##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFJR0 l01n01.3310000000edf9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20709823
Sequences flagged as poor quality	0
Sequence length	301
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.198658095725875	34.0	34.0	34.0	32.0	34.0
2	33.6948628677319	34.0	34.0	34.0	34.0	34.0
3	33.80857393131752	34.0	34.0	34.0	34.0	34.0
4	33.62630062072476	34.0	34.0	34.0	34.0	34.0
5	33.796818978124534	34.0	34.0	34.0	34.0	34.0
6	37.37379199233137	38.0	38.0	38.0	37.0	38.0
7	37.297004807815114	38.0	38.0	38.0	37.0	38.0
8	36.91852977207966	38.0	38.0	38.0	35.0	38.0
9	36.91495953393711	38.0	38.0	38.0	36.0	38.0
10-14	36.981424515313336	38.0	37.2	38.0	35.4	38.0
15-19	36.91393067917577	38.0	37.8	38.0	35.8	38.0
20-24	36.23316167405197	38.0	37.0	38.0	33.8	38.0
25-29	36.818880624909255	38.0	37.8	38.0	36.0	38.0
30-34	36.892116489841555	38.0	38.0	38.0	36.0	38.0
35-39	37.2374128740743	38.0	38.0	38.0	36.8	38.0
40-44	37.23726609348616	38.0	38.0	38.0	36.8	38.0
45-49	36.71096933083397	38.0	37.6	38.0	34.8	38.0
50-54	36.09686562748509	38.0	37.2	38.0	31.8	38.0
55-59	35.93460176844582	38.0	37.0	38.0	31.4	38.0
60-64	34.48298020702543	38.0	35.4	38.0	24.0	38.0
65-69	34.15528736290987	38.0	34.4	38.0	22.4	38.0
70-74	34.489571156643876	38.0	35.6	38.0	23.6	38.0
75-79	34.293894583261284	38.0	34.6	38.0	22.8	38.0
80-84	33.87754451595264	38.0	34.0	38.0	21.4	38.0
85-89	33.742017853073875	38.0	34.0	38.0	21.2	38.0
90-94	33.79371136102902	38.0	34.0	38.0	21.4	38.0
95-99	33.31211336765167	37.6	33.4	38.0	19.0	38.0
100-104	33.6024402622852	37.8	34.0	38.0	21.0	38.0
105-109	33.58892402894993	38.0	34.0	38.0	20.8	38.0
110-114	33.44926484403078	38.0	34.0	38.0	19.6	38.0
115-119	33.01524804919868	37.2	32.8	38.0	17.2	38.0
120-124	33.211080857620075	37.6	33.4	38.0	19.2	38.0
125-129	33.38448594176783	38.0	33.8	38.0	19.8	38.0
130-134	33.00772230646298	37.4	32.6	38.0	17.4	38.0
135-139	33.18982094632098	38.0	32.8	38.0	19.0	38.0
140-144	33.24572036178194	37.6	33.2	38.0	19.4	38.0
145-149	33.036501992315436	37.2	32.6	38.0	17.6	38.0
150-154	33.033846122200075	37.2	32.6	38.0	18.2	38.0
155-159	32.97049271739309	37.0	32.2	38.0	17.6	38.0
160-164	32.81776742370034	37.0	31.6	38.0	14.0	38.0
165-169	32.72501140159431	37.0	31.2	38.0	12.8	38.0
170-174	32.8539649904299	37.0	31.8	38.0	16.6	38.0
175-179	32.37252397569984	37.0	30.8	38.0	11.6	38.0
180-184	32.48230897965666	37.0	30.4	38.0	15.2	38.0
185-189	32.27006669250626	37.0	30.4	38.0	12.2	38.0
190-194	31.946929937547026	37.0	29.0	38.0	11.0	38.0
195-199	31.722593099902394	37.0	28.6	38.0	11.0	38.0
200-204	31.56751291404084	37.0	27.8	38.0	11.0	38.0
205-209	31.45955642402158	37.0	27.4	38.0	11.0	38.0
210-214	31.4380279155452	37.0	26.8	38.0	11.0	38.0
215-219	30.829502067690292	36.0	25.6	38.0	11.0	38.0
220-224	30.68921427285979	35.8	25.0	38.0	11.0	38.0
225-229	30.220094445037024	35.6	24.0	38.0	11.0	38.0
230-234	30.595492380596397	36.0	25.0	38.0	11.0	38.0
235-239	30.404476117444364	35.8	24.6	38.0	11.0	38.0
240-244	30.37216988286186	35.8	24.6	38.0	11.0	38.0
245-249	30.057641883274425	35.4	23.6	38.0	10.8	38.0
250-254	29.599415610650077	34.6	23.0	37.8	10.4	38.0
255-259	28.50495886903524	33.2	21.4	37.4	10.0	37.8
260-264	27.37733374157761	31.4	19.0	37.0	9.8	37.6
265-269	27.044341064624255	31.2	18.8	36.8	9.6	37.4
270-274	26.827563499697703	30.6	18.6	36.8	9.4	37.6
275-279	26.340883096876304	28.8	17.4	36.8	9.2	37.6
280-284	25.60683267066068	27.8	16.6	36.0	8.8	37.2
285-289	26.394338696182967	28.8	17.2	36.8	9.4	37.6
290-294	24.56358551205387	25.6	15.6	35.0	8.4	37.0
295-299	23.166743511038213	23.6	14.0	33.0	8.2	37.0
300-301	18.65026164154083	18.0	10.0	25.5	7.5	33.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-8.16079915074355
1101	2	-1.6445113397774378
1101	3	-0.15974812142591333
1101	4	0.1776612784405529
1101	5	0.08386098199134295
1101	6	0.0316946733920247
1101	7	0.15812102191473798
1101	8	0.4552881081257496
1101	9	0.3793369063916785
1101	10-14	0.3731310070727005
1101	15-19	0.5018101397903578
1101	20-24	1.1770328826224628
1101	25-29	0.881625283924464
1101	30-34	0.6598523350085443
1101	35-39	0.3685418253669823
1101	40-44	0.3377900074599083
1101	45-49	0.4047836598322547
1101	50-54	0.5140533595291146
1101	55-59	0.5264162613034458
1101	60-64	0.8806137174926008
1101	65-69	0.9107474509312254
1101	70-74	0.8466079171682992
1101	75-79	0.741769852550803
1101	80-84	1.051328270968078
1101	85-89	1.1959495579017272
1101	90-94	1.0284221764289896
1101	95-99	1.3332263568686997
1101	100-104	0.9840712412798212
1101	105-109	1.0382778861359014
1101	110-114	0.9617226177902722
1101	115-119	1.3230401367778057
1101	120-124	1.2327491478990567
1101	125-129	1.0746665941279616
1101	130-134	1.140667479746135
1101	135-139	1.1209742389027753
1101	140-144	1.0954567125325525
1101	145-149	1.0218557568458877
1101	150-154	1.0913749765009229
1101	155-159	1.09575819360483
1101	160-164	1.16876104375811
1101	165-169	1.266805706896342
1101	170-174	1.1506914517310136
1101	175-179	1.2022163240893988
1101	180-184	1.2453514975113649
1101	185-189	1.1095500582951772
1101	190-194	1.4783016287834911
1101	195-199	1.3987271499543752
1101	200-204	1.3194720567802634
1101	205-209	1.4023045129701543
1101	210-214	1.3753695930853844
1101	215-219	1.375553538681661
1101	220-224	1.3104402377448672
1101	225-229	1.630422527528708
1101	230-234	1.5243142262943117
1101	235-239	1.4428036319812279
1101	240-244	1.3555356715631746
1101	245-249	1.2781252709997908
1101	250-254	1.0071101988933115
1101	255-259	1.0818514626608682
1101	260-264	0.9646708403028796
1101	265-269	1.014650526545104
1101	270-274	1.0049953122955735
1101	275-279	0.47687890781698883
1101	280-284	0.5422711066356705
1101	285-289	0.4288679706253262
1101	290-294	-0.49421868156757753
1101	295-299	0.8356464605375571
1101	300-301	1.0116234447708798
1102	1	0.46297253002621375
1102	2	0.07820007628983916
1102	3	0.03387731578367692
1102	4	0.2405081261492299
1102	5	0.08636258794901153
1102	6	0.0026959786622953175
1102	7	0.09527196685651518
1102	8	0.3623817244128631
1102	9	0.3037975258599346
1102	10-14	0.2708197230915843
1102	15-19	0.41657186186552053
1102	20-24	0.9683904786734701
1102	25-29	0.6915515956368026
1102	30-34	0.425375831835062
1102	35-39	0.2500377389898105
1102	40-44	0.04719247998480114
1102	45-49	-0.0673601531870176
1102	50-54	0.19651040022530708
1102	55-59	0.080692370572514
1102	60-64	0.5593554122546465
1102	65-69	0.5584779670542446
1102	70-74	0.5640417665358299
1102	75-79	0.5243146502321991
1102	80-84	0.6329814618283081
1102	85-89	0.7885128314741081
1102	90-94	0.6750247601333328
1102	95-99	0.8601283854502313
1102	100-104	0.7658071268914881
1102	105-109	0.7609924791489249
1102	110-114	0.5747244081315799
1102	115-119	0.953506152618651
1102	120-124	0.919710914114404
1102	125-129	0.6497249662414575
1102	130-134	0.5667018780306563
1102	135-139	0.3761184799841004
1102	140-144	0.5031947895953834
1102	145-149	0.6454953494094156
1102	150-154	0.6846888464926693
1102	155-159	0.7399787107585141
1102	160-164	0.7854398390201638
1102	165-169	0.685757009114873
1102	170-174	0.7269294505472388
1102	175-179	0.7355387785215299
1102	180-184	0.7580050179046722
1102	185-189	0.8894776947584191
1102	190-194	1.1282314463264314
1102	195-199	0.7618583841176303
1102	200-204	0.8250233928220752
1102	205-209	0.8886642506960882
1102	210-214	0.8674272225809823
1102	215-219	0.8979120428847125
1102	220-224	0.9079796455017188
1102	225-229	1.164312556009655
1102	230-234	0.8205178307153034
1102	235-239	0.6260714086251582
1102	240-244	0.6353927328863129
1102	245-249	0.6297229768710437
1102	250-254	0.7271447822949781
1102	255-259	0.8960354309506293
1102	260-264	0.9582751087915078
1102	265-269	0.8268048645618258
1102	270-274	0.30575371597723233
1102	275-279	0.6350805293079915
1102	280-284	0.5338060630521149
1102	285-289	1.5942532518889116
1102	290-294	0.3169690277190931
1102	295-299	-0.02114838331225144
1102	300-301	0.543083774349661
1103	1	0.5550404142960161
1103	2	0.12721576229835563
1103	3	0.0435472265700767
1103	4	0.22339864994871306
1103	5	0.095584546199035
1103	6	0.20647762557295124
1103	7	0.15829595279224407
1103	8	0.45836811886550777
1103	9	0.34841116383044834
1103	10-14	0.3217894398801988
1103	15-19	0.4503256295522746
1103	20-24	1.0950423136369594
1103	25-29	0.8278532998165389
1103	30-34	0.5753626800166245
1103	35-39	0.3116296191165091
1103	40-44	0.21773410325230458
1103	45-49	0.2005118056889259
1103	50-54	0.3185196957683658
1103	55-59	0.2905977090810197
1103	60-64	0.7290713253812413
1103	65-69	0.7283764649082514
1103	70-74	0.6527550559309461
1103	75-79	0.6381331824531884
1103	80-84	0.7079730613874062
1103	85-89	0.8953674015085866
1103	90-94	0.9157512959065173
1103	95-99	1.1178181344737368
1103	100-104	0.9520963112778844
1103	105-109	0.8272350895243363
1103	110-114	0.8506614320656212
1103	115-119	1.086769611779637
1103	120-124	1.0693977459487556
1103	125-129	1.021853114104502
1103	130-134	1.0382903903858463
1103	135-139	0.7210224682510074
1103	140-144	0.7874724862881379
1103	145-149	0.54224165273056
1103	150-154	0.6904238524194
1103	155-159	0.7481379917710882
1103	160-164	0.8482454373628769
1103	165-169	0.921607618264332
1103	170-174	0.8084608697423761
1103	175-179	1.0679231817750363
1103	180-184	0.9839725191469171
1103	185-189	1.0586281479129553
1103	190-194	1.2745221569313756
1103	195-199	1.0306223117434783
1103	200-204	1.031134428185112
1103	205-209	1.2994683591413363
1103	210-214	1.3063374223797162
1103	215-219	1.4794442209168643
1103	220-224	1.2393669751806193
1103	225-229	1.407410840971572
1103	230-234	1.3977114043654666
1103	235-239	1.312804646658332
1103	240-244	1.2917125677774877
1103	245-249	1.0003884313515456
1103	250-254	1.1315363761495725
1103	255-259	0.8988612993961809
1103	260-264	1.3741275504068646
1103	265-269	1.859096695804233
1103	270-274	0.6917462896280604
1103	275-279	1.3695815226385086
1103	280-284	0.9677062089726043
1103	285-289	1.739041040306816
1103	290-294	0.01721110798007075
1103	295-299	1.0958625619383824
1103	300-301	1.1683190582385876
2116	1	0.48494496681389165
2116	2	0.10276284065569996
2116	3	0.04696301771652145
2116	4	0.2133918027898929
2116	5	0.10037453244991923
2116	6	0.25305564696992633
2116	7	0.2766369917034197
2116	8	0.4392748708006806
2116	9	0.3173802772180778
2116	10-14	0.32831207193775214
2116	15-19	0.4670298753077802
2116	20-24	1.0928108964585377
2116	25-29	0.7908893344514141
2116	30-34	0.4973240523741467
2116	35-39	0.18049800536848437
2116	40-44	0.17173681361720838
2116	45-49	0.10640286933974608
2116	50-54	0.0907892297638071
2116	55-59	0.042026604021224045
2116	60-64	0.36871206311985816
2116	65-69	0.45844863447684503
2116	70-74	0.41328126959680844
2116	75-79	0.27171948171276483
2116	80-84	0.5326927557213494
2116	85-89	0.8835065948217249
2116	90-94	0.7983398871306235
2116	95-99	0.9365545163593509
2116	100-104	0.8349823112051027
2116	105-109	0.7678585666041187
2116	110-114	0.7815759364385002
2116	115-119	1.0499132246173843
2116	120-124	1.0290248030490616
2116	125-129	0.8335106784354736
2116	130-134	1.038884468049453
2116	135-139	0.6262182992162053
2116	140-144	0.8209029665167691
2116	145-149	0.3461660676273084
2116	150-154	0.4375810462968559
2116	155-159	0.024753938800074593
2116	160-164	0.9779559643842717
2116	165-169	0.9855217758579187
2116	170-174	0.4545627786097839
2116	175-179	1.0876406044843137
2116	180-184	1.1194385862808858
2116	185-189	1.2841210789005544
2116	190-194	1.2341652473528484
2116	195-199	1.1356854959021163
2116	200-204	1.183673660539725
2116	205-209	1.3247582957109927
2116	210-214	1.0646525457083236
2116	215-219	0.9942922301049357
2116	220-224	1.0899310576441188
2116	225-229	1.836069131113419
2116	230-234	0.8555378558245899
2116	235-239	1.2705708451228688
2116	240-244	1.2327843895571071
2116	245-249	1.1912771609250257
2116	250-254	1.171281723756671
2116	255-259	1.1784029000590373
2116	260-264	1.4509613717952767
2116	265-269	1.7577540083621415
2116	270-274	1.7992196086337522
2116	275-279	-2.4510776641023533
2116	280-284	1.041703988181343
2116	285-289	-1.763655507663195
2116	290-294	-0.2992922905246296
2116	295-299	0.5672525232098238
2116	300-301	1.5366862303803117
1117	1	0.5681138287241794
1117	2	0.12089808659239054
1117	3	0.06935495181345885
1117	4	0.2514556383838453
1117	5	0.08922225980462173
1117	6	0.11882327560508799
1117	7	0.19978649653128855
1117	8	0.4340453614564268
1117	9	0.35732618558221674
1117	10-14	0.32129610657852936
1117	15-19	0.3984538902189101
1117	20-24	1.0666544118015437
1117	25-29	0.7747464987682164
1117	30-34	0.526292043615392
1117	35-39	0.22634210035230495
1117	40-44	0.18096349511251475
1117	45-49	0.11688312788659516
1117	50-54	0.19503574271043078
1117	55-59	0.16601724376491944
1117	60-64	0.644567701300268
1117	65-69	0.6304967927876675
1117	70-74	0.5656223166855554
1117	75-79	0.5467989041666215
1117	80-84	0.7658821308728747
1117	85-89	0.9925687837251189
1117	90-94	0.7393945225624918
1117	95-99	0.8579338352581587
1117	100-104	0.7095037214592708
1117	105-109	0.7459981521922998
1117	110-114	0.7264779584740566
1117	115-119	1.0043026563036719
1117	120-124	0.9966047374931222
1117	125-129	0.8658329749890896
1117	130-134	0.8341241357061051
1117	135-139	0.8061435770466545
1117	140-144	0.32538336183819894
1117	145-149	0.8080805863424487
1117	150-154	0.7413467521012294
1117	155-159	0.7703084737001547
1117	160-164	0.7496712715004747
1117	165-169	0.9525929061314642
1117	170-174	0.776930748872509
1117	175-179	0.9109858007487901
1117	180-184	1.0145972838702022
1117	185-189	0.905639086322104
1117	190-194	0.3224609085545538
1117	195-199	1.0071582044220335
1117	200-204	0.6715506726231268
1117	205-209	0.9246482183507112
1117	210-214	0.8106097964459344
1117	215-219	0.4189593616534637
1117	220-224	0.26214143165744375
1117	225-229	1.3137174584391786
1117	230-234	1.1018440015221316
1117	235-239	1.1580842364376949
1117	240-244	1.1904180226412535
1117	245-249	1.1387777728830848
1117	250-254	0.8399343559255286
1117	255-259	1.0733176788599472
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2108	245-249	-0.36204981974641015
2108	250-254	-0.4342546635917124
2108	255-259	-0.11704649904723041
2108	260-264	-0.031946460297554324
2108	265-269	-0.4599196026754697
2108	270-274	0.2431189084229466
2108	275-279	0.03275621655065919
2108	280-284	-0.947950788407514
2108	285-289	-0.20930934490125708
2108	290-294	0.3527728369909262
2108	295-299	0.0370733384710249
2108	300-301	-0.5330638145326319
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	5.0
11	174.0
12	1673.0
13	2707.0
14	2410.0
15	6985.0
16	22260.0
17	35410.0
18	62168.0
19	143273.0
20	379210.0
21	615403.0
22	575301.0
23	499781.0
24	487206.0
25	508644.0
26	541322.0
27	579879.0
28	634368.0
29	705525.0
30	808789.0
31	948281.0
32	1145716.0
33	1398005.0
34	1727627.0
35	2294211.0
36	3533776.0
37	3049713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.51519291111276	92.52167437645411	2.664209153308553	2.298923559124576
2	91.0430475431876	4.115216243035974	2.8066777779800436	2.035058435796385
3	2.4084078362234194	3.2115291376464206	2.532049646199294	91.84801337993088
4	2.2439641323829758	3.34706385467418	92.2059739477252	2.2029980652176504
5	2.335910838059794	3.3352288911402095	2.5795923026478786	91.74926796815211
6	2.6346241587868713	4.661295270365179	29.259110519679478	63.44497005116847
7	4.57436550761443	63.57961630092155	5.790030170706915	26.055988020757106
8	6.5943103424882	75.7666446497394	11.030176356408262	6.608868651364137
9	7.413018450230116	16.999290626481937	73.00439506412005	2.5832958591678934
10-14	38.99137428649197	21.934524500764685	24.097336804858255	14.97676440788509
15-19	3.0973330868158557	40.65507754460287	45.06818334468624	11.179406023895037
20-24	19.24689554324052	45.37309565610484	28.74423311102176	6.6357756896328866
25-29	38.2462886162406	3.1265936278315234	51.662521226400834	6.964596529527041
30-34	26.803674121390326	13.924062698366022	39.75613696728055	19.516126212963105
35-39	3.2560878287018804	91.27298446821045	2.6147959879636242	2.856131715124044
40-44	38.270266156802315	2.9907722046090015	20.757571938570983	37.9813897000177
45-49	20.527644322582784	20.79885959425436	54.432453354741526	4.241042728421326
50-54	29.755542080467904	30.09285449194107	29.599271302283526	10.5523321253075
55-59	36.595595075611946	7.353578413179989	36.498786904107625	19.55203960710044
60-64	30.29852791588455	21.45790533593191	27.91159093496796	20.331975813215582
65-69	27.93645932833223	20.87866230815504	29.69431412785682	21.490564235655913
70-74	29.563956657860217	9.763376044468174	37.69133119541056	22.981336102261043
75-79	19.024535682142236	9.747899518361859	32.719134577190175	38.508430222305726
80-84	35.933158747568925	14.827778283013101	29.29601604386603	19.94304692555195
85-89	31.825425636202958	19.65004141513143	28.816693837349256	19.70783911131636
90-94	32.52685030320396	17.644780336595844	28.850512573212843	20.977856786987353
95-99	23.008516442101723	20.584619220773604	28.757385746724427	27.649478590400246
100-104	25.7449571512493	14.180637573804352	32.00738470856968	28.067020566376673
105-109	22.905048849816755	18.13180932343899	32.80050241668424	26.162639410060013
110-114	20.002543913728896	24.800690462725274	31.776567966412923	23.420197657132906
115-119	31.761380617217682	12.511962078050562	27.179806582011373	28.54685072272038
120-124	24.43270734050732	15.967107781032594	35.76408044453496	23.836104433925122
125-129	26.68877868739129	17.134878816482697	38.99208517558727	17.184257320538745
130-134	25.783088846754502	22.02240258839125	28.263492685296576	23.931015879557666
135-139	26.21298556996758	22.385688514229514	31.176161061897695	20.225164853905213
140-144	33.14421951648718	20.02272235937991	32.46933392162749	14.363724202505429
145-149	32.09426066894291	20.14020768078867	29.293755563664643	18.471776086603782
150-154	24.8195136552987	23.971293890817485	30.19657687442368	21.012615579460135
155-159	19.325762773628274	28.356559496222772	31.31473287605398	21.00294485409497
160-164	15.144335480791113	33.74100110531133	36.93653521257827	14.178128201319284
165-169	17.739368990785877	37.84406984643595	31.18807225626844	13.228488906509728
170-174	15.685599600305574	38.942665897498586	33.01767697557598	12.354057526619858
175-179	25.42717509454548	27.62651514100923	25.74891132814007	21.197398436305217
180-184	28.239436193302954	34.94296382488359	23.137782347075188	13.67981763473827
185-189	25.969910604249062	30.15366477576025	23.889452255526496	19.986972364464183
190-194	24.165995712874707	23.203055500970613	30.64932823950545	21.98162054664923
195-199	23.305130224880937	25.310135463651417	31.410127287820334	19.974607023647312
200-204	19.748019842875323	27.504216212161214	28.500451756676682	24.24731218828678
205-209	21.886857025237312	30.376847190000394	34.69895592629302	13.037339858469277
210-214	24.29537660241963	32.757483215041475	33.70710123638051	9.240038946158387
215-219	19.130089649155547	44.49941031360126	25.75839210279235	10.612107934450849
220-224	25.855491926164078	39.64795949423301	24.257866366727818	10.238682212875096
225-229	27.94760128539981	34.10996840581094	24.70995089720959	13.232479411579657
230-234	22.87787571166596	26.474112422814038	34.39836328091173	16.249648584608266
235-239	19.85833909839185	20.02750907327951	44.584374401370994	15.529777426957653
240-244	22.956996103442904	14.051327628568108	46.86466773421171	16.127008533777285
245-249	29.20694634652099	18.12537934898716	43.57018230287059	9.09749200162126
250-254	24.94599277475186	19.673882656719748	45.46058474979813	9.919539818730259
255-259	28.037331389418274	24.446174359052407	38.2224014565611	9.294092794968217
260-264	20.290833030377232	32.492207441097634	37.48417912448399	9.732780404041156
265-269	27.60350813251799	31.796112974800074	28.716274818779226	11.88410407390271
270-274	27.102901484164388	34.70352217130135	28.711570082172315	9.48200626236195
275-279	38.63511616058874	30.753471988000282	22.044829841841704	8.566582009569276
280-284	29.19034830498345	32.29612400230549	23.556505417279936	14.957022275431115
285-289	38.40099230985167	23.865379892987587	25.003816546637946	12.729811250522797
290-294	30.063566459603873	27.583179980164967	27.811560515177796	14.541693045053359
295-299	30.535410685808888	23.878477564896503	29.24960533219455	16.33650641710006
300-301	31.660400074901656	21.025429383217297	31.10536633294096	16.208804208940087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.25
6	0.0
7	0.0
8	0.0
9	0.0
10	0.25
11	0.25
12	0.25
13	0.25
14	1.5
15	1.5
16	1.75
17	5.75
18	9.75
19	26.75
20	103.25
21	169.75
22	207.0
23	299.25
24	423.25
25	889.5
26	646.0
27	955.5
28	1219.0
29	2099.25
30	5186.5
31	6021.5
32	7065.75
33	11349.5
34	29308.5
35	67927.75
36	59834.0
37	119068.75
38	399322.25
39	1081602.25
40	597012.25
41	482656.75
42	422711.5
43	510485.25
44	458083.0
45	368267.75
46	378630.5
47	405136.0
48	344125.25
49	511114.75
50	180323.5
51	91368.0
52	60065.25
53	38486.25
54	27613.75
55	23161.75
56	15121.75
57	10159.0
58	7802.0
59	5058.0
60	3294.0
61	2659.0
62	2280.75
63	1311.25
64	939.0
65	595.5
66	352.0
67	236.0
68	198.75
69	164.25
70	123.75
71	93.0
72	56.0
73	47.25
74	35.5
75	27.0
76	23.0
77	24.0
78	23.25
79	19.0
80	18.0
81	18.0
82	17.0
83	12.5
84	15.5
85	16.0
86	11.25
87	8.5
88	12.25
89	8.75
90	8.0
91	6.75
92	4.0
93	8.25
94	8.25
95	7.0
96	4.25
97	2.25
98	3.75
99	22.0
100	48.000000000000064
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	9.65725298569669E-7
30-34	4.828626492848346E-6
35-39	3.862901194278676E-6
40-44	4.828626492848346E-6
45-49	4.828626492848346E-6
50-54	4.828626492848346E-6
55-59	4.828626492848346E-6
60-64	4.828626492848346E-6
65-69	4.828626492848346E-6
70-74	4.828626492848346E-6
75-79	6.7600770899876846E-6
80-84	7.725802388557352E-6
85-89	4.828626492848346E-6
90-94	4.828626492848346E-6
95-99	4.828626492848346E-6
100-104	4.828626492848346E-6
105-109	5.794351791418015E-6
110-114	5.794351791418015E-6
115-119	7.725802388557352E-6
120-124	4.828626492848346E-6
125-129	7.725802388557352E-6
130-134	8.691527687127022E-6
135-139	5.794351791418015E-6
140-144	8.691527687127022E-6
145-149	8.691527687127022E-6
150-154	6.7600770899876846E-6
155-159	1.158870358283603E-5
160-164	8.691527687127022E-6
165-169	8.691527687127022E-6
170-174	9.657252985696691E-6
175-179	6.7600770899876846E-6
180-184	7.725802388557352E-6
185-189	9.657252985696691E-6
190-194	6.7600770899876846E-6
195-199	8.691527687127022E-6
200-204	8.691527687127022E-6
205-209	7.725802388557352E-6
210-214	5.794351791418015E-6
215-219	7.725802388557352E-6
220-224	7.725802388557352E-6
225-229	9.657252985696691E-6
230-234	7.725802388557352E-6
235-239	6.7600770899876846E-6
240-244	9.657252985696691E-6
245-249	4.828626492848346E-6
250-254	6.7600770899876846E-6
255-259	8.691527687127022E-6
260-264	9.657252985696691E-6
265-269	4.828626492848346E-6
270-274	5.794351791418015E-6
275-279	9.657252985696691E-6
280-284	7.725802388557352E-6
285-289	6.7600770899876846E-6
290-294	9.657252985696691E-6
295-299	4.828626492848346E-6
300-301	4.828626492848346E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.0709823E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.447708976129554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2050125398813	3.5672855420421348
2	10.77376639898333	0.958371550389623
3	3.653787706585707	0.48752953138359206
4	1.7845477504370635	0.31748596191802925
5	0.9168605754038399	0.20389645055414832
6	0.5205233259683656	0.13890817615165857
7	0.27170250906688215	0.08459175818895853
8	0.19592434670163897	0.06971315803737589
9	0.09579248789738905	0.03834513974402991
>10	0.7888991323241341	0.6906272717255423
>50	0.2160601046936109	0.7323761155075511
>100	0.5038069246893702	3.9483438500833308
>500	0.027082998351031447	0.8544517617796076
>1k	0.03230649715029763	3.212337567359173
>5k	0.004076107865949112	1.2440404523207296
>10k+	0.009850593999868758	83.45169571281446
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	5238942	25.296894135695897	No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	2126992	10.270449921276487	No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG	1514355	7.312254672577357	No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	757212	3.6562939239026813	No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	670482	3.237507148177944	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG	567452	2.7400137606197794	No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG	454348	2.19387678977266	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	414744	2.0026438661498944	No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	346322	1.6722595842562245	No Hit
AGCTCTCAAAGTGTATATCCATATTGCTGCGGTTAAAAAGCTCGTAGTTG	288127	1.3912576655049151	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	258600	1.248682811050582	No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	246659	1.19102418209948	No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG	220087	1.062717918931514	No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	181059	0.8742662841686286	No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	157551	0.7607549325747497	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG	155673	0.7516867720211805	No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	154648	0.746737429866011	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	145885	0.7044241759091809	No Hit
AGCTCCACTAGTATAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	135713	0.6553073872239276	No Hit
AGCTCTCATACTGCATTGAATTGTTGTTGCGGTTAAAAAGCTCGTAGTTG	133006	0.642236295307787	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	128489	0.620425389439591	No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	122143	0.5897829257159755	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	114827	0.5544566942942969	No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	89860	0.43390037664735226	No Hit
AGCTCCACTAGTGTAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	84167	0.4064110060235667	No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	82235	0.39708209963938373	No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG	80882	0.3905489679945599	No Hit
AGCTCCATTAATATAGATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	73361	0.35423286814184746	No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	63588	0.3070427014272406	No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	60728	0.2932328296576943	No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	59905	0.28925887005408013	No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG	56098	0.27087628899580646	No Hit
AGCTCCAATAGCGTATACTAATTTTGTTGCAGTTAAAAAGCTCGTAGTTG	55687	0.26889172350724583	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	51997	0.25107409174863543	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	50776	0.2451783388008676	No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	47146	0.22765042463182808	No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG	46639	0.225202310999954	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	46424	0.22416415630399159	No Hit
AGCTCTAATAGTGTATATAAATACTGTTGCGGTTAAAAAGCTCGTAGTTG	44557	0.21514911064184372	No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	41804	0.20185590190703223	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG	41058	0.19825374654336736	No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	40863	0.19731216437726193	No Hit
AGCTCCAATAGCGTATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	39963	0.19296640053369843	No Hit
AGCTCCAGTAGCGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG	39777	0.19206827600602863	No Hit
AGCTCTTCTAGCATATATTAAAGTTGCTGCAGTTAAAACGCTCGTAGTTT	38834	0.18751488122327264	No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	38089	0.18391755448610064	No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	37386	0.18052303006162823	No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG	31100	0.15017028392758355	No Hit
AGCTCCAGGGGCGTATATATGAGTTGTTGCGGTTGAAAAGCTCGTAGTCG	30524	0.1473889950677029	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	29520	0.14254105406888315	No Hit
AAGAACGAAAGTGCCTGACTGACTGTATGCGCTGTAATCTCGTATGCCGT	28296	0.13663081524163678	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG	27676	0.13363706681607082	No Hit
AGCTCCGCCAGTGTATATCTATGCTGCTGCGGTTAAAAAGCTCGTAGTTG	25787	0.12451579137108028	No Hit
AGCTCCAATAGCGTATATTGAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	25564	0.1234390076631751	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	25536	0.12330380612137536	No Hit
AGCTCCACGAGTGTATATTTATGTTGTTGCGGTTAAAAAGTTCGTAGTCG	25181	0.12158964371641419	No Hit
AGCTCTAATAGTATATACGAATCTTGCTGCGGTTAAAAAGCTCGTAGTTG	24212	0.11691070464484414	No Hit
AGCTCTAATAGTATATACGAACATTGCTGCGGTTAAAAAGCTCGTAGTTG	23716	0.11451570590439136	No Hit
AGCTCCAATAGCGTAAATCATAGTTGCTGCGATTAAAAAGCTCGTAGTTG	22316	0.10775562881440368	No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG	22065	0.10654364356469874	No Hit
AGCTCTAATAGTATATACGATCGTTGCTGCGGTTAAAAAGCTCGTAGTTG	21998	0.1062201255896779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0010381546959623942	0.0	0.0
2	0.001047811948948091	0.0	0.0
3	0.0010574692019337877	0.0	0.0
4	0.001062297828426636	0.0	0.0
5	0.0010719550814123327	0.0	0.0
6	0.0010719550814123327	0.0	0.0
7	0.0010816123343980295	0.0	0.0
8	0.0011057554668622712	0.0	0.0
9	0.0011154127198479678	0.0	0.0
10-14	0.0013442896156089794	0.0	0.0
15-19	0.0021110755026732967	0.0	0.0
20-24	0.0022356540661887837	0.0	0.0
25-29	0.00236409553089855	0.0	0.0
30-34	0.0026895449565165286	0.0	0.0
35-39	0.002968639567803163	0.0	0.0
40-44	0.003263185783866912	0.0	0.0
45-49	0.003711282322403238	0.0	0.0
50-54	0.004369906976027753	0.0	0.0
55-59	0.005176287600333426	0.0	0.0
60-64	0.0063023232984656605	0.0	0.0
65-69	0.0076070181768332825	0.0	0.0
70-74	0.009283517295150229	0.0	6.7600770899876846E-6
75-79	0.011364655313567866	0.0	1.4485879478545035E-5
80-84	0.01377413993349919	0.0	1.9314505971393382E-5
85-89	0.016883775394893524	0.0	1.9314505971393382E-5
90-94	0.020384529602208573	0.0	7.436084798986451E-5
95-99	0.024908952626007473	0.0	1.1202413463408162E-4
100-104	0.030735173352278287	0.0	1.1974993702263896E-4
105-109	0.037731853140415536	0.0	1.2071566232120863E-4
110-114	0.046078616895953196	0.0	1.2071566232120863E-4
115-119	0.05561032559283582	0.0	1.2071566232120863E-4
120-124	0.06700105548946507	0.0	1.2361283821691764E-4
125-129	0.0799688147986586	0.0	1.3713299239689303E-4
130-134	0.09383180145962619	0.0	1.42927344188311E-4
135-139	0.10855621508691793	0.0	1.4485879478545036E-4
140-144	0.12386102961865005	0.0	1.4485879478545036E-4
145-149	0.13992104133386365	0.0	1.6417330075684373E-4
150-154	0.15654986524993478	6.277214440702849E-5	1.690019272496921E-4
155-159	0.17371273525611491	6.277214440702849E-5	1.690019272496921E-4
160-164	0.19157961900495238	6.277214440702849E-5	1.690019272496921E-4
165-169	0.20954404100894536	6.277214440702849E-5	1.6996765254826177E-4
170-174	0.2285089544222565	6.277214440702849E-5	1.7383055374254044E-4
175-179	0.24690602136000878	6.470359500416782E-5	1.7383055374254044E-4
180-184	0.2654054551794093	6.760077089987684E-5	1.7383055374254044E-4
185-189	0.2843327053060763	6.760077089987684E-5	1.7383055374254044E-4
190-194	0.30379689869874793	7.339512269129485E-5	1.8348780672823714E-4
195-199	0.3235778499893505	7.725802388557353E-5	1.8348780672823714E-4
200-204	0.3441951193885143	7.725802388557353E-5	1.8928215851965517E-4
205-209	0.3648519835249196	9.07781780655489E-5	2.0280231269963054E-4
210-214	0.385629563323646	9.753825515553658E-5	2.0763093919247882E-4
215-219	0.4059967098704803	1.0526405754409393E-4	2.095623897896182E-4
220-224	0.426632328050317	1.062297828426636E-4	2.124595656853272E-4
225-229	0.44886139297279365	1.062297828426636E-4	2.3273979695529026E-4
230-234	0.4711503328637816	1.062297828426636E-4	2.48191401732405E-4
235-239	0.49317659547355863	1.062297828426636E-4	2.53985753523823E-4
240-244	0.5157542872288189	1.1009268403694226E-4	2.559172041209623E-4
245-249	0.5384159970850547	1.1105840933551194E-4	2.56882929419532E-4
250-254	0.5617865493104407	1.1105840933551194E-4	2.6074583061381064E-4
255-259	0.5851252326009739	1.1105840933551194E-4	2.6074583061381064E-4
260-264	0.6087507363051824	1.1105840933551194E-4	2.6074583061381064E-4
265-269	0.6338219307813494	1.1105840933551194E-4	2.6074583061381064E-4
270-274	0.6588197301348253	1.1105840933551194E-4	2.65574457106659E-4
275-279	0.6843911703156517	1.1105840933551194E-4	2.68471633002368E-4
280-284	0.7111581784161072	1.1105840933551194E-4	2.7040308359950737E-4
285-289	0.7389932786967808	1.1105840933551194E-4	2.7040308359950737E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCAAT	1010955	0.0	294.11002	3
AGCTCTC	495740	0.0	293.74142	1
CCAATAG	957725	0.0	293.6447	5
ATAGCGT	823180	0.0	293.4447	8
TCCAATA	1012155	0.0	293.43634	4
AATAGCG	821675	0.0	293.4329	7
GCTCCAA	1123545	0.0	293.28024	2
AGCTCCA	1207415	0.0	292.99292	1
TAGCGTA	885160	0.0	291.58896	9
CTCTCAT	258305	0.0	289.06702	3
CTCTAAG	167945	0.0	288.50964	5
TCTCATG	235660	0.0	288.38422	4
CTCTCTA	168635	0.0	288.02014	3
GCTCTCA	335690	0.0	287.2579	2
CAATAGC	852750	0.0	287.078	6
CTCATGG	236690	0.0	286.98593	5
AGCTCCG	30650	0.0	286.9633	1
ATGGCGT	239300	0.0	284.55234	8
TCTCTAA	170890	0.0	283.57217	4
AGCTCTA	34405	0.0	282.13852	1
>>END_MODULE