FastQCFastQC Report
Tue 16 Jun 2015
000000000-AFC60 l01n03.3330000000c8fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFC60 l01n03.3330000000c8fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23229140
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAACCCTCTAGGTAGATGCTTTCGCCTTCGTTTCTCTCTCATGGGTC9133953.9321085498645236No Hit
CAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTCTCTCTCATGAGTC6845892.9471129796453934No Hit
CAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGATTCTCTCTCATGAGTC4341281.868893984021793No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC2001310.8615514823191904No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC1804370.7767700397001353No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATC1653060.7116320277031349No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC1234910.5316210587219329No Hit
TGATGGTGGAATTACCGCGGCTGCTGGGTAACGGCGATAGTGTAGATCTC1035130.44561701380249114No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC849180.36556669769091754No Hit
TGATGGTGGAATTACCGCAGCTGCTGGGTAACGGCGATAGTGTAGATCTC815930.35125277991350523No Hit
TGATGGTGGAATTACCGCGGGTGCTGGGTAACGGCGATAGTGTAGATCTC728290.3135243061086204No Hit
TGATGGTGGAATTACCGCAGGTGCTGGGTAACGGCGATAGTGTAGATCTC698760.30081182514720733No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC645810.27801718014528304No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC553860.23843327820143148No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTCCGTCTTTAACAAATC401380.17279158849617335No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC400880.17257634161230248No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC399450.1719607355244318No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC377460.16249417757179127No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC367600.1582495090218579No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCAAAAATC367000.15799121276121286No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCAAATC364800.15704412647218105No Hit
TAGAAATACACTTGATAGATGCTTTCGCCTTCGTTTCTCTCTCATGGGTC360940.155382420528698No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC358960.1545300428685694No Hit
CGAAACCATCCTTGGCAAATGCTTTCGCCCCCGCTGGTCTTTGGCAAATC345620.1487872560068948No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC338580.14575657988199303No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGATGTTAGTCTTCCGTAAATC295020.12700427135916353No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC293890.1265178134016154No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA291530.1255018481097449No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCTATAAATC288440.12417162236742299No Hit
AACTAGAAGGACATATAGGGACACATTTGTATTCAGAAAATGGACTGAAA287070.12358184590561681No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC270160.11630219629310426No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATA262590.11304335847129941No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCAGAAATC259710.11180353642020326No Hit
TGAAAACATCCTTGGCAAATGCCTTCGCATTAGTTCGTCTTTAAGAAATC241660.10403312391246511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAC7390800.0317.86451
GAAACCC2934900.0304.018043
GGTGGAA702700.0303.38485
TGGTGGA667150.0300.69154
TCCTTGG5532400.0290.970559
CTCTAGG3089500.0288.097759
GAAGACA1736800.0286.971132
ATCCTTG5629900.0284.60898
ATTCTTG3775950.0283.60188
GTGGAAT757250.0280.8566
TTCTTGG3795900.0275.240029
AAACATC5578300.0274.33324
CAGAAAC3303950.0273.498721
AGAAACC3298150.0271.647772
CATCCTT5934300.0270.907787
AAGACAT1776800.0270.491153
TGAAGAC809800.0266.412871
AACATCC5133600.0265.753975
ACATCCT5846700.0265.42926
CGAAGAC748350.0264.543761
Produced by FastQC (version 0.11.2)