FastQCFastQC Report
Wed 5 Aug 2015
000000000-AFJR0 l01n02.3320000000edf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJR0 l01n02.3320000000edf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20709823
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAGTCTCGT10120414.886767984448732No Hit
GTATGCGCTGTA8672484.187616668669742No Hit
TCCGACACAATT8648824.176192138387663No Hit
TGAGTCACTGGT8276783.996547918347733No Hit
ACACCTGGTGAT8183453.9514823472899794No Hit
CAGGCGTATTGG8154973.9377304190383473No Hit
GAACCAAAGGAT8056693.8902746778666333No Hit
CTAACCTCCGCT8042783.883558058415081No Hit
TACACGATCTAC7897703.813504345256838No Hit
GGAGACAAGGGA7860713.7956432558597917No Hit
AATCCGTACAGC7804653.768573975740884No Hit
GCACACACGTTA7582063.6610935786365726No Hit
TTACTGTGCGAT7524293.6331986033873878No Hit
CCTCGTTCGACT7348163.5481520049688497No Hit
GCTGATGAGCTG7303423.526548730039846No Hit
CCAGTGTATGCA7270063.510440432059704No Hit
GACTTGGTATTC7095763.4262774722893576No Hit
GTACATACCGGT6934913.348609015151892No Hit
AGCTGTTGTTTG6646083.209143796158953No Hit
AGGCTACACGAC6143652.9665391152787737No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG7600.06.00000055
CTCAAGG953.430201E-66.00000054
AGCCACG953.430201E-66.00000053
GGATCTG953.430201E-66.00000056
GTCTAGT1900.06.00000056
CCGTCAG953.430201E-66.00000054
ATCCGTG953.430201E-66.00000052
GTCAGCA953.430201E-66.00000056
CGCTCGG953.430201E-66.00000054
TGCGCAG1601.0913936E-116.00000054
TGTCTGA806.5398744E-56.00000055
CAAGGGC3200.06.00000056
CGGCTAC7900.06.00000051
CTTTCGG953.430201E-66.00000054
GGCGCAT1950.06.00000053
ACGAGGG806.5398744E-56.00000055
AGGCTGC13100.06.00000051
TGTCCGG806.5398744E-56.00000055
ACGCGTA1900.06.00000052
CCTTCGG1101.8156061E-76.04
Produced by FastQC (version 0.11.2)