	6	-13.187883041981124
2111	7	-14.067229439800936
2111	8	-18.456916746627755
2111	9	-2.3862919981005
2111	10-14	-2.6082458333321945
2111	15-19	0.7221236259060468
2111	20-24	1.361601314069187
2111	25-29	0.5927074371017937
2111	30-34	0.16209568157952958
2111	35-39	1.9724452283295975
2111	40-44	0.5266999420394853
2111	45-49	-0.408915391311929
2111	50-54	-3.1131857432450154
2111	55-59	0.0
2111	60-64	0.0
2111	65-69	0.0
2111	70-74	0.0
2111	75-79	0.0
2111	80-84	0.0
2111	85-89	0.0
2111	90-94	0.0
2111	95-99	0.0
2111	100-104	0.0
2111	105-109	0.0
2111	110-114	0.0
2111	115-119	0.0
2111	120-124	0.0
2111	125-129	0.0
2111	130-134	0.0
2111	135-139	0.0
2111	140-144	0.0
2111	145-149	0.0
2111	150-154	0.0
2111	155-159	0.0
2111	160-164	0.0
2111	165-169	0.0
2111	170-174	0.0
2111	175-179	0.0
2111	180-184	0.0
2111	185-189	0.0
2111	190-194	0.0
2111	195-199	0.0
2111	200-204	0.0
2111	205-209	0.0
2111	210-214	0.0
2111	215-219	0.0
2111	220-224	0.0
2111	225-229	0.0
2111	230-234	0.0
2111	235-239	0.0
2111	240-244	0.0
2111	245-249	0.0
2111	250-254	0.0
2111	255-259	0.0
2111	260-264	0.0
2111	265-269	0.0
2111	270-274	0.0
2111	275-279	0.0
2111	280-284	0.0
2111	285-289	0.0
2111	290-294	0.0
2111	295-299	0.0
2111	300-301	0.0
2110	1	1.2929924160663262
2110	2	0.6801020251983445
2110	3	-12.48971567888214
2110	4	5.114141241732817
2110	5	0.8164314889867477
2110	6	10.32737326031404
2110	7	-14.067229439800936
2110	8	1.8815707025328336
2110	9	-4.668122149164979
2110	10-14	-5.958726086541631
2110	15-19	0.3678410575491675
2110	20-24	1.2821372836549259
2110	25-29	1.6168725908551274
2110	30-34	0.20793323858817558
2110	35-39	1.540657353412957
2110	40-44	0.6566671218854196
2110	45-49	-0.12688366457178546
2110	50-54	-4.7066621200845375
2110	55-59	0.0
2110	60-64	0.0
2110	65-69	0.0
2110	70-74	0.0
2110	75-79	0.0
2110	80-84	0.0
2110	85-89	0.0
2110	90-94	0.0
2110	95-99	0.0
2110	100-104	0.0
2110	105-109	0.0
2110	110-114	0.0
2110	115-119	0.0
2110	120-124	0.0
2110	125-129	0.0
2110	130-134	0.0
2110	135-139	0.0
2110	140-144	0.0
2110	145-149	0.0
2110	150-154	0.0
2110	155-159	0.0
2110	160-164	0.0
2110	165-169	0.0
2110	170-174	0.0
2110	175-179	0.0
2110	180-184	0.0
2110	185-189	0.0
2110	190-194	0.0
2110	195-199	0.0
2110	200-204	0.0
2110	205-209	0.0
2110	210-214	0.0
2110	215-219	0.0
2110	220-224	0.0
2110	225-229	0.0
2110	230-234	0.0
2110	235-239	0.0
2110	240-244	0.0
2110	245-249	0.0
2110	250-254	0.0
2110	255-259	0.0
2110	260-264	0.0
2110	265-269	0.0
2110	270-274	0.0
2110	275-279	0.0
2110	280-284	0.0
2110	285-289	0.0
2110	290-294	0.0
2110	295-299	0.0
2110	300-301	0.0
2109	1	1.5604078986729988
2109	2	2.017075230996234
2109	3	-12.48971567888214
2109	4	-12.903327806696762
2109	5	-1.246207207613363
2109	6	-13.187883041981124
2109	7	-14.067229439800936
2109	8	1.767180542561146
2109	9	-5.485570211846692
2109	10-14	-0.7771026383757409
2109	15-19	1.0626726746948485
2109	20-24	1.504240739685514
2109	25-29	1.1889446419501049
2109	30-34	0.5304391471721175
2109	35-39	1.9250950584801494
2109	40-44	0.34854195084860606
2109	45-49	-0.9433574386993939
2109	50-54	-4.125594110616179
2109	55-59	0.0
2109	60-64	0.0
2109	65-69	0.0
2109	70-74	0.0
2109	75-79	0.0
2109	80-84	0.0
2109	85-89	0.0
2109	90-94	0.0
2109	95-99	0.0
2109	100-104	0.0
2109	105-109	0.0
2109	110-114	0.0
2109	115-119	0.0
2109	120-124	0.0
2109	125-129	0.0
2109	130-134	0.0
2109	135-139	0.0
2109	140-144	0.0
2109	145-149	0.0
2109	150-154	0.0
2109	155-159	0.0
2109	160-164	0.0
2109	165-169	0.0
2109	170-174	0.0
2109	175-179	0.0
2109	180-184	0.0
2109	185-189	0.0
2109	190-194	0.0
2109	195-199	0.0
2109	200-204	0.0
2109	205-209	0.0
2109	210-214	0.0
2109	215-219	0.0
2109	220-224	0.0
2109	225-229	0.0
2109	230-234	0.0
2109	235-239	0.0
2109	240-244	0.0
2109	245-249	0.0
2109	250-254	0.0
2109	255-259	0.0
2109	260-264	0.0
2109	265-269	0.0
2109	270-274	0.0
2109	275-279	0.0
2109	280-284	0.0
2109	285-289	0.0
2109	290-294	0.0
2109	295-299	0.0
2109	300-301	0.0
2108	1	0.8004975696758443
2108	2	-0.1920404602093555
2108	3	9.44589724517735
2108	4	7.600000084801419
2108	5	1.6527801231106238
2108	6	-13.187883041981124
2108	7	-14.067229439800936
2108	8	1.5519456706234607
2108	9	-3.522654682962788
2108	10-14	-7.862286364528892
2108	15-19	-0.9932216591156475
2108	20-24	1.4153744173851344
2108	25-29	0.8815953184471148
2108	30-34	0.3741627855168872
2108	35-39	2.267143833324493
2108	40-44	0.4927815565119573
2108	45-49	-0.3315767712462261
2108	50-54	-3.7043834092693224
2108	55-59	0.0
2108	60-64	0.0
2108	65-69	0.0
2108	70-74	0.0
2108	75-79	0.0
2108	80-84	0.0
2108	85-89	0.0
2108	90-94	0.0
2108	95-99	0.0
2108	100-104	0.0
2108	105-109	0.0
2108	110-114	0.0
2108	115-119	0.0
2108	120-124	0.0
2108	125-129	0.0
2108	130-134	0.0
2108	135-139	0.0
2108	140-144	0.0
2108	145-149	0.0
2108	150-154	0.0
2108	155-159	0.0
2108	160-164	0.0
2108	165-169	0.0
2108	170-174	0.0
2108	175-179	0.0
2108	180-184	0.0
2108	185-189	0.0
2108	190-194	0.0
2108	195-199	0.0
2108	200-204	0.0
2108	205-209	0.0
2108	210-214	0.0
2108	215-219	0.0
2108	220-224	0.0
2108	225-229	0.0
2108	230-234	0.0
2108	235-239	0.0
2108	240-244	0.0
2108	245-249	0.0
2108	250-254	0.0
2108	255-259	0.0
2108	260-264	0.0
2108	265-269	0.0
2108	270-274	0.0
2108	275-279	0.0
2108	280-284	0.0
2108	285-289	0.0
2108	290-294	0.0
2108	295-299	0.0
2108	300-301	0.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.0
3	102532.0
4	4682877.0
5	7215686.0
6	6081801.0
7	2607979.0
8	18947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.142476950291084	11.766348752856496	72.30147882602087	10.789695470831546
2	85.20216639235238	5.905323570622674	4.579817248067125	4.312692788957819
3	4.6804828621749905	70.69528025140855	4.159192626800343	20.465044259616118
4	5.7751502659551255	74.99240122791603	3.728957472755793	15.503491033373049
5	24.443778415864703	62.14498608438064	4.874126875643837	8.53710862411082
6	3.672157028982701	82.43819224679783	6.5754026402215935	7.314248083997881
7	6.357160487163728	8.013625607692369	5.125463389138598	80.5037505160053
8	9.197420449334382	80.00647844633612	4.017974694958896	6.778126409370605
9	7.936702691119218	6.906143374868215	80.24944395948937	4.9077099745232005
10-14	22.984796184370875	8.152825069319135	45.36029491034054	23.502083835969447
15-19	21.43417716355513	50.872483015377554	7.932410463922625	19.76092935714469
20-24	24.642931887419746	6.04472447467052	43.70140182928676	25.610941808622982
25-29	20.117284449611613	16.599750016714033	27.032814243280157	36.25015129039419
30-34	31.845938915804673	13.952382728855737	44.73943083373127	9.462247521608322
35-39	21.109819728695637	14.212785276684897	35.60091497053819	29.07648002408128
40-44	14.633478483469267	21.81965937803132	41.64668444019869	21.900177698300723
45-49	18.34302197285906	44.03151026599843	24.388053166270574	13.237414594871941
50-54	3.8491320062442886	37.24282286853321	11.001425515205394	47.90661961001711
55-59	NaN	NaN	NaN	NaN
60-64	NaN	NaN	NaN	NaN
65-69	NaN	NaN	NaN	NaN
70-74	NaN	NaN	NaN	NaN
75-79	NaN	NaN	NaN	NaN
80-84	NaN	NaN	NaN	NaN
85-89	NaN	NaN	NaN	NaN
90-94	NaN	NaN	NaN	NaN
95-99	NaN	NaN	NaN	NaN
100-104	NaN	NaN	NaN	NaN
105-109	NaN	NaN	NaN	NaN
110-114	NaN	NaN	NaN	NaN
115-119	NaN	NaN	NaN	NaN
120-124	NaN	NaN	NaN	NaN
125-129	NaN	NaN	NaN	NaN
130-134	NaN	NaN	NaN	NaN
135-139	NaN	NaN	NaN	NaN
140-144	NaN	NaN	NaN	NaN
145-149	NaN	NaN	NaN	NaN
150-154	NaN	NaN	NaN	NaN
155-159	NaN	NaN	NaN	NaN
160-164	NaN	NaN	NaN	NaN
165-169	NaN	NaN	NaN	NaN
170-174	NaN	NaN	NaN	NaN
175-179	NaN	NaN	NaN	NaN
180-184	NaN	NaN	NaN	NaN
185-189	NaN	NaN	NaN	NaN
190-194	NaN	NaN	NaN	NaN
195-199	NaN	NaN	NaN	NaN
200-204	NaN	NaN	NaN	NaN
205-209	NaN	NaN	NaN	NaN
210-214	NaN	NaN	NaN	NaN
215-219	NaN	NaN	NaN	NaN
220-224	NaN	NaN	NaN	NaN
225-229	NaN	NaN	NaN	NaN
230-234	NaN	NaN	NaN	NaN
235-239	NaN	NaN	NaN	NaN
240-244	NaN	NaN	NaN	NaN
245-249	NaN	NaN	NaN	NaN
250-254	NaN	NaN	NaN	NaN
255-259	NaN	NaN	NaN	NaN
260-264	NaN	NaN	NaN	NaN
265-269	NaN	NaN	NaN	NaN
270-274	NaN	NaN	NaN	NaN
275-279	NaN	NaN	NaN	NaN
280-284	NaN	NaN	NaN	NaN
285-289	NaN	NaN	NaN	NaN
290-294	NaN	NaN	NaN	NaN
295-299	NaN	NaN	NaN	NaN
300-301	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.5
1	9.5
2	109.25
3	4459.0
4	81408.25
5	519693.25
6	1417904.5
7	1985258.5
8	1627018.5
9	945429.25
10	268416.25
11	48559.0
12	10515.0
13	3201.5
14	1028.5
15	677.5
16	546.25
17	389.75
18	175.5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.208665037842188E-5
2	4.828626492848346E-6
3	39.12834020841221
4	36.37574304715207
5	4.828626492848346E-6
6	36.530471554488905
7	41.759970618773515
8	20.964452472626157
9	4.828626492848346E-6
10-14	4.83056856642377
15-19	5.656320674493451
20-24	1.057868046482097
25-29	0.5329152257844019
30-34	5.794351791418015E-6
35-39	2.5728061509748295
40-44	9.657252985696691E-6
45-49	4.828626492848346E-6
50-54	44.28932106276331
55-59	100.0
60-64	100.0
65-69	100.0
70-74	100.0
75-79	100.0
80-84	100.0
85-89	100.0
90-94	100.0
95-99	100.0
100-104	100.0
105-109	100.0
110-114	100.0
115-119	100.0
120-124	100.0
125-129	100.0
130-134	100.0
135-139	100.0
140-144	100.0
145-149	100.0
150-154	100.0
155-159	100.0
160-164	100.0
165-169	100.0
170-174	100.0
175-179	100.0
180-184	100.0
185-189	100.0
190-194	100.0
195-199	100.0
200-204	100.0
205-209	100.0
210-214	100.0
215-219	100.0
220-224	100.0
225-229	100.0
230-234	100.0
235-239	100.0
240-244	100.0
245-249	100.0
250-254	100.0
255-259	100.0
260-264	100.0
265-269	100.0
270-274	100.0
275-279	100.0
280-284	100.0
285-289	100.0
290-294	100.0
295-299	100.0
300-301	100.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.0709823E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.71196632595243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.7224800493949	71.71625219810488
2	3.001310542337044	4.544702454302966
3	0.9026456983727116	2.050232421583817
4	0.36371373172050814	1.1014992723323842
5	0.23220651516794663	0.8790405928531168
6	0.1574175055652637	0.7151033328283605
7	0.12168313343639613	0.6449008510820959
8	0.06970589952833085	0.4222056574247367
9	0.04475887286257267	0.3049904047462819
>10	0.3249753831821721	4.6602051659778665
>50	0.036792219529636364	1.9737461864166368
>100	0.01953200135808878	2.6829747726838526
>500	0.0014504389304750788	0.7754024488058096
>1k	0.0010068122407678006	1.446998344792628
>5k	1.7141964192623045E-4	0.9724996950875999
>10k+	1.4977673128304428E-4	5.109246200977088
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	175207	0.8460091619324801	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	148609	0.7175773544756998	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC	110592	0.5340074610970842	No Hit
TGAAAACATCCTTGATAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	108667	0.5247123550983511	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC	54562	0.26345951870279144	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGATGTTCGTCTTCCATAAATC	47777	0.23069728794881542	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC	42709	0.20622580888306	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTCCAACAAATC	38411	0.1854723722167978	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTATGGCGTCCTTCTGCGATC	34139	0.16484447983934966	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC	29211	0.14104900848259302	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA	27244	0.1315511001711603	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC	25147	0.12142547041565735	No Hit
TGAAAACATCCTGGACAGATGCCTTCGCAGTCGTTCGTCGTGCACAAATC	24570	0.11863935292928385	No Hit
TGAAGACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGGAAATC	24019	0.11597877973172441	No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC	22616	0.10920421676225817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.939210586203465E-4	0.0	0.0
2	6.035783116060432E-4	0.0	0.0
3	6.084069380988916E-4	0.0	0.0
4	6.084069380988916E-4	0.0	0.0
5	6.180641910845882E-4	0.0	0.0
6	6.228928175774365E-4	0.0	0.0
7	6.325500705631333E-4	0.0	0.0
8	6.518645765345267E-4	0.0	0.0
9	6.663504560130717E-4	0.0	0.0
10-14	8.942616264755135E-4	0.0	0.0
15-19	0.0016629789641369702	0.0	0.0
20-24	0.0018300494407895228	0.0	1.9314505971393382E-5
25-29	0.0019797368620678214	0.0	3.090320955422941E-5
30-34	0.0022443455938759107	3.862901194278676E-6	3.669756134564743E-5
35-39	0.0024867426438168984	4.828626492848346E-6	9.946970575267591E-5
40-44	0.0026982364842036556	4.828626492848346E-6	1.0140115634981527E-4
45-49	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
50-54	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
55-59	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
60-64	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
65-69	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
70-74	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
75-79	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
80-84	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
85-89	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
90-94	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
95-99	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
100-104	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
105-109	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
110-114	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
115-119	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
120-124	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
125-129	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
130-134	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
135-139	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
140-144	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
145-149	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
150-154	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
155-159	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
160-164	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
165-169	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
170-174	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
175-179	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
180-184	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
185-189	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
190-194	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
195-199	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
200-204	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
205-209	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
210-214	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
215-219	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
220-224	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
225-229	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
230-234	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
235-239	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
240-244	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
245-249	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
250-254	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
255-259	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
260-264	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
265-269	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
270-274	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
275-279	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
280-284	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
285-289	0.0027088594624879218	4.828626492848346E-6	1.0140115634981527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACATTC	107640	0.0	71.88697	5
AAGACAT	120980	0.0	71.86621	3
ACATTCT	229680	0.0	71.83293	6
TGAAAAC	301760	0.0	71.49626	1
AACATCT	81175	0.0	71.463875	5
ACATCTT	100200	0.0	71.443146	6
ACATCCT	263185	0.0	71.320724	6
AACATTC	110100	0.0	71.317314	5
CGAAGAC	49005	0.0	71.298454	1
AGACATT	103930	0.0	71.162384	4
CATCCTT	264950	0.0	71.12592	7
TGAAGAC	66125	0.0	71.12229	1
GAAGACA	130920	0.0	71.10871	2
AAACATC	239035	0.0	70.99958	4
AACGTCC	22735	0.0	70.99308	5
AACATCC	203545	0.0	70.93554	5
AAAACAT	282500	0.0	70.84838	3
ACAACAT	61900	0.0	70.527695	3
GACAACA	61750	0.0	70.51713	2
CATTCTT	250590	0.0	70.50303	7
>>END_MODULE