FastQCFastQC Report
Wed 5 Aug 2015
000000000-AFJR0 l01n03.3330000000edf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJR0 l01n03.3330000000edf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20709823
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC1752070.8460091619324801No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC1486090.7175773544756998No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC1105920.5340074610970842No Hit
TGAAAACATCCTTGATAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC1086670.5247123550983511No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC545620.26345951870279144No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGATGTTCGTCTTCCATAAATC477770.23069728794881542No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC427090.20622580888306No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTCCAACAAATC384110.1854723722167978No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTATGGCGTCCTTCTGCGATC341390.16484447983934966No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC292110.14104900848259302No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA272440.1315511001711603No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC251470.12142547041565735No Hit
TGAAAACATCCTGGACAGATGCCTTCGCAGTCGTTCGTCGTGCACAAATC245700.11863935292928385No Hit
TGAAGACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGGAAATC240190.11597877973172441No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC226160.10920421676225817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATTC1076400.071.886975
AAGACAT1209800.071.866213
ACATTCT2296800.071.832936
TGAAAAC3017600.071.496261
AACATCT811750.071.4638755
ACATCTT1002000.071.4431466
ACATCCT2631850.071.3207246
AACATTC1101000.071.3173145
CGAAGAC490050.071.2984541
AGACATT1039300.071.1623844
CATCCTT2649500.071.125927
TGAAGAC661250.071.122291
GAAGACA1309200.071.108712
AAACATC2390350.070.999584
AACGTCC227350.070.993085
AACATCC2035450.070.935545
AAAACAT2825000.070.848383
ACAACAT619000.070.5276953
GACAACA617500.070.517132
CATTCTT2505900.070.503037
Produced by FastQC (version 0.11.2)