##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFC60 l01n03.3330000000c8fe.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23229140
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.262992344959823	31.0	21.0	33.0	12.0	34.0
2	29.20936500447283	33.0	27.0	34.0	11.0	34.0
3	29.172367982628714	34.0	27.0	34.0	11.0	34.0
4	28.66869130755594	33.0	23.0	34.0	11.0	34.0
5	27.60588123365738	32.0	23.0	34.0	11.0	34.0
6	29.53470864612293	34.0	26.0	37.0	11.0	38.0
7	29.760004632112942	36.0	26.0	37.0	11.0	38.0
8	31.005686176931217	34.0	27.0	37.0	11.0	38.0
9	31.209597126712396	34.0	27.0	37.0	11.0	38.0
10-14	31.122779836016313	35.6	27.8	37.0	13.0	38.0
15-19	31.41192991217066	35.8	28.8	37.0	11.2	38.0
20-24	30.67137186309954	35.6	26.4	37.6	11.0	38.0
25-29	31.93650092082617	36.2	30.8	37.8	13.0	38.0
30-34	29.53624454456773	34.2	23.8	37.0	11.0	38.0
35-39	30.38398481390185	34.4	26.4	37.2	11.0	38.0
40-44	28.782691593403804	33.2	21.2	37.0	11.0	38.0
45-49	28.379884576010994	31.8	20.8	36.6	11.0	37.8
50-54	27.015090296067786	31.4	17.2	36.8	11.0	37.4
55-59	29.318612897421083	34.2	24.0	37.0	11.0	38.0
60-64	28.91565981349288	33.6	23.4	36.6	11.0	38.0
65-69	27.9880582234211	31.4	20.4	36.4	11.0	37.8
70-74	25.240811067478177	28.2	11.0	36.6	9.2	37.6
75-79	27.612292861466244	31.0	19.8	36.4	11.0	38.0
80-84	26.18508197892819	28.8	15.4	35.8	11.0	37.6
85-89	24.950857552195213	26.4	14.0	34.8	10.2	37.0
90-94	25.58979732353415	28.4	14.8	35.6	10.8	37.6
95-99	25.83304238555538	28.0	14.2	35.8	11.0	37.6
100-104	25.35859213040173	27.8	13.8	35.6	10.6	37.2
105-109	24.52858341720787	26.4	14.6	34.4	9.8	36.6
110-114	25.517596363877438	27.0	14.6	35.6	11.0	37.4
115-119	22.595631951936234	22.6	11.0	33.2	11.0	37.0
120-124	24.346426419574723	25.6	11.0	34.8	11.0	37.2
125-129	24.45120395331037	25.6	11.0	34.4	10.6	37.0
130-134	23.103408511894976	23.6	11.0	32.8	10.6	36.8
135-139	21.882588128531662	22.0	11.0	30.8	10.2	36.8
140-144	20.65790140315139	19.8	10.4	29.6	9.6	36.0
145-149	19.654193810016213	18.0	9.4	27.0	8.4	35.4
150-154	20.037128623788917	18.6	9.6	28.2	9.0	35.4
155-159	19.17860215229664	17.2	9.4	26.8	9.2	35.0
160-164	18.836261833197444	17.0	9.2	26.0	8.6	35.0
165-169	17.604263980500352	15.6	9.2	25.0	8.2	34.6
170-174	17.262206246120176	15.2	9.4	24.2	8.4	32.6
175-179	18.17799283141778	16.4	9.8	25.8	8.8	33.6
180-184	19.000701291567403	17.6	10.0	26.6	9.2	34.0
185-189	17.443199636318866	15.0	9.4	24.2	8.6	32.8
190-194	16.371462714504283	14.4	9.2	22.0	8.2	29.4
195-199	15.598675827000054	14.0	9.0	20.6	8.0	27.8
200-204	16.00403803154142	14.8	9.0	22.0	8.0	28.6
205-209	15.575941149779975	13.0	9.0	21.0	8.0	28.0
210-214	14.986049780577327	11.8	9.0	19.8	8.0	26.2
215-219	14.718981580893654	10.8	9.0	19.4	8.0	26.4
220-224	14.119910784471575	9.0	8.6	18.2	8.0	25.4
225-229	13.820519597367788	9.0	8.0	17.8	7.8	25.0
230-234	13.31465693521155	9.0	8.0	16.4	7.0	24.4
235-239	14.116257950143654	10.2	8.6	17.8	7.6	24.8
240-244	12.708039677749584	9.0	8.0	15.8	7.0	22.8
245-249	12.252900081535518	9.0	8.0	15.2	7.0	21.4
250-254	12.07020931467975	9.0	8.0	14.8	7.0	21.2
255-259	10.082789634054468	8.4	6.2	12.8	6.0	16.6
260-264	10.825177927379146	8.6	7.2	13.6	7.0	18.2
265-269	10.931925684721863	9.0	7.2	13.4	7.0	18.2
270-274	10.760194032150995	9.0	7.0	13.2	7.0	17.8
275-279	10.863008230179851	9.0	7.8	13.2	7.0	17.6
280-284	11.152550925260254	9.0	8.0	13.8	7.0	17.6
285-289	10.911215972696363	9.0	8.0	13.6	7.0	17.0
290-294	11.608611726478037	9.2	8.2	14.8	7.2	18.0
295-299	11.700475084312206	9.4	8.4	15.0	7.2	17.8
300-301	12.036378445349246	11.0	9.0	15.5	7.5	17.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5269079577398017
1101	2	1.0365331157197417
1101	3	2.080441436600534
1101	4	1.211115386580449
1101	5	2.3790219477325287
1101	6	4.464807598130999
1101	7	3.9615805218436932
1101	8	2.9647277496869897
1101	9	2.7779084962152645
1101	10-14	2.050444926602186
1101	15-19	2.4580930113583115
1101	20-24	3.7558339362781297
1101	25-29	2.044721893315341
1101	30-34	3.084823877837202
1101	35-39	2.4844293447652532
1101	40-44	2.543177302835538
1101	45-49	2.578076012012925
1101	50-54	4.554844292862821
1101	55-59	2.3028011935249033
1101	60-64	1.1235075445176363
1101	65-69	1.7414958410322434
1101	70-74	3.4867696021971746
1101	75-79	3.192167876013105
1101	80-84	2.8503583329994484
1101	85-89	1.7054696846114155
1101	90-94	2.3827369995611143
1101	95-99	3.3510633732951547
1101	100-104	2.115278353114604
1101	105-109	2.3608516940997752
1101	110-114	1.788471152216971
1101	115-119	1.6373553768685554
1101	120-124	1.6859379354921131
1101	125-129	1.7822314285101157
1101	130-134	1.5090232420281815
1101	135-139	1.3391155441130067
1101	140-144	0.9789478448489461
1101	145-149	1.6844143484429566
1101	150-154	1.6156881792113431
1101	155-159	1.7813806407467858
1101	160-164	1.312045669888306
1101	165-169	1.0860181464021927
1101	170-174	0.8243079728795557
1101	175-179	-0.008483527604685293
1101	180-184	-0.4298917744787225
1101	185-189	-6.108580149829731
1101	190-194	-0.11313567184054207
1101	195-199	0.8548327839656213
1101	200-204	1.1669555769852487
1101	205-209	1.5652774501096225
1101	210-214	0.7179164427314024
1101	215-219	0.35778677347781596
1101	220-224	0.37931802077248733
1101	225-229	0.13893341670221382
1101	230-234	0.15300292469279242
1101	235-239	-0.8099720509548174
1101	240-244	0.04110768875137083
1101	245-249	-0.02094047743302596
1101	250-254	0.061450768886901486
1101	255-259	1.686719487116921
1101	260-264	0.597649265588025
1101	265-269	0.08107999861910109
1101	270-274	0.03083903337378935
1101	275-279	-0.22503065756558804
1101	280-284	-0.6805727002968407
1101	285-289	-0.7832182903581391
1101	290-294	-1.438681321206781
1101	295-299	-0.14672853204641
1101	300-301	0.703134895185153
1102	1	1.6040423483280186
1102	2	1.5970113495974445
1102	3	1.9400953113705413
1102	4	1.88072103158186
1102	5	1.9780984233548153
1102	6	3.9157408793863517
1102	7	4.168460925612408
1102	8	2.486657618166287
1102	9	2.654060956664768
1102	10-14	2.100741502563036
1102	15-19	2.3273863095692953
1102	20-24	3.022262971538069
1102	25-29	1.700346119721189
1102	30-34	2.234172322090277
1102	35-39	1.5268913487722031
1102	40-44	1.7060004676210276
1102	45-49	1.8795221673612446
1102	50-54	3.4885524406083235
1102	55-59	2.5487733118273113
1102	60-64	2.1697382821106572
1102	65-69	1.8398451134734017
1102	70-74	2.795529198233197
1102	75-79	2.760223468988926
1102	80-84	2.351960210529402
1102	85-89	0.8605939097097277
1102	90-94	1.840914630921219
1102	95-99	2.466643590468756
1102	100-104	2.528096184112151
1102	105-109	2.153232681160585
1102	110-114	1.601485174537622
1102	115-119	1.3115329360344923
1102	120-124	1.3140700636525224
1102	125-129	1.7264779023138637
1102	130-134	1.5770474953702873
1102	135-139	1.364481169917358
1102	140-144	0.9872203516064744
1102	145-149	1.522348976796092
1102	150-154	2.1340191548364267
1102	155-159	1.620143370605394
1102	160-164	1.2281804144679995
1102	165-169	0.8999982880844541
1102	170-174	0.8381960620649203
1102	175-179	0.5454136014088604
1102	180-184	0.5627401070399713
1102	185-189	-1.0073661892690247
1102	190-194	0.9518623356925247
1102	195-199	1.0504604652877756
1102	200-204	1.480444507929608
1102	205-209	1.9316877128434413
1102	210-214	0.8999558933902048
1102	215-219	0.5213802224860036
1102	220-224	0.7431891751943596
1102	225-229	0.554227717571715
1102	230-234	0.4632848111528389
1102	235-239	0.11566121383616768
1102	240-244	0.4937997984130096
1102	245-249	0.4009886881808242
1102	250-254	0.3575377191085458
1102	255-259	1.9403714702461663
1102	260-264	0.759336556158452
1102	265-269	0.7403030397439085
1102	270-274	0.38492141406896785
1102	275-279	0.17392940354632636
1102	280-284	-0.27949167492828053
1102	285-289	-0.4096213885102191
1102	290-294	0.6116483024529042
1102	295-299	0.5526515304172488
1102	300-301	0.15260428579415297
1103	1	1.055363141035759
1103	2	1.0867310020040364
1103	3	1.3733970522416215
1103	4	1.7989989791894452
1103	5	1.4849873781926846
1103	6	3.432956541377191
1103	7	4.319010540938848
1103	8	2.05609952172151
1103	9	2.014064709948986
1103	10-14	1.8859060443308913
1103	15-19	1.7816656165626377
1103	20-24	2.1945834844211376
1103	25-29	1.305994900569715
1103	30-34	1.7970269679347766
1103	35-39	0.8905514418107856
1103	40-44	1.2053736936225654
1103	45-49	1.49148216448107
1103	50-54	2.6057144265873937
1103	55-59	1.9447910942624667
1103	60-64	2.0223346792522285
1103	65-69	1.264632953422229
1103	70-74	2.1345721366511476
1103	75-79	2.1798917186557034
1103	80-84	1.7817608502053908
1103	85-89	-0.016021436759100283
1103	90-94	1.1963791263214496
1103	95-99	1.7531472890571038
1103	100-104	2.113137908483708
1103	105-109	1.7897949337560313
1103	110-114	1.1907910330537206
1103	115-119	0.8069080856052864
1103	120-124	0.8342093781458786
1103	125-129	1.1053751734658803
1103	130-134	1.1999262506302202
1103	135-139	0.9567313112036224
1103	140-144	0.7747339138834235
1103	145-149	1.391939669592663
1103	150-154	1.716411143573989
1103	155-159	1.4803635067181276
1103	160-164	0.6915673911445843
1103	165-169	0.664938264929944
1103	170-174	0.8097193646642111
1103	175-179	0.09627978374964741
1103	180-184	0.690911105754406
1103	185-189	0.640884915227435
1103	190-194	0.3187726506315478
1103	195-199	1.0390906792664136
1103	200-204	1.3812496559477658
1103	205-209	2.0505357462358518
1103	210-214	1.5128571692532002
1103	215-219	0.6234733383344757
1103	220-224	0.4501770335266073
1103	225-229	0.4462811205632722
1103	230-234	0.4634910514739854
1103	235-239	0.2097181994129329
1103	240-244	0.09945351847653328
1103	245-249	0.40300342761607766
1103	250-254	0.42522655870722126
1103	255-259	-1.4926430662242876
1103	260-264	0.18527448688397108
1103	265-269	0.33071295618027285
1103	270-274	0.3882580553585768
1103	275-279	0.03188349412214997
1103	280-284	-0.5849492125953866
1103	285-289	-0.6410535324535029
1103	290-294	-1.307507895339457
1103	295-299	0.09173590929788311
1103	300-301	-0.2390381448192791
2116	1	0.040629610370846336
2116	2	0.8925240446259295
2116	3	1.2182356423675067
2116	4	1.2519200887151172
2116	5	0.5649603915603088
2116	6	2.706866276568359
2116	7	3.9720186282820933
2116	8	1.7378184366605574
2116	9	1.8391349764400253
2116	10-14	1.7768872082381186
2116	15-19	1.2968294526930642
2116	20-24	1.5357842762775107
2116	25-29	1.0434113064827457
2116	30-34	1.395846456759127
2116	35-39	0.531623624043938
2116	40-44	0.905587866148295
2116	45-49	1.2707277139823319
2116	50-54	1.7483514003946077
2116	55-59	1.7767841512746152
2116	60-64	1.8831503690360982
2116	65-69	1.3652044479429115
2116	70-74	1.831857916502667
2116	75-79	1.6273173838718655
2116	80-84	1.3779546792095942
2116	85-89	-0.1642545638306565
2116	90-94	0.9965442611774051
2116	95-99	1.257960496699205
2116	100-104	1.985388570537566
2116	105-109	1.49793850979853
2116	110-114	0.9596652344283605
2116	115-119	0.6231845898363915
2116	120-124	0.8053501256113904
2116	125-129	0.8925172931258345
2116	130-134	1.0795662279816938
2116	135-139	0.7679091323061193
2116	140-144	0.9927155301949071
2116	145-149	1.2668835158280025
2116	150-154	1.553759238748576
2116	155-159	1.2117516682377065
2116	160-164	0.8392796525484556
2116	165-169	0.6631857980745011
2116	170-174	0.6816566785319296
2116	175-179	0.3484219872941594
2116	180-184	0.7243165879766522
2116	185-189	0.4025674724860622
2116	190-194	1.0206036871185908
2116	195-199	1.0281481748458265
2116	200-204	1.3603770398764112
2116	205-209	1.5693166328119261
2116	210-214	0.6842582348590032
2116	215-219	0.31017752703424506
2116	220-224	0.3924312769243574
2116	225-229	0.4676300279242618
2116	230-234	0.49616090707507254
2116	235-239	0.29822802854352837
2116	240-244	0.414263202403367
2116	245-249	0.4142960688821109
2116	250-254	0.3767566659991939
2116	255-259	-1.6059546022480742
2116	260-264	0.35921595619639923
2116	265-269	0.580353992918516
2116	270-274	0.4074863547338108
2116	275-279	0.1517956484495162
2116	280-284	-0.3234001633933872
2116	285-289	-0.46271419551653103
2116	290-294	-1.2502248226892299
2116	295-299	-1.5228073120367185
2116	300-301	-1.7847121434330777
1117	1	0.3797613132156492
1117	2	0.5203556683503479
1117	3	0.9221547859976553
1117	4	0.958848790800463
1117	5	0.4430010623321685
1117	6	2.435447019926894
1117	7	3.832355308040988
1117	8	1.1926303653516932
1117	9	1.3873447784592834
1117	10-14	1.3376394731041863
1117	15-19	1.2249185606477972
1117	20-24	1.5195080119173667
1117	25-29	0.8982569138245395
1117	30-34	1.29727644957525
1117	35-39	0.4349486972848098
1117	40-44	0.7953592401774685
1117	45-49	1.1733796981316011
1117	50-54	1.3410210319148135
1117	55-59	1.4121454989229747
1117	60-64	1.5512991387606156
1117	65-69	1.166050966668049
1117	70-74	1.6113439285474769
1117	75-79	1.5178335484168137
1117	80-84	1.237839999925793
1117	85-89	-0.032155175962888194
1117	90-94	1.0901868344271186
1117	95-99	0.8579734584171028
1117	100-104	1.9996616949637556
1117	105-109	1.2125176516558334
1117	110-114	0.8721137575819853
1117	115-119	0.4762825908912376
1117	120-124	0.6246574290231166
1117	125-129	0.8199778453065676
1117	130-134	0.6394525323553601
1117	135-139	0.6504839006987204
1117	140-144	0.7255491610717186
1117	145-149	0.9374604888957379
1117	150-154	1.3094827034678431
1117	155-159	0.8984029994586322
1117	160-164	1.0598789591475004
1117	165-169	0.4659187149697068
1117	170-174	0.7017930646992099
1117	175-179	0.35065124430837713
1117	180-184	-0.0819497120439685
1117	185-189	-0.15756868093895804
1117	190-194	0.8984190417691451
1117	195-199	0.8093923469451791
1117	200-204	1.1733515488861173
1117	205-209	2.0953676524678038
1117	210-214	0.522756395312669
1117	215-219	0.6549936593997447
1117	220-224	0.5996317783302736
1117	225-229	0.4701858788107032
1117	230-234	0.7482275598693171
1117	235-239	0.7518909823772191
1117	240-244	0.24356545244418726
1117	245-249	0.2824044118228386
1117	250-254	0.439754884905053
1117	255-259	-1.460061142314407
1117	260-264	0.3339687584864848
1117	265-269	0.7732180111847651
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2106	125-129	-0.63361790293159
2106	130-134	-0.21502613795868797
2106	135-139	-0.4641024180482489
2106	140-144	0.1745122693993295
2106	145-149	-0.6715608069858163
2106	150-154	-1.1611467702075302
2106	155-159	-1.862078373464449
2106	160-164	-0.6671658170467794
2106	165-169	-0.1279632429262918
2106	170-174	0.10726360904601151
2106	175-179	0.4583924540901023
2106	180-184	0.1840139392729938
2106	185-189	0.15207602663071995
2106	190-194	-0.270259554114304
2106	195-199	-0.16187731455872267
2106	200-204	-0.44720905799468547
2106	205-209	-0.41210817539803557
2106	210-214	-1.575751564030341
2106	215-219	-1.088962089592668
2106	220-224	-0.46990151466905594
2106	225-229	-0.43757618296511325
2106	230-234	-0.3709777031904711
2106	235-239	-1.2562850362365996
2106	240-244	-0.38323590588193923
2106	245-249	-0.342590896358816
2106	250-254	-0.29831267944505946
2106	255-259	1.3946033807207545
2106	260-264	0.11007140753436317
2106	265-269	0.04057592922211661
2106	270-274	-0.19108494670429543
2106	275-279	-0.33628927414730825
2106	280-284	1.276672754483764
2106	285-289	0.6517983543691628
2106	290-294	0.929834318923449
2106	295-299	1.5539804118277019
2106	300-301	-0.012556603831557211
2105	1	-1.1920754787405201
2105	2	-0.47285889016697524
2105	3	-0.8401324043229899
2105	4	-0.5780264251451435
2105	5	-1.2148566908913452
2105	6	0.8889035555272073
2105	7	2.1543642428280982
2105	8	0.3441940963493124
2105	9	-1.3259365680685953
2105	10-14	-0.8011262317198167
2105	15-19	-0.9627361000529753
2105	20-24	-1.1781301583443735
2105	25-29	-0.7560864093195114
2105	30-34	-1.1914016563121912
2105	35-39	-0.6844107550312017
2105	40-44	-1.1676490993311077
2105	45-49	-0.6625822647498332
2105	50-54	-8.021751824662832
2105	55-59	-1.2906148160344415
2105	60-64	-0.45052182793013174
2105	65-69	-0.9577826690282016
2105	70-74	-0.5508403668611486
2105	75-79	-0.8374609159845008
2105	80-84	-1.2019624863716523
2105	85-89	-2.4913878862373267
2105	90-94	-1.0945920632505626
2105	95-99	-1.1005395324602496
2105	100-104	0.15772976181809284
2105	105-109	-1.5431346434148665
2105	110-114	-0.9985676826310588
2105	115-119	-0.4789436382901826
2105	120-124	-0.7207271515729161
2105	125-129	-0.37108652705249057
2105	130-134	-0.2722253789231246
2105	135-139	-0.560830058636828
2105	140-144	0.2060379721692378
2105	145-149	-2.545893044328274
2105	150-154	-0.8846107025981915
2105	155-159	-0.6364960092835013
2105	160-164	-0.15476803952100937
2105	165-169	-0.1577791568710083
2105	170-174	-0.019633004950815547
2105	175-179	0.32675938617783373
2105	180-184	-0.34854981470907376
2105	185-189	0.22135865234971064
2105	190-194	-0.30063648743012905
2105	195-199	-0.06196078559311502
2105	200-204	-0.4185410297225829
2105	205-209	-0.23941521980677294
2105	210-214	-0.8915088041223154
2105	215-219	-0.6850804324487054
2105	220-224	-0.42061703793952177
2105	225-229	-0.28904768146280624
2105	230-234	-0.3447939472979673
2105	235-239	-0.016359410383966377
2105	240-244	0.19583021211211005
2105	245-249	0.006432033916510704
2105	250-254	-0.2901026224325207
2105	255-259	1.4810635646736188
2105	260-264	0.23259769870357339
2105	265-269	0.06923688611083101
2105	270-274	-0.2848577818648046
2105	275-279	-0.39796260135294936
2105	280-284	-0.8716950261764378
2105	285-289	-0.1934151006077407
2105	290-294	1.3060920435425114
2105	295-299	1.4186694537688336
2105	300-301	1.1822667254794297
2104	1	-1.0974893417189158
2104	2	0.025726047176910072
2104	3	-0.16881668402389494
2104	4	0.1974898329368031
2104	5	-0.6306830718527969
2104	6	0.016567667294758337
2104	7	-27.73525787214613
2104	8	-7.017558559599372
2104	9	-4.849127420162702
2104	10-14	-0.6517242880158669
2104	15-19	-0.6895132898146521
2104	20-24	-0.49285023322062216
2104	25-29	-0.4245600720071714
2104	30-34	-0.6316159744038572
2104	35-39	-0.3237866157286575
2104	40-44	-0.673638497064907
2104	45-49	-1.519491077510235
2104	50-54	-0.565522752096058
2104	55-59	-0.3953692753210234
2104	60-64	-0.84443987460293
2104	65-69	-0.607543668468999
2104	70-74	-0.4008133368143092
2104	75-79	-0.6677566552179428
2104	80-84	-1.3288536202204888
2104	85-89	-0.5232940538940412
2104	90-94	-1.1119889072746965
2104	95-99	-0.9164869109034051
2104	100-104	-0.3240648529260426
2104	105-109	-0.8625795225563522
2104	110-114	-0.9157219208195571
2104	115-119	-0.6966981452624665
2104	120-124	-0.7984929081745058
2104	125-129	-0.460980205251456
2104	130-134	-0.3386519569783353
2104	135-139	-0.4895480066012752
2104	140-144	-0.1773745094305923
2104	145-149	-3.6060888577813515
2104	150-154	-1.227042879795274
2104	155-159	-1.0227460085569149
2104	160-164	-0.11607964259507142
2104	165-169	-0.3455578026076296
2104	170-174	-2.462294826297331
2104	175-179	-0.4466445297553818
2104	180-184	-0.9547932194687121
2104	185-189	0.08224541364506521
2104	190-194	-0.815395350594212
2104	195-199	0.003716850939520455
2104	200-204	-0.4797819597769042
2104	205-209	-0.3811482322572637
2104	210-214	0.04306458768252419
2104	215-219	-0.3472298193967074
2104	220-224	-0.4050482505505073
2104	225-229	-0.428239435387324
2104	230-234	-0.3800274828380861
2104	235-239	0.00573169031091858
2104	240-244	-0.48895272490776875
2104	245-249	-0.3814817110316824
2104	250-254	-0.3560453542639159
2104	255-259	1.4060601230140701
2104	260-264	0.3515469408721632
2104	265-269	-0.27881291692997046
2104	270-274	0.011119262363960658
2104	275-279	1.1021499781479012
2104	280-284	0.8306057194106167
2104	285-289	0.9170465643339512
2104	290-294	0.7340034100179516
2104	295-299	0.5821955754728787
2104	300-301	0.799762935380512
2111	1	-1.0764039490103272
2111	2	-0.14490318371779765
2111	3	0.3939071557934106
2111	4	0.6226469979315041
2111	5	0.027631910611926713
2111	6	-27.543891349098683
2111	7	-5.27092380477805
2111	8	-4.004407348442044
2111	9	-2.5287440909073418
2111	10-14	-0.3519688031451409
2111	15-19	-0.2664662137197169
2111	20-24	0.02427329667090916
2111	25-29	-0.47769805422721845
2111	30-34	-0.37488901901685523
2111	35-39	-0.21855521535348288
2111	40-44	-0.4898602571171011
2111	45-49	-1.2015487563292453
2111	50-54	0.3259116007299774
2111	55-59	-0.1600945505840521
2111	60-64	-0.8232609169322593
2111	65-69	-0.4258814223226821
2111	70-74	-0.03437505282442288
2111	75-79	-0.5385685955164483
2111	80-84	-0.5362125468985752
2111	85-89	-1.990497675132115
2111	90-94	-0.745123790236935
2111	95-99	-0.4925529087828586
2111	100-104	0.34353677826227624
2111	105-109	-0.6802311007310564
2111	110-114	-0.8863834818667833
2111	115-119	-0.43249197937061723
2111	120-124	-0.7570703488400277
2111	125-129	-0.1270821708333827
2111	130-134	-0.3524592810138927
2111	135-139	-0.22309947874873615
2111	140-144	0.31754993392639363
2111	145-149	-3.700631978244612
2111	150-154	-1.0989726408261795
2111	155-159	-0.5916552655506351
2111	160-164	-0.23899532647077137
2111	165-169	-0.17220619996510322
2111	170-174	-2.6342600330504524
2111	175-179	-0.8486497821817807
2111	180-184	-0.6056086341983438
2111	185-189	0.16477845336449803
2111	190-194	-0.5272486252047646
2111	195-199	-0.26560514563498394
2111	200-204	-0.24491488531315753
2111	205-209	-0.03128112516975179
2111	210-214	-0.39696710630654763
2111	215-219	-0.36900510689364907
2111	220-224	-0.49988754587715256
2111	225-229	-0.40817056661642503
2111	230-234	-0.35327940356233256
2111	235-239	0.3446781873340008
2111	240-244	-0.5780469423451731
2111	245-249	-0.3871763688899321
2111	250-254	-0.31761437799770853
2111	255-259	1.3551478320100738
2111	260-264	0.16210015839112657
2111	265-269	-0.42593517449460805
2111	270-274	-0.009510033778280302
2111	275-279	2.072546242726647
2111	280-284	1.3808742945676205
2111	285-289	1.2318384247042324
2111	290-294	0.4801049514909135
2111	295-299	-2.0331713114042103
2111	300-301	-1.5719138854334513
2110	1	0.23163551720401898
2110	2	0.5245383890548467
2110	3	0.17078547691409085
2110	4	0.5698424366098784
2110	5	0.3934511759712649
2110	6	-27.543891349098683
2110	7	-5.376499933759437
2110	8	-3.7392784750909662
2110	9	-1.7793132792427393
2110	10-14	0.09440348839547852
2110	15-19	0.11838058934764106
2110	20-24	0.2091331971794439
2110	25-29	-0.5626625923521047
2110	30-34	-0.02308810635430092
2110	35-39	-0.2163382133084859
2110	40-44	-0.29507269245928214
2110	45-49	-1.1649438790458113
2110	50-54	0.4977189385217109
2110	55-59	-0.1447840245751877
2110	60-64	-0.7627282352934586
2110	65-69	-0.3662498349116987
2110	70-74	0.10641997618769494
2110	75-79	-0.3271410294593622
2110	80-84	-0.5974910916805669
2110	85-89	-0.04327737777540008
2110	90-94	-0.662716015294567
2110	95-99	-0.4000067319733951
2110	100-104	0.012687159166773654
2110	105-109	-0.6620255658816312
2110	110-114	-0.434586397768566
2110	115-119	-0.21361956058023068
2110	120-124	-0.28854273216439097
2110	125-129	0.012692841046980874
2110	130-134	-0.438690002568876
2110	135-139	-0.19851662822516047
2110	140-144	0.12645842315511402
2110	145-149	-3.3667952854357495
2110	150-154	-1.8460929557366832
2110	155-159	-0.6940438397236974
2110	160-164	-0.12909328089662608
2110	165-169	-0.6611162210243364
2110	170-174	-2.647696482422301
2110	175-179	-1.1186967890090607
2110	180-184	-0.5570517728537148
2110	185-189	0.13848788147467417
2110	190-194	-0.49723826039615204
2110	195-199	-0.2156871530932012
2110	200-204	-0.48599039022380275
2110	205-209	-0.21299754099637447
2110	210-214	-0.3318784223486997
2110	215-219	-0.42232239576566677
2110	220-224	-0.43285740002542106
2110	225-229	-0.4098502248220619
2110	230-234	-0.22794983428931914
2110	235-239	0.4772995249275205
2110	240-244	-0.6102329091369523
2110	245-249	-0.43100218593381356
2110	250-254	-0.3610653836341733
2110	255-259	1.1605592169895296
2110	260-264	-0.8670157072470452
2110	265-269	-0.7044814992382822
2110	270-274	-0.41509381235195164
2110	275-279	-0.48373505289641194
2110	280-284	-0.9143776272732396
2110	285-289	0.8492865854985219
2110	290-294	0.7056504870563973
2110	295-299	0.42732390235576645
2110	300-301	-1.97608439374328
2109	1	-0.5969940656619244
2109	2	-0.7486716890956728
2109	3	0.2514738262857392
2109	4	-0.7587058413317536
2109	5	-0.26934520889833635
2109	6	-8.534468220745921
2109	7	-1.077935676667341
2109	8	-0.5326978333281112
2109	9	-0.6660090168876778
2109	10-14	0.013387028377550791
2109	15-19	-1.1799279695110698
2109	20-24	-8.941578822108504
2109	25-29	-1.7725133020958772
2109	30-34	-0.5763200554728343
2109	35-39	-0.3006105922377529
2109	40-44	-0.15897689566176254
2109	45-49	-0.5509658571605627
2109	50-54	-7.441989928151678
2109	55-59	-0.29371389330122355
2109	60-64	-1.410997685323867
2109	65-69	-0.8154167571708157
2109	70-74	-0.017269151577458786
2109	75-79	-0.8343779401562372
2109	80-84	-0.6500794966740635
2109	85-89	-0.878906629756294
2109	90-94	-0.9000134275755975
2109	95-99	-0.6345416311198697
2109	100-104	-0.060782605177639226
2109	105-109	-0.7462362250365295
2109	110-114	-0.8289419707629904
2109	115-119	-0.3823065634359324
2109	120-124	-0.5585980524304581
2109	125-129	-0.21963472039192666
2109	130-134	-0.3670024897194395
2109	135-139	-0.30468489926158426
2109	140-144	0.10360215368489634
2109	145-149	-0.22507082373079612
2109	150-154	-0.6878365444119474
2109	155-159	-0.8719497733725099
2109	160-164	-0.4136992592277835
2109	165-169	-0.6260857268037867
2109	170-174	-0.4508174724171994
2109	175-179	-0.0691724598314849
2109	180-184	-0.6728829135490777
2109	185-189	0.1980468693441857
2109	190-194	-0.27686056362489353
2109	195-199	-0.35779709549159655
2109	200-204	-0.16545213341840004
2109	205-209	-0.3488674606846356
2109	210-214	-0.45872016046110176
2109	215-219	-0.06446958618981746
2109	220-224	-0.43121971961103434
2109	225-229	-0.5753507304272532
2109	230-234	-0.6180713025125364
2109	235-239	-1.3992374203756999
2109	240-244	-0.5792474781654029
2109	245-249	-0.6532825158621094
2109	250-254	-0.471059664091138
2109	255-259	1.3528232761812333
2109	260-264	-0.41084045340164543
2109	265-269	1.0272797685468493
2109	270-274	1.3530430003484337
2109	275-279	-0.4182623256379312
2109	280-284	-0.11338892958113966
2109	285-289	1.912570244653697
2109	290-294	1.0387938936357273
2109	295-299	0.5812618097572422
2109	300-301	1.2977754554090755
2108	1	0.3754196430869001
2108	2	0.5636391223126296
2108	3	0.11073291925693596
2108	4	-0.11149404131231933
2108	5	0.3091743555224298
2108	6	1.741085790026073
2108	7	2.182230697416248
2108	8	0.9168805940663987
2108	9	0.35908423834828795
2108	10-14	0.36326882695238183
2108	15-19	-0.24488756356694097
2108	20-24	0.14520862255777445
2108	25-29	-0.06989296061527739
2108	30-34	0.011909260361804286
2108	35-39	0.2954200872727064
2108	40-44	0.22782840017358552
2108	45-49	-0.7060189035320121
2108	50-54	0.3192516330159947
2108	55-59	-0.26183573996964427
2108	60-64	-0.8517707073384102
2108	65-69	-0.27231484770468484
2108	70-74	0.35599721024054887
2108	75-79	-0.4479363963064884
2108	80-84	-0.3198518084827384
2108	85-89	0.05685546158389343
2108	90-94	-0.43569687485187103
2108	95-99	-0.03014412840830616
2108	100-104	0.5904437892343388
2108	105-109	-0.775016706010863
2108	110-114	-0.5296116253949457
2108	115-119	-0.248907878534407
2108	120-124	-0.4141122719247612
2108	125-129	-0.17659640167295265
2108	130-134	-0.255343229156594
2108	135-139	-0.10054695296656035
2108	140-144	0.49449212225768946
2108	145-149	-0.04590837143898341
2108	150-154	-0.10343134121018238
2108	155-159	-0.14774293973872332
2108	160-164	-1.136557581582764
2108	165-169	-5.724913693432228
2108	170-174	-0.3372364951947624
2108	175-179	0.22330585732688135
2108	180-184	-0.43089248813485526
2108	185-189	0.14332726949841046
2108	190-194	-0.33728159230915367
2108	195-199	-0.16721302453027853
2108	200-204	-0.4566775792691473
2108	205-209	0.014173732386009164
2108	210-214	-0.1643357142737667
2108	215-219	-0.1837096279435695
2108	220-224	-0.2653031744725105
2108	225-229	-0.23645118598427572
2108	230-234	-0.23980348694982823
2108	235-239	0.7230308733157056
2108	240-244	-0.22877455142210223
2108	245-249	-0.2233753967187404
2108	250-254	-0.17128404749987958
2108	255-259	1.5334606905205739
2108	260-264	0.18506974285522482
2108	265-269	-0.505144801158643
2108	270-274	-0.2843210884193059
2108	275-279	1.707551586439921
2108	280-284	0.6799625953408448
2108	285-289	0.6273420210696123
2108	290-294	0.4672363339613721
2108	295-299	0.3149972330201809
2108	300-301	-0.4363663704412488
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
9	57.0
10	1856.0
11	13220.0
12	58149.0
13	190343.0
14	569164.0
15	1295963.0
16	2253720.0
17	2824476.0
18	3002393.0
19	2705053.0
20	2058002.0
21	1422137.0
22	1012120.0
23	859508.0
24	878916.0
25	955947.0
26	983726.0
27	885073.0
28	657914.0
29	382420.0
30	165981.0
31	46020.0
32	6180.0
33	510.0
34	195.0
35	84.0
36	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	3.9773103954774047	9.206780793434453	62.21745617788691	24.59845263320123
2	70.06312760610165	20.298095409472758	4.566479861071051	5.072297123354545
3	19.666065984362742	68.62263088517267	4.9797280484770425	6.731575081987538
4	5.502054746753431	77.20669383369338	8.460020474283594	8.831230945269605
5	19.197004279969036	68.85571312584108	4.64835547075785	7.298927123432033
6	9.141019710175534	78.27347395055374	5.357215696637216	7.228290642633511
7	4.487368767859944	6.683158073794431	9.94744502446528	78.88202813388034
8	10.732536805064672	63.967189486997796	5.365157728611563	19.935115979325968
9	9.986809670956394	4.409849008615902	65.76320948601627	19.840131834411434
10-14	20.028563787943128	12.122743344036127	41.4270643846853	26.42162848333544
15-19	23.635995133698447	47.05076468607964	10.738646372616463	18.574593807605446
20-24	21.57364565529685	5.068120836465529	44.80806913809876	28.550164370138866
25-29	21.271468508950395	15.203701041020029	24.78657840970436	38.73825204032521
30-34	26.899365657090456	18.613756146181153	41.479531166104834	13.007347030623553
35-39	18.31631132276098	13.627824361986713	38.491649712387115	29.564214602865196
40-44	12.610900790989248	19.968819336402465	44.907777903099294	22.51250196950899
45-49	19.64150459293801	39.195364098713945	27.881483343765634	13.281647964582415
50-54	19.443512434619795	36.99584606661458	11.873547631869178	31.687093866896447
55-59	6.046278080032235	30.29046017200809	50.30233663407255	13.360925113887125
60-64	6.6462522421947225	25.52873083870405	18.88123489814158	48.943782020959645
65-69	15.95391736413832	17.640720233293184	26.761510757608765	39.64385164495974
70-74	15.562197563431848	34.125237620202405	26.819742798753598	23.49282201761215
75-79	13.32094171372681	45.28379526749591	20.37538626053311	21.019876758244173
80-84	20.401382918179493	34.63999183783816	30.31786884921267	14.64075639476967
85-89	5.07900249428089	16.386672085148223	7.388216696786881	71.14610872378401
90-94	25.79340045810393	28.93172849157099	24.530739829898824	20.74413122042626
95-99	5.108595540847363	15.305977882142669	37.166236349397664	42.419190227612305
100-104	7.989676695178914	39.77611111303603	36.28840751218132	15.94580467960373
105-109	7.591917007550293	20.230008002534884	46.074714708761746	26.103360281153076
110-114	5.318011910505819	31.52101364547324	28.27425711690378	34.88671732711716
115-119	18.17181734127513	28.22462153826592	22.50605386207523	31.09750725838372
120-124	7.675665461102987	49.03396096500163	14.164602211727756	29.125771362167622
125-129	6.093795056572587	43.816713001017035	11.022139683713688	39.067352258696694
130-134	8.611973254397263	43.15626621406577	13.727981480576165	34.5037790509608
135-139	10.901314449731025	34.3698156683218	21.702532155405304	33.02633772654187
140-144	13.110401436954561	29.77955994644138	21.460538745171096	35.649499871432965
145-149	8.011353126927565	26.625565562638553	32.35160656893631	33.01147474149758
150-154	4.752277644805831	28.58528199174495	39.49595384704689	27.166486516402326
155-159	7.429263205377773	27.481344197899954	37.631457801823906	27.457934794898364
160-164	8.401833553556893	27.01044788502212	29.465145011246747	35.12257355017424
165-169	9.124701017870676	36.20188520112778	23.302178923515545	31.371234857485998
170-174	11.381112247146456	33.573789763254396	26.595745825781858	28.449352163817288
175-179	11.134070218569567	40.605821329653125	17.626224077479282	30.633884374298027
180-184	9.924418603900302	37.53098189777154	20.372150845604242	32.172448652723915
185-189	12.14798754647232	28.627511094380974	20.3717053411071	38.852796018039605
190-194	9.387333811916282	30.318675182203535	28.34694850678108	31.947042499099105
195-199	9.29805226717523	30.9181508741102	29.096342391441095	30.687454467273472
200-204	7.399196803303355	25.635272409305188	38.84856829804392	28.116962489347536
205-209	8.972132413123104	25.224171473804844	39.08421477796924	26.719481335102813
210-214	10.563469824444715	34.10550240743444	26.04095647710701	29.29007129101383
215-219	12.145318134936224	33.20601821149267	28.794274713764835	25.85438893980627
220-224	11.242656549440774	31.861684867603156	24.50980265541018	32.38585592754588
225-229	11.407322124964555	33.415305118387764	21.488177065254828	33.68919569139285
230-234	10.993718870233177	28.888599252597164	22.60893488698924	37.50874699018042
235-239	12.10486373657988	31.178038583809	18.90410266811431	37.81299501149682
240-244	9.804328562608736	33.14974835609238	25.029768436737836	32.01615464456105
245-249	10.049521259819306	31.532612769247226	25.865136842981112	32.552729127952354
250-254	11.705138093903072	30.426801589638142	27.386756483525804	30.481303832932984
255-259	16.13898307545866	30.52460633884714	23.02379456163867	30.31261602405553
260-264	13.032728151207454	31.623151674760624	22.786212096713324	32.5579080773186
265-269	14.41394005499334	30.74754008810822	22.82011668749319	32.018403169405246
270-274	15.88919739602761	30.9352787783418	22.04432771763387	31.13119610799672
275-279	16.61922676893787	28.68379703719459	23.107511955144336	31.589464238723195
280-284	18.48528705222386	29.1904694600152	19.796750354852815	32.52749313290813
285-289	22.09857162099607	27.92241484525175	17.846375246118335	32.13263828763384
290-294	24.212311444973476	26.515483094115925	18.693578060069736	30.578627400840862
295-299	22.676998921917846	25.325876199685805	21.123817305031302	30.87330757336505
300-301	52.643094161021374	14.444878428631705	12.646388401725423	20.2656390086215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	2.75
2	1.5
3	0.5
4	1.5
5	1.5
6	1.5
7	4.5
8	5.5
9	6.5
10	10.75
11	11.5
12	11.75
13	15.75
14	26.5
15	35.5
16	44.25
17	83.5
18	135.75
19	198.75
20	353.0
21	719.5
22	1548.75
23	3361.5
24	6580.5
25	8875.75
26	9419.5
27	11702.5
28	17850.25
29	28581.75
30	45082.0
31	67486.0
32	95859.0
33	130332.75
34	170019.0
35	213576.25
36	261302.5
37	311986.25
38	360246.0
39	393903.75
40	408678.75
41	415653.75
42	413880.5
43	412047.75
44	420912.0
45	449190.0
46	494328.0
47	540250.25
48	532169.5
49	434532.5
50	300382.75
51	202693.0
52	145301.0
53	108219.0
54	80426.0
55	59201.0
56	43308.5
57	31382.25
58	23107.0
59	17300.25
60	13418.5
61	10441.75
62	8373.5
63	6833.25
64	5404.25
65	4248.75
66	3305.25
67	2646.5
68	2152.25
69	1804.0
70	1569.75
71	1351.5
72	1160.0
73	993.25
74	857.25
75	760.25
76	663.5
77	560.25
78	484.5
79	434.5
80	376.25
81	315.5
82	261.25
83	210.5
84	181.25
85	148.5
86	129.5
87	124.75
88	122.25
89	116.0
90	105.25
91	107.0
92	118.5
93	105.25
94	106.5
95	104.25
96	119.25
97	141.5
98	186.25
99	328.25
100	371.3333333333297
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	5.331747107297128
7	7.829812037811129
8	0.0
9	0.0
10-14	0.5245161895791235
15-19	0.0
20-24	0.547302224705693
25-29	0.0
30-34	1.7219750709668976E-6
35-39	0.0
40-44	0.0
45-49	0.0
50-54	2.1186148088134127
55-59	0.0
60-64	0.5193605962166485
65-69	0.0
70-74	2.665228243490719
75-79	0.0
80-84	0.0
85-89	0.0
90-94	1.7219750709668976E-6
95-99	8.609875354834488E-7
100-104	1.5972765242277587
105-109	1.7219750709668976E-6
110-114	2.5829626064503464E-6
115-119	6.8879002838675905E-6
120-124	2.6690613599986915E-5
125-129	4.132740170320554E-5
130-134	3.4611698926434643E-4
135-139	5.5103202270940724E-5
140-144	5.596418980642417E-5
145-149	6.629604023222555E-5
150-154	5.6825177341907624E-5
155-159	5.5103202270940724E-5
160-164	7.57669031225435E-5
165-169	0.5273178430195866
170-174	4.907628952255658E-5
175-179	6.026912748384142E-5
180-184	4.7354314451589686E-5
185-189	0.5226469856395889
190-194	3.2717526348371056E-5
195-199	0.5269596722048255
200-204	4.5632339380622786E-5
205-209	1.0595786154803837
210-214	5.6825177341907624E-5
215-219	2.6690613599986915E-5
220-224	2.4107650993536565E-5
225-229	3.874443909675519E-5
230-234	2.927357620643726E-5
235-239	3.3578513883854506E-5
240-244	2.6690613599986915E-5
245-249	2.4107650993536565E-5
250-254	1.8941725780635875E-5
255-259	10.584390123784178
260-264	3.188728467777972
265-269	2.655922690207214
270-274	2.655928717119962
275-279	2.6559295781074974
280-284	2.655922690207214
285-289	2.655923551194749
290-294	2.6559218292196785
295-299	2.6559252731698204
300-301	2.6559269951448914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.322914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14880026016833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.60973365014667	44.10898767408133
2	4.158769750015333	4.004795480430369
3	1.3369525195950769	1.9311797946993643
4	0.7795258871907612	1.501329449599139
5	0.4688218965650652	1.128660592765231
6	0.2980520672504832	0.8610509671904265
7	0.184553031065964	0.6220204921142619
8	0.13915822600057007	0.5360241302608649
9	0.12372610201702924	0.5361537035688435
>10	0.7331785302394023	6.610043451178645
>50	0.07355998887258026	2.449427475381742
>100	0.08355445433970952	7.650343135420575
>500	0.005060563072560622	1.6844774416454191
>1k	0.004130215545145449	3.9163261026219107
>5k	5.603535268115545E-4	1.8992055408651929
>10k+	6.62764556697751E-4	20.5599745681767
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAGAAACCCTCTAGGTAGATGCTTTCGCCTTCGTTTCTCTCTCATGGGTC	913395	3.9321085498645236	No Hit
CAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGCTTCTCTCTCATGAGTC	684589	2.9471129796453934	No Hit
CAGAAACCCTCTAGGTAGACGCCTTCGCCTCAGATTCTCTCTCATGAGTC	434128	1.868893984021793	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	200131	0.8615514823191904	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC	180437	0.7767700397001353	No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATC	165306	0.7116320277031349	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC	123491	0.5316210587219329	No Hit
TGATGGTGGAATTACCGCGGCTGCTGGGTAACGGCGATAGTGTAGATCTC	103513	0.44561701380249114	No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC	84918	0.36556669769091754	No Hit
TGATGGTGGAATTACCGCAGCTGCTGGGTAACGGCGATAGTGTAGATCTC	81593	0.35125277991350523	No Hit
TGATGGTGGAATTACCGCGGGTGCTGGGTAACGGCGATAGTGTAGATCTC	72829	0.3135243061086204	No Hit
TGATGGTGGAATTACCGCAGGTGCTGGGTAACGGCGATAGTGTAGATCTC	69876	0.30081182514720733	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	64581	0.27801718014528304	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC	55386	0.23843327820143148	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTCCGTCTTTAACAAATC	40138	0.17279158849617335	No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC	40088	0.17257634161230248	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC	39945	0.1719607355244318	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC	37746	0.16249417757179127	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC	36760	0.1582495090218579	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCAAAAATC	36700	0.15799121276121286	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCAAATC	36480	0.15704412647218105	No Hit
TAGAAATACACTTGATAGATGCTTTCGCCTTCGTTTCTCTCTCATGGGTC	36094	0.155382420528698	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC	35896	0.1545300428685694	No Hit
CGAAACCATCCTTGGCAAATGCTTTCGCCCCCGCTGGTCTTTGGCAAATC	34562	0.1487872560068948	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC	33858	0.14575657988199303	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGATGTTAGTCTTCCGTAAATC	29502	0.12700427135916353	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC	29389	0.1265178134016154	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA	29153	0.1255018481097449	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCTATAAATC	28844	0.12417162236742299	No Hit
AACTAGAAGGACATATAGGGACACATTTGTATTCAGAAAATGGACTGAAA	28707	0.12358184590561681	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC	27016	0.11630219629310426	No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATA	26259	0.11304335847129941	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCAGAAATC	25971	0.11180353642020326	No Hit
TGAAAACATCCTTGGCAAATGCCTTCGCATTAGTTCGTCTTTAAGAAATC	24166	0.10403312391246511	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	9.384764136769592E-4	0.0	0.0
2	9.51391226709211E-4	0.0	0.0
3	9.556961643866281E-4	2.152468838708622E-5	0.0
4	9.600011020640454E-4	2.152468838708622E-5	0.0
5	9.6861097741888E-4	2.152468838708622E-5	0.0
6	9.772208527737145E-4	3.013456374192071E-5	0.0
7	9.944406034833834E-4	3.013456374192071E-5	0.0
8	0.001003050478838218	3.013456374192071E-5	0.0
9	0.0010202702295478868	3.013456374192071E-5	0.0
10-14	0.0010960371326704302	3.0134563741920712E-5	0.0
15-19	0.0012518758765929345	3.1856538812887605E-5	0.0
20-24	0.001507589174631519	3.443950141933795E-5	0.0
25-29	0.0016944234698314273	3.443950141933795E-5	1.7219750709668974E-6
30-34	0.0019690784936506472	3.443950141933795E-5	1.377580056773518E-5
35-39	0.0022936707945279077	3.443950141933795E-5	8.523776601286143E-5
40-44	0.0026768102478180425	3.616147649030485E-5	1.868342951999084E-4
45-49	0.003214927457495198	3.87444390967552E-5	3.581708147611147E-4
50-54	0.003932991062088395	3.87444390967552E-5	5.854715241287451E-4
55-59	0.004917099815145976	3.87444390967552E-5	8.74763336051184E-4
60-64	0.006151755941029241	3.87444390967552E-5	0.0012501539015219677
65-69	0.007590466112822085	3.87444390967552E-5	0.0017159481582185135
70-74	0.009377876236485724	3.87444390967552E-5	0.0021094194619344494
75-79	0.01173870405878134	3.87444390967552E-5	0.0025907114942696975
80-84	0.014542940461850933	4.132740170320554E-5	0.0031288287039468532
85-89	0.018170280948842706	4.391036430965589E-5	0.00370482936518528
90-94	0.02271973908633725	4.7354314451589686E-5	0.0043101036026301455
95-99	0.028063888719083014	4.7354314451589686E-5	0.004865440563016969
100-104	0.03507491022052474	4.7354314451589686E-5	0.005331234819713515
105-109	0.04429780870062344	4.7354314451589686E-5	0.005904652518345492
110-114	0.05571708638374042	4.7354314451589686E-5	0.006478931204512953
115-119	0.06916829465059834	4.7354314451589686E-5	0.0069602232368482005
120-124	0.08334014948465591	4.7354314451589686E-5	0.007436349343970547
125-129	0.0985305525731904	4.7354314451589686E-5	0.007852206323609053
130-134	0.1152586794000983	4.7354314451589686E-5	0.008218987013725003
135-139	0.13275997303387038	4.7354314451589686E-5	0.008571130915737734
140-144	0.15035080937133274	4.7354314451589686E-5	0.008904333091969826
145-149	0.16870878560291083	4.7354314451589686E-5	0.009234952305595472
150-154	0.18909783143069436	4.7354314451589686E-5	0.009543185843298546
155-159	0.20971331698031004	4.7354314451589686E-5	0.009825589754937119
160-164	0.23031933166703542	4.7354314451589686E-5	0.010064944289801515
165-169	0.2509976693067414	4.7354314451589686E-5	0.010312047712485266
170-174	0.27229075204678266	4.7354314451589686E-5	0.010510935833181943
175-179	0.2934615745567851	4.7354314451589686E-5	0.010703797041130236
180-184	0.31331766910010445	4.7354314451589686E-5	0.01088718738618821
185-189	0.3326020679198628	5.4242214735457274E-5	0.011099851307452623
190-194	0.35048391804431844	5.768616487739107E-5	0.01127463177715576
195-199	0.3669296409595878	6.0269127483841424E-5	0.011465771010033089
200-204	0.384034019339502	6.0269127483841424E-5	0.011629358641774942
205-209	0.40166790505373856	6.0269127483841424E-5	0.011765394672381328
210-214	0.42041763061396165	6.0269127483841424E-5	0.011908318603271581
215-219	0.4384828710834753	6.0269127483841424E-5	0.012037466733594099
220-224	0.4553806124548735	6.0269127483841424E-5	0.012156283013490813
225-229	0.4712279490329818	6.0269127483841424E-5	0.012353449159116524
230-234	0.4862590694274519	6.0269127483841424E-5	0.012405969398781013
235-239	0.49893624989991026	6.285209009029177E-5	0.012496373090006774
240-244	0.5098122444481371	6.54350526967421E-5	0.012690095285490552
245-249	0.5165115884617338	6.88790028386759E-5	0.012860570817516275
250-254	0.5203868933589448	7.232295298060971E-5	0.012905342169361414
255-259	0.523790377086711	7.318394051609315E-5	0.012935476733103335
260-264	0.5288219882440762	9.987455411608007E-5	0.012991440922909759
265-269	0.5329245938506548	1.851123201289415E-4	0.013036212274754896
270-274	0.5362040953733114	4.899019076900823E-4	0.013099925352380673
275-279	0.5387577844035552	7.628349564383355E-4	0.013162777442470967
280-284	0.5404737325617737	8.342969218834617E-4	0.013208409781851588
285-289	0.5414079040377732	8.351579094189453E-4	0.013234239407916093
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAAAC	739080	0.0	317.8645	1
GAAACCC	293490	0.0	304.01804	3
GGTGGAA	70270	0.0	303.3848	5
TGGTGGA	66715	0.0	300.6915	4
TCCTTGG	553240	0.0	290.97055	9
CTCTAGG	308950	0.0	288.09775	9
GAAGACA	173680	0.0	286.97113	2
ATCCTTG	562990	0.0	284.6089	8
ATTCTTG	377595	0.0	283.6018	8
GTGGAAT	75725	0.0	280.856	6
TTCTTGG	379590	0.0	275.24002	9
AAACATC	557830	0.0	274.3332	4
CAGAAAC	330395	0.0	273.49872	1
AGAAACC	329815	0.0	271.64777	2
CATCCTT	593430	0.0	270.90778	7
AAGACAT	177680	0.0	270.49115	3
TGAAGAC	80980	0.0	266.41287	1
AACATCC	513360	0.0	265.75397	5
ACATCCT	584670	0.0	265.4292	6
CGAAGAC	74835	0.0	264.54376	1
>>END_MODULE