FastQCFastQC Report
Wed 5 Aug 2015
000000000-AFJR0 l01n01.3310000000edf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFJR0 l01n01.3310000000edf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20709823
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG523894225.296894135695897No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG212699210.270449921276487No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG15143557.312254672577357No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG7572123.6562939239026813No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG6704823.237507148177944No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG5674522.7400137606197794No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG4543482.19387678977266No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG4147442.0026438661498944No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG3463221.6722595842562245No Hit
AGCTCTCAAAGTGTATATCCATATTGCTGCGGTTAAAAAGCTCGTAGTTG2881271.3912576655049151No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG2586001.248682811050582No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2466591.19102418209948No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG2200871.062717918931514No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1810590.8742662841686286No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1575510.7607549325747497No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG1556730.7516867720211805No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1546480.746737429866011No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1458850.7044241759091809No Hit
AGCTCCACTAGTATAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1357130.6553073872239276No Hit
AGCTCTCATACTGCATTGAATTGTTGTTGCGGTTAAAAAGCTCGTAGTTG1330060.642236295307787No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1284890.620425389439591No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1221430.5897829257159755No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1148270.5544566942942969No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG898600.43390037664735226No Hit
AGCTCCACTAGTGTAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG841670.4064110060235667No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG822350.39708209963938373No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG808820.3905489679945599No Hit
AGCTCCATTAATATAGATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG733610.35423286814184746No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT635880.3070427014272406No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG607280.2932328296576943No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG599050.28925887005408013No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG560980.27087628899580646No Hit
AGCTCCAATAGCGTATACTAATTTTGTTGCAGTTAAAAAGCTCGTAGTTG556870.26889172350724583No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG519970.25107409174863543No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG507760.2451783388008676No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG471460.22765042463182808No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG466390.225202310999954No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG464240.22416415630399159No Hit
AGCTCTAATAGTGTATATAAATACTGTTGCGGTTAAAAAGCTCGTAGTTG445570.21514911064184372No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG418040.20185590190703223No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG410580.19825374654336736No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG408630.19731216437726193No Hit
AGCTCCAATAGCGTATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG399630.19296640053369843No Hit
AGCTCCAGTAGCGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG397770.19206827600602863No Hit
AGCTCTTCTAGCATATATTAAAGTTGCTGCAGTTAAAACGCTCGTAGTTT388340.18751488122327264No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG380890.18391755448610064No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG373860.18052303006162823No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG311000.15017028392758355No Hit
AGCTCCAGGGGCGTATATATGAGTTGTTGCGGTTGAAAAGCTCGTAGTCG305240.1473889950677029No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG295200.14254105406888315No Hit
AAGAACGAAAGTGCCTGACTGACTGTATGCGCTGTAATCTCGTATGCCGT282960.13663081524163678No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG276760.13363706681607082No Hit
AGCTCCGCCAGTGTATATCTATGCTGCTGCGGTTAAAAAGCTCGTAGTTG257870.12451579137108028No Hit
AGCTCCAATAGCGTATATTGAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG255640.1234390076631751No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG255360.12330380612137536No Hit
AGCTCCACGAGTGTATATTTATGTTGTTGCGGTTAAAAAGTTCGTAGTCG251810.12158964371641419No Hit
AGCTCTAATAGTATATACGAATCTTGCTGCGGTTAAAAAGCTCGTAGTTG242120.11691070464484414No Hit
AGCTCTAATAGTATATACGAACATTGCTGCGGTTAAAAAGCTCGTAGTTG237160.11451570590439136No Hit
AGCTCCAATAGCGTAAATCATAGTTGCTGCGATTAAAAAGCTCGTAGTTG223160.10775562881440368No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG220650.10654364356469874No Hit
AGCTCTAATAGTATATACGATCGTTGCTGCGGTTAAAAAGCTCGTAGTTG219980.1062201255896779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT10109550.0294.110023
AGCTCTC4957400.0293.741421
CCAATAG9577250.0293.64475
ATAGCGT8231800.0293.44478
TCCAATA10121550.0293.436344
AATAGCG8216750.0293.43297
GCTCCAA11235450.0293.280242
AGCTCCA12074150.0292.992921
TAGCGTA8851600.0291.588969
CTCTCAT2583050.0289.067023
CTCTAAG1679450.0288.509645
TCTCATG2356600.0288.384224
CTCTCTA1686350.0288.020143
GCTCTCA3356900.0287.25792
CAATAGC8527500.0287.0786
CTCATGG2366900.0286.985935
AGCTCCG306500.0286.96331
ATGGCGT2393000.0284.552348
TCTCTAA1708900.0283.572174
AGCTCTA344050.0282.138521
Produced by FastQC (version 0.11.2)